Restriction map for
pF1KSDA1197
(Circular) (Six-base) MAPSORT of: pF1KSDA1197.seq Check: 803 from: 1 to: 7405
pF1KSDA1197 7405 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1638 3390 1638
Size: 1752 5653
AccI GT'mk_AC
Cuts at: 4441 4441
Size: 7405
Acc65I G'GTAC_C
Cuts at: 2082 4419 2082
Size: 2337 5068
AclI AA'CG_TT
Cuts at: 2341 7245 2341
Size: 4904 2501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1313 2414 3062 4228 5228 5660 6409 6677
Size: 247 1101 648 1166 1000 432 749 268
Cuts at: 6677 1066
Size: 1794
Fragments arranged by size:
1794 1166 1101 1000 749 648 432 268
247
AflIII A'CryG_T
Cuts at: 427 4175 4755 5861 6838 427
Size: 3748 580 1106 977 994
Fragments arranged by size:
3748 1106 994 977 580
AgeI A'CCGG_T
Cuts at: 5793 5793
Size: 7405
AleI CACnn'nnGTG
Cuts at: 1888 1888
Size: 7405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1440 1610 1440
Size: 170 7235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4066 4098 4066
Size: 32 7373
AlwNI CAG_nnn'CTG
Cuts at: 327 1942 2148 2664 3439 4108 4708 6277 327
Size: 1615 206 516 775 669 600 1569 1455
Fragments arranged by size:
1615 1569 1455 775 669 600 516 206
ApaLI G'TGCA_C
Cuts at: 6175 6175
Size: 7405
ApoI r'AATT_y
Cuts at: 219 466 1745 2379 4111 4407 6758 7014 219
Size: 247 1279 634 1732 296 2351 256 610
Fragments arranged by size:
2351 1732 1279 634 610 296 256 247
AseI AT'TA_AT
Cuts at: 20 866 1610 3613 20
Size: 846 744 2003 3812
Fragments arranged by size:
3812 2003 846 744
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7405
AvaI C'yCGr_G
Cuts at: 227 3907 3988 4423 227
Size: 3680 81 435 3209
Fragments arranged by size:
3680 3209 435 81
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 425 458 1250 1283 425
Size: 33 792 33 6547
Fragments arranged by size:
6547 792 33 33
BamHI G'GATC_C
Cuts at: 2317 4428 2317
Size: 2111 5294
BanI G'GyrC_C
Cuts at: 2082 2530 3812 4419 4833 5081 5116 2082
Size: 448 1282 607 414 248 35 4371
Fragments arranged by size:
4371 1282 607 448 414 248 35
BanII G_rGCy'C
Cuts at: 3908 4417 5447 3908
Size: 509 1030 5866
Fragments arranged by size:
5866 1030 509
BbeI G_GCGC'C
Cuts at: 4837 5085 4837
Size: 248 7157
BbsI GAAGACnn'nnnn_
Cuts at: 391 1139 2920 3053 3460 391
Size: 748 1781 133 407 4336
Fragments arranged by size:
4336 1781 748 407 133
BbvCI CC'TCA_GC
Cuts at: 4015 4015
Size: 7405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3318 3352 3318
Size: 34 7371
BciVI GTATCCnnnnn_n'
Cuts at: 657 3228 4255 5301 6064 7405 657
Size: 2571 1027 1046 763 1341 657
Fragments arranged by size:
2571 1341 1046 1027 763 657
BclI T'GATC_A
Cuts at: 1892 4923 1892
Size: 3031 4374
BglI GCCn_nnn'nGGC
Cuts at: 2374 2374
Size: 7405
BglII A'GATC_T
Cuts at: 1694 4918 6957 1694
Size: 3224 2039 2142
Fragments arranged by size:
3224 2142 2039
BlpI GC'TnA_GC
Cuts at: 2258 2508 4515 2258
Size: 250 2007 5148
Fragments arranged by size:
5148 2007 250
Bme1580I G_kGCm'C
Cuts at: 1931 2707 3916 5028 5121 6179 1931
Size: 776 1209 1112 93 1058 3157
Fragments arranged by size:
3157 1209 1112 1058 776 93
BmgBI CAC'GTC
Cuts at: 3173 3882 3173
Size: 709 6696
BmrI ACTGGGnnnn_n'
Cuts at: 1204 1664 2040 2768 2791 4615 4696 5031 7177
Size: 460 376 728 23 1824 81 335 2146
Cuts at: 7177 1204
Size: 1432
Fragments arranged by size:
2146 1824 1432 728 460 376 335 81
23
BmtI G_CTAG'C
Cuts at: 1289 1289
Size: 7405
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5770 5770
Size: 7405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 2051 2482 2666 3225 3335 3395 3416 3772
Size: 495 431 184 559 110 60 21 356
Cuts at: 3772 3976 1556
Size: 204 4985
Fragments arranged by size:
4985 559 495 431 356 204 184 110
60 21
Bpu10I CC'TnA_GC
Cuts at: 4015 4015
Size: 7405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2671 4579 5458 5952 6250 2444
Size: 227 1908 879 494 298 3599
Fragments arranged by size:
3599 1908 879 494 298 227
BsaI GGTCTCn'nnnn_
Cuts at: 633 1719 7109 633
Size: 1086 5390 929
Fragments arranged by size:
5390 1086 929
BsaAI yAC'GTr
Cuts at: 1581 1926 4395 4756 1581
Size: 345 2469 361 4230
Fragments arranged by size:
4230 2469 361 345
BsaBI GATnn'nnATC
Cuts at: 6 1030 4042 4141 4917 6956 6
Size: 1024 3012 99 776 2039 455
Fragments arranged by size:
3012 2039 1024 776 455 99
BsaHI Gr'CG_yC
Cuts at: 4834 5082 5786 7282 4834
Size: 248 704 1496 4957
Fragments arranged by size:
4957 1496 704 248
BsaWI w'CCGG_w
Cuts at: 786 3220 3837 4592 5113 5793 6067 6214 6625
Size: 2434 617 755 521 680 274 147 411
Cuts at: 6625 786
Size: 1566
Fragments arranged by size:
2434 1566 755 680 617 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1554 1584 1681 1711 1554
Size: 30 97 30 7248
Fragments arranged by size:
7248 97 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2552 2555 2558 2561 2564 2567 2570 2573 2612
Size: 3 3 3 3 3 3 3 39
Cuts at: 2612 2615 2618 2633 3918 4012 4015 2552
Size: 3 3 15 1285 94 3 5942
Fragments arranged by size:
5942 1285 94 39 15 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 859 968 1281 3280 3421 3534 4828 5586 6165
Size: 109 313 1999 141 113 1294 758 579
Cuts at: 6165 6661 859
Size: 496 1603
Fragments arranged by size:
1999 1603 1294 758 579 496 313 141
113 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 2287 2786 3014 3343 262
Size: 2025 499 228 329 4324
Fragments arranged by size:
4324 2025 499 329 228
BsiEI CG_ry'CG
Cuts at: 104 4945 4991 6201 104
Size: 4841 46 1210 1308
Fragments arranged by size:
4841 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 4417 5195 5385 6179 4417
Size: 778 190 794 5643
Fragments arranged by size:
5643 794 778 190
BsmI GAATG_Cn'
Cuts at: 711 711
Size: 7405
BsmBI CGTCTCn'nnnn_
Cuts at: 1084 1084
Size: 7405
Bsp1286I G_dGCh'C
Cuts at: 1931 2707 3908 3916 4417 5028 5121 5195 5385
Size: 776 1201 8 501 611 93 74 190
Cuts at: 5385 5447 6179 1931
Size: 62 732 3157
Fragments arranged by size:
3157 1201 776 732 611 501 190 93
74 62 8
BspEI T'CCGG_A
Cuts at: 786 3220 6625 786
Size: 2434 3405 1566
Fragments arranged by size:
3405 2434 1566
BspHI T'CATG_A
Cuts at: 6581 6581
Size: 7405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 385 1638 3145 3390 3971 4097 4453 4667 4969
Size: 1253 1507 245 581 126 356 214 302
Cuts at: 4969 5350 385
Size: 381 2440
Fragments arranged by size:
2440 1507 1253 581 381 356 302 245
214 126
BsrBI CCG'CTC
Cuts at: 50 5695 5749 7235 7400 50
Size: 5645 54 1486 165 55
Fragments arranged by size:
5645 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 771 1546 3725 5315 771
Size: 775 2179 1590 2861
Fragments arranged by size:
2861 2179 1590 775
BsrFI r'CCGG_y
Cuts at: 1597 5401 5582 5793 1597
Size: 3804 181 211 3209
Fragments arranged by size:
3804 3209 211 181
BssSI C'ACGA_G
Cuts at: 3178 5674 6034 3178
Size: 2496 360 4549
Fragments arranged by size:
4549 2496 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3442 4780 3442
Size: 1338 6067
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7405
BstXI CCAn_nnnn'nTGG
Cuts at: 2364 2740 2364
Size: 376 7029
BstYI r'GATC_y
Cuts at: 1 1694 2144 2317 4428 4895 4918 5253 5499
Size: 1693 450 173 2111 467 23 335 246
Cuts at: 5499 6513 6599 6611 6957 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2111 1693 1014 467 450 449 346 335
246 173 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1838 3853 1838
Size: 2015 5390
BtgI C'CryG_G
Cuts at: 1389 1700 2868 3005 3128 4157 4187 1389
Size: 311 1168 137 123 1029 30 4607
Fragments arranged by size:
4607 1168 1029 311 137 123 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1099 3786 5353 5534 5697 1099
Size: 2687 1567 181 163 2807
Fragments arranged by size:
2807 2687 1567 181 163
BtsI GCAGTG_nn'
Cuts at: 1592 2574 3515 3617 4646 1592
Size: 982 941 102 1029 4351
Fragments arranged by size:
4351 1029 982 941 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 740 775 3641 3676 740
Size: 35 2866 35 4469
Fragments arranged by size:
4469 2866 35 35
DraI TTT'AAA
Cuts at: 4402 4402
Size: 7405
DrdI GACnn_nn'nnGTC
Cuts at: 5109 5969 5109
Size: 860 6545
EaeI y'GGCC_r
Cuts at: 2139 2366 2718 4988 5162 5553 5580 6927 2139
Size: 227 352 2270 174 391 27 1347 2617
Fragments arranged by size:
2617 2270 1347 391 352 227 174 27
EagI C'GGCC_G
Cuts at: 4988 4988
Size: 7405
EarI CTCTTCn'nnn_
Cuts at: 957 2172 2305 4055 5426 5636 957
Size: 1215 133 1750 1371 210 2726
Fragments arranged by size:
2726 1750 1371 1215 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5611 5923 6069 7215 5611
Size: 312 146 1146 5801
Fragments arranged by size:
5801 1146 312 146
EcoICRI GAG'CTC
Cuts at: 4415 4415
Size: 7405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1313 1556 2051 2414 2482 2666 3062 3225
Size: 247 243 495 363 68 184 396 163
Cuts at: 3225 3335 3395 3416 3772 3976 4228 5228 5660
Size: 110 60 21 356 204 252 1000 432
Cuts at: 5660 6409 6677 1066
Size: 749 268 1794
Fragments arranged by size:
1794 1000 749 495 432 396 363 356
268 252 247 243 204 184 163 110
68 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1862 2033 3490 4243 1862
Size: 171 1457 753 5024
Fragments arranged by size:
5024 1457 753 171
EcoO109I rG'GnC_Cy
Cuts at: 845 3960 4542 845
Size: 3115 582 3708
Fragments arranged by size:
3708 3115 582
EcoRI G'AATT_C
Cuts at: 219 4407 219
Size: 4188 3217
EcoRV GAT'ATC
Cuts at: 1032 2654 4236 1032
Size: 1622 1582 4201
Fragments arranged by size:
4201 1622 1582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 566 683 5422 566
Size: 117 4739 2549
Fragments arranged by size:
4739 2549 117
FspI TGC'GCA
Cuts at: 5184 5184
Size: 7405
HaeII r_GCGC'y
Cuts at: 3170 4837 5085 6109 7088 3170
Size: 1667 248 1024 979 3487
Fragments arranged by size:
3487 1667 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 538 559 570 591 1554 1586 2966
Size: 32 343 21 11 21 963 32 1380
Cuts at: 2966 2998 3853 3885 3901 3933 4260 4292 5245
Size: 32 855 32 16 32 327 32 953
Cuts at: 5245 5275 5277 5307 163
Size: 30 2 30 2261
Fragments arranged by size:
2261 1380 963 953 855 343 327 32
32 32 32 32 32 30 30 21
21 16 11 2
HincII GTy'rAC
Cuts at: 815 4442 815
Size: 3627 3778
HindIII A'AGCT_T
Cuts at: 1217 1217
Size: 7405
KasI G'GCGC_C
Cuts at: 4833 5081 4833
Size: 248 7157
KpnI G_GTAC'C
Cuts at: 2086 4423 2086
Size: 2337 5068
MfeI C'AATT_G
Cuts at: 212 1823 2225 212
Size: 1611 402 5392
Fragments arranged by size:
5392 1611 402
MluI A'CGCG_T
Cuts at: 6838 6838
Size: 7405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4239 6076 6260 4239
Size: 1837 184 5384
Fragments arranged by size:
5384 1837 184
MscI TGG'CCA
Cuts at: 2141 2368 2720 5164 2141
Size: 227 352 2444 4382
Fragments arranged by size:
4382 2444 352 227
MslI CAynn'nnrTG
Cuts at: 1354 1492 1888 5392 5519 1354
Size: 138 396 3504 127 3240
Fragments arranged by size:
3504 3240 396 138 127
MspA1I CmG'CkG
Cuts at: 702 2299 2512 2576 3439 3641 3703 3818 4224
Size: 1597 213 64 863 202 62 115 406
Cuts at: 4224 4514 4828 5188 6203 6448 702
Size: 290 314 360 1015 245 1659
Fragments arranged by size:
1659 1597 1015 863 406 360 314 290
245 213 202 115 64 62
NaeI GCC'GGC
Cuts at: 1599 5584 1599
Size: 3985 3420
NarI GG'CG_CC
Cuts at: 4834 5082 4834
Size: 248 7157
NcoI C'CATG_G
Cuts at: 1389 2868 1389
Size: 1479 5926
NgoMIV G'CCGG_C
Cuts at: 1597 5582 1597
Size: 3985 3420
NheI G'CTAG_C
Cuts at: 1285 1285
Size: 7405
NruI TCG'CGA
Cuts at: 5766 5766
Size: 7405
NspI r_CATG'y
Cuts at: 431 4456 4956 5865 431
Size: 4025 500 909 1971
Fragments arranged by size:
4025 1971 909 500
PasI CC'CwG_GG
Cuts at: 2322 2700 4102 2322
Size: 378 1402 5625
Fragments arranged by size:
5625 1402 378
PciI A'CATG_T
Cuts at: 427 5861 427
Size: 5434 1971
PflMI CCAn_nnn'nTGG
Cuts at: 2148 2148
Size: 7405
PfoI T'CCnGG_A
Cuts at: 2028 3749 2028
Size: 1721 5684
PmeI GTTT'AAAC
Cuts at: 4402 4402
Size: 7405
PmlI CAC'GTG
Cuts at: 1581 1926 1581
Size: 345 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 980 1012 6544 6576 980
Size: 32 5532 32 1809
Fragments arranged by size:
5532 1809 32 32
PpuMI rG'GwC_Cy
Cuts at: 845 3960 845
Size: 3115 4290
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2616 2648 2616
Size: 32 7373
PstI C_TGCA'G
Cuts at: 396 3594 4154 4450 5135 396
Size: 3198 560 296 685 2666
Fragments arranged by size:
3198 2666 685 560 296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7405
PvuII CAG'CTG
Cuts at: 2512 3439 3641 4224 4828 5188 2512
Size: 927 202 583 604 360 4729
Fragments arranged by size:
4729 927 604 583 360 202
RsrII CG'GwC_CG
Cuts at: 5598 5598
Size: 7405
SacI G_AGCT'C
Cuts at: 4417 4417
Size: 7405
SalI G'TCGA_C
Cuts at: 4440 4440
Size: 7405
SanDI GG'GwC_CC
Cuts at: 845 845
Size: 7405
SapI GCTCTTCn'nnn_
Cuts at: 2172 5426 5636 2172
Size: 3254 210 3941
Fragments arranged by size:
3941 3254 210
SbfI CC_TGCA'GG
Cuts at: 4450 4450
Size: 7405
ScaI AGT'ACT
Cuts at: 2634 3670 6922 2634
Size: 1036 3252 3117
Fragments arranged by size:
3252 3117 1036
SexAI A'CCwGG_T
Cuts at: 1908 1908
Size: 7405
SfcI C'TryA_G
Cuts at: 33 392 3395 3590 4150 4214 4446 5131 6126
Size: 359 3003 195 560 64 232 685 995
Cuts at: 6126 6317 33
Size: 191 1121
Fragments arranged by size:
3003 1121 995 685 560 359 232 195
191 64
SfoI GGC'GCC
Cuts at: 4835 5083 4835
Size: 248 7157
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7405
SmaI CCC'GGG
Cuts at: 4425 4425
Size: 7405
SmlI C'TyrA_G
Cuts at: 227 2459 2686 4558 5473 5967 6229 227
Size: 2232 227 1872 915 494 262 1403
Fragments arranged by size:
2232 1872 1403 915 494 262 227
SnaBI TAC'GTA
Cuts at: 4395 4395
Size: 7405
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7405
SphI G_CATG'C
Cuts at: 4456 4956 4456
Size: 500 6905
SspI AAT'ATT
Cuts at: 4314 4376 4314
Size: 62 7343
StyI C'CwwG_G
Cuts at: 1389 1719 2069 2156 2208 2279 2772 2868 4537
Size: 330 350 87 52 71 493 96 1669
Cuts at: 4537 4891 1389
Size: 354 3903
Fragments arranged by size:
3903 1669 493 354 350 330 96 87
71 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1362 3492 4986 5119 5578 5789 1362
Size: 2130 1494 133 459 211 2978
Fragments arranged by size:
2978 2130 1494 459 211 133
TatI w'GTAC_w
Cuts at: 1672 2632 3668 6920 1672
Size: 960 1036 3252 2157
Fragments arranged by size:
3252 2157 1036 960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6922 6954 6922
Size: 32 7373
Tth111I GACn'n_nGTC
Cuts at: 5200 5200
Size: 7405
XbaI T'CTAG_A
Cuts at: 670 4434 670
Size: 3764 3641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2763 4898 2763
Size: 2135 5270
XhoI C'TCGA_G
Cuts at: 227 227
Size: 7405
XmaI C'CCGG_G
Cuts at: 4423 4423
Size: 7405
XmnI GAAnn'nnTTC
Cuts at: 1276 1728 1276
Size: 452 6953
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BfrBI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII FseI
FspAI HpaI NdeI NotI NsiI PacI PshAI PsiI
PspOMI PspXI SacII SfiI SgrAI SrfI StuI SwaI
ZraI