Restriction map for
pF1KSDA1202
(Circular) (Six-base) MAPSORT of: pF1KSDA1202.seq Check: 5521 from: 1 to: 7633
pF1KSDA1202 7633 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1394 1771 1394
Size: 377 7256
AccI GT'mk_AC
Cuts at: 4596 4669 4596
Size: 73 7560
Acc65I G'GTAC_C
Cuts at: 4647 4647
Size: 7633
AclI AA'CG_TT
Cuts at: 7473 7473
Size: 7633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 480 509 958 2659 3221 3914 4432 5456
Size: 1 29 449 1701 562 693 518 1024
Cuts at: 5456 5888 6637 6905 479
Size: 432 749 268 1207
Fragments arranged by size:
1701 1207 1024 749 693 562 518 449
432 268 29 1
AflII C'TTAA_G
Cuts at: 199 199
Size: 7633
AflIII A'CryG_T
Cuts at: 533 665 4983 6089 7066 533
Size: 132 4318 1106 977 1100
Fragments arranged by size:
4318 1106 1100 977 132
AgeI A'CCGG_T
Cuts at: 6021 6021
Size: 7633
AleI CACnn'nnGTG
Cuts at: 1962 2756 1962
Size: 794 6839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1724 1724
Size: 7633
AlwNI CAG_nnn'CTG
Cuts at: 1374 2260 2780 4380 4936 6505 1374
Size: 886 520 1600 556 1569 2502
Fragments arranged by size:
2502 1600 1569 886 556 520
ApaI G_GGCC'C
Cuts at: 923 943 3939 4610 923
Size: 20 2996 671 3946
Fragments arranged by size:
3946 2996 671 20
ApaLI G'TGCA_C
Cuts at: 1396 6403 1396
Size: 5007 2626
ApoI r'AATT_y
Cuts at: 1910 2727 3022 4222 4635 6986 7242 1910
Size: 817 295 1200 413 2351 256 2301
Fragments arranged by size:
2351 2301 1200 817 413 295 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7633
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7633
AvaI C'yCGr_G
Cuts at: 1847 2962 3335 3720 4301 4561 4651 1847
Size: 1115 373 385 581 260 90 4829
Fragments arranged by size:
4829 1115 581 385 373 260 90
AvrII C'CTAG_G
Cuts at: 4041 4041
Size: 7633
BamHI G'GATC_C
Cuts at: 1228 4656 1228
Size: 3428 4205
BanI G'GyrC_C
Cuts at: 180 1451 2591 2900 2979 3321 4647 5061 5309
Size: 1271 1140 309 79 342 1326 414 248
Cuts at: 5309 5344 180
Size: 35 2469
Fragments arranged by size:
2469 1326 1271 1140 414 342 309 248
79 35
BanII G_rGCy'C
Cuts at: 923 943 990 1189 1433 1805 2003 2572 2811
Size: 20 47 199 244 372 198 569 239
Cuts at: 2811 3259 3365 3721 3939 4610 4645 5675 923
Size: 448 106 356 218 671 35 1030 2881
Fragments arranged by size:
2881 1030 671 569 448 372 356 244
239 218 199 198 106 47 35 20
BbeI G_GCGC'C
Cuts at: 2595 3325 5065 5313 2595
Size: 730 1740 248 4915
Fragments arranged by size:
4915 1740 730 248
BbsI GAAGACnn'nnnn_
Cuts at: 2108 3070 2108
Size: 962 6671
BbvCI CC'TCA_GC
Cuts at: 2128 2948 3697 2128
Size: 820 749 6064
Fragments arranged by size:
6064 820 749
BciVI GTATCCnnnnn_n'
Cuts at: 901 5529 6292 7633 901
Size: 4628 763 1341 901
Fragments arranged by size:
4628 1341 901 763
BclI T'GATC_A
Cuts at: 5151 5151
Size: 7633
BfrBI ATG'CAT
Cuts at: 477 2918 477
Size: 2441 5192
BglI GCCn_nnn'nGGC
Cuts at: 1184 1184
Size: 7633
BglII A'GATC_T
Cuts at: 5146 7185 5146
Size: 2039 5594
BlpI GC'TnA_GC
Cuts at: 3830 4743 3830
Size: 913 6720
Bme1580I G_kGCm'C
Cuts at: 183 735 923 943 1362 1400 1454 2961 3939
Size: 552 188 20 419 38 54 1507 978
Cuts at: 3939 4610 5256 5349 6407 183
Size: 671 646 93 1058 1409
Fragments arranged by size:
1507 1409 1058 978 671 646 552 419
188 93 54 38 20
BmgBI CAC'GTC
Cuts at: 534 534
Size: 7633
BmrI ACTGGGnnnn_n'
Cuts at: 800 3381 3459 3618 4843 4924 5259 7405 800
Size: 2581 78 159 1225 81 335 2146 1028
Fragments arranged by size:
2581 2146 1225 1028 335 159 81 78
BmtI G_CTAG'C
Cuts at: 1300 1300
Size: 7633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 1009 2098 2327 3273 5998 777
Size: 232 1089 229 946 2725 2412
Fragments arranged by size:
2725 2412 1089 946 232 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 631 938 966 1918 2297 2556 3733 4564
Size: 165 307 28 952 379 259 1177 831
Cuts at: 4564 466
Size: 3535
Fragments arranged by size:
3535 1177 952 831 379 307 259 165
28
Bpu10I CC'TnA_GC
Cuts at: 1301 2128 2948 3697 3821 1301
Size: 827 820 749 124 5113
Fragments arranged by size:
5113 827 820 749 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 2387 3160 4807 5686 6180 6478 361
Size: 2026 773 1647 879 494 298 1516
Fragments arranged by size:
2026 1647 1516 879 773 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 823 1984 2315 2526 3376 4461 7337 823
Size: 1161 331 211 850 1085 2876 1119
Fragments arranged by size:
2876 1161 1119 1085 850 331 211
BsaAI yAC'GTr
Cuts at: 4422 4623 4984 4422
Size: 201 361 7071
Fragments arranged by size:
7071 361 201
BsaBI GATnn'nnATC
Cuts at: 6 5145 7184 6
Size: 5139 2039 455
Fragments arranged by size:
5139 2039 455
BsaHI Gr'CG_yC
Cuts at: 2592 3322 5062 5310 6014 7510 2592
Size: 730 1740 248 704 1496 2715
Fragments arranged by size:
2715 1740 1496 730 704 248
BsaWI w'CCGG_w
Cuts at: 368 4820 5341 6021 6295 6442 6853 368
Size: 4452 521 680 274 147 411 1148
Fragments arranged by size:
4452 1148 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2233 2263 2798 2828 2233
Size: 30 535 30 7038
Fragments arranged by size:
7038 535 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 766 985 1932 2241 3262 3416 3419 3428
Size: 358 219 947 309 1021 154 3 9
Cuts at: 3428 3431 3547 3550 3553 3556 3559 3562 3595
Size: 3 116 3 3 3 3 3 33
Cuts at: 3595 3598 3601 3604 3613 3616 3619 3622 3625
Size: 3 3 3 9 3 3 3 3
Cuts at: 3625 3685 4063 4195 4561 408
Size: 60 378 132 366 3480
Fragments arranged by size:
3480 1021 947 378 366 358 309 219
154 132 116 60 33 9 9 3
3 3 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 874 1928 2065 2407 3147 3479 3744 4133 4609
Size: 1054 137 342 740 332 265 389 476
Cuts at: 4609 5056 5814 6393 6889 874
Size: 447 758 579 496 1618
Fragments arranged by size:
1618 1054 758 740 579 496 476 447
389 342 332 265 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 562 1833 3037 3494 184
Size: 378 1271 1204 457 4323
Fragments arranged by size:
4323 1271 1204 457 378
BsiEI CG_ry'CG
Cuts at: 104 5173 5219 6429 104
Size: 5069 46 1210 1308
Fragments arranged by size:
5069 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1400 1433 2572 2811 3259 3365 3660 4421 4645
Size: 33 1139 239 448 106 295 761 224
Cuts at: 4645 5423 5613 6407 1400
Size: 778 190 794 2626
Fragments arranged by size:
2626 1139 794 778 761 448 295 239
224 190 106 33
BsmI GAATG_Cn'
Cuts at: 510 2918 4317 510
Size: 2408 1399 3826
Fragments arranged by size:
3826 2408 1399
Bsp1286I G_dGCh'C
Cuts at: 183 735 923 943 990 1189 1362 1400 1433
Size: 552 188 20 47 199 173 38 33
Cuts at: 1433 1454 1805 2003 2572 2811 2961 3259 3365
Size: 21 351 198 569 239 150 298 106
Cuts at: 3365 3660 3721 3939 4421 4610 4645 5256 5349
Size: 295 61 218 482 189 35 611 93
Cuts at: 5349 5423 5613 5675 6407 183
Size: 74 190 62 732 1409
Fragments arranged by size:
1409 732 611 569 552 482 351 298
295 239 218 199 198 190 189 188
173 150 106 93 74 62 61 47
38 35 33 21 20
BspEI T'CCGG_A
Cuts at: 368 6853 368
Size: 6485 1148
BspHI T'CATG_A
Cuts at: 6809 6809
Size: 7633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1394 1771 2788 4475 4681 4895 5197 5578 1394
Size: 377 1017 1687 206 214 302 381 3449
Fragments arranged by size:
3449 1687 1017 381 377 302 214 206
BsrBI CCG'CTC
Cuts at: 50 228 1856 5923 5977 7463 7628 50
Size: 178 1628 4067 54 1486 165 55
Fragments arranged by size:
4067 1628 1486 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 5543 564
Size: 4979 2654
BsrFI r'CCGG_y
Cuts at: 134 812 1868 2897 5629 5810 6021 134
Size: 678 1056 1029 2732 181 211 1746
Fragments arranged by size:
2732 1746 1056 1029 678 211 181
BssSI C'ACGA_G
Cuts at: 5902 6262 5902
Size: 360 7273
BstAPI GCAn_nnn'nTGC
Cuts at: 1395 5008 1395
Size: 3613 4020
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7633
BstXI CCAn_nnnn'nTGG
Cuts at: 444 1563 1689 2197 444
Size: 1119 126 508 5880
Fragments arranged by size:
5880 1119 508 126
BstYI r'GATC_y
Cuts at: 1 1228 3393 3811 4656 5123 5146 5481 5727
Size: 1227 2165 418 845 467 23 335 246
Cuts at: 5727 6741 6827 6839 7185 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2165 1227 1014 845 467 449 418 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 742 834 3668 4472 742
Size: 92 2834 804 3903
Fragments arranged by size:
3903 2834 804 92
BtgI C'CryG_G
Cuts at: 125 1736 2289 2348 2600 125
Size: 1611 553 59 252 5158
Fragments arranged by size:
5158 1611 553 252 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1333 5581 5762 5925 1333
Size: 4248 181 163 3041
Fragments arranged by size:
4248 3041 181 163
BtsI GCAGTG_nn'
Cuts at: 550 2021 2802 3280 4874 550
Size: 1471 781 478 1594 3309
Fragments arranged by size:
3309 1594 1471 781 478
ClaI AT'CG_AT
Cuts at: 4328 4328
Size: 7633
DraI TTT'AAA
Cuts at: 4630 4630
Size: 7633
DraIII CAC_nnn'GTG
Cuts at: 2228 3739 2228
Size: 1511 6122
DrdI GACnn_nn'nnGTC
Cuts at: 3072 4264 5337 6197 3072
Size: 1192 1073 860 4508
Fragments arranged by size:
4508 1192 1073 860
EaeI y'GGCC_r
Cuts at: 434 707 810 1691 2187 2199 3705 3983 5216
Size: 273 103 881 496 12 1506 278 1233
Cuts at: 5216 5390 5781 5808 7155 434
Size: 174 391 27 1347 912
Fragments arranged by size:
1506 1347 1233 912 881 496 391 278
273 174 103 27 12
EagI C'GGCC_G
Cuts at: 5216 5216
Size: 7633
EarI CTCTTCn'nnn_
Cuts at: 1870 1982 2045 2089 2453 3560 3566 3572 4562
Size: 112 63 44 364 1107 6 6 990
Cuts at: 4562 5654 5864 1870
Size: 1092 210 3639
Fragments arranged by size:
3639 1107 1092 990 364 210 112 63
44 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1887 3885 5839 6151 6297 7443 1887
Size: 1998 1954 312 146 1146 2077
Fragments arranged by size:
2077 1998 1954 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1431 2570 2809 3257 3363 4643 1431
Size: 1139 239 448 106 1280 4421
Fragments arranged by size:
4421 1280 1139 448 239 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 479 480 509 631 938 958 966 1918
Size: 13 1 29 122 307 20 8 952
Cuts at: 1918 2297 2556 2659 3221 3733 3914 4432 4564
Size: 379 259 103 562 512 181 518 132
Cuts at: 4564 5456 5888 6637 6905 466
Size: 892 432 749 268 1194
Fragments arranged by size:
1194 952 892 749 562 518 512 432
379 307 268 259 181 132 122 103
29 20 13 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1517 3929 4045 4177 4471 1517
Size: 2412 116 132 294 4679
Fragments arranged by size:
4679 2412 294 132 116
EcoO109I rG'GnC_Cy
Cuts at: 143 342 801 919 939 1336 1844 1948 2356
Size: 199 459 118 20 397 508 104 408
Cuts at: 2356 2777 3675 3935 3936 4251 4606 4770 143
Size: 421 898 260 1 315 355 164 3006
Fragments arranged by size:
3006 898 508 459 421 408 397 355
315 260 199 164 118 104 20 1
EcoRI G'AATT_C
Cuts at: 4635 4635
Size: 7633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1968 3123 3435 4254 5650 1968
Size: 1155 312 819 1396 3951
Fragments arranged by size:
3951 1396 1155 819 312
FseI GG_CCGG'CC
Cuts at: 816 816
Size: 7633
FspI TGC'GCA
Cuts at: 3286 5412 3286
Size: 2126 5507
HaeII r_GCGC'y
Cuts at: 2595 3325 5065 5313 6337 7316 2595
Size: 730 1740 248 1024 979 2912
Fragments arranged by size:
2912 1740 1024 979 730 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1590 2233 2265 2580 2612 2798 2830 3373
Size: 32 643 32 315 32 186 32 543
Cuts at: 3373 3405 3742 3774 5473 5503 5505 5535 1558
Size: 32 337 32 1699 30 2 30 3656
Fragments arranged by size:
3656 1699 643 543 337 315 186 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4059 4269 4670 4059
Size: 210 401 7022
Fragments arranged by size:
7022 401 210
KasI G'GCGC_C
Cuts at: 2591 3321 5061 5309 2591
Size: 730 1740 248 4915
Fragments arranged by size:
4915 1740 730 248
KpnI G_GTAC'C
Cuts at: 4651 4651
Size: 7633
MluI A'CGCG_T
Cuts at: 7066 7066
Size: 7633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1206 1292 2387 3371 6304 6488 1206
Size: 86 1095 984 2933 184 2351
Fragments arranged by size:
2933 2351 1095 984 184 86
MscI TGG'CCA
Cuts at: 436 1693 2189 2201 3707 3985 5392 436
Size: 1257 496 12 1506 278 1407 2677
Fragments arranged by size:
2677 1506 1407 1257 496 278 12
MslI CAynn'nnrTG
Cuts at: 472 1962 2756 3776 5620 5747 472
Size: 1490 794 1020 1844 127 2358
Fragments arranged by size:
2358 1844 1490 1020 794 127
MspA1I CmG'CkG
Cuts at: 168 1371 1962 2576 4380 4742 5056 5416 6431
Size: 1203 591 614 1804 362 314 360 1015
Cuts at: 6431 6676 168
Size: 245 1125
Fragments arranged by size:
1804 1203 1125 1015 614 591 362 360
314 245
NaeI GCC'GGC
Cuts at: 814 5812 814
Size: 4998 2635
NarI GG'CG_CC
Cuts at: 2592 3322 5062 5310 2592
Size: 730 1740 248 4915
Fragments arranged by size:
4915 1740 730 248
NcoI C'CATG_G
Cuts at: 125 2348 2600 125
Size: 2223 252 5158
Fragments arranged by size:
5158 2223 252
NgoMIV G'CCGG_C
Cuts at: 812 5810 812
Size: 4998 2635
NheI G'CTAG_C
Cuts at: 1296 1296
Size: 7633
NruI TCG'CGA
Cuts at: 5994 5994
Size: 7633
NsiI A_TGCA'T
Cuts at: 479 2920 479
Size: 2441 5192
NspI r_CATG'y
Cuts at: 433 669 1396 2275 3214 4684 5184 6093 433
Size: 236 727 879 939 1470 500 909 1973
Fragments arranged by size:
1973 1470 939 909 879 727 500 236
PasI CC'CwG_GG
Cuts at: 185 787 185
Size: 602 7031
PciI A'CATG_T
Cuts at: 665 6089 665
Size: 5424 2209
PflMI CCAn_nnn'nTGG
Cuts at: 325 2601 4068 325
Size: 2276 1467 3890
Fragments arranged by size:
3890 2276 1467
PfoI T'CCnGG_A
Cuts at: 1652 1652
Size: 7633
PmeI GTTT'AAAC
Cuts at: 4630 4630
Size: 7633
PmlI CAC'GTG
Cuts at: 4422 4422
Size: 7633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3714 3746 6772 6804 3714
Size: 32 3026 32 4543
Fragments arranged by size:
4543 3026 32 32
PpuMI rG'GwC_Cy
Cuts at: 143 1336 1844 1948 2777 3675 4251 143
Size: 1193 508 104 829 898 576 3525
Fragments arranged by size:
3525 1193 898 829 576 508 104
PspOMI G'GGCC_C
Cuts at: 919 939 3935 4606 919
Size: 20 2996 671 3946
Fragments arranged by size:
3946 2996 671 20
PspXI vC'TCGA_Gb
Cuts at: 2962 2962
Size: 7633
PstI C_TGCA'G
Cuts at: 1293 1391 2797 4678 5363 1293
Size: 98 1406 1881 685 3563
Fragments arranged by size:
3563 1881 1406 685 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7633
PvuII CAG'CTG
Cuts at: 168 1371 1962 2576 4380 5056 5416 168
Size: 1203 591 614 1804 676 360 2385
Fragments arranged by size:
2385 1804 1203 676 614 591 360
RsrII CG'GwC_CG
Cuts at: 5826 5826
Size: 7633
SacI G_AGCT'C
Cuts at: 1433 2572 2811 3259 3365 4645 1433
Size: 1139 239 448 106 1280 4421
Fragments arranged by size:
4421 1280 1139 448 239 106
SalI G'TCGA_C
Cuts at: 4668 4668
Size: 7633
SanDI GG'GwC_CC
Cuts at: 1844 1948 1844
Size: 104 7529
SapI GCTCTTCn'nnn_
Cuts at: 2089 4562 5654 5864 2089
Size: 2473 1092 210 3858
Fragments arranged by size:
3858 2473 1092 210
SbfI CC_TGCA'GG
Cuts at: 4678 4678
Size: 7633
ScaI AGT'ACT
Cuts at: 7150 7150
Size: 7633
SexAI A'CCwGG_T
Cuts at: 2224 2224
Size: 7633
SfcI C'TryA_G
Cuts at: 33 693 764 1289 1387 1514 2543 2577 2793
Size: 660 71 525 98 127 1029 34 216
Cuts at: 2793 4674 5359 6354 6545 33
Size: 1881 685 995 191 1121
Fragments arranged by size:
1881 1121 1029 995 685 660 525 216
191 127 98 71 34
SfoI GGC'GCC
Cuts at: 2593 3323 5063 5311 2593
Size: 730 1740 248 4915
Fragments arranged by size:
4915 1740 730 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7633
SmaI CCC'GGG
Cuts at: 1849 3337 4303 4653 1849
Size: 1488 966 350 4829
Fragments arranged by size:
4829 1488 966 350
SmlI C'TyrA_G
Cuts at: 199 376 2402 2962 3139 3720 4786 5701 6195
Size: 177 2026 560 177 581 1066 915 494
Cuts at: 6195 6457 199
Size: 262 1375
Fragments arranged by size:
2026 1375 1066 915 581 560 494 262
177 177
SnaBI TAC'GTA
Cuts at: 4623 4623
Size: 7633
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7633
SphI G_CATG'C
Cuts at: 4684 5184 4684
Size: 500 7133
SspI AAT'ATT
Cuts at: 3975 3975
Size: 7633
StuI AGG'CCT
Cuts at: 979 1210 2196 3418 979
Size: 231 986 1222 5194
Fragments arranged by size:
5194 1222 986 231
StyI C'CwwG_G
Cuts at: 125 1805 2348 2600 3195 3986 4041 4173 4403
Size: 1680 543 252 595 791 55 132 230
Cuts at: 4403 4765 5119 125
Size: 362 354 2639
Fragments arranged by size:
2639 1680 791 595 543 362 354 252
230 132 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1813 3293 5214 5347 5806 6017 1813
Size: 1480 1921 133 459 211 3429
Fragments arranged by size:
3429 1921 1480 459 211 133
TatI w'GTAC_w
Cuts at: 3458 7148 3458
Size: 3690 3943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 7150 7182 3725
Size: 32 3393 32 4176
Fragments arranged by size:
4176 3393 32 32
Tth111I GACn'n_nGTC
Cuts at: 1464 3387 5428 1464
Size: 1923 2041 3669
Fragments arranged by size:
3669 2041 1923
XbaI T'CTAG_A
Cuts at: 2721 4662 2721
Size: 1941 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 997 4243 5126 597
Size: 400 3246 883 3104
Fragments arranged by size:
3246 3104 883 400
XhoI C'TCGA_G
Cuts at: 2962 3720 2962
Size: 758 6875
XmaI C'CCGG_G
Cuts at: 1847 3335 4301 4651 1847
Size: 1488 966 350 4829
Fragments arranged by size:
4829 1488 966 350
XmnI GAAnn'nnTTC
Cuts at: 4113 4113
Size: 7633
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI BaeI BcgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NotI PacI PshAI PsiI
PsrI SacII SfiI SgrAI SrfI SwaI ZraI