(Circular) (Six-base) MAPSORT of: pF1KSDA1059.seq Check: 5433 from: 1 to: 6803 pF1KSDA1059 6803 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 420 1243 1787 2249 420 Size: 823 544 462 4974 Fragments arranged by size: 4974 823 544 462 AccI GT'mk_AC Cuts at: 202 823 3841 202 Size: 621 3018 3164 Fragments arranged by size: 3164 3018 621 Acc65I G'GTAC_C Cuts at: 3819 3819 Size: 6803 AclI AA'CG_TT Cuts at: 6643 6643 Size: 6803 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 680 2133 2184 2724 3272 3436 4628 5060 5807 Size: 1453 51 540 548 164 1192 432 747 Cuts at: 5807 6075 680 Size: 268 1408 Fragments arranged by size: 1453 1408 1192 747 548 540 432 268 164 51 AflIII A'CryG_T Cuts at: 1367 1496 1522 2768 4155 5259 6236 1367 Size: 129 26 1246 1387 1104 977 1934 Fragments arranged by size: 1934 1387 1246 1104 977 129 26 AgeI A'CCGG_T Cuts at: 5191 5191 Size: 6803 AhdI GACnn_n'nnGTC Cuts at: 248 248 Size: 6803 AlwNI CAG_nnn'CTG Cuts at: 490 1023 4108 5675 490 Size: 533 3085 1567 1618 Fragments arranged by size: 3085 1618 1567 533 ApaLI G'TGCA_C Cuts at: 727 1189 1741 5573 727 Size: 462 552 3832 1957 Fragments arranged by size: 3832 1957 552 462 ApoI r'AATT_y Cuts at: 3396 3807 6156 6412 3396 Size: 411 2349 256 3787 Fragments arranged by size: 3787 2349 411 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6803 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6803 AvaI C'yCGr_G Cuts at: 617 2588 3823 617 Size: 1971 1235 3597 Fragments arranged by size: 3597 1971 1235 BamHI G'GATC_C Cuts at: 1241 3828 1241 Size: 2587 4216 BanI G'GyrC_C Cuts at: 168 220 371 633 835 1697 1978 2237 3819 Size: 52 151 262 202 862 281 259 1582 Cuts at: 3819 4233 4481 4516 168 Size: 414 248 35 2455 Fragments arranged by size: 2455 1582 862 414 281 262 259 248 202 151 52 35 BanII G_rGCy'C Cuts at: 191 1877 2100 2310 3222 3817 4847 191 Size: 1686 223 210 912 595 1030 2147 Fragments arranged by size: 2147 1686 1030 912 595 223 210 BbeI G_GCGC'C Cuts at: 172 224 375 637 1701 4237 4485 172 Size: 52 151 262 1064 2536 248 2490 Fragments arranged by size: 2536 2490 1064 262 248 151 52 BbsI GAAGACnn'nnnn_ Cuts at: 668 880 1110 1669 668 Size: 212 230 559 5802 Fragments arranged by size: 5802 559 230 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 669 703 669 Size: 34 6769 BciVI GTATCCnnnnn_n' Cuts at: 4701 5462 6803 4701 Size: 761 1341 4701 Fragments arranged by size: 4701 1341 761 BclI T'GATC_A Cuts at: 962 4323 962 Size: 3361 3442 BfrBI ATG'CAT Cuts at: 1122 1122 Size: 6803 BglI GCCn_nnn'nGGC Cuts at: 155 1987 2016 155 Size: 1832 29 4942 Fragments arranged by size: 4942 1832 29 BglII A'GATC_T Cuts at: 1934 4318 6355 1934 Size: 2384 2037 2382 Fragments arranged by size: 2384 2382 2037 BlpI GC'TnA_GC Cuts at: 3498 3915 3498 Size: 417 6386 Bme1580I G_kGCm'C Cuts at: 731 1193 1745 1983 2319 2575 2642 3491 4428 Size: 462 552 238 336 256 67 849 937 Cuts at: 4428 4521 5577 731 Size: 93 1056 1957 Fragments arranged by size: 1957 1056 937 849 552 462 336 256 238 93 67 BmgBI CAC'GTC Cuts at: 206 206 Size: 6803 BmrI ACTGGGnnnn_n' Cuts at: 1059 1732 1980 2507 2936 2959 4015 4096 4431 Size: 673 248 527 429 23 1056 81 335 Cuts at: 4431 6575 1059 Size: 2144 1287 Fragments arranged by size: 2144 1287 1056 673 527 429 335 248 81 23 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2064 5170 2064 Size: 3106 3697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 3462 3462 Size: 6803 Bpu10I CC'TnA_GC Cuts at: 608 2539 608 Size: 1931 4872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3581 3979 4858 5350 5648 3581 Size: 398 879 492 298 4736 Fragments arranged by size: 4736 879 492 398 298 BsaI GGTCTCn'nnnn_ Cuts at: 2113 2321 2352 3283 6507 2113 Size: 208 31 931 3224 2409 Fragments arranged by size: 3224 2409 931 208 31 BsaAI yAC'GTr Cuts at: 1368 1499 2769 3795 4156 1368 Size: 131 1270 1026 361 4015 Fragments arranged by size: 4015 1270 1026 361 131 BsaBI GATnn'nnATC Cuts at: 6 3586 4317 6354 6 Size: 3580 731 2037 455 Fragments arranged by size: 3580 2037 731 455 BsaHI Gr'CG_yC Cuts at: 169 221 258 343 372 557 634 1698 4234 Size: 52 37 85 29 185 77 1064 2536 Cuts at: 4234 4482 5184 6680 169 Size: 248 702 1496 292 Fragments arranged by size: 2536 1496 1064 702 292 248 185 85 77 52 37 29 BsaWI w'CCGG_w Cuts at: 179 1713 3992 4513 5191 5465 5612 6023 179 Size: 1534 2279 521 678 274 147 411 959 Fragments arranged by size: 2279 1534 959 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 328 358 2404 2434 3447 3477 328 Size: 30 2046 30 1013 30 3654 Fragments arranged by size: 3654 2046 1013 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 349 525 528 637 1896 2683 3345 3706 349 Size: 176 3 109 1259 787 662 361 3446 Fragments arranged by size: 3446 1259 787 662 361 176 109 3 BseYI C'CCAG_C Cuts at: 366 398 483 643 676 2427 3073 3131 3484 Size: 32 85 160 33 1751 646 58 353 Cuts at: 3484 4228 4986 5563 6059 366 Size: 744 758 577 496 1110 Fragments arranged by size: 1751 1110 758 744 646 577 496 353 160 85 58 33 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1759 2511 2948 3765 1759 Size: 752 437 817 4797 Fragments arranged by size: 4797 817 752 437 BsiEI CG_ry'CG Cuts at: 104 578 2612 4345 4391 5599 104 Size: 474 2034 1733 46 1208 1308 Fragments arranged by size: 2034 1733 1308 1208 474 46 BsiHKAI G_wGCw'C Cuts at: 731 1193 1745 1877 2310 3222 3817 4595 4785 Size: 462 552 132 433 912 595 778 190 Cuts at: 4785 5577 731 Size: 792 1957 Fragments arranged by size: 1957 912 792 778 595 552 462 433 190 132 BsmI GAATG_Cn' Cuts at: 3084 3084 Size: 6803 Bsp1286I G_dGCh'C Cuts at: 191 731 1193 1745 1877 1983 2100 2310 2319 Size: 540 462 552 132 106 117 210 9 Cuts at: 2319 2575 2642 3222 3491 3817 4428 4521 4595 Size: 256 67 580 269 326 611 93 74 Cuts at: 4595 4785 4847 5577 191 Size: 190 62 730 1417 Fragments arranged by size: 1417 730 611 580 552 540 462 326 269 256 210 190 132 117 106 93 74 67 62 9 BspEI T'CCGG_A Cuts at: 179 1713 6023 179 Size: 1534 4310 959 Fragments arranged by size: 4310 1534 959 BspHI T'CATG_A Cuts at: 2129 3114 5979 2129 Size: 985 2865 2953 Fragments arranged by size: 2953 2865 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 420 1243 1603 1787 2249 3853 4067 4369 4750 Size: 823 360 184 462 1604 214 302 381 Cuts at: 4750 420 Size: 2473 Fragments arranged by size: 2473 1604 823 462 381 360 302 214 184 BsrBI CCG'CTC Cuts at: 50 465 1286 2159 5095 5149 6633 6798 50 Size: 415 821 873 2936 54 1484 165 55 Fragments arranged by size: 2936 1484 873 821 415 165 55 54 BsrDI GCAATG_nn' Cuts at: 2208 3210 3260 3348 3734 4715 2208 Size: 1002 50 88 386 981 4296 Fragments arranged by size: 4296 1002 981 386 88 50 BsrFI r'CCGG_y Cuts at: 217 285 453 2204 4801 4982 5191 217 Size: 68 168 1751 2597 181 209 1829 Fragments arranged by size: 2597 1829 1751 209 181 168 68 BssHII G'CGCG_C Cuts at: 136 136 Size: 6803 BssSI C'ACGA_G Cuts at: 1984 5074 5432 1984 Size: 3090 358 3355 Fragments arranged by size: 3355 3090 358 BstAPI GCAn_nnn'nTGC Cuts at: 1119 1749 4180 1119 Size: 630 2431 3742 Fragments arranged by size: 3742 2431 630 BstBI TT'CG_AA Cuts at: 110 5164 110 Size: 5054 1749 BstXI CCAn_nnnn'nTGG Cuts at: 2435 2949 2435 Size: 514 6289 BstYI r'GATC_y Cuts at: 1 1241 1934 2786 3254 3770 3828 4295 4318 Size: 1240 693 852 468 516 58 467 23 Cuts at: 4318 4653 4899 5911 5997 6009 6355 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1240 1012 852 693 516 468 467 449 346 335 246 86 58 23 12 Bsu36I CC'TnA_GG Cuts at: 1806 3689 1806 Size: 1883 4920 BtgI C'CryG_G Cuts at: 125 307 637 722 1091 1208 1547 125 Size: 182 330 85 369 117 339 5381 Fragments arranged by size: 5381 369 339 330 182 117 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4753 4934 5097 4753 Size: 181 163 6459 Fragments arranged by size: 6459 181 163 BtsI GCAGTG_nn' Cuts at: 320 566 1743 4046 320 Size: 246 1177 2303 3077 Fragments arranged by size: 3077 2303 1177 246 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 272 307 272 Size: 35 6768 DraI TTT'AAA Cuts at: 3564 3802 3564 Size: 238 6565 DraIII CAC_nnn'GTG Cuts at: 3673 3673 Size: 6803 DrdI GACnn_nn'nnGTC Cuts at: 2836 3721 4509 5367 2836 Size: 885 788 858 4272 Fragments arranged by size: 4272 885 858 788 EaeI y'GGCC_r Cuts at: 215 280 317 328 575 1160 1544 1988 2142 Size: 65 37 11 247 585 384 444 154 Cuts at: 2142 4388 4562 4953 4980 6325 215 Size: 2246 174 391 27 1345 693 Fragments arranged by size: 2246 1345 693 585 444 391 384 247 174 154 65 37 27 11 EagI C'GGCC_G Cuts at: 575 4388 575 Size: 3813 2990 EarI CTCTTCn'nnn_ Cuts at: 1347 1374 1940 2848 4826 5036 1347 Size: 27 566 908 1978 210 3114 Fragments arranged by size: 3114 1978 908 566 210 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1195 2523 5011 5321 5467 6613 1195 Size: 1328 2488 310 146 1146 1385 Fragments arranged by size: 2488 1385 1328 1146 310 146 EcoICRI GAG'CTC Cuts at: 1875 2308 3220 3815 1875 Size: 433 912 595 4863 Fragments arranged by size: 4863 912 595 433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 680 2133 2184 2724 3272 3436 3462 4628 5060 Size: 1453 51 540 548 164 26 1166 432 Cuts at: 5060 5807 6075 680 Size: 747 268 1408 Fragments arranged by size: 1453 1408 1166 747 548 540 432 268 164 51 26 EcoNI CCTnn'n_nnAGG Cuts at: 607 2665 3088 607 Size: 2058 423 4322 Fragments arranged by size: 4322 2058 423 EcoO109I rG'GnC_Cy Cuts at: 210 2592 2954 3351 3942 210 Size: 2382 362 397 591 3071 Fragments arranged by size: 3071 2382 591 397 362 EcoRI G'AATT_C Cuts at: 3807 3807 Size: 6803 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1292 4822 1292 Size: 3530 3273 FspI TGC'GCA Cuts at: 4584 4584 Size: 6803 HaeII r_GCGC'y Cuts at: 150 172 224 375 637 1701 4237 4485 5507 Size: 22 52 151 262 1064 2536 248 1022 Cuts at: 5507 6486 150 Size: 979 467 Fragments arranged by size: 2536 1064 1022 979 467 262 248 151 52 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 234 266 328 360 501 533 2176 2208 2330 Size: 32 62 32 141 32 1643 32 122 Cuts at: 2330 2362 3447 3479 4645 4675 4677 4707 234 Size: 32 1085 32 1166 30 2 30 2330 Fragments arranged by size: 2330 1643 1166 1085 141 122 62 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 203 824 2091 2919 2947 3842 203 Size: 621 1267 828 28 895 3164 Fragments arranged by size: 3164 1267 895 828 621 28 HindIII A'AGCT_T Cuts at: 496 496 Size: 6803 KasI G'GCGC_C Cuts at: 168 220 371 633 1697 4233 4481 168 Size: 52 151 262 1064 2536 248 2490 Fragments arranged by size: 2536 2490 1064 262 248 151 52 KpnI G_GTAC'C Cuts at: 3823 3823 Size: 6803 MfeI C'AATT_G Cuts at: 2170 2370 2170 Size: 200 6603 MluI A'CGCG_T Cuts at: 1522 6236 1522 Size: 4714 2089 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 928 2543 3627 5474 5658 928 Size: 1615 1084 1847 184 2073 Fragments arranged by size: 2073 1847 1615 1084 184 MscI TGG'CCA Cuts at: 319 1546 1990 2144 4564 319 Size: 1227 444 154 2420 2558 Fragments arranged by size: 2558 2420 1227 444 154 MslI CAynn'nnrTG Cuts at: 1198 2662 3265 3508 4792 4919 1198 Size: 1464 603 243 1284 127 3082 Fragments arranged by size: 3082 1464 1284 603 243 127 MspA1I CmG'CkG Cuts at: 309 639 2836 3453 3914 4228 4588 5601 5846 Size: 330 2197 617 461 314 360 1013 245 Cuts at: 5846 309 Size: 1266 Fragments arranged by size: 2197 1266 1013 617 461 360 330 314 245 NaeI GCC'GGC Cuts at: 219 2206 4984 219 Size: 1987 2778 2038 Fragments arranged by size: 2778 2038 1987 NarI GG'CG_CC Cuts at: 169 221 372 634 1698 4234 4482 169 Size: 52 151 262 1064 2536 248 2490 Fragments arranged by size: 2536 2490 1064 262 248 151 52 NcoI C'CATG_G Cuts at: 125 722 1091 1547 125 Size: 597 369 456 5381 Fragments arranged by size: 5381 597 456 369 NgoMIV G'CCGG_C Cuts at: 217 2204 4982 217 Size: 1987 2778 2038 Fragments arranged by size: 2778 2038 1987 NsiI A_TGCA'T Cuts at: 1124 1124 Size: 6803 NspI r_CATG'y Cuts at: 1434 2854 2875 3856 4356 5263 1434 Size: 1420 21 981 500 907 2974 Fragments arranged by size: 2974 1420 981 907 500 21 PasI CC'CwG_GG Cuts at: 2319 2319 Size: 6803 PciI A'CATG_T Cuts at: 5259 5259 Size: 6803 PflMI CCAn_nnn'nTGG Cuts at: 326 723 3399 326 Size: 397 2676 3730 Fragments arranged by size: 3730 2676 397 PfoI T'CCnGG_A Cuts at: 252 1244 1313 3089 252 Size: 992 69 1776 3966 Fragments arranged by size: 3966 1776 992 69 PmeI GTTT'AAAC Cuts at: 3802 3802 Size: 6803 PmlI CAC'GTG Cuts at: 2769 2769 Size: 6803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5942 5974 5942 Size: 32 6771 PpuMI rG'GwC_Cy Cuts at: 210 2954 3351 210 Size: 2744 397 3662 Fragments arranged by size: 3662 2744 397 PshAI GACnn'nnGTC Cuts at: 208 208 Size: 6803 PstI C_TGCA'G Cuts at: 1240 2470 3850 4535 1240 Size: 1230 1380 685 3508 Fragments arranged by size: 3508 1380 1230 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6803 PvuII CAG'CTG Cuts at: 2836 3453 4228 4588 2836 Size: 617 775 360 5051 Fragments arranged by size: 5051 775 617 360 RsrII CG'GwC_CG Cuts at: 4998 4998 Size: 6803 SacI G_AGCT'C Cuts at: 1877 2310 3222 3817 1877 Size: 433 912 595 4863 Fragments arranged by size: 4863 912 595 433 SacII CC_GC'GG Cuts at: 310 640 310 Size: 330 6473 SalI G'TCGA_C Cuts at: 201 822 3840 201 Size: 621 3018 3164 Fragments arranged by size: 3164 3018 621 SanDI GG'GwC_CC Cuts at: 2954 2954 Size: 6803 SapI GCTCTTCn'nnn_ Cuts at: 1347 4826 5036 1347 Size: 3479 210 3114 Fragments arranged by size: 3479 3114 210 SbfI CC_TGCA'GG Cuts at: 1240 3850 1240 Size: 2610 4193 ScaI AGT'ACT Cuts at: 1414 6320 1414 Size: 4906 1897 SexAI A'CCwGG_T Cuts at: 1184 1655 1184 Size: 471 6332 SfcI C'TryA_G Cuts at: 33 1062 1083 1236 1853 2466 3846 4531 5524 Size: 1029 21 153 617 613 1380 685 993 Cuts at: 5524 5715 33 Size: 191 1121 Fragments arranged by size: 1380 1121 1029 993 685 617 613 191 153 21 SfoI GGC'GCC Cuts at: 170 222 373 635 1699 4235 4483 170 Size: 52 151 262 1064 2536 248 2490 Fragments arranged by size: 2536 2490 1064 262 248 151 52 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6803 SgrAI Cr'CCGG_yG Cuts at: 285 285 Size: 6803 SmaI CCC'GGG Cuts at: 619 3825 619 Size: 3206 3597 SmlI C'TyrA_G Cuts at: 3596 3958 4873 5365 5627 3596 Size: 362 915 492 262 4772 Fragments arranged by size: 4772 915 492 362 262 SnaBI TAC'GTA Cuts at: 3795 3795 Size: 6803 SpeI A'CTAG_T Cuts at: 67 1345 3784 67 Size: 1278 2439 3086 Fragments arranged by size: 3086 2439 1278 SphI G_CATG'C Cuts at: 3856 4356 3856 Size: 500 6303 SspI AAT'ATT Cuts at: 2285 3121 2285 Size: 836 5967 StuI AGG'CCT Cuts at: 1387 1387 Size: 6803 StyI C'CwwG_G Cuts at: 125 722 1091 1547 2216 2472 2558 2820 3577 Size: 597 369 456 669 256 86 262 757 Cuts at: 3577 3937 4291 125 Size: 360 354 2637 Fragments arranged by size: 2637 757 669 597 456 369 360 354 262 256 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 556 1041 1152 2016 2626 3327 4386 4519 4978 Size: 485 111 864 610 701 1059 133 459 Cuts at: 4978 5187 556 Size: 209 2172 Fragments arranged by size: 2172 1059 864 701 610 485 459 209 133 111 TatI w'GTAC_w Cuts at: 1412 1444 1949 2549 6318 1412 Size: 32 505 600 3769 1897 Fragments arranged by size: 3769 1897 600 505 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1724 1756 1832 1864 2061 2093 2368 2400 2404 Size: 32 76 32 197 32 275 32 4 Cuts at: 2404 2436 6320 6352 1724 Size: 32 3884 32 2175 Fragments arranged by size: 3884 2175 275 197 76 32 32 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 4600 4600 Size: 6803 XbaI T'CTAG_A Cuts at: 3834 3834 Size: 6803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2704 3070 4298 2572 Size: 132 366 1228 5077 Fragments arranged by size: 5077 1228 366 132 XmaI C'CCGG_G Cuts at: 617 3823 617 Size: 3206 3597 XmnI GAAnn'nnTTC Cuts at: 2284 2284 Size: 6803 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BmtI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI NdeI NheI NotI NruI PacI PsiI PspOMI PspXI PsrI SfiI SrfI SwaI XhoI ZraI