Restriction map for
pF1KSDA1072
(Circular) (Six-base) MAPSORT of: pF1KSDA1072.seq Check: 2296 from: 1 to: 5410
pF1KSDA1072 5410 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2446 2446
Size: 5410
Acc65I G'GTAC_C
Cuts at: 2424 2424
Size: 5410
AclI AA'CG_TT
Cuts at: 5250 5250
Size: 5410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 732 3233 3665 4414 4682 637
Size: 95 2501 432 749 268 1365
Fragments arranged by size:
2501 1365 749 432 268 95
AflII C'TTAA_G
Cuts at: 1107 1107
Size: 5410
AflIII A'CryG_T
Cuts at: 873 1158 2760 3866 4843 873
Size: 285 1602 1106 977 1440
Fragments arranged by size:
1602 1440 1106 977 285
AgeI A'CCGG_T
Cuts at: 3798 3798
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1126 1126
Size: 5410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2268 2300 2268
Size: 32 5378
AlwNI CAG_nnn'CTG
Cuts at: 1384 2713 4282 1384
Size: 1329 1569 2512
Fragments arranged by size:
2512 1569 1329
ApaLI G'TGCA_C
Cuts at: 1916 4180 1916
Size: 2264 3146
ApoI r'AATT_y
Cuts at: 2412 4763 5019 2412
Size: 2351 256 2803
Fragments arranged by size:
2803 2351 256
AseI AT'TA_AT
Cuts at: 20 1940 20
Size: 1920 3490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5410
AvaI C'yCGr_G
Cuts at: 259 271 328 391 456 2109 2428 259
Size: 12 57 63 65 1653 319 3241
Fragments arranged by size:
3241 1653 319 65 63 57 12
AvrII C'CTAG_G
Cuts at: 1749 1749
Size: 5410
BamHI G'GATC_C
Cuts at: 2433 2433
Size: 5410
BanI G'GyrC_C
Cuts at: 1297 2424 2838 3086 3121 1297
Size: 1127 414 248 35 3586
Fragments arranged by size:
3586 1127 414 248 35
BanII G_rGCy'C
Cuts at: 272 278 392 1801 2422 3452 272
Size: 6 114 1409 621 1030 2230
Fragments arranged by size:
2230 1409 1030 621 114 6
BbeI G_GCGC'C
Cuts at: 2842 3090 2842
Size: 248 5162
BbsI GAAGACnn'nnnn_
Cuts at: 738 1418 1832 738
Size: 680 414 4316
Fragments arranged by size:
4316 680 414
BbvCI CC'TCA_GC
Cuts at: 299 1040 1721 299
Size: 741 681 3988
Fragments arranged by size:
3988 741 681
BciVI GTATCCnnnnn_n'
Cuts at: 3306 4069 5410 3306
Size: 763 1341 3306
Fragments arranged by size:
3306 1341 763
BclI T'GATC_A
Cuts at: 2928 2928
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 2298 2298
Size: 5410
BglII A'GATC_T
Cuts at: 1842 2370 2923 4962 1842
Size: 528 553 2039 2290
Fragments arranged by size:
2290 2039 553 528
BlpI GC'TnA_GC
Cuts at: 1734 2520 1734
Size: 786 4624
Bme1580I G_kGCm'C
Cuts at: 464 1881 1920 3033 3126 4184 464
Size: 1417 39 1113 93 1058 1690
Fragments arranged by size:
1690 1417 1113 1058 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 1875 2620 2701 3036 5182 1875
Size: 745 81 335 2146 2103
Fragments arranged by size:
2146 2103 745 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 3775 2084
Size: 1691 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1323 2021 190
Size: 1133 698 3579
Fragments arranged by size:
3579 1133 698
Bpu10I CC'TnA_GC
Cuts at: 299 1040 1721 1795 299
Size: 741 681 74 3914
Fragments arranged by size:
3914 741 681 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2584 3463 3957 4255 2195
Size: 389 879 494 298 3350
Fragments arranged by size:
3350 879 494 389 298
BsaI GGTCTCn'nnnn_
Cuts at: 798 5114 798
Size: 4316 1094
BsaAI yAC'GTr
Cuts at: 2400 2761 2400
Size: 361 5049
BsaBI GATnn'nnATC
Cuts at: 6 2922 4961 6
Size: 2916 2039 455
Fragments arranged by size:
2916 2039 455
BsaHI Gr'CG_yC
Cuts at: 2839 3087 3791 5287 2839
Size: 248 704 1496 2962
Fragments arranged by size:
2962 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2597 3118 3798 4072 4219 4630 2597
Size: 521 680 274 147 411 3377
Fragments arranged by size:
3377 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1268 1298 1268
Size: 30 5380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 349 376 409 412 433 436 439 442
Size: 189 27 33 3 21 3 3 3
Cuts at: 442 559 160
Size: 117 5011
Fragments arranged by size:
5011 189 117 33 27 21 3 3
3 3
BseYI C'CCAG_C
Cuts at: 488 776 809 1555 2833 3591 4170 4666 488
Size: 288 33 746 1278 758 579 496 1232
Fragments arranged by size:
1278 1232 758 746 579 496 288 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1071 1105 653
Size: 418 34 4958
Fragments arranged by size:
4958 418 34
BsiEI CG_ry'CG
Cuts at: 104 308 470 520 2950 2996 4206 104
Size: 204 162 50 2430 46 1210 1308
Fragments arranged by size:
2430 1308 1210 204 162 50 46
BsiHKAI G_wGCw'C
Cuts at: 1735 1920 2422 3200 3390 4184 1735
Size: 185 502 778 190 794 2961
Fragments arranged by size:
2961 794 778 502 190 185
BsmI GAATG_Cn'
Cuts at: 881 2060 881
Size: 1179 4231
Bsp1286I G_dGCh'C
Cuts at: 272 278 392 464 1735 1801 1881 1920 2422
Size: 6 114 72 1271 66 80 39 502
Cuts at: 2422 3033 3126 3200 3390 3452 4184 272
Size: 611 93 74 190 62 732 1498
Fragments arranged by size:
1498 1271 732 611 502 190 114 93
80 74 72 66 62 39 6
BspEI T'CCGG_A
Cuts at: 4630 4630
Size: 5410
BspHI T'CATG_A
Cuts at: 2352 4586 2352
Size: 2234 3176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2458 2672 2974 3355 2458
Size: 214 302 381 4513
Fragments arranged by size:
4513 381 302 214
BsrBI CCG'CTC
Cuts at: 50 510 1077 3700 3754 5240 5405 50
Size: 460 567 2623 54 1486 165 55
Fragments arranged by size:
2623 1486 567 460 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1978 3320 1978
Size: 1342 4068
BsrFI r'CCGG_y
Cuts at: 130 158 3406 3587 3798 130
Size: 28 3248 181 211 1742
Fragments arranged by size:
3248 1742 211 181 28
BssSI C'ACGA_G
Cuts at: 760 1547 3679 4039 760
Size: 787 2132 360 2131
Fragments arranged by size:
2132 2131 787 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 2785 1384
Size: 1401 4009
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 1128 1398 1128
Size: 270 5140
BstYI r'GATC_y
Cuts at: 1 1842 2370 2433 2900 2923 3258 3504 4518
Size: 1841 528 63 467 23 335 246 1014
Cuts at: 4518 4604 4616 4962 1
Size: 86 12 346 449
Fragments arranged by size:
1841 1014 528 467 449 346 335 246
86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 1594 143
Size: 1451 3959
BtgI C'CryG_G
Cuts at: 125 344 954 2293 125
Size: 219 610 1339 3242
Fragments arranged by size:
3242 1339 610 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3358 3539 3702 3358
Size: 181 163 5066
Fragments arranged by size:
5066 181 163
BtsI GCAGTG_nn'
Cuts at: 947 1475 2012 2651 947
Size: 528 537 639 3706
Fragments arranged by size:
3706 639 537 528
DraI TTT'AAA
Cuts at: 2407 2407
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 874 3114 3974 874
Size: 2240 860 2310
Fragments arranged by size:
2310 2240 860
EaeI y'GGCC_r
Cuts at: 128 305 341 467 951 2993 3167 3558 3585
Size: 177 36 126 484 2042 174 391 27
Cuts at: 3585 4932 128
Size: 1347 606
Fragments arranged by size:
2042 1347 606 484 391 177 174 126
36 27
EagI C'GGCC_G
Cuts at: 305 467 2993 305
Size: 162 2526 2722
Fragments arranged by size:
2722 2526 162
EarI CTCTTCn'nnn_
Cuts at: 907 2260 3431 3641 907
Size: 1353 1171 210 2676
Fragments arranged by size:
2676 1353 1171 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1000 3616 3928 4074 5220 1000
Size: 2616 312 146 1146 1190
Fragments arranged by size:
2616 1190 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 637 732 1323 2021 3233 3665 4414 4682
Size: 447 95 591 698 1212 432 749 268
Cuts at: 4682 190
Size: 918
Fragments arranged by size:
1212 918 749 698 591 447 432 268
95
EcoO109I rG'GnC_Cy
Cuts at: 1044 1055 1269 1805 2547 1044
Size: 11 214 536 742 3907
Fragments arranged by size:
3907 742 536 214 11
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1951 3427 1951
Size: 1476 3934
FspI TGC'GCA
Cuts at: 853 3189 853
Size: 2336 3074
HaeII r_GCGC'y
Cuts at: 165 2842 3090 4114 5093 165
Size: 2677 248 1024 979 482
Fragments arranged by size:
2677 1024 979 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1372 1404 1543 1575 1893 1925 1948 1980 3250
Size: 32 139 32 318 32 23 32 1270
Cuts at: 3250 3280 3282 3312 1372
Size: 30 2 30 3470
Fragments arranged by size:
3470 1270 318 139 32 32 32 32
30 30 23 2
HincII GTy'rAC
Cuts at: 1002 2447 1002
Size: 1445 3965
HindIII A'AGCT_T
Cuts at: 929 929
Size: 5410
HpaI GTT'AAC
Cuts at: 1002 1002
Size: 5410
KasI G'GCGC_C
Cuts at: 2838 3086 2838
Size: 248 5162
KpnI G_GTAC'C
Cuts at: 2428 2428
Size: 5410
MluI A'CGCG_T
Cuts at: 4843 4843
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1508 2066 2290 4081 4265 1508
Size: 558 224 1791 184 2653
Fragments arranged by size:
2653 1791 558 224 184
MscI TGG'CCA
Cuts at: 953 3169 953
Size: 2216 3194
MslI CAynn'nnrTG
Cuts at: 1126 3397 3524 1126
Size: 2271 127 3012
Fragments arranged by size:
3012 2271 127
MspA1I CmG'CkG
Cuts at: 492 522 776 2519 2833 3193 4208 4453 492
Size: 30 254 1743 314 360 1015 245 1449
Fragments arranged by size:
1743 1449 1015 360 314 254 245 30
NaeI GCC'GGC
Cuts at: 132 3589 132
Size: 3457 1953
NarI GG'CG_CC
Cuts at: 2839 3087 2839
Size: 248 5162
NcoI C'CATG_G
Cuts at: 125 954 2293 125
Size: 829 1339 3242
Fragments arranged by size:
3242 1339 829
NgoMIV G'CCGG_C
Cuts at: 130 3587 130
Size: 3457 1953
NruI TCG'CGA
Cuts at: 3771 3771
Size: 5410
NspI r_CATG'y
Cuts at: 852 877 1155 1162 2461 2961 3870 852
Size: 25 278 7 1299 500 909 2392
Fragments arranged by size:
2392 1299 909 500 278 25 7
PasI CC'CwG_GG
Cuts at: 1801 1801
Size: 5410
PciI A'CATG_T
Cuts at: 873 1158 3866 873
Size: 285 2708 2417
Fragments arranged by size:
2708 2417 285
PfoI T'CCnGG_A
Cuts at: 167 167
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4549 4581 4549
Size: 32 5378
PpuMI rG'GwC_Cy
Cuts at: 1805 1805
Size: 5410
PshAI GACnn'nnGTC
Cuts at: 522 522
Size: 5410
PstI C_TGCA'G
Cuts at: 581 775 2455 3140 581
Size: 194 1680 685 2851
Fragments arranged by size:
2851 1680 685 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5410
PvuII CAG'CTG
Cuts at: 492 776 2833 3193 492
Size: 284 2057 360 2709
Fragments arranged by size:
2709 2057 360 284
RsrII CG'GwC_CG
Cuts at: 196 3603 196
Size: 3407 2003
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5410
SalI G'TCGA_C
Cuts at: 2445 2445
Size: 5410
SanDI GG'GwC_CC
Cuts at: 1805 1805
Size: 5410
SapI GCTCTTCn'nnn_
Cuts at: 907 3431 3641 907
Size: 2524 210 2676
Fragments arranged by size:
2676 2524 210
SbfI CC_TGCA'GG
Cuts at: 2455 2455
Size: 5410
ScaI AGT'ACT
Cuts at: 4927 4927
Size: 5410
SfcI C'TryA_G
Cuts at: 33 577 771 2451 3136 4131 4322 33
Size: 544 194 1680 685 995 191 1121
Fragments arranged by size:
1680 1121 995 685 544 194 191
SfoI GGC'GCC
Cuts at: 2840 3088 2840
Size: 248 5162
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5410
SmaI CCC'GGG
Cuts at: 330 458 2111 2430 330
Size: 128 1653 319 3310
Fragments arranged by size:
3310 1653 319 128
SmlI C'TyrA_G
Cuts at: 1107 2210 2563 3478 3972 4234 1107
Size: 1103 353 915 494 262 2283
Fragments arranged by size:
2283 1103 915 494 353 262
SnaBI TAC'GTA
Cuts at: 2400 2400
Size: 5410
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5410
SphI G_CATG'C
Cuts at: 2461 2961 2461
Size: 500 4910
StyI C'CwwG_G
Cuts at: 125 954 1272 1714 1749 2293 2377 2542 2896
Size: 829 318 442 35 544 84 165 354
Cuts at: 2896 125
Size: 2639
Fragments arranged by size:
2639 829 544 442 354 318 165 84
35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 1158 1162 1423 2991 3124 3583 3794 768
Size: 390 4 261 1568 133 459 211 2384
Fragments arranged by size:
2384 1568 459 390 261 211 133 4
TatI w'GTAC_w
Cuts at: 4925 4925
Size: 5410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 4927 4959 662
Size: 32 4233 32 1113
Fragments arranged by size:
4233 1113 32 32
Tth111I GACn'n_nGTC
Cuts at: 3205 3205
Size: 5410
XbaI T'CTAG_A
Cuts at: 2125 2439 2125
Size: 314 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2903 2375
Size: 528 4882
XmaI C'CCGG_G
Cuts at: 328 456 2109 2428 328
Size: 128 1653 319 3310
Fragments arranged by size:
3310 1653 319 128
XmnI GAAnn'nnTTC
Cuts at: 650 787 650
Size: 137 5273
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI MfeI NdeI NheI NotI NsiI PacI PflMI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI