Restriction map for
pF1KSDA1048
(Circular) (Six-base) MAPSORT of: pF1KSDA1048.seq Check: 1415 from: 1 to: 5726
pF1KSDA1048 5726 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2203 2764 2203
Size: 561 5165
Acc65I G'GTAC_C
Cuts at: 2742 2742
Size: 5726
AclI AA'CG_TT
Cuts at: 2180 5566 2180
Size: 3386 2340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 2177 2270 2600 3551 3983 4730 4998 676
Size: 1501 93 330 951 432 747 268 1404
Fragments arranged by size:
1501 1404 951 747 432 330 268 93
AfeI AGC'GCT
Cuts at: 1286 1286
Size: 5726
AflIII A'CryG_T
Cuts at: 3078 4182 5159 3078
Size: 1104 977 3645
Fragments arranged by size:
3645 1104 977
AgeI A'CCGG_T
Cuts at: 4114 4114
Size: 5726
AhdI GACnn_n'nnGTC
Cuts at: 259 259
Size: 5726
AlwNI CAG_nnn'CTG
Cuts at: 549 1018 1407 1482 1885 3031 4598 549
Size: 469 389 75 403 1146 1567 1677
Fragments arranged by size:
1677 1567 1146 469 403 389 75
ApaLI G'TGCA_C
Cuts at: 4496 4496
Size: 5726
ApoI r'AATT_y
Cuts at: 727 1713 2730 5079 5335 727
Size: 986 1017 2349 256 1118
Fragments arranged by size:
2349 1118 1017 986 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
AvaI C'yCGr_G
Cuts at: 207 2299 2746 207
Size: 2092 447 3187
Fragments arranged by size:
3187 2092 447
BamHI G'GATC_C
Cuts at: 1336 2751 1336
Size: 1415 4311
BanI G'GyrC_C
Cuts at: 2742 3156 3404 3439 2742
Size: 414 248 35 5029
Fragments arranged by size:
5029 414 248 35
BanII G_rGCy'C
Cuts at: 178 867 2740 3770 178
Size: 689 1873 1030 2134
Fragments arranged by size:
2134 1873 1030 689
BbeI G_GCGC'C
Cuts at: 3160 3408 3160
Size: 248 5478
BbsI GAAGACnn'nnnn_
Cuts at: 231 231
Size: 5726
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 5726
BciVI GTATCCnnnnn_n'
Cuts at: 3624 4385 5726 3624
Size: 761 1341 3624
Fragments arranged by size:
3624 1341 761
BclI T'GATC_A
Cuts at: 3246 3246
Size: 5726
BfrBI ATG'CAT
Cuts at: 2625 2625
Size: 5726
BglI GCCn_nnn'nGGC
Cuts at: 1147 1147
Size: 5726
BglII A'GATC_T
Cuts at: 3241 5278 3241
Size: 2037 3689
BlpI GC'TnA_GC
Cuts at: 2316 2838 2316
Size: 522 5204
Bme1580I G_kGCm'C
Cuts at: 425 1084 2021 3351 3444 4500 425
Size: 659 937 1330 93 1056 1651
Fragments arranged by size:
1651 1330 1056 937 659 93
BmrI ACTGGGnnnn_n'
Cuts at: 1906 2938 3019 3354 5498 1906
Size: 1032 81 335 2144 2134
Fragments arranged by size:
2144 2134 1032 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4093 4093
Size: 5726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 361 565 2077 168
Size: 193 204 1512 3817
Fragments arranged by size:
3817 1512 204 193
Bpu10I CC'TnA_GC
Cuts at: 1490 2108 1490
Size: 618 5108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 959 1054 1516 2902 3781 4273 4571 959
Size: 95 462 1386 879 492 298 2114
Fragments arranged by size:
2114 1386 879 492 462 298 95
BsaI GGTCTCn'nnnn_
Cuts at: 5430 5430
Size: 5726
BsaAI yAC'GTr
Cuts at: 2207 2718 3079 2207
Size: 511 361 4854
Fragments arranged by size:
4854 511 361
BsaBI GATnn'nnATC
Cuts at: 6 3240 5277 6
Size: 3234 2037 455
Fragments arranged by size:
3234 2037 455
BsaHI Gr'CG_yC
Cuts at: 1459 3157 3405 4107 5603 1459
Size: 1698 248 702 1496 1582
Fragments arranged by size:
1698 1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2915 3436 4114 4388 4535 4946 2915
Size: 521 678 274 147 411 3695
Fragments arranged by size:
3695 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1932 1962 2130 2160 1932
Size: 30 168 30 5498
Fragments arranged by size:
5498 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 1430 2150 206
Size: 1224 720 3782
Fragments arranged by size:
3782 1224 720
BseYI C'CCAG_C
Cuts at: 1166 1240 1396 1508 1646 1850 2014 2084 3151
Size: 74 156 112 138 204 164 70 1067
Cuts at: 3151 3909 4486 4982 1166
Size: 758 577 496 1910
Fragments arranged by size:
1910 1067 758 577 496 204 164 156
138 112 74 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2072
Size: 5726
BsiEI CG_ry'CG
Cuts at: 104 3268 3314 4522 104
Size: 3164 46 1208 1308
Fragments arranged by size:
3164 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 494 581 807 2740 3518 3708 4500 494
Size: 87 226 1933 778 190 792 1720
Fragments arranged by size:
1933 1720 792 778 226 190 87
Bsp1286I G_dGCh'C
Cuts at: 178 425 494 581 807 867 1084 2021 2740
Size: 247 69 87 226 60 217 937 719
Cuts at: 2740 3351 3444 3518 3708 3770 4500 178
Size: 611 93 74 190 62 730 1404
Fragments arranged by size:
1404 937 730 719 611 247 226 217
190 93 87 74 69 62 60
BspEI T'CCGG_A
Cuts at: 4946 4946
Size: 5726
BspHI T'CATG_A
Cuts at: 4902 4902
Size: 5726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 249 2776 2990 3292 3673 249
Size: 2527 214 302 381 2302
Fragments arranged by size:
2527 2302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1489 4018 4072 5556 5721 50
Size: 1439 2529 54 1484 165 55
Fragments arranged by size:
2529 1484 1439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 330 3638 330
Size: 3308 2418
BsrFI r'CCGG_y
Cuts at: 1578 3724 3905 4114 1578
Size: 2146 181 209 3190
Fragments arranged by size:
3190 2146 209 181
BsrGI T'GTAC_A
Cuts at: 824 1095 824
Size: 271 5455
BssSI C'ACGA_G
Cuts at: 3997 4355 3997
Size: 358 5368
BstAPI GCAn_nnn'nTGC
Cuts at: 1707 3103 1707
Size: 1396 4330
BstBI TT'CG_AA
Cuts at: 110 4087 110
Size: 3977 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2014 2014
Size: 5726
BstYI r'GATC_y
Cuts at: 1 411 1188 1336 1975 2559 2751 3218 3241
Size: 410 777 148 639 584 192 467 23
Cuts at: 3241 3576 3822 4834 4920 4932 5278 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 777 639 584 467 449 410 346
335 246 192 148 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1325 1325
Size: 5726
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3676 3857 4020 3676
Size: 181 163 5382
Fragments arranged by size:
5382 181 163
BtsI GCAGTG_nn'
Cuts at: 225 984 2969 225
Size: 759 1985 2982
Fragments arranged by size:
2982 1985 759
DraI TTT'AAA
Cuts at: 2725 2725
Size: 5726
DrdI GACnn_nn'nnGTC
Cuts at: 3432 4290 3432
Size: 858 4868
EaeI y'GGCC_r
Cuts at: 519 1766 3311 3485 3876 3903 5248 519
Size: 1247 1545 174 391 27 1345 997
Fragments arranged by size:
1545 1345 1247 997 391 174 27
EagI C'GGCC_G
Cuts at: 3311 3311
Size: 5726
EarI CTCTTCn'nnn_
Cuts at: 228 1294 1531 2474 2534 3749 3959 228
Size: 1066 237 943 60 1215 210 1995
Fragments arranged by size:
1995 1215 1066 943 237 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 298 3934 4244 4390 5536 298
Size: 3636 310 146 1146 488
Fragments arranged by size:
3636 1146 488 310 146
EcoICRI GAG'CTC
Cuts at: 2738 2738
Size: 5726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 361 565 676 2077 2177 2270 2600 3551
Size: 193 204 111 1401 100 93 330 951
Cuts at: 3551 3983 4730 4998 168
Size: 432 747 268 896
Fragments arranged by size:
1401 951 896 747 432 330 268 204
193 111 100 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 5726
EcoO109I rG'GnC_Cy
Cuts at: 651 2064 2865 651
Size: 1413 801 3512
Fragments arranged by size:
3512 1413 801
EcoRI G'AATT_C
Cuts at: 2730 2730
Size: 5726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3745 3745
Size: 5726
FspI TGC'GCA
Cuts at: 3507 3507
Size: 5726
HaeII r_GCGC'y
Cuts at: 549 719 1288 3160 3408 4430 5409 549
Size: 170 569 1872 248 1022 979 866
Fragments arranged by size:
1872 1022 979 866 569 248 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 2218 2250 3568 3598 3600 3630 245
Size: 32 1941 32 1318 30 2 30 2341
Fragments arranged by size:
2341 1941 1318 32 32 30 30 2
HincII GTy'rAC
Cuts at: 360 819 2667 2765 360
Size: 459 1848 98 3321
Fragments arranged by size:
3321 1848 459 98
HindIII A'AGCT_T
Cuts at: 1120 2278 2305 1120
Size: 1158 27 4541
Fragments arranged by size:
4541 1158 27
HpaI GTT'AAC
Cuts at: 2667 2667
Size: 5726
KasI G'GCGC_C
Cuts at: 3156 3404 3156
Size: 248 5478
KpnI G_GTAC'C
Cuts at: 2746 2746
Size: 5726
MfeI C'AATT_G
Cuts at: 1214 1658 1214
Size: 444 5282
MluI A'CGCG_T
Cuts at: 5159 5159
Size: 5726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 986 1953 4397 4581 986
Size: 967 2444 184 2131
Fragments arranged by size:
2444 2131 967 184
MscI TGG'CCA
Cuts at: 521 1768 3487 521
Size: 1247 1719 2760
Fragments arranged by size:
2760 1719 1247
MslI CAynn'nnrTG
Cuts at: 499 3715 3842 499
Size: 3216 127 2383
Fragments arranged by size:
3216 2383 127
MspA1I CmG'CkG
Cuts at: 188 1153 1764 1791 1864 2088 2101 2107 2248
Size: 965 611 27 73 224 13 6 141
Cuts at: 2248 2320 2837 3151 3511 4524 4769 188
Size: 72 517 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 965 611 517 360 314 245
224 141 73 72 27 13 6
NaeI GCC'GGC
Cuts at: 1580 3907 1580
Size: 2327 3399
NarI GG'CG_CC
Cuts at: 3157 3405 3157
Size: 248 5478
NgoMIV G'CCGG_C
Cuts at: 1578 3905 1578
Size: 2327 3399
NsiI A_TGCA'T
Cuts at: 2627 2627
Size: 5726
NspI r_CATG'y
Cuts at: 354 984 2779 3279 4186 354
Size: 630 1795 500 907 1894
Fragments arranged by size:
1894 1795 907 630 500
PasI CC'CwG_GG
Cuts at: 1476 2676 1476
Size: 1200 4526
PciI A'CATG_T
Cuts at: 4182 4182
Size: 5726
PflMI CCAn_nnn'nTGG
Cuts at: 2676 2676
Size: 5726
PmeI GTTT'AAAC
Cuts at: 2725 2725
Size: 5726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4865 4897 4865
Size: 32 5694
PpuMI rG'GwC_Cy
Cuts at: 651 2064 651
Size: 1413 4313
PsiI TTA'TAA
Cuts at: 2186 2186
Size: 5726
PstI C_TGCA'G
Cuts at: 1335 1869 2106 2773 3458 1335
Size: 534 237 667 685 3603
Fragments arranged by size:
3603 685 667 534 237
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5726
PvuII CAG'CTG
Cuts at: 1153 1764 1791 1864 2088 2101 2107 2248 2320
Size: 611 27 73 224 13 6 141 72
Cuts at: 2320 3151 3511 1153
Size: 831 360 3368
Fragments arranged by size:
3368 831 611 360 224 141 73 72
27 13 6
RsrII CG'GwC_CG
Cuts at: 3921 3921
Size: 5726
SacI G_AGCT'C
Cuts at: 2740 2740
Size: 5726
SalI G'TCGA_C
Cuts at: 2763 2763
Size: 5726
SanDI GG'GwC_CC
Cuts at: 2064 2064
Size: 5726
SapI GCTCTTCn'nnn_
Cuts at: 3749 3959 3749
Size: 210 5516
SbfI CC_TGCA'GG
Cuts at: 2773 2773
Size: 5726
ScaI AGT'ACT
Cuts at: 5243 5243
Size: 5726
SexAI A'CCwGG_T
Cuts at: 1040 1040
Size: 5726
SfcI C'TryA_G
Cuts at: 33 1331 1865 2102 2769 3454 4447 4638 33
Size: 1298 534 237 667 685 993 191 1121
Fragments arranged by size:
1298 1121 993 685 667 534 237 191
SfoI GGC'GCC
Cuts at: 3158 3406 3158
Size: 248 5478
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
SmaI CCC'GGG
Cuts at: 2748 2748
Size: 5726
SmlI C'TyrA_G
Cuts at: 974 1069 1531 2881 3796 4288 4550 974
Size: 95 462 1350 915 492 262 2150
Fragments arranged by size:
2150 1350 915 492 462 262 95
SnaBI TAC'GTA
Cuts at: 2718 2718
Size: 5726
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5726
SphI G_CATG'C
Cuts at: 2779 3279 2779
Size: 500 5226
SspI AAT'ATT
Cuts at: 432 432
Size: 5726
StuI AGG'CCT
Cuts at: 1233 1233
Size: 5726
StyI C'CwwG_G
Cuts at: 1683 2352 2705 2860 3214 1683
Size: 669 353 155 354 4195
Fragments arranged by size:
4195 669 354 353 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 698 1410 3309 3442 3901 4110 698
Size: 712 1899 133 459 209 2314
Fragments arranged by size:
2314 1899 712 459 209 133
TatI w'GTAC_w
Cuts at: 824 1095 1632 5241 824
Size: 271 537 3609 1309
Fragments arranged by size:
3609 1309 537 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 321 353 5243 5275 321
Size: 32 4890 32 772
Fragments arranged by size:
4890 772 32 32
Tth111I GACn'n_nGTC
Cuts at: 3523 3523
Size: 5726
XbaI T'CTAG_A
Cuts at: 2757 2757
Size: 5726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3221 3221
Size: 5726
XmaI C'CCGG_G
Cuts at: 2746 2746
Size: 5726
XmnI GAAnn'nnTTC
Cuts at: 136 136
Size: 5726
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI NcoI NdeI NheI NotI NruI PacI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SwaI XhoI ZraI