Restriction map for
pF1KSDA1171
(Circular) (Six-base) MAPSORT of: pF1KSDA1171.seq Check: 4597 from: 1 to: 4814
pF1KSDA1171 4814 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 414 414
Size: 4814
AatII G_ACGT'C
Cuts at: 818 818
Size: 4814
AccI GT'mk_AC
Cuts at: 1852 1852
Size: 4814
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1078 2639 3071 3818 4086 250
Size: 828 1561 432 747 268 978
Fragments arranged by size:
1561 978 828 747 432 268
AfeI AGC'GCT
Cuts at: 766 1204 1447 766
Size: 438 243 4133
Fragments arranged by size:
4133 438 243
AflIII A'CryG_T
Cuts at: 2166 3270 4247 2166
Size: 1104 977 2733
Fragments arranged by size:
2733 1104 977
AgeI A'CCGG_T
Cuts at: 3202 3202
Size: 4814
AhdI GACnn_n'nnGTC
Cuts at: 1185 1185
Size: 4814
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 409 767 235
Size: 174 358 4282
Fragments arranged by size:
4282 358 174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 976 1008 976
Size: 32 4782
AlwNI CAG_nnn'CTG
Cuts at: 189 228 297 389 437 903 1032 1637 2119
Size: 39 69 92 48 466 129 605 482
Cuts at: 2119 3686 189
Size: 1567 1317
Fragments arranged by size:
1567 1317 605 482 466 129 92 69
48 39
ApaI G_GGCC'C
Cuts at: 197 261 197
Size: 64 4750
ApaLI G'TGCA_C
Cuts at: 3584 3584
Size: 4814
ApoI r'AATT_y
Cuts at: 1818 4167 4423 1818
Size: 2349 256 2209
Fragments arranged by size:
2349 2209 256
AscI GG'CGCG_CC
Cuts at: 241 241
Size: 4814
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 403 803 1198 1474 1834 403
Size: 400 395 276 360 3383
Fragments arranged by size:
3383 400 395 360 276
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 426 1830 2244 2492 2527 426
Size: 1404 414 248 35 2713
Fragments arranged by size:
2713 1404 414 248 35
BanII G_rGCy'C
Cuts at: 197 261 1514 1828 2858 197
Size: 64 1253 314 1030 2153
Fragments arranged by size:
2153 1253 1030 314 64
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 810 964 810
Size: 154 4660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1189 1223 1189
Size: 34 4780
BciVI GTATCCnnnnn_n'
Cuts at: 133 2712 3473 4814 133
Size: 2579 761 1341 133
Fragments arranged by size:
2579 1341 761 133
BclI T'GATC_A
Cuts at: 1463 2334 1463
Size: 871 3943
BglI GCCn_nnn'nGGC
Cuts at: 529 538 1771 529
Size: 9 1233 3572
Fragments arranged by size:
3572 1233 9
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 205 1926 205
Size: 1721 3093
Bme1580I G_kGCm'C
Cuts at: 197 261 431 2439 2532 3588 197
Size: 64 170 2008 93 1056 1423
Fragments arranged by size:
2008 1423 1056 170 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 264 1399 1794 2026 2107 2442 4586 264
Size: 1135 395 232 81 335 2144 492
Fragments arranged by size:
2144 1135 492 395 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 925 1003 1380 119
Size: 806 78 377 3553
Fragments arranged by size:
3553 806 377 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1990 2869 3361 3659 853
Size: 1137 879 492 298 2008
Fragments arranged by size:
2008 1137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4518 4518
Size: 4814
BsaAI yAC'GTr
Cuts at: 1806 2167 1806
Size: 361 4453
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 335 815 2245 2493 3195 4691 335
Size: 480 1430 248 702 1496 458
Fragments arranged by size:
1496 1430 702 480 458 248
BsaWI w'CCGG_w
Cuts at: 1405 2003 2524 3202 3476 3623 4034 1405
Size: 598 521 678 274 147 411 2185
Fragments arranged by size:
2185 678 598 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 976 1006 1522 1552 1582 1612 976
Size: 30 516 30 30 30 4178
Fragments arranged by size:
4178 516 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1227 1530 1670 1227
Size: 303 140 4371
Fragments arranged by size:
4371 303 140
BseYI C'CCAG_C
Cuts at: 430 2239 2997 3574 4070 430
Size: 1809 758 577 496 1174
Fragments arranged by size:
1809 1174 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 535 1462 195
Size: 340 927 3547
Fragments arranged by size:
3547 927 340
BsiEI CG_ry'CG
Cuts at: 104 533 2356 2402 3610 104
Size: 429 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 429 46
BsiHKAI G_wGCw'C
Cuts at: 1828 2606 2796 3588 1828
Size: 778 190 792 3054
Fragments arranged by size:
3054 792 778 190
BsmI GAATG_Cn'
Cuts at: 450 1417 450
Size: 967 3847
Bsp1286I G_dGCh'C
Cuts at: 197 261 431 1514 1828 2439 2532 2606 2796
Size: 64 170 1083 314 611 93 74 190
Cuts at: 2796 2858 3588 197
Size: 62 730 1423
Fragments arranged by size:
1423 1083 730 611 314 190 170 93
74 64 62
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 3990 3990
Size: 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 414 735 1601 1864 2078 2380 2761 414
Size: 321 866 263 214 302 381 2467
Fragments arranged by size:
2467 866 381 321 302 263 214
BsrBI CCG'CTC
Cuts at: 50 1148 1579 3106 3160 4644 4809 50
Size: 1098 431 1527 54 1484 165 55
Fragments arranged by size:
1527 1484 1098 431 165 55 54
BsrDI GCAATG_nn'
Cuts at: 615 1757 2726 615
Size: 1142 969 2703
Fragments arranged by size:
2703 1142 969
BsrFI r'CCGG_y
Cuts at: 528 2812 2993 3202 528
Size: 2284 181 209 2140
Fragments arranged by size:
2284 2140 209 181
BsrGI T'GTAC_A
Cuts at: 344 1116 344
Size: 772 4042
BssHII G'CGCG_C
Cuts at: 241 241
Size: 4814
BssSI C'ACGA_G
Cuts at: 3085 3443 3085
Size: 358 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 389 1637 2191 389
Size: 1248 554 3012
Fragments arranged by size:
3012 1248 554
BstBI TT'CG_AA
Cuts at: 110 3175 110
Size: 3065 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1620 1620
Size: 4814
BstYI r'GATC_y
Cuts at: 1 473 1022 1839 2306 2329 2664 2910 3922
Size: 472 549 817 467 23 335 246 1012
Cuts at: 3922 4008 4020 4366 1
Size: 86 12 346 449
Fragments arranged by size:
1012 817 549 472 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1436 1436
Size: 4814
BtgI C'CryG_G
Cuts at: 125 533 1766 125
Size: 408 1233 3173
Fragments arranged by size:
3173 1233 408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 936 2764 2945 3108 936
Size: 1828 181 163 2642
Fragments arranged by size:
2642 1828 181 163
BtsI GCAGTG_nn'
Cuts at: 1710 2057 1710
Size: 347 4467
ClaI AT'CG_AT
Cuts at: 1679 1679
Size: 4814
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 488 530 722 860 1040 1124 1262 1574 1662
Size: 42 192 138 180 84 138 312 88
Cuts at: 1662 2399 2573 2964 2991 4336 488
Size: 737 174 391 27 1345 966
Fragments arranged by size:
1345 966 737 391 312 192 180 174
138 138 88 84 42 27
EagI C'GGCC_G
Cuts at: 530 2399 530
Size: 1869 2945
EarI CTCTTCn'nnn_
Cuts at: 1081 2837 3047 1081
Size: 1756 210 2848
Fragments arranged by size:
2848 1756 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 991 1524 3022 3332 3478 4624 991
Size: 533 1498 310 146 1146 1181
Fragments arranged by size:
1498 1181 1146 533 310 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 250 925 1003 1078 1380 2639 3071 3818
Size: 131 675 78 75 302 1259 432 747
Cuts at: 3818 4086 119
Size: 268 847
Fragments arranged by size:
1259 847 747 675 432 302 268 131
78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 826 826
Size: 4814
EcoO109I rG'GnC_Cy
Cuts at: 186 193 678 1194 1686 1788 1953 186
Size: 7 485 516 492 102 165 3047
Fragments arranged by size:
3047 516 492 485 165 102 7
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 469 2595 469
Size: 2126 2688
HaeII r_GCGC'y
Cuts at: 297 768 859 1206 1449 1672 2248 2496 3518
Size: 471 91 347 243 223 576 248 1022
Cuts at: 3518 4497 297
Size: 979 614
Fragments arranged by size:
1022 979 614 576 471 347 248 243
223 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1171 1203 1546 1578 2656 2686 2688 2718 1171
Size: 32 343 32 1078 30 2 30 3267
Fragments arranged by size:
3267 1078 343 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1853 1853
Size: 4814
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 890 3485 3669 710
Size: 180 2595 184 1855
Fragments arranged by size:
2595 1855 184 180
MscI TGG'CCA
Cuts at: 490 862 1042 1264 2575 490
Size: 372 180 222 1311 2729
Fragments arranged by size:
2729 1311 372 222 180
MslI CAynn'nnrTG
Cuts at: 424 1075 1618 2803 2930 424
Size: 651 543 1185 127 2308
Fragments arranged by size:
2308 1185 651 543 127
MspA1I CmG'CkG
Cuts at: 237 389 399 903 1552 1925 2239 2599 3612
Size: 152 10 504 649 373 314 360 1013
Cuts at: 3612 3857 237
Size: 245 1194
Fragments arranged by size:
1194 1013 649 504 373 360 314 245
152 10
NaeI GCC'GGC
Cuts at: 530 2995 530
Size: 2465 2349
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NcoI C'CATG_G
Cuts at: 125 125
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 528 2993 528
Size: 2465 2349
NruI TCG'CGA
Cuts at: 955 955
Size: 4814
NspI r_CATG'y
Cuts at: 1867 2367 3274 1867
Size: 500 907 3407
Fragments arranged by size:
3407 907 500
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 189 1358 189
Size: 1169 3645
PfoI T'CCnGG_A
Cuts at: 135 182 302 1784 135
Size: 47 120 1482 3165
Fragments arranged by size:
3165 1482 120 47
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 1132 1164 1600 1632 3953 3985 976
Size: 32 124 32 436 32 2321 32 1805
Fragments arranged by size:
2321 1805 436 124 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 186 678 1194 1686 1788 186
Size: 492 516 492 102 3212
Fragments arranged by size:
3212 516 492 492 102
PspOMI G'GGCC_C
Cuts at: 193 257 193
Size: 64 4750
PstI C_TGCA'G
Cuts at: 224 746 1037 1247 1433 1861 2546 224
Size: 522 291 210 186 428 685 2492
Fragments arranged by size:
2492 685 522 428 291 210 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 237 389 2239 2599 237
Size: 152 1850 360 2452
Fragments arranged by size:
2452 1850 360 152
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SanDI GG'GwC_CC
Cuts at: 1194 1194
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
SbfI CC_TGCA'GG
Cuts at: 746 1861 746
Size: 1115 3699
ScaI AGT'ACT
Cuts at: 694 4331 694
Size: 3637 1177
SexAI A'CCwGG_T
Cuts at: 680 680
Size: 4814
SfcI C'TryA_G
Cuts at: 33 220 555 742 1033 1243 1429 1857 2542
Size: 187 335 187 291 210 186 428 685
Cuts at: 2542 3535 3726 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 428 335 291 210 191
187 187 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 538 538
Size: 4814
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 805 1836 805
Size: 1031 3783
SmlI C'TyrA_G
Cuts at: 868 1969 2884 3376 3638 868
Size: 1101 915 492 262 2044
Fragments arranged by size:
2044 1101 915 492 262
SnaBI TAC'GTA
Cuts at: 1806 1806
Size: 4814
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StuI AGG'CCT
Cuts at: 589 1774 589
Size: 1185 3629
StyI C'CwwG_G
Cuts at: 125 197 1948 2302 125
Size: 72 1751 354 2637
Fragments arranged by size:
2637 1751 354 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1621 1813 2397 2530 2989 3198 1446
Size: 175 192 584 133 459 209 3062
Fragments arranged by size:
3062 584 459 209 192 175 133
TatI w'GTAC_w
Cuts at: 344 692 1116 4329 344
Size: 348 424 3213 829
Fragments arranged by size:
3213 829 424 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1522 1554 4331 4363 1522
Size: 32 2777 32 1973
Fragments arranged by size:
2777 1973 32 32
Tth111I GACn'n_nGTC
Cuts at: 963 1608 2611 963
Size: 645 1003 3166
Fragments arranged by size:
3166 1003 645
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 2309 186
Size: 2123 2691
XmaI C'CCGG_G
Cuts at: 803 1834 803
Size: 1031 3783
XmnI GAAnn'nnTTC
Cuts at: 991 991
Size: 4814
ZraI GAC'GTC
Cuts at: 816 816
Size: 4814
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BstEII BstZ17I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SgrAI SrfI SspI SwaI XhoI