Restriction map for
pF1KSDA1014
(Circular) (Six-base) MAPSORT of: pF1KSDA1014.seq Check: 6973 from: 1 to: 6190
pF1KSDA1014 6190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2739 2739
Size: 6190
AccI GT'mk_AC
Cuts at: 3226 3226
Size: 6190
Acc65I G'GTAC_C
Cuts at: 147 3204 147
Size: 3057 3133
AclI AA'CG_TT
Cuts at: 6030 6030
Size: 6190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1855 2197 2399 3169 4013 4445 5194 5462
Size: 1538 342 202 770 844 432 749 268
Cuts at: 5462 317
Size: 1045
Fragments arranged by size:
1538 1045 844 770 749 432 342 268
202
AflIII A'CryG_T
Cuts at: 3540 4646 5623 3540
Size: 1106 977 4107
Fragments arranged by size:
4107 1106 977
AgeI A'CCGG_T
Cuts at: 4578 4578
Size: 6190
AleI CACnn'nnGTG
Cuts at: 298 2521 298
Size: 2223 3967
AlwNI CAG_nnn'CTG
Cuts at: 613 925 2731 3081 3493 5062 613
Size: 312 1806 350 412 1569 1741
Fragments arranged by size:
1806 1741 1569 412 350 312
ApaLI G'TGCA_C
Cuts at: 4960 4960
Size: 6190
ApoI r'AATT_y
Cuts at: 1046 1596 1617 1705 1744 2103 3192 5543 5799
Size: 550 21 88 39 359 1089 2351 256
Cuts at: 5799 1046
Size: 1437
Fragments arranged by size:
2351 1437 1089 550 359 256 88 39
21
AseI AT'TA_AT
Cuts at: 20 1763 20
Size: 1743 4447
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6190
AvaI C'yCGr_G
Cuts at: 140 191 2804 3208 140
Size: 51 2613 404 3122
Fragments arranged by size:
3122 2613 404 51
BamHI G'GATC_C
Cuts at: 3213 3213
Size: 6190
BanI G'GyrC_C
Cuts at: 147 3204 3618 3866 3901 147
Size: 3057 414 248 35 2436
Fragments arranged by size:
3057 2436 414 248 35
BanII G_rGCy'C
Cuts at: 1842 2375 3202 4232 1842
Size: 533 827 1030 3800
Fragments arranged by size:
3800 1030 827 533
BbeI G_GCGC'C
Cuts at: 3622 3870 3622
Size: 248 5942
BbsI GAAGACnn'nnnn_
Cuts at: 1271 1271
Size: 6190
BbvCI CC'TCA_GC
Cuts at: 599 599
Size: 6190
BciVI GTATCCnnnnn_n'
Cuts at: 2992 4086 4849 6190 2992
Size: 1094 763 1341 2992
Fragments arranged by size:
2992 1341 1094 763
BclI T'GATC_A
Cuts at: 3708 3708
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 280 313 280
Size: 33 6157
BglII A'GATC_T
Cuts at: 3703 5742 3703
Size: 2039 4151
BlpI GC'TnA_GC
Cuts at: 3300 3300
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 3813 3906 4964 3813
Size: 93 1058 5039
Fragments arranged by size:
5039 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 902 1947 3400 3481 3816 5962 902
Size: 1045 1453 81 335 2146 1130
Fragments arranged by size:
2146 1453 1130 1045 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 4555 261
Size: 4294 1896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1366 1547 1634 2264 2540 1325
Size: 41 181 87 630 276 4975
Fragments arranged by size:
4975 630 276 181 87 41
Bpu10I CC'TnA_GC
Cuts at: 599 599
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 3364 4243 4737 5035 1436
Size: 1928 879 494 298 2591
Fragments arranged by size:
2591 1928 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5894 5894
Size: 6190
BsaAI yAC'GTr
Cuts at: 3180 3541 3180
Size: 361 5829
BsaBI GATnn'nnATC
Cuts at: 6 3702 5741 6
Size: 3696 2039 455
Fragments arranged by size:
3696 2039 455
BsaHI Gr'CG_yC
Cuts at: 3619 3867 4571 6067 3619
Size: 248 704 1496 3742
Fragments arranged by size:
3742 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3377 3898 4578 4852 4999 5410 3377
Size: 521 680 274 147 411 4157
Fragments arranged by size:
4157 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1527 1557 1527
Size: 30 6160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 587 1084 1087 1186 1330 2291 2303 2383 2843
Size: 497 3 99 144 961 12 80 460
Cuts at: 2843 2846 2849 2852 2855 587
Size: 3 3 3 3 3922
Fragments arranged by size:
3922 961 497 460 144 99 80 12
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 262 3613 4371 4950 5446 262
Size: 3351 758 579 496 1006
Fragments arranged by size:
3351 1006 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 3029 382
Size: 2647 3543
BsiEI CG_ry'CG
Cuts at: 104 156 3730 3776 4986 104
Size: 52 3574 46 1210 1308
Fragments arranged by size:
3574 1308 1210 52 46
BsiHKAI G_wGCw'C
Cuts at: 3202 3980 4170 4964 3202
Size: 778 190 794 4428
Fragments arranged by size:
4428 794 778 190
BsmI GAATG_Cn'
Cuts at: 1466 1466
Size: 6190
Bsp1286I G_dGCh'C
Cuts at: 1842 2375 3202 3813 3906 3980 4170 4232 4964
Size: 533 827 611 93 74 190 62 732
Cuts at: 4964 1842
Size: 3068
Fragments arranged by size:
3068 827 732 611 533 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5410 5410
Size: 6190
BspHI T'CATG_A
Cuts at: 404 5366 404
Size: 4962 1228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2739 3238 3452 3754 4135 2739
Size: 499 214 302 381 4794
Fragments arranged by size:
4794 499 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4480 4534 6020 6185 50
Size: 4430 54 1486 165 55
Fragments arranged by size:
4430 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2785 4100 2785
Size: 1315 4875
BsrFI r'CCGG_y
Cuts at: 4186 4367 4578 4186
Size: 181 211 5798
Fragments arranged by size:
5798 211 181
BssSI C'ACGA_G
Cuts at: 4459 4819 4459
Size: 360 5830
BstAPI GCAn_nnn'nTGC
Cuts at: 1588 2731 3565 1588
Size: 1143 834 4213
Fragments arranged by size:
4213 1143 834
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6190
BstEII G'GTnAC_C
Cuts at: 2590 2590
Size: 6190
BstXI CCAn_nnnn'nTGG
Cuts at: 724 724
Size: 6190
BstYI r'GATC_y
Cuts at: 1 1281 2333 3213 3680 3703 4038 4284 5298
Size: 1280 1052 880 467 23 335 246 1014
Cuts at: 5298 5384 5396 5742 1
Size: 86 12 346 449
Fragments arranged by size:
1280 1052 1014 880 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1118 3128 1118
Size: 2010 4180
BtgI C'CryG_G
Cuts at: 125 251 296 311 125
Size: 126 45 15 6004
Fragments arranged by size:
6004 126 45 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1079 4138 4319 4482 1079
Size: 3059 181 163 2787
Fragments arranged by size:
3059 2787 181 163
BtsI GCAGTG_nn'
Cuts at: 1296 2516 2528 3431 1296
Size: 1220 12 903 4055
Fragments arranged by size:
4055 1220 903 12
ClaI AT'CG_AT
Cuts at: 581 581
Size: 6190
DraI TTT'AAA
Cuts at: 3187 3187
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 3894 4754 3894
Size: 860 5330
EaeI y'GGCC_r
Cuts at: 153 560 3773 3947 4338 4365 5712 153
Size: 407 3213 174 391 27 1347 631
Fragments arranged by size:
3213 1347 631 407 391 174 27
EagI C'GGCC_G
Cuts at: 153 3773 153
Size: 3620 2570
EarI CTCTTCn'nnn_
Cuts at: 1133 1316 2009 2932 2996 3020 3056 3145 4211
Size: 183 693 923 64 24 36 89 1066
Cuts at: 4211 4421 1133
Size: 210 2902
Fragments arranged by size:
2902 1066 923 693 210 183 89 64
36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 170 4396 4708 4854 6000 170
Size: 4226 312 146 1146 360
Fragments arranged by size:
4226 1146 360 312 146
EcoICRI GAG'CTC
Cuts at: 3200 3200
Size: 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1325 1366 1547 1634 1855 2197 2264 2399
Size: 1008 41 181 87 221 342 67 135
Cuts at: 2399 2540 3169 4013 4445 5194 5462 317
Size: 141 629 844 432 749 268 1045
Fragments arranged by size:
1045 1008 844 749 629 432 342 268
221 181 141 135 87 67 41
EcoNI CCTnn'n_nnAGG
Cuts at: 335 2381 3127 335
Size: 2046 746 3398
Fragments arranged by size:
3398 2046 746
EcoO109I rG'GnC_Cy
Cuts at: 187 213 3327 187
Size: 26 3114 3050
Fragments arranged by size:
3114 3050 26
EcoRI G'AATT_C
Cuts at: 1046 1617 3192 1046
Size: 571 1575 4044
Fragments arranged by size:
4044 1575 571
EcoRV GAT'ATC
Cuts at: 1434 1434
Size: 6190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1354 1858 4207 1354
Size: 504 2349 3337
Fragments arranged by size:
3337 2349 504
FspI TGC'GCA
Cuts at: 3969 3969
Size: 6190
HaeII r_GCGC'y
Cuts at: 3622 3870 4894 5873 3622
Size: 248 1024 979 3939
Fragments arranged by size:
3939 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1760 1792 1797 1829 3022 3054 4030 4060 4062
Size: 32 5 32 1193 32 976 30 2
Cuts at: 4062 4092 1760
Size: 30 3858
Fragments arranged by size:
3858 1193 976 32 32 32 30 30
5 2
HincII GTy'rAC
Cuts at: 539 3227 539
Size: 2688 3502
KasI G'GCGC_C
Cuts at: 3618 3866 3618
Size: 248 5942
KpnI G_GTAC'C
Cuts at: 151 3208 151
Size: 3057 3133
MfeI C'AATT_G
Cuts at: 1583 2765 1583
Size: 1182 5008
MluI A'CGCG_T
Cuts at: 5623 5623
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 661 744 1232 1805 4861 5045 661
Size: 83 488 573 3056 184 1806
Fragments arranged by size:
3056 1806 573 488 184 83
MscI TGG'CCA
Cuts at: 562 3949 562
Size: 3387 2803
MslI CAynn'nnrTG
Cuts at: 298 2521 4177 4304 298
Size: 2223 1656 127 2184
Fragments arranged by size:
2223 2184 1656 127
MspA1I CmG'CkG
Cuts at: 253 298 313 424 3081 3299 3613 3973 4988
Size: 45 15 111 2657 218 314 360 1015
Cuts at: 4988 5233 253
Size: 245 1210
Fragments arranged by size:
2657 1210 1015 360 314 245 218 111
45 15
NaeI GCC'GGC
Cuts at: 4369 4369
Size: 6190
NarI GG'CG_CC
Cuts at: 3619 3867 3619
Size: 248 5942
NcoI C'CATG_G
Cuts at: 125 125
Size: 6190
NgoMIV G'CCGG_C
Cuts at: 4367 4367
Size: 6190
NruI TCG'CGA
Cuts at: 4551 4551
Size: 6190
NspI r_CATG'y
Cuts at: 3241 3741 4650 3241
Size: 500 909 4781
Fragments arranged by size:
4781 909 500
PciI A'CATG_T
Cuts at: 4646 4646
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1570 1570
Size: 6190
PfoI T'CCnGG_A
Cuts at: 2336 2336
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3187 3187
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5329 5361 5329
Size: 32 6158
PpuMI rG'GwC_Cy
Cuts at: 187 213 187
Size: 26 6164
PstI C_TGCA'G
Cuts at: 609 2523 2702 3235 3920 609
Size: 1914 179 533 685 2879
Fragments arranged by size:
2879 1914 685 533 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6190
PvuII CAG'CTG
Cuts at: 424 3081 3613 3973 424
Size: 2657 532 360 2641
Fragments arranged by size:
2657 2641 532 360
RsrII CG'GwC_CG
Cuts at: 4383 4383
Size: 6190
SacI G_AGCT'C
Cuts at: 3202 3202
Size: 6190
SacII CC_GC'GG
Cuts at: 254 299 314 254
Size: 45 15 6130
Fragments arranged by size:
6130 45 15
SalI G'TCGA_C
Cuts at: 3225 3225
Size: 6190
SanDI GG'GwC_CC
Cuts at: 213 213
Size: 6190
SapI GCTCTTCn'nnn_
Cuts at: 4211 4421 4211
Size: 210 5980
SbfI CC_TGCA'GG
Cuts at: 3235 3235
Size: 6190
ScaI AGT'ACT
Cuts at: 5707 5707
Size: 6190
SfcI C'TryA_G
Cuts at: 33 605 614 2504 2519 2565 2579 2585 2698
Size: 572 9 1890 15 46 14 6 113
Cuts at: 2698 3231 3916 4911 5102 33
Size: 533 685 995 191 1121
Fragments arranged by size:
1890 1121 995 685 572 533 191 113
46 15 14 9 6
SfoI GGC'GCC
Cuts at: 3620 3868 3620
Size: 248 5942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6190
SmaI CCC'GGG
Cuts at: 142 3210 142
Size: 3068 3122
SmlI C'TyrA_G
Cuts at: 1451 3343 4258 4752 5014 1451
Size: 1892 915 494 262 2627
Fragments arranged by size:
2627 1892 915 494 262
SnaBI TAC'GTA
Cuts at: 3180 3180
Size: 6190
SpeI A'CTAG_T
Cuts at: 67 931 67
Size: 864 5326
SphI G_CATG'C
Cuts at: 3241 3741 3241
Size: 500 5690
StyI C'CwwG_G
Cuts at: 125 1385 2249 2712 3322 3676 125
Size: 1260 864 463 610 354 2639
Fragments arranged by size:
2639 1260 864 610 463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 730 3771 3904 4363 4574 730
Size: 3041 133 459 211 2346
Fragments arranged by size:
3041 2346 459 211 133
TatI w'GTAC_w
Cuts at: 553 1201 1665 5705 553
Size: 648 464 4040 1038
Fragments arranged by size:
4040 1038 648 464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5707 5739 5707
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 3985 3985
Size: 6190
XbaI T'CTAG_A
Cuts at: 3219 3219
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3683 3683
Size: 6190
XmaI C'CCGG_G
Cuts at: 140 3208 140
Size: 3068 3122
XmnI GAAnn'nnTTC
Cuts at: 1046 3122 1046
Size: 2076 4114
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I CspCI DraIII FseI FspAI HindIII
HpaI NdeI NheI NotI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI