Restriction map for
pF1KSDA1088
(Circular) (Six-base) MAPSORT of: pF1KSDA1088.seq Check: 7114 from: 1 to: 7337
pF1KSDA1088 7337 bp
With 182 enzymes: SgfI *
February 1, 2012 16:00 ..
AatII G_ACGT'C
Cuts at: 959 959
Size: 7337
AccI GT'mk_AC
Cuts at: 158 4375 158
Size: 4217 3120
Acc65I G'GTAC_C
Cuts at: 2171 4353 2171
Size: 2182 5155
AclI AA'CG_TT
Cuts at: 7177 7177
Size: 7337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 871 929 1099 1205 1482 1900 2542 3928
Size: 81 58 170 106 277 418 642 1386
Cuts at: 3928 5162 5594 6341 6609 790
Size: 1234 432 747 268 1518
Fragments arranged by size:
1518 1386 1234 747 642 432 418 277
268 170 106 81 58
AfeI AGC'GCT
Cuts at: 3371 3505 3916 4292 3371
Size: 134 411 376 6416
Fragments arranged by size:
6416 411 376 134
AflIII A'CryG_T
Cuts at: 4238 4689 5793 6770 4238
Size: 451 1104 977 4805
Fragments arranged by size:
4805 1104 977 451
AgeI A'CCGG_T
Cuts at: 5725 5725
Size: 7337
AhdI GACnn_n'nnGTC
Cuts at: 955 955
Size: 7337
AleI CACnn'nnGTG
Cuts at: 3516 4202 3516
Size: 686 6651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3499 4220 3499
Size: 721 6616
AlwNI CAG_nnn'CTG
Cuts at: 684 972 1251 1299 2112 2602 2880 3171 3700
Size: 288 279 48 813 490 278 291 529
Cuts at: 3700 3769 4642 6209 684
Size: 69 873 1567 1812
Fragments arranged by size:
1812 1567 873 813 529 490 291 288
279 278 69 48
ApaI G_GGCC'C
Cuts at: 1851 3336 3836 1851
Size: 1485 500 5352
Fragments arranged by size:
5352 1485 500
ApaLI G'TGCA_C
Cuts at: 285 1393 2795 3519 6107 285
Size: 1108 1402 724 2588 1515
Fragments arranged by size:
2588 1515 1402 1108 724
ApoI r'AATT_y
Cuts at: 4341 6690 6946 4341
Size: 2349 256 4732
Fragments arranged by size:
4732 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7337
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7337
AvaI C'yCGr_G
Cuts at: 545 2087 2408 2560 3546 4357 545
Size: 1542 321 152 986 811 3525
Fragments arranged by size:
3525 1542 986 811 321 152
BamHI G'GATC_C
Cuts at: 3132 4362 3132
Size: 1230 6107
BanI G'GyrC_C
Cuts at: 1779 1972 2171 2225 3126 3882 3938 4102 4196
Size: 193 199 54 901 756 56 164 94
Cuts at: 4196 4353 4767 5015 5050 1779
Size: 157 414 248 35 4066
Fragments arranged by size:
4066 901 756 414 248 199 193 164
157 94 56 54 35
BanII G_rGCy'C
Cuts at: 253 544 1010 1695 1705 1793 1851 1901 2292
Size: 291 466 685 10 88 58 50 391
Cuts at: 2292 2668 2786 3336 3615 3825 3836 4022 4351
Size: 376 118 550 279 210 11 186 329
Cuts at: 4351 5381 253
Size: 1030 2209
Fragments arranged by size:
2209 1030 685 550 466 391 376 329
291 279 210 186 118 88 58 50
11 10
BbeI G_GCGC'C
Cuts at: 1976 3130 4771 5019 1976
Size: 1154 1641 248 4294
Fragments arranged by size:
4294 1641 1154 248
BbsI GAAGACnn'nnnn_
Cuts at: 274 1070 2241 3649 3658 3999 274
Size: 796 1171 1408 9 341 3612
Fragments arranged by size:
3612 1408 1171 796 341 9
BbvCI CC'TCA_GC
Cuts at: 2945 2945
Size: 7337
BciVI GTATCCnnnnn_n'
Cuts at: 3040 5235 5996 7337 3040
Size: 2195 761 1341 3040
Fragments arranged by size:
3040 2195 1341 761
BclI T'GATC_A
Cuts at: 4857 4857
Size: 7337
BglI GCCn_nnn'nGGC
Cuts at: 349 1788 2410 2622 2917 3316 3331 4194 349
Size: 1439 622 212 295 399 15 863 3492
Fragments arranged by size:
3492 1439 863 622 399 295 212 15
BglII A'GATC_T
Cuts at: 1655 3665 4852 6889 1655
Size: 2010 1187 2037 2103
Fragments arranged by size:
2103 2037 2010 1187
BlpI GC'TnA_GC
Cuts at: 1430 1483 4449 1430
Size: 53 2966 4318
Fragments arranged by size:
4318 2966 53
Bme1580I G_kGCm'C
Cuts at: 289 748 1397 1784 1851 2799 3096 3275 3336
Size: 459 649 387 67 948 297 179 61
Cuts at: 3336 3523 3836 3887 3898 3964 4190 4962 5055
Size: 187 313 51 11 66 226 772 93
Cuts at: 5055 6111 289
Size: 1056 1515
Fragments arranged by size:
1515 1056 948 772 649 459 387 313
297 226 187 179 93 67 66 61
51 11
BmgBI CAC'GTC
Cuts at: 1261 2319 2358 3518 3879 4239 1261
Size: 1058 39 1160 361 360 4359
Fragments arranged by size:
4359 1160 1058 361 360 39
BmrI ACTGGGnnnn_n'
Cuts at: 1609 1689 2358 2417 4177 4549 4630 4965 7109
Size: 80 669 59 1760 372 81 335 2144
Cuts at: 7109 1609
Size: 1837
Fragments arranged by size:
2144 1837 1760 669 372 335 81 80
59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 328 1445 5704 328
Size: 1117 4259 1961
Fragments arranged by size:
4259 1961 1117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 434 447 694 994 1120 1151 1286 1467
Size: 169 13 247 300 126 31 135 181
Cuts at: 1467 1615 1645 1855 2659 3287 3966 4064 4307
Size: 148 30 210 804 628 679 98 243
Cuts at: 4307 265
Size: 3295
Fragments arranged by size:
3295 804 679 628 300 247 243 210
181 169 148 135 126 98 31 30
13
Bpu10I CC'TnA_GC
Cuts at: 2945 3065 2945
Size: 120 7217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2083 4513 5392 5884 6182 2083
Size: 2430 879 492 298 3238
Fragments arranged by size:
3238 2430 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 410 1918 3230 7041 410
Size: 1508 1312 3811 706
Fragments arranged by size:
3811 1508 1312 706
BsaAI yAC'GTr
Cuts at: 4329 4690 4329
Size: 361 6976
BsaBI GATnn'nnATC
Cuts at: 6 1603 4851 6888 6
Size: 1597 3248 2037 455
Fragments arranged by size:
3248 2037 1597 455
BsaHI Gr'CG_yC
Cuts at: 956 1538 1973 2185 3127 4768 5016 5718 7214
Size: 582 435 212 942 1641 248 702 1496
Cuts at: 7214 956
Size: 1079
Fragments arranged by size:
1641 1496 1079 942 702 582 435 248
212
BsaWI w'CCGG_w
Cuts at: 1418 4526 5047 5725 5999 6146 6557 1418
Size: 3108 521 678 274 147 411 2198
Fragments arranged by size:
3108 2198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 883 913 2677 2707 3807 3837 3964
Size: 30 312 30 1764 30 1100 30 127
Cuts at: 3964 3994 4313 4343 541
Size: 30 319 30 3535
Fragments arranged by size:
3535 1764 1100 319 312 127 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 991 1744 2689 2756 2794 3034 3589 991
Size: 753 945 67 38 240 555 4739
Fragments arranged by size:
4739 945 753 555 240 67 38
BseYI C'CCAG_C
Cuts at: 537 1036 1359 1368 2140 2515 3164 3730 3802
Size: 499 323 9 772 375 649 566 72
Cuts at: 3802 3963 4762 5520 6097 6593 537
Size: 161 799 758 577 496 1281
Fragments arranged by size:
1281 799 772 758 649 577 566 499
496 375 323 161 72 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 4226 3448
Size: 778 6559
BsiEI CG_ry'CG
Cuts at: 104 1286 3537 4214 4879 4925 6133 104
Size: 1182 2251 677 665 46 1208 1308
Fragments arranged by size:
2251 1308 1208 1182 677 665 46
BsiHKAI G_wGCw'C
Cuts at: 289 837 1397 1453 1705 2645 2799 3071 3188
Size: 548 560 56 252 940 154 272 117
Cuts at: 3188 3523 3825 4351 5129 5319 6111 289
Size: 335 302 526 778 190 792 1515
Fragments arranged by size:
1515 940 792 778 560 548 526 335
302 272 252 190 154 117 56
BsiWI C'GTAC_G
Cuts at: 4325 4325
Size: 7337
BsmI GAATG_Cn'
Cuts at: 3074 3074
Size: 7337
BsmBI CGTCTCn'nnnn_
Cuts at: 311 1127 2348 311
Size: 816 1221 5300
Fragments arranged by size:
5300 1221 816
Bsp1286I G_dGCh'C
Cuts at: 253 289 544 748 837 1010 1397 1453 1695
Size: 36 255 204 89 173 387 56 242
Cuts at: 1695 1705 1784 1793 1851 1901 2292 2645 2668
Size: 10 79 9 58 50 391 353 23
Cuts at: 2668 2786 2799 3071 3096 3188 3275 3336 3523
Size: 118 13 272 25 92 87 61 187
Cuts at: 3523 3615 3825 3836 3887 3898 3964 4022 4190
Size: 92 210 11 51 11 66 58 168
Cuts at: 4190 4351 4962 5055 5129 5319 5381 6111 253
Size: 161 611 93 74 190 62 730 1479
Fragments arranged by size:
1479 730 611 391 387 353 272 255
242 210 204 190 187 173 168 161
118 93 92 92 89 87 79 74
66 62 61 58 58 56 51 50
36 25 23 13 11 11 10 9
BspEI T'CCGG_A
Cuts at: 6557 6557
Size: 7337
BspHI T'CATG_A
Cuts at: 6513 6513
Size: 7337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2513 2975 3715 3993 4387 4601 4903 5284 2513
Size: 462 740 278 394 214 302 381 4566
Fragments arranged by size:
4566 740 462 394 381 302 278 214
BsrBI CCG'CTC
Cuts at: 50 725 1544 4168 5629 5683 7167 7332 50
Size: 675 819 2624 1461 54 1484 165 55
Fragments arranged by size:
2624 1484 1461 819 675 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1067 5249 1067
Size: 4182 3155
BsrFI r'CCGG_y
Cuts at: 518 1969 2174 2400 2724 3099 3532 3536 4099
Size: 1451 205 226 324 375 433 4 563
Cuts at: 4099 4105 5335 5516 5725 518
Size: 6 1230 181 209 2130
Fragments arranged by size:
2130 1451 1230 563 433 375 324 226
209 205 181 6 4
BssSI C'ACGA_G
Cuts at: 1519 5608 5966 1519
Size: 4089 358 2890
Fragments arranged by size:
4089 2890 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1951 2967 4714 1261
Size: 690 1016 1747 3884
Fragments arranged by size:
3884 1747 1016 690
BstBI TT'CG_AA
Cuts at: 110 5698 110
Size: 5588 1749
BstEII G'GTnAC_C
Cuts at: 1414 2540 1414
Size: 1126 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 210 1869 1983 3865 210
Size: 1659 114 1882 3682
Fragments arranged by size:
3682 1882 1659 114
BstYI r'GATC_y
Cuts at: 1 602 1604 1655 2756 3132 3665 4362 4829
Size: 601 1002 51 1101 376 533 697 467
Cuts at: 4829 4852 5187 5433 6445 6531 6543 6889 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1101 1012 1002 697 601 533 467 449
376 346 335 246 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 524 569 2535 3741 524
Size: 45 1966 1206 4120
Fragments arranged by size:
4120 1966 1206 45
BtgI C'CryG_G
Cuts at: 2040 2786 3152 3344 3753 3815 2040
Size: 746 366 192 409 62 5562
Fragments arranged by size:
5562 746 409 366 192 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1752 3783 5287 5468 5631 1752
Size: 2031 1504 181 163 3458
Fragments arranged by size:
3458 2031 1504 181 163
BtsI GCAGTG_nn'
Cuts at: 1454 3434 4580 1454
Size: 1980 1146 4211
Fragments arranged by size:
4211 1980 1146
ClaI AT'CG_AT
Cuts at: 1112 1112
Size: 7337
DraI TTT'AAA
Cuts at: 4336 4336
Size: 7337
DraIII CAC_nnn'GTG
Cuts at: 741 888 2425 3303 4239 741
Size: 147 1537 878 936 3839
Fragments arranged by size:
3839 1537 936 878 147
DrdI GACnn_nn'nnGTC
Cuts at: 1144 2509 5043 5901 1144
Size: 1365 2534 858 2580
Fragments arranged by size:
2580 2534 1365 858
EaeI y'GGCC_r
Cuts at: 686 1283 1506 1859 2037 2340 2630 2828 2918
Size: 597 223 353 178 303 290 198 90
Cuts at: 2918 3422 3534 4012 4211 4922 5096 5487 5514
Size: 504 112 478 199 711 174 391 27
Cuts at: 5514 6859 686
Size: 1345 1164
Fragments arranged by size:
1345 1164 711 597 504 478 391 353
303 290 223 199 198 178 174 112
90 27
EagI C'GGCC_G
Cuts at: 1283 3534 4211 4922 1283
Size: 2251 677 711 3698
Fragments arranged by size:
3698 2251 711 677
EarI CTCTTCn'nnn_
Cuts at: 1822 2713 2824 5360 5570 1822
Size: 891 111 2536 210 3589
Fragments arranged by size:
3589 2536 891 210 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1318 1519 5545 5855 6001 7147 1318
Size: 201 4026 310 146 1146 1508
Fragments arranged by size:
4026 1508 1146 310 201 146
EcoICRI GAG'CTC
Cuts at: 1703 3823 4349 1703
Size: 2120 526 4691
Fragments arranged by size:
4691 2120 526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 434 447 694 790 871 929 994 1099
Size: 169 13 247 96 81 58 65 105
Cuts at: 1099 1120 1151 1205 1286 1467 1482 1615 1645
Size: 21 31 54 81 181 15 133 30
Cuts at: 1645 1855 1900 2542 2659 3287 3928 3966 4064
Size: 210 45 642 117 628 641 38 98
Cuts at: 4064 4307 5162 5594 6341 6609 265
Size: 243 855 432 747 268 993
Fragments arranged by size:
993 855 747 642 641 628 432 268
247 243 210 181 169 133 117 105
98 96 81 81 65 58 54 45
38 31 30 21 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 676 1750 3193 3403 676
Size: 1074 1443 210 4610
Fragments arranged by size:
4610 1443 1074 210
EcoO109I rG'GnC_Cy
Cuts at: 207 467 1373 1681 2531 2556 2877 2949 3203
Size: 260 906 308 850 25 321 72 254
Cuts at: 3203 3332 3333 3565 3625 3727 3745 3833 4476
Size: 129 1 232 60 102 18 88 643
Cuts at: 4476 207
Size: 3068
Fragments arranged by size:
3068 906 850 643 321 308 260 254
232 129 102 88 72 60 25 18
1
EcoRI G'AATT_C
Cuts at: 4341 4341
Size: 7337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 379 3522 5356 379
Size: 3143 1834 2360
Fragments arranged by size:
3143 2360 1834
FseI GG_CCGG'CC
Cuts at: 3540 3540
Size: 7337
FspI TGC'GCA
Cuts at: 5118 5118
Size: 7337
HaeII r_GCGC'y
Cuts at: 1879 1976 3130 3311 3373 3507 3918 4043 4085
Size: 97 1154 181 62 134 411 125 42
Cuts at: 4085 4294 4771 5019 6041 7020 1879
Size: 209 477 248 1022 979 2196
Fragments arranged by size:
2196 1154 1022 979 477 411 248 209
181 134 125 97 62 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 308 310 340 342 579 611 941 973 1723
Size: 2 30 2 237 32 330 32 750
Cuts at: 1723 1755 2443 2475 5179 5209 5211 5241 308
Size: 32 688 32 2704 30 2 30 2404
Fragments arranged by size:
2704 2404 750 688 330 237 32 32
32 32 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 4376 4376
Size: 7337
KasI G'GCGC_C
Cuts at: 1972 3126 4767 5015 1972
Size: 1154 1641 248 4294
Fragments arranged by size:
4294 1641 1154 248
KpnI G_GTAC'C
Cuts at: 2175 4357 2175
Size: 2182 5155
MluI A'CGCG_T
Cuts at: 6770 6770
Size: 7337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 6008 6192 794
Size: 5214 184 1939
Fragments arranged by size:
5214 1939 184
MscI TGG'CCA
Cuts at: 688 1861 2342 4014 5098 688
Size: 1173 481 1672 1084 2927
Fragments arranged by size:
2927 1672 1173 1084 481
MslI CAynn'nnrTG
Cuts at: 793 1406 3481 3516 3771 4202 5326 5453 793
Size: 613 2075 35 255 431 1124 127 2677
Fragments arranged by size:
2677 2075 1124 613 431 255 127 35
MspA1I CmG'CkG
Cuts at: 555 1142 1434 1562 1887 2548 2655 2690 3036
Size: 587 292 128 325 661 107 35 346
Cuts at: 3036 3090 3171 3346 4210 4448 4762 5122 6135
Size: 54 81 175 864 238 314 360 1013
Cuts at: 6135 6380 555
Size: 245 1512
Fragments arranged by size:
1512 1013 864 661 587 360 346 325
314 292 245 238 175 128 107 81
54 35
NaeI GCC'GGC
Cuts at: 2726 3538 4107 5518 2726
Size: 812 569 1411 4545
Fragments arranged by size:
4545 1411 812 569
NarI GG'CG_CC
Cuts at: 1973 3127 4768 5016 1973
Size: 1154 1641 248 4294
Fragments arranged by size:
4294 1641 1154 248
NgoMIV G'CCGG_C
Cuts at: 2724 3536 4105 5516 2724
Size: 812 569 1411 4545
Fragments arranged by size:
4545 1411 812 569
NspI r_CATG'y
Cuts at: 1518 2512 3480 4390 4890 5797 1518
Size: 994 968 910 500 907 3058
Fragments arranged by size:
3058 994 968 910 907 500
PasI CC'CwG_GG
Cuts at: 1042 1746 1960 2618 3336 1042
Size: 704 214 658 718 5043
Fragments arranged by size:
5043 718 704 658 214
PciI A'CATG_T
Cuts at: 5793 5793
Size: 7337
PflMI CCAn_nnn'nTGG
Cuts at: 939 972 1251 3171 3363 939
Size: 33 279 1920 192 4913
Fragments arranged by size:
4913 1920 279 192 33
PfoI T'CCnGG_A
Cuts at: 209 242 368 463 698 760 1220 2111 3104
Size: 33 126 95 235 62 460 891 993
Cuts at: 3104 3982 209
Size: 878 3564
Fragments arranged by size:
3564 993 891 878 460 235 126 95
62 33
PmeI GTTT'AAAC
Cuts at: 4336 4336
Size: 7337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6476 6508 6476
Size: 32 7305
PpuMI rG'GwC_Cy
Cuts at: 207 467 2531 2556 2949 3565 3727 3745 207
Size: 260 2064 25 393 616 162 18 3799
Fragments arranged by size:
3799 2064 616 393 260 162 25 18
PshAI GACnn'nnGTC
Cuts at: 205 205
Size: 7337
PspOMI G'GGCC_C
Cuts at: 1847 3332 3832 1847
Size: 1485 500 5352
Fragments arranged by size:
5352 1485 500
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 499 531 499
Size: 32 7305
PstI C_TGCA'G
Cuts at: 224 1295 1461 2885 3162 3765 4384 5069 224
Size: 1071 166 1424 277 603 619 685 2492
Fragments arranged by size:
2492 1424 1071 685 619 603 277 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7337
PvuII CAG'CTG
Cuts at: 555 2548 2690 3036 3171 4762 5122 555
Size: 1993 142 346 135 1591 360 2770
Fragments arranged by size:
2770 1993 1591 360 346 142 135
RsrII CG'GwC_CG
Cuts at: 3635 5532 3635
Size: 1897 5440
SacI G_AGCT'C
Cuts at: 1705 3825 4351 1705
Size: 2120 526 4691
Fragments arranged by size:
4691 2120 526
SacII CC_GC'GG
Cuts at: 3347 3347
Size: 7337
SalI G'TCGA_C
Cuts at: 4374 4374
Size: 7337
SanDI GG'GwC_CC
Cuts at: 2556 3565 2556
Size: 1009 6328
SapI GCTCTTCn'nnn_
Cuts at: 1822 2824 5360 5570 1822
Size: 1002 2536 210 3589
Fragments arranged by size:
3589 2536 1002 210
SbfI CC_TGCA'GG
Cuts at: 2885 4384 2885
Size: 1499 5838
ScaI AGT'ACT
Cuts at: 6854 6854
Size: 7337
SexAI A'CCwGG_T
Cuts at: 965 965
Size: 7337
SfcI C'TryA_G
Cuts at: 33 220 598 1291 1457 2414 2881 3158 3351
Size: 187 378 693 166 957 467 277 193
Cuts at: 3351 3761 4380 5065 6058 6249 33
Size: 410 619 685 993 191 1121
Fragments arranged by size:
1121 993 957 693 685 619 467 410
378 277 193 191 187 166
SfoI GGC'GCC
Cuts at: 1974 3128 4769 5017 1974
Size: 1154 1641 248 4294
Fragments arranged by size:
4294 1641 1154 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7337
SgrAI Cr'CCGG_yG
Cuts at: 1969 1969
Size: 7337
SmaI CCC'GGG
Cuts at: 547 2089 2410 3548 4359 547
Size: 1542 321 1138 811 3525
Fragments arranged by size:
3525 1542 1138 811 321
SmlI C'TyrA_G
Cuts at: 2098 4492 5407 5899 6161 2098
Size: 2394 915 492 262 3274
Fragments arranged by size:
3274 2394 915 492 262
SnaBI TAC'GTA
Cuts at: 4329 4329
Size: 7337
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7337
SphI G_CATG'C
Cuts at: 1518 4390 4890 1518
Size: 2872 500 3965
Fragments arranged by size:
3965 2872 500
StuI AGG'CCT
Cuts at: 3949 4056 3949
Size: 107 7230
StyI C'CwwG_G
Cuts at: 203 986 1208 2973 4471 4825 203
Size: 783 222 1765 1498 354 2715
Fragments arranged by size:
2715 1765 1498 783 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 2295 2436 2887 3266 4147 4920 5053 5512
Size: 645 141 451 379 881 773 133 459
Cuts at: 5512 5721 1650
Size: 209 3266
Fragments arranged by size:
3266 881 773 645 459 451 379 209
141 133
TatI w'GTAC_w
Cuts at: 194 6852 194
Size: 6658 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 883 915 2677 2709 3086 3118 3807
Size: 32 310 32 1762 32 377 32 689
Cuts at: 3807 3839 3964 3996 6854 6886 541
Size: 32 125 32 2858 32 992
Fragments arranged by size:
2858 1762 992 689 377 310 125 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 715 3147 5134 715
Size: 2432 1987 2918
Fragments arranged by size:
2918 2432 1987
XbaI T'CTAG_A
Cuts at: 4368 4368
Size: 7337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 696 1408 1566 2013 2137 2155 2625 2820 4039
Size: 712 158 447 124 18 470 195 1219
Cuts at: 4039 4832 696
Size: 793 3201
Fragments arranged by size:
3201 1219 793 712 470 447 195 158
124 18
XmaI C'CCGG_G
Cuts at: 545 2087 2408 3546 4357 545
Size: 1542 321 1138 811 3525
Fragments arranged by size:
3525 1542 1138 811 321
ZraI GAC'GTC
Cuts at: 957 957
Size: 7337
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BcgI BfrBI
BmtI BsrGI BssHII BstZ17I CspCI EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI SfiI SrfI SspI SwaI
XhoI XmnI