Restriction map for
pF1KSDA1095
(Circular) (Six-base) MAPSORT of: pF1KSDA1095.seq Check: 6061 from: 1 to: 6335
pF1KSDA1095 6335 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1116 1954 1116
Size: 838 5497
AccI GT'mk_AC
Cuts at: 3293 3323 3373 3293
Size: 30 50 6255
Fragments arranged by size:
6255 50 30
Acc65I G'GTAC_C
Cuts at: 3351 3351
Size: 6335
AclI AA'CG_TT
Cuts at: 6175 6175
Size: 6335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1968 2623 2989 4160 4592 5339 5607 193
Size: 1775 655 366 1171 432 747 268 921
Fragments arranged by size:
1775 1171 921 747 655 432 366 268
AfeI AGC'GCT
Cuts at: 436 620 2059 436
Size: 184 1439 4712
Fragments arranged by size:
4712 1439 184
AflIII A'CryG_T
Cuts at: 521 3687 4791 5768 521
Size: 3166 1104 977 1088
Fragments arranged by size:
3166 1104 1088 977
AgeI A'CCGG_T
Cuts at: 4723 4723
Size: 6335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2344 2344
Size: 6335
AlwNI CAG_nnn'CTG
Cuts at: 417 486 821 2628 2688 3069 3640 5207 417
Size: 69 335 1807 60 381 571 1567 1545
Fragments arranged by size:
1807 1567 1545 571 381 335 69 60
ApaLI G'TGCA_C
Cuts at: 5105 5105
Size: 6335
ApoI r'AATT_y
Cuts at: 781 1022 3339 5688 5944 781
Size: 241 2317 2349 256 1172
Fragments arranged by size:
2349 2317 1172 256 241
AseI AT'TA_AT
Cuts at: 20 1544 20
Size: 1524 4811
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
AvaI C'yCGr_G
Cuts at: 430 2388 2490 3355 430
Size: 1958 102 865 3410
Fragments arranged by size:
3410 1958 865 102
BamHI G'GATC_C
Cuts at: 1269 2036 3360 1269
Size: 767 1324 4244
Fragments arranged by size:
4244 1324 767
BanI G'GyrC_C
Cuts at: 2578 3351 3765 4013 4048 2578
Size: 773 414 248 35 4865
Fragments arranged by size:
4865 773 414 248 35
BanII G_rGCy'C
Cuts at: 320 1115 1876 2072 2081 2345 2363 2671 2857
Size: 795 761 196 9 264 18 308 186
Cuts at: 2857 3349 4379 320
Size: 492 1030 2276
Fragments arranged by size:
2276 1030 795 761 492 308 264 196
186 18 9
BbeI G_GCGC'C
Cuts at: 3769 4017 3769
Size: 248 6087
BbsI GAAGACnn'nnnn_
Cuts at: 225 1457 1781 225
Size: 1232 324 4779
Fragments arranged by size:
4779 1232 324
BbvCI CC'TCA_GC
Cuts at: 1299 1299
Size: 6335
BciVI GTATCCnnnnn_n'
Cuts at: 984 4233 4994 6335 984
Size: 3249 761 1341 984
Fragments arranged by size:
3249 1341 984 761
BclI T'GATC_A
Cuts at: 3855 3855
Size: 6335
BglI GCCn_nnn'nGGC
Cuts at: 557 730 557
Size: 173 6162
BglII A'GATC_T
Cuts at: 2441 3850 5887 2441
Size: 1409 2037 2889
Fragments arranged by size:
2889 2037 1409
BlpI GC'TnA_GC
Cuts at: 814 3447 814
Size: 2633 3702
Bme1580I G_kGCm'C
Cuts at: 2581 3960 4053 5109 2581
Size: 1379 93 1056 3807
Fragments arranged by size:
3807 1379 1056 93
BmgBI CAC'GTC
Cuts at: 159 231 327 501 2328 159
Size: 72 96 174 1827 4166
Fragments arranged by size:
4166 1827 174 96 72
BmrI ACTGGGnnnn_n'
Cuts at: 3547 3628 3963 6107 3547
Size: 81 335 2144 3775
Fragments arranged by size:
3775 2144 335 81
BmtI G_CTAG'C
Cuts at: 1748 1748
Size: 6335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1314 4702 1314
Size: 3388 2947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 628 1390 1396 1624 1729 2095 2200 2236
Size: 408 762 6 228 105 366 105 36
Cuts at: 2236 2458 3063 220
Size: 222 605 3492
Fragments arranged by size:
3492 762 605 408 366 228 222 105
105 36 6
Bpu10I CC'TnA_GC
Cuts at: 1299 2224 2851 1299
Size: 925 627 4783
Fragments arranged by size:
4783 925 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 337 661 766 2065 2242 2481 3118 3511
Size: 15 324 105 1299 177 239 637 393
Cuts at: 3511 4390 4882 5180 322
Size: 879 492 298 1477
Fragments arranged by size:
1477 1299 879 637 492 393 324 298
239 177 105 15
BsaI GGTCTCn'nnnn_
Cuts at: 1520 6039 1520
Size: 4519 1816
BsaAI yAC'GTr
Cuts at: 3327 3688 3327
Size: 361 5974
BsaBI GATnn'nnATC
Cuts at: 6 1274 1468 3241 3849 5886 6
Size: 1268 194 1773 608 2037 455
Fragments arranged by size:
2037 1773 1268 608 455 194
BsaHI Gr'CG_yC
Cuts at: 1901 2021 2141 3766 4014 4716 6212 1901
Size: 120 120 1625 248 702 1496 2024
Fragments arranged by size:
2024 1625 1496 702 248 120 120
BsaWI w'CCGG_w
Cuts at: 1436 3524 4045 4723 4997 5144 5555 1436
Size: 2088 521 678 274 147 411 2216
Fragments arranged by size:
2216 2088 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 2068 2098 2692 2722 880
Size: 30 1158 30 594 30 4493
Fragments arranged by size:
4493 1158 594 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1303 1378 1726 2478 3067 1303
Size: 75 348 752 589 4571
Fragments arranged by size:
4571 752 589 348 75
BseYI C'CCAG_C
Cuts at: 697 725 1252 1416 2677 2688 2756 3760 4518
Size: 28 527 164 1261 11 68 1004 758
Cuts at: 4518 5095 5591 697
Size: 577 496 1441
Fragments arranged by size:
1441 1261 1004 758 577 527 496 164
68 28 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 649 1144 1579 1678 1998 2272 286
Size: 363 495 435 99 320 274 4349
Fragments arranged by size:
4349 495 435 363 320 274 99
BsiEI CG_ry'CG
Cuts at: 104 300 396 928 2009 2801 3877 3923 5131
Size: 196 96 532 1081 792 1076 46 1208
Cuts at: 5131 104
Size: 1308
Fragments arranged by size:
1308 1208 1081 1076 792 532 196 96
46
BsiHKAI G_wGCw'C
Cuts at: 320 428 1858 1876 2072 2081 2345 2363 2857
Size: 108 1430 18 196 9 264 18 494
Cuts at: 2857 3349 4127 4317 5109 320
Size: 492 778 190 792 1546
Fragments arranged by size:
1546 1430 792 778 494 492 264 196
190 108 18 18 9
BsiWI C'GTAC_G
Cuts at: 374 374
Size: 6335
Bsp1286I G_dGCh'C
Cuts at: 320 428 1115 1858 1876 2072 2081 2345 2363
Size: 108 687 743 18 196 9 264 18
Cuts at: 2363 2581 2671 2857 3349 3960 4053 4127 4317
Size: 218 90 186 492 611 93 74 190
Cuts at: 4317 4379 5109 320
Size: 62 730 1546
Fragments arranged by size:
1546 743 730 687 611 492 264 218
196 190 186 108 93 90 74 62
18 18 9
BspEI T'CCGG_A
Cuts at: 5555 5555
Size: 6335
BspHI T'CATG_A
Cuts at: 1026 1074 3107 5511 1026
Size: 48 2033 2404 1850
Fragments arranged by size:
2404 2033 1850 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1116 1700 1954 1985 2711 3110 3385 3599 3901
Size: 584 254 31 726 399 275 214 302
Cuts at: 3901 4282 1116
Size: 381 3169
Fragments arranged by size:
3169 726 584 399 381 302 275 254
214 31
BsrBI CCG'CTC
Cuts at: 50 336 2430 2470 2643 4627 4681 6165 6330
Size: 286 2094 40 173 1984 54 1484 165
Cuts at: 6330 50
Size: 55
Fragments arranged by size:
2094 1984 1484 286 173 165 55 54
40
BsrDI GCAATG_nn'
Cuts at: 1493 1615 4247 1493
Size: 122 2632 3581
Fragments arranged by size:
3581 2632 122
BsrFI r'CCGG_y
Cuts at: 241 923 2143 4333 4514 4723 241
Size: 682 1220 2190 181 209 1853
Fragments arranged by size:
2190 1853 1220 682 209 181
BssHII G'CGCG_C
Cuts at: 179 286 454 591 611 638 659 693 797
Size: 107 168 137 20 27 21 34 104
Cuts at: 797 799 2235 2961 179
Size: 2 1436 726 3553
Fragments arranged by size:
3553 1436 726 168 137 107 104 34
27 21 20 2
BssSI C'ACGA_G
Cuts at: 1819 2338 4606 4964 1819
Size: 519 2268 358 3190
Fragments arranged by size:
3190 2268 519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 417 2486 3712 417
Size: 2069 1226 3040
Fragments arranged by size:
3040 2069 1226
BstBI TT'CG_AA
Cuts at: 110 4696 110
Size: 4586 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1546 1923 1546
Size: 377 5958
BstYI r'GATC_y
Cuts at: 1 1269 2036 2441 2565 2897 2972 3360 3827
Size: 1268 767 405 124 332 75 388 467
Cuts at: 3827 3850 4185 4431 5443 5529 5541 5887 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1268 1012 767 467 449 405 388 346
335 332 246 124 86 75 23 12
BstZ17I GTA'TAC
Cuts at: 3294 3324 3294
Size: 30 6305
BtgI C'CryG_G
Cuts at: 125 294 734 2516 2801 3261 125
Size: 169 440 1782 285 460 3199
Fragments arranged by size:
3199 1782 460 440 285 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4285 4466 4629 4285
Size: 181 163 5991
Fragments arranged by size:
5991 181 163
BtsI GCAGTG_nn'
Cuts at: 603 3578 603
Size: 2975 3360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 454 489 454
Size: 35 6300
DraI TTT'AAA
Cuts at: 3334 3334
Size: 6335
DraIII CAC_nnn'GTG
Cuts at: 221 1430 3072 221
Size: 1209 1642 3484
Fragments arranged by size:
3484 1642 1209
DrdI GACnn_nn'nnGTC
Cuts at: 4041 4899 4041
Size: 858 5477
EaeI y'GGCC_r
Cuts at: 225 308 393 477 535 600 731 827 921
Size: 83 85 84 58 65 131 96 94
Cuts at: 921 925 2006 2798 3920 4094 4485 4512 5857
Size: 4 1081 792 1122 174 391 27 1345
Cuts at: 5857 225
Size: 703
Fragments arranged by size:
1345 1122 1081 792 703 391 174 131
96 94 85 84 83 65 58 27
4
EagI C'GGCC_G
Cuts at: 393 925 2006 2798 3920 393
Size: 532 1081 792 1122 2808
Fragments arranged by size:
2808 1122 1081 792 532
EarI CTCTTCn'nnn_
Cuts at: 1565 1589 2089 2189 2975 3202 4358 4568 1565
Size: 24 500 100 786 227 1156 210 3332
Fragments arranged by size:
3332 1156 786 500 227 210 100 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1857 2488 4543 4853 4999 6145 1857
Size: 631 2055 310 146 1146 2047
Fragments arranged by size:
2055 2047 1146 631 310 146
EcoICRI GAG'CTC
Cuts at: 318 1874 2070 2079 2343 2361 2855 3347 318
Size: 1556 196 9 264 18 494 492 3306
Fragments arranged by size:
3306 1556 494 492 264 196 18 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 220 628 1390 1396 1624 1729 1968 2095
Size: 27 408 762 6 228 105 239 127
Cuts at: 2095 2200 2236 2458 2623 2989 3063 4160 4592
Size: 105 36 222 165 366 74 1097 432
Cuts at: 4592 5339 5607 193
Size: 747 268 921
Fragments arranged by size:
1097 921 762 747 432 408 366 268
239 228 222 165 127 105 105 74
36 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3073 3073
Size: 6335
EcoO109I rG'GnC_Cy
Cuts at: 195 204 2847 3474 195
Size: 9 2643 627 3056
Fragments arranged by size:
3056 2643 627 9
EcoRI G'AATT_C
Cuts at: 3339 3339
Size: 6335
EcoRV GAT'ATC
Cuts at: 2352 2352
Size: 6335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3240 4354 3240
Size: 1114 5221
FseI GG_CCGG'CC
Cuts at: 927 927
Size: 6335
FspI TGC'GCA
Cuts at: 2203 2332 4116 2203
Size: 129 1784 4422
Fragments arranged by size:
4422 1784 129
HaeII r_GCGC'y
Cuts at: 345 438 622 676 691 2061 2102 2396 2699
Size: 93 184 54 15 1370 41 294 303
Cuts at: 2699 3039 3769 4017 5039 6018 345
Size: 340 730 248 1022 979 662
Fragments arranged by size:
1370 1022 979 730 662 340 303 294
248 184 93 54 41 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 880 912 937 969 1233 1265 1858 1890 2327
Size: 32 25 32 264 32 593 32 437
Cuts at: 2327 2359 2839 2871 4177 4207 4209 4239 880
Size: 32 480 32 1306 30 2 30 2976
Fragments arranged by size:
2976 1306 593 480 437 264 32 32
32 32 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 987 1047 3374 987
Size: 60 2327 3948
Fragments arranged by size:
3948 2327 60
HindIII A'AGCT_T
Cuts at: 1091 1091
Size: 6335
KasI G'GCGC_C
Cuts at: 3765 4013 3765
Size: 248 6087
KpnI G_GTAC'C
Cuts at: 3355 3355
Size: 6335
MluI A'CGCG_T
Cuts at: 521 5768 521
Size: 5247 1088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1835 1940 2399 2849 3104 5006 5190 1835
Size: 105 459 450 255 1902 184 2980
Fragments arranged by size:
2980 1902 459 450 255 184 105
MscI TGG'CCA
Cuts at: 4096 4096
Size: 6335
MslI CAynn'nnrTG
Cuts at: 1120 1240 1702 2326 4324 4451 1120
Size: 120 462 624 1998 127 3004
Fragments arranged by size:
3004 1998 624 462 127 120
MspA1I CmG'CkG
Cuts at: 210 278 296 417 548 729 736 821 1182
Size: 68 18 121 131 181 7 85 361
Cuts at: 1182 1644 1832 1923 1970 2297 2486 2781 2990
Size: 462 188 91 47 327 189 295 209
Cuts at: 2990 3090 3446 3760 4120 5133 5378 210
Size: 100 356 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 462 361 360 356 327 314
295 245 209 189 188 181 131 121
100 91 85 68 47 18 7
NaeI GCC'GGC
Cuts at: 243 925 2145 4516 243
Size: 682 1220 2371 2062
Fragments arranged by size:
2371 2062 1220 682
NarI GG'CG_CC
Cuts at: 3766 4014 3766
Size: 248 6087
NcoI C'CATG_G
Cuts at: 125 125
Size: 6335
NgoMIV G'CCGG_C
Cuts at: 241 923 2143 4514 241
Size: 682 1220 2371 2062
Fragments arranged by size:
2371 2062 1220 682
NheI G'CTAG_C
Cuts at: 1744 1744
Size: 6335
NspI r_CATG'y
Cuts at: 516 1707 2778 2841 3388 3888 4795 516
Size: 1191 1071 63 547 500 907 2056
Fragments arranged by size:
2056 1191 1071 907 547 500 63
PasI CC'CwG_GG
Cuts at: 257 900 257
Size: 643 5692
PciI A'CATG_T
Cuts at: 4791 4791
Size: 6335
PflMI CCAn_nnn'nTGG
Cuts at: 1086 2628 2688 1086
Size: 1542 60 4733
Fragments arranged by size:
4733 1542 60
PfoI T'CCnGG_A
Cuts at: 197 2072 2278 2363 2975 197
Size: 1875 206 85 612 3557
Fragments arranged by size:
3557 1875 612 206 85
PmeI GTTT'AAAC
Cuts at: 3334 3334
Size: 6335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 5474
Size: 32 6303
PpuMI rG'GwC_Cy
Cuts at: 195 204 2847 195
Size: 9 2643 3683
Fragments arranged by size:
3683 2643 9
PshAI GACnn'nnGTC
Cuts at: 874 874
Size: 6335
PspXI vC'TCGA_Gb
Cuts at: 430 2388 430
Size: 1958 4377
PstI C_TGCA'G
Cuts at: 413 755 1002 1757 1951 3382 4067 413
Size: 342 247 755 194 1431 685 2681
Fragments arranged by size:
2681 1431 755 685 342 247 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6335
PvuII CAG'CTG
Cuts at: 278 417 729 1182 1644 2486 2781 3760 4120
Size: 139 312 453 462 842 295 979 360
Cuts at: 4120 278
Size: 2493
Fragments arranged by size:
2493 979 842 462 453 360 312 295
139
RsrII CG'GwC_CG
Cuts at: 1844 4530 1844
Size: 2686 3649
SacI G_AGCT'C
Cuts at: 320 1876 2072 2081 2345 2363 2857 3349 320
Size: 1556 196 9 264 18 494 492 3306
Fragments arranged by size:
3306 1556 494 492 264 196 18 9
SacII CC_GC'GG
Cuts at: 297 737 297
Size: 440 5895
SalI G'TCGA_C
Cuts at: 3372 3372
Size: 6335
SapI GCTCTTCn'nnn_
Cuts at: 1589 2089 2189 2975 4358 4568 1589
Size: 500 100 786 1383 210 3356
Fragments arranged by size:
3356 1383 786 500 210 100
SbfI CC_TGCA'GG
Cuts at: 3382 3382
Size: 6335
ScaI AGT'ACT
Cuts at: 5852 5852
Size: 6335
SexAI A'CCwGG_T
Cuts at: 3068 3068
Size: 6335
SfcI C'TryA_G
Cuts at: 33 409 751 998 1392 1753 1947 2589 3378
Size: 376 342 247 394 361 194 642 789
Cuts at: 3378 4063 5056 5247 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 789 685 642 394 376 361
342 247 194 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 730 730
Size: 6335
SfoI GGC'GCC
Cuts at: 3767 4015 3767
Size: 248 6087
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
SmaI CCC'GGG
Cuts at: 3357 3357
Size: 6335
SmlI C'TyrA_G
Cuts at: 337 352 430 676 745 2080 2257 2388 2460
Size: 15 78 246 69 1335 177 131 72
Cuts at: 2460 3133 3490 4405 4897 5159 337
Size: 673 357 915 492 262 1513
Fragments arranged by size:
1513 1335 915 673 492 357 262 246
177 131 78 72 69 15
SnaBI TAC'GTA
Cuts at: 3327 3327
Size: 6335
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6335
SphI G_CATG'C
Cuts at: 3388 3888 3388
Size: 500 5835
SspI AAT'ATT
Cuts at: 912 912
Size: 6335
StuI AGG'CCT
Cuts at: 1016 1632 1016
Size: 616 5719
StyI C'CwwG_G
Cuts at: 125 1004 1106 1144 1502 1961 3080 3469 3823
Size: 879 102 38 358 459 1119 389 354
Cuts at: 3823 125
Size: 2637
Fragments arranged by size:
2637 1119 879 459 389 358 354 102
38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 1861 3918 4051 4510 4719 249
Size: 1612 2057 133 459 209 1865
Fragments arranged by size:
2057 1865 1612 459 209 133
TatI w'GTAC_w
Cuts at: 2117 5850 2117
Size: 3733 2602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 352 384 2068 2100 2550 2582 5852
Size: 32 160 32 1684 32 450 32 3270
Cuts at: 5852 5884 160
Size: 32 611
Fragments arranged by size:
3270 1684 611 450 160 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4132 4132
Size: 6335
XbaI T'CTAG_A
Cuts at: 3366 3366
Size: 6335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2625 2685 3830 2625
Size: 60 1145 5130
Fragments arranged by size:
5130 1145 60
XhoI C'TCGA_G
Cuts at: 430 2388 430
Size: 1958 4377
XmaI C'CCGG_G
Cuts at: 3355 3355
Size: 6335
XmnI GAAnn'nnTTC
Cuts at: 781 3223 781
Size: 2442 3893
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BsmI BsmBI BsrGI BstEII Bsu36I
ClaI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PmlI PsiI PspOMI PsrI SanDI SgrAI SrfI
SwaI ZraI