Restriction map for pF1KSDA1187
 (Circular) (Six-base) MAPSORT of: pF1KSDA1187.seq  Check: 6824  from: 1  to: 5548



pF1KSDA1187  5548 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AccI GT'mk_AC

Cuts at:   2584    2584 
   Size:       5548
 
Acc65I G'GTAC_C

Cuts at:   2562    2562 
   Size:       5548
 
AclI AA'CG_TT

Cuts at:   5388    5388 
   Size:       5548
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    413    3371    3803    4552    4820     413 
   Size:       2958     432     749     268    1141

  Fragments arranged by size: 

                2958    1141     749     432     268
 
AfeI AGC'GCT

Cuts at:    754     814     754 
   Size:         60    5488
 
AflIII A'CryG_T

Cuts at:   2898    4004    4981    2898 
   Size:       1106     977    3465

  Fragments arranged by size: 

                3465    1106     977
 
AgeI A'CCGG_T

Cuts at:   3936    3936 
   Size:       5548
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    719    1460    2365     719 
   Size:        741     905    3902

  Fragments arranged by size: 

                3902     905     741
 
AlwNI CAG_nnn'CTG

Cuts at:   1341    1726    1753    2088    2245    2851    4420    1341 
   Size:        385      27     335     157     606    1569    2469

  Fragments arranged by size: 

                2469    1569     606     385     335     157      27
 
ApaI G_GGCC'C

Cuts at:    433     834    1583     433 
   Size:        401     749    4398

  Fragments arranged by size: 

                4398     749     401
 
ApaLI G'TGCA_C

Cuts at:   1150    4318    1150 
   Size:       3168    2380
 
ApoI r'AATT_y

Cuts at:   2550    4901    5157    2550 
   Size:       2351     256    2941

  Fragments arranged by size: 

                2941    2351     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5548
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5548
 
AvaI C'yCGr_G

Cuts at:    736    1310    1505    1582    1844    1940    2566     736 
   Size:        574     195      77     262      96     626    3718

  Fragments arranged by size: 

                3718     626     574     262     195      96      77
 
AvrII C'CTAG_G

Cuts at:    355     355 
   Size:       5548
 
BamHI G'GATC_C

Cuts at:   2571    2571 
   Size:       5548
 
BanI G'GyrC_C

Cuts at:    662    1510    1650    1662    1789    1835    2562    2976    3224 
   Size:        848     140      12     127      46     727     414     248

Cuts at:   3224    3259     662 
   Size:         35    2951

  Fragments arranged by size: 

                2951     848     727     414     248     140     127      46
                  35      12
 
BanII G_rGCy'C

Cuts at:    337     433     834    1391    1567    1583    2309    2560    3590 
   Size:         96     401     557     176      16     726     251    1030

Cuts at:   3590     337 
   Size:       2295

  Fragments arranged by size: 

                2295    1030     726     557     401     251     176      96
                  16
 
BbeI G_GCGC'C

Cuts at:    666    1666    1839    2980    3228     666 
   Size:       1000     173    1141     248    2986

  Fragments arranged by size: 

                2986    1141    1000     248     173
 
BbsI GAAGACnn'nnnn_

Cuts at:    793     793 
   Size:       5548
 
BciVI GTATCCnnnnn_n'

Cuts at:   3444    4207    5548    3444 
   Size:        763    1341    3444

  Fragments arranged by size: 

                3444    1341     763
 
BclI T'GATC_A

Cuts at:   3066    3066 
   Size:       5548
 
BglI GCCn_nnn'nGGC

Cuts at:    595    1042    1648     595 
   Size:        447     606    4495

  Fragments arranged by size: 

                4495     606     447
 
BglII A'GATC_T

Cuts at:   3061    5100    3061 
   Size:       2039    3509
 
BlpI GC'TnA_GC

Cuts at:    880    1390    2658     880 
   Size:        510    1268    3770

  Fragments arranged by size: 

                3770    1268     510
 
Bme1580I G_kGCm'C

Cuts at:    433     834    1099    1154    1305    1362    1583    3171    3264 
   Size:        401     265      55     151      57     221    1588      93

Cuts at:   3264    4322     433 
   Size:       1058    1659

  Fragments arranged by size: 

                1659    1588    1058     401     265     221     151      93
                  57      55
 
BmgBI CAC'GTC

Cuts at:    791     791 
   Size:       5548
 
BmrI ACTGGGnnnn_n'

Cuts at:   2758    2839    3174    5320    2758 
   Size:         81     335    2146    2986

  Fragments arranged by size: 

                2986    2146     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    354    3913     354 
   Size:       3559    1989
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    194     628     634     691     733     833    2110    2170     194 
   Size:        434       6      57      42     100    1277      60    3572

  Fragments arranged by size: 

                3572    1277     434     100      60      57      42       6
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1309    2476    2722    3601    4095    4393    1309 
   Size:       1167     246     879     494     298    2464

  Fragments arranged by size: 

                2464    1167     879     494     298     246
 
BsaI GGTCTCn'nnnn_

Cuts at:   1705    5252    1705 
   Size:       3547    2001
 
BsaAI yAC'GTr

Cuts at:   1019    1357    2538    2899    1019 
   Size:        338    1181     361    3668

  Fragments arranged by size: 

                3668    1181     361     338
 
BsaBI GATnn'nnATC

Cuts at:      6    3060    5099       6 
   Size:       3054    2039     455

  Fragments arranged by size: 

                3054    2039     455
 
BsaHI Gr'CG_yC

Cuts at:    663     919    1126    1663    1836    2977    3225    3929    5425 
   Size:        256     207     537     173    1141     248     704    1496

Cuts at:   5425     663 
   Size:        786

  Fragments arranged by size: 

                1496    1141     786     704     537     256     248     207
                 173
 
BsaWI w'CCGG_w

Cuts at:   1218    2735    3256    3936    4210    4357    4768    1218 
   Size:       1517     521     680     274     147     411    1998

  Fragments arranged by size: 

                1998    1517     680     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1470    1500    2251    2281    2311    2341    1470 
   Size:         30     751      30      30      30    4677

  Fragments arranged by size: 

                4677     751      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    440     631     688    1564    1879    1927    1990    2029    2059 
   Size:        191      57     876     315      48      63      39      30

Cuts at:   2059    2167    2317     440 
   Size:        108     150    3671

  Fragments arranged by size: 

                3671     876     315     191     150     108      63      57
                  48      39      30
 
BseYI C'CCAG_C

Cuts at:    150     168    1459    1567    1638    1677    1746    2385    2971 
   Size:         18    1291     108      71      39      69     639     586

Cuts at:   2971    3729    4308    4804     150 
   Size:        758     579     496     894

  Fragments arranged by size: 

                1291     894     758     639     586     579     496     108
                  71      69      39      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    725     820    1152    1270    2314    2527     725 
   Size:         95     332     118    1044     213    3746

  Fragments arranged by size: 

                3746    1044     332     213     118      95
 
BsiEI CG_ry'CG

Cuts at:    104     978    1065    1234    2062    3088    3134    4344     104 
   Size:        874      87     169     828    1026      46    1210    1308

  Fragments arranged by size: 

                1308    1210    1026     874     828     169      87      46
            
 
BsiHKAI G_wGCw'C

Cuts at:   1154    1391    2560    3338    3528    4322    1154 
   Size:        237    1169     778     190     794    2380

  Fragments arranged by size: 

                2380    1169     794     778     237     190
 
BsmI GAATG_Cn'

Cuts at:    300    1910     300 
   Size:       1610    3938
 
Bsp1286I G_dGCh'C

Cuts at:    337     433     834    1099    1154    1305    1362    1391    1567 
   Size:         96     401     265      55     151      57      29     176

Cuts at:   1567    1583    2309    2560    3171    3264    3338    3528    3590 
   Size:         16     726     251     611      93      74     190      62

Cuts at:   3590    4322     337 
   Size:        732    1563

  Fragments arranged by size: 

                1563     732     726     611     401     265     251     190
                 176     151      96      93      74      62      57      55
                  29      16
 
BspEI T'CCGG_A

Cuts at:   4768    4768 
   Size:       5548
 
BspHI T'CATG_A

Cuts at:   2159    4724    2159 
   Size:       2565    2983
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    336    2508    2596    2810    3112    3493     336 
   Size:       2172      88     214     302     381    2391

  Fragments arranged by size: 

                2391    2172     381     302     214      88
 
BsrBI CCG'CTC

Cuts at:     50     134     558     570    1216    1278    1875    1950    2025 
   Size:         84     424      12     646      62     597      75      75

Cuts at:   2025    2079    2094    2127    3838    3892    5378    5543      50 
   Size:         54      15      33    1711      54    1486     165      55

  Fragments arranged by size: 

                1711    1486     646     597     424     165      84      75
                  75      62      55      54      54      33      15      12
            
 
BsrDI GCAATG_nn'

Cuts at:   3458    3458 
   Size:       5548
 
BsrFI r'CCGG_y

Cuts at:    659     665    1089    1168    1229    1245    1647    1659    1786 
   Size:          6     424      79      61      16     402      12     127

Cuts at:   1786    3544    3725    3936     659 
   Size:       1758     181     211    2271

  Fragments arranged by size: 

                2271    1758     424     402     211     181     127      79
                  61      16      12       6
 
BssHII G'CGCG_C

Cuts at:   1045    1045 
   Size:       5548
 
BssSI C'ACGA_G

Cuts at:   1988    3817    4177    1988 
   Size:       1829     360    3359

  Fragments arranged by size: 

                3359    1829     360
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2923    2923 
   Size:       5548
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       5548
 
BstEII G'GTnAC_C

Cuts at:    217    1764     217 
   Size:       1547    4001
 
BstXI CCAn_nnnn'nTGG

Cuts at:    582    2401     582 
   Size:       1819    3729
 
BstYI r'GATC_y

Cuts at:      1    2571    3038    3061    3396    3642    4656    4742    4754 
   Size:       2570     467      23     335     246    1014      86      12

Cuts at:   4754    5100       1 
   Size:        346     449

  Fragments arranged by size: 

                2570    1014     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    339     339 
   Size:       5548
 
BtgI C'CryG_G

Cuts at:    125     425     433    1706     125 
   Size:        300       8    1273    3967

  Fragments arranged by size: 

                3967    1273     300       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3496    3677    3840    3496 
   Size:        181     163    5204

  Fragments arranged by size: 

                5204     181     163
 
BtsI GCAGTG_nn'

Cuts at:    488     605     961    1514    2789     488 
   Size:        117     356     553    1275    3247

  Fragments arranged by size: 

                3247    1275     553     356     117
 
DraI TTT'AAA

Cuts at:   2545    2545 
   Size:       5548
 
DrdI GACnn_nn'nnGTC

Cuts at:   1729    2175    3252    4112    1729 
   Size:        446    1077     860    3165

  Fragments arranged by size: 

                3165    1077     860     446
 
EaeI y'GGCC_r

Cuts at:    587     975    1231    1784    3131    3305    3696    3723    5070 
   Size:        388     256     553    1347     174     391      27    1347

Cuts at:   5070     587 
   Size:       1065

  Fragments arranged by size: 

                1347    1347    1065     553     391     388     256     174
                  27
 
EagI C'GGCC_G

Cuts at:    975    1231    3131     975 
   Size:        256    1900    3392

  Fragments arranged by size: 

                3392    1900     256
 
EarI CTCTTCn'nnn_

Cuts at:    371     560    1291    2063    2158    3569    3779     371 
   Size:        189     731     772      95    1411     210    2140

  Fragments arranged by size: 

                2140    1411     772     731     210     189      95
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1101    1359    1960    1972    2089    3754    4066    4212    5358 
   Size:        258     601      12     117    1665     312     146    1146

Cuts at:   5358    1101 
   Size:       1291

  Fragments arranged by size: 

                1665    1291    1146     601     312     258     146     117
                  12
 
EcoICRI GAG'CTC

Cuts at:   1389    2558    1389 
   Size:       1169    4379
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    194     413     628     634     691     733     833    2110    2170 
   Size:        219     215       6      57      42     100    1277      60

Cuts at:   2170    3371    3803    4552    4820     194 
   Size:       1201     432     749     268     922

  Fragments arranged by size: 

                1277    1201     922     749     432     268     219     215
                 100      60      57      42       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1007    1437    1007 
   Size:        430    5118
 
EcoO109I rG'GnC_Cy

Cuts at:    185     409     830     831    1012    1579    2685     185 
   Size:        224     421       1     181     567    1106    3048

  Fragments arranged by size: 

                3048    1106     567     421     224     181       1
 
EcoRI G'AATT_C

Cuts at:   2550    2550 
   Size:       5548
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3565    3565 
   Size:       5548
 
FspI TGC'GCA

Cuts at:   3327    3327 
   Size:       5548
 
HaeII r_GCGC'y

Cuts at:    634     660     666     756     816    1188    1335    1574    1666 
   Size:         26       6      90      60     372     147     239      92

Cuts at:   1666    1839    2980    3228    4252    5231     634 
   Size:        173    1141     248    1024     979     951

  Fragments arranged by size: 

                1141    1024     979     951     372     248     239     173
                 147      92      90      60      26       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    909     941    1612    1644    2251    2283    3388    3418    3420 
   Size:         32     671      32     607      32    1105      30       2

Cuts at:   3420    3450     909 
   Size:         30    3007

  Fragments arranged by size: 

                3007    1105     671     607      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2585    2585 
   Size:       5548
 
KasI G'GCGC_C

Cuts at:    662    1662    1835    2976    3224     662 
   Size:       1000     173    1141     248    2986

  Fragments arranged by size: 

                2986    1141    1000     248     173
 
KpnI G_GTAC'C

Cuts at:   2566    2566 
   Size:       5548
 
MluI A'CGCG_T

Cuts at:   4981    4981 
   Size:       5548
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    499     518     795    4219    4403     499 
   Size:         19     277    3424     184    1644

  Fragments arranged by size: 

                3424    1644     277     184      19
 
MscI TGG'CCA

Cuts at:   3307    3307 
   Size:       5548
 
MslI CAynn'nnrTG

Cuts at:   3535    3662    3535 
   Size:        127    5421
 
MspA1I CmG'CkG

Cuts at:    172     726     879    1157    1708    1854    1920    2657    2971 
   Size:        554     153     278     551     146      66     737     314

Cuts at:   2971    3331    4346    4591     172 
   Size:        360    1015     245    1129

  Fragments arranged by size: 

                1129    1015     737     554     551     360     314     278
                 245     153     146      66
 
NaeI GCC'GGC

Cuts at:    661     667    1231    1661    1788    3727     661 
   Size:          6     564     430     127    1939    2482

  Fragments arranged by size: 

                2482    1939     564     430     127       6
 
NarI GG'CG_CC

Cuts at:    663    1663    1836    2977    3225     663 
   Size:       1000     173    1141     248    2986

  Fragments arranged by size: 

                2986    1141    1000     248     173
 
NcoI C'CATG_G

Cuts at:    125     425     125 
   Size:        300    5248
 
NgoMIV G'CCGG_C

Cuts at:    659     665    1229    1659    1786    3725     659 
   Size:          6     564     430     127    1939    2482

  Fragments arranged by size: 

                2482    1939     564     430     127       6
 
NruI TCG'CGA

Cuts at:   3909    3909 
   Size:       5548
 
NspI r_CATG'y

Cuts at:   2599    3099    4008    2599 
   Size:        500     909    4139

  Fragments arranged by size: 

                4139     909     500
 
PasI CC'CwG_GG

Cuts at:   1003    1465    2272    1003 
   Size:        462     807    4279

  Fragments arranged by size: 

                4279     807     462
 
PciI A'CATG_T

Cuts at:   4004    4004 
   Size:       5548
 
PflMI CCAn_nnn'nTGG

Cuts at:   1433    1433 
   Size:       5548
 
PfoI T'CCnGG_A

Cuts at:    650     849     650 
   Size:        199    5349
 
PmeI GTTT'AAAC

Cuts at:   2545    2545 
   Size:       5548
 
PmlI CAC'GTG

Cuts at:   1019    1357    1019 
   Size:        338    5210
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4687    4719    4687 
   Size:         32    5516
 
PpuMI rG'GwC_Cy

Cuts at:    185     409     185 
   Size:        224    5324
 
PshAI GACnn'nnGTC

Cuts at:   2435    2435 
   Size:       5548
 
PspOMI G'GGCC_C

Cuts at:    429     830    1579     429 
   Size:        401     749    4398

  Fragments arranged by size: 

                4398     749     401
 
PstI C_TGCA'G

Cuts at:    878    1083    1886    2033    2282    2378    2593    3278     878 
   Size:        205     803     147     249      96     215     685    3148

  Fragments arranged by size: 

                3148     803     685     249     215     205     147      96
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5548
 
PvuII CAG'CTG

Cuts at:    172     879    1920    2971    3331     172 
   Size:        707    1041    1051     360    2389

  Fragments arranged by size: 

                2389    1051    1041     707     360
 
RsrII CG'GwC_CG

Cuts at:   3741    3741 
   Size:       5548
 
SacI G_AGCT'C

Cuts at:   1391    2560    1391 
   Size:       1169    4379
 
SacII CC_GC'GG

Cuts at:   1709    1709 
   Size:       5548
 
SalI G'TCGA_C

Cuts at:   2583    2583 
   Size:       5548
 
SapI GCTCTTCn'nnn_

Cuts at:    560    3569    3779     560 
   Size:       3009     210    2329

  Fragments arranged by size: 

                3009    2329     210
 
SbfI CC_TGCA'GG

Cuts at:   2593    2593 
   Size:       5548
 
ScaI AGT'ACT

Cuts at:   5065    5065 
   Size:       5548
 
SfcI C'TryA_G

Cuts at:     33     874    1079    1718    1882    2029    2278    2374    2589 
   Size:        841     205     639     164     147     249      96     215

Cuts at:   2589    3274    4269    4460      33 
   Size:        685     995     191    1121

  Fragments arranged by size: 

                1121     995     841     685     639     249     215     205
                 191     164     147      96
 
SfoI GGC'GCC

Cuts at:    664    1664    1837    2978    3226     664 
   Size:       1000     173    1141     248    2986

  Fragments arranged by size: 

                2986    1141    1000     248     173
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5548
 
SgrAI Cr'CCGG_yG

Cuts at:    659    1168    1245    1659     659 
   Size:        509      77     414    4548

  Fragments arranged by size: 

                4548     509     414      77
 
SmaI CCC'GGG

Cuts at:   1312    1846    1942    2568    1312 
   Size:        534      96     626    4292

  Fragments arranged by size: 

                4292     626     534      96
 
SmlI C'TyrA_G

Cuts at:    736    1324    2491    2701    3616    4110    4372     736 
   Size:        588    1167     210     915     494     262    1912

  Fragments arranged by size: 

                1912    1167     915     588     494     262     210
 
SnaBI TAC'GTA

Cuts at:   2538    2538 
   Size:       5548
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5548
 
SphI G_CATG'C

Cuts at:   2599    3099    2599 
   Size:        500    5048
 
SrfI GCCC'GGGC

Cuts at:   1942    1942 
   Size:       5548
 
StuI AGG'CCT

Cuts at:   1240    1443    1240 
   Size:        203    5345
 
StyI C'CwwG_G

Cuts at:    125     355     425     626    1403    1526    2680    3034     125 
   Size:        230      70     201     777     123    1154     354    2639

  Fragments arranged by size: 

                2639    1154     777     354     230     201     123      70
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1814    2048    2255    3129    3262    3721    3932    1814 
   Size:        234     207     874     133     459     211    3430

  Fragments arranged by size: 

                3430     874     459     234     211     207     133
 
TatI w'GTAC_w

Cuts at:   5063    5063 
   Size:       5548
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1470    1502    2311    2343    5065    5097    1470 
   Size:         32     809      32    2722      32    1921

  Fragments arranged by size: 

                2722    1921     809      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3343    3343 
   Size:       5548
 
XbaI T'CTAG_A

Cuts at:   2577    2577 
   Size:       5548
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1818    3041    1818 
   Size:       1223    4325
 
XhoI C'TCGA_G

Cuts at:    736     736 
   Size:       5548
 
XmaI C'CCGG_G

Cuts at:   1310    1844    1940    2566    1310 
   Size:        534      96     626    4292

  Fragments arranged by size: 

                4292     626     534      96
 
XmnI GAAnn'nnTTC

Cuts at:   2404    2404 
   Size:       5548
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AloI     AscI     BaeI
    BbvCI     BcgI    BfrBI     BmtI   Bpu10I    BsiWI    BsmBI    BsrGI
  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NsiI     PacI     PsiI
    PspXI     PsrI    SanDI    SexAI     SfiI     SspI     SwaI     ZraI
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