Restriction map for
pF1KSDA1027
(Circular) (Six-base) MAPSORT of: pF1KSDA1027.seq Check: 6794 from: 1 to: 10760
pF1KSDA1027 10760 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3490 6630 3490
Size: 3140 7620
AatII G_ACGT'C
Cuts at: 3553 3553
Size: 10760
AccI GT'mk_AC
Cuts at: 190 1375 5139 7013 7798 190
Size: 1185 3764 1874 785 3152
Fragments arranged by size:
3764 3152 1874 1185 785
Acc65I G'GTAC_C
Cuts at: 7776 7776
Size: 10760
AclI AA'CG_TT
Cuts at: 10600 10600
Size: 10760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 409 931 1295 1345 1577 2203 3155 3942
Size: 246 522 364 50 232 626 952 787
Cuts at: 3942 5312 6205 6940 7007 7123 7477 7712 8585
Size: 1370 893 735 67 116 354 235 873
Cuts at: 8585 9017 9764 10032 163
Size: 432 747 268 891
Fragments arranged by size:
1370 952 893 891 873 787 747 735
626 522 432 364 354 268 246 235
232 116 67 50
AfeI AGC'GCT
Cuts at: 3069 6075 3069
Size: 3006 7754
AflII C'TTAA_G
Cuts at: 558 2694 6511 558
Size: 2136 3817 4807
Fragments arranged by size:
4807 3817 2136
AflIII A'CryG_T
Cuts at: 4283 8112 9216 10193 4283
Size: 3829 1104 977 4850
Fragments arranged by size:
4850 3829 1104 977
AgeI A'CCGG_T
Cuts at: 9148 9148
Size: 10760
AhdI GACnn_n'nnGTC
Cuts at: 1395 1395
Size: 10760
AleI CACnn'nnGTG
Cuts at: 4615 4615
Size: 10760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3695 7436 3695
Size: 3741 7019
AlwNI CAG_nnn'CTG
Cuts at: 2034 2500 2793 2874 2976 3396 3441 3559 3711
Size: 466 293 81 102 420 45 118 152
Cuts at: 3711 3924 4153 5437 5892 6594 8065 9632 2034
Size: 213 229 1284 455 702 1471 1567 3162
Fragments arranged by size:
3162 1567 1471 1284 702 466 455 420
293 229 213 152 118 102 81 45
ApaI G_GGCC'C
Cuts at: 872 4983 5776 6561 872
Size: 4111 793 785 5071
Fragments arranged by size:
5071 4111 793 785
ApaLI G'TGCA_C
Cuts at: 4182 7196 9530 4182
Size: 3014 2334 5412
Fragments arranged by size:
5412 3014 2334
ApoI r'AATT_y
Cuts at: 1509 7764 10113 10369 1509
Size: 6255 2349 256 1900
Fragments arranged by size:
6255 2349 1900 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10760
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10760
AvaI C'yCGr_G
Cuts at: 743 2211 3338 3488 3905 3929 4976 4997 5007
Size: 1468 1127 150 417 24 1047 21 10
Cuts at: 5007 5285 6554 7109 7780 743
Size: 278 1269 555 671 3723
Fragments arranged by size:
3723 1468 1269 1127 1047 671 555 417
278 150 24 21 10
BamHI G'GATC_C
Cuts at: 230 7785 230
Size: 7555 3205
BanI G'GyrC_C
Cuts at: 2295 3208 4194 5929 6046 7255 7776 8190 8438
Size: 913 986 1735 117 1209 521 414 248
Cuts at: 8438 8473 2295
Size: 35 4582
Fragments arranged by size:
4582 1735 1209 986 913 521 414 248
117 35
BanII G_rGCy'C
Cuts at: 272 872 2024 3424 4023 4517 4919 4983 5776
Size: 600 1152 1400 599 494 402 64 793
Cuts at: 5776 5975 6561 6834 6974 7052 7774 8804 272
Size: 199 586 273 140 78 722 1030 2228
Fragments arranged by size:
2228 1400 1152 1030 793 722 600 599
586 494 402 273 199 140 78 64
BbeI G_GCGC'C
Cuts at: 5933 8194 8442 5933
Size: 2261 248 8251
Fragments arranged by size:
8251 2261 248
BbsI GAAGACnn'nnnn_
Cuts at: 175 424 1159 4049 6193 6650 175
Size: 249 735 2890 2144 457 4285
Fragments arranged by size:
4285 2890 2144 735 457 249
BbvCI CC'TCA_GC
Cuts at: 2657 2807 2904 6338 2657
Size: 150 97 3434 7079
Fragments arranged by size:
7079 3434 150 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3886 3920 4426 4460 3886
Size: 34 506 34 10186
Fragments arranged by size:
10186 506 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8658 9419 10760 8658
Size: 761 1341 8658
Fragments arranged by size:
8658 1341 761
BclI T'GATC_A
Cuts at: 8280 8280
Size: 10760
BglI GCCn_nnn'nGGC
Cuts at: 1603 1645 1711 2389 2497 2647 2893 3244 3688
Size: 42 66 678 108 150 246 351 444
Cuts at: 3688 3737 3766 4417 5467 5821 6565 7075 1603
Size: 49 29 651 1050 354 744 510 5288
Fragments arranged by size:
5288 1050 744 678 651 510 444 354
351 246 150 108 66 49 42 29
BglII A'GATC_T
Cuts at: 959 3312 5249 8275 10312 959
Size: 2353 1937 3026 2037 1407
Fragments arranged by size:
3026 2353 2037 1937 1407
BlpI GC'TnA_GC
Cuts at: 2018 2930 3071 3423 7872 2018
Size: 912 141 352 4449 4906
Fragments arranged by size:
4906 4449 912 352 141
Bme1580I G_kGCm'C
Cuts at: 477 872 1106 2004 2256 3211 4186 4794 4983
Size: 395 234 898 252 955 975 608 189
Cuts at: 4983 5776 5988 6561 7200 8385 8478 9534 477
Size: 793 212 573 639 1185 93 1056 1703
Fragments arranged by size:
1703 1185 1056 975 955 898 793 639
608 573 395 252 234 212 189 93
BmgBI CAC'GTC
Cuts at: 6015 6015
Size: 10760
BmrI ACTGGGnnnn_n'
Cuts at: 3451 3830 4258 4788 5942 6264 7972 8053 8388
Size: 379 428 530 1154 322 1708 81 335
Cuts at: 8388 10532 3451
Size: 2144 3679
Fragments arranged by size:
3679 2144 1708 1154 530 428 379 335
322 81
BmtI G_CTAG'C
Cuts at: 1030 1030
Size: 10760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1476 1773 5220 6024 9127 1476
Size: 297 3447 804 3103 3109
Fragments arranged by size:
3447 3109 3103 804 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1405 1511 1611 1939 2695 2896 4276 4843
Size: 1074 106 100 328 756 201 1380 567
Cuts at: 4843 4854 5075 5122 5533 6013 6583 331
Size: 11 221 47 411 480 570 4508
Fragments arranged by size:
4508 1380 1074 756 570 567 480 411
328 221 201 106 100 47 11
Bpu10I CC'TnA_GC
Cuts at: 2657 2807 2904 3248 5383 6338 2657
Size: 150 97 344 2135 955 7079
Fragments arranged by size:
7079 2135 955 344 150 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 2161 4102 5192 7936 8815 9307 9605 1024
Size: 1137 1941 1090 2744 879 492 298 2179
Fragments arranged by size:
2744 2179 1941 1137 1090 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 375 6012 6343 6395 10464 375
Size: 5637 331 52 4069 671
Fragments arranged by size:
5637 4069 671 331 52
BsaAI yAC'GTr
Cuts at: 1020 7752 8113 1020
Size: 6732 361 3667
Fragments arranged by size:
6732 3667 361
BsaBI GATnn'nnATC
Cuts at: 6 8274 10311 6
Size: 8268 2037 455
Fragments arranged by size:
8268 2037 455
BsaHI Gr'CG_yC
Cuts at: 206 3032 3550 5930 8191 8439 9141 10637 206
Size: 2826 518 2380 2261 248 702 1496 329
Fragments arranged by size:
2826 2380 2261 1496 702 518 329 248
BsaWI w'CCGG_w
Cuts at: 3227 7949 8470 9148 9422 9569 9980 3227
Size: 4722 521 678 274 147 411 4007
Fragments arranged by size:
4722 4007 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 799 829 1039 1069 799
Size: 30 210 30 10490
Fragments arranged by size:
10490 210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 1384 1574 1857 2791 3640 4795 5530 5779
Size: 687 190 283 934 849 1155 735 249
Cuts at: 5779 7165 697
Size: 1386 4292
Fragments arranged by size:
4292 1386 1155 934 849 735 687 283
249 190
BseYI C'CCAG_C
Cuts at: 243 256 316 1199 1538 1598 2047 2195 2500
Size: 13 60 883 339 60 449 148 305
Cuts at: 2500 2581 2969 3050 3064 3434 3865 4187 4332
Size: 81 388 81 14 370 431 322 145
Cuts at: 4332 5300 5304 5587 5769 5892 6034 6238 6315
Size: 968 4 283 182 123 142 204 77
Cuts at: 6315 7433 8185 8943 9520 10016 243
Size: 1118 752 758 577 496 987
Fragments arranged by size:
1118 987 968 883 758 752 577 496
449 431 388 370 339 322 305 283
204 182 148 145 142 123 81 81
77 60 60 14 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1663 1871 2887 3004 3046 3266 3527 3586
Size: 867 208 1016 117 42 220 261 59
Cuts at: 3586 3916 4430 4619 5545 5961 6341 6820 796
Size: 330 514 189 926 416 380 479 4736
Fragments arranged by size:
4736 1016 926 867 514 479 416 380
330 261 220 208 189 117 59 42
BsiEI CG_ry'CG
Cuts at: 104 8302 8348 9556 104
Size: 8198 46 1208 1308
Fragments arranged by size:
8198 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 689 3424 4186 4601 5975 6974 7052 7200 7748
Size: 2735 762 415 1374 999 78 148 548
Cuts at: 7748 7774 8552 8742 9534 689
Size: 26 778 190 792 1915
Fragments arranged by size:
2735 1915 1374 999 792 778 762 548
415 190 148 78 26
BsmI GAATG_Cn'
Cuts at: 2850 4874 2850
Size: 2024 8736
BsmBI CGTCTCn'nnnn_
Cuts at: 685 1214 4231 685
Size: 529 3017 7214
Fragments arranged by size:
7214 3017 529
Bsp1286I G_dGCh'C
Cuts at: 272 477 689 872 1106 2004 2024 2256 3211
Size: 205 212 183 234 898 20 232 955
Cuts at: 3211 3424 4023 4186 4517 4601 4794 4919 4983
Size: 213 599 163 331 84 193 125 64
Cuts at: 4983 5776 5975 5988 6561 6834 6974 7052 7200
Size: 793 199 13 573 273 140 78 148
Cuts at: 7200 7748 7774 8385 8478 8552 8742 8804 9534
Size: 548 26 611 93 74 190 62 730
Cuts at: 9534 272
Size: 1498
Fragments arranged by size:
1498 955 898 793 730 611 599 573
548 331 273 234 232 213 212 205
199 193 190 183 163 148 140 125
93 84 78 74 64 62 26 20
13
BspEI T'CCGG_A
Cuts at: 3227 9980 3227
Size: 6753 4007
BspHI T'CATG_A
Cuts at: 461 495 9936 461
Size: 34 9441 1285
Fragments arranged by size:
9441 1285 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2042 3466 3490 4388 6630 7404 7810 8024 8326
Size: 1424 24 898 2242 774 406 214 302
Cuts at: 8326 8707 2042
Size: 381 4095
Fragments arranged by size:
4095 2242 1424 898 774 406 381 302
214 24
BsrBI CCG'CTC
Cuts at: 50 228 5346 5911 9052 9106 10590 10755 50
Size: 178 5118 565 3141 54 1484 165 55
Fragments arranged by size:
5118 3141 1484 565 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 574 1298 3083 3468 5117 5714 6089 6505 6728
Size: 724 1785 385 1649 597 375 416 223
Cuts at: 6728 7034 7178 7487 7616 8672 574
Size: 306 144 309 129 1056 2662
Fragments arranged by size:
2662 1785 1649 1056 724 597 416 385
375 309 306 223 144 129
BsrFI r'CCGG_y
Cuts at: 1303 2962 3845 5026 8758 8939 9148 1303
Size: 1659 883 1181 3732 181 209 2915
Fragments arranged by size:
3732 2915 1659 1181 883 209 181
BsrGI T'GTAC_A
Cuts at: 753 4701 7368 753
Size: 3948 2667 4145
Fragments arranged by size:
4145 3948 2667
BssSI C'ACGA_G
Cuts at: 688 782 9031 9389 688
Size: 94 8249 358 2059
Fragments arranged by size:
8249 2059 358 94
BstAPI GCAn_nnn'nTGC
Cuts at: 618 1641 2158 2415 2793 3481 4453 5365 5437
Size: 1023 517 257 378 688 972 912 72
Cuts at: 5437 5581 7360 8137 618
Size: 144 1779 777 3241
Fragments arranged by size:
3241 1779 1023 972 912 777 688 517
378 257 144 72
BstBI TT'CG_AA
Cuts at: 110 9121 110
Size: 9011 1749
BstEII G'GTnAC_C
Cuts at: 5476 5739 5916 6489 5476
Size: 263 177 573 9747
Fragments arranged by size:
9747 573 263 177
BstXI CCAn_nnnn'nTGG
Cuts at: 1113 2068 2148 2190 2364 3204 3891 4617 4719
Size: 955 80 42 174 840 687 726 102
Cuts at: 4719 6222 6396 6486 7200 7275 1113
Size: 1503 174 90 714 75 4598
Fragments arranged by size:
4598 1503 955 840 726 714 687 174
174 102 90 80 75 42
BstYI r'GATC_y
Cuts at: 1 230 392 857 959 1751 2708 3312 3555
Size: 229 162 465 102 792 957 604 243
Cuts at: 3555 5249 7697 7785 8252 8275 8610 8856 9868
Size: 1694 2448 88 467 23 335 246 1012
Cuts at: 9868 9954 9966 10312 1
Size: 86 12 346 449
Fragments arranged by size:
2448 1694 1012 957 792 604 467 465
449 346 335 246 243 229 162 102
88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3763 3917 3936 4895 3763
Size: 154 19 959 9628
Fragments arranged by size:
9628 959 154 19
BtgI C'CryG_G
Cuts at: 125 194 651 1890 2363 2570 4094 4910 5898
Size: 69 457 1239 473 207 1524 816 988
Cuts at: 5898 6278 6527 6674 125
Size: 380 249 147 4211
Fragments arranged by size:
4211 1524 1239 988 816 473 457 380
249 207 147 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2904 3414 3761 7614 8710 8891 9054 2904
Size: 510 347 3853 1096 181 163 4610
Fragments arranged by size:
4610 3853 1096 510 347 181 163
BtsI GCAGTG_nn'
Cuts at: 1847 1941 1985 2004 3005 3016 3569 3704 5234
Size: 94 44 19 1001 11 553 135 1530
Cuts at: 5234 5366 5456 5726 5944 6122 6380 7294 7517
Size: 132 90 270 218 178 258 914 223
Cuts at: 7517 8003 1847
Size: 486 4604
Fragments arranged by size:
4604 1530 1001 914 553 486 270 258
223 218 178 135 132 94 90 44
19 11
ClaI AT'CG_AT
Cuts at: 1313 1313
Size: 10760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 963 998 4506 4541 6932 6967 7017 7052 963
Size: 35 3508 35 2391 35 50 35 4671
Fragments arranged by size:
4671 3508 2391 50 35 35 35 35
DraI TTT'AAA
Cuts at: 7759 7759
Size: 10760
DraIII CAC_nnn'GTG
Cuts at: 2251 3289 4181 4426 5316 2251
Size: 1038 892 245 890 7695
Fragments arranged by size:
7695 1038 892 890 245
DrdI GACnn_nn'nnGTC
Cuts at: 1884 6217 7030 8466 9324 1884
Size: 4333 813 1436 858 3320
Fragments arranged by size:
4333 3320 1436 858 813
EaeI y'GGCC_r
Cuts at: 1557 1692 2511 2822 3587 3645 3738 4200 4721
Size: 135 819 311 765 58 93 462 521
Cuts at: 4721 5024 5028 5114 5273 5805 6843 7331 8345
Size: 303 4 86 159 532 1038 488 1014
Cuts at: 8345 8519 8910 8937 10282 1557
Size: 174 391 27 1345 2035
Fragments arranged by size:
2035 1345 1038 1014 819 765 532 521
488 462 391 311 303 174 159 135
93 86 58 27 4
EagI C'GGCC_G
Cuts at: 8345 8345
Size: 10760
EarI CTCTTCn'nnn_
Cuts at: 524 5849 7562 7667 8783 8993 524
Size: 5325 1713 105 1116 210 2291
Fragments arranged by size:
5325 2291 1713 1116 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8968 9278 9424 10570 8968
Size: 310 146 1146 9158
Fragments arranged by size:
9158 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3422 5973 6972 7050 7772 3422
Size: 2551 999 78 722 6410
Fragments arranged by size:
6410 2551 999 722 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 331 409 931 1295 1345 1405 1511 1577
Size: 168 78 522 364 50 60 106 66
Cuts at: 1577 1611 1939 2203 2695 2896 3155 3942 4276
Size: 34 328 264 492 201 259 787 334
Cuts at: 4276 4843 4854 5075 5122 5312 5533 6013 6205
Size: 567 11 221 47 190 221 480 192
Cuts at: 6205 6583 6940 7007 7123 7477 7712 8585 9017
Size: 378 357 67 116 354 235 873 432
Cuts at: 9017 9764 10032 163
Size: 747 268 891
Fragments arranged by size:
891 873 787 747 567 522 492 480
432 378 364 357 354 334 328 268
264 259 235 221 221 201 192 190
168 116 106 78 67 66 60 50
47 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 925 1069 1951 3622 5152 5515 5569 925
Size: 144 882 1671 1530 363 54 6116
Fragments arranged by size:
6116 1671 1530 882 363 144 54
EcoO109I rG'GnC_Cy
Cuts at: 240 554 747 869 1815 1965 2454 3393 3657
Size: 314 193 122 946 150 489 939 264
Cuts at: 3657 3921 4086 4769 4980 5001 5145 5550 5773
Size: 264 165 683 211 21 144 405 223
Cuts at: 5773 6219 6342 6558 7023 7299 7899 240
Size: 446 123 216 465 276 600 3101
Fragments arranged by size:
3101 946 939 683 600 489 465 446
405 314 276 264 264 223 216 211
193 165 150 144 123 122 21
EcoRI G'AATT_C
Cuts at: 7764 7764
Size: 10760
EcoRV GAT'ATC
Cuts at: 1317 1317
Size: 10760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 915 1365 7666 8779 915
Size: 450 6301 1113 2896
Fragments arranged by size:
6301 2896 1113 450
FseI GG_CCGG'CC
Cuts at: 5030 5030
Size: 10760
FspI TGC'GCA
Cuts at: 1996 2818 2840 8541 1996
Size: 822 22 5701 4215
Fragments arranged by size:
5701 4215 822 22
HaeII r_GCGC'y
Cuts at: 2874 3071 4695 4762 5933 6077 8194 8442 9464
Size: 197 1624 67 1171 144 2117 248 1022
Cuts at: 9464 10443 2874
Size: 979 3191
Fragments arranged by size:
3191 2117 1624 1171 1022 979 248 197
144 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 776 808 1357 1381 1389 1413 1690 1722 1743
Size: 32 549 24 8 24 277 32 21
Cuts at: 1743 1775 3540 3572 3693 3725 5241 5273 6392
Size: 32 1765 32 121 32 1516 32 1119
Cuts at: 6392 6424 7456 7488 8602 8632 8634 8664 776
Size: 32 1032 32 1114 30 2 30 2872
Fragments arranged by size:
2872 1765 1516 1119 1114 1032 549 277
121 32 32 32 32 32 32 32
32 30 30 24 24 21 8 2
HincII GTy'rAC
Cuts at: 1412 6932 7799 1412
Size: 5520 867 4373
Fragments arranged by size:
5520 4373 867
HindIII A'AGCT_T
Cuts at: 323 1218 3349 323
Size: 895 2131 7734
Fragments arranged by size:
7734 2131 895
KasI G'GCGC_C
Cuts at: 5929 8190 8438 5929
Size: 2261 248 8251
Fragments arranged by size:
8251 2261 248
KpnI G_GTAC'C
Cuts at: 7780 7780
Size: 10760
MluI A'CGCG_T
Cuts at: 10193 10193
Size: 10760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 637 1895 3753 4058 4880 6420 9431 9615 637
Size: 1258 1858 305 822 1540 3011 184 1782
Fragments arranged by size:
3011 1858 1782 1540 1258 822 305 184
MscI TGG'CCA
Cuts at: 1694 2824 3647 3740 4723 5275 8521 1694
Size: 1130 823 93 983 552 3246 3933
Fragments arranged by size:
3933 3246 1130 983 823 552 93
MslI CAynn'nnrTG
Cuts at: 454 460 626 4051 4177 4615 5227 5719 5903
Size: 6 166 3425 126 438 612 492 184
Cuts at: 5903 6112 6655 8749 8876 454
Size: 209 543 2094 127 2338
Fragments arranged by size:
3425 2338 2094 612 543 492 438 209
184 166 127 126 6
MspA1I CmG'CkG
Cuts at: 247 759 1556 1780 2047 2164 2199 2443 2758
Size: 512 797 224 267 117 35 244 315
Cuts at: 2758 2790 2806 2836 3133 3183 3643 3675 3719
Size: 32 16 30 297 50 460 32 44
Cuts at: 3719 3728 4128 4138 4444 4649 5100 5271 5304
Size: 9 400 10 306 205 451 171 33
Cuts at: 5304 5790 5836 6238 6430 7101 7366 7871 8185
Size: 486 46 402 192 671 265 505 314
Cuts at: 8185 8545 9558 9803 247
Size: 360 1013 245 1204
Fragments arranged by size:
1204 1013 797 671 512 505 486 460
451 402 400 360 315 314 306 297
267 265 245 244 224 205 192 171
117 50 46 44 35 33 32 32
30 16 10 9
NaeI GCC'GGC
Cuts at: 1305 2964 5028 8941 1305
Size: 1659 2064 3913 3124
Fragments arranged by size:
3913 3124 2064 1659
NarI GG'CG_CC
Cuts at: 5930 8191 8439 5930
Size: 2261 248 8251
Fragments arranged by size:
8251 2261 248
NcoI C'CATG_G
Cuts at: 125 194 651 2570 4910 5898 6674 125
Size: 69 457 1919 2340 988 776 4211
Fragments arranged by size:
4211 2340 1919 988 776 457 69
NgoMIV G'CCGG_C
Cuts at: 1303 2962 5026 8939 1303
Size: 1659 2064 3913 3124
Fragments arranged by size:
3913 3124 2064 1659
NheI G'CTAG_C
Cuts at: 1026 1026
Size: 10760
NspI r_CATG'y
Cuts at: 342 459 1530 1581 1635 2479 2884 3260 4287
Size: 117 1071 51 54 844 405 376 1027
Cuts at: 4287 7813 8313 9220 342
Size: 3526 500 907 1882
Fragments arranged by size:
3526 1882 1071 1027 907 844 500 405
376 117 54 51
PasI CC'CwG_GG
Cuts at: 3456 6396 7296 3456
Size: 2940 900 6920
Fragments arranged by size:
6920 2940 900
PciI A'CATG_T
Cuts at: 4283 9216 4283
Size: 4933 5827
PflMI CCAn_nnn'nTGG
Cuts at: 735 2274 3396 3441 4390 4566 6018 735
Size: 1539 1122 45 949 176 1452 5477
Fragments arranged by size:
5477 1539 1452 1122 949 176 45
PfoI T'CCnGG_A
Cuts at: 1169 2117 3968 4244 4253 5408 1169
Size: 948 1851 276 9 1155 6521
Fragments arranged by size:
6521 1851 1155 948 276 9
PmeI GTTT'AAAC
Cuts at: 7759 7759
Size: 10760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9899 9931 9899
Size: 32 10728
PpuMI rG'GwC_Cy
Cuts at: 554 747 1815 3393 3657 3921 5001 5145 5550
Size: 193 1068 1578 264 264 1080 144 405
Cuts at: 5550 6219 6342 7023 554
Size: 669 123 681 4291
Fragments arranged by size:
4291 1578 1080 1068 681 669 405 264
264 193 144 123
PshAI GACnn'nnGTC
Cuts at: 653 653
Size: 10760
PspOMI G'GGCC_C
Cuts at: 868 4979 5772 6557 868
Size: 4111 793 785 5071
Fragments arranged by size:
5071 4111 793 785
PspXI vC'TCGA_Gb
Cuts at: 3929 3929
Size: 10760
PstI C_TGCA'G
Cuts at: 1424 2163 2241 2474 2901 2928 3110 3564 4137
Size: 739 78 233 427 27 182 454 573
Cuts at: 4137 4608 5172 5241 5361 5447 5835 5939 6429
Size: 471 564 69 120 86 388 104 490
Cuts at: 6429 6902 7065 7083 7560 7807 8492 1424
Size: 473 163 18 477 247 685 3692
Fragments arranged by size:
3692 739 685 573 564 490 477 473
471 454 427 388 247 233 182 163
120 104 86 78 69 27 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10760
PvuII CAG'CTG
Cuts at: 247 759 1780 2047 2164 2443 2758 2806 2836
Size: 512 1021 267 117 279 315 48 30
Cuts at: 2836 3133 3183 3643 3728 4128 4138 4444 4649
Size: 297 50 460 85 400 10 306 205
Cuts at: 4649 5100 5304 5790 6238 6430 7101 7366 8185
Size: 451 204 486 448 192 671 265 819
Cuts at: 8185 8545 247
Size: 360 2462
Fragments arranged by size:
2462 1021 819 671 512 486 460 451
448 400 360 315 306 297 279 267
265 205 204 192 117 85 50 48
30 10
RsrII CG'GwC_CG
Cuts at: 4242 8955 4242
Size: 4713 6047
SacI G_AGCT'C
Cuts at: 3424 5975 6974 7052 7774 3424
Size: 2551 999 78 722 6410
Fragments arranged by size:
6410 2551 999 722 78
SalI G'TCGA_C
Cuts at: 7797 7797
Size: 10760
SanDI GG'GwC_CC
Cuts at: 747 1815 3657 5001 747
Size: 1068 1842 1344 6506
Fragments arranged by size:
6506 1842 1344 1068
SapI GCTCTTCn'nnn_
Cuts at: 7562 8783 8993 7562
Size: 1221 210 9329
Fragments arranged by size:
9329 1221 210
SbfI CC_TGCA'GG
Cuts at: 7807 7807
Size: 10760
ScaI AGT'ACT
Cuts at: 5335 10277 5335
Size: 4942 5818
SexAI A'CCwGG_T
Cuts at: 2630 2630
Size: 10760
SfcI C'TryA_G
Cuts at: 33 1420 2159 2237 2396 2470 2897 2924 3106
Size: 1387 739 78 159 74 427 27 182
Cuts at: 3106 3316 3560 4133 4604 5168 5237 5357 5443
Size: 210 244 573 471 564 69 120 86
Cuts at: 5443 5831 5935 6425 6898 7061 7079 7556 7803
Size: 388 104 490 473 163 18 477 247
Cuts at: 7803 8488 9481 9672 33
Size: 685 993 191 1121
Fragments arranged by size:
1387 1121 993 739 685 573 564 490
477 473 471 427 388 247 244 210
191 182 163 159 120 104 86 78
74 69 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 5821 5821
Size: 10760
SfoI GGC'GCC
Cuts at: 5931 8192 8440 5931
Size: 2261 248 8251
Fragments arranged by size:
8251 2261 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10760
SmaI CCC'GGG
Cuts at: 745 4978 4999 5287 7111 7782 745
Size: 4233 21 288 1824 671 3723
Fragments arranged by size:
4233 3723 1824 671 288 21
SmlI C'TyrA_G
Cuts at: 558 1039 2176 2694 3338 3929 4117 5207 6511
Size: 481 1137 518 644 591 188 1090 1304
Cuts at: 6511 7915 8830 9322 9584 558
Size: 1404 915 492 262 1734
Fragments arranged by size:
1734 1404 1304 1137 1090 915 644 591
518 492 481 262 188
SnaBI TAC'GTA
Cuts at: 7752 7752
Size: 10760
SpeI A'CTAG_T
Cuts at: 67 2271 7233 67
Size: 2204 4962 3594
Fragments arranged by size:
4962 3594 2204
SphI G_CATG'C
Cuts at: 1530 1635 2884 7813 8313 1530
Size: 105 1249 4929 500 3977
Fragments arranged by size:
4929 3977 1249 500 105
SrfI GCCC'GGGC
Cuts at: 4978 5287 4978
Size: 309 10451
SspI AAT'ATT
Cuts at: 505 505
Size: 10760
StuI AGG'CCT
Cuts at: 829 1714 2956 3142 3901 5215 6055 829
Size: 885 1242 186 759 1314 840 5534
Fragments arranged by size:
5534 1314 1242 885 840 759 186
StyI C'CwwG_G
Cuts at: 125 194 651 926 1007 1124 1157 2570 2747
Size: 69 457 275 81 117 33 1413 177
Cuts at: 2747 2951 4082 4712 4910 5462 5708 5898 5921
Size: 204 1131 630 198 552 246 190 23
Cuts at: 5921 6215 6479 6662 6674 6686 7121 7242 7894
Size: 294 264 183 12 12 435 121 652
Cuts at: 7894 8248 125
Size: 354 2637
Fragments arranged by size:
2637 1413 1131 652 630 552 457 435
354 294 275 264 246 204 198 190
183 177 121 117 81 69 33 23
12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2464 2563 4031 4201 5353 5515 5551 5587 5635
Size: 99 1468 170 1152 162 36 36 48
Cuts at: 5635 5644 6064 6307 6815 7243 8343 8476 8935
Size: 9 420 243 508 428 1100 133 459
Cuts at: 8935 9144 2464
Size: 209 4080
Fragments arranged by size:
4080 1468 1152 1100 508 459 428 420
243 209 170 162 133 99 48 36
36 9
TatI w'GTAC_w
Cuts at: 365 753 1263 2281 3576 4701 5333 6885 7368
Size: 388 510 1018 1295 1125 632 1552 483
Cuts at: 7368 7712 10275 365
Size: 344 2563 850
Fragments arranged by size:
2563 1552 1295 1125 1018 850 632 510
483 388 344
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 799 831 5300 5302 5332 5334 7239 7271 10277
Size: 32 4469 2 30 2 1905 32 3006
Cuts at: 10277 10309 799
Size: 32 1250
Fragments arranged by size:
4469 3006 1905 1250 32 32 32 30
2 2
Tth111I GACn'n_nGTC
Cuts at: 2632 8557 2632
Size: 5925 4835
XbaI T'CTAG_A
Cuts at: 3303 7791 3303
Size: 4488 6272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 862 2628 2730 3597 6864 7197 8255 862
Size: 1766 102 867 3267 333 1058 3367
Fragments arranged by size:
3367 3267 1766 1058 867 333 102
XhoI C'TCGA_G
Cuts at: 3338 3929 3338
Size: 591 10169
XmaI C'CCGG_G
Cuts at: 743 4976 4997 5285 7109 7780 743
Size: 4233 21 288 1824 671 3723
Fragments arranged by size:
4233 3723 1824 671 288 21
XmnI GAAnn'nnTTC
Cuts at: 916 7649 916
Size: 6733 4027
ZraI GAC'GTC
Cuts at: 3551 3551
Size: 10760
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BssHII BstZ17I
FspAI HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PsiI PsrI SacII SgrAI SwaI