Restriction map for
pF1KSDA1067
(Circular) (Six-base) MAPSORT of: pF1KSDA1067.seq Check: 9479 from: 1 to: 5191
pF1KSDA1067 5191 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 704 2127 704
Size: 1423 3768
AccI GT'mk_AC
Cuts at: 779 2227 779
Size: 1448 3743
Acc65I G'GTAC_C
Cuts at: 2205 2205
Size: 5191
AclI AA'CG_TT
Cuts at: 5031 5031
Size: 5191
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 847 3014 3446 4195 4463 199
Size: 648 2167 432 749 268 927
Fragments arranged by size:
2167 927 749 648 432 268
AfeI AGC'GCT
Cuts at: 1397 1789 1397
Size: 392 4799
AflIII A'CryG_T
Cuts at: 2541 3647 4624 2541
Size: 1106 977 3108
Fragments arranged by size:
3108 1106 977
AgeI A'CCGG_T
Cuts at: 3579 3579
Size: 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1618 1618
Size: 5191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 5159
AlwNI CAG_nnn'CTG
Cuts at: 342 1116 2494 4063 342
Size: 774 1378 1569 1470
Fragments arranged by size:
1569 1470 1378 774
ApaI G_GGCC'C
Cuts at: 439 439
Size: 5191
ApaLI G'TGCA_C
Cuts at: 313 3961 313
Size: 3648 1543
ApoI r'AATT_y
Cuts at: 583 2193 4544 4800 583
Size: 1610 2351 256 974
Fragments arranged by size:
2351 1610 974 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5191
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
AvaI C'yCGr_G
Cuts at: 700 1132 2209 700
Size: 432 1077 3682
Fragments arranged by size:
3682 1077 432
BamHI G'GATC_C
Cuts at: 2214 2214
Size: 5191
BanI G'GyrC_C
Cuts at: 1387 1462 1534 2205 2619 2867 2902 1387
Size: 75 72 671 414 248 35 3676
Fragments arranged by size:
3676 671 414 248 75 72 35
BanII G_rGCy'C
Cuts at: 439 1006 1478 2203 3233 439
Size: 567 472 725 1030 2397
Fragments arranged by size:
2397 1030 725 567 472
BbeI G_GCGC'C
Cuts at: 1538 2623 2871 1538
Size: 1085 248 3858
Fragments arranged by size:
3858 1085 248
BbsI GAAGACnn'nnnn_
Cuts at: 367 1156 1260 1783 2044 367
Size: 789 104 523 261 3514
Fragments arranged by size:
3514 789 523 261 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2155 2189 2155
Size: 34 5157
BciVI GTATCCnnnnn_n'
Cuts at: 3087 3850 5191 3087
Size: 763 1341 3087
Fragments arranged by size:
3087 1341 763
BclI T'GATC_A
Cuts at: 644 2709 644
Size: 2065 3126
BglI GCCn_nnn'nGGC
Cuts at: 1228 1543 1228
Size: 315 4876
BglII A'GATC_T
Cuts at: 2704 4743 2704
Size: 2039 3152
BlpI GC'TnA_GC
Cuts at: 1606 1654 1784 2301 1606
Size: 48 130 517 4496
Fragments arranged by size:
4496 517 130 48
Bme1580I G_kGCm'C
Cuts at: 317 439 1330 2093 2814 2907 3965 317
Size: 122 891 763 721 93 1058 1543
Fragments arranged by size:
1543 1058 891 763 721 122 93
BmgBI CAC'GTC
Cuts at: 681 1047 681
Size: 366 4825
BmrI ACTGGGnnnn_n'
Cuts at: 2401 2482 2817 4963 2401
Size: 81 335 2146 2629
Fragments arranged by size:
2629 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1578 1918 3556 1578
Size: 340 1638 3213
Fragments arranged by size:
3213 1638 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 313 595 685 697 709 780 1306 1420
Size: 72 282 90 12 12 71 526 114
Cuts at: 1420 1756 241
Size: 336 3676
Fragments arranged by size:
3676 526 336 282 114 90 72 71
12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1711 2365 3244 3738 4036 1273
Size: 438 654 879 494 298 2428
Fragments arranged by size:
2428 879 654 494 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 1045 1372 1549 2047 4895 1045
Size: 327 177 498 2848 1341
Fragments arranged by size:
2848 1341 498 327 177
BsaAI yAC'GTr
Cuts at: 2181 2542 2181
Size: 361 4830
BsaBI GATnn'nnATC
Cuts at: 6 2703 4742 6
Size: 2697 2039 455
Fragments arranged by size:
2697 2039 455
BsaHI Gr'CG_yC
Cuts at: 1535 2620 2868 3572 5068 1535
Size: 1085 248 704 1496 1658
Fragments arranged by size:
1658 1496 1085 704 248
BsaWI w'CCGG_w
Cuts at: 2378 2899 3579 3853 4000 4411 2378
Size: 521 680 274 147 411 3158
Fragments arranged by size:
3158 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 1234 1264 184
Size: 30 1020 30 4111
Fragments arranged by size:
4111 1020 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 202
Size: 3 5188
BseYI C'CCAG_C
Cuts at: 974 1629 1680 2614 3372 3951 4447 974
Size: 655 51 934 758 579 496 1718
Fragments arranged by size:
1718 934 758 655 579 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 1687
Size: 5191
BsiEI CG_ry'CG
Cuts at: 104 2157 2731 2777 3987 104
Size: 2053 574 46 1210 1308
Fragments arranged by size:
2053 1308 1210 574 46
BsiHKAI G_wGCw'C
Cuts at: 317 632 695 713 1139 1262 1322 1478 1805
Size: 315 63 18 426 123 60 156 327
Cuts at: 1805 2203 2981 3171 3965 317
Size: 398 778 190 794 1543
Fragments arranged by size:
1543 794 778 426 398 327 315 190
156 123 63 60 18
BsmBI CGTCTCn'nnnn_
Cuts at: 624 1519 624
Size: 895 4296
Bsp1286I G_dGCh'C
Cuts at: 317 439 632 695 713 1006 1139 1262 1322
Size: 122 193 63 18 293 133 123 60
Cuts at: 1322 1330 1478 1805 2093 2203 2814 2907 2981
Size: 8 148 327 288 110 611 93 74
Cuts at: 2981 3171 3233 3965 317
Size: 190 62 732 1543
Fragments arranged by size:
1543 732 611 327 293 288 193 190
148 133 123 122 110 93 74 63
62 60 18 8
BspEI T'CCGG_A
Cuts at: 4411 4411
Size: 5191
BspHI T'CATG_A
Cuts at: 770 4367 770
Size: 3597 1594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 704 1646 2127 2239 2453 2755 3136 704
Size: 942 481 112 214 302 381 2759
Fragments arranged by size:
2759 942 481 381 302 214 112
BsrBI CCG'CTC
Cuts at: 50 151 812 1917 3481 3535 5021 5186 50
Size: 101 661 1105 1564 54 1486 165 55
Fragments arranged by size:
1564 1486 1105 661 165 101 55 54
BsrDI GCAATG_nn'
Cuts at: 1491 3101 1491
Size: 1610 3581
BsrFI r'CCGG_y
Cuts at: 3187 3368 3579 3187
Size: 181 211 4799
Fragments arranged by size:
4799 211 181
BssSI C'ACGA_G
Cuts at: 1471 3460 3820 1471
Size: 1989 360 2842
Fragments arranged by size:
2842 1989 360
BstAPI GCAn_nnn'nTGC
Cuts at: 342 2566 342
Size: 2224 2967
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5191
BstXI CCAn_nnnn'nTGG
Cuts at: 804 1398 804
Size: 594 4597
BstYI r'GATC_y
Cuts at: 1 639 1817 2214 2681 2704 3039 3285 4299
Size: 638 1178 397 467 23 335 246 1014
Cuts at: 4299 4385 4397 4743 1
Size: 86 12 346 449
Fragments arranged by size:
1178 1014 638 467 449 397 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 1253 1373 1253
Size: 120 5071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1845 3139 3320 3483 1845
Size: 1294 181 163 3553
Fragments arranged by size:
3553 1294 181 163
BtsI GCAGTG_nn'
Cuts at: 497 1068 1772 2432 497
Size: 571 704 660 3256
Fragments arranged by size:
3256 704 660 571
ClaI AT'CG_AT
Cuts at: 2153 2153
Size: 5191
DraI TTT'AAA
Cuts at: 2188 2188
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 2895 3755 2895
Size: 860 4331
EaeI y'GGCC_r
Cuts at: 404 473 753 928 1118 1568 2774 2948 3339
Size: 69 280 175 190 450 1206 174 391
Cuts at: 3339 3366 4713 404
Size: 27 1347 882
Fragments arranged by size:
1347 1206 882 450 391 280 190 175
174 69 27
EagI C'GGCC_G
Cuts at: 2774 2774
Size: 5191
EarI CTCTTCn'nnn_
Cuts at: 220 449 1501 1569 3212 3422 220
Size: 229 1052 68 1643 210 1989
Fragments arranged by size:
1989 1643 1052 229 210 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3397 3709 3855 5001 3397
Size: 312 146 1146 3587
Fragments arranged by size:
3587 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1476 2201 1476
Size: 725 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 241 313 595 685 697 709 780 847
Size: 42 72 282 90 12 12 71 67
Cuts at: 847 1306 1420 1756 3014 3446 4195 4463 199
Size: 459 114 336 1258 432 749 268 927
Fragments arranged by size:
1258 927 749 459 432 336 282 268
114 90 72 71 67 42 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1022 1168 1022
Size: 146 5045
EcoO109I rG'GnC_Cy
Cuts at: 435 436 570 1677 2063 2328 435
Size: 1 134 1107 386 265 3298
Fragments arranged by size:
3298 1107 386 265 134 1
EcoRI G'AATT_C
Cuts at: 2193 2193
Size: 5191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1170 3208 1170
Size: 2038 3153
FspI TGC'GCA
Cuts at: 2970 2970
Size: 5191
HaeII r_GCGC'y
Cuts at: 1399 1538 1694 1791 2623 2871 3895 4874 1399
Size: 139 156 97 832 248 1024 979 1716
Fragments arranged by size:
1716 1024 979 832 248 156 139 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 315 347 3031 3061 3063 3093 184
Size: 32 99 32 2684 30 2 30 2282
Fragments arranged by size:
2684 2282 99 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2228 2228
Size: 5191
KasI G'GCGC_C
Cuts at: 1534 2619 2867 1534
Size: 1085 248 3858
Fragments arranged by size:
3858 1085 248
KpnI G_GTAC'C
Cuts at: 2209 2209
Size: 5191
MluI A'CGCG_T
Cuts at: 4624 4624
Size: 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4046 3862
Size: 184 5007
MscI TGG'CCA
Cuts at: 406 475 2950 406
Size: 69 2475 2647
Fragments arranged by size:
2647 2475 69
MslI CAynn'nnrTG
Cuts at: 310 1372 3178 3305 310
Size: 1062 1806 127 2196
Fragments arranged by size:
2196 1806 1062 127
MspA1I CmG'CkG
Cuts at: 339 737 804 1113 1758 1838 2300 2614 2974
Size: 398 67 309 645 80 462 314 360
Cuts at: 2974 3989 4234 339
Size: 1015 245 1296
Fragments arranged by size:
1296 1015 645 462 398 360 314 309
245 80 67
NaeI GCC'GGC
Cuts at: 3370 3370
Size: 5191
NarI GG'CG_CC
Cuts at: 1535 2620 2868 1535
Size: 1085 248 3858
Fragments arranged by size:
3858 1085 248
NcoI C'CATG_G
Cuts at: 1373 1373
Size: 5191
NgoMIV G'CCGG_C
Cuts at: 3368 3368
Size: 5191
NruI TCG'CGA
Cuts at: 3552 3552
Size: 5191
NspI r_CATG'y
Cuts at: 1041 1452 2242 2742 3651 1041
Size: 411 790 500 909 2581
Fragments arranged by size:
2581 909 790 500 411
PciI A'CATG_T
Cuts at: 3647 3647
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 1116 1761 1116
Size: 645 4546
PfoI T'CCnGG_A
Cuts at: 503 713 1520 1904 503
Size: 210 807 384 3790
Fragments arranged by size:
3790 807 384 210
PmeI GTTT'AAAC
Cuts at: 2188 2188
Size: 5191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4330 4362 4330
Size: 32 5159
PpuMI rG'GwC_Cy
Cuts at: 1677 1677
Size: 5191
PshAI GACnn'nnGTC
Cuts at: 2044 2044
Size: 5191
PspOMI G'GGCC_C
Cuts at: 435 435
Size: 5191
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 766 798 766
Size: 32 5159
PstI C_TGCA'G
Cuts at: 338 347 1505 1643 2236 2921 338
Size: 9 1158 138 593 685 2608
Fragments arranged by size:
2608 1158 685 593 138 9
PvuI CG_AT'CG
Cuts at: 104 2157 104
Size: 2053 3138
PvuII CAG'CTG
Cuts at: 804 1113 1758 1838 2614 2974 804
Size: 309 645 80 776 360 3021
Fragments arranged by size:
3021 776 645 360 309 80
RsrII CG'GwC_CG
Cuts at: 3384 3384
Size: 5191
SacI G_AGCT'C
Cuts at: 1478 2203 1478
Size: 725 4466
SalI G'TCGA_C
Cuts at: 2226 2226
Size: 5191
SapI GCTCTTCn'nnn_
Cuts at: 220 1569 3212 3422 220
Size: 1349 1643 210 1989
Fragments arranged by size:
1989 1643 1349 210
SbfI CC_TGCA'GG
Cuts at: 2236 2236
Size: 5191
ScaI AGT'ACT
Cuts at: 4708 4708
Size: 5191
SexAI A'CCwGG_T
Cuts at: 1313 1313
Size: 5191
SfcI C'TryA_G
Cuts at: 33 334 343 1501 1578 1639 2071 2232 2917
Size: 301 9 1158 77 61 432 161 685
Cuts at: 2917 3912 4103 33
Size: 995 191 1121
Fragments arranged by size:
1158 1121 995 685 432 301 191 161
77 61 9
SfoI GGC'GCC
Cuts at: 1536 2621 2869 1536
Size: 1085 248 3858
Fragments arranged by size:
3858 1085 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
SmaI CCC'GGG
Cuts at: 2211 2211
Size: 5191
SmlI C'TyrA_G
Cuts at: 1288 1726 2344 3259 3753 4015 1288
Size: 438 618 915 494 262 2464
Fragments arranged by size:
2464 915 618 494 438 262
SnaBI TAC'GTA
Cuts at: 2181 2181
Size: 5191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5191
SphI G_CATG'C
Cuts at: 2242 2742 2242
Size: 500 4691
StuI AGG'CCT
Cuts at: 1987 1987
Size: 5191
StyI C'CwwG_G
Cuts at: 1373 1664 2323 2677 1373
Size: 291 659 354 3887
Fragments arranged by size:
3887 659 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 2772 2905 3364 3575 1069
Size: 1703 133 459 211 2685
Fragments arranged by size:
2685 1703 459 211 133
TatI w'GTAC_w
Cuts at: 1442 2114 4706 1442
Size: 672 2592 1927
Fragments arranged by size:
2592 1927 672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1234 1266 4708 4740 1234
Size: 32 3442 32 1685
Fragments arranged by size:
3442 1685 32 32
Tth111I GACn'n_nGTC
Cuts at: 198 366 382 2986 198
Size: 168 16 2604 2403
Fragments arranged by size:
2604 2403 168 16
XbaI T'CTAG_A
Cuts at: 984 2220 984
Size: 1236 3955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 2684 447
Size: 2237 2954
XmaI C'CCGG_G
Cuts at: 2209 2209
Size: 5191
XmnI GAAnn'nnTTC
Cuts at: 1153 1153
Size: 5191
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PsiI PspXI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI