Restriction map for
pF1KSDA1135
(Circular) (Six-base) MAPSORT of: pF1KSDA1135.seq Check: 8971 from: 1 to: 5407
pF1KSDA1135 5407 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1449 1449
Size: 5407
AccI GT'mk_AC
Cuts at: 1549 2443 1549
Size: 894 4513
Acc65I G'GTAC_C
Cuts at: 2421 2421
Size: 5407
AclI AA'CG_TT
Cuts at: 5247 5247
Size: 5407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1604 2354 3230 3662 4411 4679 1604
Size: 750 876 432 749 268 2332
Fragments arranged by size:
2332 876 750 749 432 268
AflII C'TTAA_G
Cuts at: 859 982 859
Size: 123 5284
AflIII A'CryG_T
Cuts at: 2336 2757 3863 4840 2336
Size: 421 1106 977 2903
Fragments arranged by size:
2903 1106 977 421
AgeI A'CCGG_T
Cuts at: 3795 3795
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1481 2017 1481
Size: 536 4871
AlwNI CAG_nnn'CTG
Cuts at: 1731 1833 2710 4279 1731
Size: 102 877 1569 2859
Fragments arranged by size:
2859 1569 877 102
ApaI G_GGCC'C
Cuts at: 2307 2307
Size: 5407
ApaLI G'TGCA_C
Cuts at: 791 4177 791
Size: 3386 2021
ApoI r'AATT_y
Cuts at: 1630 1768 2409 4760 5016 1630
Size: 138 641 2351 256 2021
Fragments arranged by size:
2351 2021 641 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 5407
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5407
AvaI C'yCGr_G
Cuts at: 202 233 2425 202
Size: 31 2192 3184
Fragments arranged by size:
3184 2192 31
BamHI G'GATC_C
Cuts at: 2430 2430
Size: 5407
BanI G'GyrC_C
Cuts at: 406 420 1461 2421 2835 3083 3118 406
Size: 14 1041 960 414 248 35 2695
Fragments arranged by size:
2695 1041 960 414 248 35 14
BanII G_rGCy'C
Cuts at: 203 232 818 1494 2307 2419 3449 203
Size: 29 586 676 813 112 1030 2161
Fragments arranged by size:
2161 1030 813 676 586 112 29
BbeI G_GCGC'C
Cuts at: 410 424 2839 3087 410
Size: 14 2415 248 2730
Fragments arranged by size:
2730 2415 248 14
BciVI GTATCCnnnnn_n'
Cuts at: 3303 4066 5407 3303
Size: 763 1341 3303
Fragments arranged by size:
3303 1341 763
BclI T'GATC_A
Cuts at: 2925 2925
Size: 5407
BfrBI ATG'CAT
Cuts at: 1818 1818
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 149 1743 149
Size: 1594 3813
BglII A'GATC_T
Cuts at: 2390 2920 4959 2390
Size: 530 2039 2838
Fragments arranged by size:
2838 2039 530
BlpI GC'TnA_GC
Cuts at: 244 2517 244
Size: 2273 3134
Bme1580I G_kGCm'C
Cuts at: 795 936 2307 3030 3123 4181 795
Size: 141 1371 723 93 1058 2021
Fragments arranged by size:
2021 1371 1058 723 141 93
BmrI ACTGGGnnnn_n'
Cuts at: 448 939 1774 2617 2698 3033 5179 448
Size: 491 835 843 81 335 2146 676
Fragments arranged by size:
2146 843 835 676 491 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3772 3772
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 417 1618 2167 2232 271
Size: 146 1201 549 65 3446
Fragments arranged by size:
3446 1201 549 146 65
Bpu10I CC'TnA_GC
Cuts at: 131 131
Size: 5407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1783 2581 3460 3954 4252 1783
Size: 798 879 494 298 2938
Fragments arranged by size:
2938 879 798 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5111 5111
Size: 5407
BsaAI yAC'GTr
Cuts at: 2397 2758 2397
Size: 361 5046
BsaBI GATnn'nnATC
Cuts at: 6 2919 4958 6
Size: 2913 2039 455
Fragments arranged by size:
2913 2039 455
BsaHI Gr'CG_yC
Cuts at: 407 421 2836 3084 3788 5284 407
Size: 14 2415 248 704 1496 530
Fragments arranged by size:
2415 1496 704 530 248 14
BsaWI w'CCGG_w
Cuts at: 2594 3115 3795 4069 4216 4627 2594
Size: 521 680 274 147 411 3374
Fragments arranged by size:
3374 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 1214 1244 1591 1621 283
Size: 30 901 30 347 30 4069
Fragments arranged by size:
4069 901 347 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 304 1306 1474 2133 268
Size: 36 1002 168 659 3542
Fragments arranged by size:
3542 1002 659 168 36
BseYI C'CCAG_C
Cuts at: 1655 2830 3588 4167 4663 1655
Size: 1175 758 579 496 2399
Fragments arranged by size:
2399 1175 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 1525 1451
Size: 74 5333
BsiEI CG_ry'CG
Cuts at: 104 224 380 431 2947 2993 4203 104
Size: 120 156 51 2516 46 1210 1308
Fragments arranged by size:
2516 1308 1210 156 120 51 46
BsiHKAI G_wGCw'C
Cuts at: 795 818 1137 1984 2218 2419 3197 3387 4181
Size: 23 319 847 234 201 778 190 794
Cuts at: 4181 795
Size: 2021
Fragments arranged by size:
2021 847 794 778 319 234 201 190
23
BsmI GAATG_Cn'
Cuts at: 1792 1792
Size: 5407
BsmBI CGTCTCn'nnnn_
Cuts at: 1659 1677 1659
Size: 18 5389
Bsp1286I G_dGCh'C
Cuts at: 203 232 795 818 936 1137 1494 1984 2218
Size: 29 563 23 118 201 357 490 234
Cuts at: 2218 2307 2419 3030 3123 3197 3387 3449 4181
Size: 89 112 611 93 74 190 62 732
Cuts at: 4181 203
Size: 1429
Fragments arranged by size:
1429 732 611 563 490 357 234 201
190 118 112 93 89 74 62 29
23
BspEI T'CCGG_A
Cuts at: 4627 4627
Size: 5407
BspHI T'CATG_A
Cuts at: 722 1137 4583 722
Size: 415 3446 1546
Fragments arranged by size:
3446 1546 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1449 2455 2669 2971 3352 1449
Size: 1006 214 302 381 3504
Fragments arranged by size:
3504 1006 381 302 214
BsrBI CCG'CTC
Cuts at: 50 999 1757 3697 3751 5237 5402 50
Size: 949 758 1940 54 1486 165 55
Fragments arranged by size:
1940 1486 949 758 165 55 54
BsrDI GCAATG_nn'
Cuts at: 639 1322 2129 3317 639
Size: 683 807 1188 2729
Fragments arranged by size:
2729 1188 807 683
BsrFI r'CCGG_y
Cuts at: 211 1974 2273 3403 3584 3795 211
Size: 1763 299 1130 181 211 1823
Fragments arranged by size:
1823 1763 1130 299 211 181
BsrGI T'GTAC_A
Cuts at: 269 269
Size: 5407
BssHII G'CGCG_C
Cuts at: 333 333
Size: 5407
BssSI C'ACGA_G
Cuts at: 3676 4036 3676
Size: 360 5047
BstAPI GCAn_nnn'nTGC
Cuts at: 799 1731 2782 799
Size: 932 1051 3424
Fragments arranged by size:
3424 1051 932
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 615 615
Size: 5407
BstYI r'GATC_y
Cuts at: 1 257 386 1162 2390 2430 2897 2920 3255
Size: 256 129 776 1228 40 467 23 335
Cuts at: 3255 3501 4515 4601 4613 4959 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1228 1014 776 467 449 346 335 256
246 129 86 40 23 12
BtgI C'CryG_G
Cuts at: 125 163 193 425 1216 1222 2219 125
Size: 38 30 232 791 6 997 3313
Fragments arranged by size:
3313 997 791 232 38 30 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 902 3355 3536 3699 902
Size: 2453 181 163 2610
Fragments arranged by size:
2610 2453 181 163
BtsI GCAGTG_nn'
Cuts at: 182 2102 2648 182
Size: 1920 546 2941
Fragments arranged by size:
2941 1920 546
ClaI AT'CG_AT
Cuts at: 596 596
Size: 5407
DraI TTT'AAA
Cuts at: 1178 2404 1178
Size: 1226 4181
DraIII CAC_nnn'GTG
Cuts at: 369 369
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 409 3111 3971 409
Size: 2702 860 1845
Fragments arranged by size:
2702 1845 860
EaeI y'GGCC_r
Cuts at: 221 377 428 605 1219 1321 1502 2116 2291
Size: 156 51 177 614 102 181 614 175
Cuts at: 2291 2990 3164 3555 3582 4929 221
Size: 699 174 391 27 1347 699
Fragments arranged by size:
1347 699 699 614 614 391 181 177
175 174 156 102 51 27
EagI C'GGCC_G
Cuts at: 221 377 428 2990 221
Size: 156 51 2562 2638
Fragments arranged by size:
2638 2562 156 51
EarI CTCTTCn'nnn_
Cuts at: 905 1580 2036 3428 3638 905
Size: 675 456 1392 210 2674
Fragments arranged by size:
2674 1392 675 456 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3613 3925 4071 5217 3613
Size: 312 146 1146 3803
Fragments arranged by size:
3803 1146 312 146
EcoICRI GAG'CTC
Cuts at: 816 2417 816
Size: 1601 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 417 1604 1618 2167 2232 2354 3230 3662
Size: 146 1187 14 549 65 122 876 432
Cuts at: 3662 4411 4679 271
Size: 749 268 999
Fragments arranged by size:
1187 999 876 749 549 432 268 146
122 65 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1837 1837
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 1830 2050 2303 2350 2544 1830
Size: 220 253 47 194 4693
Fragments arranged by size:
4693 253 220 194 47
EcoRI G'AATT_C
Cuts at: 1768 2409 1768
Size: 641 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 874 1923 3424 874
Size: 1049 1501 2857
Fragments arranged by size:
2857 1501 1049
FspI TGC'GCA
Cuts at: 3186 3186
Size: 5407
HaeII r_GCGC'y
Cuts at: 410 424 2839 3087 4111 5090 410
Size: 14 2415 248 1024 979 727
Fragments arranged by size:
2415 1024 979 727 248 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1623 3247 3277 3279 3309 1591
Size: 32 1624 30 2 30 3689
Fragments arranged by size:
3689 1624 32 30 30 2
HincII GTy'rAC
Cuts at: 1550 2444 1550
Size: 894 4513
KasI G'GCGC_C
Cuts at: 406 420 2835 3083 406
Size: 14 2415 248 2730
Fragments arranged by size:
2730 2415 248 14
KpnI G_GTAC'C
Cuts at: 2425 2425
Size: 5407
MluI A'CGCG_T
Cuts at: 4840 4840
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4078 4262 4078
Size: 184 5223
MscI TGG'CCA
Cuts at: 607 2293 3166 607
Size: 1686 873 2848
Fragments arranged by size:
2848 1686 873
MslI CAynn'nnrTG
Cuts at: 2224 3394 3521 2224
Size: 1170 127 4110
Fragments arranged by size:
4110 1170 127
MspA1I CmG'CkG
Cuts at: 165 195 427 1473 2516 2830 3190 4205 4450
Size: 30 232 1046 1043 314 360 1015 245
Cuts at: 4450 165
Size: 1122
Fragments arranged by size:
1122 1046 1043 1015 360 314 245 232
30
NaeI GCC'GGC
Cuts at: 213 2275 3586 213
Size: 2062 1311 2034
Fragments arranged by size:
2062 2034 1311
NarI GG'CG_CC
Cuts at: 407 421 2836 3084 407
Size: 14 2415 248 2730
Fragments arranged by size:
2730 2415 248 14
NcoI C'CATG_G
Cuts at: 125 2219 125
Size: 2094 3313
NgoMIV G'CCGG_C
Cuts at: 211 2273 3584 211
Size: 2062 1311 2034
Fragments arranged by size:
2062 2034 1311
NruI TCG'CGA
Cuts at: 3768 3768
Size: 5407
NsiI A_TGCA'T
Cuts at: 1820 1820
Size: 5407
NspI r_CATG'y
Cuts at: 1335 2340 2458 2958 3867 1335
Size: 1005 118 500 909 2875
Fragments arranged by size:
2875 1005 909 500 118
PciI A'CATG_T
Cuts at: 2336 3863 2336
Size: 1527 3880
PflMI CCAn_nnn'nTGG
Cuts at: 1833 1833
Size: 5407
PfoI T'CCnGG_A
Cuts at: 1595 1595
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2404 2404
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 4546 4578 114
Size: 32 4400 32 943
Fragments arranged by size:
4400 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 1830 2050 1830
Size: 220 5187
PsiI TTA'TAA
Cuts at: 527 527
Size: 5407
PspOMI G'GGCC_C
Cuts at: 2303 2303
Size: 5407
PstI C_TGCA'G
Cuts at: 657 2452 3137 657
Size: 1795 685 2927
Fragments arranged by size:
2927 1795 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5407
PvuII CAG'CTG
Cuts at: 2830 3190 2830
Size: 360 5047
RsrII CG'GwC_CG
Cuts at: 3600 3600
Size: 5407
SacI G_AGCT'C
Cuts at: 818 2419 818
Size: 1601 3806
SacII CC_GC'GG
Cuts at: 166 196 428 166
Size: 30 232 5145
Fragments arranged by size:
5145 232 30
SalI G'TCGA_C
Cuts at: 1548 2442 1548
Size: 894 4513
SapI GCTCTTCn'nnn_
Cuts at: 905 1580 3428 3638 905
Size: 675 1848 210 2674
Fragments arranged by size:
2674 1848 675 210
SbfI CC_TGCA'GG
Cuts at: 2452 2452
Size: 5407
ScaI AGT'ACT
Cuts at: 2362 4924 2362
Size: 2562 2845
SfcI C'TryA_G
Cuts at: 33 653 2448 3133 4128 4319 33
Size: 620 1795 685 995 191 1121
Fragments arranged by size:
1795 1121 995 685 620 191
SfoI GGC'GCC
Cuts at: 408 422 2837 3085 408
Size: 14 2415 248 2730
Fragments arranged by size:
2730 2415 248 14
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5407
SmaI CCC'GGG
Cuts at: 204 235 2427 204
Size: 31 2192 3184
Fragments arranged by size:
3184 2192 31
SmlI C'TyrA_G
Cuts at: 859 982 1762 2560 3475 3969 4231 859
Size: 123 780 798 915 494 262 2035
Fragments arranged by size:
2035 915 798 780 494 262 123
SnaBI TAC'GTA
Cuts at: 2397 2397
Size: 5407
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5407
SphI G_CATG'C
Cuts at: 2458 2958 2458
Size: 500 4907
SspI AAT'ATT
Cuts at: 1244 1244
Size: 5407
StuI AGG'CCT
Cuts at: 665 665
Size: 5407
StyI C'CwwG_G
Cuts at: 125 2219 2539 2893 125
Size: 2094 320 354 2639
Fragments arranged by size:
2639 2094 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 695 2988 3121 3580 3791 695
Size: 2293 133 459 211 2311
Fragments arranged by size:
2311 2293 459 211 133
TatI w'GTAC_w
Cuts at: 269 1004 2360 4922 269
Size: 735 1356 2562 754
Fragments arranged by size:
2562 1356 754 735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 1214 1246 2370 2402 4924 4956 283
Size: 32 899 32 1124 32 2522 32 734
Fragments arranged by size:
2522 1124 899 734 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3202 3202
Size: 5407
XbaI T'CTAG_A
Cuts at: 2436 2436
Size: 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2199 2900 2199
Size: 701 4706
XmaI C'CCGG_G
Cuts at: 202 233 2425 202
Size: 31 2192 3184
Fragments arranged by size:
3184 2192 31
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BmgBI BmtI BsiWI BstEII BstZ17I
Bsu36I CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI PacI PasI PmlI PshAI PspXI
PsrI SanDI SexAI SfiI SgrAI SrfI SwaI XhoI
XmnI ZraI