Restriction map for
pF1KSDA1083
(Circular) (Six-base) MAPSORT of: pF1KSDA1083.seq Check: 6203 from: 1 to: 4891
pF1KSDA1083 4891 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 429 429
Size: 4891
AccI GT'mk_AC
Cuts at: 1404 1927 1404
Size: 523 4368
Acc65I G'GTAC_C
Cuts at: 1905 1905
Size: 4891
AclI AA'CG_TT
Cuts at: 4731 4731
Size: 4891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 3146 3895 4163 2714
Size: 432 749 268 3442
Fragments arranged by size:
3442 749 432 268
AfeI AGC'GCT
Cuts at: 367 367
Size: 4891
AflIII A'CryG_T
Cuts at: 2241 3347 4324 2241
Size: 1106 977 2808
Fragments arranged by size:
2808 1106 977
AgeI A'CCGG_T
Cuts at: 3279 3279
Size: 4891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 4891
AlwNI CAG_nnn'CTG
Cuts at: 421 1216 2194 3763 421
Size: 795 978 1569 1549
Fragments arranged by size:
1569 1549 978 795
ApaLI G'TGCA_C
Cuts at: 3661 3661
Size: 4891
ApoI r'AATT_y
Cuts at: 129 975 1048 1420 1432 1893 4244 4500 129
Size: 846 73 372 12 461 2351 256 520
Fragments arranged by size:
2351 846 520 461 372 256 73 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4891
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4891
AvaI C'yCGr_G
Cuts at: 250 1319 1909 250
Size: 1069 590 3232
Fragments arranged by size:
3232 1069 590
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1859 1892 1859
Size: 33 4858
BamHI G'GATC_C
Cuts at: 1914 1914
Size: 4891
BanI G'GyrC_C
Cuts at: 169 183 441 447 1905 2319 2567 2602 169
Size: 14 258 6 1458 414 248 35 2458
Fragments arranged by size:
2458 1458 414 258 248 35 14 6
BanII G_rGCy'C
Cuts at: 344 403 1149 1305 1903 2933 344
Size: 59 746 156 598 1030 2302
Fragments arranged by size:
2302 1030 746 598 156 59
BbeI G_GCGC'C
Cuts at: 173 445 2323 2571 173
Size: 272 1878 248 2493
Fragments arranged by size:
2493 1878 272 248
BbsI GAAGACnn'nnnn_
Cuts at: 471 1198 471
Size: 727 4164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 407 441 407
Size: 34 4857
BciVI GTATCCnnnnn_n'
Cuts at: 1651 2787 3550 4891 1651
Size: 1136 763 1341 1651
Fragments arranged by size:
1651 1341 1136 763
BclI T'GATC_A
Cuts at: 2409 2409
Size: 4891
BglI GCCn_nnn'nGGC
Cuts at: 192 238 385 812 192
Size: 46 147 427 4271
Fragments arranged by size:
4271 427 147 46
BglII A'GATC_T
Cuts at: 2404 4443 2404
Size: 2039 2852
BlpI GC'TnA_GC
Cuts at: 2001 2001
Size: 4891
Bme1580I G_kGCm'C
Cuts at: 2514 2607 3665 2514
Size: 93 1058 3740
Fragments arranged by size:
3740 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 2101 2182 2517 4663 1711
Size: 390 81 335 2146 1939
Fragments arranged by size:
2146 1939 390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 522 3256 522
Size: 2734 2157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1475 878
Size: 597 4294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 2944 3438 3736 2065
Size: 879 494 298 3220
Fragments arranged by size:
3220 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4595 4595
Size: 4891
BsaAI yAC'GTr
Cuts at: 1275 1881 2242 1275
Size: 606 361 3924
Fragments arranged by size:
3924 606 361
BsaBI GATnn'nnATC
Cuts at: 6 2403 4442 6
Size: 2397 2039 455
Fragments arranged by size:
2397 2039 455
BsaHI Gr'CG_yC
Cuts at: 170 442 651 1407 2320 2568 3272 4768 170
Size: 272 209 756 913 248 704 1496 293
Fragments arranged by size:
1496 913 756 704 293 272 248 209
BsaWI w'CCGG_w
Cuts at: 2078 2599 3279 3553 3700 4111 2078
Size: 521 680 274 147 411 2858
Fragments arranged by size:
2858 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 4861
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 411 1576 334
Size: 77 1165 3649
Fragments arranged by size:
3649 1165 77
BseYI C'CCAG_C
Cuts at: 2314 3072 3651 4147 2314
Size: 758 579 496 3058
Fragments arranged by size:
3058 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2431 2477 3687 104
Size: 2327 46 1210 1308
Fragments arranged by size:
2327 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 403 828 1149 1394 1903 2681 2871 3665 403
Size: 425 321 245 509 778 190 794 1629
Fragments arranged by size:
1629 794 778 509 425 321 245 190
BsmI GAATG_Cn'
Cuts at: 1233 1233
Size: 4891
Bsp1286I G_dGCh'C
Cuts at: 344 403 828 1149 1305 1394 1903 2514 2607
Size: 59 425 321 156 89 509 611 93
Cuts at: 2607 2681 2871 2933 3665 344
Size: 74 190 62 732 1570
Fragments arranged by size:
1570 732 611 509 425 321 190 156
93 89 74 62 59
BspEI T'CCGG_A
Cuts at: 4111 4111
Size: 4891
BspHI T'CATG_A
Cuts at: 4067 4067
Size: 4891
BspMI ACCTGCnnnn'nnnn_
Cuts at: 429 1939 2153 2455 2836 429
Size: 1510 214 302 381 2484
Fragments arranged by size:
2484 1510 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3181 3235 4721 4886 50
Size: 3131 54 1486 165 55
Fragments arranged by size:
3131 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 509 1087 2801 509
Size: 578 1714 2599
Fragments arranged by size:
2599 1714 578
BsrFI r'CCGG_y
Cuts at: 237 444 2887 3068 3279 237
Size: 207 2443 181 211 1849
Fragments arranged by size:
2443 1849 211 207 181
BsrGI T'GTAC_A
Cuts at: 1443 1443
Size: 4891
BssSI C'ACGA_G
Cuts at: 3160 3520 3160
Size: 360 4531
BstAPI GCAn_nnn'nTGC
Cuts at: 1216 1639 1693 2266 1216
Size: 423 54 573 3841
Fragments arranged by size:
3841 573 423 54
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4891
BstYI r'GATC_y
Cuts at: 1 796 871 1914 2381 2404 2739 2985 3999
Size: 795 75 1043 467 23 335 246 1014
Cuts at: 3999 4085 4097 4443 1
Size: 86 12 346 449
Fragments arranged by size:
1043 1014 795 467 449 346 335 246
86 75 23 12
BtgI C'CryG_G
Cuts at: 848 848
Size: 4891
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2839 3020 3183 2839
Size: 181 163 4547
Fragments arranged by size:
4547 181 163
BtsI GCAGTG_nn'
Cuts at: 763 2132 763
Size: 1369 3522
ClaI AT'CG_AT
Cuts at: 524 524
Size: 4891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1256 1291 1256
Size: 35 4856
DraI TTT'AAA
Cuts at: 1888 1888
Size: 4891
DrdI GACnn_nn'nnGTC
Cuts at: 2595 3455 2595
Size: 860 4031
EaeI y'GGCC_r
Cuts at: 686 2474 2648 3039 3066 4413 686
Size: 1788 174 391 27 1347 1164
Fragments arranged by size:
1788 1347 1164 391 174 27
EagI C'GGCC_G
Cuts at: 2474 2474
Size: 4891
EarI CTCTTCn'nnn_
Cuts at: 352 1374 2912 3122 352
Size: 1022 1538 210 2121
Fragments arranged by size:
2121 1538 1022 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 188 236 3097 3409 3555 4701 188
Size: 48 2861 312 146 1146 378
Fragments arranged by size:
2861 1146 378 312 146 48
EcoICRI GAG'CTC
Cuts at: 401 1147 1901 401
Size: 746 754 3391
Fragments arranged by size:
3391 754 746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1475 2714 3146 3895 4163 878
Size: 597 1239 432 749 268 1606
Fragments arranged by size:
1606 1239 749 597 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1154 1154
Size: 4891
EcoO109I rG'GnC_Cy
Cuts at: 1705 2028 1705
Size: 323 4568
EcoRI G'AATT_C
Cuts at: 129 1893 129
Size: 1764 3127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 714 1280 2908 714
Size: 566 1628 2697
Fragments arranged by size:
2697 1628 566
FseI GG_CCGG'CC
Cuts at: 241 241
Size: 4891
FspI TGC'GCA
Cuts at: 520 2670 520
Size: 2150 2741
HaeII r_GCGC'y
Cuts at: 173 369 445 2323 2571 3595 4574 173
Size: 196 76 1878 248 1024 979 490
Fragments arranged by size:
1878 1024 979 490 248 196 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1645 1677 2731 2761 2763 2793 1645
Size: 32 1054 30 2 30 3743
Fragments arranged by size:
3743 1054 32 30 30 2
HincII GTy'rAC
Cuts at: 1928 1928
Size: 4891
KasI G'GCGC_C
Cuts at: 169 441 2319 2567 169
Size: 272 1878 248 2493
Fragments arranged by size:
2493 1878 272 248
KpnI G_GTAC'C
Cuts at: 1909 1909
Size: 4891
MluI A'CGCG_T
Cuts at: 4324 4324
Size: 4891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1828 3562 3746 1828
Size: 1734 184 2973
Fragments arranged by size:
2973 1734 184
MscI TGG'CCA
Cuts at: 688 2650 688
Size: 1962 2929
MslI CAynn'nnrTG
Cuts at: 2878 3005 2878
Size: 127 4764
MspA1I CmG'CkG
Cuts at: 294 1042 2000 2314 2674 3689 3934 294
Size: 748 958 314 360 1015 245 1251
Fragments arranged by size:
1251 1015 958 748 360 314 245
NaeI GCC'GGC
Cuts at: 239 3070 239
Size: 2831 2060
NarI GG'CG_CC
Cuts at: 170 442 2320 2568 170
Size: 272 1878 248 2493
Fragments arranged by size:
2493 1878 272 248
NcoI C'CATG_G
Cuts at: 848 848
Size: 4891
NgoMIV G'CCGG_C
Cuts at: 237 3068 237
Size: 2831 2060
NruI TCG'CGA
Cuts at: 3252 3252
Size: 4891
NspI r_CATG'y
Cuts at: 1942 2442 3351 1942
Size: 500 909 3482
Fragments arranged by size:
3482 909 500
PciI A'CATG_T
Cuts at: 3347 3347
Size: 4891
PmeI GTTT'AAAC
Cuts at: 1888 1888
Size: 4891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4030 4062 4030
Size: 32 4859
PpuMI rG'GwC_Cy
Cuts at: 1705 1705
Size: 4891
PsiI TTA'TAA
Cuts at: 1012 1012
Size: 4891
PstI C_TGCA'G
Cuts at: 804 1221 1936 2621 804
Size: 417 715 685 3074
Fragments arranged by size:
3074 715 685 417
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4891
PvuII CAG'CTG
Cuts at: 1042 2314 2674 1042
Size: 1272 360 3259
Fragments arranged by size:
3259 1272 360
RsrII CG'GwC_CG
Cuts at: 3084 3084
Size: 4891
SacI G_AGCT'C
Cuts at: 403 1149 1903 403
Size: 746 754 3391
Fragments arranged by size:
3391 754 746
SalI G'TCGA_C
Cuts at: 1926 1926
Size: 4891
SapI GCTCTTCn'nnn_
Cuts at: 2912 3122 2912
Size: 210 4681
SbfI CC_TGCA'GG
Cuts at: 1936 1936
Size: 4891
ScaI AGT'ACT
Cuts at: 1469 4408 1469
Size: 2939 1952
SfcI C'TryA_G
Cuts at: 33 800 1217 1932 2617 3612 3803 33
Size: 767 417 715 685 995 191 1121
Fragments arranged by size:
1121 995 767 715 685 417 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 812 812
Size: 4891
SfoI GGC'GCC
Cuts at: 171 443 2321 2569 171
Size: 272 1878 248 2493
Fragments arranged by size:
2493 1878 272 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4891
SgrAI Cr'CCGG_yG
Cuts at: 444 444
Size: 4891
SmaI CCC'GGG
Cuts at: 1911 1911
Size: 4891
SmlI C'TyrA_G
Cuts at: 1319 2044 2959 3453 3715 1319
Size: 725 915 494 262 2495
Fragments arranged by size:
2495 915 725 494 262
SnaBI TAC'GTA
Cuts at: 1881 1881
Size: 4891
SpeI A'CTAG_T
Cuts at: 67 754 67
Size: 687 4204
SphI G_CATG'C
Cuts at: 1942 2442 1942
Size: 500 4391
SspI AAT'ATT
Cuts at: 1767 1767
Size: 4891
StuI AGG'CCT
Cuts at: 195 493 806 1122 195
Size: 298 313 316 3964
Fragments arranged by size:
3964 316 313 298
StyI C'CwwG_G
Cuts at: 689 848 2023 2377 689
Size: 159 1175 354 3203
Fragments arranged by size:
3203 1175 354 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1468 2472 2605 3064 3275 1468
Size: 1004 133 459 211 3084
Fragments arranged by size:
3084 1004 459 211 133
TatI w'GTAC_w
Cuts at: 275 500 1443 1467 4406 275
Size: 225 943 24 2939 760
Fragments arranged by size:
2939 943 760 225 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4408 4440 4408
Size: 32 4859
Tth111I GACn'n_nGTC
Cuts at: 2686 2686
Size: 4891
XbaI T'CTAG_A
Cuts at: 708 1920 708
Size: 1212 3679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2384 2384
Size: 4891
XhoI C'TCGA_G
Cuts at: 1319 1319
Size: 4891
XmaI C'CCGG_G
Cuts at: 1909 1909
Size: 4891
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I DraIII EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SrfI SwaI XmnI ZraI