Restriction map for
pF1KSDA1034
(Circular) (Six-base) MAPSORT of: pF1KSDA1034.seq Check: 9843 from: 1 to: 5338
pF1KSDA1034 5338 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2313 2313
Size: 5338
AatII G_ACGT'C
Cuts at: 194 194
Size: 5338
AccI GT'mk_AC
Cuts at: 2374 2374
Size: 5338
Acc65I G'GTAC_C
Cuts at: 2126 2352 2126
Size: 226 5112
AclI AA'CG_TT
Cuts at: 5178 5178
Size: 5338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1967 3161 3593 4342 4610 1261
Size: 706 1194 432 749 268 1989
Fragments arranged by size:
1989 1194 749 706 432 268
AflIII A'CryG_T
Cuts at: 1833 2688 3794 4771 1833
Size: 855 1106 977 2400
Fragments arranged by size:
2400 1106 977 855
AgeI A'CCGG_T
Cuts at: 3726 3726
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 795 795
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 1143 2305 2641 4210 1143
Size: 1162 336 1569 2271
Fragments arranged by size:
2271 1569 1162 336
ApaLI G'TGCA_C
Cuts at: 4108 4108
Size: 5338
ApoI r'AATT_y
Cuts at: 950 1383 2340 4691 4947 950
Size: 433 957 2351 256 1341
Fragments arranged by size:
2351 1341 957 433 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5338
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5338
AvaI C'yCGr_G
Cuts at: 2356 2356
Size: 5338
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1725 1758 1725
Size: 33 5305
BamHI G'GATC_C
Cuts at: 2006 2361 2006
Size: 355 4983
BanI G'GyrC_C
Cuts at: 271 1558 2126 2352 2766 3014 3049 271
Size: 1287 568 226 414 248 35 2560
Fragments arranged by size:
2560 1287 568 414 248 226 35
BanII G_rGCy'C
Cuts at: 148 255 1009 2170 2350 3380 148
Size: 107 754 1161 180 1030 2106
Fragments arranged by size:
2106 1161 1030 754 180 107
BbeI G_GCGC'C
Cuts at: 275 1562 2770 3018 275
Size: 1287 1208 248 2595
Fragments arranged by size:
2595 1287 1208 248
BbsI GAAGACnn'nnnn_
Cuts at: 620 1006 1337 1955 620
Size: 386 331 618 4003
Fragments arranged by size:
4003 618 386 331
BbvCI CC'TCA_GC
Cuts at: 2270 2270
Size: 5338
BciVI GTATCCnnnnn_n'
Cuts at: 3234 3997 5338 3234
Size: 763 1341 3234
Fragments arranged by size:
3234 1341 763
BclI T'GATC_A
Cuts at: 2856 2856
Size: 5338
BfrBI ATG'CAT
Cuts at: 885 885
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 1648 1648
Size: 5338
BglII A'GATC_T
Cuts at: 1985 2851 4890 1985
Size: 866 2039 2433
Fragments arranged by size:
2433 2039 866
BlpI GC'TnA_GC
Cuts at: 2075 2448 2075
Size: 373 4965
Bme1580I G_kGCm'C
Cuts at: 986 2961 3054 4112 986
Size: 1975 93 1058 2212
Fragments arranged by size:
2212 1975 1058 93
BmgBI CAC'GTC
Cuts at: 2181 2181
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 202 2548 2629 2964 5110 202
Size: 2346 81 335 2146 430
Fragments arranged by size:
2346 2146 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1925 2259 3703 1925
Size: 334 1444 3560
Fragments arranged by size:
3560 1444 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1193 1464 247
Size: 946 271 4121
Fragments arranged by size:
4121 946 271
Bpu10I CC'TnA_GC
Cuts at: 1853 2270 1853
Size: 417 4921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1613 2512 3391 3885 4183 1106
Size: 507 899 879 494 298 2261
Fragments arranged by size:
2261 899 879 507 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1473 1979 5042 1473
Size: 506 3063 1769
Fragments arranged by size:
3063 1769 506
BsaAI yAC'GTr
Cuts at: 1836 2133 2328 2689 1836
Size: 297 195 361 4485
Fragments arranged by size:
4485 361 297 195
BsaBI GATnn'nnATC
Cuts at: 6 563 2850 4889 6
Size: 557 2287 2039 455
Fragments arranged by size:
2287 2039 557 455
BsaHI Gr'CG_yC
Cuts at: 191 272 1559 2767 3015 3719 5215 191
Size: 81 1287 1208 248 704 1496 314
Fragments arranged by size:
1496 1287 1208 704 314 248 81
BsaWI w'CCGG_w
Cuts at: 2525 3046 3726 4000 4147 4558 2525
Size: 521 680 274 147 411 3305
Fragments arranged by size:
3305 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2152 2182 2152
Size: 30 5308
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 929 1461 1485 1810 1813 1928 2215 2236 2263
Size: 532 24 325 3 115 287 21 27
Cuts at: 2263 2287 929
Size: 24 3980
Fragments arranged by size:
3980 532 325 287 115 27 24 24
21 3
BseYI C'CCAG_C
Cuts at: 483 1025 1489 1769 2761 3519 4098 4594 483
Size: 542 464 280 992 758 579 496 1227
Fragments arranged by size:
1227 992 758 579 542 496 464 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 1882
Size: 5338
BsiEI CG_ry'CG
Cuts at: 104 171 2878 2924 4134 104
Size: 67 2707 46 1210 1308
Fragments arranged by size:
2707 1308 1210 67 46
BsiHKAI G_wGCw'C
Cuts at: 694 2162 2350 3128 3318 4112 694
Size: 1468 188 778 190 794 1920
Fragments arranged by size:
1920 1468 794 778 190 188
BsmI GAATG_Cn'
Cuts at: 712 1282 712
Size: 570 4768
Bsp1286I G_dGCh'C
Cuts at: 148 255 694 986 1009 2162 2170 2350 2961
Size: 107 439 292 23 1153 8 180 611
Cuts at: 2961 3054 3128 3318 3380 4112 148
Size: 93 74 190 62 732 1374
Fragments arranged by size:
1374 1153 732 611 439 292 190 180
107 93 74 62 23 8
BspEI T'CCGG_A
Cuts at: 4558 4558
Size: 5338
BspHI T'CATG_A
Cuts at: 1001 2025 4514 1001
Size: 1024 2489 1825
Fragments arranged by size:
2489 1825 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1854 2313 2386 2600 2902 3283 1854
Size: 459 73 214 302 381 3909
Fragments arranged by size:
3909 459 381 302 214 73
BsrBI CCG'CTC
Cuts at: 50 132 179 1440 3628 3682 5168 5333 50
Size: 82 47 1261 2188 54 1486 165 55
Fragments arranged by size:
2188 1486 1261 165 82 55 54 47
BsrDI GCAATG_nn'
Cuts at: 660 3248 660
Size: 2588 2750
BsrFI r'CCGG_y
Cuts at: 170 1097 1857 3334 3515 3726 170
Size: 927 760 1477 181 211 1782
Fragments arranged by size:
1782 1477 927 760 211 181
BsrGI T'GTAC_A
Cuts at: 2255 2255
Size: 5338
BssSI C'ACGA_G
Cuts at: 3607 3967 3607
Size: 360 4978
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2713 2305
Size: 408 4930
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5338
BstXI CCAn_nnnn'nTGG
Cuts at: 288 790 288
Size: 502 4836
BstYI r'GATC_y
Cuts at: 1 1595 1985 2006 2082 2361 2828 2851 3186
Size: 1594 390 21 76 279 467 23 335
Cuts at: 3186 3432 4446 4532 4544 4890 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1594 1014 467 449 390 346 335 279
246 86 76 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 1369 1508 1369
Size: 139 5199
BtgI C'CryG_G
Cuts at: 125 245 255 275 287 1061 1196 2142 2171
Size: 120 10 20 12 774 135 946 29
Cuts at: 2171 125
Size: 3292
Fragments arranged by size:
3292 946 774 135 120 29 20 12
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3286 3467 3630 3286
Size: 181 163 4994
Fragments arranged by size:
4994 181 163
BtsI GCAGTG_nn'
Cuts at: 637 2579 637
Size: 1942 3396
DraI TTT'AAA
Cuts at: 2335 2335
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 3042 3902 3042
Size: 860 4478
EaeI y'GGCC_r
Cuts at: 278 542 2921 3095 3486 3513 4860 278
Size: 264 2379 174 391 27 1347 756
Fragments arranged by size:
2379 1347 756 391 264 174 27
EagI C'GGCC_G
Cuts at: 2921 2921
Size: 5338
EarI CTCTTCn'nnn_
Cuts at: 1197 1237 1361 3359 3569 1197
Size: 40 124 1998 210 2966
Fragments arranged by size:
2966 1998 210 124 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 189 3544 3856 4002 5148 150
Size: 39 3355 312 146 1146 340
Fragments arranged by size:
3355 1146 340 312 146 39
EcoICRI GAG'CTC
Cuts at: 2348 2348
Size: 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1193 1261 1464 1967 3161 3593 4342 4610
Size: 946 68 203 503 1194 432 749 268
Cuts at: 4610 247
Size: 975
Fragments arranged by size:
1194 975 946 749 503 432 268 203
68
EcoO109I rG'GnC_Cy
Cuts at: 199 2475 199
Size: 2276 3062
EcoRI G'AATT_C
Cuts at: 2340 2340
Size: 5338
EcoRV GAT'ATC
Cuts at: 1215 1215
Size: 5338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 415 2007 3355 415
Size: 1592 1348 2398
Fragments arranged by size:
2398 1592 1348
FspI TGC'GCA
Cuts at: 3117 3117
Size: 5338
HaeII r_GCGC'y
Cuts at: 275 1562 1927 2770 3018 4042 5021 275
Size: 1287 365 843 248 1024 979 592
Fragments arranged by size:
1287 1024 979 843 592 365 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 996 1028 1332 1364 1403 1434 1435
Size: 32 829 32 304 32 39 31 1
Cuts at: 1435 1466 1786 1818 3178 3208 3210 3240 135
Size: 31 320 32 1360 30 2 30 2233
Fragments arranged by size:
2233 1360 829 320 304 39 32 32
32 32 31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 2375 2375
Size: 5338
HindIII A'AGCT_T
Cuts at: 2016 2016
Size: 5338
KasI G'GCGC_C
Cuts at: 271 1558 2766 3014 271
Size: 1287 1208 248 2595
Fragments arranged by size:
2595 1287 1208 248
KpnI G_GTAC'C
Cuts at: 2130 2356 2130
Size: 226 5112
MluI A'CGCG_T
Cuts at: 4771 4771
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 590 1623 1966 4009 4193 311
Size: 279 1033 343 2043 184 1456
Fragments arranged by size:
2043 1456 1033 343 279 184
MscI TGG'CCA
Cuts at: 280 3097 280
Size: 2817 2521
MslI CAynn'nnrTG
Cuts at: 3325 3452 3325
Size: 127 5211
MspA1I CmG'CkG
Cuts at: 257 411 906 1579 1691 2079 2124 2173 2447
Size: 154 495 673 112 388 45 49 274
Cuts at: 2447 2761 3121 4136 4381 257
Size: 314 360 1015 245 1214
Fragments arranged by size:
1214 1015 673 495 388 360 314 274
245 154 112 49 45
NaeI GCC'GGC
Cuts at: 1099 3517 1099
Size: 2418 2920
NarI GG'CG_CC
Cuts at: 272 1559 2767 3015 272
Size: 1287 1208 248 2595
Fragments arranged by size:
2595 1287 1208 248
NcoI C'CATG_G
Cuts at: 125 245 1061 125
Size: 120 816 4402
Fragments arranged by size:
4402 816 120
NgoMIV G'CCGG_C
Cuts at: 1097 3515 1097
Size: 2418 2920
NruI TCG'CGA
Cuts at: 3699 3699
Size: 5338
NsiI A_TGCA'T
Cuts at: 887 887
Size: 5338
NspI r_CATG'y
Cuts at: 552 1830 2389 2889 3798 552
Size: 1278 559 500 909 2092
Fragments arranged by size:
2092 1278 909 559 500
PasI CC'CwG_GG
Cuts at: 435 915 2001 435
Size: 480 1086 3772
Fragments arranged by size:
3772 1086 480
PciI A'CATG_T
Cuts at: 3794 3794
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2335 2335
Size: 5338
PmlI CAC'GTG
Cuts at: 1836 2133 1836
Size: 297 5041
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4477 4509 4477
Size: 32 5306
PshAI GACnn'nnGTC
Cuts at: 1881 1881
Size: 5338
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2110 2142 2110
Size: 32 5306
PstI C_TGCA'G
Cuts at: 2383 3068 2383
Size: 685 4653
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5338
PvuII CAG'CTG
Cuts at: 411 906 1579 2079 2761 3121 411
Size: 495 673 500 682 360 2628
Fragments arranged by size:
2628 682 673 500 495 360
RsrII CG'GwC_CG
Cuts at: 3531 3531
Size: 5338
SacI G_AGCT'C
Cuts at: 2350 2350
Size: 5338
SacII CC_GC'GG
Cuts at: 258 2174 258
Size: 1916 3422
SalI G'TCGA_C
Cuts at: 2373 2373
Size: 5338
SapI GCTCTTCn'nnn_
Cuts at: 3359 3569 3359
Size: 210 5128
SbfI CC_TGCA'GG
Cuts at: 2383 2383
Size: 5338
ScaI AGT'ACT
Cuts at: 963 4855 963
Size: 3892 1446
SfcI C'TryA_G
Cuts at: 33 2379 3064 4059 4250 33
Size: 2346 685 995 191 1121
Fragments arranged by size:
2346 1121 995 685 191
SfoI GGC'GCC
Cuts at: 273 1560 2768 3016 273
Size: 1287 1208 248 2595
Fragments arranged by size:
2595 1287 1208 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5338
SmaI CCC'GGG
Cuts at: 2358 2358
Size: 5338
SmlI C'TyrA_G
Cuts at: 1121 1628 2491 3406 3900 4162 1121
Size: 507 863 915 494 262 2297
Fragments arranged by size:
2297 915 863 507 494 262
SnaBI TAC'GTA
Cuts at: 2328 2328
Size: 5338
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5338
SphI G_CATG'C
Cuts at: 1830 2389 2889 1830
Size: 559 500 4279
Fragments arranged by size:
4279 559 500
StuI AGG'CCT
Cuts at: 2036 2036
Size: 5338
StyI C'CwwG_G
Cuts at: 125 245 281 468 1061 1619 1895 2470 2824
Size: 120 36 187 593 558 276 575 354
Cuts at: 2824 125
Size: 2639
Fragments arranged by size:
2639 593 575 558 354 276 187 120
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1170 2227 2919 3052 3511 3722 1170
Size: 1057 692 133 459 211 2786
Fragments arranged by size:
2786 1057 692 459 211 133
TatI w'GTAC_w
Cuts at: 809 961 2255 4853 809
Size: 152 1294 2598 1294
Fragments arranged by size:
2598 1294 1294 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 4855 4887 2152
Size: 32 2671 32 2603
Fragments arranged by size:
2671 2603 32 32
Tth111I GACn'n_nGTC
Cuts at: 2214 3133 2214
Size: 919 4419
XbaI T'CTAG_A
Cuts at: 2367 2367
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 1194 1461 2831 273
Size: 921 267 1370 2780
Fragments arranged by size:
2780 1370 921 267
XmaI C'CCGG_G
Cuts at: 2356 2356
Size: 5338
ZraI GAC'GTC
Cuts at: 192 192
Size: 5338
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BcgI BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI PacI PfoI PpuMI PsiI PspOMI PspXI
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI