Restriction map for
pF1KSDA1092
(Circular) (Six-base) MAPSORT of: pF1KSDA1092.seq Check: 2631 from: 1 to: 6520
pF1KSDA1092 6520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 3556 3556
Size: 6520
Acc65I G'GTAC_C
Cuts at: 866 1093 3534 866
Size: 227 2441 3852
Fragments arranged by size:
3852 2441 227
AclI AA'CG_TT
Cuts at: 2133 6360 2133
Size: 4227 2293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1640 2328 3136 4343 4775 5524 5792 785
Size: 855 688 808 1207 432 749 268 1513
Fragments arranged by size:
1513 1207 855 808 749 688 432 268
AflII C'TTAA_G
Cuts at: 1054 1054
Size: 6520
AflIII A'CryG_T
Cuts at: 3870 4976 5953 3870
Size: 1106 977 4437
Fragments arranged by size:
4437 1106 977
AgeI A'CCGG_T
Cuts at: 4908 4908
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 1997 3184 3823 5392 1997
Size: 1187 639 1569 3125
Fragments arranged by size:
3125 1569 1187 639
ApaI G_GGCC'C
Cuts at: 165 184 3035 165
Size: 19 2851 3650
Fragments arranged by size:
3650 2851 19
ApaLI G'TGCA_C
Cuts at: 2530 5290 2530
Size: 2760 3760
ApoI r'AATT_y
Cuts at: 596 1114 1154 1192 2020 2572 2644 3226 3522
Size: 518 40 38 828 552 72 582 296
Cuts at: 3522 5873 6129 596
Size: 2351 256 987
Fragments arranged by size:
2351 987 828 582 552 518 296 256
72 40 38
AseI AT'TA_AT
Cuts at: 20 545 20
Size: 525 5995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6520
AvaI C'yCGr_G
Cuts at: 177 329 404 3538 177
Size: 152 75 3134 3159
Fragments arranged by size:
3159 3134 152 75
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 850 883 1085 1118 850
Size: 33 202 33 6252
Fragments arranged by size:
6252 202 33 33
BamHI G'GATC_C
Cuts at: 3543 3543
Size: 6520
BanI G'GyrC_C
Cuts at: 148 193 202 866 1093 2443 3253 3427 3534
Size: 45 9 664 227 1350 810 174 107
Cuts at: 3534 3948 4196 4231 148
Size: 414 248 35 2437
Fragments arranged by size:
2437 1350 810 664 414 248 227 174
107 45 35 9
BanII G_rGCy'C
Cuts at: 165 184 1512 3035 3532 4562 165
Size: 19 1328 1523 497 1030 2123
Fragments arranged by size:
2123 1523 1328 1030 497 19
BbeI G_GCGC'C
Cuts at: 152 197 206 3952 4200 152
Size: 45 9 3746 248 2472
Fragments arranged by size:
3746 2472 248 45 9
BciVI GTATCCnnnnn_n'
Cuts at: 1673 4416 5179 6520 1673
Size: 2743 763 1341 1673
Fragments arranged by size:
2743 1673 1341 763
BclI T'GATC_A
Cuts at: 4038 4038
Size: 6520
BfrBI ATG'CAT
Cuts at: 1645 2842 1645
Size: 1197 5323
BglI GCCn_nnn'nGGC
Cuts at: 146 160 146
Size: 14 6506
BglII A'GATC_T
Cuts at: 2919 3339 4033 6072 2919
Size: 420 694 2039 3367
Fragments arranged by size:
3367 2039 694 420
BlpI GC'TnA_GC
Cuts at: 3630 3630
Size: 6520
Bme1580I G_kGCm'C
Cuts at: 165 184 2534 3035 4143 4236 5294 165
Size: 19 2350 501 1108 93 1058 1391
Fragments arranged by size:
2350 1391 1108 1058 501 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 2061 2150 3730 3811 4146 6292 2061
Size: 89 1580 81 335 2146 2289
Fragments arranged by size:
2289 2146 1580 335 89 81
BmtI G_CTAG'C
Cuts at: 338 338
Size: 6520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 265 1768 2352 4885 265
Size: 1503 584 2533 1900
Fragments arranged by size:
2533 1900 1503 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3521 2741
Size: 780 5740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2177 3142 3694 4573 5067 5365 1246
Size: 931 965 552 879 494 298 2401
Fragments arranged by size:
2401 965 931 879 552 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2340 2382 6224 2340
Size: 42 3842 2636
Fragments arranged by size:
3842 2636 42
BsaAI yAC'GTr
Cuts at: 2757 3510 3871 2757
Size: 753 361 5406
Fragments arranged by size:
5406 753 361
BsaBI GATnn'nnATC
Cuts at: 6 4032 6071 6
Size: 4026 2039 455
Fragments arranged by size:
4026 2039 455
BsaHI Gr'CG_yC
Cuts at: 149 194 203 3949 4197 4901 6397 149
Size: 45 9 3746 248 704 1496 272
Fragments arranged by size:
3746 1496 704 272 248 45 9
BsaWI w'CCGG_w
Cuts at: 3707 4228 4908 5182 5329 5740 3707
Size: 521 680 274 147 411 4487
Fragments arranged by size:
4487 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3494 3524 3494
Size: 30 6490
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2344 2792 2795 2967 2344
Size: 448 3 172 5897
Fragments arranged by size:
5897 448 172 3
BseYI C'CCAG_C
Cuts at: 379 2894 3943 4701 5280 5776 379
Size: 2515 1049 758 579 496 1123
Fragments arranged by size:
2515 1123 1049 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2528
Size: 6520
BsiEI CG_ry'CG
Cuts at: 104 141 389 4060 4106 5316 104
Size: 37 248 3671 46 1210 1308
Fragments arranged by size:
3671 1308 1210 248 46 37
BsiHKAI G_wGCw'C
Cuts at: 1512 2534 3532 4310 4500 5294 1512
Size: 1022 998 778 190 794 2738
Fragments arranged by size:
2738 1022 998 794 778 190
BsmI GAATG_Cn'
Cuts at: 1645 2895 2946 1645
Size: 1250 51 5219
Fragments arranged by size:
5219 1250 51
BsmBI CGTCTCn'nnnn_
Cuts at: 2537 2537
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 165 184 1512 2534 3035 3532 4143 4236 4310
Size: 19 1328 1022 501 497 611 93 74
Cuts at: 4310 4500 4562 5294 165
Size: 190 62 732 1391
Fragments arranged by size:
1391 1328 1022 732 611 501 497 190
93 74 62 19
BspEI T'CCGG_A
Cuts at: 5740 5740
Size: 6520
BspHI T'CATG_A
Cuts at: 1131 2321 5696 1131
Size: 1190 3375 1955
Fragments arranged by size:
3375 1955 1190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2594 3568 3782 4084 4465 2594
Size: 974 214 302 381 4649
Fragments arranged by size:
4649 974 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4810 4864 6350 6515 50
Size: 4760 54 1486 165 55
Fragments arranged by size:
4760 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 747 1012 4430 747
Size: 265 3418 2837
Fragments arranged by size:
3418 2837 265
BsrFI r'CCGG_y
Cuts at: 154 4516 4697 4908 154
Size: 4362 181 211 1766
Fragments arranged by size:
4362 1766 211 181
BssHII G'CGCG_C
Cuts at: 265 265
Size: 6520
BssSI C'ACGA_G
Cuts at: 4789 5149 4789
Size: 360 6160
BstAPI GCAn_nnn'nTGC
Cuts at: 838 1651 3895 838
Size: 813 2244 3463
Fragments arranged by size:
3463 2244 813
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6520
BstYI r'GATC_y
Cuts at: 1 2463 2919 3339 3543 4010 4033 4368 4614
Size: 2462 456 420 204 467 23 335 246
Cuts at: 4614 5628 5714 5726 6072 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2462 1014 467 456 449 420 346 335
246 204 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 640 640
Size: 6520
BtgI C'CryG_G
Cuts at: 125 255 354 2487 2600 2870 3221 125
Size: 130 99 2133 113 270 351 3424
Fragments arranged by size:
3424 2133 351 270 130 113 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4468 4649 4812 4468
Size: 181 163 6176
Fragments arranged by size:
6176 181 163
BtsI GCAGTG_nn'
Cuts at: 534 1623 3004 3299 3761 534
Size: 1089 1381 295 462 3293
Fragments arranged by size:
3293 1381 1089 462 295
DraI TTT'AAA
Cuts at: 1034 3517 1034
Size: 2483 4037
DrdI GACnn_nn'nnGTC
Cuts at: 4224 5084 4224
Size: 860 5660
EaeI y'GGCC_r
Cuts at: 138 252 2311 2597 2652 2984 4103 4277 4668
Size: 114 2059 286 55 332 1119 174 391
Cuts at: 4668 4695 6042 138
Size: 27 1347 616
Fragments arranged by size:
2059 1347 1119 616 391 332 286 174
114 55 27
EagI C'GGCC_G
Cuts at: 138 4103 138
Size: 3965 2555
EarI CTCTTCn'nnn_
Cuts at: 463 2769 4541 4751 463
Size: 2306 1772 210 2232
Fragments arranged by size:
2306 2232 1772 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 4726 5038 5184 6330 145
Size: 4581 312 146 1146 335
Fragments arranged by size:
4581 1146 335 312 146
EcoICRI GAG'CTC
Cuts at: 1510 3530 1510
Size: 2020 4500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1640 2328 2741 3136 3521 4343 4775 5524
Size: 855 688 413 395 385 822 432 749
Cuts at: 5524 5792 785
Size: 268 1513
Fragments arranged by size:
1513 855 822 749 688 432 413 395
385 268
EcoO109I rG'GnC_Cy
Cuts at: 161 162 349 1209 1480 2727 2802 3031 3657
Size: 1 187 860 271 1247 75 229 626
Cuts at: 3657 161
Size: 3024
Fragments arranged by size:
3024 1247 860 626 271 229 187 75
1
EcoRI G'AATT_C
Cuts at: 1192 2644 3226 3522 1192
Size: 1452 582 296 4190
Fragments arranged by size:
4190 1452 582 296
EcoRV GAT'ATC
Cuts at: 761 761
Size: 6520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1213 3358 4537 1213
Size: 2145 1179 3196
Fragments arranged by size:
3196 2145 1179
FspI TGC'GCA
Cuts at: 4299 4299
Size: 6520
HaeII r_GCGC'y
Cuts at: 152 197 206 2351 3952 4200 5224 6203 152
Size: 45 9 2145 1601 248 1024 979 469
Fragments arranged by size:
2145 1601 1024 979 469 248 45 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 676 708 745 777 4360 4390 4392 4422 676
Size: 32 37 32 3583 30 2 30 2774
Fragments arranged by size:
3583 2774 37 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3557 3557
Size: 6520
HindIII A'AGCT_T
Cuts at: 2565 2565
Size: 6520
KasI G'GCGC_C
Cuts at: 148 193 202 3948 4196 148
Size: 45 9 3746 248 2472
Fragments arranged by size:
3746 2472 248 45 9
KpnI G_GTAC'C
Cuts at: 870 1097 3538 870
Size: 227 2441 3852
Fragments arranged by size:
3852 2441 227
MluI A'CGCG_T
Cuts at: 5953 5953
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 611 1511 5191 5375 314
Size: 297 900 3680 184 1459
Fragments arranged by size:
3680 1459 900 297 184
MscI TGG'CCA
Cuts at: 2599 2986 4279 2599
Size: 387 1293 4840
Fragments arranged by size:
4840 1293 387
MslI CAynn'nnrTG
Cuts at: 1253 1286 2537 4507 4634 1253
Size: 33 1251 1970 127 3139
Fragments arranged by size:
3139 1970 1251 127 33
MspA1I CmG'CkG
Cuts at: 278 356 3629 3943 4303 5318 5563 278
Size: 78 3273 314 360 1015 245 1235
Fragments arranged by size:
3273 1235 1015 360 314 245 78
NaeI GCC'GGC
Cuts at: 156 4699 156
Size: 4543 1977
NarI GG'CG_CC
Cuts at: 149 194 203 3949 4197 149
Size: 45 9 3746 248 2472
Fragments arranged by size:
3746 2472 248 45 9
NcoI C'CATG_G
Cuts at: 125 2487 2600 3221 125
Size: 2362 113 621 3424
Fragments arranged by size:
3424 2362 621 113
NgoMIV G'CCGG_C
Cuts at: 154 4697 154
Size: 4543 1977
NheI G'CTAG_C
Cuts at: 334 334
Size: 6520
NruI TCG'CGA
Cuts at: 4881 4881
Size: 6520
NsiI A_TGCA'T
Cuts at: 1647 2844 1647
Size: 1197 5323
NspI r_CATG'y
Cuts at: 507 633 2740 2937 3006 3571 4071 4980 507
Size: 126 2107 197 69 565 500 909 2047
Fragments arranged by size:
2107 2047 909 565 500 197 126 69
PasI CC'CwG_GG
Cuts at: 2371 2371
Size: 6520
PciI A'CATG_T
Cuts at: 4976 4976
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3517 3517
Size: 6520
PmlI CAC'GTG
Cuts at: 2757 2757
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1042 1074 1456 1488 5659 5691 1042
Size: 32 382 32 4171 32 1871
Fragments arranged by size:
4171 1871 382 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 349 1209 1480 2727 349
Size: 860 271 1247 4142
Fragments arranged by size:
4142 1247 860 271
PsiI TTA'TAA
Cuts at: 1435 1435
Size: 6520
PspOMI G'GGCC_C
Cuts at: 161 180 3031 161
Size: 19 2851 3650
Fragments arranged by size:
3650 2851 19
PstI C_TGCA'G
Cuts at: 304 2711 3095 3565 4250 304
Size: 2407 384 470 685 2574
Fragments arranged by size:
2574 2407 685 470 384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6520
PvuII CAG'CTG
Cuts at: 3943 4303 3943
Size: 360 6160
RsrII CG'GwC_CG
Cuts at: 4713 4713
Size: 6520
SacI G_AGCT'C
Cuts at: 1512 3532 1512
Size: 2020 4500
SacII CC_GC'GG
Cuts at: 357 357
Size: 6520
SalI G'TCGA_C
Cuts at: 3555 3555
Size: 6520
SanDI GG'GwC_CC
Cuts at: 349 349
Size: 6520
SapI GCTCTTCn'nnn_
Cuts at: 4541 4751 4541
Size: 210 6310
SbfI CC_TGCA'GG
Cuts at: 3565 3565
Size: 6520
ScaI AGT'ACT
Cuts at: 2050 6037 2050
Size: 3987 2533
SfcI C'TryA_G
Cuts at: 33 300 2707 3091 3561 4246 5241 5432 33
Size: 267 2407 384 470 685 995 191 1121
Fragments arranged by size:
2407 1121 995 685 470 384 267 191
SfoI GGC'GCC
Cuts at: 150 195 204 3950 4198 150
Size: 45 9 3746 248 2472
Fragments arranged by size:
3746 2472 248 45 9
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 154 154
Size: 6520
SmaI CCC'GGG
Cuts at: 179 331 406 3540 179
Size: 152 75 3134 3159
Fragments arranged by size:
3159 3134 152 75
SmlI C'TyrA_G
Cuts at: 1054 1225 2192 3157 3673 4588 5082 5344 1054
Size: 171 967 965 516 915 494 262 2230
Fragments arranged by size:
2230 967 965 915 516 494 262 171
SnaBI TAC'GTA
Cuts at: 3510 3510
Size: 6520
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6520
SphI G_CATG'C
Cuts at: 507 3571 4071 507
Size: 3064 500 2956
Fragments arranged by size:
3064 2956 500
SrfI GCCC'GGGC
Cuts at: 331 331
Size: 6520
SspI AAT'ATT
Cuts at: 2268 3315 2268
Size: 1047 5473
StyI C'CwwG_G
Cuts at: 125 197 975 1205 2487 2600 3221 3257 3652
Size: 72 778 230 1282 113 621 36 395
Cuts at: 3652 4006 125
Size: 354 2639
Fragments arranged by size:
2639 1282 778 621 395 354 230 113
72 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1782 4101 4234 4693 4904 1782
Size: 2319 133 459 211 3398
Fragments arranged by size:
3398 2319 459 211 133
TatI w'GTAC_w
Cuts at: 1141 2048 2657 6035 1141
Size: 907 609 3378 1626
Fragments arranged by size:
3378 1626 907 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6037 6069 6037
Size: 32 6488
Tth111I GACn'n_nGTC
Cuts at: 2365 4315 2365
Size: 1950 4570
XbaI T'CTAG_A
Cuts at: 592 3549 592
Size: 2957 3563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2454 4013 2454
Size: 1559 4961
XmaI C'CCGG_G
Cuts at: 177 329 404 3538 177
Size: 152 75 3134 3159
Fragments arranged by size:
3159 3134 152 75
XmnI GAAnn'nnTTC
Cuts at: 1277 2172 1277
Size: 895 5625
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NsiI NspI PasI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BmgBI Bpu10I BsiWI BsrGI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NotI PacI PflMI PfoI
PshAI PspXI PsrI SexAI SfiI StuI SwaI XhoI
ZraI