Restriction map for pF1KSDA1169
 (Circular) (Six-base) MAPSORT of: pF1KSDA1169.seq  Check: 7436  from: 1  to: 5585



pF1KSDA1169  5585 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AccI GT'mk_AC

Cuts at:   2623    2623 
   Size:       5585
 
Acc65I G'GTAC_C

Cuts at:   2601    2601 
   Size:       5585
 
AclI AA'CG_TT

Cuts at:   5425    5425 
   Size:       5585
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    884     897    1621    2455    3410    3842    4589    4857     884 
   Size:         13     724     834     955     432     747     268    1612

  Fragments arranged by size: 

                1612     955     834     747     724     432     268      13
            
 
AfeI AGC'GCT

Cuts at:   1261    1780    2557    1261 
   Size:        519     777    4289

  Fragments arranged by size: 

                4289     777     519
 
AflIII A'CryG_T

Cuts at:   2937    4041    5018    2937 
   Size:       1104     977    3504

  Fragments arranged by size: 

                3504    1104     977
 
AgeI A'CCGG_T

Cuts at:   3973    3973 
   Size:       5585
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    756     756 
   Size:       5585
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    994    1026    1522    1554    1789    1821     994 
   Size:         32     496      32     235      32    4758

  Fragments arranged by size: 

                4758     496     235      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    810    1755    2890    4457     810 
   Size:        945    1135    1567    1938

  Fragments arranged by size: 

                1938    1567    1135     945
 
ApaI G_GGCC'C

Cuts at:   1621    1621 
   Size:       5585
 
ApaLI G'TGCA_C

Cuts at:   4355    4355 
   Size:       5585
 
ApoI r'AATT_y

Cuts at:   2589    4938    5194    2589 
   Size:       2349     256    2980

  Fragments arranged by size: 

                2980    2349     256
 
AseI AT'TA_AT

Cuts at:     20    1427      20 
   Size:       1407    4178
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5585
 
AvaI C'yCGr_G

Cuts at:    728    2185    2605     728 
   Size:       1457     420    3708

  Fragments arranged by size: 

                3708    1457     420
 
BamHI G'GATC_C

Cuts at:   1496    2610    1496 
   Size:       1114    4471
 
BanI G'GyrC_C

Cuts at:   2601    3015    3263    3298    2601 
   Size:        414     248      35    4888

  Fragments arranged by size: 

                4888     414     248      35
 
BanII G_rGCy'C

Cuts at:   1621    1718    2054    2303    2599    3629    1621 
   Size:         97     336     249     296    1030    3577

  Fragments arranged by size: 

                3577    1030     336     296     249      97
 
BbeI G_GCGC'C

Cuts at:   3019    3267    3019 
   Size:        248    5337
 
BbsI GAAGACnn'nnnn_

Cuts at:   1011    1628    1896    2190    1011 
   Size:        617     268     294    4406

  Fragments arranged by size: 

                4406     617     294     268
 
BbvCI CC'TCA_GC

Cuts at:   1756    1756 
   Size:       5585
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1324    1358    1324 
   Size:         34    5551
 
BciVI GTATCCnnnnn_n'

Cuts at:   3483    4244    5585    3483 
   Size:        761    1341    3483

  Fragments arranged by size: 

                3483    1341     761
 
BclI T'GATC_A

Cuts at:    854    3105     854 
   Size:       2251    3334
 
BglI GCCn_nnn'nGGC

Cuts at:    193     193 
   Size:       5585
 
BglII A'GATC_T

Cuts at:    797    3100    5137     797 
   Size:       2303    2037    1245

  Fragments arranged by size: 

                2303    2037    1245
 
BlpI GC'TnA_GC

Cuts at:   1306    2697    1306 
   Size:       1391    4194
 
Bme1580I G_kGCm'C

Cuts at:    189    1553    1621    3210    3303    4359     189 
   Size:       1364      68    1589      93    1056    1415

  Fragments arranged by size: 

                1589    1415    1364    1056      93      68
 
BmgBI CAC'GTC

Cuts at:    150     150 
   Size:       5585
 
BmrI ACTGGGnnnn_n'

Cuts at:    269     333    2797    2878    3213    5357     269 
   Size:         64    2464      81     335    2144     497

  Fragments arranged by size: 

                2464    2144     497     335      81      64
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2373    3952    2373 
   Size:       1579    4006
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    291     343     574     943    1575    1732    2133    2317    2368 
   Size:         52     231     369     632     157     401     184      51

Cuts at:   2368     291 
   Size:       3508

  Fragments arranged by size: 

                3508     632     401     369     231     184     157      52
                  51
 
Bpu10I CC'TnA_GC

Cuts at:   1756    2428    1756 
   Size:        672    4913
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2278    2761    3640    4132    4430    2278 
   Size:        483     879     492     298    3433

  Fragments arranged by size: 

                3433     879     492     483     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2344    5289    2344 
   Size:       2945    2640
 
BsaAI yAC'GTr

Cuts at:   1548    2577    2938    1548 
   Size:       1029     361    4195

  Fragments arranged by size: 

                4195    1029     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2164    3099    5136       6 
   Size:       2158     935    2037     455

  Fragments arranged by size: 

                2158    2037     935     455
 
BsaHI Gr'CG_yC

Cuts at:    776    2090    3016    3264    3966    5462     776 
   Size:       1314     926     248     702    1496     899

  Fragments arranged by size: 

                1496    1314     926     899     702     248
 
BsaWI w'CCGG_w

Cuts at:   1639    2774    3295    3973    4247    4394    4805    1639 
   Size:       1135     521     678     274     147     411    2419

  Fragments arranged by size: 

                2419    1135     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    871     901    1522    1552    1618    1648     871 
   Size:         30     621      30      66      30    4808

  Fragments arranged by size: 

                4808     621      66      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1197    1399    2316    2464    2485    1197 
   Size:        202     917     148      21    4297

  Fragments arranged by size: 

                4297     917     202     148      21
 
BseYI C'CCAG_C

Cuts at:    609    2531    3010    3768    4345    4841     609 
   Size:       1922     479     758     577     496    1353

  Fragments arranged by size: 

                1922    1353     758     577     496     479
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    691    1117    2537     691 
   Size:        426    1420    3739

  Fragments arranged by size: 

                3739    1420     426
 
BsiEI CG_ry'CG

Cuts at:    104    3127    3173    4381     104 
   Size:       3023      46    1208    1308

  Fragments arranged by size: 

                3023    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    735    1379    2054    2303    2599    3377    3567    4359     735 
   Size:        644     675     249     296     778     190     792    1961

  Fragments arranged by size: 

                1961     792     778     675     644     296     249     190
            
 
Bsp1286I G_dGCh'C

Cuts at:    189     735    1379    1553    1621    1718    2054    2303    2599 
   Size:        546     644     174      68      97     336     249     296

Cuts at:   2599    3210    3303    3377    3567    3629    4359     189 
   Size:        611      93      74     190      62     730    1415

  Fragments arranged by size: 

                1415     730     644     611     546     336     296     249
                 190     174      97      93      74      68      62
 
BspEI T'CCGG_A

Cuts at:   1639    4805    1639 
   Size:       3166    2419
 
BspHI T'CATG_A

Cuts at:    851    1058    2129    4761     851 
   Size:        207    1071    2632    1675

  Fragments arranged by size: 

                2632    1675    1071     207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    797    1073    2635    2849    3151    3532     797 
   Size:        276    1562     214     302     381    2850

  Fragments arranged by size: 

                2850    1562     381     302     276     214
 
BsrBI CCG'CTC

Cuts at:     50     156    3877    3931    5415    5580      50 
   Size:        106    3721      54    1484     165      55

  Fragments arranged by size: 

                3721    1484     165     106      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3497    3497 
   Size:       5585
 
BsrFI r'CCGG_y

Cuts at:   1192    1609    3583    3764    3973    1192 
   Size:        417    1974     181     209    2804

  Fragments arranged by size: 

                2804    1974     417     209     181
 
BssSI C'ACGA_G

Cuts at:   1501    1923    3856    4214    1501 
   Size:        422    1933     358    2872

  Fragments arranged by size: 

                2872    1933     422     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2962    2962 
   Size:       5585
 
BstBI TT'CG_AA

Cuts at:    110    3946     110 
   Size:       3836    1749
 
BstEII G'GTnAC_C

Cuts at:    723     723 
   Size:       5585
 
BstXI CCAn_nnnn'nTGG

Cuts at:    210     414    2346     210 
   Size:        204    1932    3449

  Fragments arranged by size: 

                3449    1932     204
 
BstYI r'GATC_y

Cuts at:      1     797    1496    2453    2610    3077    3100    3435    3681 
   Size:        796     699     957     157     467      23     335     246

Cuts at:   3681    4693    4779    4791    5137       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     957     796     699     467     449     346     335
                 246     157      86      23      12
 
BtgI C'CryG_G

Cuts at:    125     467     644    1964    1979    2150    2387     125 
   Size:        342     177    1320      15     171     237    3323

  Fragments arranged by size: 

                3323    1320     342     237     177     171      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3535    3716    3879    3535 
   Size:        181     163    5241

  Fragments arranged by size: 

                5241     181     163
 
BtsI GCAGTG_nn'

Cuts at:    189     733    2520    2828     189 
   Size:        544    1787     308    2946

  Fragments arranged by size: 

                2946    1787     544     308
 
DraI TTT'AAA

Cuts at:   2584    2584 
   Size:       5585
 
DraIII CAC_nnn'GTG

Cuts at:   1965    1965 
   Size:       5585
 
DrdI GACnn_nn'nnGTC

Cuts at:   3291    4149    3291 
   Size:        858    4727
 
EaeI y'GGCC_r

Cuts at:    464    1826    3170    3344    3735    3762    5107     464 
   Size:       1362    1344     174     391      27    1345     942

  Fragments arranged by size: 

                1362    1345    1344     942     391     174      27
 
EagI C'GGCC_G

Cuts at:   3170    3170 
   Size:       5585
 
EarI CTCTTCn'nnn_

Cuts at:    215     451    2273    3608    3818     215 
   Size:        236    1822    1335     210    1982

  Fragments arranged by size: 

                1982    1822    1335     236     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3793    4103    4249    5395    3793 
   Size:        310     146    1146    3983

  Fragments arranged by size: 

                3983    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2052    2301    2597    2052 
   Size:        249     296    5040

  Fragments arranged by size: 

                5040     296     249
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    291     343     574     884     897     943    1575    1621    1732 
   Size:         52     231     310      13      46     632      46     111

Cuts at:   1732    2133    2317    2368    2455    3410    3842    4589    4857 
   Size:        401     184      51      87     955     432     747     268

Cuts at:   4857     291 
   Size:       1019

  Fragments arranged by size: 

                1019     955     747     632     432     401     310     268
                 231     184     111      87      52      51      46      46
                  13
 
EcoNI CCTnn'n_nnAGG

Cuts at:    499    2260     499 
   Size:       1761    3824
 
EcoO109I rG'GnC_Cy

Cuts at:    270     504    1278    1618    2321    2724     270 
   Size:        234     774     340     703     403    3131

  Fragments arranged by size: 

                3131     774     703     403     340     234
 
EcoRI G'AATT_C

Cuts at:   2589    2589 
   Size:       5585
 
EcoRV GAT'ATC

Cuts at:   1329    1329 
   Size:       5585
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3604    3604 
   Size:       5585
 
FspI TGC'GCA

Cuts at:   3366    3366 
   Size:       5585
 
HaeII r_GCGC'y

Cuts at:    415    1263    1408    1699    1782    2559    3019    3267    4289 
   Size:        848     145     291      83     777     460     248    1022

Cuts at:   4289    5268     415 
   Size:        979     732

  Fragments arranged by size: 

                1022     979     848     777     732     460     291     248
                 145      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1618    1650    3427    3457    3459    3489    1618 
   Size:         32    1777      30       2      30    3714

  Fragments arranged by size: 

                3714    1777      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1485    2064    2624    1485 
   Size:        579     560    4446

  Fragments arranged by size: 

                4446     579     560
 
KasI G'GCGC_C

Cuts at:   3015    3263    3015 
   Size:        248    5337
 
KpnI G_GTAC'C

Cuts at:   2605    2605 
   Size:       5585
 
MluI A'CGCG_T

Cuts at:   5018    5018 
   Size:       5585
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    227    4256    4440     227 
   Size:       4029     184    1372

  Fragments arranged by size: 

                4029    1372     184
 
MscI TGG'CCA

Cuts at:    466    1828    3346     466 
   Size:       1362    1518    2705

  Fragments arranged by size: 

                2705    1518    1362
 
MslI CAynn'nnrTG

Cuts at:    130     691    2080    2155    3574    3701     130 
   Size:        561    1389      75    1419     127    2014

  Fragments arranged by size: 

                2014    1419    1389     561     127      75
 
MspA1I CmG'CkG

Cuts at:   1752    1949    2696    3010    3370    4383    4628    1752 
   Size:        197     747     314     360    1013     245    2709

  Fragments arranged by size: 

                2709    1013     747     360     314     245     197
 
NaeI GCC'GGC

Cuts at:   1194    1611    3766    1194 
   Size:        417    2155    3013

  Fragments arranged by size: 

                3013    2155     417
 
NarI GG'CG_CC

Cuts at:   3016    3264    3016 
   Size:        248    5337
 
NcoI C'CATG_G

Cuts at:    125     644    2150    2387     125 
   Size:        519    1506     237    3323

  Fragments arranged by size: 

                3323    1506     519     237
 
NgoMIV G'CCGG_C

Cuts at:   1192    1609    3764    1192 
   Size:        417    2155    3013

  Fragments arranged by size: 

                3013    2155     417
 
NspI r_CATG'y

Cuts at:   1159    2479    2638    3138    4045    1159 
   Size:       1320     159     500     907    2699

  Fragments arranged by size: 

                2699    1320     907     500     159
 
PciI A'CATG_T

Cuts at:   4041    4041 
   Size:       5585
 
PflMI CCAn_nnn'nTGG

Cuts at:   1965    1965 
   Size:       5585
 
PfoI T'CCnGG_A

Cuts at:    359     997    2294    2456     359 
   Size:        638    1297     162    3488

  Fragments arranged by size: 

                3488    1297     638     162
 
PmeI GTTT'AAAC

Cuts at:   2584    2584 
   Size:       5585
 
PmlI CAC'GTG

Cuts at:   1548    1548 
   Size:       5585
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1522    1554    2215    2247    4724    4756    1522 
   Size:         32     661      32    2477      32    2351

  Fragments arranged by size: 

                2477    2351     661      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:    646     646 
   Size:       5585
 
PspOMI G'GGCC_C

Cuts at:   1617    1617 
   Size:       5585
 
PspXI vC'TCGA_Gb

Cuts at:   2185    2185 
   Size:       5585
 
PstI C_TGCA'G

Cuts at:   2632    3317    2632 
   Size:        685    4900
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5585
 
PvuII CAG'CTG

Cuts at:   1752    3010    3370    1752 
   Size:       1258     360    3967

  Fragments arranged by size: 

                3967    1258     360
 
RsrII CG'GwC_CG

Cuts at:   3780    3780 
   Size:       5585
 
SacI G_AGCT'C

Cuts at:   2054    2303    2599    2054 
   Size:        249     296    5040

  Fragments arranged by size: 

                5040     296     249
 
SalI G'TCGA_C

Cuts at:   2622    2622 
   Size:       5585
 
SapI GCTCTTCn'nnn_

Cuts at:    215    2273    3608    3818     215 
   Size:       2058    1335     210    1982

  Fragments arranged by size: 

                2058    1982    1335     210
 
SbfI CC_TGCA'GG

Cuts at:   2632    2632 
   Size:       5585
 
ScaI AGT'ACT

Cuts at:   1630    5102    1630 
   Size:       3472    2113
 
SfcI C'TryA_G

Cuts at:     33    1203    2211    2628    3313    4306    4497      33 
   Size:       1170    1008     417     685     993     191    1121

  Fragments arranged by size: 

                1170    1121    1008     993     685     417     191
 
SfoI GGC'GCC

Cuts at:   3017    3265    3017 
   Size:        248    5337
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5585
 
SmaI CCC'GGG

Cuts at:   2607    2607 
   Size:       5585
 
SmlI C'TyrA_G

Cuts at:   2185    2257    2740    3655    4147    4409    2185 
   Size:         72     483     915     492     262    3361

  Fragments arranged by size: 

                3361     915     492     483     262      72
 
SnaBI TAC'GTA

Cuts at:   2577    2577 
   Size:       5585
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5585
 
SphI G_CATG'C

Cuts at:   1159    2479    2638    3138    1159 
   Size:       1320     159     500    3606

  Fragments arranged by size: 

                3606    1320     500     159
 
StuI AGG'CCT

Cuts at:   1188    1220    1450    2191    1188 
   Size:         32     230     741    4582

  Fragments arranged by size: 

                4582     741     230      32
 
StyI C'CwwG_G

Cuts at:    125     167     398     407     644    1445    2150    2387    2719 
   Size:         42     231       9     237     801     705     237     332

Cuts at:   2719    3073     125 
   Size:        354    2637

  Fragments arranged by size: 

                2637     801     705     354     332     237     237     231
                  42       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    406    3168    3301    3760    3969     406 
   Size:       2762     133     459     209    2022

  Fragments arranged by size: 

                2762    2022     459     209     133
 
TatI w'GTAC_w

Cuts at:   1466    1628    1927    5100    1466 
   Size:        162     299    3173    1951

  Fragments arranged by size: 

                3173    1951     299     162
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5102    5134    5102 
   Size:         32    5553
 
Tth111I GACn'n_nGTC

Cuts at:   3382    3382 
   Size:       5585
 
XbaI T'CTAG_A

Cuts at:   2616    2616 
   Size:       5585
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    693    1878    2280    2394    3080     693 
   Size:       1185     402     114     686    3198

  Fragments arranged by size: 

                3198    1185     686     402     114
 
XhoI C'TCGA_G

Cuts at:   2185    2185 
   Size:       5585
 
XmaI C'CCGG_G

Cuts at:   2605    2605 
   Size:       5585
 
XmnI GAAnn'nnTTC

Cuts at:   1534    1534 
   Size:       5585
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AscI    AvrII     BaeI
    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI   BssHII  BstZ17I
   Bsu36I     ClaI    CspCI     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PpuMI
     PsiI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI
     SspI     SwaI     ZraI
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