Restriction map for
pF1KSDA1133
(Circular) (Six-base) MAPSORT of: pF1KSDA1133.seq Check: 5232 from: 1 to: 5647
pF1KSDA1133 5647 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 820 1813 2172 820
Size: 993 359 4295
Fragments arranged by size:
4295 993 359
AccI GT'mk_AC
Cuts at: 1532 2683 1532
Size: 1151 4496
Acc65I G'GTAC_C
Cuts at: 2661 2661
Size: 5647
AclI AA'CG_TT
Cuts at: 5487 5487
Size: 5647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 571 1395 3470 3902 4651 4919 362
Size: 209 824 2075 432 749 268 1090
Fragments arranged by size:
2075 1090 824 749 432 268 209
AflIII A'CryG_T
Cuts at: 2997 4103 5080 2997
Size: 1106 977 3564
Fragments arranged by size:
3564 1106 977
AgeI A'CCGG_T
Cuts at: 764 4035 764
Size: 3271 2376
AleI CACnn'nnGTG
Cuts at: 1360 1360
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2213 2950 4519 2213
Size: 737 1569 3341
Fragments arranged by size:
3341 1569 737
ApaI G_GGCC'C
Cuts at: 669 1309 1774 1859 2036 2292 2484 669
Size: 640 465 85 177 256 192 3832
Fragments arranged by size:
3832 640 465 256 192 177 85
ApaLI G'TGCA_C
Cuts at: 940 1363 1732 4417 940
Size: 423 369 2685 2170
Fragments arranged by size:
2685 2170 423 369
ApoI r'AATT_y
Cuts at: 570 2649 5000 5256 570
Size: 2079 2351 256 961
Fragments arranged by size:
2351 2079 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5647
AvaI C'yCGr_G
Cuts at: 428 1301 1662 1671 1714 1785 1885 2284 2291
Size: 873 361 9 43 71 100 399 7
Cuts at: 2291 2444 2625 2665 428
Size: 153 181 40 3410
Fragments arranged by size:
3410 873 399 361 181 153 100 71
43 40 9 7
AvrII C'CTAG_G
Cuts at: 243 243
Size: 5647
BamHI G'GATC_C
Cuts at: 437 2670 437
Size: 2233 3414
BanI G'GyrC_C
Cuts at: 644 2661 3075 3323 3358 644
Size: 2017 414 248 35 2933
Fragments arranged by size:
2933 2017 414 248 35
BanII G_rGCy'C
Cuts at: 340 669 1309 1636 1663 1705 1724 1774 1859
Size: 329 640 327 27 42 19 50 85
Cuts at: 1859 1886 1973 2036 2053 2075 2292 2463 2484
Size: 27 87 63 17 22 217 171 21
Cuts at: 2484 2659 3689 340
Size: 175 1030 2298
Fragments arranged by size:
2298 1030 640 329 327 217 175 171
87 85 63 50 42 27 27 22
21 19 17
BbeI G_GCGC'C
Cuts at: 648 3079 3327 648
Size: 2431 248 2968
Fragments arranged by size:
2968 2431 248
BbsI GAAGACnn'nnnn_
Cuts at: 350 350
Size: 5647
BbvCI CC'TCA_GC
Cuts at: 1255 1594 1255
Size: 339 5308
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3543 4306 5647 2403
Size: 1140 763 1341 2403
Fragments arranged by size:
2403 1341 1140 763
BclI T'GATC_A
Cuts at: 321 3165 321
Size: 2844 2803
BglI GCCn_nnn'nGGC
Cuts at: 1651 2584 1651
Size: 933 4714
BglII A'GATC_T
Cuts at: 2599 3160 5199 2599
Size: 561 2039 3047
Fragments arranged by size:
3047 2039 561
BlpI GC'TnA_GC
Cuts at: 2757 2757
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 669 944 1309 1367 1736 1774 1859 2036 2292
Size: 275 365 58 369 38 85 177 256
Cuts at: 2292 2484 2547 3270 3363 4421 669
Size: 192 63 723 93 1058 1895
Fragments arranged by size:
1895 1058 723 369 365 275 256 192
177 93 85 63 58 38
BmgBI CAC'GTC
Cuts at: 695 1403 695
Size: 708 4939
BmrI ACTGGGnnnn_n'
Cuts at: 145 1209 2070 2857 2938 3273 5419 145
Size: 1064 861 787 81 335 2146 373
Fragments arranged by size:
2146 1064 861 787 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 4012 2247
Size: 1765 3882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1150 1408 1500 1834 1864 2533 736
Size: 414 258 92 334 30 669 3850
Fragments arranged by size:
3850 669 414 334 258 92 30
Bpu10I CC'TnA_GC
Cuts at: 1255 1594 2299 1255
Size: 339 705 4603
Fragments arranged by size:
4603 705 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2821 3700 4194 4492 337
Size: 2484 879 494 298 1492
Fragments arranged by size:
2484 1492 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 516 868 1216 5351 516
Size: 352 348 4135 812
Fragments arranged by size:
4135 812 352 348
BsaAI yAC'GTr
Cuts at: 892 1368 2637 2998 892
Size: 476 1269 361 3541
Fragments arranged by size:
3541 1269 476 361
BsaBI GATnn'nnATC
Cuts at: 6 403 3159 5198 6
Size: 397 2756 2039 455
Fragments arranged by size:
2756 2039 455 397
BsaHI Gr'CG_yC
Cuts at: 645 3076 3324 4028 5524 645
Size: 2431 248 704 1496 768
Fragments arranged by size:
2431 1496 768 704 248
BsaWI w'CCGG_w
Cuts at: 764 2505 2834 3355 4035 4309 4456 4867 764
Size: 1741 329 521 680 274 147 411 1544
Fragments arranged by size:
1741 1544 680 521 411 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1264 1294 1498 1528 1623 1653 2062 2092 2621
Size: 30 204 30 95 30 409 30 529
Cuts at: 2621 2651 1264
Size: 30 4260
Fragments arranged by size:
4260 529 409 204 95 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 443 751 1732 1827 2467 2524 443
Size: 308 981 95 640 57 3566
Fragments arranged by size:
3566 981 640 308 95 57
BseYI C'CCAG_C
Cuts at: 182 960 1149 1747 1973 1983 3070 3828 4407
Size: 778 189 598 226 10 1087 758 579
Cuts at: 4407 4903 182
Size: 496 926
Fragments arranged by size:
1087 926 778 758 598 579 496 226
189 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 613 1461 1521 2263 401
Size: 212 848 60 742 3785
Fragments arranged by size:
3785 848 742 212 60
BsiEI CG_ry'CG
Cuts at: 104 936 3187 3233 4443 104
Size: 832 2251 46 1210 1308
Fragments arranged by size:
2251 1308 1210 832 46
BsiHKAI G_wGCw'C
Cuts at: 944 1136 1367 1724 1736 1850 2659 3437 3627
Size: 192 231 357 12 114 809 778 190
Cuts at: 3627 4421 944
Size: 794 2170
Fragments arranged by size:
2170 809 794 778 357 231 192 190
114 12
Bsp1286I G_dGCh'C
Cuts at: 340 669 944 1136 1309 1367 1636 1663 1705
Size: 329 275 192 173 58 269 27 42
Cuts at: 1705 1724 1736 1774 1850 1859 1886 1973 2036
Size: 19 12 38 76 9 27 87 63
Cuts at: 2036 2053 2075 2292 2463 2484 2547 2659 3270
Size: 17 22 217 171 21 63 112 611
Cuts at: 3270 3363 3437 3627 3689 4421 340
Size: 93 74 190 62 732 1566
Fragments arranged by size:
1566 732 611 329 275 269 217 192
190 173 171 112 93 87 76 74
63 63 62 58 42 38 27 27
22 21 19 17 12 9
BspEI T'CCGG_A
Cuts at: 2505 4867 2505
Size: 2362 3285
BspHI T'CATG_A
Cuts at: 4823 4823
Size: 5647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1692 1813 2172 2695 2909 3211 3592 820
Size: 872 121 359 523 214 302 381 2875
Fragments arranged by size:
2875 872 523 381 359 302 214 121
BsrBI CCG'CTC
Cuts at: 50 351 1114 1331 1394 1433 2457 3937 3991
Size: 301 763 217 63 39 1024 1480 54
Cuts at: 3991 5477 5642 50
Size: 1486 165 55
Fragments arranged by size:
1486 1480 1024 763 301 217 165 63
55 54 39
BsrDI GCAATG_nn'
Cuts at: 3557 3557
Size: 5647
BsrFI r'CCGG_y
Cuts at: 360 764 918 1025 3643 3824 4035 360
Size: 404 154 107 2618 181 211 1972
Fragments arranged by size:
2618 1972 404 211 181 154 107
BssSI C'ACGA_G
Cuts at: 1939 3916 4276 1939
Size: 1977 360 3310
Fragments arranged by size:
3310 1977 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2213 3022 2213
Size: 809 4838
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5647
BstEII G'GTnAC_C
Cuts at: 909 909
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 5647
BstYI r'GATC_y
Cuts at: 1 437 2599 2670 3137 3160 3495 3741 4755
Size: 436 2162 71 467 23 335 246 1014
Cuts at: 4755 4841 4853 5199 1
Size: 86 12 346 449
Fragments arranged by size:
2162 1014 467 449 436 346 335 246
86 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 1915 1915
Size: 5647
BtgI C'CryG_G
Cuts at: 993 1019 1358 2190 2416 993
Size: 26 339 832 226 4224
Fragments arranged by size:
4224 832 339 226 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3595 3776 3939 3595
Size: 181 163 5303
Fragments arranged by size:
5303 181 163
BtsI GCAGTG_nn'
Cuts at: 1341 1449 2573 2888 1341
Size: 108 1124 315 4100
Fragments arranged by size:
4100 1124 315 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1161 1196 1161
Size: 35 5612
DraI TTT'AAA
Cuts at: 2644 2644
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 3351 4211 3351
Size: 860 4787
EaeI y'GGCC_r
Cuts at: 933 1109 1182 1425 1454 1497 1765 3230 3404
Size: 176 73 243 29 43 268 1465 174
Cuts at: 3404 3795 3822 5169 933
Size: 391 27 1347 1411
Fragments arranged by size:
1465 1411 1347 391 268 243 176 174
73 43 29 27
EagI C'GGCC_G
Cuts at: 933 3230 933
Size: 2297 3350
EarI CTCTTCn'nnn_
Cuts at: 155 1354 2168 2444 3668 3878 155
Size: 1199 814 276 1224 210 1924
Fragments arranged by size:
1924 1224 1199 814 276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2213 3853 4165 4311 5457 2213
Size: 1640 312 146 1146 2403
Fragments arranged by size:
2403 1640 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1722 2657 1722
Size: 935 4712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 571 736 1150 1395 1408 1500 1834 1864
Size: 209 165 414 245 13 92 334 30
Cuts at: 1864 2533 3470 3902 4651 4919 362
Size: 669 937 432 749 268 1090
Fragments arranged by size:
1090 937 749 669 432 414 334 268
245 209 165 92 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 247 1164 2044 247
Size: 917 880 3850
Fragments arranged by size:
3850 917 880
EcoO109I rG'GnC_Cy
Cuts at: 665 666 1251 1305 1681 1771 1855 1922 2032
Size: 1 585 54 376 90 84 67 110
Cuts at: 2032 2033 2288 2295 2369 2430 2480 2481 2784
Size: 1 255 7 74 61 50 1 303
Cuts at: 2784 665
Size: 3528
Fragments arranged by size:
3528 585 376 303 255 110 90 84
74 67 61 54 50 7 1 1
1
EcoRI G'AATT_C
Cuts at: 570 2649 570
Size: 2079 3568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 5647
FspI TGC'GCA
Cuts at: 3426 3426
Size: 5647
HaeII r_GCGC'y
Cuts at: 648 2000 3079 3327 4351 5330 648
Size: 1352 1079 248 1024 979 965
Fragments arranged by size:
1352 1079 1024 979 965 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 422 454 1264 1296 1623 1655 1783 1815 1933
Size: 32 810 32 327 32 128 32 118
Cuts at: 1933 1965 2062 2094 3487 3517 3519 3549 422
Size: 32 97 32 1393 30 2 30 2520
Fragments arranged by size:
2520 1393 810 327 128 118 97 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 411 2684 411
Size: 2273 3374
KasI G'GCGC_C
Cuts at: 644 3075 3323 644
Size: 2431 248 2968
Fragments arranged by size:
2968 2431 248
KpnI G_GTAC'C
Cuts at: 2665 2665
Size: 5647
MluI A'CGCG_T
Cuts at: 5080 5080
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 4318 4502 722
Size: 3596 184 1867
Fragments arranged by size:
3596 1867 184
MscI TGG'CCA
Cuts at: 3406 3406
Size: 5647
MslI CAynn'nnrTG
Cuts at: 1360 1739 3634 3761 1360
Size: 379 1895 127 3246
Fragments arranged by size:
3246 1895 379 127
MspA1I CmG'CkG
Cuts at: 270 327 1121 1415 2008 2192 2210 2756 3070
Size: 57 794 294 593 184 18 546 314
Cuts at: 3070 3430 4445 4690 270
Size: 360 1015 245 1227
Fragments arranged by size:
1227 1015 794 593 546 360 314 294
245 184 57 18
NaeI GCC'GGC
Cuts at: 3826 3826
Size: 5647
NarI GG'CG_CC
Cuts at: 645 3076 3324 645
Size: 2431 248 2968
Fragments arranged by size:
2968 2431 248
NcoI C'CATG_G
Cuts at: 1019 1019
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 3824 3824
Size: 5647
NruI TCG'CGA
Cuts at: 4008 4008
Size: 5647
NspI r_CATG'y
Cuts at: 2698 3198 4107 2698
Size: 500 909 4238
Fragments arranged by size:
4238 909 500
PasI CC'CwG_GG
Cuts at: 2091 2091
Size: 5647
PciI A'CATG_T
Cuts at: 4103 4103
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1107 1107
Size: 5647
PfoI T'CCnGG_A
Cuts at: 2549 2549
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2644 2644
Size: 5647
PmlI CAC'GTG
Cuts at: 892 1368 892
Size: 476 5171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 354 4786 4818 322
Size: 32 4432 32 1151
Fragments arranged by size:
4432 1151 32 32
PpuMI rG'GwC_Cy
Cuts at: 1681 1922 2295 2369 1681
Size: 241 373 74 4959
Fragments arranged by size:
4959 373 241 74
PspOMI G'GGCC_C
Cuts at: 665 1305 1770 1855 2032 2288 2480 665
Size: 640 465 85 177 256 192 3832
Fragments arranged by size:
3832 640 465 256 192 177 85
PspXI vC'TCGA_Gb
Cuts at: 428 1885 428
Size: 1457 4190
PstI C_TGCA'G
Cuts at: 285 803 1810 2592 2692 3377 285
Size: 518 1007 782 100 685 2555
Fragments arranged by size:
2555 1007 782 685 518 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5647
PvuII CAG'CTG
Cuts at: 327 1415 2008 3070 3430 327
Size: 1088 593 1062 360 2544
Fragments arranged by size:
2544 1088 1062 593 360
RsrII CG'GwC_CG
Cuts at: 3840 3840
Size: 5647
SacI G_AGCT'C
Cuts at: 1724 2659 1724
Size: 935 4712
SacII CC_GC'GG
Cuts at: 2193 2193
Size: 5647
SalI G'TCGA_C
Cuts at: 2682 2682
Size: 5647
SanDI GG'GwC_CC
Cuts at: 2369 2369
Size: 5647
SapI GCTCTTCn'nnn_
Cuts at: 1354 3668 3878 1354
Size: 2314 210 3123
Fragments arranged by size:
3123 2314 210
SbfI CC_TGCA'GG
Cuts at: 2692 2692
Size: 5647
ScaI AGT'ACT
Cuts at: 5164 5164
Size: 5647
SfcI C'TryA_G
Cuts at: 33 281 799 1806 1824 2588 2688 3373 4368
Size: 248 518 1007 18 764 100 685 995
Cuts at: 4368 4559 33
Size: 191 1121
Fragments arranged by size:
1121 1007 995 764 685 518 248 191
100 18
SfoI GGC'GCC
Cuts at: 646 3077 3325 646
Size: 2431 248 2968
Fragments arranged by size:
2968 2431 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5647
SmaI CCC'GGG
Cuts at: 1303 2286 2293 2446 2627 2667 1303
Size: 983 7 153 181 40 4283
Fragments arranged by size:
4283 983 181 153 40 7
SmlI C'TyrA_G
Cuts at: 352 428 1885 2800 3715 4209 4471 352
Size: 76 1457 915 915 494 262 1528
Fragments arranged by size:
1528 1457 915 915 494 262 76
SnaBI TAC'GTA
Cuts at: 2637 2637
Size: 5647
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5647
SphI G_CATG'C
Cuts at: 2698 3198 2698
Size: 500 5147
StyI C'CwwG_G
Cuts at: 243 515 1019 1196 1762 2364 2779 3133 243
Size: 272 504 177 566 602 415 354 2757
Fragments arranged by size:
2757 602 566 504 415 354 272 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 146 2610 3228 3361 3820 4031 146
Size: 2464 618 133 459 211 1762
Fragments arranged by size:
2464 1762 618 459 211 133
TatI w'GTAC_w
Cuts at: 264 722 757 2246 5162 264
Size: 458 35 1489 2916 749
Fragments arranged by size:
2916 1489 749 458 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1498 1530 5164 5196 1498
Size: 32 3634 32 1949
Fragments arranged by size:
3634 1949 32 32
Tth111I GACn'n_nGTC
Cuts at: 563 3442 563
Size: 2879 2768
XbaI T'CTAG_A
Cuts at: 600 2676 600
Size: 2076 3571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 582 1524 1809 3140 132
Size: 450 942 285 1331 2639
Fragments arranged by size:
2639 1331 942 450 285
XhoI C'TCGA_G
Cuts at: 428 1885 428
Size: 1457 4190
XmaI C'CCGG_G
Cuts at: 1301 2284 2291 2444 2625 2665 1301
Size: 983 7 153 181 40 4283
Fragments arranged by size:
4283 983 181 153 40 7
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PshAI PsiI
PsrI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XmnI ZraI