Restriction map for
pF1KSDA1187
(Circular) (Six-base) MAPSORT of: pF1KSDA1187.seq Check: 6824 from: 1 to: 5548
pF1KSDA1187 5548 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2584 2584
Size: 5548
Acc65I G'GTAC_C
Cuts at: 2562 2562
Size: 5548
AclI AA'CG_TT
Cuts at: 5388 5388
Size: 5548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 3371 3803 4552 4820 413
Size: 2958 432 749 268 1141
Fragments arranged by size:
2958 1141 749 432 268
AfeI AGC'GCT
Cuts at: 754 814 754
Size: 60 5488
AflIII A'CryG_T
Cuts at: 2898 4004 4981 2898
Size: 1106 977 3465
Fragments arranged by size:
3465 1106 977
AgeI A'CCGG_T
Cuts at: 3936 3936
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 719 1460 2365 719
Size: 741 905 3902
Fragments arranged by size:
3902 905 741
AlwNI CAG_nnn'CTG
Cuts at: 1341 1726 1753 2088 2245 2851 4420 1341
Size: 385 27 335 157 606 1569 2469
Fragments arranged by size:
2469 1569 606 385 335 157 27
ApaI G_GGCC'C
Cuts at: 433 834 1583 433
Size: 401 749 4398
Fragments arranged by size:
4398 749 401
ApaLI G'TGCA_C
Cuts at: 1150 4318 1150
Size: 3168 2380
ApoI r'AATT_y
Cuts at: 2550 4901 5157 2550
Size: 2351 256 2941
Fragments arranged by size:
2941 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5548
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5548
AvaI C'yCGr_G
Cuts at: 736 1310 1505 1582 1844 1940 2566 736
Size: 574 195 77 262 96 626 3718
Fragments arranged by size:
3718 626 574 262 195 96 77
AvrII C'CTAG_G
Cuts at: 355 355
Size: 5548
BamHI G'GATC_C
Cuts at: 2571 2571
Size: 5548
BanI G'GyrC_C
Cuts at: 662 1510 1650 1662 1789 1835 2562 2976 3224
Size: 848 140 12 127 46 727 414 248
Cuts at: 3224 3259 662
Size: 35 2951
Fragments arranged by size:
2951 848 727 414 248 140 127 46
35 12
BanII G_rGCy'C
Cuts at: 337 433 834 1391 1567 1583 2309 2560 3590
Size: 96 401 557 176 16 726 251 1030
Cuts at: 3590 337
Size: 2295
Fragments arranged by size:
2295 1030 726 557 401 251 176 96
16
BbeI G_GCGC'C
Cuts at: 666 1666 1839 2980 3228 666
Size: 1000 173 1141 248 2986
Fragments arranged by size:
2986 1141 1000 248 173
BbsI GAAGACnn'nnnn_
Cuts at: 793 793
Size: 5548
BciVI GTATCCnnnnn_n'
Cuts at: 3444 4207 5548 3444
Size: 763 1341 3444
Fragments arranged by size:
3444 1341 763
BclI T'GATC_A
Cuts at: 3066 3066
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 595 1042 1648 595
Size: 447 606 4495
Fragments arranged by size:
4495 606 447
BglII A'GATC_T
Cuts at: 3061 5100 3061
Size: 2039 3509
BlpI GC'TnA_GC
Cuts at: 880 1390 2658 880
Size: 510 1268 3770
Fragments arranged by size:
3770 1268 510
Bme1580I G_kGCm'C
Cuts at: 433 834 1099 1154 1305 1362 1583 3171 3264
Size: 401 265 55 151 57 221 1588 93
Cuts at: 3264 4322 433
Size: 1058 1659
Fragments arranged by size:
1659 1588 1058 401 265 221 151 93
57 55
BmgBI CAC'GTC
Cuts at: 791 791
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 2758 2839 3174 5320 2758
Size: 81 335 2146 2986
Fragments arranged by size:
2986 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 354 3913 354
Size: 3559 1989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 628 634 691 733 833 2110 2170 194
Size: 434 6 57 42 100 1277 60 3572
Fragments arranged by size:
3572 1277 434 100 60 57 42 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 2476 2722 3601 4095 4393 1309
Size: 1167 246 879 494 298 2464
Fragments arranged by size:
2464 1167 879 494 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 1705 5252 1705
Size: 3547 2001
BsaAI yAC'GTr
Cuts at: 1019 1357 2538 2899 1019
Size: 338 1181 361 3668
Fragments arranged by size:
3668 1181 361 338
BsaBI GATnn'nnATC
Cuts at: 6 3060 5099 6
Size: 3054 2039 455
Fragments arranged by size:
3054 2039 455
BsaHI Gr'CG_yC
Cuts at: 663 919 1126 1663 1836 2977 3225 3929 5425
Size: 256 207 537 173 1141 248 704 1496
Cuts at: 5425 663
Size: 786
Fragments arranged by size:
1496 1141 786 704 537 256 248 207
173
BsaWI w'CCGG_w
Cuts at: 1218 2735 3256 3936 4210 4357 4768 1218
Size: 1517 521 680 274 147 411 1998
Fragments arranged by size:
1998 1517 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1470 1500 2251 2281 2311 2341 1470
Size: 30 751 30 30 30 4677
Fragments arranged by size:
4677 751 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 440 631 688 1564 1879 1927 1990 2029 2059
Size: 191 57 876 315 48 63 39 30
Cuts at: 2059 2167 2317 440
Size: 108 150 3671
Fragments arranged by size:
3671 876 315 191 150 108 63 57
48 39 30
BseYI C'CCAG_C
Cuts at: 150 168 1459 1567 1638 1677 1746 2385 2971
Size: 18 1291 108 71 39 69 639 586
Cuts at: 2971 3729 4308 4804 150
Size: 758 579 496 894
Fragments arranged by size:
1291 894 758 639 586 579 496 108
71 69 39 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 820 1152 1270 2314 2527 725
Size: 95 332 118 1044 213 3746
Fragments arranged by size:
3746 1044 332 213 118 95
BsiEI CG_ry'CG
Cuts at: 104 978 1065 1234 2062 3088 3134 4344 104
Size: 874 87 169 828 1026 46 1210 1308
Fragments arranged by size:
1308 1210 1026 874 828 169 87 46
BsiHKAI G_wGCw'C
Cuts at: 1154 1391 2560 3338 3528 4322 1154
Size: 237 1169 778 190 794 2380
Fragments arranged by size:
2380 1169 794 778 237 190
BsmI GAATG_Cn'
Cuts at: 300 1910 300
Size: 1610 3938
Bsp1286I G_dGCh'C
Cuts at: 337 433 834 1099 1154 1305 1362 1391 1567
Size: 96 401 265 55 151 57 29 176
Cuts at: 1567 1583 2309 2560 3171 3264 3338 3528 3590
Size: 16 726 251 611 93 74 190 62
Cuts at: 3590 4322 337
Size: 732 1563
Fragments arranged by size:
1563 732 726 611 401 265 251 190
176 151 96 93 74 62 57 55
29 16
BspEI T'CCGG_A
Cuts at: 4768 4768
Size: 5548
BspHI T'CATG_A
Cuts at: 2159 4724 2159
Size: 2565 2983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 2508 2596 2810 3112 3493 336
Size: 2172 88 214 302 381 2391
Fragments arranged by size:
2391 2172 381 302 214 88
BsrBI CCG'CTC
Cuts at: 50 134 558 570 1216 1278 1875 1950 2025
Size: 84 424 12 646 62 597 75 75
Cuts at: 2025 2079 2094 2127 3838 3892 5378 5543 50
Size: 54 15 33 1711 54 1486 165 55
Fragments arranged by size:
1711 1486 646 597 424 165 84 75
75 62 55 54 54 33 15 12
BsrDI GCAATG_nn'
Cuts at: 3458 3458
Size: 5548
BsrFI r'CCGG_y
Cuts at: 659 665 1089 1168 1229 1245 1647 1659 1786
Size: 6 424 79 61 16 402 12 127
Cuts at: 1786 3544 3725 3936 659
Size: 1758 181 211 2271
Fragments arranged by size:
2271 1758 424 402 211 181 127 79
61 16 12 6
BssHII G'CGCG_C
Cuts at: 1045 1045
Size: 5548
BssSI C'ACGA_G
Cuts at: 1988 3817 4177 1988
Size: 1829 360 3359
Fragments arranged by size:
3359 1829 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2923 2923
Size: 5548
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5548
BstEII G'GTnAC_C
Cuts at: 217 1764 217
Size: 1547 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 582 2401 582
Size: 1819 3729
BstYI r'GATC_y
Cuts at: 1 2571 3038 3061 3396 3642 4656 4742 4754
Size: 2570 467 23 335 246 1014 86 12
Cuts at: 4754 5100 1
Size: 346 449
Fragments arranged by size:
2570 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 339 339
Size: 5548
BtgI C'CryG_G
Cuts at: 125 425 433 1706 125
Size: 300 8 1273 3967
Fragments arranged by size:
3967 1273 300 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3496 3677 3840 3496
Size: 181 163 5204
Fragments arranged by size:
5204 181 163
BtsI GCAGTG_nn'
Cuts at: 488 605 961 1514 2789 488
Size: 117 356 553 1275 3247
Fragments arranged by size:
3247 1275 553 356 117
DraI TTT'AAA
Cuts at: 2545 2545
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 1729 2175 3252 4112 1729
Size: 446 1077 860 3165
Fragments arranged by size:
3165 1077 860 446
EaeI y'GGCC_r
Cuts at: 587 975 1231 1784 3131 3305 3696 3723 5070
Size: 388 256 553 1347 174 391 27 1347
Cuts at: 5070 587
Size: 1065
Fragments arranged by size:
1347 1347 1065 553 391 388 256 174
27
EagI C'GGCC_G
Cuts at: 975 1231 3131 975
Size: 256 1900 3392
Fragments arranged by size:
3392 1900 256
EarI CTCTTCn'nnn_
Cuts at: 371 560 1291 2063 2158 3569 3779 371
Size: 189 731 772 95 1411 210 2140
Fragments arranged by size:
2140 1411 772 731 210 189 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1101 1359 1960 1972 2089 3754 4066 4212 5358
Size: 258 601 12 117 1665 312 146 1146
Cuts at: 5358 1101
Size: 1291
Fragments arranged by size:
1665 1291 1146 601 312 258 146 117
12
EcoICRI GAG'CTC
Cuts at: 1389 2558 1389
Size: 1169 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 413 628 634 691 733 833 2110 2170
Size: 219 215 6 57 42 100 1277 60
Cuts at: 2170 3371 3803 4552 4820 194
Size: 1201 432 749 268 922
Fragments arranged by size:
1277 1201 922 749 432 268 219 215
100 60 57 42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1007 1437 1007
Size: 430 5118
EcoO109I rG'GnC_Cy
Cuts at: 185 409 830 831 1012 1579 2685 185
Size: 224 421 1 181 567 1106 3048
Fragments arranged by size:
3048 1106 567 421 224 181 1
EcoRI G'AATT_C
Cuts at: 2550 2550
Size: 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3565 3565
Size: 5548
FspI TGC'GCA
Cuts at: 3327 3327
Size: 5548
HaeII r_GCGC'y
Cuts at: 634 660 666 756 816 1188 1335 1574 1666
Size: 26 6 90 60 372 147 239 92
Cuts at: 1666 1839 2980 3228 4252 5231 634
Size: 173 1141 248 1024 979 951
Fragments arranged by size:
1141 1024 979 951 372 248 239 173
147 92 90 60 26 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 909 941 1612 1644 2251 2283 3388 3418 3420
Size: 32 671 32 607 32 1105 30 2
Cuts at: 3420 3450 909
Size: 30 3007
Fragments arranged by size:
3007 1105 671 607 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2585 2585
Size: 5548
KasI G'GCGC_C
Cuts at: 662 1662 1835 2976 3224 662
Size: 1000 173 1141 248 2986
Fragments arranged by size:
2986 1141 1000 248 173
KpnI G_GTAC'C
Cuts at: 2566 2566
Size: 5548
MluI A'CGCG_T
Cuts at: 4981 4981
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 499 518 795 4219 4403 499
Size: 19 277 3424 184 1644
Fragments arranged by size:
3424 1644 277 184 19
MscI TGG'CCA
Cuts at: 3307 3307
Size: 5548
MslI CAynn'nnrTG
Cuts at: 3535 3662 3535
Size: 127 5421
MspA1I CmG'CkG
Cuts at: 172 726 879 1157 1708 1854 1920 2657 2971
Size: 554 153 278 551 146 66 737 314
Cuts at: 2971 3331 4346 4591 172
Size: 360 1015 245 1129
Fragments arranged by size:
1129 1015 737 554 551 360 314 278
245 153 146 66
NaeI GCC'GGC
Cuts at: 661 667 1231 1661 1788 3727 661
Size: 6 564 430 127 1939 2482
Fragments arranged by size:
2482 1939 564 430 127 6
NarI GG'CG_CC
Cuts at: 663 1663 1836 2977 3225 663
Size: 1000 173 1141 248 2986
Fragments arranged by size:
2986 1141 1000 248 173
NcoI C'CATG_G
Cuts at: 125 425 125
Size: 300 5248
NgoMIV G'CCGG_C
Cuts at: 659 665 1229 1659 1786 3725 659
Size: 6 564 430 127 1939 2482
Fragments arranged by size:
2482 1939 564 430 127 6
NruI TCG'CGA
Cuts at: 3909 3909
Size: 5548
NspI r_CATG'y
Cuts at: 2599 3099 4008 2599
Size: 500 909 4139
Fragments arranged by size:
4139 909 500
PasI CC'CwG_GG
Cuts at: 1003 1465 2272 1003
Size: 462 807 4279
Fragments arranged by size:
4279 807 462
PciI A'CATG_T
Cuts at: 4004 4004
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1433 1433
Size: 5548
PfoI T'CCnGG_A
Cuts at: 650 849 650
Size: 199 5349
PmeI GTTT'AAAC
Cuts at: 2545 2545
Size: 5548
PmlI CAC'GTG
Cuts at: 1019 1357 1019
Size: 338 5210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4687 4719 4687
Size: 32 5516
PpuMI rG'GwC_Cy
Cuts at: 185 409 185
Size: 224 5324
PshAI GACnn'nnGTC
Cuts at: 2435 2435
Size: 5548
PspOMI G'GGCC_C
Cuts at: 429 830 1579 429
Size: 401 749 4398
Fragments arranged by size:
4398 749 401
PstI C_TGCA'G
Cuts at: 878 1083 1886 2033 2282 2378 2593 3278 878
Size: 205 803 147 249 96 215 685 3148
Fragments arranged by size:
3148 803 685 249 215 205 147 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5548
PvuII CAG'CTG
Cuts at: 172 879 1920 2971 3331 172
Size: 707 1041 1051 360 2389
Fragments arranged by size:
2389 1051 1041 707 360
RsrII CG'GwC_CG
Cuts at: 3741 3741
Size: 5548
SacI G_AGCT'C
Cuts at: 1391 2560 1391
Size: 1169 4379
SacII CC_GC'GG
Cuts at: 1709 1709
Size: 5548
SalI G'TCGA_C
Cuts at: 2583 2583
Size: 5548
SapI GCTCTTCn'nnn_
Cuts at: 560 3569 3779 560
Size: 3009 210 2329
Fragments arranged by size:
3009 2329 210
SbfI CC_TGCA'GG
Cuts at: 2593 2593
Size: 5548
ScaI AGT'ACT
Cuts at: 5065 5065
Size: 5548
SfcI C'TryA_G
Cuts at: 33 874 1079 1718 1882 2029 2278 2374 2589
Size: 841 205 639 164 147 249 96 215
Cuts at: 2589 3274 4269 4460 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 841 685 639 249 215 205
191 164 147 96
SfoI GGC'GCC
Cuts at: 664 1664 1837 2978 3226 664
Size: 1000 173 1141 248 2986
Fragments arranged by size:
2986 1141 1000 248 173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 659 1168 1245 1659 659
Size: 509 77 414 4548
Fragments arranged by size:
4548 509 414 77
SmaI CCC'GGG
Cuts at: 1312 1846 1942 2568 1312
Size: 534 96 626 4292
Fragments arranged by size:
4292 626 534 96
SmlI C'TyrA_G
Cuts at: 736 1324 2491 2701 3616 4110 4372 736
Size: 588 1167 210 915 494 262 1912
Fragments arranged by size:
1912 1167 915 588 494 262 210
SnaBI TAC'GTA
Cuts at: 2538 2538
Size: 5548
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5548
SphI G_CATG'C
Cuts at: 2599 3099 2599
Size: 500 5048
SrfI GCCC'GGGC
Cuts at: 1942 1942
Size: 5548
StuI AGG'CCT
Cuts at: 1240 1443 1240
Size: 203 5345
StyI C'CwwG_G
Cuts at: 125 355 425 626 1403 1526 2680 3034 125
Size: 230 70 201 777 123 1154 354 2639
Fragments arranged by size:
2639 1154 777 354 230 201 123 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1814 2048 2255 3129 3262 3721 3932 1814
Size: 234 207 874 133 459 211 3430
Fragments arranged by size:
3430 874 459 234 211 207 133
TatI w'GTAC_w
Cuts at: 5063 5063
Size: 5548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1470 1502 2311 2343 5065 5097 1470
Size: 32 809 32 2722 32 1921
Fragments arranged by size:
2722 1921 809 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3343 3343
Size: 5548
XbaI T'CTAG_A
Cuts at: 2577 2577
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1818 3041 1818
Size: 1223 4325
XhoI C'TCGA_G
Cuts at: 736 736
Size: 5548
XmaI C'CCGG_G
Cuts at: 1310 1844 1940 2566 1310
Size: 534 96 626 4292
Fragments arranged by size:
4292 626 534 96
XmnI GAAnn'nnTTC
Cuts at: 2404 2404
Size: 5548
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PsiI
PspXI PsrI SanDI SexAI SfiI SspI SwaI ZraI