Restriction map for
pF1KSDA1020
(Circular) (Six-base) MAPSORT of: pF1KSDA1020.seq Check: 8974 from: 1 to: 4982
pF1KSDA1020 4982 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1955 1955
Size: 4982
AatII G_ACGT'C
Cuts at: 269 269
Size: 4982
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 4822 4822
Size: 4982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 1819 2807 3239 3986 4254 456
Size: 1363 988 432 747 268 1184
Fragments arranged by size:
1363 1184 988 747 432 268
AflIII A'CryG_T
Cuts at: 1400 1771 2334 3438 4415 1400
Size: 371 563 1104 977 1967
Fragments arranged by size:
1967 1104 977 563 371
AgeI A'CCGG_T
Cuts at: 3370 3370
Size: 4982
AleI CACnn'nnGTG
Cuts at: 150 1865 150
Size: 1715 3267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 543 1514 457
Size: 86 971 3925
Fragments arranged by size:
3925 971 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1516 1548 1516
Size: 32 4950
AlwNI CAG_nnn'CTG
Cuts at: 222 618 858 2287 3854 222
Size: 396 240 1429 1567 1350
Fragments arranged by size:
1567 1429 1350 396 240
ApaI G_GGCC'C
Cuts at: 140 188 303 681 1196 1286 140
Size: 48 115 378 515 90 3836
Fragments arranged by size:
3836 515 378 115 90 48
ApaLI G'TGCA_C
Cuts at: 1870 3752 1870
Size: 1882 3100
ApoI r'AATT_y
Cuts at: 1986 4335 4591 1986
Size: 2349 256 2377
Fragments arranged by size:
2377 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 511 1209 1875 2002 511
Size: 698 666 127 3491
Fragments arranged by size:
3491 698 666 127
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4982
BanI G'GyrC_C
Cuts at: 1024 1086 1573 1762 1998 2412 2660 2695 1024
Size: 62 487 189 236 414 248 35 3311
Fragments arranged by size:
3311 487 414 248 236 189 62 35
BanII G_rGCy'C
Cuts at: 140 188 303 467 659 681 698 1040 1196
Size: 48 115 164 192 22 17 342 156
Cuts at: 1196 1286 1298 1631 1996 3026 140
Size: 90 12 333 365 1030 2096
Fragments arranged by size:
2096 1030 365 342 333 192 164 156
115 90 48 22 17 12
BbeI G_GCGC'C
Cuts at: 1090 2416 2664 1090
Size: 1326 248 3408
Fragments arranged by size:
3408 1326 248
BbsI GAAGACnn'nnnn_
Cuts at: 1352 1666 1705 1352
Size: 314 39 4629
Fragments arranged by size:
4629 314 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1108 1142 1108
Size: 34 4948
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3641 4982 2880
Size: 761 1341 2880
Fragments arranged by size:
2880 1341 761
BclI T'GATC_A
Cuts at: 2502 2502
Size: 4982
BglI GCCn_nnn'nGGC
Cuts at: 788 959 1095 1621 1793 788
Size: 171 136 526 172 3977
Fragments arranged by size:
3977 526 172 171 136
BglII A'GATC_T
Cuts at: 410 2497 4534 410
Size: 2087 2037 858
Fragments arranged by size:
2087 2037 858
BlpI GC'TnA_GC
Cuts at: 943 1625 2094 943
Size: 682 469 3831
Fragments arranged by size:
3831 682 469
Bme1580I G_kGCm'C
Cuts at: 140 188 303 681 1027 1196 1286 1874 2607
Size: 48 115 378 346 169 90 588 733
Cuts at: 2607 2700 3756 140
Size: 93 1056 1366
Fragments arranged by size:
1366 1056 733 588 378 346 169 115
93 90 48
BmrI ACTGGGnnnn_n'
Cuts at: 1601 2194 2275 2610 4754 1601
Size: 593 81 335 2144 1829
Fragments arranged by size:
2144 1829 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1069 1102 487
Size: 582 33 4367
Fragments arranged by size:
4367 582 33
Bpu10I CC'TnA_GC
Cuts at: 1072 1072
Size: 4982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 2158 3037 3529 3827 526
Size: 1632 879 492 298 1681
Fragments arranged by size:
1681 1632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4686 4686
Size: 4982
BsaAI yAC'GTr
Cuts at: 1506 1974 2335 1506
Size: 468 361 4153
Fragments arranged by size:
4153 468 361
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 266 1087 2413 2661 3363 4859 266
Size: 821 1326 248 702 1496 389
Fragments arranged by size:
1496 1326 821 702 389 248
BsaWI w'CCGG_w
Cuts at: 149 2171 2692 3370 3644 3791 4202 149
Size: 2022 521 678 274 147 411 929
Fragments arranged by size:
2022 929 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 1231 1330 1333 1336 1594 1597 472
Size: 759 99 3 3 258 3 3857
Fragments arranged by size:
3857 759 258 99 3 3 3
BseYI C'CCAG_C
Cuts at: 202 445 783 858 938 1017 1480 1674 1922
Size: 243 338 75 80 79 463 194 248
Cuts at: 1922 2407 3165 3742 4238 202
Size: 485 758 577 496 946
Fragments arranged by size:
946 758 577 496 485 463 338 248
243 194 80 79 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 4982
BsiEI CG_ry'CG
Cuts at: 104 2524 2570 3778 104
Size: 2420 46 1208 1308
Fragments arranged by size:
2420 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 698 1862 1874 1996 2774 2964 3756 698
Size: 1164 12 122 778 190 792 1924
Fragments arranged by size:
1924 1164 792 778 190 122 12
BsmI GAATG_Cn'
Cuts at: 1457 1457
Size: 4982
BsmBI CGTCTCn'nnnn_
Cuts at: 1417 1417
Size: 4982
Bsp1286I G_dGCh'C
Cuts at: 140 188 303 467 659 681 698 1027 1040
Size: 48 115 164 192 22 17 329 13
Cuts at: 1040 1196 1286 1298 1631 1862 1874 1996 2607
Size: 156 90 12 333 231 12 122 611
Cuts at: 2607 2700 2774 2964 3026 3756 140
Size: 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 611 333 329 231 192 190
164 156 122 115 93 90 74 62
48 22 17 13 12 12
BspEI T'CCGG_A
Cuts at: 4202 4202
Size: 4982
BspHI T'CATG_A
Cuts at: 4158 4158
Size: 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1955 2032 2246 2548 2929 1955
Size: 77 214 302 381 4008
Fragments arranged by size:
4008 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 1179 1361 3274 3328 4812 4977 50
Size: 1129 182 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 1129 182 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2894 2894
Size: 4982
BsrFI r'CCGG_y
Cuts at: 550 1406 2980 3161 3370 550
Size: 856 1574 181 209 2162
Fragments arranged by size:
2162 1574 856 209 181
BssSI C'ACGA_G
Cuts at: 658 1693 3253 3611 658
Size: 1035 1560 358 2029
Fragments arranged by size:
2029 1560 1035 358
BstAPI GCAn_nnn'nTGC
Cuts at: 222 1463 1609 2359 222
Size: 1241 146 750 2845
Fragments arranged by size:
2845 1241 750 146
BstBI TT'CG_AA
Cuts at: 110 3343 110
Size: 3233 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 591 1047 591
Size: 456 4526
BstYI r'GATC_y
Cuts at: 1 410 1044 2007 2474 2497 2832 3078 4090
Size: 409 634 963 467 23 335 246 1012
Cuts at: 4090 4176 4188 4534 1
Size: 86 12 346 449
Fragments arranged by size:
1012 963 634 467 449 409 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 623 1040 125
Size: 498 417 4067
Fragments arranged by size:
4067 498 417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1829 2932 3113 3276 1829
Size: 1103 181 163 3535
Fragments arranged by size:
3535 1103 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1572 2225 485
Size: 1087 653 3242
Fragments arranged by size:
3242 1087 653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1719 1754 1719
Size: 35 4947
DraI TTT'AAA
Cuts at: 1981 1981
Size: 4982
DraIII CAC_nnn'GTG
Cuts at: 1609 1609
Size: 4982
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3546 2688
Size: 858 4124
EaeI y'GGCC_r
Cuts at: 1380 2567 2741 3132 3159 4504 1380
Size: 1187 174 391 27 1345 1858
Fragments arranged by size:
1858 1345 1187 391 174 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 1102 1520 1538 3005 3215 1102
Size: 418 18 1467 210 2869
Fragments arranged by size:
2869 1467 418 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3190 3500 3646 4792 3190
Size: 310 146 1146 3380
Fragments arranged by size:
3380 1146 310 146
EcoICRI GAG'CTC
Cuts at: 696 1994 696
Size: 1298 3684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 487 1069 1102 1819 2807 3239 3986 4254
Size: 31 582 33 717 988 432 747 268
Cuts at: 4254 456
Size: 1184
Fragments arranged by size:
1184 988 747 717 582 432 268 33
31
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1081
Size: 4982
EcoO109I rG'GnC_Cy
Cuts at: 137 184 677 910 1192 1201 1283 1550 1593
Size: 47 493 233 282 9 82 267 43
Cuts at: 1593 1671 2121 137
Size: 78 450 2998
Fragments arranged by size:
2998 493 450 282 267 233 82 78
47 43 9
EcoRI G'AATT_C
Cuts at: 1986 1986
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4982
FspI TGC'GCA
Cuts at: 1355 1502 1951 2763 1355
Size: 147 449 812 3574
Fragments arranged by size:
3574 812 449 147
FspAI rTGC'GCAy
Cuts at: 1502 1502
Size: 4982
HaeII r_GCGC'y
Cuts at: 1090 1394 2416 2664 3686 4665 1090
Size: 304 1022 248 1022 979 1407
Fragments arranged by size:
1407 1022 1022 979 304 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1414 1446 1582 1614 2824 2854 2856 2886 1414
Size: 32 136 32 1210 30 2 30 3510
Fragments arranged by size:
3510 1210 136 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2021 2021
Size: 4982
KasI G'GCGC_C
Cuts at: 1086 2412 2660 1086
Size: 1326 248 3408
Fragments arranged by size:
3408 1326 248
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 1771 4415 1771
Size: 2644 2338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3653 3837 3653
Size: 184 4798
MscI TGG'CCA
Cuts at: 2743 2743
Size: 4982
MslI CAynn'nnrTG
Cuts at: 150 274 277 382 589 1865 1867 2971 3098
Size: 124 3 105 207 1276 2 1104 127
Cuts at: 3098 150
Size: 2034
Fragments arranged by size:
2034 1276 1104 207 127 124 105 3
2
MspA1I CmG'CkG
Cuts at: 231 507 615 787 839 942 1379 1902 2093
Size: 276 108 172 52 103 437 523 191
Cuts at: 2093 2407 2767 3780 4025 231
Size: 314 360 1013 245 1188
Fragments arranged by size:
1188 1013 523 437 360 314 276 245
191 172 108 103 52
NaeI GCC'GGC
Cuts at: 552 3163 552
Size: 2611 2371
NarI GG'CG_CC
Cuts at: 1087 2413 2661 1087
Size: 1326 248 3408
Fragments arranged by size:
3408 1326 248
NcoI C'CATG_G
Cuts at: 125 1040 125
Size: 915 4067
NgoMIV G'CCGG_C
Cuts at: 550 3161 550
Size: 2611 2371
NspI r_CATG'y
Cuts at: 1404 1782 1830 1866 1956 2035 2535 3442 1404
Size: 378 48 36 90 79 500 907 2944
Fragments arranged by size:
2944 907 500 378 90 79 48 36
PciI A'CATG_T
Cuts at: 1400 3438 1400
Size: 2038 2944
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PmlI CAC'GTG
Cuts at: 1506 1506
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4950
PpuMI rG'GwC_Cy
Cuts at: 910 1201 1593 1671 910
Size: 291 392 78 4221
Fragments arranged by size:
4221 392 291 78
PspOMI G'GGCC_C
Cuts at: 136 184 299 677 1192 1282 136
Size: 48 115 378 515 90 3836
Fragments arranged by size:
3836 515 378 115 90 48
PstI C_TGCA'G
Cuts at: 230 817 2029 2714 230
Size: 587 1212 685 2498
Fragments arranged by size:
2498 1212 685 587
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 231 507 787 942 1902 2407 2767 231
Size: 276 280 155 960 505 360 2446
Fragments arranged by size:
2446 960 505 360 280 276 155
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 698 1996 698
Size: 1298 3684
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SanDI GG'GwC_CC
Cuts at: 1201 1201
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 1102 1520 3005 3215 1102
Size: 418 1485 210 2869
Fragments arranged by size:
2869 1485 418 210
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 4499 4499
Size: 4982
SfcI C'TryA_G
Cuts at: 33 226 813 2025 2710 3703 3894 33
Size: 193 587 1212 685 993 191 1121
Fragments arranged by size:
1212 1121 993 685 587 193 191
SfoI GGC'GCC
Cuts at: 1088 2414 2662 1088
Size: 1326 248 3408
Fragments arranged by size:
3408 1326 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 1211 2004 1211
Size: 793 4189
SmlI C'TyrA_G
Cuts at: 541 2137 3052 3544 3806 541
Size: 1596 915 492 262 1717
Fragments arranged by size:
1717 1596 915 492 262
SnaBI TAC'GTA
Cuts at: 1974 1974
Size: 4982
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 1830 1956 2035 2535 1830
Size: 126 79 500 4277
Fragments arranged by size:
4277 500 126 79
StyI C'CwwG_G
Cuts at: 125 140 248 1040 1196 1469 2116 2470 125
Size: 15 108 792 156 273 647 354 2637
Fragments arranged by size:
2637 792 647 354 273 156 108 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1638 1936 2565 2698 3157 3366 1620
Size: 18 298 629 133 459 209 3236
Fragments arranged by size:
3236 629 459 298 209 133 18
TatI w'GTAC_w
Cuts at: 4497 4497
Size: 4982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 894 926 1706 1738 4499 4531 894
Size: 32 780 32 2761 32 1345
Fragments arranged by size:
2761 1345 780 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 385 1428 2779 385
Size: 1043 1351 2588
Fragments arranged by size:
2588 1351 1043
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1254 2477 1254
Size: 1223 3759
XmaI C'CCGG_G
Cuts at: 1209 2002 1209
Size: 793 4189
XmnI GAAnn'nnTTC
Cuts at: 728 1804 728
Size: 1076 3906
ZraI GAC'GTC
Cuts at: 267 267
Size: 4982
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BbvCI BfrBI
BmgBI BmtI BsaXI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI