Restriction map for
pF1KSDA1094
(Circular) (Six-base) MAPSORT of: pF1KSDA1094.seq Check: 4081 from: 1 to: 4753
pF1KSDA1094 4753 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1789 1789
Size: 4753
Acc65I G'GTAC_C
Cuts at: 1767 1767
Size: 4753
AclI AA'CG_TT
Cuts at: 4593 4593
Size: 4753
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 973 2576 3008 3757 4025 831
Size: 142 1603 432 749 268 1559
Fragments arranged by size:
1603 1559 749 432 268 142
AfeI AGC'GCT
Cuts at: 757 757
Size: 4753
AflIII A'CryG_T
Cuts at: 2103 3209 4186 2103
Size: 1106 977 2670
Fragments arranged by size:
2670 1106 977
AgeI A'CCGG_T
Cuts at: 3141 3141
Size: 4753
AlwNI CAG_nnn'CTG
Cuts at: 475 1400 2056 3625 475
Size: 925 656 1569 1603
Fragments arranged by size:
1603 1569 925 656
ApaI G_GGCC'C
Cuts at: 1437 1437
Size: 4753
ApaLI G'TGCA_C
Cuts at: 3523 3523
Size: 4753
ApoI r'AATT_y
Cuts at: 1755 4106 4362 1755
Size: 2351 256 2146
Fragments arranged by size:
2351 2146 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4753
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4753
AvaI C'yCGr_G
Cuts at: 131 153 1771 131
Size: 22 1618 3113
Fragments arranged by size:
3113 1618 22
BamHI G'GATC_C
Cuts at: 1776 1776
Size: 4753
BanI G'GyrC_C
Cuts at: 419 877 1767 2181 2429 2464 419
Size: 458 890 414 248 35 2708
Fragments arranged by size:
2708 890 458 414 248 35
BanII G_rGCy'C
Cuts at: 168 1437 1765 2795 168
Size: 1269 328 1030 2126
Fragments arranged by size:
2126 1269 1030 328
BbeI G_GCGC'C
Cuts at: 2185 2433 2185
Size: 248 4505
BbsI GAAGACnn'nnnn_
Cuts at: 327 855 1224 327
Size: 528 369 3856
Fragments arranged by size:
3856 528 369
BciVI GTATCCnnnnn_n'
Cuts at: 2649 3412 4753 2649
Size: 763 1341 2649
Fragments arranged by size:
2649 1341 763
BclI T'GATC_A
Cuts at: 2271 2271
Size: 4753
BglI GCCn_nnn'nGGC
Cuts at: 1054 1054
Size: 4753
BglII A'GATC_T
Cuts at: 2266 4305 2266
Size: 2039 2714
BlpI GC'TnA_GC
Cuts at: 220 922 1863 220
Size: 702 941 3110
Fragments arranged by size:
3110 941 702
Bme1580I G_kGCm'C
Cuts at: 142 1437 2376 2469 3527 142
Size: 1295 939 93 1058 1368
Fragments arranged by size:
1368 1295 1058 939 93
BmrI ACTGGGnnnn_n'
Cuts at: 523 784 1963 2044 2379 4525 523
Size: 261 1179 81 335 2146 751
Fragments arranged by size:
2146 1179 751 335 261 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3118 3118
Size: 4753
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 1264 587
Size: 677 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 2806 3300 3598 1927
Size: 879 494 298 3082
Fragments arranged by size:
3082 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1043 4457 1043
Size: 3414 1339
BsaAI yAC'GTr
Cuts at: 1743 2104 1743
Size: 361 4392
BsaBI GATnn'nnATC
Cuts at: 6 1597 2265 4304 6
Size: 1591 668 2039 455
Fragments arranged by size:
2039 1591 668 455
BsaHI Gr'CG_yC
Cuts at: 2182 2430 3134 4630 2182
Size: 248 704 1496 2305
Fragments arranged by size:
2305 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1940 2461 3141 3415 3562 3973 1940
Size: 521 680 274 147 411 2720
Fragments arranged by size:
2720 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 879 1439 879
Size: 560 4193
BseYI C'CCAG_C
Cuts at: 430 2176 2934 3513 4009 430
Size: 1746 758 579 496 1174
Fragments arranged by size:
1746 1174 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 934 1386 298
Size: 636 452 3665
Fragments arranged by size:
3665 636 452
BsiEI CG_ry'CG
Cuts at: 104 2293 2339 3549 104
Size: 2189 46 1210 1308
Fragments arranged by size:
2189 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1765 2543 2733 3527 1765
Size: 778 190 794 2991
Fragments arranged by size:
2991 794 778 190
BsmI GAATG_Cn'
Cuts at: 1086 1086
Size: 4753
Bsp1286I G_dGCh'C
Cuts at: 142 168 1437 1765 2376 2469 2543 2733 2795
Size: 26 1269 328 611 93 74 190 62
Cuts at: 2795 3527 142
Size: 732 1368
Fragments arranged by size:
1368 1269 732 611 328 190 93 74
62 26
BspEI T'CCGG_A
Cuts at: 3973 3973
Size: 4753
BspHI T'CATG_A
Cuts at: 407 587 908 3929 407
Size: 180 321 3021 1231
Fragments arranged by size:
3021 1231 321 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 683 1364 1801 2015 2317 2698 683
Size: 681 437 214 302 381 2738
Fragments arranged by size:
2738 681 437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 417 3043 3097 4583 4748 50
Size: 367 2626 54 1486 165 55
Fragments arranged by size:
2626 1486 367 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2663 2663
Size: 4753
BsrFI r'CCGG_y
Cuts at: 308 953 1190 1450 2749 2930 3141 308
Size: 645 237 260 1299 181 211 1920
Fragments arranged by size:
1920 1299 645 260 237 211 181
BssSI C'ACGA_G
Cuts at: 252 3022 3382 252
Size: 2770 360 1623
Fragments arranged by size:
2770 1623 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2128 2128
Size: 4753
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4753
BstXI CCAn_nnnn'nTGG
Cuts at: 430 480 430
Size: 50 4703
BstYI r'GATC_y
Cuts at: 1 1514 1776 2243 2266 2601 2847 3861 3947
Size: 1513 262 467 23 335 246 1014 86
Cuts at: 3947 3959 4305 1
Size: 12 346 449
Fragments arranged by size:
1513 1014 467 449 346 335 262 246
86 23 12
BtgI C'CryG_G
Cuts at: 263 380 1505 263
Size: 117 1125 3511
Fragments arranged by size:
3511 1125 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1113 2701 2882 3045 1113
Size: 1588 181 163 2821
Fragments arranged by size:
2821 1588 181 163
BtsI GCAGTG_nn'
Cuts at: 218 281 491 706 1195 1994 218
Size: 63 210 215 489 799 2977
Fragments arranged by size:
2977 799 489 215 210 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 283 318 283
Size: 35 4718
DraI TTT'AAA
Cuts at: 1750 1750
Size: 4753
DrdI GACnn_nn'nnGTC
Cuts at: 2457 3317 2457
Size: 860 3893
EaeI y'GGCC_r
Cuts at: 1046 1192 2336 2510 2901 2928 4275 1046
Size: 146 1144 174 391 27 1347 1524
Fragments arranged by size:
1524 1347 1144 391 174 146 27
EagI C'GGCC_G
Cuts at: 2336 2336
Size: 4753
EarI CTCTTCn'nnn_
Cuts at: 499 1003 1379 2774 2984 499
Size: 504 376 1395 210 2268
Fragments arranged by size:
2268 1395 504 376 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2959 3271 3417 4563 2959
Size: 312 146 1146 3149
Fragments arranged by size:
3149 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1763 1763
Size: 4753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 831 973 1264 2576 3008 3757 4025 587
Size: 244 142 291 1312 432 749 268 1315
Fragments arranged by size:
1315 1312 749 432 291 268 244 142
EcoO109I rG'GnC_Cy
Cuts at: 157 786 1119 1433 1434 1890 157
Size: 629 333 314 1 456 3020
Fragments arranged by size:
3020 629 456 333 314 1
EcoRI G'AATT_C
Cuts at: 1755 1755
Size: 4753
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4753
FspI TGC'GCA
Cuts at: 1571 2532 1571
Size: 961 3792
HaeII r_GCGC'y
Cuts at: 332 514 759 2185 2433 3457 4436 332
Size: 182 245 1426 248 1024 979 649
Fragments arranged by size:
1426 1024 979 649 248 245 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 235 267 560 592 1683 1715 2593
Size: 32 76 32 293 32 1091 32 878
Cuts at: 2593 2623 2625 2655 127
Size: 30 2 30 2225
Fragments arranged by size:
2225 1091 878 293 76 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1790 1790
Size: 4753
KasI G'GCGC_C
Cuts at: 2181 2429 2181
Size: 248 4505
KpnI G_GTAC'C
Cuts at: 1771 1771
Size: 4753
MluI A'CGCG_T
Cuts at: 4186 4186
Size: 4753
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3424 3608 3424
Size: 184 4569
MscI TGG'CCA
Cuts at: 1048 2512 1048
Size: 1464 3289
MslI CAynn'nnrTG
Cuts at: 385 463 478 484 2740 2867 385
Size: 78 15 6 2256 127 2271
Fragments arranged by size:
2271 2256 127 78 15 6
MspA1I CmG'CkG
Cuts at: 171 686 1520 1862 2176 2536 3551 3796 171
Size: 515 834 342 314 360 1015 245 1128
Fragments arranged by size:
1128 1015 834 515 360 342 314 245
NaeI GCC'GGC
Cuts at: 2932 2932
Size: 4753
NarI GG'CG_CC
Cuts at: 2182 2430 2182
Size: 248 4505
NcoI C'CATG_G
Cuts at: 380 1505 380
Size: 1125 3628
NgoMIV G'CCGG_C
Cuts at: 2930 2930
Size: 4753
NruI TCG'CGA
Cuts at: 3114 3114
Size: 4753
NspI r_CATG'y
Cuts at: 1371 1804 2304 3213 1371
Size: 433 500 909 2911
Fragments arranged by size:
2911 909 500 433
PciI A'CATG_T
Cuts at: 3209 3209
Size: 4753
PfoI T'CCnGG_A
Cuts at: 626 1241 1670 626
Size: 615 429 3709
Fragments arranged by size:
3709 615 429
PmeI GTTT'AAAC
Cuts at: 1750 1750
Size: 4753
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3892 3924 3892
Size: 32 4721
PpuMI rG'GwC_Cy
Cuts at: 786 786
Size: 4753
PspOMI G'GGCC_C
Cuts at: 1433 1433
Size: 4753
PstI C_TGCA'G
Cuts at: 1798 2483 1798
Size: 685 4068
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4753
PvuII CAG'CTG
Cuts at: 2176 2536 2176
Size: 360 4393
RsrII CG'GwC_CG
Cuts at: 2946 2946
Size: 4753
SacI G_AGCT'C
Cuts at: 1765 1765
Size: 4753
SalI G'TCGA_C
Cuts at: 1788 1788
Size: 4753
SanDI GG'GwC_CC
Cuts at: 786 786
Size: 4753
SapI GCTCTTCn'nnn_
Cuts at: 2774 2984 2774
Size: 210 4543
SbfI CC_TGCA'GG
Cuts at: 1798 1798
Size: 4753
ScaI AGT'ACT
Cuts at: 4270 4270
Size: 4753
SfcI C'TryA_G
Cuts at: 33 316 1583 1626 1794 2479 3474 3665 33
Size: 283 1267 43 168 685 995 191 1121
Fragments arranged by size:
1267 1121 995 685 283 191 168 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 1054 1054
Size: 4753
SfoI GGC'GCC
Cuts at: 2183 2431 2183
Size: 248 4505
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4753
SmaI CCC'GGG
Cuts at: 155 1773 155
Size: 1618 3135
SmlI C'TyrA_G
Cuts at: 1906 2821 3315 3577 1906
Size: 915 494 262 3082
Fragments arranged by size:
3082 915 494 262
SnaBI TAC'GTA
Cuts at: 1743 1743
Size: 4753
SpeI A'CTAG_T
Cuts at: 67 402 67
Size: 335 4418
SphI G_CATG'C
Cuts at: 1804 2304 1804
Size: 500 4253
StyI C'CwwG_G
Cuts at: 380 927 1052 1505 1885 2239 380
Size: 547 125 453 380 354 2894
Fragments arranged by size:
2894 547 453 380 354 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 260 709 1456 1462 2334 2467 2926 3137 260
Size: 449 747 6 872 133 459 211 1876
Fragments arranged by size:
1876 872 747 459 449 211 133 6
TatI w'GTAC_w
Cuts at: 4268 4268
Size: 4753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4270 4302 4270
Size: 32 4721
Tth111I GACn'n_nGTC
Cuts at: 2548 2548
Size: 4753
XbaI T'CTAG_A
Cuts at: 1782 1782
Size: 4753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1221 2246 1221
Size: 1025 3728
XmaI C'CCGG_G
Cuts at: 153 1771 153
Size: 1618 3135
XmnI GAAnn'nnTTC
Cuts at: 439 635 439
Size: 196 4557
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SgrAI
SrfI SspI StuI SwaI XhoI ZraI