Restriction map for
pF1KSDA1098
(Circular) (Six-base) MAPSORT of: pF1KSDA1098.seq Check: 4698 from: 1 to: 4618
pF1KSDA1098 4618 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 270 301 1110 270
Size: 31 809 3778
Fragments arranged by size:
3778 809 31
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4618
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4618
AclI AA'CG_TT
Cuts at: 4458 4458
Size: 4618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1020 2441 2873 3622 3890 1020
Size: 1421 432 749 268 1748
Fragments arranged by size:
1748 1421 749 432 268
AflIII A'CryG_T
Cuts at: 143 1245 1968 3074 4051 143
Size: 1102 723 1106 977 710
Fragments arranged by size:
1106 1102 977 723 710
AgeI A'CCGG_T
Cuts at: 3006 3006
Size: 4618
AhdI GACnn_n'nnGTC
Cuts at: 315 315
Size: 4618
AleI CACnn'nnGTG
Cuts at: 1580 1580
Size: 4618
AlwNI CAG_nnn'CTG
Cuts at: 1921 3490 1921
Size: 1569 3049
ApaLI G'TGCA_C
Cuts at: 1529 3388 1529
Size: 1859 2759
ApoI r'AATT_y
Cuts at: 1620 3971 4227 1620
Size: 2351 256 2011
Fragments arranged by size:
2351 2011 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4618
AvaI C'yCGr_G
Cuts at: 151 463 1260 1305 1636 151
Size: 312 797 45 331 3133
Fragments arranged by size:
3133 797 331 312 45
BamHI G'GATC_C
Cuts at: 683 1641 683
Size: 958 3660
BanI G'GyrC_C
Cuts at: 518 1149 1537 1632 2046 2294 2329 518
Size: 631 388 95 414 248 35 2807
Fragments arranged by size:
2807 631 414 388 248 95 35
BanII G_rGCy'C
Cuts at: 982 1168 1189 1630 2660 982
Size: 186 21 441 1030 2940
Fragments arranged by size:
2940 1030 441 186 21
BbeI G_GCGC'C
Cuts at: 1153 1541 2050 2298 1153
Size: 388 509 248 3473
Fragments arranged by size:
3473 509 388 248
BbsI GAAGACnn'nnnn_
Cuts at: 1167 1167
Size: 4618
BbvCI CC'TCA_GC
Cuts at: 1268 1268
Size: 4618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 933 939 973 899
Size: 34 6 34 4544
Fragments arranged by size:
4544 34 34 6
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3277 4618 2514
Size: 763 1341 2514
Fragments arranged by size:
2514 1341 763
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4618
BglI GCCn_nnn'nGGC
Cuts at: 613 1163 613
Size: 550 4068
BglII A'GATC_T
Cuts at: 2131 4170 2131
Size: 2039 2579
BlpI GC'TnA_GC
Cuts at: 448 1728 448
Size: 1280 3338
Bme1580I G_kGCm'C
Cuts at: 521 1533 2241 2334 3392 521
Size: 1012 708 93 1058 1747
Fragments arranged by size:
1747 1058 1012 708 93
BmgBI CAC'GTC
Cuts at: 144 1376 144
Size: 1232 3386
BmrI ACTGGGnnnn_n'
Cuts at: 292 1828 1909 2244 4390 292
Size: 1536 81 335 2146 520
Fragments arranged by size:
2146 1536 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1293 2983 1293
Size: 1690 2928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1248 1441 1532 1248
Size: 193 91 4334
Fragments arranged by size:
4334 193 91
Bpu10I CC'TnA_GC
Cuts at: 1268 1268
Size: 4618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 738 1792 2671 3165 3463 738
Size: 1054 879 494 298 1893
Fragments arranged by size:
1893 1054 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4322 4322
Size: 4618
BsaAI yAC'GTr
Cuts at: 1246 1608 1969 1246
Size: 362 361 3895
Fragments arranged by size:
3895 362 361
BsaBI GATnn'nnATC
Cuts at: 6 2130 4169 6
Size: 2124 2039 455
Fragments arranged by size:
2124 2039 455
BsaHI Gr'CG_yC
Cuts at: 1150 1538 2047 2295 2999 4495 1150
Size: 388 509 248 704 1496 1273
Fragments arranged by size:
1496 1273 704 509 388 248
BsaWI w'CCGG_w
Cuts at: 1805 2326 3006 3280 3427 3838 1805
Size: 521 680 274 147 411 2585
Fragments arranged by size:
2585 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 964 994 1408 1438 466
Size: 30 468 30 414 30 3646
Fragments arranged by size:
3646 468 414 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 394 742 772 826 1134 1444 190
Size: 204 348 30 54 308 310 3364
Fragments arranged by size:
3364 348 310 308 204 54 30
BseYI C'CCAG_C
Cuts at: 269 1161 1368 2041 2799 3378 3874 269
Size: 892 207 673 758 579 496 1013
Fragments arranged by size:
1013 892 758 673 579 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 418 547 588 903 1138 298
Size: 120 129 41 315 235 3778
Fragments arranged by size:
3778 315 235 129 120 41
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3414 104
Size: 2054 46 1210 1308
Fragments arranged by size:
2054 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1533 1630 2408 2598 3392 1533
Size: 97 778 190 794 2759
Fragments arranged by size:
2759 794 778 190 97
BsmBI CGTCTCn'nnnn_
Cuts at: 808 808
Size: 4618
Bsp1286I G_dGCh'C
Cuts at: 521 982 1168 1189 1533 1630 2241 2334 2408
Size: 461 186 21 344 97 611 93 74
Cuts at: 2408 2598 2660 3392 521
Size: 190 62 732 1747
Fragments arranged by size:
1747 732 611 461 344 190 186 97
93 74 62 21
BspEI T'CCGG_A
Cuts at: 3838 3838
Size: 4618
BspHI T'CATG_A
Cuts at: 884 3794 884
Size: 2910 1708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 270 301 338 1110 1666 1880 2182 2563 270
Size: 31 37 772 556 214 302 381 2325
Fragments arranged by size:
2325 772 556 381 302 214 37 31
BsrBI CCG'CTC
Cuts at: 50 132 164 843 2908 2962 4448 4613 50
Size: 82 32 679 2065 54 1486 165 55
Fragments arranged by size:
2065 1486 679 165 82 55 54 32
BsrDI GCAATG_nn'
Cuts at: 2528 2528
Size: 4618
BsrFI r'CCGG_y
Cuts at: 531 2614 2795 3006 531
Size: 2083 181 211 2143
Fragments arranged by size:
2143 2083 211 181
BsrGI T'GTAC_A
Cuts at: 1475 1475
Size: 4618
BssHII G'CGCG_C
Cuts at: 392 394 392
Size: 2 4616
BssSI C'ACGA_G
Cuts at: 2887 3247 2887
Size: 360 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 525 588 1993 525
Size: 63 1405 3150
Fragments arranged by size:
3150 1405 63
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4618
BstYI r'GATC_y
Cuts at: 1 683 1641 2108 2131 2466 2712 3726 3812
Size: 682 958 467 23 335 246 1014 86
Cuts at: 3812 3824 4170 1
Size: 12 346 449
Fragments arranged by size:
1014 958 682 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 249 438 749 923 1104 125
Size: 124 189 311 174 181 3639
Fragments arranged by size:
3639 311 189 181 174 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 624 2566 2747 2910 624
Size: 1942 181 163 2332
Fragments arranged by size:
2332 1942 181 163
BtsI GCAGTG_nn'
Cuts at: 542 586 1350 1464 1859 542
Size: 44 764 114 395 3301
Fragments arranged by size:
3301 764 395 114 44
ClaI AT'CG_AT
Cuts at: 959 959
Size: 4618
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4618
DraIII CAC_nnn'GTG
Cuts at: 525 1356 525
Size: 831 3787
DrdI GACnn_nn'nnGTC
Cuts at: 2322 3182 2322
Size: 860 3758
EaeI y'GGCC_r
Cuts at: 234 752 782 1391 2201 2375 2766 2793 4140
Size: 518 30 609 810 174 391 27 1347
Cuts at: 4140 234
Size: 712
Fragments arranged by size:
1347 810 712 609 518 391 174 30
27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4618
EarI CTCTTCn'nnn_
Cuts at: 460 919 2639 2849 460
Size: 459 1720 210 2229
Fragments arranged by size:
2229 1720 459 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3136 3282 4428 2824
Size: 312 146 1146 3014
Fragments arranged by size:
3014 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1628 1628
Size: 4618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1020 1248 1441 1532 2441 2873 3622 3890 1020
Size: 228 193 91 909 432 749 268 1748
Fragments arranged by size:
1748 909 749 432 268 228 193 91
EcoO109I rG'GnC_Cy
Cuts at: 1755 1755
Size: 4618
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4618
FspI TGC'GCA
Cuts at: 334 1561 2397 334
Size: 1227 836 2555
Fragments arranged by size:
2555 1227 836
HaeII r_GCGC'y
Cuts at: 627 1153 1268 1464 1541 2050 2298 3322 4301
Size: 526 115 196 77 509 248 1024 979
Cuts at: 4301 627
Size: 944
Fragments arranged by size:
1024 979 944 526 509 248 196 115
77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 1288 1320 2458 2488 2490 2520 301
Size: 32 955 32 1138 30 2 30 2399
Fragments arranged by size:
2399 1138 955 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1655 1655
Size: 4618
HindIII A'AGCT_T
Cuts at: 703 703
Size: 4618
KasI G'GCGC_C
Cuts at: 1149 1537 2046 2294 1149
Size: 388 509 248 3473
Fragments arranged by size:
3473 509 388 248
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4618
MluI A'CGCG_T
Cuts at: 4051 4051
Size: 4618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3289 3473 3289
Size: 184 4434
MscI TGG'CCA
Cuts at: 1393 2377 1393
Size: 984 3634
MslI CAynn'nnrTG
Cuts at: 1580 2605 2732 1580
Size: 1025 127 3466
Fragments arranged by size:
3466 1025 127
MspA1I CmG'CkG
Cuts at: 251 266 440 1060 1286 1727 2041 2401 3416
Size: 15 174 620 226 441 314 360 1015
Cuts at: 3416 3661 251
Size: 245 1208
Fragments arranged by size:
1208 1015 620 441 360 314 245 226
174 15
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4618
NarI GG'CG_CC
Cuts at: 1150 1538 2047 2295 1150
Size: 388 509 248 3473
Fragments arranged by size:
3473 509 388 248
NcoI C'CATG_G
Cuts at: 125 749 923 1104 125
Size: 624 174 181 3639
Fragments arranged by size:
3639 624 181 174
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4618
NruI TCG'CGA
Cuts at: 710 2979 710
Size: 2269 2349
NspI r_CATG'y
Cuts at: 586 954 1669 2169 3078 586
Size: 368 715 500 909 2126
Fragments arranged by size:
2126 909 715 500 368
PciI A'CATG_T
Cuts at: 3074 3074
Size: 4618
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3789 3757
Size: 32 4586
PspXI vC'TCGA_Gb
Cuts at: 463 463
Size: 4618
PstI C_TGCA'G
Cuts at: 851 989 1223 1663 2348 851
Size: 138 234 440 685 3121
Fragments arranged by size:
3121 685 440 234 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4618
PvuII CAG'CTG
Cuts at: 1060 1286 2041 2401 1060
Size: 226 755 360 3277
Fragments arranged by size:
3277 755 360 226
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4618
SacI G_AGCT'C
Cuts at: 1630 1630
Size: 4618
SacII CC_GC'GG
Cuts at: 252 441 252
Size: 189 4429
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4618
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4408
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4618
ScaI AGT'ACT
Cuts at: 4135 4135
Size: 4618
SexAI A'CCwGG_T
Cuts at: 356 356
Size: 4618
SfcI C'TryA_G
Cuts at: 33 847 985 1219 1659 2344 3339 3530 33
Size: 814 138 234 440 685 995 191 1121
Fragments arranged by size:
1121 995 814 685 440 234 191 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 613 613
Size: 4618
SfoI GGC'GCC
Cuts at: 1151 1539 2048 2296 1151
Size: 388 509 248 3473
Fragments arranged by size:
3473 509 388 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4618
SmaI CCC'GGG
Cuts at: 153 1638 153
Size: 1485 3133
SmlI C'TyrA_G
Cuts at: 463 717 1771 2686 3180 3442 463
Size: 254 1054 915 494 262 1639
Fragments arranged by size:
1639 1054 915 494 262 254
SnaBI TAC'GTA
Cuts at: 1608 1608
Size: 4618
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4618
SphI G_CATG'C
Cuts at: 586 954 1669 2169 586
Size: 368 715 500 3035
Fragments arranged by size:
3035 715 500 368
StuI AGG'CCT
Cuts at: 607 607
Size: 4618
StyI C'CwwG_G
Cuts at: 125 602 749 923 1104 1209 1750 2104 125
Size: 477 147 174 181 105 541 354 2639
Fragments arranged by size:
2639 541 477 354 181 174 147 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2199 2332 2791 3002 2199
Size: 133 459 211 3815
Fragments arranged by size:
3815 459 211 133
TatI w'GTAC_w
Cuts at: 1475 4133 1475
Size: 2658 1960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 1408 1440 4135 4167 466
Size: 32 910 32 2695 32 917
Fragments arranged by size:
2695 917 910 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 217 2413 217
Size: 2196 2422
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1380 1492 2111 1380
Size: 112 619 3887
Fragments arranged by size:
3887 619 112
XhoI C'TCGA_G
Cuts at: 463 463
Size: 4618
XmaI C'CCGG_G
Cuts at: 151 1636 151
Size: 1485 3133
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BstEII BstXI BstZ17I
Bsu36I CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PsrI SanDI SgrAI
SrfI SspI SwaI XmnI ZraI