Restriction map for
pF1KSDA1129
(Circular) (Six-base) MAPSORT of: pF1KSDA1129.seq Check: 5100 from: 1 to: 4900
pF1KSDA1129 4900 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 622 1135 1849 622
Size: 513 714 3673
Fragments arranged by size:
3673 714 513
AatII G_ACGT'C
Cuts at: 1252 1252
Size: 4900
AccI GT'mk_AC
Cuts at: 1936 1936
Size: 4900
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4900
AclI AA'CG_TT
Cuts at: 4740 4740
Size: 4900
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 734 2723 3155 3904 4172 494
Size: 240 1989 432 749 268 1222
Fragments arranged by size:
1989 1222 749 432 268 240
AflIII A'CryG_T
Cuts at: 371 837 1169 1697 2250 3356 4333 371
Size: 466 332 528 553 1106 977 938
Fragments arranged by size:
1106 977 938 553 528 466 332
AgeI A'CCGG_T
Cuts at: 1100 3288 1100
Size: 2188 2712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 656 656
Size: 4900
AlwNI CAG_nnn'CTG
Cuts at: 162 426 492 1494 2203 3772 162
Size: 264 66 1002 709 1569 1290
Fragments arranged by size:
1569 1290 1002 709 264 66
ApaLI G'TGCA_C
Cuts at: 3670 3670
Size: 4900
ApoI r'AATT_y
Cuts at: 685 1902 4253 4509 685
Size: 1217 2351 256 1076
Fragments arranged by size:
2351 1217 1076 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4900
AvaI C'yCGr_G
Cuts at: 1918 1918
Size: 4900
AvrII C'CTAG_G
Cuts at: 859 859
Size: 4900
BamHI G'GATC_C
Cuts at: 1765 1923 1765
Size: 158 4742
BanI G'GyrC_C
Cuts at: 1053 1914 2328 2576 2611 1053
Size: 861 414 248 35 3342
Fragments arranged by size:
3342 861 414 248 35
BanII G_rGCy'C
Cuts at: 154 1739 1912 2942 154
Size: 1585 173 1030 2112
Fragments arranged by size:
2112 1585 1030 173
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4652
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3559 4900 2796
Size: 763 1341 2796
Fragments arranged by size:
2796 1341 763
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4900
BglI GCCn_nnn'nGGC
Cuts at: 1568 1568
Size: 4900
BglII A'GATC_T
Cuts at: 2413 4452 2413
Size: 2039 2861
BlpI GC'TnA_GC
Cuts at: 148 1118 1588 2010 148
Size: 970 470 422 3038
Fragments arranged by size:
3038 970 470 422
Bme1580I G_kGCm'C
Cuts at: 2523 2616 3674 2523
Size: 93 1058 3749
Fragments arranged by size:
3749 1058 93
BmgBI CAC'GTC
Cuts at: 279 411 279
Size: 132 4768
BmrI ACTGGGnnnn_n'
Cuts at: 1294 2110 2191 2526 4672 1294
Size: 816 81 335 2146 1522
Fragments arranged by size:
2146 1522 816 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 995
Size: 4900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3447 3745 2074
Size: 879 494 298 3229
Fragments arranged by size:
3229 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4604 4604
Size: 4900
BsaAI yAC'GTr
Cuts at: 1170 1542 1890 2251 1170
Size: 372 348 361 3819
Fragments arranged by size:
3819 372 361 348
BsaBI GATnn'nnATC
Cuts at: 6 400 2412 4451 6
Size: 394 2012 2039 455
Fragments arranged by size:
2039 2012 455 394
BsaHI Gr'CG_yC
Cuts at: 1249 2329 2577 3281 4777 1249
Size: 1080 248 704 1496 1372
Fragments arranged by size:
1496 1372 1080 704 248
BsaWI w'CCGG_w
Cuts at: 1100 2087 2608 3288 3562 3709 4120 1100
Size: 987 521 680 274 147 411 1880
Fragments arranged by size:
1880 987 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1087
Size: 30 4870
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 946 1108 946
Size: 162 4738
BseYI C'CCAG_C
Cuts at: 172 1518 2323 3081 3660 4156 172
Size: 1346 805 758 579 496 916
Fragments arranged by size:
1346 916 805 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 735 1745 600
Size: 135 1010 3755
Fragments arranged by size:
3755 1010 135
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3696 104
Size: 2336 46 1210 1308
Fragments arranged by size:
2336 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 154 818 1282 1301 1501 1558 1843 1912 2690
Size: 664 464 19 200 57 285 69 778
Cuts at: 2690 2880 3674 154
Size: 190 794 1380
Fragments arranged by size:
1380 794 778 664 464 285 200 190
69 57 19
BsmI GAATG_Cn'
Cuts at: 232 232
Size: 4900
Bsp1286I G_dGCh'C
Cuts at: 154 818 1282 1301 1501 1558 1739 1843 1912
Size: 664 464 19 200 57 181 104 69
Cuts at: 1912 2523 2616 2690 2880 2942 3674 154
Size: 611 93 74 190 62 732 1380
Fragments arranged by size:
1380 732 664 611 464 200 190 181
104 93 74 69 62 57 19
BspEI T'CCGG_A
Cuts at: 4120 4120
Size: 4900
BspHI T'CATG_A
Cuts at: 1031 4076 1031
Size: 3045 1855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 1135 1849 1948 2162 2464 2845 622
Size: 513 714 99 214 302 381 2677
Fragments arranged by size:
2677 714 513 381 302 214 99
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4730 4895 50
Size: 3140 54 1486 165 55
Fragments arranged by size:
3140 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1253 2810 1253
Size: 1557 3343
BsrFI r'CCGG_y
Cuts at: 1100 1703 2896 3077 3288 1100
Size: 603 1193 181 211 2712
Fragments arranged by size:
2712 1193 603 211 181
BsrGI T'GTAC_A
Cuts at: 606 606
Size: 4900
BssSI C'ACGA_G
Cuts at: 3169 3529 3169
Size: 360 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 2275 1864
Size: 411 4489
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4900
BstXI CCAn_nnnn'nTGG
Cuts at: 582 582
Size: 4900
BstYI r'GATC_y
Cuts at: 1 1765 1923 2390 2413 2748 2994 4008 4094
Size: 1764 158 467 23 335 246 1014 86
Cuts at: 4094 4106 4452 1
Size: 12 346 449
Fragments arranged by size:
1764 1014 467 449 346 335 246 158
86 23 12
BtgI C'CryG_G
Cuts at: 125 1178 125
Size: 1053 3847
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 370 2848 3029 3192 370
Size: 2478 181 163 2078
Fragments arranged by size:
2478 2078 181 163
BtsI GCAGTG_nn'
Cuts at: 1077 1198 1325 1428 1572 2141 1077
Size: 121 127 103 144 569 3836
Fragments arranged by size:
3836 569 144 127 121 103
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4900
DraIII CAC_nnn'GTG
Cuts at: 321 321
Size: 4900
DrdI GACnn_nn'nnGTC
Cuts at: 871 2604 3464 871
Size: 1733 860 2307
Fragments arranged by size:
2307 1733 860
EaeI y'GGCC_r
Cuts at: 1047 1164 2483 2657 3048 3075 4422 1047
Size: 117 1319 174 391 27 1347 1525
Fragments arranged by size:
1525 1347 1319 391 174 117 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4900
EarI CTCTTCn'nnn_
Cuts at: 470 749 1633 2921 3131 470
Size: 279 884 1288 210 2239
Fragments arranged by size:
2239 1288 884 279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1431 1561 1863 3106 3418 3564 4710 1431
Size: 130 302 1243 312 146 1146 1621
Fragments arranged by size:
1621 1243 1146 312 302 146 130
EcoICRI GAG'CTC
Cuts at: 152 1910 152
Size: 1758 3142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 734 995 2723 3155 3904 4172 494
Size: 240 261 1728 432 749 268 1222
Fragments arranged by size:
1728 1222 749 432 268 261 240
EcoNI CCTnn'n_nnAGG
Cuts at: 934 1024 934
Size: 90 4810
EcoO109I rG'GnC_Cy
Cuts at: 2037 2037
Size: 4900
EcoRI G'AATT_C
Cuts at: 1902 1902
Size: 4900
EcoRV GAT'ATC
Cuts at: 725 725
Size: 4900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 706 2917 706
Size: 2211 2689
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4900
HaeII r_GCGC'y
Cuts at: 1275 2332 2580 3604 4583 1275
Size: 1057 248 1024 979 1592
Fragments arranged by size:
1592 1057 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 836 868 1750 1782 2740 2770 2772 2802 836
Size: 32 882 32 958 30 2 30 2934
Fragments arranged by size:
2934 958 882 32 32 30 30 2
HincII GTy'rAC
Cuts at: 210 1937 210
Size: 1727 3173
HindIII A'AGCT_T
Cuts at: 893 893
Size: 4900
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4652
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4900
MfeI C'AATT_G
Cuts at: 576 576
Size: 4900
MluI A'CGCG_T
Cuts at: 4333 4333
Size: 4900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 867 1560 1731 1852 3571 3755 867
Size: 693 171 121 1719 184 2012
Fragments arranged by size:
2012 1719 693 184 171 121
MscI TGG'CCA
Cuts at: 1166 2659 1166
Size: 1493 3407
MslI CAynn'nnrTG
Cuts at: 1036 1172 1696 2887 3014 1036
Size: 136 524 1191 127 2922
Fragments arranged by size:
2922 1191 524 136 127
MspA1I CmG'CkG
Cuts at: 267 1294 1599 2009 2323 2683 3698 3943 267
Size: 1027 305 410 314 360 1015 245 1224
Fragments arranged by size:
1224 1027 1015 410 360 314 305 245
NaeI GCC'GGC
Cuts at: 1705 3079 1705
Size: 1374 3526
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4652
NcoI C'CATG_G
Cuts at: 125 125
Size: 4900
NgoMIV G'CCGG_C
Cuts at: 1703 3077 1703
Size: 1374 3526
NruI TCG'CGA
Cuts at: 3261 3261
Size: 4900
NspI r_CATG'y
Cuts at: 375 841 1266 1701 1951 2451 3360 375
Size: 466 425 435 250 500 909 1915
Fragments arranged by size:
1915 909 500 466 435 425 250
PciI A'CATG_T
Cuts at: 371 837 1697 3356 371
Size: 466 860 1659 1915
Fragments arranged by size:
1915 1659 860 466
PflMI CCAn_nnn'nTGG
Cuts at: 321 1385 1532 321
Size: 1064 147 3689
Fragments arranged by size:
3689 1064 147
PfoI T'CCnGG_A
Cuts at: 1659 1659
Size: 4900
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4900
PmlI CAC'GTG
Cuts at: 1170 1170
Size: 4900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4039 4071 4039
Size: 32 4868
PshAI GACnn'nnGTC
Cuts at: 1012 1012
Size: 4900
PstI C_TGCA'G
Cuts at: 340 518 557 632 1945 2630 340
Size: 178 39 75 1313 685 2610
Fragments arranged by size:
2610 1313 685 178 75 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4900
PvuII CAG'CTG
Cuts at: 267 1599 2323 2683 267
Size: 1332 724 360 2484
Fragments arranged by size:
2484 1332 724 360
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4900
SacI G_AGCT'C
Cuts at: 154 1912 154
Size: 1758 3142
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4900
SapI GCTCTTCn'nnn_
Cuts at: 1633 2921 3131 1633
Size: 1288 210 3402
Fragments arranged by size:
3402 1288 210
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4900
ScaI AGT'ACT
Cuts at: 1341 1792 4417 1341
Size: 451 2625 1824
Fragments arranged by size:
2625 1824 451
SexAI A'CCwGG_T
Cuts at: 707 707
Size: 4900
SfcI C'TryA_G
Cuts at: 33 336 514 553 628 1365 1941 2626 3621
Size: 303 178 39 75 737 576 685 995
Cuts at: 3621 3812 33
Size: 191 1121
Fragments arranged by size:
1121 995 737 685 576 303 191 178
75 39
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4652
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4900
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4900
SmlI C'TyrA_G
Cuts at: 2053 2968 3462 3724 2053
Size: 915 494 262 3229
Fragments arranged by size:
3229 915 494 262
SnaBI TAC'GTA
Cuts at: 1890 1890
Size: 4900
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4900
SphI G_CATG'C
Cuts at: 1266 1951 2451 1266
Size: 685 500 3715
Fragments arranged by size:
3715 685 500
StyI C'CwwG_G
Cuts at: 125 859 991 1025 1058 2032 2386 125
Size: 734 132 34 33 974 354 2639
Fragments arranged by size:
2639 974 734 354 132 34 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1071 1417 2481 2614 3073 3284 1071
Size: 346 1064 133 459 211 2687
Fragments arranged by size:
2687 1064 459 346 211 133
TatI w'GTAC_w
Cuts at: 606 1339 1511 1790 4415 606
Size: 733 172 279 2625 1091
Fragments arranged by size:
2625 1091 733 279 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 697 729 1087 1119 4417 4449 697
Size: 32 358 32 3298 32 1148
Fragments arranged by size:
3298 1148 358 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4900
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 2393 579
Size: 1814 3086
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4900
XmnI GAAnn'nnTTC
Cuts at: 232 686 232
Size: 454 4446
ZraI GAC'GTC
Cuts at: 1250 1250
Size: 4900
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI FspAI
HpaI NdeI NheI NotI NsiI PacI PasI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI StuI SwaI XhoI