Restriction map for
pF1KSDA1099
(Circular) (Six-base) MAPSORT of: pF1KSDA1099.seq Check: 9527 from: 1 to: 5551
pF1KSDA1099 5551 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 891 2180 891
Size: 1289 4262
AatII G_ACGT'C
Cuts at: 2351 2351
Size: 5551
AccI GT'mk_AC
Cuts at: 2069 2587 2069
Size: 518 5033
Acc65I G'GTAC_C
Cuts at: 395 2565 395
Size: 2170 3381
AclI AA'CG_TT
Cuts at: 5391 5391
Size: 5551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1628 3374 3806 4555 4823 540
Size: 1088 1746 432 749 268 1268
Fragments arranged by size:
1746 1268 1088 749 432 268
AfeI AGC'GCT
Cuts at: 181 2455 181
Size: 2274 3277
AflIII A'CryG_T
Cuts at: 754 2901 4007 4984 754
Size: 2147 1106 977 1321
Fragments arranged by size:
2147 1321 1106 977
AgeI A'CCGG_T
Cuts at: 392 3939 392
Size: 3547 2004
AhdI GACnn_n'nnGTC
Cuts at: 320 1593 320
Size: 1273 4278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 923 2299 2440 923
Size: 1376 141 4034
Fragments arranged by size:
4034 1376 141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 2084
Size: 32 5519
AlwNI CAG_nnn'CTG
Cuts at: 144 837 1728 2030 2172 2328 2854 4423 144
Size: 693 891 302 142 156 526 1569 1272
Fragments arranged by size:
1569 1272 891 693 526 302 156 142
ApaI G_GGCC'C
Cuts at: 508 508
Size: 5551
ApaLI G'TGCA_C
Cuts at: 388 738 913 4321 388
Size: 350 175 3408 1618
Fragments arranged by size:
3408 1618 350 175
ApoI r'AATT_y
Cuts at: 1623 2553 4904 5160 1623
Size: 930 2351 256 2014
Fragments arranged by size:
2351 2014 930 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5551
AvaI C'yCGr_G
Cuts at: 684 1358 1943 2234 2357 2569 684
Size: 674 585 291 123 212 3666
Fragments arranged by size:
3666 674 585 291 212 123
BamHI G'GATC_C
Cuts at: 1913 2099 2574 1913
Size: 186 475 4890
Fragments arranged by size:
4890 475 186
BanI G'GyrC_C
Cuts at: 395 1930 2523 2565 2979 3227 3262 395
Size: 1535 593 42 414 248 35 2684
Fragments arranged by size:
2684 1535 593 414 248 42 35
BanII G_rGCy'C
Cuts at: 341 508 1890 1985 2563 3593 341
Size: 167 1382 95 578 1030 2299
Fragments arranged by size:
2299 1382 1030 578 167 95
BbeI G_GCGC'C
Cuts at: 2983 3231 2983
Size: 248 5303
BbsI GAAGACnn'nnnn_
Cuts at: 546 780 546
Size: 234 5317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 773 739
Size: 34 5517
BciVI GTATCCnnnnn_n'
Cuts at: 3447 4210 5551 3447
Size: 763 1341 3447
Fragments arranged by size:
3447 1341 763
BclI T'GATC_A
Cuts at: 488 3069 488
Size: 2581 2970
BglI GCCn_nnn'nGGC
Cuts at: 154 166 1598 2398 154
Size: 12 1432 800 3307
Fragments arranged by size:
3307 1432 800 12
BglII A'GATC_T
Cuts at: 3064 5103 3064
Size: 2039 3512
BlpI GC'TnA_GC
Cuts at: 2661 2661
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 392 409 508 742 917 2528 3174 3267 4325
Size: 17 99 234 175 1611 646 93 1058
Cuts at: 4325 392
Size: 1618
Fragments arranged by size:
1618 1611 1058 646 234 175 99 93
17
BmgBI CAC'GTC
Cuts at: 273 755 1367 2424 273
Size: 482 612 1057 3400
Fragments arranged by size:
3400 1057 612 482
BmrI ACTGGGnnnn_n'
Cuts at: 1265 1478 1875 2761 2842 3177 5323 1265
Size: 213 397 886 81 335 2146 1493
Fragments arranged by size:
2146 1493 886 397 335 213 81
BmtI G_CTAG'C
Cuts at: 2020 2020
Size: 5551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1144 1371 1461 1476 2388 247
Size: 897 227 90 15 912 3410
Fragments arranged by size:
3410 912 897 227 90 15
Bpu10I CC'TnA_GC
Cuts at: 1908 1908
Size: 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2725 3604 4098 4396 2725
Size: 879 494 298 3880
Fragments arranged by size:
3880 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1843 2245 5255 1843
Size: 402 3010 2139
Fragments arranged by size:
3010 2139 402
BsaAI yAC'GTr
Cuts at: 743 2541 2902 743
Size: 1798 361 3392
Fragments arranged by size:
3392 1798 361
BsaBI GATnn'nnATC
Cuts at: 6 3063 5102 6
Size: 3057 2039 455
Fragments arranged by size:
3057 2039 455
BsaHI Gr'CG_yC
Cuts at: 701 1697 2348 2980 3228 3932 5428 701
Size: 996 651 632 248 704 1496 824
Fragments arranged by size:
1496 996 824 704 651 632 248
BsaWI w'CCGG_w
Cuts at: 392 429 1811 1919 2501 2738 3259 3939 4213
Size: 37 1382 108 582 237 521 680 274
Cuts at: 4213 4360 4771 392
Size: 147 411 1172
Fragments arranged by size:
1382 1172 680 582 521 411 274 237
147 108 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 505 535 946 976 2333 2363 193
Size: 30 282 30 411 30 1357 30 3381
Fragments arranged by size:
3381 1357 411 282 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 436 954 1702 253
Size: 183 518 748 4102
Fragments arranged by size:
4102 748 518 183
BseYI C'CCAG_C
Cuts at: 874 982 1458 2974 3732 4311 4807 874
Size: 108 476 1516 758 579 496 1618
Fragments arranged by size:
1618 1516 758 579 496 476 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2130 2130
Size: 5551
BsiEI CG_ry'CG
Cuts at: 104 1155 2274 3091 3137 4347 104
Size: 1051 1119 817 46 1210 1308
Fragments arranged by size:
1308 1210 1119 1051 817 46
BsiHKAI G_wGCw'C
Cuts at: 272 341 392 742 917 1985 2465 2563 3341
Size: 69 51 350 175 1068 480 98 778
Cuts at: 3341 3531 4325 272
Size: 190 794 1498
Fragments arranged by size:
1498 1068 794 778 480 350 190 175
98 69 51
BsmI GAATG_Cn'
Cuts at: 1909 1909
Size: 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 745 2261 745
Size: 1516 4035
Bsp1286I G_dGCh'C
Cuts at: 272 341 392 409 508 742 917 1890 1985
Size: 69 51 17 99 234 175 973 95
Cuts at: 1985 2465 2528 2563 3174 3267 3341 3531 3593
Size: 480 63 35 611 93 74 190 62
Cuts at: 3593 4325 272
Size: 732 1498
Fragments arranged by size:
1498 973 732 611 480 234 190 175
99 95 93 74 69 63 62 51
35 17
BspEI T'CCGG_A
Cuts at: 429 1811 4771 429
Size: 1382 2960 1209
Fragments arranged by size:
2960 1382 1209
BspHI T'CATG_A
Cuts at: 4727 4727
Size: 5551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 891 2180 2207 2263 2599 2813 3115 3496
Size: 460 1289 27 56 336 214 302 381
Cuts at: 3496 431
Size: 2486
Fragments arranged by size:
2486 1289 460 381 336 302 214 56
27
BsrBI CCG'CTC
Cuts at: 50 1151 1692 3841 3895 5381 5546 50
Size: 1101 541 2149 54 1486 165 55
Fragments arranged by size:
2149 1486 1101 541 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3461 3461
Size: 5551
BsrFI r'CCGG_y
Cuts at: 243 392 1064 3547 3728 3939 243
Size: 149 672 2483 181 211 1855
Fragments arranged by size:
2483 1855 672 211 181 149
BssSI C'ACGA_G
Cuts at: 627 3820 4180 627
Size: 3193 360 1998
Fragments arranged by size:
3193 1998 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1904 2175 2926 1904
Size: 271 751 4529
Fragments arranged by size:
4529 751 271
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5551
BstXI CCAn_nnnn'nTGG
Cuts at: 534 534
Size: 5551
BstYI r'GATC_y
Cuts at: 1 173 260 1724 1913 2099 2574 3041 3064
Size: 172 87 1464 189 186 475 467 23
Cuts at: 3064 3399 3645 4659 4745 4757 5103 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1464 1014 475 467 449 346 335 246
189 186 172 87 86 23 12
BtgI C'CryG_G
Cuts at: 1793 2367 1793
Size: 574 4977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 261 3499 3680 3843 261
Size: 3238 181 163 1969
Fragments arranged by size:
3238 1969 181 163
BtsI GCAGTG_nn'
Cuts at: 1022 1601 2520 2792 1022
Size: 579 919 272 3781
Fragments arranged by size:
3781 919 579 272
ClaI AT'CG_AT
Cuts at: 695 1010 695
Size: 315 5236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2496 2531 2496
Size: 35 5516
DraI TTT'AAA
Cuts at: 2548 2548
Size: 5551
DraIII CAC_nnn'GTG
Cuts at: 1241 1839 1241
Size: 598 4953
DrdI GACnn_nn'nnGTC
Cuts at: 1702 3255 4115 1702
Size: 1553 860 3138
Fragments arranged by size:
3138 1553 860
EaeI y'GGCC_r
Cuts at: 146 365 611 1152 1653 1730 1972 2207 2271
Size: 219 246 541 501 77 242 235 64
Cuts at: 2271 2468 3134 3308 3699 3726 5073 146
Size: 197 666 174 391 27 1347 624
Fragments arranged by size:
1347 666 624 541 501 391 246 242
235 219 197 174 77 64 27
EagI C'GGCC_G
Cuts at: 1152 2271 3134 1152
Size: 1119 863 3569
Fragments arranged by size:
3569 1119 863
EarI CTCTTCn'nnn_
Cuts at: 1342 2030 2078 2131 3572 3782 1342
Size: 688 48 53 1441 210 3111
Fragments arranged by size:
3111 1441 688 210 53 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2067 3757 4069 4215 5361 2067
Size: 1690 312 146 1146 2257
Fragments arranged by size:
2257 1690 1146 312 146
EcoICRI GAG'CTC
Cuts at: 339 1983 2561 339
Size: 1644 578 3329
Fragments arranged by size:
3329 1644 578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 540 1144 1371 1461 1476 1628 2388 3374
Size: 293 604 227 90 15 152 760 986
Cuts at: 3374 3806 4555 4823 247
Size: 432 749 268 975
Fragments arranged by size:
986 975 760 749 604 432 293 268
227 152 90 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1411 1411
Size: 5551
EcoO109I rG'GnC_Cy
Cuts at: 504 505 847 2196 2688 504
Size: 1 342 1349 492 3367
Fragments arranged by size:
3367 1349 492 342 1
EcoRI G'AATT_C
Cuts at: 2553 2553
Size: 5551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3568 3568
Size: 5551
FspI TGC'GCA
Cuts at: 1377 3330 1377
Size: 1953 3598
HaeII r_GCGC'y
Cuts at: 183 1065 2284 2457 2983 3231 4255 5234 183
Size: 882 1219 173 526 248 1024 979 500
Fragments arranged by size:
1219 1024 979 882 526 500 248 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 306 338 946 978 1251 1283 1579 1611 1842
Size: 32 608 32 273 32 296 32 231
Cuts at: 1842 1874 2333 2365 3391 3421 3423 3453 306
Size: 32 459 32 1026 30 2 30 2404
Fragments arranged by size:
2404 1026 608 459 296 273 231 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2588 2588
Size: 5551
KasI G'GCGC_C
Cuts at: 2979 3227 2979
Size: 248 5303
KpnI G_GTAC'C
Cuts at: 399 2569 399
Size: 2170 3381
MluI A'CGCG_T
Cuts at: 4984 4984
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 746 1094 4222 4406 746
Size: 348 3128 184 1891
Fragments arranged by size:
3128 1891 348 184
MscI TGG'CCA
Cuts at: 148 367 613 1655 1732 1974 2470 3310 148
Size: 219 246 1042 77 242 496 840 2389
Fragments arranged by size:
2389 1042 840 496 246 242 219 77
MslI CAynn'nnrTG
Cuts at: 532 1990 2422 3538 3665 532
Size: 1458 432 1116 127 2418
Fragments arranged by size:
2418 1458 1116 432 127
MspA1I CmG'CkG
Cuts at: 144 371 837 2139 2660 2974 3334 4349 4594
Size: 227 466 1302 521 314 360 1015 245
Cuts at: 4594 144
Size: 1101
Fragments arranged by size:
1302 1101 1015 521 466 360 314 245
227
NaeI GCC'GGC
Cuts at: 3730 3730
Size: 5551
NarI GG'CG_CC
Cuts at: 2980 3228 2980
Size: 248 5303
NcoI C'CATG_G
Cuts at: 1793 1793
Size: 5551
NdeI CA'TA_TG
Cuts at: 411 411
Size: 5551
NgoMIV G'CCGG_C
Cuts at: 3728 3728
Size: 5551
NheI G'CTAG_C
Cuts at: 2016 2016
Size: 5551
NruI TCG'CGA
Cuts at: 3912 3912
Size: 5551
NspI r_CATG'y
Cuts at: 1182 1290 1821 2602 3102 4011 1182
Size: 108 531 781 500 909 2722
Fragments arranged by size:
2722 909 781 531 500 108
PasI CC'CwG_GG
Cuts at: 1342 2160 1342
Size: 818 4733
PciI A'CATG_T
Cuts at: 4007 4007
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1728 1839 1728
Size: 111 5440
PfoI T'CCnGG_A
Cuts at: 263 332 416 791 2485 263
Size: 69 84 375 1694 3329
Fragments arranged by size:
3329 1694 375 84 69
PmeI GTTT'AAAC
Cuts at: 2548 2548
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4690 4722 4690
Size: 32 5519
PpuMI rG'GwC_Cy
Cuts at: 847 2196 847
Size: 1349 4202
PspOMI G'GGCC_C
Cuts at: 504 504
Size: 5551
PstI C_TGCA'G
Cuts at: 254 1742 1805 2435 2596 3281 254
Size: 1488 63 630 161 685 2524
Fragments arranged by size:
2524 1488 685 630 161 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5551
PvuII CAG'CTG
Cuts at: 144 837 2974 3334 144
Size: 693 2137 360 2361
Fragments arranged by size:
2361 2137 693 360
RsrII CG'GwC_CG
Cuts at: 3744 3744
Size: 5551
SacI G_AGCT'C
Cuts at: 341 1985 2563 341
Size: 1644 578 3329
Fragments arranged by size:
3329 1644 578
SalI G'TCGA_C
Cuts at: 2586 2586
Size: 5551
SapI GCTCTTCn'nnn_
Cuts at: 2131 3572 3782 2131
Size: 1441 210 3900
Fragments arranged by size:
3900 1441 210
SbfI CC_TGCA'GG
Cuts at: 2596 2596
Size: 5551
ScaI AGT'ACT
Cuts at: 5068 5068
Size: 5551
SfcI C'TryA_G
Cuts at: 33 250 600 1738 1801 2431 2592 3277 4272
Size: 217 350 1138 63 630 161 685 995
Cuts at: 4272 4463 33
Size: 191 1121
Fragments arranged by size:
1138 1121 995 685 630 350 217 191
161 63
SfoI GGC'GCC
Cuts at: 2981 3229 2981
Size: 248 5303
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5551
SmaI CCC'GGG
Cuts at: 2236 2571 2236
Size: 335 5216
SmlI C'TyrA_G
Cuts at: 2704 3619 4113 4375 2704
Size: 915 494 262 3880
Fragments arranged by size:
3880 915 494 262
SnaBI TAC'GTA
Cuts at: 2541 2541
Size: 5551
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5551
SphI G_CATG'C
Cuts at: 1182 2602 3102 1182
Size: 1420 500 3631
Fragments arranged by size:
3631 1420 500
StuI AGG'CCT
Cuts at: 1121 2401 1121
Size: 1280 4271
StyI C'CwwG_G
Cuts at: 1412 1793 2683 3037 1412
Size: 381 890 354 3926
Fragments arranged by size:
3926 890 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 522 1395 1450 1455 1948 3132 3265 3724
Size: 342 873 55 5 493 1184 133 459
Cuts at: 3724 3935 180
Size: 211 1796
Fragments arranged by size:
1796 1184 873 493 459 342 211 133
55 5
TatI w'GTAC_w
Cuts at: 5066 5066
Size: 5551
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5068 5100 5068
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 3346 3346
Size: 5551
XbaI T'CTAG_A
Cuts at: 2580 2580
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 931 1878 1924 2217 3044 931
Size: 947 46 293 827 3438
Fragments arranged by size:
3438 947 827 293 46
XmaI C'CCGG_G
Cuts at: 2234 2569 2234
Size: 335 5216
XmnI GAAnn'nnTTC
Cuts at: 2125 2125
Size: 5551
ZraI GAC'GTC
Cuts at: 2349 2349
Size: 5551
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I EcoRV FseI FspAI
HindIII HpaI MfeI NotI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI