Restriction map for
pF1KSDA1075
(Circular) (Six-base) MAPSORT of: pF1KSDA1075.seq Check: 5836 from: 1 to: 7366
pF1KSDA1075 7366 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 605 1297 3297 3368 605
Size: 692 2000 71 4603
Fragments arranged by size:
4603 2000 692 71
AccI GT'mk_AC
Cuts at: 1127 1451 4402 1127
Size: 324 2951 4091
Fragments arranged by size:
4091 2951 324
Acc65I G'GTAC_C
Cuts at: 2471 2693 4380 2471
Size: 222 1687 5457
Fragments arranged by size:
5457 1687 222
AclI AA'CG_TT
Cuts at: 7206 7206
Size: 7366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 465 969 2410 3607 5189 5621 6370 6638
Size: 127 504 1441 1197 1582 432 749 268
Cuts at: 6638 338
Size: 1066
Fragments arranged by size:
1582 1441 1197 1066 749 504 432 268
127
AflIII A'CryG_T
Cuts at: 1225 4716 5822 6799 1225
Size: 3491 1106 977 1792
Fragments arranged by size:
3491 1792 1106 977
AgeI A'CCGG_T
Cuts at: 5754 5754
Size: 7366
AhdI GACnn_n'nnGTC
Cuts at: 2913 2913
Size: 7366
AleI CACnn'nnGTG
Cuts at: 529 1238 3226 529
Size: 709 1988 4669
Fragments arranged by size:
4669 1988 709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 484 2378 484
Size: 1894 5472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2492 2524 3704 3736 2492
Size: 32 1180 32 6122
Fragments arranged by size:
6122 1180 32 32
AlwNI CAG_nnn'CTG
Cuts at: 311 900 1159 1732 1746 2113 2519 2943 3080
Size: 589 259 573 14 367 406 424 137
Cuts at: 3080 4669 6238 311
Size: 1589 1569 1439
Fragments arranged by size:
1589 1569 1439 589 573 424 406 367
259 137 14
ApaI G_GGCC'C
Cuts at: 2776 3397 3749 4000 2776
Size: 621 352 251 6142
Fragments arranged by size:
6142 621 352 251
ApaLI G'TGCA_C
Cuts at: 6136 6136
Size: 7366
ApoI r'AATT_y
Cuts at: 922 4368 6719 6975 922
Size: 3446 2351 256 1313
Fragments arranged by size:
3446 2351 1313 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7366
AvaI C'yCGr_G
Cuts at: 1402 1547 2622 2807 3185 3364 4013 4384 1402
Size: 145 1075 185 378 179 649 371 4384
Fragments arranged by size:
4384 1075 649 378 371 185 179 145
AvrII C'CTAG_G
Cuts at: 202 1758 202
Size: 1556 5810
BamHI G'GATC_C
Cuts at: 427 2008 4389 427
Size: 1581 2381 3404
Fragments arranged by size:
3404 2381 1581
BanI G'GyrC_C
Cuts at: 584 1611 2048 2321 2471 2590 2693 2708 2983
Size: 1027 437 273 150 119 103 15 275
Cuts at: 2983 3118 4306 4380 4794 5042 5077 584
Size: 135 1188 74 414 248 35 2873
Fragments arranged by size:
2873 1188 1027 437 414 275 273 248
150 135 119 103 74 35 15
BanII G_rGCy'C
Cuts at: 165 1928 2776 3136 3280 3397 3749 3785 4000
Size: 1763 848 360 144 117 352 36 215
Cuts at: 4000 4378 5408 165
Size: 378 1030 2123
Fragments arranged by size:
2123 1763 1030 848 378 360 352 215
144 117 36
BbeI G_GCGC'C
Cuts at: 2052 3122 4310 4798 5046 2052
Size: 1070 1188 488 248 4372
Fragments arranged by size:
4372 1188 1070 488 248
BbsI GAAGACnn'nnnn_
Cuts at: 1380 1380
Size: 7366
BbvCI CC'TCA_GC
Cuts at: 1888 2718 2925 1888
Size: 830 207 6329
Fragments arranged by size:
6329 830 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3166 3200 3166
Size: 34 7332
BciVI GTATCCnnnnn_n'
Cuts at: 1877 2588 3522 5262 6025 7366 1877
Size: 711 934 1740 763 1341 1877
Fragments arranged by size:
1877 1740 1341 934 763 711
BclI T'GATC_A
Cuts at: 3614 4884 3614
Size: 1270 6096
BglI GCCn_nnn'nGGC
Cuts at: 364 1596 1913 2450 3188 364
Size: 1232 317 537 738 4542
Fragments arranged by size:
4542 1232 738 537 317
BglII A'GATC_T
Cuts at: 875 4217 4879 6918 875
Size: 3342 662 2039 1323
Fragments arranged by size:
3342 2039 1323 662
BlpI GC'TnA_GC
Cuts at: 212 2656 4476 212
Size: 2444 1820 3102
Fragments arranged by size:
3102 2444 1820
Bme1580I G_kGCm'C
Cuts at: 1252 2324 2420 2446 2595 2711 2776 2786 3143
Size: 1072 96 26 149 116 65 10 357
Cuts at: 3143 3397 3687 3749 3905 4000 4989 5082 6140
Size: 254 290 62 156 95 989 93 1058
Cuts at: 6140 1252
Size: 2478
Fragments arranged by size:
2478 1072 1058 989 357 290 254 156
149 116 96 95 93 65 62 26
10
BmrI ACTGGGnnnn_n'
Cuts at: 397 2123 2302 2507 2771 3037 3068 3674 3752
Size: 1726 179 205 264 266 31 606 78
Cuts at: 3752 3770 4348 4576 4657 4992 7138 397
Size: 18 578 228 81 335 2146 625
Fragments arranged by size:
2146 1726 625 606 578 335 266 264
228 205 179 81 78 31 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2445 5731 2445
Size: 3286 4080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1157 1207 1380 1604 1993 2182 2272 2285
Size: 918 50 173 224 389 189 90 13
Cuts at: 2285 2543 2770 2784 3881 4146 239
Size: 258 227 14 1097 265 3459
Fragments arranged by size:
3459 1097 918 389 265 258 227 224
189 173 90 50 14 13
Bpu10I CC'TnA_GC
Cuts at: 719 1888 2718 2925 2999 3023 3328 719
Size: 1169 830 207 74 24 305 4757
Fragments arranged by size:
4757 1169 830 305 207 74 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4160 4540 5419 5913 6211 3643
Size: 517 380 879 494 298 4798
Fragments arranged by size:
4798 879 517 494 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 1449 2807 3218 3816 3877 7070 1449
Size: 1358 411 598 61 3193 1745
Fragments arranged by size:
3193 1745 1358 598 411 61
BsaAI yAC'GTr
Cuts at: 531 801 4356 4717 531
Size: 270 3555 361 3180
Fragments arranged by size:
3555 3180 361 270
BsaBI GATnn'nnATC
Cuts at: 6 4878 6917 6
Size: 4872 2039 455
Fragments arranged by size:
4872 2039 455
BsaHI Gr'CG_yC
Cuts at: 2049 3119 4307 4795 5043 5747 7243 2049
Size: 1070 1188 488 248 704 1496 2172
Fragments arranged by size:
2172 1496 1188 1070 704 488 248
BsaWI w'CCGG_w
Cuts at: 242 1256 2986 4181 4553 5074 5754 6028 6175
Size: 1014 1730 1195 372 521 680 274 147
Cuts at: 6175 6586 242
Size: 411 1022
Fragments arranged by size:
1730 1195 1022 1014 680 521 411 372
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2492 2522 2743 2773 3045 3075 3811 3841 3970
Size: 30 221 30 272 30 736 30 129
Cuts at: 3970 4000 4249 4279 2492
Size: 30 249 30 5579
Fragments arranged by size:
5579 736 272 249 221 129 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 1189 1192 1383 1992 2221 2516 2692 186
Size: 1003 3 191 609 229 295 176 4860
Fragments arranged by size:
4860 1003 609 295 229 191 176 3
BseYI C'CCAG_C
Cuts at: 509 962 1166 1481 1795 2196 2523 2581 3064
Size: 453 204 315 314 401 327 58 483
Cuts at: 3064 3686 3815 4789 5547 6126 6622 509
Size: 622 129 974 758 579 496 1253
Fragments arranged by size:
1253 974 758 622 579 496 483 453
401 327 315 314 204 129 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 324 1021 1275 1603 2287 2845 284
Size: 40 697 254 328 684 558 4805
Fragments arranged by size:
4805 697 684 558 328 254 40
BsiEI CG_ry'CG
Cuts at: 104 1717 2062 4906 4952 6162 104
Size: 1613 345 2844 46 1210 1308
Fragments arranged by size:
2844 1613 1308 1210 345 46
BsiHKAI G_wGCw'C
Cuts at: 422 754 1049 3280 3919 3962 4378 5156 5346
Size: 332 295 2231 639 43 416 778 190
Cuts at: 5346 6140 422
Size: 794 1648
Fragments arranged by size:
2231 1648 794 778 639 416 332 295
190 43
BsmI GAATG_Cn'
Cuts at: 3857 3857
Size: 7366
BsmBI CGTCTCn'nnnn_
Cuts at: 1798 1798
Size: 7366
Bsp1286I G_dGCh'C
Cuts at: 165 422 754 1049 1252 1928 2324 2420 2446
Size: 257 332 295 203 676 396 96 26
Cuts at: 2446 2595 2711 2776 2786 3136 3143 3280 3397
Size: 149 116 65 10 350 7 137 117
Cuts at: 3397 3687 3749 3785 3905 3919 3962 4000 4378
Size: 290 62 36 120 14 43 38 378
Cuts at: 4378 4989 5082 5156 5346 5408 6140 165
Size: 611 93 74 190 62 732 1391
Fragments arranged by size:
1391 732 676 611 396 378 350 332
295 290 257 203 190 149 137 120
117 116 96 93 74 65 62 62
43 38 36 26 14 10 7
BspEI T'CCGG_A
Cuts at: 242 6586 242
Size: 6344 1022
BspHI T'CATG_A
Cuts at: 6542 6542
Size: 7366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 605 653 1297 2444 3297 3368 4414 4628 4930
Size: 48 644 1147 853 71 1046 214 302
Cuts at: 4930 5311 605
Size: 381 2660
Fragments arranged by size:
2660 1147 1046 853 644 381 302 214
71 48
BsrBI CCG'CTC
Cuts at: 50 495 504 5656 5710 7196 7361 50
Size: 445 9 5152 54 1486 165 55
Fragments arranged by size:
5152 1486 445 165 55 54 9
BsrDI GCAATG_nn'
Cuts at: 1148 2698 3516 3575 5276 1148
Size: 1550 818 59 1701 3238
Fragments arranged by size:
3238 1701 1550 818 59
BsrFI r'CCGG_y
Cuts at: 1692 1712 1955 2754 5362 5543 5754 1692
Size: 20 243 799 2608 181 211 3304
Fragments arranged by size:
3304 2608 799 243 211 181 20
BssSI C'ACGA_G
Cuts at: 1264 1513 2419 5635 5995 1264
Size: 249 906 3216 360 2635
Fragments arranged by size:
3216 2635 906 360 249
BstAPI GCAn_nnn'nTGC
Cuts at: 311 1044 2310 2328 4741 311
Size: 733 1266 18 2413 2936
Fragments arranged by size:
2936 2413 1266 733 18
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7366
BstEII G'GTnAC_C
Cuts at: 276 276
Size: 7366
BstXI CCAn_nnnn'nTGG
Cuts at: 1882 3115 3389 1882
Size: 1233 274 5859
Fragments arranged by size:
5859 1233 274
BstYI r'GATC_y
Cuts at: 1 427 875 2008 2666 3870 4217 4389 4856
Size: 426 448 1133 658 1204 347 172 467
Cuts at: 4856 4879 5214 5460 6474 6560 6572 6918 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1204 1133 1014 658 467 449 448 426
347 346 335 246 172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3086 3086
Size: 7366
BtgI C'CryG_G
Cuts at: 377 767 1299 1376 1426 2602 3707 377
Size: 390 532 77 50 1176 1105 4036
Fragments arranged by size:
4036 1176 1105 532 390 77 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1226 3395 5314 5495 5658 1226
Size: 2169 1919 181 163 2934
Fragments arranged by size:
2934 2169 1919 181 163
BtsI GCAGTG_nn'
Cuts at: 1896 2791 2829 4607 1896
Size: 895 38 1778 4655
Fragments arranged by size:
4655 1778 895 38
ClaI AT'CG_AT
Cuts at: 284 284
Size: 7366
DraI TTT'AAA
Cuts at: 4363 4363
Size: 7366
DraIII CAC_nnn'GTG
Cuts at: 1044 2310 2441 3682 1044
Size: 1266 131 1241 4728
Fragments arranged by size:
4728 1266 1241 131
DrdI GACnn_nn'nnGTC
Cuts at: 1441 5070 5930 1441
Size: 3629 860 2877
Fragments arranged by size:
3629 2877 860
EaeI y'GGCC_r
Cuts at: 548 902 941 1242 1710 1714 1775 1808 1957
Size: 354 39 301 468 4 61 33 149
Cuts at: 1957 2532 3254 3665 4232 4949 5123 5514 5541
Size: 575 722 411 567 717 174 391 27
Cuts at: 5541 6888 548
Size: 1347 1026
Fragments arranged by size:
1347 1026 722 717 575 567 468 411
391 354 301 174 149 61 39 33
27 4
EagI C'GGCC_G
Cuts at: 1714 4949 1714
Size: 3235 4131
EarI CTCTTCn'nnn_
Cuts at: 655 1036 1820 2038 3875 4126 5387 5597 655
Size: 381 784 218 1837 251 1261 210 2424
Fragments arranged by size:
2424 1837 1261 784 381 251 218 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 904 1859 3151 3712 3792 5572 5884 6030 7176
Size: 955 1292 561 80 1780 312 146 1146
Cuts at: 7176 904
Size: 1094
Fragments arranged by size:
1780 1292 1146 1094 955 561 312 146
80
EcoICRI GAG'CTC
Cuts at: 3278 4376 3278
Size: 1098 6268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 338 465 969 1157 1207 1380 1604 1993
Size: 99 127 504 188 50 173 224 389
Cuts at: 1993 2182 2272 2285 2410 2543 2770 2784 3607
Size: 189 90 13 125 133 227 14 823
Cuts at: 3607 3881 4146 5189 5621 6370 6638 239
Size: 274 265 1043 432 749 268 967
Fragments arranged by size:
1043 967 823 749 504 432 389 274
268 265 227 224 189 188 173 133
127 125 99 90 50 14 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1079 3586 3937 1079
Size: 2507 351 4508
Fragments arranged by size:
4508 2507 351
EcoO109I rG'GnC_Cy
Cuts at: 1156 1552 1849 2166 2725 2772 2773 2915 2994
Size: 396 297 317 559 47 1 142 79
Cuts at: 2994 3181 3190 3385 3393 3597 3605 3702 3997
Size: 187 9 195 8 204 8 97 295
Cuts at: 3997 4503 1156
Size: 506 4019
Fragments arranged by size:
4019 559 506 396 317 297 295 204
195 187 142 97 79 47 9 8
8 1
EcoRI G'AATT_C
Cuts at: 4368 4368
Size: 7366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4140 5383 4140
Size: 1243 6123
FseI GG_CCGG'CC
Cuts at: 1716 1716
Size: 7366
FspI TGC'GCA
Cuts at: 3856 5145 3856
Size: 1289 6077
HaeII r_GCGC'y
Cuts at: 510 2052 2221 2932 3122 4310 4798 5046 6070
Size: 1542 169 711 190 1188 488 248 1024
Cuts at: 6070 7049 510
Size: 979 827
Fragments arranged by size:
1542 1188 1024 979 827 711 488 248
190 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1295 1327 2743 2775 2899 2931 2963 2992 3024
Size: 32 1416 32 124 32 32 29 32
Cuts at: 3024 3045 3077 3391 3423 3970 4002 5206 5236
Size: 21 32 314 32 547 32 1204 30
Cuts at: 5236 5238 5268 1295
Size: 2 30 3393
Fragments arranged by size:
3393 1416 1204 547 314 124 32 32
32 32 32 32 32 32 30 30
29 21 2
HincII GTy'rAC
Cuts at: 1452 4403 1452
Size: 2951 4415
HindIII A'AGCT_T
Cuts at: 835 835
Size: 7366
KasI G'GCGC_C
Cuts at: 2048 3118 4306 4794 5042 2048
Size: 1070 1188 488 248 4372
Fragments arranged by size:
4372 1188 1070 488 248
KpnI G_GTAC'C
Cuts at: 2475 2697 4384 2475
Size: 222 1687 5457
Fragments arranged by size:
5457 1687 222
MluI A'CGCG_T
Cuts at: 6799 6799
Size: 7366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3076 6037 6221 3076
Size: 2961 184 4221
Fragments arranged by size:
4221 2961 184
MscI TGG'CCA
Cuts at: 904 943 1777 3256 3667 4234 5125 904
Size: 39 834 1479 411 567 891 3145
Fragments arranged by size:
3145 1479 891 834 567 411 39
MslI CAynn'nnrTG
Cuts at: 529 1012 1054 1238 2360 2549 3113 3226 3910
Size: 483 42 184 1122 189 564 113 684
Cuts at: 3910 5353 5480 529
Size: 1443 127 2415
Fragments arranged by size:
2415 1443 1122 684 564 483 189 184
127 113 42
MspA1I CmG'CkG
Cuts at: 137 582 1587 1729 2328 2384 3717 3922 4060
Size: 445 1005 142 599 56 1333 205 138
Cuts at: 4060 4475 4789 5149 6164 6409 137
Size: 415 314 360 1015 245 1094
Fragments arranged by size:
1333 1094 1015 1005 599 445 415 360
314 245 205 142 138 56
NaeI GCC'GGC
Cuts at: 1694 1714 5545 1694
Size: 20 3831 3515
Fragments arranged by size:
3831 3515 20
NarI GG'CG_CC
Cuts at: 2049 3119 4307 4795 5043 2049
Size: 1070 1188 488 248 4372
Fragments arranged by size:
4372 1188 1070 488 248
NcoI C'CATG_G
Cuts at: 767 767
Size: 7366
NdeI CA'TA_TG
Cuts at: 2529 2529
Size: 7366
NgoMIV G'CCGG_C
Cuts at: 1692 1712 5543 1692
Size: 20 3831 3515
Fragments arranged by size:
3831 3515 20
NruI TCG'CGA
Cuts at: 5727 5727
Size: 7366
NspI r_CATG'y
Cuts at: 730 864 1229 4417 4917 5826 730
Size: 134 365 3188 500 909 2270
Fragments arranged by size:
3188 2270 909 500 365 134
PasI CC'CwG_GG
Cuts at: 165 2016 2170 3389 3601 4086 4253 165
Size: 1851 154 1219 212 485 167 3278
Fragments arranged by size:
3278 1851 1219 485 212 167 154
PciI A'CATG_T
Cuts at: 1225 5822 1225
Size: 4597 2769
PflMI CCAn_nnn'nTGG
Cuts at: 900 2113 2530 2608 2704 3080 3542 4008 900
Size: 1213 417 78 96 376 462 466 4258
Fragments arranged by size:
4258 1213 466 462 417 376 96 78
PfoI T'CCnGG_A
Cuts at: 227 2159 2559 2761 3524 227
Size: 1932 400 202 763 4069
Fragments arranged by size:
4069 1932 763 400 202
PmeI GTTT'AAAC
Cuts at: 4363 4363
Size: 7366
PmlI CAC'GTG
Cuts at: 801 801
Size: 7366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1423 1455 2492 2524 6505 6537 1423
Size: 32 1037 32 3981 32 2252
Fragments arranged by size:
3981 2252 1037 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1552 2166 2725 2915 3385 3597 3702 1552
Size: 614 559 190 470 212 105 5216
Fragments arranged by size:
5216 614 559 470 212 190 105
PspOMI G'GGCC_C
Cuts at: 2772 3393 3745 3996 2772
Size: 621 352 251 6142
Fragments arranged by size:
6142 621 352 251
PspXI vC'TCGA_Gb
Cuts at: 3185 3185
Size: 7366
PstI C_TGCA'G
Cuts at: 885 956 2742 3955 4029 4411 5096 885
Size: 71 1786 1213 74 382 685 3155
Fragments arranged by size:
3155 1786 1213 685 382 74 71
PvuI CG_AT'CG
Cuts at: 104 2062 104
Size: 1958 5408
PvuII CAG'CTG
Cuts at: 1729 2384 3717 4060 4789 5149 1729
Size: 655 1333 343 729 360 3946
Fragments arranged by size:
3946 1333 729 655 360 343
RsrII CG'GwC_CG
Cuts at: 5559 5559
Size: 7366
SacI G_AGCT'C
Cuts at: 3280 4378 3280
Size: 1098 6268
SalI G'TCGA_C
Cuts at: 1450 4401 1450
Size: 2951 4415
SanDI GG'GwC_CC
Cuts at: 1552 3702 1552
Size: 2150 5216
SapI GCTCTTCn'nnn_
Cuts at: 655 1820 4126 5387 5597 655
Size: 1165 2306 1261 210 2424
Fragments arranged by size:
2424 2306 1261 1165 210
SbfI CC_TGCA'GG
Cuts at: 4411 4411
Size: 7366
ScaI AGT'ACT
Cuts at: 6883 6883
Size: 7366
SexAI A'CCwGG_T
Cuts at: 2911 2911
Size: 7366
SfcI C'TryA_G
Cuts at: 33 867 881 952 1724 2225 2379 2738 3951
Size: 834 14 71 772 501 154 359 1213
Cuts at: 3951 4025 4038 4407 5092 6087 6278 33
Size: 74 13 369 685 995 191 1121
Fragments arranged by size:
1213 1121 995 834 772 685 501 369
359 191 154 74 71 14 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 3188 3188
Size: 7366
SfoI GGC'GCC
Cuts at: 2050 3120 4308 4796 5044 2050
Size: 1070 1188 488 248 4372
Fragments arranged by size:
4372 1188 1070 488 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7366
SmaI CCC'GGG
Cuts at: 1549 2624 3366 4015 4386 1549
Size: 1075 742 649 371 4529
Fragments arranged by size:
4529 1075 742 649 371
SmlI C'TyrA_G
Cuts at: 3185 3658 4175 4519 5434 5928 6190 3185
Size: 473 517 344 915 494 262 4361
Fragments arranged by size:
4361 915 517 494 473 344 262
SnaBI TAC'GTA
Cuts at: 4356 4356
Size: 7366
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7366
SphI G_CATG'C
Cuts at: 730 4417 4917 730
Size: 3687 500 3179
Fragments arranged by size:
3687 3179 500
SrfI GCCC'GGGC
Cuts at: 3366 4015 3366
Size: 649 6717
StuI AGG'CCT
Cuts at: 201 367 1342 1881 2032 2959 201
Size: 166 975 539 151 927 4608
Fragments arranged by size:
4608 975 927 539 166 151
StyI C'CwwG_G
Cuts at: 202 695 767 1322 1758 2399 3636 3806 4498
Size: 493 72 555 436 641 1237 170 692
Cuts at: 4498 4852 202
Size: 354 2716
Fragments arranged by size:
2716 1237 692 641 555 493 436 354
170 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 2321 2412 2789 3693 4947 5080 5539 5750
Size: 890 91 377 904 1254 133 459 211
Cuts at: 5750 1431
Size: 3047
Fragments arranged by size:
3047 1254 904 890 459 377 211 133
91
TatI w'GTAC_w
Cuts at: 6881 6881
Size: 7366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 733 765 1536 1568 2856 2888 3135
Size: 32 289 32 771 32 1288 32 247
Cuts at: 3135 3167 3278 3310 3811 3843 4249 4281 6883
Size: 32 111 32 501 32 406 32 2602
Cuts at: 6883 6915 412
Size: 32 863
Fragments arranged by size:
2602 1288 863 771 501 406 289 247
111 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2945 3984 5161 2945
Size: 1039 1177 5150
Fragments arranged by size:
5150 1177 1039
XbaI T'CTAG_A
Cuts at: 4395 4395
Size: 7366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1478 3643 4335 4859 141
Size: 1337 2165 692 524 2648
Fragments arranged by size:
2648 2165 1337 692 524
XhoI C'TCGA_G
Cuts at: 3185 3185
Size: 7366
XmaI C'CCGG_G
Cuts at: 1547 2622 3364 4013 4384 1547
Size: 1075 742 649 371 4529
Fragments arranged by size:
4529 1075 742 649 371
XmnI GAAnn'nnTTC
Cuts at: 238 4120 238
Size: 3882 3484
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI BaeI BfrBI BmgBI BmtI
BsiWI BsrGI BssHII BstZ17I CspCI EcoRV FspAI HpaI
MfeI NheI NotI NsiI PacI PshAI PsiI PsrI
SacII SgrAI SspI SwaI ZraI