(Circular) (Six-base) MAPSORT of: pF1KSDA1187.seq Check: 6824 from: 1 to: 5548 pF1KSDA1187 5548 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AccI GT'mk_AC Cuts at: 2584 2584 Size: 5548 Acc65I G'GTAC_C Cuts at: 2562 2562 Size: 5548 AclI AA'CG_TT Cuts at: 5388 5388 Size: 5548 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 413 3371 3803 4552 4820 413 Size: 2958 432 749 268 1141 Fragments arranged by size: 2958 1141 749 432 268 AfeI AGC'GCT Cuts at: 754 814 754 Size: 60 5488 AflIII A'CryG_T Cuts at: 2898 4004 4981 2898 Size: 1106 977 3465 Fragments arranged by size: 3465 1106 977 AgeI A'CCGG_T Cuts at: 3936 3936 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 719 1460 2365 719 Size: 741 905 3902 Fragments arranged by size: 3902 905 741 AlwNI CAG_nnn'CTG Cuts at: 1341 1726 1753 2088 2245 2851 4420 1341 Size: 385 27 335 157 606 1569 2469 Fragments arranged by size: 2469 1569 606 385 335 157 27 ApaI G_GGCC'C Cuts at: 433 834 1583 433 Size: 401 749 4398 Fragments arranged by size: 4398 749 401 ApaLI G'TGCA_C Cuts at: 1150 4318 1150 Size: 3168 2380 ApoI r'AATT_y Cuts at: 2550 4901 5157 2550 Size: 2351 256 2941 Fragments arranged by size: 2941 2351 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5548 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5548 AvaI C'yCGr_G Cuts at: 736 1310 1505 1582 1844 1940 2566 736 Size: 574 195 77 262 96 626 3718 Fragments arranged by size: 3718 626 574 262 195 96 77 AvrII C'CTAG_G Cuts at: 355 355 Size: 5548 BamHI G'GATC_C Cuts at: 2571 2571 Size: 5548 BanI G'GyrC_C Cuts at: 662 1510 1650 1662 1789 1835 2562 2976 3224 Size: 848 140 12 127 46 727 414 248 Cuts at: 3224 3259 662 Size: 35 2951 Fragments arranged by size: 2951 848 727 414 248 140 127 46 35 12 BanII G_rGCy'C Cuts at: 337 433 834 1391 1567 1583 2309 2560 3590 Size: 96 401 557 176 16 726 251 1030 Cuts at: 3590 337 Size: 2295 Fragments arranged by size: 2295 1030 726 557 401 251 176 96 16 BbeI G_GCGC'C Cuts at: 666 1666 1839 2980 3228 666 Size: 1000 173 1141 248 2986 Fragments arranged by size: 2986 1141 1000 248 173 BbsI GAAGACnn'nnnn_ Cuts at: 793 793 Size: 5548 BciVI GTATCCnnnnn_n' Cuts at: 3444 4207 5548 3444 Size: 763 1341 3444 Fragments arranged by size: 3444 1341 763 BclI T'GATC_A Cuts at: 3066 3066 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 595 1042 1648 595 Size: 447 606 4495 Fragments arranged by size: 4495 606 447 BglII A'GATC_T Cuts at: 3061 5100 3061 Size: 2039 3509 BlpI GC'TnA_GC Cuts at: 880 1390 2658 880 Size: 510 1268 3770 Fragments arranged by size: 3770 1268 510 Bme1580I G_kGCm'C Cuts at: 433 834 1099 1154 1305 1362 1583 3171 3264 Size: 401 265 55 151 57 221 1588 93 Cuts at: 3264 4322 433 Size: 1058 1659 Fragments arranged by size: 1659 1588 1058 401 265 221 151 93 57 55 BmgBI CAC'GTC Cuts at: 791 791 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 2758 2839 3174 5320 2758 Size: 81 335 2146 2986 Fragments arranged by size: 2986 2146 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 354 3913 354 Size: 3559 1989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 194 628 634 691 733 833 2110 2170 194 Size: 434 6 57 42 100 1277 60 3572 Fragments arranged by size: 3572 1277 434 100 60 57 42 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1309 2476 2722 3601 4095 4393 1309 Size: 1167 246 879 494 298 2464 Fragments arranged by size: 2464 1167 879 494 298 246 BsaI GGTCTCn'nnnn_ Cuts at: 1705 5252 1705 Size: 3547 2001 BsaAI yAC'GTr Cuts at: 1019 1357 2538 2899 1019 Size: 338 1181 361 3668 Fragments arranged by size: 3668 1181 361 338 BsaBI GATnn'nnATC Cuts at: 6 3060 5099 6 Size: 3054 2039 455 Fragments arranged by size: 3054 2039 455 BsaHI Gr'CG_yC Cuts at: 663 919 1126 1663 1836 2977 3225 3929 5425 Size: 256 207 537 173 1141 248 704 1496 Cuts at: 5425 663 Size: 786 Fragments arranged by size: 1496 1141 786 704 537 256 248 207 173 BsaWI w'CCGG_w Cuts at: 1218 2735 3256 3936 4210 4357 4768 1218 Size: 1517 521 680 274 147 411 1998 Fragments arranged by size: 1998 1517 680 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1470 1500 2251 2281 2311 2341 1470 Size: 30 751 30 30 30 4677 Fragments arranged by size: 4677 751 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 440 631 688 1564 1879 1927 1990 2029 2059 Size: 191 57 876 315 48 63 39 30 Cuts at: 2059 2167 2317 440 Size: 108 150 3671 Fragments arranged by size: 3671 876 315 191 150 108 63 57 48 39 30 BseYI C'CCAG_C Cuts at: 150 168 1459 1567 1638 1677 1746 2385 2971 Size: 18 1291 108 71 39 69 639 586 Cuts at: 2971 3729 4308 4804 150 Size: 758 579 496 894 Fragments arranged by size: 1291 894 758 639 586 579 496 108 71 69 39 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 725 820 1152 1270 2314 2527 725 Size: 95 332 118 1044 213 3746 Fragments arranged by size: 3746 1044 332 213 118 95 BsiEI CG_ry'CG Cuts at: 104 978 1065 1234 2062 3088 3134 4344 104 Size: 874 87 169 828 1026 46 1210 1308 Fragments arranged by size: 1308 1210 1026 874 828 169 87 46 BsiHKAI G_wGCw'C Cuts at: 1154 1391 2560 3338 3528 4322 1154 Size: 237 1169 778 190 794 2380 Fragments arranged by size: 2380 1169 794 778 237 190 BsmI GAATG_Cn' Cuts at: 300 1910 300 Size: 1610 3938 Bsp1286I G_dGCh'C Cuts at: 337 433 834 1099 1154 1305 1362 1391 1567 Size: 96 401 265 55 151 57 29 176 Cuts at: 1567 1583 2309 2560 3171 3264 3338 3528 3590 Size: 16 726 251 611 93 74 190 62 Cuts at: 3590 4322 337 Size: 732 1563 Fragments arranged by size: 1563 732 726 611 401 265 251 190 176 151 96 93 74 62 57 55 29 16 BspEI T'CCGG_A Cuts at: 4768 4768 Size: 5548 BspHI T'CATG_A Cuts at: 2159 4724 2159 Size: 2565 2983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 336 2508 2596 2810 3112 3493 336 Size: 2172 88 214 302 381 2391 Fragments arranged by size: 2391 2172 381 302 214 88 BsrBI CCG'CTC Cuts at: 50 134 558 570 1216 1278 1875 1950 2025 Size: 84 424 12 646 62 597 75 75 Cuts at: 2025 2079 2094 2127 3838 3892 5378 5543 50 Size: 54 15 33 1711 54 1486 165 55 Fragments arranged by size: 1711 1486 646 597 424 165 84 75 75 62 55 54 54 33 15 12 BsrDI GCAATG_nn' Cuts at: 3458 3458 Size: 5548 BsrFI r'CCGG_y Cuts at: 659 665 1089 1168 1229 1245 1647 1659 1786 Size: 6 424 79 61 16 402 12 127 Cuts at: 1786 3544 3725 3936 659 Size: 1758 181 211 2271 Fragments arranged by size: 2271 1758 424 402 211 181 127 79 61 16 12 6 BssHII G'CGCG_C Cuts at: 1045 1045 Size: 5548 BssSI C'ACGA_G Cuts at: 1988 3817 4177 1988 Size: 1829 360 3359 Fragments arranged by size: 3359 1829 360 BstAPI GCAn_nnn'nTGC Cuts at: 2923 2923 Size: 5548 BstBI TT'CG_AA Cuts at: 110 110 Size: 5548 BstEII G'GTnAC_C Cuts at: 217 1764 217 Size: 1547 4001 BstXI CCAn_nnnn'nTGG Cuts at: 582 2401 582 Size: 1819 3729 BstYI r'GATC_y Cuts at: 1 2571 3038 3061 3396 3642 4656 4742 4754 Size: 2570 467 23 335 246 1014 86 12 Cuts at: 4754 5100 1 Size: 346 449 Fragments arranged by size: 2570 1014 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 339 339 Size: 5548 BtgI C'CryG_G Cuts at: 125 425 433 1706 125 Size: 300 8 1273 3967 Fragments arranged by size: 3967 1273 300 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3496 3677 3840 3496 Size: 181 163 5204 Fragments arranged by size: 5204 181 163 BtsI GCAGTG_nn' Cuts at: 488 605 961 1514 2789 488 Size: 117 356 553 1275 3247 Fragments arranged by size: 3247 1275 553 356 117 DraI TTT'AAA Cuts at: 2545 2545 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 1729 2175 3252 4112 1729 Size: 446 1077 860 3165 Fragments arranged by size: 3165 1077 860 446 EaeI y'GGCC_r Cuts at: 587 975 1231 1784 3131 3305 3696 3723 5070 Size: 388 256 553 1347 174 391 27 1347 Cuts at: 5070 587 Size: 1065 Fragments arranged by size: 1347 1347 1065 553 391 388 256 174 27 EagI C'GGCC_G Cuts at: 975 1231 3131 975 Size: 256 1900 3392 Fragments arranged by size: 3392 1900 256 EarI CTCTTCn'nnn_ Cuts at: 371 560 1291 2063 2158 3569 3779 371 Size: 189 731 772 95 1411 210 2140 Fragments arranged by size: 2140 1411 772 731 210 189 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1101 1359 1960 1972 2089 3754 4066 4212 5358 Size: 258 601 12 117 1665 312 146 1146 Cuts at: 5358 1101 Size: 1291 Fragments arranged by size: 1665 1291 1146 601 312 258 146 117 12 EcoICRI GAG'CTC Cuts at: 1389 2558 1389 Size: 1169 4379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 194 413 628 634 691 733 833 2110 2170 Size: 219 215 6 57 42 100 1277 60 Cuts at: 2170 3371 3803 4552 4820 194 Size: 1201 432 749 268 922 Fragments arranged by size: 1277 1201 922 749 432 268 219 215 100 60 57 42 6 EcoNI CCTnn'n_nnAGG Cuts at: 1007 1437 1007 Size: 430 5118 EcoO109I rG'GnC_Cy Cuts at: 185 409 830 831 1012 1579 2685 185 Size: 224 421 1 181 567 1106 3048 Fragments arranged by size: 3048 1106 567 421 224 181 1 EcoRI G'AATT_C Cuts at: 2550 2550 Size: 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3565 3565 Size: 5548 FspI TGC'GCA Cuts at: 3327 3327 Size: 5548 HaeII r_GCGC'y Cuts at: 634 660 666 756 816 1188 1335 1574 1666 Size: 26 6 90 60 372 147 239 92 Cuts at: 1666 1839 2980 3228 4252 5231 634 Size: 173 1141 248 1024 979 951 Fragments arranged by size: 1141 1024 979 951 372 248 239 173 147 92 90 60 26 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 909 941 1612 1644 2251 2283 3388 3418 3420 Size: 32 671 32 607 32 1105 30 2 Cuts at: 3420 3450 909 Size: 30 3007 Fragments arranged by size: 3007 1105 671 607 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2585 2585 Size: 5548 KasI G'GCGC_C Cuts at: 662 1662 1835 2976 3224 662 Size: 1000 173 1141 248 2986 Fragments arranged by size: 2986 1141 1000 248 173 KpnI G_GTAC'C Cuts at: 2566 2566 Size: 5548 MluI A'CGCG_T Cuts at: 4981 4981 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 499 518 795 4219 4403 499 Size: 19 277 3424 184 1644 Fragments arranged by size: 3424 1644 277 184 19 MscI TGG'CCA Cuts at: 3307 3307 Size: 5548 MslI CAynn'nnrTG Cuts at: 3535 3662 3535 Size: 127 5421 MspA1I CmG'CkG Cuts at: 172 726 879 1157 1708 1854 1920 2657 2971 Size: 554 153 278 551 146 66 737 314 Cuts at: 2971 3331 4346 4591 172 Size: 360 1015 245 1129 Fragments arranged by size: 1129 1015 737 554 551 360 314 278 245 153 146 66 NaeI GCC'GGC Cuts at: 661 667 1231 1661 1788 3727 661 Size: 6 564 430 127 1939 2482 Fragments arranged by size: 2482 1939 564 430 127 6 NarI GG'CG_CC Cuts at: 663 1663 1836 2977 3225 663 Size: 1000 173 1141 248 2986 Fragments arranged by size: 2986 1141 1000 248 173 NcoI C'CATG_G Cuts at: 125 425 125 Size: 300 5248 NgoMIV G'CCGG_C Cuts at: 659 665 1229 1659 1786 3725 659 Size: 6 564 430 127 1939 2482 Fragments arranged by size: 2482 1939 564 430 127 6 NruI TCG'CGA Cuts at: 3909 3909 Size: 5548 NspI r_CATG'y Cuts at: 2599 3099 4008 2599 Size: 500 909 4139 Fragments arranged by size: 4139 909 500 PasI CC'CwG_GG Cuts at: 1003 1465 2272 1003 Size: 462 807 4279 Fragments arranged by size: 4279 807 462 PciI A'CATG_T Cuts at: 4004 4004 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1433 1433 Size: 5548 PfoI T'CCnGG_A Cuts at: 650 849 650 Size: 199 5349 PmeI GTTT'AAAC Cuts at: 2545 2545 Size: 5548 PmlI CAC'GTG Cuts at: 1019 1357 1019 Size: 338 5210 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4687 4719 4687 Size: 32 5516 PpuMI rG'GwC_Cy Cuts at: 185 409 185 Size: 224 5324 PshAI GACnn'nnGTC Cuts at: 2435 2435 Size: 5548 PspOMI G'GGCC_C Cuts at: 429 830 1579 429 Size: 401 749 4398 Fragments arranged by size: 4398 749 401 PstI C_TGCA'G Cuts at: 878 1083 1886 2033 2282 2378 2593 3278 878 Size: 205 803 147 249 96 215 685 3148 Fragments arranged by size: 3148 803 685 249 215 205 147 96 PvuI CG_AT'CG Cuts at: 104 104 Size: 5548 PvuII CAG'CTG Cuts at: 172 879 1920 2971 3331 172 Size: 707 1041 1051 360 2389 Fragments arranged by size: 2389 1051 1041 707 360 RsrII CG'GwC_CG Cuts at: 3741 3741 Size: 5548 SacI G_AGCT'C Cuts at: 1391 2560 1391 Size: 1169 4379 SacII CC_GC'GG Cuts at: 1709 1709 Size: 5548 SalI G'TCGA_C Cuts at: 2583 2583 Size: 5548 SapI GCTCTTCn'nnn_ Cuts at: 560 3569 3779 560 Size: 3009 210 2329 Fragments arranged by size: 3009 2329 210 SbfI CC_TGCA'GG Cuts at: 2593 2593 Size: 5548 ScaI AGT'ACT Cuts at: 5065 5065 Size: 5548 SfcI C'TryA_G Cuts at: 33 874 1079 1718 1882 2029 2278 2374 2589 Size: 841 205 639 164 147 249 96 215 Cuts at: 2589 3274 4269 4460 33 Size: 685 995 191 1121 Fragments arranged by size: 1121 995 841 685 639 249 215 205 191 164 147 96 SfoI GGC'GCC Cuts at: 664 1664 1837 2978 3226 664 Size: 1000 173 1141 248 2986 Fragments arranged by size: 2986 1141 1000 248 173 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 659 1168 1245 1659 659 Size: 509 77 414 4548 Fragments arranged by size: 4548 509 414 77 SmaI CCC'GGG Cuts at: 1312 1846 1942 2568 1312 Size: 534 96 626 4292 Fragments arranged by size: 4292 626 534 96 SmlI C'TyrA_G Cuts at: 736 1324 2491 2701 3616 4110 4372 736 Size: 588 1167 210 915 494 262 1912 Fragments arranged by size: 1912 1167 915 588 494 262 210 SnaBI TAC'GTA Cuts at: 2538 2538 Size: 5548 SpeI A'CTAG_T Cuts at: 67 67 Size: 5548 SphI G_CATG'C Cuts at: 2599 3099 2599 Size: 500 5048 SrfI GCCC'GGGC Cuts at: 1942 1942 Size: 5548 StuI AGG'CCT Cuts at: 1240 1443 1240 Size: 203 5345 StyI C'CwwG_G Cuts at: 125 355 425 626 1403 1526 2680 3034 125 Size: 230 70 201 777 123 1154 354 2639 Fragments arranged by size: 2639 1154 777 354 230 201 123 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1814 2048 2255 3129 3262 3721 3932 1814 Size: 234 207 874 133 459 211 3430 Fragments arranged by size: 3430 874 459 234 211 207 133 TatI w'GTAC_w Cuts at: 5063 5063 Size: 5548 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1470 1502 2311 2343 5065 5097 1470 Size: 32 809 32 2722 32 1921 Fragments arranged by size: 2722 1921 809 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3343 3343 Size: 5548 XbaI T'CTAG_A Cuts at: 2577 2577 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1818 3041 1818 Size: 1223 4325 XhoI C'TCGA_G Cuts at: 736 736 Size: 5548 XmaI C'CCGG_G Cuts at: 1310 1844 1940 2566 1310 Size: 534 96 626 4292 Fragments arranged by size: 4292 626 534 96 XmnI GAAnn'nnTTC Cuts at: 2404 2404 Size: 5548 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AloI AscI BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI PacI PsiI PspXI PsrI SanDI SexAI SfiI SspI SwaI ZraI