Restriction map for
pF1KSDA1112
(Circular) (Six-base) MAPSORT of: pF1KSDA1112.seq Check: 7193 from: 1 to: 5149
pF1KSDA1112 5149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 136 1040 1886 1935 136
Size: 904 846 49 3350
Fragments arranged by size:
3350 904 846 49
AatII G_ACGT'C
Cuts at: 785 785
Size: 5149
AccI GT'mk_AC
Cuts at: 2185 2185
Size: 5149
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5149
AclI AA'CG_TT
Cuts at: 4989 4989
Size: 5149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 2972 3404 4153 4421 743
Size: 2229 432 749 268 1471
Fragments arranged by size:
2229 1471 749 432 268
AfeI AGC'GCT
Cuts at: 1912 1912
Size: 5149
AflIII A'CryG_T
Cuts at: 1082 1319 1742 2499 3605 4582 1082
Size: 237 423 757 1106 977 1649
Fragments arranged by size:
1649 1106 977 757 423 237
AgeI A'CCGG_T
Cuts at: 3537 3537
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 571
Size: 5149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 1159
Size: 32 5117
AlwNI CAG_nnn'CTG
Cuts at: 262 550 762 2452 4021 262
Size: 288 212 1690 1569 1390
Fragments arranged by size:
1690 1569 1390 288 212
ApaI G_GGCC'C
Cuts at: 240 240
Size: 5149
ApaLI G'TGCA_C
Cuts at: 406 1884 3919 406
Size: 1478 2035 1636
Fragments arranged by size:
2035 1636 1478
ApoI r'AATT_y
Cuts at: 2151 4502 4758 2151
Size: 2351 256 2542
Fragments arranged by size:
2542 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5149
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
AvaI C'yCGr_G
Cuts at: 1632 1646 1897 2167 1632
Size: 14 251 270 4614
Fragments arranged by size:
4614 270 251 14
AvrII C'CTAG_G
Cuts at: 289 289
Size: 5149
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5149
BanI G'GyrC_C
Cuts at: 1210 1858 2163 2577 2825 2860 1210
Size: 648 305 414 248 35 3499
Fragments arranged by size:
3499 648 414 305 248 35
BanII G_rGCy'C
Cuts at: 240 570 695 896 1292 1576 1618 2161 3191
Size: 330 125 201 396 284 42 543 1030
Cuts at: 3191 240
Size: 2198
Fragments arranged by size:
2198 1030 543 396 330 284 201 125
42
BbeI G_GCGC'C
Cuts at: 1862 2581 2829 1862
Size: 719 248 4182
Fragments arranged by size:
4182 719 248
BbsI GAAGACnn'nnnn_
Cuts at: 349 349
Size: 5149
BbvCI CC'TCA_GC
Cuts at: 874 1003 874
Size: 129 5020
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1192 1226 1540 1574 1192
Size: 34 314 34 4767
Fragments arranged by size:
4767 314 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3808 5149 3045
Size: 763 1341 3045
Fragments arranged by size:
3045 1341 763
BclI T'GATC_A
Cuts at: 2667 2667
Size: 5149
BglI GCCn_nnn'nGGC
Cuts at: 916 916
Size: 5149
BglII A'GATC_T
Cuts at: 1244 1403 1599 2662 4701 1244
Size: 159 196 1063 2039 1692
Fragments arranged by size:
2039 1692 1063 196 159
BlpI GC'TnA_GC
Cuts at: 1309 2259 1309
Size: 950 4199
Bme1580I G_kGCm'C
Cuts at: 240 410 1888 2772 2865 3923 240
Size: 170 1478 884 93 1058 1466
Fragments arranged by size:
1478 1466 1058 884 170 93
BmgBI CAC'GTC
Cuts at: 378 1743 378
Size: 1365 3784
BmrI ACTGGGnnnn_n'
Cuts at: 152 1596 2359 2440 2775 4921 152
Size: 1444 763 81 335 2146 380
Fragments arranged by size:
2146 1444 763 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1618 3514 1618
Size: 1896 3253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 437 450 621 680 1186 1243 1801 1816
Size: 79 13 171 59 506 57 558 15
Cuts at: 1816 358
Size: 3691
Fragments arranged by size:
3691 558 506 171 79 59 57 15
13
Bpu10I CC'TnA_GC
Cuts at: 874 1003 1198 874
Size: 129 195 4825
Fragments arranged by size:
4825 195 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 1646 1903 2323 3202 3696 3994 1561
Size: 85 257 420 879 494 298 2716
Fragments arranged by size:
2716 879 494 420 298 257 85
BsaI GGTCTCn'nnnn_
Cuts at: 1808 4853 1808
Size: 3045 2104
BsaAI yAC'GTr
Cuts at: 1320 2139 2500 1320
Size: 819 361 3969
Fragments arranged by size:
3969 819 361
BsaBI GATnn'nnATC
Cuts at: 6 1363 2661 4700 6
Size: 1357 1298 2039 455
Fragments arranged by size:
2039 1357 1298 455
BsaHI Gr'CG_yC
Cuts at: 782 908 1859 2578 2826 3530 5026 782
Size: 126 951 719 248 704 1496 905
Fragments arranged by size:
1496 951 905 719 704 248 126
BsaWI w'CCGG_w
Cuts at: 1392 2336 2857 3537 3811 3958 4369 1392
Size: 944 521 680 274 147 411 2172
Fragments arranged by size:
2172 944 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 1159 1189 601
Size: 30 528 30 4561
Fragments arranged by size:
4561 528 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 622 1108 2060 232
Size: 390 486 952 3321
Fragments arranged by size:
3321 952 486 390
BseYI C'CCAG_C
Cuts at: 762 775 1206 1454 1511 2572 3330 3909 4405
Size: 13 431 248 57 1061 758 579 496
Cuts at: 4405 762
Size: 1506
Fragments arranged by size:
1506 1061 758 579 496 431 248 57
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 613 1417 1834 1969 376
Size: 237 804 417 135 3556
Fragments arranged by size:
3556 804 417 237 135
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3945 104
Size: 2585 46 1210 1308
Fragments arranged by size:
2585 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 410 504 539 570 695 1292 1576 1618 1888
Size: 94 35 31 125 597 284 42 270
Cuts at: 1888 2161 2939 3129 3923 410
Size: 273 778 190 794 1636
Fragments arranged by size:
1636 794 778 597 284 273 270 190
125 94 42 35 31
BsmI GAATG_Cn'
Cuts at: 2016 2016
Size: 5149
Bsp1286I G_dGCh'C
Cuts at: 240 410 504 539 570 695 896 1292 1576
Size: 170 94 35 31 125 201 396 284
Cuts at: 1576 1618 1888 2161 2772 2865 2939 3129 3191
Size: 42 270 273 611 93 74 190 62
Cuts at: 3191 3923 240
Size: 732 1466
Fragments arranged by size:
1466 732 611 396 284 273 270 201
190 170 125 94 93 74 62 42
35 31
BspEI T'CCGG_A
Cuts at: 4369 4369
Size: 5149
BspHI T'CATG_A
Cuts at: 4325 4325
Size: 5149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 635 1040 1736 1886 1935 2016 2197 2411
Size: 499 405 696 150 49 81 181 214
Cuts at: 2411 2713 3094 136
Size: 302 381 2191
Fragments arranged by size:
2191 696 499 405 381 302 214 181
150 81 49
BsrBI CCG'CTC
Cuts at: 50 308 934 1014 1527 3439 3493 4979 5144
Size: 258 626 80 513 1912 54 1486 165
Cuts at: 5144 50
Size: 55
Fragments arranged by size:
1912 1486 626 513 258 165 80 55
54
BsrDI GCAATG_nn'
Cuts at: 3059 3059
Size: 5149
BsrFI r'CCGG_y
Cuts at: 1340 1760 3145 3326 3537 1340
Size: 420 1385 181 211 2952
Fragments arranged by size:
2952 1385 420 211 181
BssHII G'CGCG_C
Cuts at: 1074 1074
Size: 5149
BssSI C'ACGA_G
Cuts at: 3418 3778 3418
Size: 360 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 2004 2524 2004
Size: 520 4629
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 5149
BstYI r'GATC_y
Cuts at: 1 998 1244 1403 1599 2172 2639 2662 2997
Size: 997 246 159 196 573 467 23 335
Cuts at: 2997 3243 4257 4343 4355 4701 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 997 573 467 449 346 335 246
246 196 159 86 23 12
BtgI C'CryG_G
Cuts at: 746 896 1854 746
Size: 150 958 4041
Fragments arranged by size:
4041 958 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 716 3097 3278 3441 716
Size: 2381 181 163 2424
Fragments arranged by size:
2424 2381 181 163
BtsI GCAGTG_nn'
Cuts at: 410 548 714 1069 2390 410
Size: 138 166 355 1321 3169
Fragments arranged by size:
3169 1321 355 166 138
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 580 2853 3713 580
Size: 2273 860 2016
Fragments arranged by size:
2273 2016 860
EaeI y'GGCC_r
Cuts at: 529 917 1427 2732 2906 3297 3324 4671 529
Size: 388 510 1305 174 391 27 1347 1007
Fragments arranged by size:
1347 1305 1007 510 391 388 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5149
EarI CTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4939
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 964 1723 2067 3355 3667 3813 4959 964
Size: 759 344 1288 312 146 1146 1154
Fragments arranged by size:
1288 1154 1146 759 344 312 146
EcoICRI GAG'CTC
Cuts at: 568 693 1290 1574 1616 2159 568
Size: 125 597 284 42 543 3558
Fragments arranged by size:
3558 597 543 284 125 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 437 450 621 680 743 1186 1243 1801
Size: 79 13 171 59 63 443 57 558
Cuts at: 1801 1816 2972 3404 4153 4421 358
Size: 15 1156 432 749 268 1086
Fragments arranged by size:
1156 1086 749 558 443 432 268 171
79 63 59 57 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1929 1929
Size: 5149
EcoO109I rG'GnC_Cy
Cuts at: 237 612 1161 1725 2286 237
Size: 375 549 564 561 3100
Fragments arranged by size:
3100 564 561 549 375
EcoRI G'AATT_C
Cuts at: 2151 2151
Size: 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 1857 3166 228
Size: 1629 1309 2211
Fragments arranged by size:
2211 1629 1309
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5149
HaeII r_GCGC'y
Cuts at: 1191 1862 1914 2581 2829 3853 4832 1191
Size: 671 52 667 248 1024 979 1508
Fragments arranged by size:
1508 1024 979 671 667 248 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 867 899 2989 3019 3021 3051 867
Size: 32 2090 30 2 30 2965
Fragments arranged by size:
2965 2090 32 30 30 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5149
KasI G'GCGC_C
Cuts at: 1858 2577 2825 1858
Size: 719 248 4182
Fragments arranged by size:
4182 719 248
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5149
MluI A'CGCG_T
Cuts at: 4582 4582
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 529 3820 4004 529
Size: 3291 184 1674
Fragments arranged by size:
3291 1674 184
MscI TGG'CCA
Cuts at: 531 2908 531
Size: 2377 2772
MslI CAynn'nnrTG
Cuts at: 223 413 553 790 3136 3263 223
Size: 190 140 237 2346 127 2109
Fragments arranged by size:
2346 2109 237 190 140 127
MspA1I CmG'CkG
Cuts at: 580 678 898 929 1101 1139 1425 1953 2258
Size: 98 220 31 172 38 286 528 305
Cuts at: 2258 2572 2932 3947 4192 580
Size: 314 360 1015 245 1537
Fragments arranged by size:
1537 1015 528 360 314 305 286 245
220 172 98 38 31
NaeI GCC'GGC
Cuts at: 1342 1762 3328 1342
Size: 420 1566 3163
Fragments arranged by size:
3163 1566 420
NarI GG'CG_CC
Cuts at: 1859 2578 2826 1859
Size: 719 248 4182
Fragments arranged by size:
4182 719 248
NcoI C'CATG_G
Cuts at: 746 746
Size: 5149
NgoMIV G'CCGG_C
Cuts at: 1340 1760 3326 1340
Size: 420 1566 3163
Fragments arranged by size:
3163 1566 420
NruI TCG'CGA
Cuts at: 3510 3510
Size: 5149
NspI r_CATG'y
Cuts at: 1086 1495 2200 2700 3609 1086
Size: 409 705 500 909 2626
Fragments arranged by size:
2626 909 705 500 409
PasI CC'CwG_GG
Cuts at: 131 131
Size: 5149
PciI A'CATG_T
Cuts at: 1082 3605 1082
Size: 2523 2626
PflMI CCAn_nnn'nTGG
Cuts at: 131 762 131
Size: 631 4518
PfoI T'CCnGG_A
Cuts at: 1220 1220
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1159 1191 4288 4320 1159
Size: 32 3097 32 1988
Fragments arranged by size:
3097 1988 32 32
PpuMI rG'GwC_Cy
Cuts at: 612 1725 612
Size: 1113 4036
PshAI GACnn'nnGTC
Cuts at: 985 985
Size: 5149
PspOMI G'GGCC_C
Cuts at: 236 236
Size: 5149
PspXI vC'TCGA_Gb
Cuts at: 1646 1646
Size: 5149
PstI C_TGCA'G
Cuts at: 258 1352 1424 1991 2194 2879 258
Size: 1094 72 567 203 685 2528
Fragments arranged by size:
2528 1094 685 567 203 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5149
PvuII CAG'CTG
Cuts at: 580 678 1101 1425 1953 2572 2932 580
Size: 98 423 324 528 619 360 2797
Fragments arranged by size:
2797 619 528 423 360 324 98
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5149
SacI G_AGCT'C
Cuts at: 570 695 1292 1576 1618 2161 570
Size: 125 597 284 42 543 3558
Fragments arranged by size:
3558 597 543 284 125 42
SacII CC_GC'GG
Cuts at: 899 899
Size: 5149
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5149
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4939
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5149
ScaI AGT'ACT
Cuts at: 1258 4666 1258
Size: 3408 1741
SfcI C'TryA_G
Cuts at: 33 254 648 1348 1420 1987 2190 2875 3870
Size: 221 394 700 72 567 203 685 995
Cuts at: 3870 4061 33
Size: 191 1121
Fragments arranged by size:
1121 995 700 685 567 394 221 203
191 72
SfoI GGC'GCC
Cuts at: 1860 2579 2827 1860
Size: 719 248 4182
Fragments arranged by size:
4182 719 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5149
SmlI C'TyrA_G
Cuts at: 1540 1646 1661 1918 2302 3217 3711 3973 1540
Size: 106 15 257 384 915 494 262 2716
Fragments arranged by size:
2716 915 494 384 262 257 106 15
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5149
SphI G_CATG'C
Cuts at: 1495 2200 2700 1495
Size: 705 500 3944
Fragments arranged by size:
3944 705 500
StuI AGG'CCT
Cuts at: 1150 1336 1924 1150
Size: 186 588 4375
Fragments arranged by size:
4375 588 186
StyI C'CwwG_G
Cuts at: 289 746 2281 2635 289
Size: 457 1535 354 2803
Fragments arranged by size:
2803 1535 457 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1198 2730 2863 3322 3533 1198
Size: 1532 133 459 211 2814
Fragments arranged by size:
2814 1532 459 211 133
TatI w'GTAC_w
Cuts at: 1256 1322 1748 4664 1256
Size: 66 426 2916 1741
Fragments arranged by size:
2916 1741 426 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 4666 4698 316
Size: 32 4318 32 767
Fragments arranged by size:
4318 767 32 32
Tth111I GACn'n_nGTC
Cuts at: 739 2944 739
Size: 2205 2944
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1281 1503 2642 1281
Size: 222 1139 3788
Fragments arranged by size:
3788 1139 222
XhoI C'TCGA_G
Cuts at: 1646 1646
Size: 5149
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5149
XmnI GAAnn'nnTTC
Cuts at: 181 1150 1840 181
Size: 969 690 3490
Fragments arranged by size:
3490 969 690
ZraI GAC'GTC
Cuts at: 783 783
Size: 5149
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI BaeI BfrBI BmtI BsiWI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PmlI PsiI PsrI SanDI SexAI
SfiI SgrAI SrfI SspI SwaI