Restriction map for
pF1KSDA1169
(Circular) (Six-base) MAPSORT of: pF1KSDA1169.seq Check: 7436 from: 1 to: 5585
pF1KSDA1169 5585 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2623 2623
Size: 5585
Acc65I G'GTAC_C
Cuts at: 2601 2601
Size: 5585
AclI AA'CG_TT
Cuts at: 5425 5425
Size: 5585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 897 1621 2455 3410 3842 4589 4857 884
Size: 13 724 834 955 432 747 268 1612
Fragments arranged by size:
1612 955 834 747 724 432 268 13
AfeI AGC'GCT
Cuts at: 1261 1780 2557 1261
Size: 519 777 4289
Fragments arranged by size:
4289 777 519
AflIII A'CryG_T
Cuts at: 2937 4041 5018 2937
Size: 1104 977 3504
Fragments arranged by size:
3504 1104 977
AgeI A'CCGG_T
Cuts at: 3973 3973
Size: 5585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 756 756
Size: 5585
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 1522 1554 1789 1821 994
Size: 32 496 32 235 32 4758
Fragments arranged by size:
4758 496 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 810 1755 2890 4457 810
Size: 945 1135 1567 1938
Fragments arranged by size:
1938 1567 1135 945
ApaI G_GGCC'C
Cuts at: 1621 1621
Size: 5585
ApaLI G'TGCA_C
Cuts at: 4355 4355
Size: 5585
ApoI r'AATT_y
Cuts at: 2589 4938 5194 2589
Size: 2349 256 2980
Fragments arranged by size:
2980 2349 256
AseI AT'TA_AT
Cuts at: 20 1427 20
Size: 1407 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
AvaI C'yCGr_G
Cuts at: 728 2185 2605 728
Size: 1457 420 3708
Fragments arranged by size:
3708 1457 420
BamHI G'GATC_C
Cuts at: 1496 2610 1496
Size: 1114 4471
BanI G'GyrC_C
Cuts at: 2601 3015 3263 3298 2601
Size: 414 248 35 4888
Fragments arranged by size:
4888 414 248 35
BanII G_rGCy'C
Cuts at: 1621 1718 2054 2303 2599 3629 1621
Size: 97 336 249 296 1030 3577
Fragments arranged by size:
3577 1030 336 296 249 97
BbeI G_GCGC'C
Cuts at: 3019 3267 3019
Size: 248 5337
BbsI GAAGACnn'nnnn_
Cuts at: 1011 1628 1896 2190 1011
Size: 617 268 294 4406
Fragments arranged by size:
4406 617 294 268
BbvCI CC'TCA_GC
Cuts at: 1756 1756
Size: 5585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1324 1358 1324
Size: 34 5551
BciVI GTATCCnnnnn_n'
Cuts at: 3483 4244 5585 3483
Size: 761 1341 3483
Fragments arranged by size:
3483 1341 761
BclI T'GATC_A
Cuts at: 854 3105 854
Size: 2251 3334
BglI GCCn_nnn'nGGC
Cuts at: 193 193
Size: 5585
BglII A'GATC_T
Cuts at: 797 3100 5137 797
Size: 2303 2037 1245
Fragments arranged by size:
2303 2037 1245
BlpI GC'TnA_GC
Cuts at: 1306 2697 1306
Size: 1391 4194
Bme1580I G_kGCm'C
Cuts at: 189 1553 1621 3210 3303 4359 189
Size: 1364 68 1589 93 1056 1415
Fragments arranged by size:
1589 1415 1364 1056 93 68
BmgBI CAC'GTC
Cuts at: 150 150
Size: 5585
BmrI ACTGGGnnnn_n'
Cuts at: 269 333 2797 2878 3213 5357 269
Size: 64 2464 81 335 2144 497
Fragments arranged by size:
2464 2144 497 335 81 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2373 3952 2373
Size: 1579 4006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 343 574 943 1575 1732 2133 2317 2368
Size: 52 231 369 632 157 401 184 51
Cuts at: 2368 291
Size: 3508
Fragments arranged by size:
3508 632 401 369 231 184 157 52
51
Bpu10I CC'TnA_GC
Cuts at: 1756 2428 1756
Size: 672 4913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 2761 3640 4132 4430 2278
Size: 483 879 492 298 3433
Fragments arranged by size:
3433 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 2344 5289 2344
Size: 2945 2640
BsaAI yAC'GTr
Cuts at: 1548 2577 2938 1548
Size: 1029 361 4195
Fragments arranged by size:
4195 1029 361
BsaBI GATnn'nnATC
Cuts at: 6 2164 3099 5136 6
Size: 2158 935 2037 455
Fragments arranged by size:
2158 2037 935 455
BsaHI Gr'CG_yC
Cuts at: 776 2090 3016 3264 3966 5462 776
Size: 1314 926 248 702 1496 899
Fragments arranged by size:
1496 1314 926 899 702 248
BsaWI w'CCGG_w
Cuts at: 1639 2774 3295 3973 4247 4394 4805 1639
Size: 1135 521 678 274 147 411 2419
Fragments arranged by size:
2419 1135 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 871 901 1522 1552 1618 1648 871
Size: 30 621 30 66 30 4808
Fragments arranged by size:
4808 621 66 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1197 1399 2316 2464 2485 1197
Size: 202 917 148 21 4297
Fragments arranged by size:
4297 917 202 148 21
BseYI C'CCAG_C
Cuts at: 609 2531 3010 3768 4345 4841 609
Size: 1922 479 758 577 496 1353
Fragments arranged by size:
1922 1353 758 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1117 2537 691
Size: 426 1420 3739
Fragments arranged by size:
3739 1420 426
BsiEI CG_ry'CG
Cuts at: 104 3127 3173 4381 104
Size: 3023 46 1208 1308
Fragments arranged by size:
3023 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 735 1379 2054 2303 2599 3377 3567 4359 735
Size: 644 675 249 296 778 190 792 1961
Fragments arranged by size:
1961 792 778 675 644 296 249 190
Bsp1286I G_dGCh'C
Cuts at: 189 735 1379 1553 1621 1718 2054 2303 2599
Size: 546 644 174 68 97 336 249 296
Cuts at: 2599 3210 3303 3377 3567 3629 4359 189
Size: 611 93 74 190 62 730 1415
Fragments arranged by size:
1415 730 644 611 546 336 296 249
190 174 97 93 74 68 62
BspEI T'CCGG_A
Cuts at: 1639 4805 1639
Size: 3166 2419
BspHI T'CATG_A
Cuts at: 851 1058 2129 4761 851
Size: 207 1071 2632 1675
Fragments arranged by size:
2632 1675 1071 207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 797 1073 2635 2849 3151 3532 797
Size: 276 1562 214 302 381 2850
Fragments arranged by size:
2850 1562 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 156 3877 3931 5415 5580 50
Size: 106 3721 54 1484 165 55
Fragments arranged by size:
3721 1484 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 3497 3497
Size: 5585
BsrFI r'CCGG_y
Cuts at: 1192 1609 3583 3764 3973 1192
Size: 417 1974 181 209 2804
Fragments arranged by size:
2804 1974 417 209 181
BssSI C'ACGA_G
Cuts at: 1501 1923 3856 4214 1501
Size: 422 1933 358 2872
Fragments arranged by size:
2872 1933 422 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2962 2962
Size: 5585
BstBI TT'CG_AA
Cuts at: 110 3946 110
Size: 3836 1749
BstEII G'GTnAC_C
Cuts at: 723 723
Size: 5585
BstXI CCAn_nnnn'nTGG
Cuts at: 210 414 2346 210
Size: 204 1932 3449
Fragments arranged by size:
3449 1932 204
BstYI r'GATC_y
Cuts at: 1 797 1496 2453 2610 3077 3100 3435 3681
Size: 796 699 957 157 467 23 335 246
Cuts at: 3681 4693 4779 4791 5137 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 957 796 699 467 449 346 335
246 157 86 23 12
BtgI C'CryG_G
Cuts at: 125 467 644 1964 1979 2150 2387 125
Size: 342 177 1320 15 171 237 3323
Fragments arranged by size:
3323 1320 342 237 177 171 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3535 3716 3879 3535
Size: 181 163 5241
Fragments arranged by size:
5241 181 163
BtsI GCAGTG_nn'
Cuts at: 189 733 2520 2828 189
Size: 544 1787 308 2946
Fragments arranged by size:
2946 1787 544 308
DraI TTT'AAA
Cuts at: 2584 2584
Size: 5585
DraIII CAC_nnn'GTG
Cuts at: 1965 1965
Size: 5585
DrdI GACnn_nn'nnGTC
Cuts at: 3291 4149 3291
Size: 858 4727
EaeI y'GGCC_r
Cuts at: 464 1826 3170 3344 3735 3762 5107 464
Size: 1362 1344 174 391 27 1345 942
Fragments arranged by size:
1362 1345 1344 942 391 174 27
EagI C'GGCC_G
Cuts at: 3170 3170
Size: 5585
EarI CTCTTCn'nnn_
Cuts at: 215 451 2273 3608 3818 215
Size: 236 1822 1335 210 1982
Fragments arranged by size:
1982 1822 1335 236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3793 4103 4249 5395 3793
Size: 310 146 1146 3983
Fragments arranged by size:
3983 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2052 2301 2597 2052
Size: 249 296 5040
Fragments arranged by size:
5040 296 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 343 574 884 897 943 1575 1621 1732
Size: 52 231 310 13 46 632 46 111
Cuts at: 1732 2133 2317 2368 2455 3410 3842 4589 4857
Size: 401 184 51 87 955 432 747 268
Cuts at: 4857 291
Size: 1019
Fragments arranged by size:
1019 955 747 632 432 401 310 268
231 184 111 87 52 51 46 46
13
EcoNI CCTnn'n_nnAGG
Cuts at: 499 2260 499
Size: 1761 3824
EcoO109I rG'GnC_Cy
Cuts at: 270 504 1278 1618 2321 2724 270
Size: 234 774 340 703 403 3131
Fragments arranged by size:
3131 774 703 403 340 234
EcoRI G'AATT_C
Cuts at: 2589 2589
Size: 5585
EcoRV GAT'ATC
Cuts at: 1329 1329
Size: 5585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 5585
FspI TGC'GCA
Cuts at: 3366 3366
Size: 5585
HaeII r_GCGC'y
Cuts at: 415 1263 1408 1699 1782 2559 3019 3267 4289
Size: 848 145 291 83 777 460 248 1022
Cuts at: 4289 5268 415
Size: 979 732
Fragments arranged by size:
1022 979 848 777 732 460 291 248
145 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1650 3427 3457 3459 3489 1618
Size: 32 1777 30 2 30 3714
Fragments arranged by size:
3714 1777 32 30 30 2
HincII GTy'rAC
Cuts at: 1485 2064 2624 1485
Size: 579 560 4446
Fragments arranged by size:
4446 579 560
KasI G'GCGC_C
Cuts at: 3015 3263 3015
Size: 248 5337
KpnI G_GTAC'C
Cuts at: 2605 2605
Size: 5585
MluI A'CGCG_T
Cuts at: 5018 5018
Size: 5585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 4256 4440 227
Size: 4029 184 1372
Fragments arranged by size:
4029 1372 184
MscI TGG'CCA
Cuts at: 466 1828 3346 466
Size: 1362 1518 2705
Fragments arranged by size:
2705 1518 1362
MslI CAynn'nnrTG
Cuts at: 130 691 2080 2155 3574 3701 130
Size: 561 1389 75 1419 127 2014
Fragments arranged by size:
2014 1419 1389 561 127 75
MspA1I CmG'CkG
Cuts at: 1752 1949 2696 3010 3370 4383 4628 1752
Size: 197 747 314 360 1013 245 2709
Fragments arranged by size:
2709 1013 747 360 314 245 197
NaeI GCC'GGC
Cuts at: 1194 1611 3766 1194
Size: 417 2155 3013
Fragments arranged by size:
3013 2155 417
NarI GG'CG_CC
Cuts at: 3016 3264 3016
Size: 248 5337
NcoI C'CATG_G
Cuts at: 125 644 2150 2387 125
Size: 519 1506 237 3323
Fragments arranged by size:
3323 1506 519 237
NgoMIV G'CCGG_C
Cuts at: 1192 1609 3764 1192
Size: 417 2155 3013
Fragments arranged by size:
3013 2155 417
NspI r_CATG'y
Cuts at: 1159 2479 2638 3138 4045 1159
Size: 1320 159 500 907 2699
Fragments arranged by size:
2699 1320 907 500 159
PciI A'CATG_T
Cuts at: 4041 4041
Size: 5585
PflMI CCAn_nnn'nTGG
Cuts at: 1965 1965
Size: 5585
PfoI T'CCnGG_A
Cuts at: 359 997 2294 2456 359
Size: 638 1297 162 3488
Fragments arranged by size:
3488 1297 638 162
PmeI GTTT'AAAC
Cuts at: 2584 2584
Size: 5585
PmlI CAC'GTG
Cuts at: 1548 1548
Size: 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1522 1554 2215 2247 4724 4756 1522
Size: 32 661 32 2477 32 2351
Fragments arranged by size:
2477 2351 661 32 32 32
PshAI GACnn'nnGTC
Cuts at: 646 646
Size: 5585
PspOMI G'GGCC_C
Cuts at: 1617 1617
Size: 5585
PspXI vC'TCGA_Gb
Cuts at: 2185 2185
Size: 5585
PstI C_TGCA'G
Cuts at: 2632 3317 2632
Size: 685 4900
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5585
PvuII CAG'CTG
Cuts at: 1752 3010 3370 1752
Size: 1258 360 3967
Fragments arranged by size:
3967 1258 360
RsrII CG'GwC_CG
Cuts at: 3780 3780
Size: 5585
SacI G_AGCT'C
Cuts at: 2054 2303 2599 2054
Size: 249 296 5040
Fragments arranged by size:
5040 296 249
SalI G'TCGA_C
Cuts at: 2622 2622
Size: 5585
SapI GCTCTTCn'nnn_
Cuts at: 215 2273 3608 3818 215
Size: 2058 1335 210 1982
Fragments arranged by size:
2058 1982 1335 210
SbfI CC_TGCA'GG
Cuts at: 2632 2632
Size: 5585
ScaI AGT'ACT
Cuts at: 1630 5102 1630
Size: 3472 2113
SfcI C'TryA_G
Cuts at: 33 1203 2211 2628 3313 4306 4497 33
Size: 1170 1008 417 685 993 191 1121
Fragments arranged by size:
1170 1121 1008 993 685 417 191
SfoI GGC'GCC
Cuts at: 3017 3265 3017
Size: 248 5337
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
SmaI CCC'GGG
Cuts at: 2607 2607
Size: 5585
SmlI C'TyrA_G
Cuts at: 2185 2257 2740 3655 4147 4409 2185
Size: 72 483 915 492 262 3361
Fragments arranged by size:
3361 915 492 483 262 72
SnaBI TAC'GTA
Cuts at: 2577 2577
Size: 5585
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5585
SphI G_CATG'C
Cuts at: 1159 2479 2638 3138 1159
Size: 1320 159 500 3606
Fragments arranged by size:
3606 1320 500 159
StuI AGG'CCT
Cuts at: 1188 1220 1450 2191 1188
Size: 32 230 741 4582
Fragments arranged by size:
4582 741 230 32
StyI C'CwwG_G
Cuts at: 125 167 398 407 644 1445 2150 2387 2719
Size: 42 231 9 237 801 705 237 332
Cuts at: 2719 3073 125
Size: 354 2637
Fragments arranged by size:
2637 801 705 354 332 237 237 231
42 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 3168 3301 3760 3969 406
Size: 2762 133 459 209 2022
Fragments arranged by size:
2762 2022 459 209 133
TatI w'GTAC_w
Cuts at: 1466 1628 1927 5100 1466
Size: 162 299 3173 1951
Fragments arranged by size:
3173 1951 299 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5102 5134 5102
Size: 32 5553
Tth111I GACn'n_nGTC
Cuts at: 3382 3382
Size: 5585
XbaI T'CTAG_A
Cuts at: 2616 2616
Size: 5585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 1878 2280 2394 3080 693
Size: 1185 402 114 686 3198
Fragments arranged by size:
3198 1185 686 402 114
XhoI C'TCGA_G
Cuts at: 2185 2185
Size: 5585
XmaI C'CCGG_G
Cuts at: 2605 2605
Size: 5585
XmnI GAAnn'nnTTC
Cuts at: 1534 1534
Size: 5585
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI ZraI