Restriction map for
pF1KSDA1201
(Circular) (Six-base) MAPSORT of: pF1KSDA1201.seq Check: 6908 from: 1 to: 5233
pF1KSDA1201 5233 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1724 1862 1724
Size: 138 5095
AccI GT'mk_AC
Cuts at: 2269 2269
Size: 5233
Acc65I G'GTAC_C
Cuts at: 2247 2247
Size: 5233
AclI AA'CG_TT
Cuts at: 1546 5073 1546
Size: 3527 1706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 364 1137 2140 3056 3488 4237 4505 282
Size: 82 773 1003 916 432 749 268 1010
Fragments arranged by size:
1010 1003 916 773 749 432 268 82
AflIII A'CryG_T
Cuts at: 1794 2183 2583 3689 4666 1794
Size: 389 400 1106 977 2361
Fragments arranged by size:
2361 1106 977 400 389
AgeI A'CCGG_T
Cuts at: 3621 3621
Size: 5233
AhdI GACnn_n'nnGTC
Cuts at: 1495 2184 1495
Size: 689 4544
AleI CACnn'nnGTG
Cuts at: 1468 1468
Size: 5233
AlwNI CAG_nnn'CTG
Cuts at: 2536 4105 2536
Size: 1569 3664
ApaI G_GGCC'C
Cuts at: 570 2043 570
Size: 1473 3760
ApaLI G'TGCA_C
Cuts at: 356 4003 356
Size: 3647 1586
ApoI r'AATT_y
Cuts at: 231 2235 4586 4842 231
Size: 2004 2351 256 622
Fragments arranged by size:
2351 2004 622 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5233
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5233
AvaI C'yCGr_G
Cuts at: 569 2251 569
Size: 1682 3551
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2050 2083 2050
Size: 33 5200
BamHI G'GATC_C
Cuts at: 2256 2256
Size: 5233
BanI G'GyrC_C
Cuts at: 2247 2661 2909 2944 2247
Size: 414 248 35 4536
Fragments arranged by size:
4536 414 248 35
BanII G_rGCy'C
Cuts at: 142 570 647 962 1061 1750 2043 2084 2245
Size: 428 77 315 99 689 293 41 161
Cuts at: 2245 3275 142
Size: 1030 2100
Fragments arranged by size:
2100 1030 689 428 315 293 161 99
77 41
BbeI G_GCGC'C
Cuts at: 2665 2913 2665
Size: 248 4985
BbsI GAAGACnn'nnnn_
Cuts at: 299 1137 299
Size: 838 4395
BciVI GTATCCnnnnn_n'
Cuts at: 736 3129 3892 5233 736
Size: 2393 763 1341 736
Fragments arranged by size:
2393 1341 763 736
BclI T'GATC_A
Cuts at: 2751 2751
Size: 5233
BglI GCCn_nnn'nGGC
Cuts at: 221 221
Size: 5233
BglII A'GATC_T
Cuts at: 2746 4785 2746
Size: 2039 3194
BlpI GC'TnA_GC
Cuts at: 2343 2343
Size: 5233
Bme1580I G_kGCm'C
Cuts at: 360 570 716 1424 2043 2856 2949 4007 360
Size: 210 146 708 619 813 93 1058 1586
Fragments arranged by size:
1586 1058 813 708 619 210 146 93
BmgBI CAC'GTC
Cuts at: 1419 2186 1419
Size: 767 4466
BmrI ACTGGGnnnn_n'
Cuts at: 201 2016 2443 2524 2859 5005 201
Size: 1815 427 81 335 2146 429
Fragments arranged by size:
2146 1815 429 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 391 3598 156
Size: 235 3207 1791
Fragments arranged by size:
3207 1791 235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1587 1597 937
Size: 650 10 4573
Fragments arranged by size:
4573 650 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2407 3286 3780 4078 2407
Size: 879 494 298 3562
Fragments arranged by size:
3562 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 802 1503 4937 802
Size: 701 3434 1098
Fragments arranged by size:
3434 1098 701
BsaAI yAC'GTr
Cuts at: 711 1134 1389 2223 2584 711
Size: 423 255 834 361 3360
Fragments arranged by size:
3360 834 423 361 255
BsaBI GATnn'nnATC
Cuts at: 6 2745 4784 6
Size: 2739 2039 455
Fragments arranged by size:
2739 2039 455
BsaHI Gr'CG_yC
Cuts at: 2662 2910 3614 5110 2662
Size: 248 704 1496 2785
Fragments arranged by size:
2785 1496 704 248
BsaWI w'CCGG_w
Cuts at: 174 2420 2941 3621 3895 4042 4453 174
Size: 2246 521 680 274 147 411 954
Fragments arranged by size:
2246 954 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1686 1716 1686
Size: 30 5203
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 192 1168 1276 1707 141
Size: 51 976 108 431 3667
Fragments arranged by size:
3667 976 431 108 51
BseYI C'CCAG_C
Cuts at: 437 2042 2656 3414 3993 4489 437
Size: 1605 614 758 579 496 1181
Fragments arranged by size:
1605 1181 758 614 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 5233
BsiEI CG_ry'CG
Cuts at: 104 216 2773 2819 4029 104
Size: 112 2557 46 1210 1308
Fragments arranged by size:
2557 1308 1210 112 46
BsiHKAI G_wGCw'C
Cuts at: 360 647 962 1061 1627 2084 2245 3023 3213
Size: 287 315 99 566 457 161 778 190
Cuts at: 3213 4007 360
Size: 794 1586
Fragments arranged by size:
1586 794 778 566 457 315 287 190
161 99
BsmI GAATG_Cn'
Cuts at: 615 615
Size: 5233
BsmBI CGTCTCn'nnnn_
Cuts at: 900 932 900
Size: 32 5201
Bsp1286I G_dGCh'C
Cuts at: 142 360 570 647 716 962 1061 1424 1627
Size: 218 210 77 69 246 99 363 203
Cuts at: 1627 1750 2043 2084 2245 2856 2949 3023 3213
Size: 123 293 41 161 611 93 74 190
Cuts at: 3213 3275 4007 142
Size: 62 732 1368
Fragments arranged by size:
1368 732 611 363 293 246 218 210
203 190 161 123 99 93 77 74
69 62 41
BspEI T'CCGG_A
Cuts at: 4453 4453
Size: 5233
BspHI T'CATG_A
Cuts at: 4409 4409
Size: 5233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1724 1792 1862 2281 2495 2797 3178 1724
Size: 68 70 419 214 302 381 3779
Fragments arranged by size:
3779 419 381 302 214 70 68
BsrBI CCG'CTC
Cuts at: 50 221 3523 3577 5063 5228 50
Size: 171 3302 54 1486 165 55
Fragments arranged by size:
3302 1486 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3143 3143
Size: 5233
BsrFI r'CCGG_y
Cuts at: 1124 3229 3410 3621 1124
Size: 2105 181 211 2736
Fragments arranged by size:
2736 2105 211 181
BsrGI T'GTAC_A
Cuts at: 1358 1358
Size: 5233
BssSI C'ACGA_G
Cuts at: 3502 3862 3502
Size: 360 4873
BstAPI GCAn_nnn'nTGC
Cuts at: 186 2608 186
Size: 2422 2811
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5233
BstEII G'GTnAC_C
Cuts at: 1752 2015 1752
Size: 263 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 670 1317 670
Size: 647 4586
BstYI r'GATC_y
Cuts at: 1 410 755 2256 2723 2746 3081 3327 4341
Size: 409 345 1501 467 23 335 246 1014
Cuts at: 4341 4427 4439 4785 1
Size: 86 12 346 449
Fragments arranged by size:
1501 1014 467 449 409 346 345 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 1249 125
Size: 1124 4109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3181 3362 3525 3181
Size: 181 163 4889
Fragments arranged by size:
4889 181 163
BtsI GCAGTG_nn'
Cuts at: 888 1982 2474 888
Size: 1094 492 3647
Fragments arranged by size:
3647 1094 492
DraI TTT'AAA
Cuts at: 2230 2230
Size: 5233
DraIII CAC_nnn'GTG
Cuts at: 1419 1419
Size: 5233
DrdI GACnn_nn'nnGTC
Cuts at: 2937 3797 2937
Size: 860 4373
EaeI y'GGCC_r
Cuts at: 213 1122 1610 2816 2990 3381 3408 4755 213
Size: 909 488 1206 174 391 27 1347 691
Fragments arranged by size:
1347 1206 909 691 488 391 174 27
EagI C'GGCC_G
Cuts at: 213 2816 213
Size: 2603 2630
EarI CTCTTCn'nnn_
Cuts at: 746 761 917 1074 1186 1730 1945 3254 3464
Size: 15 156 157 112 544 215 1309 210
Cuts at: 3464 746
Size: 2515
Fragments arranged by size:
2515 1309 544 215 210 157 156 112
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1579 3439 3751 3897 5043 1579
Size: 1860 312 146 1146 1769
Fragments arranged by size:
1860 1769 1146 312 146
EcoICRI GAG'CTC
Cuts at: 645 960 1059 2082 2243 645
Size: 315 99 1023 161 3635
Fragments arranged by size:
3635 1023 315 161 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 364 937 1137 1587 1597 2140 3056 3488
Size: 82 573 200 450 10 543 916 432
Cuts at: 3488 4237 4505 282
Size: 749 268 1010
Fragments arranged by size:
1010 916 749 573 543 450 432 268
200 82 10
EcoNI CCTnn'n_nnAGG
Cuts at: 952 1735 952
Size: 783 4450
EcoO109I rG'GnC_Cy
Cuts at: 566 888 944 1113 2171 2370 566
Size: 322 56 169 1058 199 3429
Fragments arranged by size:
3429 1058 322 199 169 56
EcoRI G'AATT_C
Cuts at: 231 2235 231
Size: 2004 3229
EcoRV GAT'ATC
Cuts at: 1236 1236
Size: 5233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 567 3250 567
Size: 2683 2550
FspI TGC'GCA
Cuts at: 3012 3012
Size: 5233
HaeII r_GCGC'y
Cuts at: 336 931 1227 2665 2913 3937 4916 336
Size: 595 296 1438 248 1024 979 653
Fragments arranged by size:
1438 1024 979 653 595 296 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 380 412 775 807 987 1019 1481
Size: 32 212 32 363 32 180 32 462
Cuts at: 1481 1513 1686 1718 2170 2202 3073 3103 3105
Size: 32 173 32 452 32 871 30 2
Cuts at: 3105 3135 136
Size: 30 2234
Fragments arranged by size:
2234 871 462 452 363 212 180 173
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2270 2270
Size: 5233
KasI G'GCGC_C
Cuts at: 2661 2909 2661
Size: 248 4985
KpnI G_GTAC'C
Cuts at: 2251 2251
Size: 5233
MluI A'CGCG_T
Cuts at: 4666 4666
Size: 5233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1097 1940 2168 3904 4088 1097
Size: 843 228 1736 184 2242
Fragments arranged by size:
2242 1736 843 228 184
MscI TGG'CCA
Cuts at: 1612 2992 1612
Size: 1380 3853
MslI CAynn'nnrTG
Cuts at: 1468 3220 3347 1468
Size: 1752 127 3354
Fragments arranged by size:
3354 1752 127
MspA1I CmG'CkG
Cuts at: 197 437 834 1203 1221 1889 2342 2656 3016
Size: 240 397 369 18 668 453 314 360
Cuts at: 3016 4031 4276 197
Size: 1015 245 1154
Fragments arranged by size:
1154 1015 668 453 397 369 360 314
245 240 18
NaeI GCC'GGC
Cuts at: 1126 3412 1126
Size: 2286 2947
NarI GG'CG_CC
Cuts at: 2662 2910 2662
Size: 248 4985
NcoI C'CATG_G
Cuts at: 125 1249 125
Size: 1124 4109
NgoMIV G'CCGG_C
Cuts at: 1124 3410 1124
Size: 2286 2947
NruI TCG'CGA
Cuts at: 3594 3594
Size: 5233
NspI r_CATG'y
Cuts at: 1798 2284 2784 3693 1798
Size: 486 500 909 3338
Fragments arranged by size:
3338 909 500 486
PasI CC'CwG_GG
Cuts at: 1529 1529
Size: 5233
PciI A'CATG_T
Cuts at: 1794 3689 1794
Size: 1895 3338
PfoI T'CCnGG_A
Cuts at: 1826 1826
Size: 5233
PmeI GTTT'AAAC
Cuts at: 2230 2230
Size: 5233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4372 4404 4372
Size: 32 5201
PpuMI rG'GwC_Cy
Cuts at: 944 1113 2171 944
Size: 169 1058 4006
Fragments arranged by size:
4006 1058 169
PspOMI G'GGCC_C
Cuts at: 566 2039 566
Size: 1473 3760
PstI C_TGCA'G
Cuts at: 1859 2278 2963 1859
Size: 419 685 4129
Fragments arranged by size:
4129 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5233
PvuII CAG'CTG
Cuts at: 197 834 1889 2656 3016 197
Size: 637 1055 767 360 2414
Fragments arranged by size:
2414 1055 767 637 360
RsrII CG'GwC_CG
Cuts at: 3426 3426
Size: 5233
SacI G_AGCT'C
Cuts at: 647 962 1061 2084 2245 647
Size: 315 99 1023 161 3635
Fragments arranged by size:
3635 1023 315 161 99
SalI G'TCGA_C
Cuts at: 2268 2268
Size: 5233
SanDI GG'GwC_CC
Cuts at: 944 944
Size: 5233
SapI GCTCTTCn'nnn_
Cuts at: 1945 3254 3464 1945
Size: 1309 210 3714
Fragments arranged by size:
3714 1309 210
SbfI CC_TGCA'GG
Cuts at: 2278 2278
Size: 5233
ScaI AGT'ACT
Cuts at: 4750 4750
Size: 5233
SfcI C'TryA_G
Cuts at: 33 420 1855 2274 2959 3954 4145 33
Size: 387 1435 419 685 995 191 1121
Fragments arranged by size:
1435 1121 995 685 419 387 191
SfoI GGC'GCC
Cuts at: 2663 2911 2663
Size: 248 4985
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5233
SmaI CCC'GGG
Cuts at: 571 2253 571
Size: 1682 3551
SmlI C'TyrA_G
Cuts at: 2386 3301 3795 4057 2386
Size: 915 494 262 3562
Fragments arranged by size:
3562 915 494 262
SnaBI TAC'GTA
Cuts at: 2223 2223
Size: 5233
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5233
SphI G_CATG'C
Cuts at: 2284 2784 2284
Size: 500 4733
StuI AGG'CCT
Cuts at: 1102 1102
Size: 5233
StyI C'CwwG_G
Cuts at: 125 638 1249 2365 2719 125
Size: 513 611 1116 354 2639
Fragments arranged by size:
2639 1116 611 513 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1426 2814 2947 3406 3617 1426
Size: 1388 133 459 211 3042
Fragments arranged by size:
3042 1388 459 211 133
TatI w'GTAC_w
Cuts at: 1358 4748 1358
Size: 3390 1843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4750 4782 4750
Size: 32 5201
Tth111I GACn'n_nGTC
Cuts at: 3028 3028
Size: 5233
XbaI T'CTAG_A
Cuts at: 2262 2262
Size: 5233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1875 2726 1875
Size: 851 4382
XmaI C'CCGG_G
Cuts at: 569 2251 569
Size: 1682 3551
XmnI GAAnn'nnTTC
Cuts at: 1546 1561 1546
Size: 15 5218
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BssHII BstZ17I Bsu36I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI