Restriction map for
pF1KSDA1058
(Circular) (Six-base) MAPSORT of: pF1KSDA1058.seq Check: 9708 from: 1 to: 9343
pF1KSDA1058 9343 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 599 1376 2587 4427 6379 599
Size: 777 1211 1840 1952 3563
Fragments arranged by size:
3563 1952 1840 1211 777
Acc65I G'GTAC_C
Cuts at: 6357 6357
Size: 9343
AclI AA'CG_TT
Cuts at: 9183 9183
Size: 9343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 1666 3013 4157 5160 7166 7598 8347 8615
Size: 111 1347 1144 1003 2006 432 749 268
Cuts at: 8615 1555
Size: 2283
Fragments arranged by size:
2283 2006 1347 1144 1003 749 432 268
111
AfeI AGC'GCT
Cuts at: 5311 5311
Size: 9343
AflII C'TTAA_G
Cuts at: 2164 2680 4488 6169 2164
Size: 516 1808 1681 5338
Fragments arranged by size:
5338 1808 1681 516
AflIII A'CryG_T
Cuts at: 2873 4541 6693 7799 8776 2873
Size: 1668 2152 1106 977 3440
Fragments arranged by size:
3440 2152 1668 1106 977
AgeI A'CCGG_T
Cuts at: 7731 7731
Size: 9343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2566 5046 2566
Size: 2480 6863
AlwNI CAG_nnn'CTG
Cuts at: 318 415 4218 4380 4761 6646 8215 318
Size: 97 3803 162 381 1885 1569 1446
Fragments arranged by size:
3803 1885 1569 1446 381 162 97
ApaI G_GGCC'C
Cuts at: 244 244
Size: 9343
ApaLI G'TGCA_C
Cuts at: 8113 8113
Size: 9343
ApoI r'AATT_y
Cuts at: 765 1993 2128 2248 2558 3322 3981 5603 5638
Size: 1228 135 120 310 764 659 1622 35
Cuts at: 5638 6345 8696 8952 765
Size: 707 2351 256 1156
Fragments arranged by size:
2351 1622 1228 1156 764 707 659 310
256 135 120 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 9343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9343
AvaI C'yCGr_G
Cuts at: 237 2798 4954 5442 6250 6361 237
Size: 2561 2156 488 808 111 3219
Fragments arranged by size:
3219 2561 2156 808 488 111
BamHI G'GATC_C
Cuts at: 751 1223 4991 6366 751
Size: 472 3768 1375 3728
Fragments arranged by size:
3768 3728 1375 472
BanI G'GyrC_C
Cuts at: 3805 6357 6771 7019 7054 3805
Size: 2552 414 248 35 6094
Fragments arranged by size:
6094 2552 414 248 35
BanII G_rGCy'C
Cuts at: 238 244 875 1453 2649 4597 4961 5273 5321
Size: 6 631 578 1196 1948 364 312 48
Cuts at: 5321 6008 6355 7385 238
Size: 687 347 1030 2196
Fragments arranged by size:
2196 1948 1196 1030 687 631 578 364
347 312 48 6
BbeI G_GCGC'C
Cuts at: 3809 6775 7023 3809
Size: 2966 248 6129
Fragments arranged by size:
6129 2966 248
BbsI GAAGACnn'nnnn_
Cuts at: 313 2156 3198 3240 4470 4972 5228 6248 313
Size: 1843 1042 42 1230 502 256 1020 3408
Fragments arranged by size:
3408 1843 1230 1042 1020 502 256 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 417 5305 5339 383
Size: 34 4888 34 4387
Fragments arranged by size:
4888 4387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1505 4841 6322 7239 8002 9343 1505
Size: 3336 1481 917 763 1341 1505
Fragments arranged by size:
3336 1505 1481 1341 917 763
BclI T'GATC_A
Cuts at: 3023 6041 6861 3023
Size: 3018 820 5505
Fragments arranged by size:
5505 3018 820
BfrBI ATG'CAT
Cuts at: 1729 4200 6315 1729
Size: 2471 2115 4757
Fragments arranged by size:
4757 2471 2115
BglI GCCn_nnn'nGGC
Cuts at: 1660 5056 5147 1660
Size: 3396 91 5856
Fragments arranged by size:
5856 3396 91
BglII A'GATC_T
Cuts at: 785 6856 8895 785
Size: 6071 2039 1233
Fragments arranged by size:
6071 2039 1233
BlpI GC'TnA_GC
Cuts at: 1181 1595 1850 2885 3038 4591 6453 1181
Size: 414 255 1035 153 1553 1862 4071
Fragments arranged by size:
4071 1862 1553 1035 414 255 153
Bme1580I G_kGCm'C
Cuts at: 244 2003 6966 7059 8117 244
Size: 1759 4963 93 1058 1470
Fragments arranged by size:
4963 1759 1470 1058 93
BmgBI CAC'GTC
Cuts at: 1418 4970 5226 5733 1418
Size: 3552 256 507 5028
Fragments arranged by size:
5028 3552 507 256
BmrI ACTGGGnnnn_n'
Cuts at: 1852 2817 6553 6634 6969 9115 1852
Size: 965 3736 81 335 2146 2080
Fragments arranged by size:
3736 2146 2080 965 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2169 3902 3904 5226 7708 2169
Size: 1733 2 1322 2482 3804
Fragments arranged by size:
3804 2482 1733 1322 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 2663 3308 3400 3484 4903 6046 946
Size: 1717 645 92 84 1419 1143 4243
Fragments arranged by size:
4243 1717 1419 1143 645 92 84
Bpu10I CC'TnA_GC
Cuts at: 212 4960 6213 212
Size: 4748 1253 3342
Fragments arranged by size:
4748 3342 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1447 1795 2325 2874 2938 3526 4555 5296
Size: 588 348 530 549 64 588 1029 741
Cuts at: 5296 6517 7396 7890 8188 859
Size: 1221 879 494 298 2014
Fragments arranged by size:
2014 1221 1029 879 741 588 588 549
530 494 348 298 64
BsaI GGTCTCn'nnnn_
Cuts at: 2457 3284 9047 2457
Size: 827 5763 2753
Fragments arranged by size:
5763 2753 827
BsaAI yAC'GTr
Cuts at: 1374 2709 5043 6333 6694 1374
Size: 1335 2334 1290 361 4023
Fragments arranged by size:
4023 2334 1335 1290 361
BsaBI GATnn'nnATC
Cuts at: 6 3529 6855 8894 6
Size: 3523 3326 2039 455
Fragments arranged by size:
3523 3326 2039 455
BsaHI Gr'CG_yC
Cuts at: 3754 3806 6772 7020 7724 9220 3754
Size: 52 2966 248 704 1496 3877
Fragments arranged by size:
3877 2966 1496 704 248 52
BsaWI w'CCGG_w
Cuts at: 1051 1349 2517 4676 6530 7051 7731 8005 8152
Size: 298 1168 2159 1854 521 680 274 147
Cuts at: 8152 8563 1051
Size: 411 1831
Fragments arranged by size:
2159 1854 1831 1168 680 521 411 298
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3828 3858 3828
Size: 30 9313
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 4809 5148 5151 5218 6012 607
Size: 4202 339 3 67 794 3938
Fragments arranged by size:
4202 3938 794 339 67 3
BseYI C'CCAG_C
Cuts at: 3801 4218 4616 6324 6766 7524 8103 8599 3801
Size: 417 398 1708 442 758 579 496 4545
Fragments arranged by size:
4545 1708 758 579 496 442 417 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4132 4267 5389 3772
Size: 360 135 1122 7726
Fragments arranged by size:
7726 1122 360 135
BsiEI CG_ry'CG
Cuts at: 104 6029 6883 6929 8139 104
Size: 5925 854 46 1210 1308
Fragments arranged by size:
5925 1308 1210 854 46
BsiHKAI G_wGCw'C
Cuts at: 875 3542 4597 4961 5273 5321 6008 6022 6355
Size: 2667 1055 364 312 48 687 14 333
Cuts at: 6355 7133 7323 8117 875
Size: 778 190 794 2101
Fragments arranged by size:
2667 2101 1055 794 778 687 364 333
312 190 48 14
BsmI GAATG_Cn'
Cuts at: 1656 1694 3561 1656
Size: 38 1867 7438
Fragments arranged by size:
7438 1867 38
BsmBI CGTCTCn'nnnn_
Cuts at: 375 711 375
Size: 336 9007
Bsp1286I G_dGCh'C
Cuts at: 238 244 875 1453 2003 2649 3542 4597 4961
Size: 6 631 578 550 646 893 1055 364
Cuts at: 4961 5273 5321 6008 6022 6355 6966 7059 7133
Size: 312 48 687 14 333 611 93 74
Cuts at: 7133 7323 7385 8117 238
Size: 190 62 732 1464
Fragments arranged by size:
1464 1055 893 732 687 646 631 611
578 550 364 333 312 190 93 74
62 48 14 6
BspEI T'CCGG_A
Cuts at: 2517 4676 8563 2517
Size: 2159 3887 3297
Fragments arranged by size:
3887 3297 2159
BspHI T'CATG_A
Cuts at: 1476 4406 8519 1476
Size: 2930 4113 2300
Fragments arranged by size:
4113 2930 2300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1076 1966 3557 3629 3806 6391 6605 6907 7288
Size: 890 1591 72 177 2585 214 302 381
Cuts at: 7288 1076
Size: 3131
Fragments arranged by size:
3131 2585 1591 890 381 302 214 177
72
BsrBI CCG'CTC
Cuts at: 50 4537 7633 7687 9173 9338 50
Size: 4487 3096 54 1486 165 55
Fragments arranged by size:
4487 3096 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 989 1245 1267 1587 3818 4307 5033 5321 7253
Size: 256 22 320 2231 489 726 288 1932
Cuts at: 7253 989
Size: 3079
Fragments arranged by size:
3079 2231 1932 726 489 320 288 256
22
BsrFI r'CCGG_y
Cuts at: 249 4793 7339 7520 7731 249
Size: 4544 2546 181 211 1861
Fragments arranged by size:
4544 2546 1861 211 181
BsrGI T'GTAC_A
Cuts at: 665 665
Size: 9343
BssSI C'ACGA_G
Cuts at: 7612 7972 7612
Size: 360 8983
BstAPI GCAn_nnn'nTGC
Cuts at: 6718 6718
Size: 9343
BstBI TT'CG_AA
Cuts at: 110 110
Size: 9343
BstXI CCAn_nnnn'nTGG
Cuts at: 640 1700 2494 2571 4258 5970 640
Size: 1060 794 77 1687 1712 4013
Fragments arranged by size:
4013 1712 1687 1060 794 77
BstYI r'GATC_y
Cuts at: 1 314 751 785 956 1223 3883 4991 5694
Size: 313 437 34 171 267 2660 1108 703
Cuts at: 5694 5788 6366 6833 6856 7191 7437 8451 8537
Size: 94 578 467 23 335 246 1014 86
Cuts at: 8537 8549 8895 1
Size: 12 346 449
Fragments arranged by size:
2660 1108 1014 703 578 467 449 437
346 335 313 267 246 171 94 86
34 23 12
BstZ17I GTA'TAC
Cuts at: 2588 2588
Size: 9343
Bsu36I CC'TnA_GG
Cuts at: 2655 5131 5690 2655
Size: 2476 559 6308
Fragments arranged by size:
6308 2476 559
BtgI C'CryG_G
Cuts at: 3986 4389 3986
Size: 403 8940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 761 2710 7291 7472 7635 761
Size: 1949 4581 181 163 2469
Fragments arranged by size:
4581 2469 1949 181 163
BtsI GCAGTG_nn'
Cuts at: 1446 2548 3116 4481 5287 5872 5898 6584 1446
Size: 1102 568 1365 806 585 26 686 4205
Fragments arranged by size:
4205 1365 1102 806 686 585 568 26
DraI TTT'AAA
Cuts at: 4469 6340 4469
Size: 1871 7472
DrdI GACnn_nn'nnGTC
Cuts at: 389 595 705 7047 7907 389
Size: 206 110 6342 860 1825
Fragments arranged by size:
6342 1825 860 206 110
EaeI y'GGCC_r
Cuts at: 371 1550 4260 4382 4865 4931 4985 5972 6026
Size: 1179 2710 122 483 66 54 987 54
Cuts at: 6026 6926 7100 7491 7518 8865 371
Size: 900 174 391 27 1347 849
Fragments arranged by size:
2710 1347 1179 987 900 849 483 391
174 122 66 54 54 27
EagI C'GGCC_G
Cuts at: 6026 6926 6026
Size: 900 8443
EarI CTCTTCn'nnn_
Cuts at: 352 422 1199 1626 2356 3931 5836 5855 7364
Size: 70 777 427 730 1575 1905 19 1509
Cuts at: 7364 7574 352
Size: 210 2121
Fragments arranged by size:
2121 1905 1575 1509 777 730 427 210
70 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 5791 6016 7549 7861 8007 9153 439
Size: 5352 225 1533 312 146 1146 629
Fragments arranged by size:
5352 1533 1146 629 312 225 146
EcoICRI GAG'CTC
Cuts at: 873 4595 4959 5271 5319 6006 6353 873
Size: 3722 364 312 48 687 347 3863
Fragments arranged by size:
3863 3722 687 364 347 312 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1555 1666 2663 3013 3308 3400 3484 4157
Size: 609 111 997 350 295 92 84 673
Cuts at: 4157 4903 5160 6046 7166 7598 8347 8615 946
Size: 746 257 886 1120 432 749 268 1674
Fragments arranged by size:
1674 1120 997 886 749 746 673 609
432 350 295 268 257 111 92 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3790 5130 3790
Size: 1340 8003
EcoO109I rG'GnC_Cy
Cuts at: 241 1288 3795 4493 5462 6480 241
Size: 1047 2507 698 969 1018 3104
Fragments arranged by size:
3104 2507 1047 1018 969 698
EcoRI G'AATT_C
Cuts at: 2128 6345 2128
Size: 4217 5126
EcoRV GAT'ATC
Cuts at: 6145 6145
Size: 9343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 3321 4686 7360 1185
Size: 2136 1365 2674 3168
Fragments arranged by size:
3168 2674 2136 1365
FspI TGC'GCA
Cuts at: 5286 7122 5286
Size: 1836 7507
HaeII r_GCGC'y
Cuts at: 1429 2213 3809 5126 5313 6775 7023 8047 9026
Size: 784 1596 1317 187 1462 248 1024 979
Cuts at: 9026 1429
Size: 1746
Fragments arranged by size:
1746 1596 1462 1317 1024 979 784 248
187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 1159 1191 7183 7213 7215 7245 604
Size: 32 523 32 5992 30 2 30 2702
Fragments arranged by size:
5992 2702 523 32 32 30 30 2
HincII GTy'rAC
Cuts at: 588 600 2790 5685 6380 588
Size: 12 2190 2895 695 3551
Fragments arranged by size:
3551 2895 2190 695 12
HindIII A'AGCT_T
Cuts at: 1162 6197 1162
Size: 5035 4308
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 9343
KasI G'GCGC_C
Cuts at: 3805 6771 7019 3805
Size: 2966 248 6129
Fragments arranged by size:
6129 2966 248
KpnI G_GTAC'C
Cuts at: 6361 6361
Size: 9343
MfeI C'AATT_G
Cuts at: 1886 1886
Size: 9343
MluI A'CGCG_T
Cuts at: 2873 8776 2873
Size: 5903 3440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 5579 8014 8198 536
Size: 5043 2435 184 1681
Fragments arranged by size:
5043 2435 1681 184
MscI TGG'CCA
Cuts at: 1552 4262 4867 4933 4987 5974 7102 1552
Size: 2710 605 66 54 987 1128 3793
Fragments arranged by size:
3793 2710 1128 987 605 66 54
MslI CAynn'nnrTG
Cuts at: 578 694 1016 3229 3535 3706 4256 5393 5722
Size: 116 322 2213 306 171 550 1137 329
Cuts at: 5722 5968 7330 7457 578
Size: 246 1362 127 2464
Fragments arranged by size:
2464 2213 1362 1137 550 329 322 306
246 171 127 116
MspA1I CmG'CkG
Cuts at: 1180 2286 2330 2745 3657 3736 3747 4218 4620
Size: 1106 44 415 912 79 11 471 402
Cuts at: 4620 4713 5595 6015 6324 6452 6766 7126 8141
Size: 93 882 420 309 128 314 360 1015
Cuts at: 8141 8386 1180
Size: 245 2137
Fragments arranged by size:
2137 1106 1015 912 882 471 420 415
402 360 314 309 245 128 93 79
44 11
NaeI GCC'GGC
Cuts at: 251 7522 251
Size: 7271 2072
NarI GG'CG_CC
Cuts at: 3806 6772 7020 3806
Size: 2966 248 6129
Fragments arranged by size:
6129 2966 248
NcoI C'CATG_G
Cuts at: 4389 4389
Size: 9343
NdeI CA'TA_TG
Cuts at: 400 2862 2892 6105 400
Size: 2462 30 3213 3638
Fragments arranged by size:
3638 3213 2462 30
NgoMIV G'CCGG_C
Cuts at: 249 7520 249
Size: 7271 2072
NruI TCG'CGA
Cuts at: 7704 7704
Size: 9343
NsiI A_TGCA'T
Cuts at: 1731 4202 6317 1731
Size: 2471 2115 4757
Fragments arranged by size:
4757 2471 2115
NspI r_CATG'y
Cuts at: 1667 1809 2695 4065 4204 4545 6394 6894 7803
Size: 142 886 1370 139 341 1849 500 909
Cuts at: 7803 1667
Size: 3207
Fragments arranged by size:
3207 1849 1370 909 886 500 341 142
139
PciI A'CATG_T
Cuts at: 4541 7799 4541
Size: 3258 6085
PflMI CCAn_nnn'nTGG
Cuts at: 3462 4164 4380 3462
Size: 702 216 8425
Fragments arranged by size:
8425 702 216
PfoI T'CCnGG_A
Cuts at: 3285 3500 3509 3285
Size: 215 9 9119
Fragments arranged by size:
9119 215 9
PmeI GTTT'AAAC
Cuts at: 6340 6340
Size: 9343
PmlI CAC'GTG
Cuts at: 2709 2709
Size: 9343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1375 1407 5570 5602 8482 8514 1375
Size: 32 4163 32 2880 32 2204
Fragments arranged by size:
4163 2880 2204 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1288 3795 4493 5462 1288
Size: 2507 698 969 5169
Fragments arranged by size:
5169 2507 969 698
PshAI GACnn'nnGTC
Cuts at: 2800 5431 5731 2800
Size: 2631 300 6412
Fragments arranged by size:
6412 2631 300
PsiI TTA'TAA
Cuts at: 773 4505 773
Size: 3732 5611
PspOMI G'GGCC_C
Cuts at: 240 240
Size: 9343
PspXI vC'TCGA_Gb
Cuts at: 6250 6250
Size: 9343
PstI C_TGCA'G
Cuts at: 1963 2223 3451 6053 6388 7073 1963
Size: 260 1228 2602 335 685 4233
Fragments arranged by size:
4233 2602 1228 685 335 260
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9343
PvuII CAG'CTG
Cuts at: 1180 2286 2330 2745 3736 4218 4620 4713 6015
Size: 1106 44 415 991 482 402 93 1302
Cuts at: 6015 6324 6766 7126 1180
Size: 309 442 360 3397
Fragments arranged by size:
3397 1302 1106 991 482 442 415 402
360 309 93 44
RsrII CG'GwC_CG
Cuts at: 7536 7536
Size: 9343
SacI G_AGCT'C
Cuts at: 875 4597 4961 5273 5321 6008 6355 875
Size: 3722 364 312 48 687 347 3863
Fragments arranged by size:
3863 3722 687 364 347 312 48
SalI G'TCGA_C
Cuts at: 598 6378 598
Size: 5780 3563
SapI GCTCTTCn'nnn_
Cuts at: 352 3931 5855 7364 7574 352
Size: 3579 1924 1509 210 2121
Fragments arranged by size:
3579 2121 1924 1509 210
SbfI CC_TGCA'GG
Cuts at: 6388 6388
Size: 9343
ScaI AGT'ACT
Cuts at: 2068 2623 2998 8860 2068
Size: 555 375 5862 2551
Fragments arranged by size:
5862 2551 555 375
SexAI A'CCwGG_T
Cuts at: 2184 2184
Size: 9343
SfcI C'TryA_G
Cuts at: 33 1770 1959 2219 2579 3447 5415 6049 6279
Size: 1737 189 260 360 868 1968 634 230
Cuts at: 6279 6384 7069 8064 8255 33
Size: 105 685 995 191 1121
Fragments arranged by size:
1968 1737 1121 995 868 685 634 360
260 230 191 189 105
SfoI GGC'GCC
Cuts at: 3807 6773 7021 3807
Size: 2966 248 6129
Fragments arranged by size:
6129 2966 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9343
SmaI CCC'GGG
Cuts at: 239 6363 239
Size: 6124 3219
SmlI C'TyrA_G
Cuts at: 874 1462 1810 2164 2340 2680 2853 2953 3541
Size: 588 348 354 176 340 173 100 588
Cuts at: 3541 4488 4570 5275 6169 6250 6496 7411 7905
Size: 947 82 705 894 81 246 915 494
Cuts at: 7905 8167 874
Size: 262 2050
Fragments arranged by size:
2050 947 915 894 705 588 588 494
354 348 340 262 246 176 173 100
82 81
SnaBI TAC'GTA
Cuts at: 6333 6333
Size: 9343
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9343
SphI G_CATG'C
Cuts at: 1667 2695 4204 6394 6894 1667
Size: 1028 1509 2190 500 4116
Fragments arranged by size:
4116 2190 1509 1028 500
SrfI GCCC'GGGC
Cuts at: 239 239
Size: 9343
SspI AAT'ATT
Cuts at: 812 1511 2112 812
Size: 699 601 8043
Fragments arranged by size:
8043 699 601
StuI AGG'CCT
Cuts at: 4612 5209 4612
Size: 597 8746
StyI C'CwwG_G
Cuts at: 624 2099 4389 6475 6829 624
Size: 1475 2290 2086 354 3138
Fragments arranged by size:
3138 2290 2086 1475 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 5443 6924 7057 7516 7727 820
Size: 4623 1481 133 459 211 2436
Fragments arranged by size:
4623 2436 1481 459 211 133
TatI w'GTAC_w
Cuts at: 665 2066 2621 2996 3018 4160 4922 5627 8858
Size: 1401 555 375 22 1142 762 705 3231
Cuts at: 8858 665
Size: 1150
Fragments arranged by size:
3231 1401 1150 1142 762 705 555 375
22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2411 2443 3971 4003 5851 5883 8860 8892 2411
Size: 32 1528 32 1848 32 2977 32 2862
Fragments arranged by size:
2977 2862 1848 1528 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 389 7138 389
Size: 6749 2594
XbaI T'CTAG_A
Cuts at: 1875 6372 1875
Size: 4497 4846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5002 6836 5002
Size: 1834 7509
XhoI C'TCGA_G
Cuts at: 6250 6250
Size: 9343
XmaI C'CCGG_G
Cuts at: 237 6361 237
Size: 6124 3219
XmnI GAAnn'nnTTC
Cuts at: 1387 1512 1387
Size: 125 9218
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI AscI AvrII BaeI
BbvCI BmtI BsiWI BssHII BstEII ClaI CspCI DraIII
FseI FspAI NheI NotI PacI PasI PsrI SacII
SanDI SfiI SgrAI SwaI ZraI