Restriction map for
pF1KSDA1200
(Circular) (Six-base) MAPSORT of: pF1KSDA1200.seq Check: 8421 from: 1 to: 7231
pF1KSDA1200 7231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 591 2500 591
Size: 1909 5322
AccI GT'mk_AC
Cuts at: 161 1517 1541 4267 161
Size: 1356 24 2726 3125
Fragments arranged by size:
3125 2726 1356 24
Acc65I G'GTAC_C
Cuts at: 4245 4245
Size: 7231
AclI AA'CG_TT
Cuts at: 3949 4210 7071 3949
Size: 261 2861 4109
Fragments arranged by size:
4109 2861 261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 686 778 836 933 1573 2158 3099 3923
Size: 313 92 58 97 640 585 941 824
Cuts at: 3923 5054 5486 6235 6503 373
Size: 1131 432 749 268 1101
Fragments arranged by size:
1131 1101 941 824 749 640 585 432
313 268 97 92 58
AflIII A'CryG_T
Cuts at: 3381 4581 5687 6664 3381
Size: 1200 1106 977 3948
Fragments arranged by size:
3948 1200 1106 977
AgeI A'CCGG_T
Cuts at: 5619 5619
Size: 7231
AleI CACnn'nnGTG
Cuts at: 2416 2416
Size: 7231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3142 3142
Size: 7231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 7199
AlwNI CAG_nnn'CTG
Cuts at: 261 273 570 591 756 1795 2133 2506 3201
Size: 12 297 21 165 1039 338 373 695
Cuts at: 3201 3592 4534 6103 261
Size: 391 942 1569 1389
Fragments arranged by size:
1569 1389 1039 942 695 391 373 338
297 165 21 12
ApaI G_GGCC'C
Cuts at: 3678 3678
Size: 7231
ApaLI G'TGCA_C
Cuts at: 3127 3916 6001 3127
Size: 789 2085 4357
Fragments arranged by size:
4357 2085 789
ApoI r'AATT_y
Cuts at: 4233 6584 6840 4233
Size: 2351 256 4624
Fragments arranged by size:
4624 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7231
AvaI C'yCGr_G
Cuts at: 1223 1235 1694 1962 2797 3267 4249 1223
Size: 12 459 268 835 470 982 4205
Fragments arranged by size:
4205 982 835 470 459 268 12
BamHI G'GATC_C
Cuts at: 607 2114 4254 607
Size: 1507 2140 3584
Fragments arranged by size:
3584 2140 1507
BanI G'GyrC_C
Cuts at: 147 663 787 1081 1115 2179 2461 2603 3695
Size: 516 124 294 34 1064 282 142 1092
Cuts at: 3695 4245 4659 4907 4942 147
Size: 550 414 248 35 2436
Fragments arranged by size:
2436 1092 1064 550 516 414 294 282
248 142 124 35 34
BanII G_rGCy'C
Cuts at: 431 916 1077 1306 1735 3678 4009 4243 5273
Size: 485 161 229 429 1943 331 234 1030
Cuts at: 5273 431
Size: 2389
Fragments arranged by size:
2389 1943 1030 485 429 331 234 229
161
BbeI G_GCGC'C
Cuts at: 151 4663 4911 151
Size: 4512 248 2471
Fragments arranged by size:
4512 2471 248
BbsI GAAGACnn'nnnn_
Cuts at: 1678 1909 3830 1678
Size: 231 1921 5079
Fragments arranged by size:
5079 1921 231
BbvCI CC'TCA_GC
Cuts at: 2918 3691 2918
Size: 773 6458
BciVI GTATCCnnnnn_n'
Cuts at: 2472 5127 5890 7231 2472
Size: 2655 763 1341 2472
Fragments arranged by size:
2655 2472 1341 763
BclI T'GATC_A
Cuts at: 4749 4749
Size: 7231
BfrBI ATG'CAT
Cuts at: 932 932
Size: 7231
BglI GCCn_nnn'nGGC
Cuts at: 3544 3596 3544
Size: 52 7179
BglII A'GATC_T
Cuts at: 2813 3308 3988 4744 6783 2813
Size: 495 680 756 2039 3261
Fragments arranged by size:
3261 2039 756 680 495
BlpI GC'TnA_GC
Cuts at: 449 1322 3617 3821 4341 449
Size: 873 2295 204 520 3339
Fragments arranged by size:
3339 2295 873 520 204
Bme1580I G_kGCm'C
Cuts at: 668 792 1148 2536 3131 3678 3920 4854 4947
Size: 124 356 1388 595 547 242 934 93
Cuts at: 4947 6005 668
Size: 1058 1894
Fragments arranged by size:
1894 1388 1058 934 595 547 356 242
124 93
BmgBI CAC'GTC
Cuts at: 1907 1907
Size: 7231
BmrI ACTGGGnnnn_n'
Cuts at: 4441 4522 4857 7003 4441
Size: 81 335 2146 4669
Fragments arranged by size:
4669 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1021 1200 5596 1021
Size: 179 4396 2656
Fragments arranged by size:
4396 2656 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 298 475 1237 1348 1882 2110 2125 2144
Size: 15 177 762 111 534 228 15 19
Cuts at: 2144 2399 2619 3178 3301 3613 3614 283
Size: 255 220 559 123 312 1 3900
Fragments arranged by size:
3900 762 559 534 312 255 228 220
177 123 111 19 15 15 1
Bpu10I CC'TnA_GC
Cuts at: 1211 1796 2918 3691 1211
Size: 585 1122 773 4751
Fragments arranged by size:
4751 1122 773 585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 520 571 1970 2671 3547 4405 5284 5778
Size: 399 51 1399 701 876 858 879 494
Cuts at: 5778 6076 121
Size: 298 1276
Fragments arranged by size:
1399 1276 879 876 858 701 494 399
298 51
BsaI GGTCTCn'nnnn_
Cuts at: 301 6935 301
Size: 6634 597
BsaAI yAC'GTr
Cuts at: 2483 4221 4582 2483
Size: 1738 361 5132
Fragments arranged by size:
5132 1738 361
BsaBI GATnn'nnATC
Cuts at: 6 4743 6782 6
Size: 4737 2039 455
Fragments arranged by size:
4737 2039 455
BsaHI Gr'CG_yC
Cuts at: 148 1712 1763 3884 4660 4908 5612 7108 148
Size: 1564 51 2121 776 248 704 1496 271
Fragments arranged by size:
2121 1564 1496 776 704 271 248 51
BsaWI w'CCGG_w
Cuts at: 203 383 1976 4040 4418 4939 5619 5893 6040
Size: 180 1593 2064 378 521 680 274 147
Cuts at: 6040 6451 203
Size: 411 983
Fragments arranged by size:
2064 1593 983 680 521 411 378 274
180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 724 754 2142 2172 3443 3473 724
Size: 30 1388 30 1271 30 4482
Fragments arranged by size:
4482 1388 1271 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1279 1647 2122 2443 2878 1279
Size: 368 475 321 435 5632
Fragments arranged by size:
5632 475 435 368 321
BseYI C'CCAG_C
Cuts at: 1275 1341 1839 2095 3830 4654 5412 5991 6487
Size: 66 498 256 1735 824 758 579 496
Cuts at: 6487 1275
Size: 2019
Fragments arranged by size:
2019 1735 824 758 579 498 496 256
66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1354 2164 2394 3337 364
Size: 990 810 230 943 4258
Fragments arranged by size:
4258 990 943 810 230
BsiEI CG_ry'CG
Cuts at: 104 2087 4771 4817 6027 104
Size: 1983 2684 46 1210 1308
Fragments arranged by size:
2684 1983 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 431 1735 1802 2438 2904 3131 3287 3920 4009
Size: 1304 67 636 466 227 156 633 89
Cuts at: 4009 4243 5021 5211 6005 431
Size: 234 778 190 794 1657
Fragments arranged by size:
1657 1304 794 778 636 633 466 234
227 190 156 89 67
BsmBI CGTCTCn'nnnn_
Cuts at: 1719 3433 1719
Size: 1714 5517
Bsp1286I G_dGCh'C
Cuts at: 431 668 792 916 1077 1148 1306 1735 1802
Size: 237 124 124 161 71 158 429 67
Cuts at: 1802 2438 2536 2904 3131 3287 3678 3920 4009
Size: 636 98 368 227 156 391 242 89
Cuts at: 4009 4243 4854 4947 5021 5211 5273 6005 431
Size: 234 611 93 74 190 62 732 1657
Fragments arranged by size:
1657 732 636 611 429 391 368 242
237 234 227 190 161 158 156 124
124 98 93 89 74 71 67 62
BspEI T'CCGG_A
Cuts at: 1976 4040 6451 1976
Size: 2064 2411 2756
Fragments arranged by size:
2756 2411 2064
BspHI T'CATG_A
Cuts at: 2828 6407 2828
Size: 3579 3652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1886 2500 2769 3624 4279 4493 4795 5176
Size: 1295 614 269 855 655 214 302 381
Cuts at: 5176 591
Size: 2646
Fragments arranged by size:
2646 1295 855 655 614 381 302 269
214
BsrBI CCG'CTC
Cuts at: 50 2595 3021 3042 5521 5575 7061 7226 50
Size: 2545 426 21 2479 54 1486 165 55
Fragments arranged by size:
2545 2479 1486 426 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 3730 4098 5141 3730
Size: 368 1043 5820
Fragments arranged by size:
5820 1043 368
BsrFI r'CCGG_y
Cuts at: 150 2724 5227 5408 5619 150
Size: 2574 2503 181 211 1762
Fragments arranged by size:
2574 2503 1762 211 181
BsrGI T'GTAC_A
Cuts at: 3398 3398
Size: 7231
BssSI C'ACGA_G
Cuts at: 5500 5860 5500
Size: 360 6871
BstAPI GCAn_nnn'nTGC
Cuts at: 273 1806 2610 3291 4606 273
Size: 1533 804 681 1315 2898
Fragments arranged by size:
2898 1533 1315 804 681
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7231
BstXI CCAn_nnnn'nTGG
Cuts at: 736 1201 2103 3157 736
Size: 465 902 1054 4810
Fragments arranged by size:
4810 1054 902 465
BstYI r'GATC_y
Cuts at: 1 607 1397 1998 2114 2580 2813 2967 3308
Size: 606 790 601 116 466 233 154 341
Cuts at: 3308 3988 4254 4721 4744 5079 5325 6339 6425
Size: 680 266 467 23 335 246 1014 86
Cuts at: 6425 6437 6783 1
Size: 12 346 449
Fragments arranged by size:
1014 790 680 606 601 467 466 449
346 341 335 266 246 233 154 116
86 23 12
BtgI C'CryG_G
Cuts at: 125 1087 1140 3176 3776 125
Size: 962 53 2036 600 3580
Fragments arranged by size:
3580 2036 962 600 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3041 5179 5360 5523 3041
Size: 2138 181 163 4749
Fragments arranged by size:
4749 2138 181 163
BtsI GCAGTG_nn'
Cuts at: 2406 2526 2534 2773 2881 3020 3285 4472 2406
Size: 120 8 239 108 139 265 1187 5165
Fragments arranged by size:
5165 1187 265 239 139 120 108 8
ClaI AT'CG_AT
Cuts at: 2346 2346
Size: 7231
DraI TTT'AAA
Cuts at: 4228 4228
Size: 7231
DrdI GACnn_nn'nnGTC
Cuts at: 4935 5795 4935
Size: 860 6371
EaeI y'GGCC_r
Cuts at: 152 1106 1152 1409 2084 2223 2985 3779 4082
Size: 954 46 257 675 139 762 794 303
Cuts at: 4082 4814 4988 5379 5406 6753 152
Size: 732 174 391 27 1347 630
Fragments arranged by size:
1347 954 794 762 732 675 630 391
303 257 174 139 46 27
EagI C'GGCC_G
Cuts at: 2084 4814 2084
Size: 2730 4501
EarI CTCTTCn'nnn_
Cuts at: 323 900 1906 2698 2716 3177 3256 3942 5252
Size: 577 1006 792 18 461 79 686 1310
Cuts at: 5252 5462 323
Size: 210 2092
Fragments arranged by size:
2092 1310 1006 792 686 577 461 210
79 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 5437 5749 5895 7041 199
Size: 5238 312 146 1146 389
Fragments arranged by size:
5238 1146 389 312 146
EcoICRI GAG'CTC
Cuts at: 429 1733 4007 4241 429
Size: 1304 2274 234 3419
Fragments arranged by size:
3419 2274 1304 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 298 373 475 686 778 836 933 1237
Size: 15 75 102 211 92 58 97 304
Cuts at: 1237 1348 1573 1882 2110 2125 2144 2158 2399
Size: 111 225 309 228 15 19 14 241
Cuts at: 2399 2619 3099 3178 3301 3613 3614 3923 5054
Size: 220 480 79 123 312 1 309 1131
Cuts at: 5054 5486 6235 6503 283
Size: 432 749 268 1011
Fragments arranged by size:
1131 1011 749 480 432 312 309 309
304 268 241 228 225 220 211 123
111 102 97 92 79 75 58 19
15 15 14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 782 1026 1063 1933 2104 2137 3270 782
Size: 244 37 870 171 33 1133 4743
Fragments arranged by size:
4743 1133 870 244 171 37 33
EcoO109I rG'GnC_Cy
Cuts at: 1232 1311 2682 3276 3589 3636 3674 3675 4368
Size: 79 1371 594 313 47 38 1 693
Cuts at: 4368 1232
Size: 4095
Fragments arranged by size:
4095 1371 693 594 313 79 47 38
1
EcoRI G'AATT_C
Cuts at: 4233 4233
Size: 7231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4053 5248 4053
Size: 1195 6036
FspI TGC'GCA
Cuts at: 2327 5010 2327
Size: 2683 4548
FspAI rTGC'GCAy
Cuts at: 2327 2327
Size: 7231
HaeII r_GCGC'y
Cuts at: 151 591 1920 3663 4663 4911 5935 6914 151
Size: 440 1329 1743 1000 248 1024 979 468
Fragments arranged by size:
1743 1329 1024 1000 979 468 440 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 724 756 1204 1236 1585 1609 1617
Size: 32 146 32 448 32 349 24 8
Cuts at: 1617 1641 1753 1785 1990 2022 3250 3282 3307
Size: 24 112 32 205 32 1228 32 25
Cuts at: 3307 3339 3991 4023 4035 4067 5071 5101 5103
Size: 32 652 32 12 32 1004 30 2
Cuts at: 5103 5133 546
Size: 30 2644
Fragments arranged by size:
2644 1228 1004 652 448 349 205 146
112 32 32 32 32 32 32 32
32 32 30 30 25 24 24 12
8 2
HincII GTy'rAC
Cuts at: 3713 4268 3713
Size: 555 6676
HindIII A'AGCT_T
Cuts at: 547 937 547
Size: 390 6841
KasI G'GCGC_C
Cuts at: 147 4659 4907 147
Size: 4512 248 2471
Fragments arranged by size:
4512 2471 248
KpnI G_GTAC'C
Cuts at: 4249 4249
Size: 7231
MluI A'CGCG_T
Cuts at: 3381 6664 3381
Size: 3283 3948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2979 5902 6086 2979
Size: 2923 184 4124
Fragments arranged by size:
4124 2923 184
MscI TGG'CCA
Cuts at: 1108 1411 2225 2987 3781 4084 4990 1108
Size: 303 814 762 794 303 906 3349
Fragments arranged by size:
3349 906 814 794 762 303 303
MslI CAynn'nnrTG
Cuts at: 2416 2719 3067 3124 3979 5218 5345 2416
Size: 303 348 57 855 1239 127 4302
Fragments arranged by size:
4302 1239 855 348 303 127 57
MspA1I CmG'CkG
Cuts at: 453 766 1089 1368 1679 2012 2843 3009 3198
Size: 313 323 279 311 333 831 166 189
Cuts at: 3198 3682 3690 3834 4149 4340 4654 5014 6029
Size: 484 8 144 315 191 314 360 1015
Cuts at: 6029 6274 453
Size: 245 1410
Fragments arranged by size:
1410 1015 831 484 360 333 323 315
314 313 311 279 245 191 189 166
144 8
NaeI GCC'GGC
Cuts at: 152 5410 152
Size: 5258 1973
NarI GG'CG_CC
Cuts at: 148 4660 4908 148
Size: 4512 248 2471
Fragments arranged by size:
4512 2471 248
NcoI C'CATG_G
Cuts at: 125 1140 125
Size: 1015 6216
NgoMIV G'CCGG_C
Cuts at: 150 5408 150
Size: 5258 1973
NruI TCG'CGA
Cuts at: 5592 5592
Size: 7231
NsiI A_TGCA'T
Cuts at: 934 934
Size: 7231
NspI r_CATG'y
Cuts at: 1425 1463 1584 4282 4782 5691 1425
Size: 38 121 2698 500 909 2965
Fragments arranged by size:
2965 2698 909 500 121 38
PasI CC'CwG_GG
Cuts at: 750 1009 1495 2301 750
Size: 259 486 806 5680
Fragments arranged by size:
5680 806 486 259
PciI A'CATG_T
Cuts at: 5687 5687
Size: 7231
PflMI CCAn_nnn'nTGG
Cuts at: 723 3592 3722 3788 723
Size: 2869 130 66 4166
Fragments arranged by size:
4166 2869 130 66
PfoI T'CCnGG_A
Cuts at: 1666 3893 1666
Size: 2227 5004
PmeI GTTT'AAAC
Cuts at: 4228 4228
Size: 7231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 6370 6402 3443
Size: 32 2895 32 4272
Fragments arranged by size:
4272 2895 32 32
PpuMI rG'GwC_Cy
Cuts at: 1232 3276 3636 1232
Size: 2044 360 4827
Fragments arranged by size:
4827 2044 360
PshAI GACnn'nnGTC
Cuts at: 3382 3382
Size: 7231
PspOMI G'GGCC_C
Cuts at: 3674 3674
Size: 7231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3397 3429 3397
Size: 32 7199
PstI C_TGCA'G
Cuts at: 684 797 1624 1811 2511 2780 2807 3296 3964
Size: 113 827 187 700 269 27 489 668
Cuts at: 3964 4276 4961 684
Size: 312 685 2954
Fragments arranged by size:
2954 827 700 685 668 489 312 269
187 113 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7231
PvuII CAG'CTG
Cuts at: 453 766 2843 3690 3834 4149 4654 5014 453
Size: 313 2077 847 144 315 505 360 2670
Fragments arranged by size:
2670 2077 847 505 360 315 313 144
RsrII CG'GwC_CG
Cuts at: 3032 3444 5424 3032
Size: 412 1980 4839
Fragments arranged by size:
4839 1980 412
SacI G_AGCT'C
Cuts at: 431 1735 4009 4243 431
Size: 1304 2274 234 3419
Fragments arranged by size:
3419 2274 1304 234
SacII CC_GC'GG
Cuts at: 1090 1090
Size: 7231
SalI G'TCGA_C
Cuts at: 4266 4266
Size: 7231
SapI GCTCTTCn'nnn_
Cuts at: 2698 2716 3177 5252 5462 2698
Size: 18 461 2075 210 4467
Fragments arranged by size:
4467 2075 461 210 18
SbfI CC_TGCA'GG
Cuts at: 3296 4276 3296
Size: 980 6251
ScaI AGT'ACT
Cuts at: 2123 2863 6748 2123
Size: 740 3885 2606
Fragments arranged by size:
3885 2606 740
SfcI C'TryA_G
Cuts at: 33 214 680 793 1620 1807 2507 2619 2776
Size: 181 466 113 827 187 700 112 157
Cuts at: 2776 2803 3292 3663 3960 4272 4957 5952 6143
Size: 27 489 371 297 312 685 995 191
Cuts at: 6143 33
Size: 1121
Fragments arranged by size:
1121 995 827 700 685 489 466 371
312 297 191 187 181 157 113 112
27
SfoI GGC'GCC
Cuts at: 149 4661 4909 149
Size: 4512 248 2471
Fragments arranged by size:
4512 2471 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7231
SmaI CCC'GGG
Cuts at: 1237 1696 4251 1237
Size: 459 2555 4217
Fragments arranged by size:
4217 2555 459
SmlI C'TyrA_G
Cuts at: 136 535 550 1985 2686 3526 4384 5299 5793
Size: 399 15 1435 701 840 858 915 494
Cuts at: 5793 6055 136
Size: 262 1312
Fragments arranged by size:
1435 1312 915 858 840 701 494 399
262 15
SnaBI TAC'GTA
Cuts at: 4221 4221
Size: 7231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7231
SphI G_CATG'C
Cuts at: 1425 1463 4282 4782 1425
Size: 38 2819 500 3874
Fragments arranged by size:
3874 2819 500 38
StuI AGG'CCT
Cuts at: 1180 2668 1180
Size: 1488 5743
StyI C'CwwG_G
Cuts at: 125 830 1140 1314 2210 2231 2525 3611 3842
Size: 705 310 174 896 21 294 1086 231
Cuts at: 3842 4199 4363 4717 125
Size: 357 164 354 2639
Fragments arranged by size:
2639 1086 896 705 357 354 310 294
231 174 164 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 516 1638 2109 3461 4812 4945 5404 5615
Size: 209 1122 471 1352 1351 133 459 211
Cuts at: 5615 307
Size: 1923
Fragments arranged by size:
1923 1352 1351 1122 471 459 211 209
133
TatI w'GTAC_w
Cuts at: 1949 2121 2861 3398 3413 6746 1949
Size: 172 740 537 15 3333 2434
Fragments arranged by size:
3333 2434 740 537 172 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2142 2174 3246 3278 6748 6780 2142
Size: 32 1072 32 3470 32 2593
Fragments arranged by size:
3470 2593 1072 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5026 5026
Size: 7231
XbaI T'CTAG_A
Cuts at: 4260 4260
Size: 7231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1138 1147 1836 3556 4077 4724 1138
Size: 9 689 1720 521 647 3645
Fragments arranged by size:
3645 1720 689 647 521 9
XmaI C'CCGG_G
Cuts at: 1235 1694 4249 1235
Size: 459 2555 4217
Fragments arranged by size:
4217 2555 459
XmnI GAAnn'nnTTC
Cuts at: 632 2263 632
Size: 1631 5600
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BcgI
BmtI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I CspCI
DraIII EcoRV FseI HpaI MfeI NdeI NheI NotI
PacI PmlI PsiI PspXI SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI