(Circular) (Six-base) MAPSORT of: pF1KSDA1169.seq Check: 7436 from: 1 to: 5585 pF1KSDA1169 5585 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AccI GT'mk_AC Cuts at: 2623 2623 Size: 5585 Acc65I G'GTAC_C Cuts at: 2601 2601 Size: 5585 AclI AA'CG_TT Cuts at: 5425 5425 Size: 5585 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 884 897 1621 2455 3410 3842 4589 4857 884 Size: 13 724 834 955 432 747 268 1612 Fragments arranged by size: 1612 955 834 747 724 432 268 13 AfeI AGC'GCT Cuts at: 1261 1780 2557 1261 Size: 519 777 4289 Fragments arranged by size: 4289 777 519 AflIII A'CryG_T Cuts at: 2937 4041 5018 2937 Size: 1104 977 3504 Fragments arranged by size: 3504 1104 977 AgeI A'CCGG_T Cuts at: 3973 3973 Size: 5585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 756 756 Size: 5585 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 994 1026 1522 1554 1789 1821 994 Size: 32 496 32 235 32 4758 Fragments arranged by size: 4758 496 235 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 810 1755 2890 4457 810 Size: 945 1135 1567 1938 Fragments arranged by size: 1938 1567 1135 945 ApaI G_GGCC'C Cuts at: 1621 1621 Size: 5585 ApaLI G'TGCA_C Cuts at: 4355 4355 Size: 5585 ApoI r'AATT_y Cuts at: 2589 4938 5194 2589 Size: 2349 256 2980 Fragments arranged by size: 2980 2349 256 AseI AT'TA_AT Cuts at: 20 1427 20 Size: 1407 4178 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5585 AvaI C'yCGr_G Cuts at: 728 2185 2605 728 Size: 1457 420 3708 Fragments arranged by size: 3708 1457 420 BamHI G'GATC_C Cuts at: 1496 2610 1496 Size: 1114 4471 BanI G'GyrC_C Cuts at: 2601 3015 3263 3298 2601 Size: 414 248 35 4888 Fragments arranged by size: 4888 414 248 35 BanII G_rGCy'C Cuts at: 1621 1718 2054 2303 2599 3629 1621 Size: 97 336 249 296 1030 3577 Fragments arranged by size: 3577 1030 336 296 249 97 BbeI G_GCGC'C Cuts at: 3019 3267 3019 Size: 248 5337 BbsI GAAGACnn'nnnn_ Cuts at: 1011 1628 1896 2190 1011 Size: 617 268 294 4406 Fragments arranged by size: 4406 617 294 268 BbvCI CC'TCA_GC Cuts at: 1756 1756 Size: 5585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1324 1358 1324 Size: 34 5551 BciVI GTATCCnnnnn_n' Cuts at: 3483 4244 5585 3483 Size: 761 1341 3483 Fragments arranged by size: 3483 1341 761 BclI T'GATC_A Cuts at: 854 3105 854 Size: 2251 3334 BglI GCCn_nnn'nGGC Cuts at: 193 193 Size: 5585 BglII A'GATC_T Cuts at: 797 3100 5137 797 Size: 2303 2037 1245 Fragments arranged by size: 2303 2037 1245 BlpI GC'TnA_GC Cuts at: 1306 2697 1306 Size: 1391 4194 Bme1580I G_kGCm'C Cuts at: 189 1553 1621 3210 3303 4359 189 Size: 1364 68 1589 93 1056 1415 Fragments arranged by size: 1589 1415 1364 1056 93 68 BmgBI CAC'GTC Cuts at: 150 150 Size: 5585 BmrI ACTGGGnnnn_n' Cuts at: 269 333 2797 2878 3213 5357 269 Size: 64 2464 81 335 2144 497 Fragments arranged by size: 2464 2144 497 335 81 64 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2373 3952 2373 Size: 1579 4006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 291 343 574 943 1575 1732 2133 2317 2368 Size: 52 231 369 632 157 401 184 51 Cuts at: 2368 291 Size: 3508 Fragments arranged by size: 3508 632 401 369 231 184 157 52 51 Bpu10I CC'TnA_GC Cuts at: 1756 2428 1756 Size: 672 4913 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2278 2761 3640 4132 4430 2278 Size: 483 879 492 298 3433 Fragments arranged by size: 3433 879 492 483 298 BsaI GGTCTCn'nnnn_ Cuts at: 2344 5289 2344 Size: 2945 2640 BsaAI yAC'GTr Cuts at: 1548 2577 2938 1548 Size: 1029 361 4195 Fragments arranged by size: 4195 1029 361 BsaBI GATnn'nnATC Cuts at: 6 2164 3099 5136 6 Size: 2158 935 2037 455 Fragments arranged by size: 2158 2037 935 455 BsaHI Gr'CG_yC Cuts at: 776 2090 3016 3264 3966 5462 776 Size: 1314 926 248 702 1496 899 Fragments arranged by size: 1496 1314 926 899 702 248 BsaWI w'CCGG_w Cuts at: 1639 2774 3295 3973 4247 4394 4805 1639 Size: 1135 521 678 274 147 411 2419 Fragments arranged by size: 2419 1135 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 871 901 1522 1552 1618 1648 871 Size: 30 621 30 66 30 4808 Fragments arranged by size: 4808 621 66 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1197 1399 2316 2464 2485 1197 Size: 202 917 148 21 4297 Fragments arranged by size: 4297 917 202 148 21 BseYI C'CCAG_C Cuts at: 609 2531 3010 3768 4345 4841 609 Size: 1922 479 758 577 496 1353 Fragments arranged by size: 1922 1353 758 577 496 479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 691 1117 2537 691 Size: 426 1420 3739 Fragments arranged by size: 3739 1420 426 BsiEI CG_ry'CG Cuts at: 104 3127 3173 4381 104 Size: 3023 46 1208 1308 Fragments arranged by size: 3023 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 735 1379 2054 2303 2599 3377 3567 4359 735 Size: 644 675 249 296 778 190 792 1961 Fragments arranged by size: 1961 792 778 675 644 296 249 190 Bsp1286I G_dGCh'C Cuts at: 189 735 1379 1553 1621 1718 2054 2303 2599 Size: 546 644 174 68 97 336 249 296 Cuts at: 2599 3210 3303 3377 3567 3629 4359 189 Size: 611 93 74 190 62 730 1415 Fragments arranged by size: 1415 730 644 611 546 336 296 249 190 174 97 93 74 68 62 BspEI T'CCGG_A Cuts at: 1639 4805 1639 Size: 3166 2419 BspHI T'CATG_A Cuts at: 851 1058 2129 4761 851 Size: 207 1071 2632 1675 Fragments arranged by size: 2632 1675 1071 207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 797 1073 2635 2849 3151 3532 797 Size: 276 1562 214 302 381 2850 Fragments arranged by size: 2850 1562 381 302 276 214 BsrBI CCG'CTC Cuts at: 50 156 3877 3931 5415 5580 50 Size: 106 3721 54 1484 165 55 Fragments arranged by size: 3721 1484 165 106 55 54 BsrDI GCAATG_nn' Cuts at: 3497 3497 Size: 5585 BsrFI r'CCGG_y Cuts at: 1192 1609 3583 3764 3973 1192 Size: 417 1974 181 209 2804 Fragments arranged by size: 2804 1974 417 209 181 BssSI C'ACGA_G Cuts at: 1501 1923 3856 4214 1501 Size: 422 1933 358 2872 Fragments arranged by size: 2872 1933 422 358 BstAPI GCAn_nnn'nTGC Cuts at: 2962 2962 Size: 5585 BstBI TT'CG_AA Cuts at: 110 3946 110 Size: 3836 1749 BstEII G'GTnAC_C Cuts at: 723 723 Size: 5585 BstXI CCAn_nnnn'nTGG Cuts at: 210 414 2346 210 Size: 204 1932 3449 Fragments arranged by size: 3449 1932 204 BstYI r'GATC_y Cuts at: 1 797 1496 2453 2610 3077 3100 3435 3681 Size: 796 699 957 157 467 23 335 246 Cuts at: 3681 4693 4779 4791 5137 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 957 796 699 467 449 346 335 246 157 86 23 12 BtgI C'CryG_G Cuts at: 125 467 644 1964 1979 2150 2387 125 Size: 342 177 1320 15 171 237 3323 Fragments arranged by size: 3323 1320 342 237 177 171 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3535 3716 3879 3535 Size: 181 163 5241 Fragments arranged by size: 5241 181 163 BtsI GCAGTG_nn' Cuts at: 189 733 2520 2828 189 Size: 544 1787 308 2946 Fragments arranged by size: 2946 1787 544 308 DraI TTT'AAA Cuts at: 2584 2584 Size: 5585 DraIII CAC_nnn'GTG Cuts at: 1965 1965 Size: 5585 DrdI GACnn_nn'nnGTC Cuts at: 3291 4149 3291 Size: 858 4727 EaeI y'GGCC_r Cuts at: 464 1826 3170 3344 3735 3762 5107 464 Size: 1362 1344 174 391 27 1345 942 Fragments arranged by size: 1362 1345 1344 942 391 174 27 EagI C'GGCC_G Cuts at: 3170 3170 Size: 5585 EarI CTCTTCn'nnn_ Cuts at: 215 451 2273 3608 3818 215 Size: 236 1822 1335 210 1982 Fragments arranged by size: 1982 1822 1335 236 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3793 4103 4249 5395 3793 Size: 310 146 1146 3983 Fragments arranged by size: 3983 1146 310 146 EcoICRI GAG'CTC Cuts at: 2052 2301 2597 2052 Size: 249 296 5040 Fragments arranged by size: 5040 296 249 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 291 343 574 884 897 943 1575 1621 1732 Size: 52 231 310 13 46 632 46 111 Cuts at: 1732 2133 2317 2368 2455 3410 3842 4589 4857 Size: 401 184 51 87 955 432 747 268 Cuts at: 4857 291 Size: 1019 Fragments arranged by size: 1019 955 747 632 432 401 310 268 231 184 111 87 52 51 46 46 13 EcoNI CCTnn'n_nnAGG Cuts at: 499 2260 499 Size: 1761 3824 EcoO109I rG'GnC_Cy Cuts at: 270 504 1278 1618 2321 2724 270 Size: 234 774 340 703 403 3131 Fragments arranged by size: 3131 774 703 403 340 234 EcoRI G'AATT_C Cuts at: 2589 2589 Size: 5585 EcoRV GAT'ATC Cuts at: 1329 1329 Size: 5585 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3604 3604 Size: 5585 FspI TGC'GCA Cuts at: 3366 3366 Size: 5585 HaeII r_GCGC'y Cuts at: 415 1263 1408 1699 1782 2559 3019 3267 4289 Size: 848 145 291 83 777 460 248 1022 Cuts at: 4289 5268 415 Size: 979 732 Fragments arranged by size: 1022 979 848 777 732 460 291 248 145 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1618 1650 3427 3457 3459 3489 1618 Size: 32 1777 30 2 30 3714 Fragments arranged by size: 3714 1777 32 30 30 2 HincII GTy'rAC Cuts at: 1485 2064 2624 1485 Size: 579 560 4446 Fragments arranged by size: 4446 579 560 KasI G'GCGC_C Cuts at: 3015 3263 3015 Size: 248 5337 KpnI G_GTAC'C Cuts at: 2605 2605 Size: 5585 MluI A'CGCG_T Cuts at: 5018 5018 Size: 5585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 227 4256 4440 227 Size: 4029 184 1372 Fragments arranged by size: 4029 1372 184 MscI TGG'CCA Cuts at: 466 1828 3346 466 Size: 1362 1518 2705 Fragments arranged by size: 2705 1518 1362 MslI CAynn'nnrTG Cuts at: 130 691 2080 2155 3574 3701 130 Size: 561 1389 75 1419 127 2014 Fragments arranged by size: 2014 1419 1389 561 127 75 MspA1I CmG'CkG Cuts at: 1752 1949 2696 3010 3370 4383 4628 1752 Size: 197 747 314 360 1013 245 2709 Fragments arranged by size: 2709 1013 747 360 314 245 197 NaeI GCC'GGC Cuts at: 1194 1611 3766 1194 Size: 417 2155 3013 Fragments arranged by size: 3013 2155 417 NarI GG'CG_CC Cuts at: 3016 3264 3016 Size: 248 5337 NcoI C'CATG_G Cuts at: 125 644 2150 2387 125 Size: 519 1506 237 3323 Fragments arranged by size: 3323 1506 519 237 NgoMIV G'CCGG_C Cuts at: 1192 1609 3764 1192 Size: 417 2155 3013 Fragments arranged by size: 3013 2155 417 NspI r_CATG'y Cuts at: 1159 2479 2638 3138 4045 1159 Size: 1320 159 500 907 2699 Fragments arranged by size: 2699 1320 907 500 159 PciI A'CATG_T Cuts at: 4041 4041 Size: 5585 PflMI CCAn_nnn'nTGG Cuts at: 1965 1965 Size: 5585 PfoI T'CCnGG_A Cuts at: 359 997 2294 2456 359 Size: 638 1297 162 3488 Fragments arranged by size: 3488 1297 638 162 PmeI GTTT'AAAC Cuts at: 2584 2584 Size: 5585 PmlI CAC'GTG Cuts at: 1548 1548 Size: 5585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1522 1554 2215 2247 4724 4756 1522 Size: 32 661 32 2477 32 2351 Fragments arranged by size: 2477 2351 661 32 32 32 PshAI GACnn'nnGTC Cuts at: 646 646 Size: 5585 PspOMI G'GGCC_C Cuts at: 1617 1617 Size: 5585 PspXI vC'TCGA_Gb Cuts at: 2185 2185 Size: 5585 PstI C_TGCA'G Cuts at: 2632 3317 2632 Size: 685 4900 PvuI CG_AT'CG Cuts at: 104 104 Size: 5585 PvuII CAG'CTG Cuts at: 1752 3010 3370 1752 Size: 1258 360 3967 Fragments arranged by size: 3967 1258 360 RsrII CG'GwC_CG Cuts at: 3780 3780 Size: 5585 SacI G_AGCT'C Cuts at: 2054 2303 2599 2054 Size: 249 296 5040 Fragments arranged by size: 5040 296 249 SalI G'TCGA_C Cuts at: 2622 2622 Size: 5585 SapI GCTCTTCn'nnn_ Cuts at: 215 2273 3608 3818 215 Size: 2058 1335 210 1982 Fragments arranged by size: 2058 1982 1335 210 SbfI CC_TGCA'GG Cuts at: 2632 2632 Size: 5585 ScaI AGT'ACT Cuts at: 1630 5102 1630 Size: 3472 2113 SfcI C'TryA_G Cuts at: 33 1203 2211 2628 3313 4306 4497 33 Size: 1170 1008 417 685 993 191 1121 Fragments arranged by size: 1170 1121 1008 993 685 417 191 SfoI GGC'GCC Cuts at: 3017 3265 3017 Size: 248 5337 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5585 SmaI CCC'GGG Cuts at: 2607 2607 Size: 5585 SmlI C'TyrA_G Cuts at: 2185 2257 2740 3655 4147 4409 2185 Size: 72 483 915 492 262 3361 Fragments arranged by size: 3361 915 492 483 262 72 SnaBI TAC'GTA Cuts at: 2577 2577 Size: 5585 SpeI A'CTAG_T Cuts at: 67 67 Size: 5585 SphI G_CATG'C Cuts at: 1159 2479 2638 3138 1159 Size: 1320 159 500 3606 Fragments arranged by size: 3606 1320 500 159 StuI AGG'CCT Cuts at: 1188 1220 1450 2191 1188 Size: 32 230 741 4582 Fragments arranged by size: 4582 741 230 32 StyI C'CwwG_G Cuts at: 125 167 398 407 644 1445 2150 2387 2719 Size: 42 231 9 237 801 705 237 332 Cuts at: 2719 3073 125 Size: 354 2637 Fragments arranged by size: 2637 801 705 354 332 237 237 231 42 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 406 3168 3301 3760 3969 406 Size: 2762 133 459 209 2022 Fragments arranged by size: 2762 2022 459 209 133 TatI w'GTAC_w Cuts at: 1466 1628 1927 5100 1466 Size: 162 299 3173 1951 Fragments arranged by size: 3173 1951 299 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5102 5134 5102 Size: 32 5553 Tth111I GACn'n_nGTC Cuts at: 3382 3382 Size: 5585 XbaI T'CTAG_A Cuts at: 2616 2616 Size: 5585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 693 1878 2280 2394 3080 693 Size: 1185 402 114 686 3198 Fragments arranged by size: 3198 1185 686 402 114 XhoI C'TCGA_G Cuts at: 2185 2185 Size: 5585 XmaI C'CCGG_G Cuts at: 2605 2605 Size: 5585 XmnI GAAnn'nnTTC Cuts at: 1534 1534 Size: 5585 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PpuMI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI ZraI