Restriction map for
pF1KSDA1087
(Circular) (Six-base) MAPSORT of: pF1KSDA1087.seq Check: 5516 from: 1 to: 5900
pF1KSDA1087 5900 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 365 2938 365
Size: 2573 3327
Acc65I G'GTAC_C
Cuts at: 2916 2916
Size: 5900
AclI AA'CG_TT
Cuts at: 5740 5740
Size: 5900
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 3725 4157 4904 5172 1882
Size: 1843 432 747 268 2610
Fragments arranged by size:
2610 1843 747 432 268
AfeI AGC'GCT
Cuts at: 630 901 630
Size: 271 5629
AflII C'TTAA_G
Cuts at: 1975 1975
Size: 5900
AflIII A'CryG_T
Cuts at: 368 641 890 1850 2516 2561 2600 3252 4356
Size: 273 249 960 666 45 39 652 1104
Cuts at: 4356 5333 368
Size: 977 935
Fragments arranged by size:
1104 977 960 935 666 652 273 249
45 39
AgeI A'CCGG_T
Cuts at: 4288 4288
Size: 5900
AhdI GACnn_n'nnGTC
Cuts at: 312 312
Size: 5900
AleI CACnn'nnGTG
Cuts at: 619 982 1477 619
Size: 363 495 5042
Fragments arranged by size:
5042 495 363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2755 2755
Size: 5900
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 964 996 1339 1371 114
Size: 32 818 32 343 32 4643
Fragments arranged by size:
4643 818 343 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3205 4772 3205
Size: 1567 4333
ApaI G_GGCC'C
Cuts at: 610 1024 1030 610
Size: 414 6 5480
Fragments arranged by size:
5480 414 6
ApaLI G'TGCA_C
Cuts at: 1837 4670 1837
Size: 2833 3067
ApoI r'AATT_y
Cuts at: 2904 5253 5509 2904
Size: 2349 256 3295
Fragments arranged by size:
3295 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
AvaI C'yCGr_G
Cuts at: 1023 1770 2264 2920 1023
Size: 747 494 656 4003
Fragments arranged by size:
4003 747 656 494
BamHI G'GATC_C
Cuts at: 2925 2925
Size: 5900
BanI G'GyrC_C
Cuts at: 481 613 937 988 1213 1263 1300 1647 1741
Size: 132 324 51 225 50 37 347 94
Cuts at: 1741 1783 2500 2753 2916 3330 3578 3613 481
Size: 42 717 253 163 414 248 35 2768
Fragments arranged by size:
2768 717 414 347 324 253 248 225
163 132 94 51 50 42 37 35
BanII G_rGCy'C
Cuts at: 305 538 610 1024 1030 1450 2914 3944 305
Size: 233 72 414 6 420 1464 1030 2261
Fragments arranged by size:
2261 1464 1030 420 414 233 72 6
BbeI G_GCGC'C
Cuts at: 941 992 1217 1267 1304 1651 2757 3334 3582
Size: 51 225 50 37 347 1106 577 248
Cuts at: 3582 941
Size: 3259
Fragments arranged by size:
3259 1106 577 347 248 225 51 50
37
BbsI GAAGACnn'nnnn_
Cuts at: 708 819 1298 2478 2709 708
Size: 111 479 1180 231 3899
Fragments arranged by size:
3899 1180 479 231 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1299 1333 1299
Size: 34 5866
BciVI GTATCCnnnnn_n'
Cuts at: 3798 4559 5900 3798
Size: 761 1341 3798
Fragments arranged by size:
3798 1341 761
BclI T'GATC_A
Cuts at: 3420 3420
Size: 5900
BglI GCCn_nnn'nGGC
Cuts at: 859 1385 1635 1971 859
Size: 526 250 336 4788
Fragments arranged by size:
4788 526 336 250
BglII A'GATC_T
Cuts at: 3415 5452 3415
Size: 2037 3863
BlpI GC'TnA_GC
Cuts at: 3012 3012
Size: 5900
Bme1580I G_kGCm'C
Cuts at: 484 610 1024 1030 1474 1744 1841 2348 2503
Size: 126 414 6 444 270 97 507 155
Cuts at: 2503 3525 3618 4674 484
Size: 1022 93 1056 1710
Fragments arranged by size:
1710 1056 1022 507 444 414 270 155
126 97 93 6
BmgBI CAC'GTC
Cuts at: 428 1752 428
Size: 1324 4576
BmrI ACTGGGnnnn_n'
Cuts at: 1962 2655 3112 3193 3528 5672 1962
Size: 693 457 81 335 2144 2190
Fragments arranged by size:
2190 2144 693 457 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1449 4267 1449
Size: 2818 3082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1363 1885 2122 2881 1138
Size: 225 522 237 759 4157
Fragments arranged by size:
4157 759 522 237 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1075 2446 3076 3955 4447 4745 1066
Size: 9 1371 630 879 492 298 2221
Fragments arranged by size:
2221 1371 879 630 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 463 1876 5604 463
Size: 1413 3728 759
Fragments arranged by size:
3728 1413 759
BsaAI yAC'GTr
Cuts at: 891 1376 1410 1725 2298 2892 3253 891
Size: 485 34 315 573 594 361 3538
Fragments arranged by size:
3538 594 573 485 361 315 34
BsaBI GATnn'nnATC
Cuts at: 6 448 3414 5451 6
Size: 442 2966 2037 455
Fragments arranged by size:
2966 2037 455 442
BsaHI Gr'CG_yC
Cuts at: 938 989 1052 1214 1247 1264 1301 1648 2618
Size: 51 63 162 33 17 37 347 970
Cuts at: 2618 2754 3331 3579 4281 5777 938
Size: 136 577 248 702 1496 1061
Fragments arranged by size:
1496 1061 970 702 577 347 248 162
136 63 51 37 33 17
BsaWI w'CCGG_w
Cuts at: 3089 3610 4288 4562 4709 5120 3089
Size: 521 678 274 147 411 3869
Fragments arranged by size:
3869 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 114 144 1339 1369 2245 2275 2419 2449 114
Size: 30 1195 30 876 30 144 30 3565
Fragments arranged by size:
3565 1195 876 144 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 528 1558 2053 2056 2059 2131 2257 2260
Size: 380 1030 495 3 3 72 126 3
Cuts at: 2260 2263 2266 2284 148
Size: 3 3 18 3764
Fragments arranged by size:
3764 1030 495 380 126 72 18 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 318 603 678 1008 1956 2369 2778 3325 4083
Size: 285 75 330 948 413 409 547 758
Cuts at: 4083 4660 5156 318
Size: 577 496 1062
Fragments arranged by size:
1062 948 758 577 547 496 413 409
330 285 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1705 2433 2674 1141
Size: 564 728 241 4367
Fragments arranged by size:
4367 728 564 241
BsiEI CG_ry'CG
Cuts at: 104 399 1271 2761 3442 3488 4696 104
Size: 295 872 1490 681 46 1208 1308
Fragments arranged by size:
1490 1308 1208 872 681 295 46
BsiHKAI G_wGCw'C
Cuts at: 1841 2333 2914 3692 3882 4674 1841
Size: 492 581 778 190 792 3067
Fragments arranged by size:
3067 792 778 581 492 190
BsmBI CGTCTCn'nnnn_
Cuts at: 496 1489 496
Size: 993 4907
Bsp1286I G_dGCh'C
Cuts at: 305 484 538 610 1024 1030 1450 1474 1744
Size: 179 54 72 414 6 420 24 270
Cuts at: 1744 1841 2333 2348 2503 2914 3525 3618 3692
Size: 97 492 15 155 411 611 93 74
Cuts at: 3692 3882 3944 4674 305
Size: 190 62 730 1531
Fragments arranged by size:
1531 730 611 492 420 414 411 270
190 179 155 97 93 74 72 62
54 24 15 6
BspEI T'CCGG_A
Cuts at: 5120 5120
Size: 5900
BspHI T'CATG_A
Cuts at: 5076 5076
Size: 5900
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1586 2950 3164 3466 3847 1586
Size: 1364 214 302 381 3639
Fragments arranged by size:
3639 1364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2274 4192 4246 5730 5895 50
Size: 2224 1918 54 1484 165 55
Fragments arranged by size:
2224 1918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2732 3812 2732
Size: 1080 4820
BsrFI r'CCGG_y
Cuts at: 682 1210 1266 2096 2581 2750 2756 3898 4079
Size: 528 56 830 485 169 6 1142 181
Cuts at: 4079 4288 682
Size: 209 2294
Fragments arranged by size:
2294 1142 830 528 485 209 181 169
56 6
BsrGI T'GTAC_A
Cuts at: 893 2840 893
Size: 1947 3953
BssHII G'CGCG_C
Cuts at: 1039 1775 1777 1822 1039
Size: 736 2 45 5117
Fragments arranged by size:
5117 736 45 2
BssSI C'ACGA_G
Cuts at: 4171 4529 4171
Size: 358 5542
BstAPI GCAn_nnn'nTGC
Cuts at: 3277 3277
Size: 5900
BstBI TT'CG_AA
Cuts at: 110 4261 110
Size: 4151 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2532 2532
Size: 5900
BstYI r'GATC_y
Cuts at: 1 551 1076 1113 2925 3392 3415 3750 3996
Size: 550 525 37 1812 467 23 335 246
Cuts at: 3996 5008 5094 5106 5452 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1812 1012 550 525 467 449 346 335
246 86 37 23 12
BtgI C'CryG_G
Cuts at: 125 359 1745 2364 125
Size: 234 1386 619 3661
Fragments arranged by size:
3661 1386 619 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 375 639 1119 3850 4031 4194 375
Size: 264 480 2731 181 163 2081
Fragments arranged by size:
2731 2081 480 264 181 163
BtsI GCAGTG_nn'
Cuts at: 347 725 1335 3143 347
Size: 378 610 1808 3104
Fragments arranged by size:
3104 1808 610 378
DraI TTT'AAA
Cuts at: 2899 2899
Size: 5900
DrdI GACnn_nn'nnGTC
Cuts at: 1756 3606 4464 1756
Size: 1850 858 3192
Fragments arranged by size:
3192 1850 858
EaeI y'GGCC_r
Cuts at: 221 356 410 579 860 1268 1658 2633 2758
Size: 135 54 169 281 408 390 975 125
Cuts at: 2758 3485 3659 4050 4077 5422 221
Size: 727 174 391 27 1345 699
Fragments arranged by size:
1345 975 727 699 408 391 390 281
174 169 135 125 54 27
EagI C'GGCC_G
Cuts at: 1268 2758 3485 1268
Size: 1490 727 3683
Fragments arranged by size:
3683 1490 727
EarI CTCTTCn'nnn_
Cuts at: 883 952 1162 1901 2338 2713 2824 2854 3923
Size: 69 210 739 437 375 111 30 1069
Cuts at: 3923 4133 883
Size: 210 2650
Fragments arranged by size:
2650 1069 739 437 375 210 210 111
69 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1258 4108 4418 4564 5710 1258
Size: 2850 310 146 1146 1448
Fragments arranged by size:
2850 1448 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2912 2912
Size: 5900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1363 1882 1885 2122 2881 3725 4157 4904
Size: 225 519 3 237 759 844 432 747
Cuts at: 4904 5172 1138
Size: 268 1866
Fragments arranged by size:
1866 844 759 747 519 432 268 237
225 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1254 1254
Size: 5900
EcoO109I rG'GnC_Cy
Cuts at: 260 996 1027 1086 2186 2261 2427 3039 260
Size: 736 31 59 1100 75 166 612 3121
Fragments arranged by size:
3121 1100 736 612 166 75 59 31
EcoRI G'AATT_C
Cuts at: 2904 2904
Size: 5900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2349 3919 2349
Size: 1570 4330
FspI TGC'GCA
Cuts at: 1231 1513 1765 2677 3681 1231
Size: 282 252 912 1004 3450
Fragments arranged by size:
3450 1004 912 282 252
FspAI rTGC'GCAy
Cuts at: 2677 2677
Size: 5900
HaeII r_GCGC'y
Cuts at: 632 814 903 941 992 1217 1267 1304 1651
Size: 182 89 38 51 225 50 37 347
Cuts at: 1651 2542 2757 3334 3582 4604 5583 632
Size: 891 215 577 248 1022 979 949
Fragments arranged by size:
1022 979 949 891 577 347 248 225
215 182 89 51 50 38 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 371 403 493 525 1977 2009 2245
Size: 32 41 32 90 32 1452 32 236
Cuts at: 2245 2277 2419 2451 2786 2818 3742 3772 3774
Size: 32 142 32 335 32 924 30 2
Cuts at: 3774 3804 298
Size: 30 2394
Fragments arranged by size:
2394 1452 924 335 236 142 90 41
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2939 2939
Size: 5900
KasI G'GCGC_C
Cuts at: 937 988 1213 1263 1300 1647 2753 3330 3578
Size: 51 225 50 37 347 1106 577 248
Cuts at: 3578 937
Size: 3259
Fragments arranged by size:
3259 1106 577 347 248 225 51 50
37
KpnI G_GTAC'C
Cuts at: 2920 2920
Size: 5900
MluI A'CGCG_T
Cuts at: 1850 2561 5333 1850
Size: 711 2772 2417
Fragments arranged by size:
2772 2417 711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 1475 2612 4571 4755 536
Size: 939 1137 1959 184 1681
Fragments arranged by size:
1959 1681 1137 939 184
MscI TGG'CCA
Cuts at: 358 1660 3661 358
Size: 1302 2001 2597
Fragments arranged by size:
2597 2001 1302
MslI CAynn'nnrTG
Cuts at: 523 619 646 658 982 1477 2737 3889 4016
Size: 96 27 12 324 495 1260 1152 127
Cuts at: 4016 523
Size: 2407
Fragments arranged by size:
2407 1260 1152 495 324 127 96 27
12
MspA1I CmG'CkG
Cuts at: 269 923 1125 1653 3011 3325 3685 4698 4943
Size: 654 202 528 1358 314 360 1013 245
Cuts at: 4943 269
Size: 1226
Fragments arranged by size:
1358 1226 1013 654 528 360 314 245
202
NaeI GCC'GGC
Cuts at: 684 1268 2098 2758 4081 684
Size: 584 830 660 1323 2503
Fragments arranged by size:
2503 1323 830 660 584
NarI GG'CG_CC
Cuts at: 938 989 1214 1264 1301 1648 2754 3331 3579
Size: 51 225 50 37 347 1106 577 248
Cuts at: 3579 938
Size: 3259
Fragments arranged by size:
3259 1106 577 347 248 225 51 50
37
NcoI C'CATG_G
Cuts at: 125 359 125
Size: 234 5666
NdeI CA'TA_TG
Cuts at: 2124 2124
Size: 5900
NgoMIV G'CCGG_C
Cuts at: 682 1266 2096 2756 4079 682
Size: 584 830 660 1323 2503
Fragments arranged by size:
2503 1323 830 660 584
NspI r_CATG'y
Cuts at: 372 645 1605 2953 3453 4360 372
Size: 273 960 1348 500 907 1912
Fragments arranged by size:
1912 1348 960 907 500 273
PasI CC'CwG_GG
Cuts at: 531 2496 531
Size: 1965 3935
PciI A'CATG_T
Cuts at: 368 641 4356 368
Size: 273 3715 1912
Fragments arranged by size:
3715 1912 273
PflMI CCAn_nnn'nTGG
Cuts at: 1341 2083 1341
Size: 742 5158
PfoI T'CCnGG_A
Cuts at: 1673 1706 1673
Size: 33 5867
PmeI GTTT'AAAC
Cuts at: 2899 2899
Size: 5900
PmlI CAC'GTG
Cuts at: 1376 1725 1376
Size: 349 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 1339 1371 5039 5071 114
Size: 32 1193 32 3668 32 943
Fragments arranged by size:
3668 1193 943 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 260 1086 2186 2261 2427 260
Size: 826 1100 75 166 3733
Fragments arranged by size:
3733 1100 826 166 75
PshAI GACnn'nnGTC
Cuts at: 823 823
Size: 5900
PspOMI G'GGCC_C
Cuts at: 606 1020 1026 606
Size: 414 6 5480
Fragments arranged by size:
5480 414 6
PstI C_TGCA'G
Cuts at: 2546 2947 3632 2546
Size: 401 685 4814
Fragments arranged by size:
4814 685 401
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5900
PvuII CAG'CTG
Cuts at: 1125 3325 3685 1125
Size: 2200 360 3340
Fragments arranged by size:
3340 2200 360
RsrII CG'GwC_CG
Cuts at: 4095 4095
Size: 5900
SacI G_AGCT'C
Cuts at: 2914 2914
Size: 5900
SalI G'TCGA_C
Cuts at: 2937 2937
Size: 5900
SanDI GG'GwC_CC
Cuts at: 2186 2261 2186
Size: 75 5825
SapI GCTCTTCn'nnn_
Cuts at: 883 952 1162 2338 2713 2824 3923 4133 883
Size: 69 210 1176 375 111 1099 210 2650
Fragments arranged by size:
2650 1176 1099 375 210 210 111 69
SbfI CC_TGCA'GG
Cuts at: 2947 2947
Size: 5900
ScaI AGT'ACT
Cuts at: 2359 5417 2359
Size: 3058 2842
SfcI C'TryA_G
Cuts at: 33 2542 2943 3628 4621 4812 33
Size: 2509 401 685 993 191 1121
Fragments arranged by size:
2509 1121 993 685 401 191
SfoI GGC'GCC
Cuts at: 939 990 1215 1265 1302 1649 2755 3332 3580
Size: 51 225 50 37 347 1106 577 248
Cuts at: 3580 939
Size: 3259
Fragments arranged by size:
3259 1106 577 347 248 225 51 50
37
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
SmaI CCC'GGG
Cuts at: 1025 2266 2922 1025
Size: 1241 656 4003
Fragments arranged by size:
4003 1241 656
SmlI C'TyrA_G
Cuts at: 1081 1090 1975 2461 3055 3970 4462 4724 1081
Size: 9 885 486 594 915 492 262 2257
Fragments arranged by size:
2257 915 885 594 492 486 262 9
SnaBI TAC'GTA
Cuts at: 2892 2892
Size: 5900
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5900
SphI G_CATG'C
Cuts at: 2953 3453 2953
Size: 500 5400
SrfI GCCC'GGGC
Cuts at: 1025 1025
Size: 5900
StyI C'CwwG_G
Cuts at: 125 140 359 455 1439 1631 2174 2607 3034
Size: 15 219 96 984 192 543 433 427
Cuts at: 3034 3388 125
Size: 354 2637
Fragments arranged by size:
2637 984 543 433 427 354 219 192
96 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 3483 3616 4075 4284 310
Size: 3173 133 459 209 1926
Fragments arranged by size:
3173 1926 459 209 133
TatI w'GTAC_w
Cuts at: 347 893 1460 2357 2642 2840 5415 347
Size: 546 567 897 285 198 2575 832
Fragments arranged by size:
2575 897 832 567 546 285 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 536 568 5417 5449 536
Size: 32 4849 32 987
Fragments arranged by size:
4849 987 32 32
Tth111I GACn'n_nGTC
Cuts at: 507 2470 3697 507
Size: 1963 1227 2710
Fragments arranged by size:
2710 1963 1227
XbaI T'CTAG_A
Cuts at: 2931 2931
Size: 5900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1638 3395 132
Size: 1506 1757 2637
Fragments arranged by size:
2637 1757 1506
XmaI C'CCGG_G
Cuts at: 1023 2264 2920 1023
Size: 1241 656 4003
Fragments arranged by size:
4003 1241 656
XmnI GAAnn'nnTTC
Cuts at: 2602 2602
Size: 5900
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AscI AvrII BaeI BbvCI BfrBI BmtI
Bpu10I BsiWI BsmI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SspI StuI SwaI XhoI ZraI