Restriction map for
pF1KSDA1186
(Circular) (Six-base) MAPSORT of: pF1KSDA1186.seq Check: 1354 from: 1 to: 4346
pF1KSDA1186 4346 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1195 1195
Size: 4346
AccI GT'mk_AC
Cuts at: 1384 1384
Size: 4346
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1105 1190 2171 2603 3350 3618 703
Size: 402 85 981 432 747 268 1431
Fragments arranged by size:
1431 981 747 432 402 268 85
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 375 483 1651 3218 375
Size: 108 1168 1567 1503
Fragments arranged by size:
1567 1503 1168 108
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 1366 1366
Size: 4346
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 834 853 1324 1362 1776 2024 2059 834
Size: 19 471 38 414 248 35 3121
Fragments arranged by size:
3121 471 414 248 38 35 19
BanII G_rGCy'C
Cuts at: 154 1360 2390 154
Size: 1206 1030 2110
Fragments arranged by size:
2110 1206 1030
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BbsI GAAGACnn'nnnn_
Cuts at: 1093 1093
Size: 4346
BbvCI CC'TCA_GC
Cuts at: 487 487
Size: 4346
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3005 4346 2244
Size: 761 1341 2244
Fragments arranged by size:
2244 1341 761
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 534 856 1971 2064 3120 534
Size: 322 1115 93 1056 1760
Fragments arranged by size:
1760 1115 1056 322 93
BmrI ACTGGGnnnn_n'
Cuts at: 409 1558 1639 1974 4118 409
Size: 1149 81 335 2144 637
Fragments arranged by size:
2144 1149 637 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 664 2713 664
Size: 2049 2297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 338
Size: 4346
Bpu10I CC'TnA_GC
Cuts at: 487 487
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1522 2401 2893 3191 589
Size: 933 879 492 298 1744
Fragments arranged by size:
1744 933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 932 4050 559
Size: 373 3118 855
Fragments arranged by size:
3118 855 373
BsaAI yAC'GTr
Cuts at: 1338 1699 1338
Size: 361 3985
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 1777 2025 2727 4223 1777
Size: 248 702 1496 1900
Fragments arranged by size:
1900 1496 702 248
BsaWI w'CCGG_w
Cuts at: 293 1535 2056 2734 3008 3155 3566 293
Size: 1242 521 678 274 147 411 1073
Fragments arranged by size:
1242 1073 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1049 1079 1049
Size: 30 4316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 978 978
Size: 4346
BseYI C'CCAG_C
Cuts at: 1314 1771 2529 3106 3602 1314
Size: 457 758 577 496 2058
Fragments arranged by size:
2058 758 577 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 991
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1360 2138 2328 3120 1360
Size: 778 190 792 2586
Fragments arranged by size:
2586 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 154 534 856 1360 1971 2064 2138 2328 2390
Size: 380 322 504 611 93 74 190 62
Cuts at: 2390 3120 154
Size: 730 1380
Fragments arranged by size:
1380 730 611 504 380 322 190 93
74 62
BspEI T'CCGG_A
Cuts at: 293 3566 293
Size: 3273 1073
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 491 1195 1396 1610 1912 2293 278
Size: 213 704 201 214 302 381 2331
Fragments arranged by size:
2331 704 381 302 214 213 201
BsrBI CCG'CTC
Cuts at: 50 131 1060 2638 2692 4176 4341 50
Size: 81 929 1578 54 1484 165 55
Fragments arranged by size:
1578 1484 929 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 207 2344 2525 2734 207
Size: 2137 181 209 1819
Fragments arranged by size:
2137 1819 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 110 2707 110
Size: 2597 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 549 549
Size: 4346
BstYI r'GATC_y
Cuts at: 1 1010 1371 1838 1861 2196 2442 3454 3540
Size: 1009 361 467 23 335 246 1012 86
Cuts at: 3540 3552 3898 1
Size: 12 346 449
Fragments arranged by size:
1012 1009 467 449 361 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1033 1033
Size: 4346
BtgI C'CryG_G
Cuts at: 981 981
Size: 4346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 1197 1589 1197
Size: 392 3954
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DraIII CAC_nnn'GTG
Cuts at: 969 999 969
Size: 30 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 173 1931 2105 2496 2523 3868 173
Size: 1758 174 391 27 1345 651
Fragments arranged by size:
1758 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 1021 1292 2369 2579 1021
Size: 271 1077 210 2788
Fragments arranged by size:
2788 1077 271 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2554 2864 3010 4156 2554
Size: 310 146 1146 2744
Fragments arranged by size:
2744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 703 1105 1190 2171 2603 3350 3618 338
Size: 365 402 85 981 432 747 268 1066
Fragments arranged by size:
1066 981 747 432 402 365 268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 4346
EcoO109I rG'GnC_Cy
Cuts at: 480 1178 1272 1485 480
Size: 698 94 213 3341
Fragments arranged by size:
3341 698 213 94
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 1780 2028 3050 4029 1780
Size: 248 1022 979 2097
Fragments arranged by size:
2097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 2188 2218 2220 2250 1276
Size: 32 880 30 2 30 3372
Fragments arranged by size:
3372 880 32 30 30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
HindIII A'AGCT_T
Cuts at: 261 261
Size: 4346
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 260 390 3017 3201 260
Size: 130 2627 184 1405
Fragments arranged by size:
2627 1405 184 130
MscI TGG'CCA
Cuts at: 175 2107 175
Size: 1932 2414
MslI CAynn'nnrTG
Cuts at: 2335 2462 2335
Size: 127 4219
MspA1I CmG'CkG
Cuts at: 1110 1314 1457 1771 2131 3144 3389 1110
Size: 204 143 314 360 1013 245 2067
Fragments arranged by size:
2067 1013 360 314 245 204 143
NaeI GCC'GGC
Cuts at: 209 2527 209
Size: 2318 2028
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NgoMIV G'CCGG_C
Cuts at: 207 2525 207
Size: 2318 2028
NspI r_CATG'y
Cuts at: 304 657 1399 1899 2806 304
Size: 353 742 500 907 1844
Fragments arranged by size:
1844 907 742 500 353
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 999 999
Size: 4346
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 480 1178 1272 480
Size: 698 94 3554
Fragments arranged by size:
3554 698 94
PstI C_TGCA'G
Cuts at: 275 1192 1393 2078 275
Size: 917 201 685 2543
Fragments arranged by size:
2543 917 685 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 1314 1771 2131 1314
Size: 457 360 3529
Fragments arranged by size:
3529 457 360
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SanDI GG'GwC_CC
Cuts at: 1178 1178
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SexAI A'CCwGG_T
Cuts at: 830 941 1160 830
Size: 111 219 4016
Fragments arranged by size:
4016 219 111
SfcI C'TryA_G
Cuts at: 33 271 1188 1230 1389 2074 3067 3258 33
Size: 238 917 42 159 685 993 191 1121
Fragments arranged by size:
1121 993 917 685 238 191 159 42
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 604 1501 2416 2908 3170 604
Size: 897 915 492 262 1780
Fragments arranged by size:
1780 915 897 492 262
SnaBI TAC'GTA
Cuts at: 1338 1338
Size: 4346
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StuI AGG'CCT
Cuts at: 634 634
Size: 4346
StyI C'CwwG_G
Cuts at: 449 1480 1834 449
Size: 1031 354 2961
Fragments arranged by size:
2961 1031 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1929 2062 2521 2730 1929
Size: 133 459 209 3545
Fragments arranged by size:
3545 459 209 133
TatI w'GTAC_w
Cuts at: 3861 3861
Size: 4346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4314
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI