Restriction map for
pF1KSDA1142
(Circular) (Six-base) MAPSORT of: pF1KSDA1142.seq Check: 786 from: 1 to: 4451
pF1KSDA1142 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 287 1154 1489 287
Size: 867 335 3249
Fragments arranged by size:
3249 867 335
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 1321 1387 2276 2708 3455 3723 1291
Size: 30 66 889 432 747 268 2019
Fragments arranged by size:
2019 889 747 432 268 66 30
AflIII A'CryG_T
Cuts at: 1803 2907 3884 1803
Size: 1104 977 2370
Fragments arranged by size:
2370 1104 977
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AhdI GACnn_n'nnGTC
Cuts at: 1161 1161
Size: 4451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 249 948 1756 3323 249
Size: 699 808 1567 1377
Fragments arranged by size:
1567 1377 808 699
ApaI G_GGCC'C
Cuts at: 324 524 324
Size: 200 4251
ApaLI G'TGCA_C
Cuts at: 187 3221 187
Size: 3034 1417
ApoI r'AATT_y
Cuts at: 1455 3804 4060 1455
Size: 2349 256 1846
Fragments arranged by size:
2349 1846 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 316 419 425 693 1471 316
Size: 103 6 268 778 3296
Fragments arranged by size:
3296 778 268 103 6
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 430 868 1467 1881 2129 2164 430
Size: 438 599 414 248 35 2717
Fragments arranged by size:
2717 599 438 414 248 35
BanII G_rGCy'C
Cuts at: 324 426 473 524 658 839 1124 1205 1465
Size: 102 47 51 134 181 285 81 260
Cuts at: 1465 2495 324
Size: 1030 2280
Fragments arranged by size:
2280 1030 285 260 181 134 102 81
51 47
BbeI G_GCGC'C
Cuts at: 872 1885 2133 872
Size: 1013 248 3190
Fragments arranged by size:
3190 1013 248
BciVI GTATCCnnnnn_n'
Cuts at: 573 2349 3110 4451 573
Size: 1776 761 1341 573
Fragments arranged by size:
1776 1341 761 573
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglI GCCn_nnn'nGGC
Cuts at: 1345 1345
Size: 4451
BglII A'GATC_T
Cuts at: 155 1966 4003 155
Size: 1811 2037 603
Fragments arranged by size:
2037 1811 603
BlpI GC'TnA_GC
Cuts at: 1563 1563
Size: 4451
Bme1580I G_kGCm'C
Cuts at: 191 324 435 524 1076 1415 2076 2169 3225
Size: 133 111 89 552 339 661 93 1056
Cuts at: 3225 191
Size: 1417
Fragments arranged by size:
1417 1056 661 552 339 133 111 93
89
BmgBI CAC'GTC
Cuts at: 345 345
Size: 4451
BmrI ACTGGGnnnn_n'
Cuts at: 381 1663 1744 2079 4223 381
Size: 1282 81 335 2144 609
Fragments arranged by size:
2144 1282 609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 889 1143 2818 889
Size: 254 1675 2522
Fragments arranged by size:
2522 1675 254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 470 464
Size: 6 4445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 2506 2998 3296 1627
Size: 879 492 298 2782
Fragments arranged by size:
2782 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1325 4155 1325
Size: 2830 1621
BsaAI yAC'GTr
Cuts at: 1443 1804 1443
Size: 361 4090
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 869 971 1882 2130 2832 4328 869
Size: 102 911 248 702 1496 992
Fragments arranged by size:
1496 992 911 702 248 102
BsaWI w'CCGG_w
Cuts at: 1640 2161 2839 3113 3260 3671 1640
Size: 521 678 274 147 411 2420
Fragments arranged by size:
2420 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 604 634 604
Size: 30 4421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 384 387 390 393 922 271
Size: 113 3 3 3 529 3800
Fragments arranged by size:
3800 529 113 3 3 3
BseYI C'CCAG_C
Cuts at: 436 450 473 1167 1340 1876 2634 3211 3707
Size: 14 23 694 173 536 758 577 496
Cuts at: 3707 436
Size: 1180
Fragments arranged by size:
1180 758 694 577 536 496 173 23
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1264 477
Size: 787 3664
BsiEI CG_ry'CG
Cuts at: 104 923 1993 2039 3247 104
Size: 819 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 819 46
BsiHKAI G_wGCw'C
Cuts at: 182 191 839 959 1124 1465 2243 2433 3225
Size: 9 648 120 165 341 778 190 792
Cuts at: 3225 182
Size: 1408
Fragments arranged by size:
1408 792 778 648 341 190 165 120
9
Bsp1286I G_dGCh'C
Cuts at: 182 191 324 426 435 473 524 658 839
Size: 9 133 102 9 38 51 134 181
Cuts at: 839 959 1076 1124 1205 1415 1465 2076 2169
Size: 120 117 48 81 210 50 611 93
Cuts at: 2169 2243 2433 2495 3225 182
Size: 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 210 190 181 134 133
120 117 102 93 81 74 62 51
50 48 38 9 9
BspEI T'CCGG_A
Cuts at: 3671 3671
Size: 4451
BspHI T'CATG_A
Cuts at: 339 773 3627 339
Size: 434 2854 1163
Fragments arranged by size:
2854 1163 434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 734 1265 1286 1501 1715 2017 2398 734
Size: 531 21 215 214 302 381 2787
Fragments arranged by size:
2787 531 381 302 215 214 21
BsrBI CCG'CTC
Cuts at: 50 536 620 2743 2797 4281 4446 50
Size: 486 84 2123 54 1484 165 55
Fragments arranged by size:
2123 1484 486 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 2363 2363
Size: 4451
BsrFI r'CCGG_y
Cuts at: 269 2449 2630 2839 269
Size: 2180 181 209 1881
Fragments arranged by size:
2180 1881 209 181
BsrGI T'GTAC_A
Cuts at: 809 809
Size: 4451
BssSI C'ACGA_G
Cuts at: 208 790 2722 3080 208
Size: 582 1932 358 1579
Fragments arranged by size:
1932 1579 582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 186 671 1828 186
Size: 485 1157 2809
Fragments arranged by size:
2809 1157 485
BstBI TT'CG_AA
Cuts at: 110 2812 110
Size: 2702 1749
BstYI r'GATC_y
Cuts at: 1 155 1476 1943 1966 2301 2547 3559 3645
Size: 154 1321 467 23 335 246 1012 86
Cuts at: 3645 3657 4003 1
Size: 12 346 449
Fragments arranged by size:
1321 1012 467 449 346 335 246 154
86 23 12
BtgI C'CryG_G
Cuts at: 659 1349 659
Size: 690 3761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 660 2401 2582 2745 660
Size: 1741 181 163 2366
Fragments arranged by size:
2366 1741 181 163
BtsI GCAGTG_nn'
Cuts at: 941 1694 941
Size: 753 3698
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DraIII CAC_nnn'GTG
Cuts at: 186 1287 186
Size: 1101 3350
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 377 707 920 1382 2036 2210 2601 2628 3973
Size: 330 213 462 654 174 391 27 1345
Cuts at: 3973 377
Size: 855
Fragments arranged by size:
1345 855 654 462 391 330 213 174
27
EagI C'GGCC_G
Cuts at: 920 2036 920
Size: 1116 3335
EarI CTCTTCn'nnn_
Cuts at: 130 760 2474 2684 130
Size: 630 1714 210 1897
Fragments arranged by size:
1897 1714 630 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 2969 3115 4261 2659
Size: 310 146 1146 2849
Fragments arranged by size:
2849 1146 310 146
EcoICRI GAG'CTC
Cuts at: 837 1122 1463 837
Size: 285 341 3825
Fragments arranged by size:
3825 341 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 470 1291 1321 1387 2276 2708 3455 3723
Size: 6 821 30 66 889 432 747 268
Cuts at: 3723 464
Size: 1192
Fragments arranged by size:
1192 889 821 747 432 268 66 30
6
EcoNI CCTnn'n_nnAGG
Cuts at: 253 1282 1384 253
Size: 1029 102 3320
Fragments arranged by size:
3320 1029 102
EcoO109I rG'GnC_Cy
Cuts at: 320 321 372 454 521 945 1590 320
Size: 1 51 82 67 424 645 3181
Fragments arranged by size:
3181 645 424 82 67 51 1
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4451
FspI TGC'GCA
Cuts at: 688 730 2232 688
Size: 42 1502 2907
Fragments arranged by size:
2907 1502 42
HaeII r_GCGC'y
Cuts at: 872 1885 2133 3155 4134 872
Size: 1013 248 1022 979 1189
Fragments arranged by size:
1189 1022 1013 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 1147 1179 2293 2323 2325 2355 604
Size: 32 511 32 1114 30 2 30 2700
Fragments arranged by size:
2700 1114 511 32 32 30 30 2
HincII GTy'rAC
Cuts at: 288 1490 288
Size: 1202 3249
KasI G'GCGC_C
Cuts at: 868 1881 2129 868
Size: 1013 248 3190
Fragments arranged by size:
3190 1013 248
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3122 3306 194
Size: 2928 184 1339
Fragments arranged by size:
2928 1339 184
MscI TGG'CCA
Cuts at: 379 1384 2212 379
Size: 1005 828 2618
Fragments arranged by size:
2618 1005 828
MslI CAynn'nnrTG
Cuts at: 794 898 1240 2440 2567 794
Size: 104 342 1200 127 2678
Fragments arranged by size:
2678 1200 342 127 104
MspA1I CmG'CkG
Cuts at: 367 477 546 594 1344 1562 1876 2236 3249
Size: 110 69 48 750 218 314 360 1013
Cuts at: 3249 3494 367
Size: 245 1324
Fragments arranged by size:
1324 1013 750 360 314 245 218 110
69 48
NaeI GCC'GGC
Cuts at: 271 2632 271
Size: 2361 2090
NarI GG'CG_CC
Cuts at: 869 1882 2130 869
Size: 1013 248 3190
Fragments arranged by size:
3190 1013 248
NgoMIV G'CCGG_C
Cuts at: 269 2630 269
Size: 2361 2090
NspI r_CATG'y
Cuts at: 1504 2004 2911 1504
Size: 500 907 3044
Fragments arranged by size:
3044 907 500
PasI CC'CwG_GG
Cuts at: 966 966
Size: 4451
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PfoI T'CCnGG_A
Cuts at: 857 1310 857
Size: 453 3998
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 202 234 3590 3622 202
Size: 32 3356 32 1031
Fragments arranged by size:
3356 1031 32 32
PpuMI rG'GwC_Cy
Cuts at: 454 454
Size: 4451
PspOMI G'GGCC_C
Cuts at: 320 520 320
Size: 200 4251
PstI C_TGCA'G
Cuts at: 593 944 1498 2183 593
Size: 351 554 685 2861
Fragments arranged by size:
2861 685 554 351
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 477 594 1876 2236 477
Size: 117 1282 360 2692
Fragments arranged by size:
2692 1282 360 117
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 839 1124 1465 839
Size: 285 341 3825
Fragments arranged by size:
3825 341 285
SalI G'TCGA_C
Cuts at: 286 1488 286
Size: 1202 3249
SanDI GG'GwC_CC
Cuts at: 454 454
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 760 2474 2684 760
Size: 1714 210 2527
Fragments arranged by size:
2527 1714 210
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SexAI A'CCwGG_T
Cuts at: 821 821
Size: 4451
SfcI C'TryA_G
Cuts at: 33 589 940 1494 2179 3172 3363 33
Size: 556 351 554 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 554 351 191
SfoI GGC'GCC
Cuts at: 870 1883 2131 870
Size: 1013 248 3190
Fragments arranged by size:
3190 1013 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 318 1473 318
Size: 1155 3296
SmlI C'TyrA_G
Cuts at: 1606 2521 3013 3275 1606
Size: 915 492 262 2782
Fragments arranged by size:
2782 915 492 262
SnaBI TAC'GTA
Cuts at: 1443 1443
Size: 4451
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StyI C'CwwG_G
Cuts at: 326 1385 1585 1939 326
Size: 1059 200 354 2838
Fragments arranged by size:
2838 1059 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 831 904 1388 2034 2167 2626 2835 831
Size: 73 484 646 133 459 209 2447
Fragments arranged by size:
2447 646 484 459 209 133 73
TatI w'GTAC_w
Cuts at: 809 3966 809
Size: 3157 1294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4419
Tth111I GACn'n_nGTC
Cuts at: 883 2248 883
Size: 1365 3086
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XmaI C'CCGG_G
Cuts at: 316 1471 316
Size: 1155 3296
XmnI GAAnn'nnTTC
Cuts at: 1306 1306
Size: 4451
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI