Restriction map for
pF1KSDA1093
(Circular) (Six-base) MAPSORT of: pF1KSDA1093.seq Check: 701 from: 1 to: 8305
pF1KSDA1093 8305 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5004 5004
Size: 8305
AccI GT'mk_AC
Cuts at: 701 1561 5341 701
Size: 860 3780 3665
Fragments arranged by size:
3780 3665 860
Acc65I G'GTAC_C
Cuts at: 3843 5319 3843
Size: 1476 6829
AclI AA'CG_TT
Cuts at: 2675 4454 8145 2675
Size: 1779 3691 2835
Fragments arranged by size:
3691 2835 1779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2789 3212 3900 4451 6128 6560 7309 7577
Size: 1784 423 688 551 1677 432 749 268
Cuts at: 7577 1005
Size: 1733
Fragments arranged by size:
1784 1733 1677 749 688 551 432 423
268
AfeI AGC'GCT
Cuts at: 551 2091 5127 551
Size: 1540 3036 3729
Fragments arranged by size:
3729 3036 1540
AflII C'TTAA_G
Cuts at: 1272 1272
Size: 8305
AflIII A'CryG_T
Cuts at: 4904 5655 6761 7738 4904
Size: 751 1106 977 5471
Fragments arranged by size:
5471 1106 977 751
AgeI A'CCGG_T
Cuts at: 6693 6693
Size: 8305
AleI CACnn'nnGTG
Cuts at: 3436 4907 3436
Size: 1471 6834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1493 1525 1493
Size: 32 8273
AlwNI CAG_nnn'CTG
Cuts at: 514 1438 3732 5037 5608 7177 514
Size: 924 2294 1305 571 1569 1642
Fragments arranged by size:
2294 1642 1569 1305 924 571
ApaI G_GGCC'C
Cuts at: 2878 3572 3821 5177 2878
Size: 694 249 1356 6006
Fragments arranged by size:
6006 1356 694 249
ApaLI G'TGCA_C
Cuts at: 1124 3749 7075 1124
Size: 2625 3326 2354
Fragments arranged by size:
3326 2625 2354
ApoI r'AATT_y
Cuts at: 621 1223 1719 2862 3247 3450 3617 4228 5307
Size: 602 496 1143 385 203 167 611 1079
Cuts at: 5307 7658 7914 621
Size: 2351 256 1012
Fragments arranged by size:
2351 1143 1079 1012 611 602 496 385
256 203 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 8305
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8305
AvaI C'yCGr_G
Cuts at: 389 636 2885 3277 4076 5107 5323 389
Size: 247 2249 392 799 1031 216 3371
Fragments arranged by size:
3371 2249 1031 799 392 247 216
BamHI G'GATC_C
Cuts at: 5328 5328
Size: 8305
BanI G'GyrC_C
Cuts at: 396 658 2040 3843 4414 5319 5733 5981 6016
Size: 262 1382 1803 571 905 414 248 35
Cuts at: 6016 396
Size: 2685
Fragments arranged by size:
2685 1803 1382 905 571 414 262 248
35
BanII G_rGCy'C
Cuts at: 637 2174 2878 2988 3572 3821 3869 5108 5177
Size: 1537 704 110 584 249 48 1239 69
Cuts at: 5177 5317 6347 637
Size: 140 1030 2595
Fragments arranged by size:
2595 1537 1239 1030 704 584 249 140
110 69 48
BbeI G_GCGC'C
Cuts at: 5737 5985 5737
Size: 248 8057
BbsI GAAGACnn'nnnn_
Cuts at: 173 2345 173
Size: 2172 6133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 395 429 3408 3442 3718 3752 4779 4813 395
Size: 34 2979 34 276 34 1027 34 3887
Fragments arranged by size:
3887 2979 1027 276 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4267 6201 6964 8305 4267
Size: 1934 763 1341 4267
Fragments arranged by size:
4267 1934 1341 763
BclI T'GATC_A
Cuts at: 5823 5823
Size: 8305
BglI GCCn_nnn'nGGC
Cuts at: 293 293
Size: 8305
BglII A'GATC_T
Cuts at: 1804 5818 7857 1804
Size: 4014 2039 2252
Fragments arranged by size:
4014 2252 2039
BlpI GC'TnA_GC
Cuts at: 3601 4982 5415 3601
Size: 1381 433 6491
Fragments arranged by size:
6491 1381 433
Bme1580I G_kGCm'C
Cuts at: 245 1128 2878 3572 3753 3821 4846 5177 5928
Size: 883 1750 694 181 68 1025 331 751
Cuts at: 5928 6021 7079 245
Size: 93 1058 1471
Fragments arranged by size:
1750 1471 1058 1025 883 751 694 331
181 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 1391 1532 1679 2259 2735 3455 3480 3567 3618
Size: 141 147 580 476 720 25 87 51
Cuts at: 3618 4135 5111 5515 5596 5931 8077 1391
Size: 517 976 404 81 335 2146 1619
Fragments arranged by size:
2146 1619 976 720 580 517 476 404
335 147 141 87 81 51 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6670 6670
Size: 8305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1320 1388 1751 2651 2718 4208 4551 4653
Size: 784 68 363 900 67 1490 343 102
Cuts at: 4653 4725 5059 536
Size: 72 334 3782
Fragments arranged by size:
3782 1490 900 784 363 343 334 102
72 68 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 3686 4803 5479 6358 6852 7150 3505
Size: 181 1117 676 879 494 298 4660
Fragments arranged by size:
4660 1117 879 676 494 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 1647 2810 5035 8009 1647
Size: 1163 2225 2974 1943
Fragments arranged by size:
2974 2225 1943 1163
BsaAI yAC'GTr
Cuts at: 5295 5656 5295
Size: 361 7944
BsaBI GATnn'nnATC
Cuts at: 6 5817 7856 6
Size: 5811 2039 455
Fragments arranged by size:
5811 2039 455
BsaHI Gr'CG_yC
Cuts at: 2331 5734 5982 6686 8182 2331
Size: 3403 248 704 1496 2454
Fragments arranged by size:
3403 2454 1496 704 248
BsaWI w'CCGG_w
Cuts at: 5492 6013 6693 6967 7114 7525 5492
Size: 521 680 274 147 411 6272
Fragments arranged by size:
6272 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 1246 1276 1493 1523 4698 4728 646
Size: 30 570 30 217 30 3175 30 4223
Fragments arranged by size:
4223 3175 570 217 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 488 636 2270 3022 3025 3035 3572 5288 488
Size: 148 1634 752 3 10 537 1716 3505
Fragments arranged by size:
3505 1716 1634 752 537 148 10 3
BseYI C'CCAG_C
Cuts at: 1991 2120 2134 2302 2535 2877 2954 2995 2999
Size: 129 14 168 233 342 77 41 4
Cuts at: 2999 3077 4308 5728 6486 7065 7561 1991
Size: 78 1231 1420 758 579 496 2735
Fragments arranged by size:
2735 1420 1231 758 579 496 342 233
168 129 78 77 41 14 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 1424 3412 3460 4880 4885 489
Size: 935 1988 48 1420 5 3909
Fragments arranged by size:
3909 1988 1420 935 48 5
BsiEI CG_ry'CG
Cuts at: 104 281 1900 5845 5891 7101 104
Size: 177 1619 3945 46 1210 1308
Fragments arranged by size:
3945 1619 1308 1210 177 46
BsiHKAI G_wGCw'C
Cuts at: 814 1128 1345 1979 2174 2988 3112 3753 5317
Size: 314 217 634 195 814 124 641 1564
Cuts at: 5317 6095 6285 7079 814
Size: 778 190 794 2040
Fragments arranged by size:
2040 1564 814 794 778 641 634 314
217 195 190 124
BsmI GAATG_Cn'
Cuts at: 1310 1310
Size: 8305
BsmBI CGTCTCn'nnnn_
Cuts at: 2542 2542
Size: 8305
Bsp1286I G_dGCh'C
Cuts at: 245 637 814 1128 1345 1979 2174 2878 2988
Size: 392 177 314 217 634 195 704 110
Cuts at: 2988 3112 3572 3753 3821 3869 4846 5108 5177
Size: 124 460 181 68 48 977 262 69
Cuts at: 5177 5317 5928 6021 6095 6285 6347 7079 245
Size: 140 611 93 74 190 62 732 1471
Fragments arranged by size:
1471 977 732 704 634 611 460 392
314 262 217 195 190 181 177 140
124 110 93 74 69 68 62 48
BspEI T'CCGG_A
Cuts at: 7525 7525
Size: 8305
BspHI T'CATG_A
Cuts at: 3296 7481 3296
Size: 4185 4120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2906 4173 5004 5082 5353 5567 5869 6250 2906
Size: 1267 831 78 271 214 302 381 4961
Fragments arranged by size:
4961 1267 831 381 302 271 214 78
BsrBI CCG'CTC
Cuts at: 50 6595 6649 8135 8300 50
Size: 6545 54 1486 165 55
Fragments arranged by size:
6545 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1785 2493 4215 6215 1785
Size: 708 1722 2000 3875
Fragments arranged by size:
3875 2000 1722 708
BsrFI r'CCGG_y
Cuts at: 5044 6301 6482 6693 5044
Size: 1257 181 211 6656
Fragments arranged by size:
6656 1257 211 181
BssSI C'ACGA_G
Cuts at: 6574 6934 6574
Size: 360 7945
BstAPI GCAn_nnn'nTGC
Cuts at: 5680 5680
Size: 8305
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8305
BstEII G'GTnAC_C
Cuts at: 4085 4160 5260 4085
Size: 75 1100 7130
Fragments arranged by size:
7130 1100 75
BstXI CCAn_nnnn'nTGG
Cuts at: 342 1055 3342 342
Size: 713 2287 5305
Fragments arranged by size:
5305 2287 713
BstYI r'GATC_y
Cuts at: 1 1393 1804 2530 4506 5061 5220 5328 5795
Size: 1392 411 726 1976 555 159 108 467
Cuts at: 5795 5818 6153 6399 7413 7499 7511 7857 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1976 1392 1014 726 555 467 449 411
346 335 246 159 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 914 2051 2726 914
Size: 1137 675 6493
Fragments arranged by size:
6493 1137 675
BtgI C'CryG_G
Cuts at: 1289 4246 5066 1289
Size: 2957 820 4528
Fragments arranged by size:
4528 2957 820
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 290 3283 6253 6434 6597 290
Size: 2993 2970 181 163 1998
Fragments arranged by size:
2993 2970 1998 181 163
BtsI GCAGTG_nn'
Cuts at: 353 3057 3751 4810 4844 4897 5119 5546 353
Size: 2704 694 1059 34 53 222 427 3112
Fragments arranged by size:
3112 2704 1059 694 427 222 53 34
ClaI AT'CG_AT
Cuts at: 3309 3309
Size: 8305
DraI TTT'AAA
Cuts at: 3981 5302 3981
Size: 1321 6984
DraIII CAC_nnn'GTG
Cuts at: 432 2024 4129 4414 432
Size: 1592 2105 285 4323
Fragments arranged by size:
4323 2105 1592 285
DrdI GACnn_nn'nnGTC
Cuts at: 2974 4288 6009 6869 2974
Size: 1314 1721 860 4410
Fragments arranged by size:
4410 1721 1314 860
EaeI y'GGCC_r
Cuts at: 278 1057 1890 4131 4880 5046 5888 6062 6453
Size: 779 833 2241 749 166 842 174 391
Cuts at: 6453 6480 7827 278
Size: 27 1347 756
Fragments arranged by size:
2241 1347 842 833 779 756 749 391
174 166 27
EagI C'GGCC_G
Cuts at: 278 5888 278
Size: 5610 2695
EarI CTCTTCn'nnn_
Cuts at: 713 802 2030 2247 2747 2999 6326 6536 713
Size: 89 1228 217 500 252 3327 210 2482
Fragments arranged by size:
3327 2482 1228 500 252 217 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6511 6823 6969 8115 6511
Size: 312 146 1146 6701
Fragments arranged by size:
6701 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2172 2986 5315 2172
Size: 814 2329 5162
Fragments arranged by size:
5162 2329 814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1005 1320 1388 1751 2651 2718 2789 3212
Size: 469 315 68 363 900 67 71 423
Cuts at: 3212 3900 4208 4451 4551 4653 4725 5059 6128
Size: 688 308 243 100 102 72 334 1069
Cuts at: 6128 6560 7309 7577 536
Size: 432 749 268 1264
Fragments arranged by size:
1264 1069 900 749 688 469 432 423
363 334 315 308 268 243 102 100
72 71 68 67
EcoO109I rG'GnC_Cy
Cuts at: 493 1414 1571 2874 3568 3569 4141 5071 5173
Size: 921 157 1303 694 1 572 930 102
Cuts at: 5173 5174 5442 493
Size: 1 268 3356
Fragments arranged by size:
3356 1303 930 921 694 572 268 157
102 1 1
EcoRI G'AATT_C
Cuts at: 2862 4228 5307 2862
Size: 1366 1079 5860
Fragments arranged by size:
5860 1366 1079
EcoRV GAT'ATC
Cuts at: 3294 4107 3294
Size: 813 7492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2516 6322 2516
Size: 3806 4499
FspI TGC'GCA
Cuts at: 6084 6084
Size: 8305
HaeII r_GCGC'y
Cuts at: 374 553 2093 4055 5129 5162 5737 5985 7009
Size: 179 1540 1962 1074 33 575 248 1024
Cuts at: 7009 7988 374
Size: 979 691
Fragments arranged by size:
1962 1540 1074 1024 979 691 575 248
179 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 1420 1452 2044 2076 2923 2955 3132
Size: 32 652 32 592 32 847 32 177
Cuts at: 3132 3164 3291 3323 3537 3569 4261 4293 4474
Size: 32 127 32 214 32 692 32 181
Cuts at: 4474 4506 4702 4734 4809 4841 6145 6175 6177
Size: 32 196 32 75 32 1304 30 2
Cuts at: 6177 6207 736
Size: 30 2834
Fragments arranged by size:
2834 1304 847 692 652 592 214 196
181 177 127 75 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4778 5342 4778
Size: 564 7741
KasI G'GCGC_C
Cuts at: 5733 5981 5733
Size: 248 8057
KpnI G_GTAC'C
Cuts at: 3847 5323 3847
Size: 1476 6829
MfeI C'AATT_G
Cuts at: 290 2403 290
Size: 2113 6192
MluI A'CGCG_T
Cuts at: 7738 7738
Size: 8305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 962 1371 1812 2270 2758 4257 4562 5261
Size: 288 409 441 458 488 1499 305 699
Cuts at: 5261 6976 7160 674
Size: 1715 184 1819
Fragments arranged by size:
1819 1715 1499 699 488 458 441 409
305 288 184
MscI TGG'CCA
Cuts at: 1059 4133 6064 1059
Size: 3074 1931 3300
Fragments arranged by size:
3300 3074 1931
MslI CAynn'nnrTG
Cuts at: 2908 3436 4046 4907 4909 6292 6419 2908
Size: 528 610 861 2 1383 127 4794
Fragments arranged by size:
4794 1383 861 610 528 127 2
MspA1I CmG'CkG
Cuts at: 2696 2705 2999 3732 4584 5020 5141 5414 5728
Size: 9 294 733 852 436 121 273 314
Cuts at: 5728 6088 7103 7348 2696
Size: 360 1015 245 3653
Fragments arranged by size:
3653 1015 852 733 436 360 314 294
273 245 121 9
NaeI GCC'GGC
Cuts at: 6484 6484
Size: 8305
NarI GG'CG_CC
Cuts at: 5734 5982 5734
Size: 248 8057
NcoI C'CATG_G
Cuts at: 1289 4246 1289
Size: 2957 5348
NgoMIV G'CCGG_C
Cuts at: 6482 6482
Size: 8305
NruI TCG'CGA
Cuts at: 6666 6666
Size: 8305
NspI r_CATG'y
Cuts at: 387 474 3641 4800 4908 5356 5856 6765 387
Size: 87 3167 1159 108 448 500 909 1927
Fragments arranged by size:
3167 1927 1159 909 500 448 108 87
PasI CC'CwG_GG
Cuts at: 1970 4839 1970
Size: 2869 5436
PciI A'CATG_T
Cuts at: 4904 6761 4904
Size: 1857 6448
PflMI CCAn_nnn'nTGG
Cuts at: 319 1249 2570 2747 3898 4600 5232 319
Size: 930 1321 177 1151 702 632 3392
Fragments arranged by size:
3392 1321 1151 930 702 632 177
PfoI T'CCnGG_A
Cuts at: 1336 1492 1573 2569 4567 1336
Size: 156 81 996 1998 5074
Fragments arranged by size:
5074 1998 996 156 81
PmeI GTTT'AAAC
Cuts at: 5302 5302
Size: 8305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 784 816 1493 1525 2224 2256 3957 3989 7444
Size: 32 677 32 699 32 1701 32 3455
Cuts at: 7444 7476 784
Size: 32 1613
Fragments arranged by size:
3455 1701 1613 699 677 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 1571 4141 5071 1571
Size: 2570 930 4805
Fragments arranged by size:
4805 2570 930
PsiI TTA'TAA
Cuts at: 2831 2831
Size: 8305
PspOMI G'GGCC_C
Cuts at: 2874 3568 3817 5173 2874
Size: 694 249 1356 6006
Fragments arranged by size:
6006 1356 694 249
PstI C_TGCA'G
Cuts at: 560 3758 5001 5350 6035 560
Size: 3198 1243 349 685 2830
Fragments arranged by size:
3198 2830 1243 685 349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8305
PvuII CAG'CTG
Cuts at: 2696 2999 3732 5020 5728 6088 2696
Size: 303 733 1288 708 360 4913
Fragments arranged by size:
4913 1288 733 708 360 303
RsrII CG'GwC_CG
Cuts at: 6498 6498
Size: 8305
SacI G_AGCT'C
Cuts at: 2174 2988 5317 2174
Size: 814 2329 5162
Fragments arranged by size:
5162 2329 814
SalI G'TCGA_C
Cuts at: 5340 5340
Size: 8305
SapI GCTCTTCn'nnn_
Cuts at: 802 2747 6326 6536 802
Size: 1945 3579 210 2571
Fragments arranged by size:
3579 2571 1945 210
SbfI CC_TGCA'GG
Cuts at: 5350 5350
Size: 8305
ScaI AGT'ACT
Cuts at: 7822 7822
Size: 8305
SexAI A'CCwGG_T
Cuts at: 4410 5256 4410
Size: 846 7459
SfcI C'TryA_G
Cuts at: 33 556 947 2183 3224 3754 4016 4055 4425
Size: 523 391 1236 1041 530 262 39 370
Cuts at: 4425 4997 5346 6031 7026 7217 33
Size: 572 349 685 995 191 1121
Fragments arranged by size:
1236 1121 1041 995 685 572 530 523
391 370 349 262 191 39
SfoI GGC'GCC
Cuts at: 5735 5983 5735
Size: 248 8057
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8305
SmaI CCC'GGG
Cuts at: 4078 5325 4078
Size: 1247 7058
SmlI C'TyrA_G
Cuts at: 1272 2885 3520 3701 4782 5458 6373 6867 7129
Size: 1613 635 181 1081 676 915 494 262
Cuts at: 7129 1272
Size: 2448
Fragments arranged by size:
2448 1613 1081 915 676 635 494 262
181
SnaBI TAC'GTA
Cuts at: 5295 5295
Size: 8305
SpeI A'CTAG_T
Cuts at: 67 832 67
Size: 765 7540
SphI G_CATG'C
Cuts at: 474 4800 5356 5856 474
Size: 4326 556 500 2923
Fragments arranged by size:
4326 2923 556 500
StuI AGG'CCT
Cuts at: 2049 2049
Size: 8305
StyI C'CwwG_G
Cuts at: 477 591 1289 1773 2044 2868 4246 4883 5437
Size: 114 698 484 271 824 1378 637 554
Cuts at: 5437 5791 477
Size: 354 2991
Fragments arranged by size:
2991 1378 824 698 637 554 484 354
271 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1886 2148 2426 2501 4117 5886 6019 6478 6689
Size: 262 278 75 1616 1769 133 459 211
Cuts at: 6689 1886
Size: 3502
Fragments arranged by size:
3502 1769 1616 459 278 262 211 133
75
TatI w'GTAC_w
Cuts at: 7820 7820
Size: 8305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 972 1004 2008 2040 2302 2334 4698 4730 7822
Size: 32 1004 32 262 32 2364 32 3092
Cuts at: 7822 7854 972
Size: 32 1423
Fragments arranged by size:
3092 2364 1423 1004 262 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1432 1671 6100 1432
Size: 239 4429 3637
Fragments arranged by size:
4429 3637 239
XbaI T'CTAG_A
Cuts at: 5334 5334
Size: 8305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 680 3828 4244 4628 5024 5798 680
Size: 3148 416 384 396 774 3187
Fragments arranged by size:
3187 3148 774 416 396 384
XhoI C'TCGA_G
Cuts at: 2885 2885
Size: 8305
XmaI C'CCGG_G
Cuts at: 4076 5323 4076
Size: 1247 7058
XmnI GAAnn'nnTTC
Cuts at: 1806 3234 3696 1806
Size: 1428 462 6415
Fragments arranged by size:
6415 1428 462
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AscI AvrII BaeI BbvCI BfrBI
BmgBI BmtI Bpu10I BsiWI BsrGI BssHII BstZ17I CspCI
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NsiI PacI PmlI PshAI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI ZraI