Restriction map for
pF1KSDA1001
(Circular) (Six-base) MAPSORT of: pF1KSDA1001.seq Check: 91 from: 1 to: 4712
pF1KSDA1001 4712 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 382 382
Size: 4712
AatII G_ACGT'C
Cuts at: 1298 1592 1298
Size: 294 4418
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2537 2969 3716 3984 1631
Size: 906 432 747 268 2359
Fragments arranged by size:
2359 906 747 432 268
AflIII A'CryG_T
Cuts at: 2064 3168 4145 2064
Size: 1104 977 2631
Fragments arranged by size:
2631 1104 977
AgeI A'CCGG_T
Cuts at: 1426 3100 1426
Size: 1674 3038
AleI CACnn'nnGTG
Cuts at: 454 718 454
Size: 264 4448
AlwNI CAG_nnn'CTG
Cuts at: 1519 2017 3584 1519
Size: 498 1567 2647
Fragments arranged by size:
2647 1567 498
ApaLI G'TGCA_C
Cuts at: 3482 3482
Size: 4712
ApoI r'AATT_y
Cuts at: 1716 4065 4321 1716
Size: 2349 256 2107
Fragments arranged by size:
2349 2107 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 386 1564 1732 386
Size: 1178 168 3366
Fragments arranged by size:
3366 1178 168
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 1145 1728 2142 2390 2425 1145
Size: 583 414 248 35 3432
Fragments arranged by size:
3432 583 414 248 35
BanII G_rGCy'C
Cuts at: 944 1047 1383 1726 2756 944
Size: 103 336 343 1030 2900
Fragments arranged by size:
2900 1030 343 336 103
BbeI G_GCGC'C
Cuts at: 2146 2394 2146
Size: 248 4464
BbsI GAAGACnn'nnnn_
Cuts at: 1508 1508
Size: 4712
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglI GCCn_nnn'nGGC
Cuts at: 413 413
Size: 4712
BglII A'GATC_T
Cuts at: 2227 4264 2227
Size: 2037 2675
BlpI GC'TnA_GC
Cuts at: 1824 1824
Size: 4712
Bme1580I G_kGCm'C
Cuts at: 884 1148 1334 1526 2337 2430 3486 884
Size: 264 186 192 811 93 1056 2110
Fragments arranged by size:
2110 1056 811 264 192 186 93
BmgBI CAC'GTC
Cuts at: 720 720
Size: 4712
BmrI ACTGGGnnnn_n'
Cuts at: 297 530 804 1924 2005 2340 4484 297
Size: 233 274 1120 81 335 2144 525
Fragments arranged by size:
2144 1120 525 335 274 233 81
BmtI G_CTAG'C
Cuts at: 1063 1063
Size: 4712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1388 1420 1605 620
Size: 768 32 185 3727
Fragments arranged by size:
3727 768 185 32
Bpu10I CC'TnA_GC
Cuts at: 1463 1463
Size: 4712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 792 1253 1888 2767 3259 3557 792
Size: 461 635 879 492 298 1947
Fragments arranged by size:
1947 879 635 492 461 298
BsaI GGTCTCn'nnnn_
Cuts at: 472 4416 472
Size: 3944 768
BsaAI yAC'GTr
Cuts at: 879 1704 2065 879
Size: 825 361 3526
Fragments arranged by size:
3526 825 361
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 1295 1589 2143 2391 3093 4589 1295
Size: 294 554 248 702 1496 1418
Fragments arranged by size:
1496 1418 702 554 294 248
BsaWI w'CCGG_w
Cuts at: 1426 1901 2422 3100 3374 3521 3932 1426
Size: 475 521 678 274 147 411 2206
Fragments arranged by size:
2206 678 521 475 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 618 648 1603 1633 618
Size: 30 955 30 3697
Fragments arranged by size:
3697 955 30 30
BseYI C'CCAG_C
Cuts at: 1155 2137 2895 3472 3968 1155
Size: 982 758 577 496 1899
Fragments arranged by size:
1899 982 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 956
Size: 4712
BsiEI CG_ry'CG
Cuts at: 104 408 2254 2300 3508 104
Size: 304 1846 46 1208 1308
Fragments arranged by size:
1846 1308 1208 304 46
BsiHKAI G_wGCw'C
Cuts at: 1310 1456 1726 2504 2694 3486 1310
Size: 146 270 778 190 792 2536
Fragments arranged by size:
2536 792 778 270 190 146
BsmBI CGTCTCn'nnnn_
Cuts at: 1302 1302
Size: 4712
Bsp1286I G_dGCh'C
Cuts at: 884 944 1047 1148 1310 1334 1383 1456 1526
Size: 60 103 101 162 24 49 73 70
Cuts at: 1526 1726 2337 2430 2504 2694 2756 3486 884
Size: 200 611 93 74 190 62 730 2110
Fragments arranged by size:
2110 730 611 200 190 162 103 101
93 74 73 70 62 60 49 24
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 1762 1976 2278 2659 382
Size: 1380 214 302 381 2435
Fragments arranged by size:
2435 1380 381 302 214
BsrBI CCG'CTC
Cuts at: 50 471 3004 3058 4542 4707 50
Size: 421 2533 54 1484 165 55
Fragments arranged by size:
2533 1484 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 429 1601 2624 429
Size: 1172 1023 2517
Fragments arranged by size:
2517 1172 1023
BsrFI r'CCGG_y
Cuts at: 403 407 762 1316 1426 2710 2891 3100 403
Size: 4 355 554 110 1284 181 209 2015
Fragments arranged by size:
2015 1284 554 355 209 181 110 4
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 1338 2089 1338
Size: 751 3961
BstBI TT'CG_AA
Cuts at: 110 3073 110
Size: 2963 1749
BstEII G'GTnAC_C
Cuts at: 271 271
Size: 4712
BstYI r'GATC_y
Cuts at: 1 1737 2204 2227 2562 2808 3820 3906 3918
Size: 1736 467 23 335 246 1012 86 12
Cuts at: 3918 4264 1
Size: 346 449
Fragments arranged by size:
1736 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 552 576 913 1039 125
Size: 427 24 337 126 3798
Fragments arranged by size:
3798 427 337 126 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 305 1074 1202 1955 305
Size: 769 128 753 3062
Fragments arranged by size:
3062 769 753 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 558 593 637 672 558
Size: 35 44 35 4598
Fragments arranged by size:
4598 44 35 35
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DraIII CAC_nnn'GTG
Cuts at: 676 996 1338 676
Size: 320 342 4050
Fragments arranged by size:
4050 342 320
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3276 2418
Size: 858 3854
EaeI y'GGCC_r
Cuts at: 251 405 1314 2297 2471 2862 2889 4234 251
Size: 154 909 983 174 391 27 1345 729
Fragments arranged by size:
1345 983 909 729 391 174 154 27
EagI C'GGCC_G
Cuts at: 405 2297 405
Size: 1892 2820
EarI CTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1384 2920 3230 3376 4522 1384
Size: 1536 310 146 1146 1574
Fragments arranged by size:
1574 1536 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1724 1724
Size: 4712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1388 1420 1605 1631 2537 2969 3716 3984
Size: 768 32 185 26 906 432 747 268
Cuts at: 3984 620
Size: 1348
Fragments arranged by size:
1348 906 768 747 432 268 185 32
26
EcoNI CCTnn'n_nnAGG
Cuts at: 1252 1252
Size: 4712
EcoO109I rG'GnC_Cy
Cuts at: 842 1075 1152 1459 1641 1851 842
Size: 233 77 307 182 210 3703
Fragments arranged by size:
3703 307 233 210 182 77
EcoRI G'AATT_C
Cuts at: 1716 1716
Size: 4712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 2731 252
Size: 2479 2233
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 913 1281 2146 2394 3416 4395 913
Size: 368 865 248 1022 979 1230
Fragments arranged by size:
1230 1022 979 865 368 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 942 974 1603 1635 2554 2584 2586
Size: 32 240 32 629 32 919 30 2
Cuts at: 2586 2616 670
Size: 30 2766
Fragments arranged by size:
2766 919 629 240 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 987 1751 987
Size: 764 3948
KasI G'GCGC_C
Cuts at: 2142 2390 2142
Size: 248 4464
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1258 3383 3567 1258
Size: 2125 184 2403
Fragments arranged by size:
2403 2125 184
MscI TGG'CCA
Cuts at: 2473 2473
Size: 4712
MslI CAynn'nnrTG
Cuts at: 454 718 877 2701 2828 454
Size: 264 159 1824 127 2338
Fragments arranged by size:
2338 1824 264 159 127
MspA1I CmG'CkG
Cuts at: 364 836 1358 1366 1688 1823 2137 2497 3510
Size: 472 522 8 322 135 314 360 1013
Cuts at: 3510 3755 364
Size: 245 1321
Fragments arranged by size:
1321 1013 522 472 360 322 314 245
135 8
NaeI GCC'GGC
Cuts at: 409 2893 409
Size: 2484 2228
NarI GG'CG_CC
Cuts at: 2143 2391 2143
Size: 248 4464
NcoI C'CATG_G
Cuts at: 125 125
Size: 4712
NgoMIV G'CCGG_C
Cuts at: 407 2891 407
Size: 2484 2228
NheI G'CTAG_C
Cuts at: 1059 1059
Size: 4712
NspI r_CATG'y
Cuts at: 876 1765 2265 3172 876
Size: 889 500 907 2416
Fragments arranged by size:
2416 907 889 500
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4712
PflMI CCAn_nnn'nTGG
Cuts at: 131 618 131
Size: 487 4225
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PmlI CAC'GTG
Cuts at: 879 879
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 1075 1152 1641 1075
Size: 77 489 4146
Fragments arranged by size:
4146 489 77
PstI C_TGCA'G
Cuts at: 503 1388 1472 1759 2444 503
Size: 885 84 287 685 2771
Fragments arranged by size:
2771 885 685 287 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 364 1366 2137 2497 364
Size: 1002 771 360 2579
Fragments arranged by size:
2579 1002 771 360
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 1726 1726
Size: 4712
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SanDI GG'GwC_CC
Cuts at: 1075 1152 1075
Size: 77 4635
SapI GCTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 4229 4229
Size: 4712
SfcI C'TryA_G
Cuts at: 33 499 1384 1468 1755 2440 3433 3624 33
Size: 466 885 84 287 685 993 191 1121
Fragments arranged by size:
1121 993 885 685 466 287 191 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 413 413
Size: 4712
SfoI GGC'GCC
Cuts at: 2144 2392 2144
Size: 248 4464
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 388 1734 388
Size: 1346 3366
SmlI C'TyrA_G
Cuts at: 771 1268 1867 2782 3274 3536 771
Size: 497 599 915 492 262 1947
Fragments arranged by size:
1947 915 599 497 492 262
SnaBI TAC'GTA
Cuts at: 1704 1704
Size: 4712
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
StuI AGG'CCT
Cuts at: 464 1414 464
Size: 950 3762
StyI C'CwwG_G
Cuts at: 125 485 1846 2200 125
Size: 360 1361 354 2637
Fragments arranged by size:
2637 1361 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2295 2428 2887 3096 2295
Size: 133 459 209 3911
Fragments arranged by size:
3911 459 209 133
TatI w'GTAC_w
Cuts at: 625 4227 625
Size: 3602 1110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 4680
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 2207
Size: 4712
XmaI C'CCGG_G
Cuts at: 386 1732 386
Size: 1346 3366
ZraI GAC'GTC
Cuts at: 1296 1590 1296
Size: 294 4418
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BseRI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SgrAI SrfI SspI SwaI XhoI XmnI