Restriction map for
pF1KSDA1194
(Circular) (Six-base) MAPSORT of: pF1KSDA1194.seq Check: 3890 from: 1 to: 4810
pF1KSDA1194 4810 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 169 1846 169
Size: 1677 3133
Acc65I G'GTAC_C
Cuts at: 1824 1824
Size: 4810
AclI AA'CG_TT
Cuts at: 4650 4650
Size: 4810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 823 897 1085 1455 2633 3065 3814 4082
Size: 512 74 188 370 1178 432 749 268
Cuts at: 4082 311
Size: 1039
Fragments arranged by size:
1178 1039 749 512 432 370 268 188
74
AflII C'TTAA_G
Cuts at: 252 252
Size: 4810
AflIII A'CryG_T
Cuts at: 960 2160 3266 4243 960
Size: 1200 1106 977 1527
Fragments arranged by size:
1527 1200 1106 977
AgeI A'CCGG_T
Cuts at: 3198 3198
Size: 4810
AlwNI CAG_nnn'CTG
Cuts at: 604 1381 1688 2113 3682 604
Size: 777 307 425 1569 1732
Fragments arranged by size:
1732 1569 777 425 307
ApaI G_GGCC'C
Cuts at: 1648 1648
Size: 4810
ApaLI G'TGCA_C
Cuts at: 3580 3580
Size: 4810
ApoI r'AATT_y
Cuts at: 320 380 997 1027 1050 1327 1812 4163 4419
Size: 60 617 30 23 277 485 2351 256
Cuts at: 4419 320
Size: 711
Fragments arranged by size:
2351 711 617 485 277 256 60 30
23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4810
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4810
AvaI C'yCGr_G
Cuts at: 1828 1828
Size: 4810
BamHI G'GATC_C
Cuts at: 1833 1833
Size: 4810
BanI G'GyrC_C
Cuts at: 1824 2238 2486 2521 1824
Size: 414 248 35 4113
Fragments arranged by size:
4113 414 248 35
BanII G_rGCy'C
Cuts at: 149 431 1648 1745 1822 2852 149
Size: 282 1217 97 77 1030 2107
Fragments arranged by size:
2107 1217 1030 282 97 77
BbeI G_GCGC'C
Cuts at: 2242 2490 2242
Size: 248 4562
BbsI GAAGACnn'nnnn_
Cuts at: 173 829 173
Size: 656 4154
BbvCI CC'TCA_GC
Cuts at: 1619 1619
Size: 4810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1128 1162 1128
Size: 34 4776
BciVI GTATCCnnnnn_n'
Cuts at: 2706 3469 4810 2706
Size: 763 1341 2706
Fragments arranged by size:
2706 1341 763
BclI T'GATC_A
Cuts at: 2328 2328
Size: 4810
BfrBI ATG'CAT
Cuts at: 1212 1212
Size: 4810
BglII A'GATC_T
Cuts at: 1359 2323 4362 1359
Size: 964 2039 1807
Fragments arranged by size:
2039 1807 964
BlpI GC'TnA_GC
Cuts at: 1920 1920
Size: 4810
Bme1580I G_kGCm'C
Cuts at: 1648 2433 2526 3584 1648
Size: 785 93 1058 2874
Fragments arranged by size:
2874 1058 785 93
BmrI ACTGGGnnnn_n'
Cuts at: 225 783 2020 2101 2436 4582 225
Size: 558 1237 81 335 2146 453
Fragments arranged by size:
2146 1237 558 453 335 81
BmtI G_CTAG'C
Cuts at: 1042 1042
Size: 4810
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 4810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 1759
Size: 4810
Bpu10I CC'TnA_GC
Cuts at: 1619 1619
Size: 4810
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1984 2863 3357 3655 831
Size: 1153 879 494 298 1986
Fragments arranged by size:
1986 1153 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4514 4514
Size: 4810
BsaAI yAC'GTr
Cuts at: 1800 2161 1800
Size: 361 4449
BsaBI GATnn'nnATC
Cuts at: 6 2322 4361 6
Size: 2316 2039 455
Fragments arranged by size:
2316 2039 455
BsaHI Gr'CG_yC
Cuts at: 2239 2487 3191 4687 2239
Size: 248 704 1496 2362
Fragments arranged by size:
2362 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1997 2518 3198 3472 3619 4030 1997
Size: 521 680 274 147 411 2777
Fragments arranged by size:
2777 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1432 1462 1432
Size: 30 4780
BseYI C'CCAG_C
Cuts at: 471 2233 2991 3570 4066 471
Size: 1762 758 579 496 1215
Fragments arranged by size:
1762 1215 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1376
Size: 4810
BsiEI CG_ry'CG
Cuts at: 104 2350 2396 3606 104
Size: 2246 46 1210 1308
Fragments arranged by size:
2246 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 431 1405 1745 1822 2600 2790 3584 431
Size: 974 340 77 778 190 794 1657
Fragments arranged by size:
1657 974 794 778 340 190 77
Bsp1286I G_dGCh'C
Cuts at: 149 431 1405 1648 1745 1822 2433 2526 2600
Size: 282 974 243 97 77 611 93 74
Cuts at: 2600 2790 2852 3584 149
Size: 190 62 732 1375
Fragments arranged by size:
1375 974 732 611 282 243 190 97
93 77 74 62
BspEI T'CCGG_A
Cuts at: 4030 4030
Size: 4810
BspHI T'CATG_A
Cuts at: 1085 3986 1085
Size: 2901 1909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1858 2072 2374 2755 1858
Size: 214 302 381 3913
Fragments arranged by size:
3913 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3100 3154 4640 4805 50
Size: 3050 54 1486 165 55
Fragments arranged by size:
3050 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 825 2720 332
Size: 493 1895 2422
Fragments arranged by size:
2422 1895 493
BsrFI r'CCGG_y
Cuts at: 2806 2987 3198 2806
Size: 181 211 4418
Fragments arranged by size:
4418 211 181
BssSI C'ACGA_G
Cuts at: 3079 3439 3079
Size: 360 4450
BstAPI GCAn_nnn'nTGC
Cuts at: 2185 2185
Size: 4810
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4810
BstXI CCAn_nnnn'nTGG
Cuts at: 644 1320 1786 644
Size: 676 466 3668
Fragments arranged by size:
3668 676 466
BstYI r'GATC_y
Cuts at: 1 1359 1833 2300 2323 2658 2904 3918 4004
Size: 1358 474 467 23 335 246 1014 86
Cuts at: 4004 4016 4362 1
Size: 12 346 449
Fragments arranged by size:
1358 1014 474 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 170 170
Size: 4810
Bsu36I CC'TnA_GG
Cuts at: 1632 1632
Size: 4810
BtgI C'CryG_G
Cuts at: 1043 1043
Size: 4810
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2758 2939 3102 2758
Size: 181 163 4466
Fragments arranged by size:
4466 181 163
BtsI GCAGTG_nn'
Cuts at: 2051 2051
Size: 4810
ClaI AT'CG_AT
Cuts at: 1138 1138
Size: 4810
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1195 1230 1195
Size: 35 4775
DraI TTT'AAA
Cuts at: 437 1807 437
Size: 1370 3440
DrdI GACnn_nn'nnGTC
Cuts at: 2514 3374 2514
Size: 860 3950
EaeI y'GGCC_r
Cuts at: 2393 2567 2958 2985 4332 2393
Size: 174 391 27 1347 2871
Fragments arranged by size:
2871 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2393 2393
Size: 4810
EarI CTCTTCn'nnn_
Cuts at: 2831 3041 2831
Size: 210 4600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3016 3328 3474 4620 3016
Size: 312 146 1146 3206
Fragments arranged by size:
3206 1146 312 146
EcoICRI GAG'CTC
Cuts at: 429 1743 1820 429
Size: 1314 77 3419
Fragments arranged by size:
3419 1314 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 823 897 1085 1455 1759 2633 3065 3814
Size: 512 74 188 370 304 874 432 749
Cuts at: 3814 4082 311
Size: 268 1039
Fragments arranged by size:
1039 874 749 512 432 370 304 268
188 74
EcoNI CCTnn'n_nnAGG
Cuts at: 523 544 523
Size: 21 4789
EcoO109I rG'GnC_Cy
Cuts at: 1218 1645 1947 1218
Size: 427 302 4081
Fragments arranged by size:
4081 427 302
EcoRI G'AATT_C
Cuts at: 320 1812 320
Size: 1492 3318
EcoRV GAT'ATC
Cuts at: 828 828
Size: 4810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 278 2827 278
Size: 2549 2261
FspI TGC'GCA
Cuts at: 2589 2589
Size: 4810
HaeII r_GCGC'y
Cuts at: 769 2242 2490 3514 4493 769
Size: 1473 248 1024 979 1086
Fragments arranged by size:
1473 1086 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 580 612 1279 1311 1705 1737 2650
Size: 32 193 32 667 32 394 32 913
Cuts at: 2650 2680 2682 2712 355
Size: 30 2 30 2453
Fragments arranged by size:
2453 913 667 394 193 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1770 1847 1770
Size: 77 4733
HindIII A'AGCT_T
Cuts at: 249 340 249
Size: 91 4719
KasI G'GCGC_C
Cuts at: 2238 2486 2238
Size: 248 4562
KpnI G_GTAC'C
Cuts at: 1828 1828
Size: 4810
MluI A'CGCG_T
Cuts at: 4243 4243
Size: 4810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3481 3665 3481
Size: 184 4626
MscI TGG'CCA
Cuts at: 2569 2569
Size: 4810
MslI CAynn'nnrTG
Cuts at: 264 299 965 2797 2924 264
Size: 35 666 1832 127 2150
Fragments arranged by size:
2150 1832 666 127 35
MspA1I CmG'CkG
Cuts at: 1475 1623 1919 2233 2593 3608 3853 1475
Size: 148 296 314 360 1015 245 2432
Fragments arranged by size:
2432 1015 360 314 296 245 148
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 4810
NarI GG'CG_CC
Cuts at: 2239 2487 2239
Size: 248 4562
NcoI C'CATG_G
Cuts at: 1043 1043
Size: 4810
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 4810
NheI G'CTAG_C
Cuts at: 1038 1038
Size: 4810
NruI TCG'CGA
Cuts at: 3171 3171
Size: 4810
NsiI A_TGCA'T
Cuts at: 1214 1214
Size: 4810
NspI r_CATG'y
Cuts at: 964 1212 1861 2361 3270 964
Size: 248 649 500 909 2504
Fragments arranged by size:
2504 909 649 500 248
PciI A'CATG_T
Cuts at: 960 3266 960
Size: 2306 2504
PflMI CCAn_nnn'nTGG
Cuts at: 1168 1381 1665 1168
Size: 213 284 4313
Fragments arranged by size:
4313 284 213
PmeI GTTT'AAAC
Cuts at: 1807 1807
Size: 4810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3949 3981 3949
Size: 32 4778
PpuMI rG'GwC_Cy
Cuts at: 1218 1218
Size: 4810
PspOMI G'GGCC_C
Cuts at: 1644 1644
Size: 4810
PstI C_TGCA'G
Cuts at: 1855 2540 1855
Size: 685 4125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4810
PvuII CAG'CTG
Cuts at: 1475 1623 2233 2593 1475
Size: 148 610 360 3692
Fragments arranged by size:
3692 610 360 148
RsrII CG'GwC_CG
Cuts at: 3003 3003
Size: 4810
SacI G_AGCT'C
Cuts at: 431 1745 1822 431
Size: 1314 77 3419
Fragments arranged by size:
3419 1314 77
SalI G'TCGA_C
Cuts at: 1845 1845
Size: 4810
SanDI GG'GwC_CC
Cuts at: 1218 1218
Size: 4810
SapI GCTCTTCn'nnn_
Cuts at: 2831 3041 2831
Size: 210 4600
SbfI CC_TGCA'GG
Cuts at: 1855 1855
Size: 4810
ScaI AGT'ACT
Cuts at: 1115 4327 1115
Size: 3212 1598
SfcI C'TryA_G
Cuts at: 33 780 1851 2536 3531 3722 33
Size: 747 1071 685 995 191 1121
Fragments arranged by size:
1121 1071 995 747 685 191
SfoI GGC'GCC
Cuts at: 2240 2488 2240
Size: 248 4562
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4810
SmaI CCC'GGG
Cuts at: 1830 1830
Size: 4810
SmlI C'TyrA_G
Cuts at: 252 810 1963 2878 3372 3634 252
Size: 558 1153 915 494 262 1428
Fragments arranged by size:
1428 1153 915 558 494 262
SnaBI TAC'GTA
Cuts at: 1800 1800
Size: 4810
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4810
SphI G_CATG'C
Cuts at: 1861 2361 1861
Size: 500 4310
SspI AAT'ATT
Cuts at: 980 1396 980
Size: 416 4394
StuI AGG'CCT
Cuts at: 503 503
Size: 4810
StyI C'CwwG_G
Cuts at: 637 1043 1942 2296 637
Size: 406 899 354 3151
Fragments arranged by size:
3151 899 406 354
SwaI ATTT'AAAT
Cuts at: 437 437
Size: 4810
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2391 2524 2983 3194 2391
Size: 133 459 211 4007
Fragments arranged by size:
4007 459 211 133
TatI w'GTAC_w
Cuts at: 1113 4325 1113
Size: 3212 1598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4327 4359 4327
Size: 32 4778
Tth111I GACn'n_nGTC
Cuts at: 939 2605 939
Size: 1666 3144
XbaI T'CTAG_A
Cuts at: 925 1839 925
Size: 914 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2303 2303
Size: 4810
XmaI C'CCGG_G
Cuts at: 1828 1828
Size: 4810
XmnI GAAnn'nnTTC
Cuts at: 324 324
Size: 4810
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NsiI NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BglI BmgBI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII DraIII FseI FspAI HpaI MfeI
NdeI NotI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI XhoI
ZraI