Restriction map for
pF1KSDA1215
(Circular) (Six-base) MAPSORT of: pF1KSDA1215.seq Check: 9077 from: 1 to: 4700
pF1KSDA1215 4700 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 980 1738 980
Size: 758 3942
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 911 4540 911
Size: 3629 1071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1563 2525 2957 3704 3972 1563
Size: 962 432 747 268 2291
Fragments arranged by size:
2291 962 747 432 268
AflIII A'CryG_T
Cuts at: 2052 3156 4133 2052
Size: 1104 977 2619
Fragments arranged by size:
2619 1104 977
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AhdI GACnn_n'nnGTC
Cuts at: 135 294 135
Size: 159 4541
AleI CACnn'nnGTG
Cuts at: 446 522 446
Size: 76 4624
AlwNI CAG_nnn'CTG
Cuts at: 2005 3572 2005
Size: 1567 3133
ApaLI G'TGCA_C
Cuts at: 811 3470 811
Size: 2659 2041
ApoI r'AATT_y
Cuts at: 1399 1630 1704 4053 4309 1399
Size: 231 74 2349 256 1790
Fragments arranged by size:
2349 1790 256 231 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 147 271 746 1094 1286 1720 147
Size: 124 475 348 192 434 3127
Fragments arranged by size:
3127 475 434 348 192 124
BamHI G'GATC_C
Cuts at: 947 1638 1725 947
Size: 691 87 3922
Fragments arranged by size:
3922 691 87
BanI G'GyrC_C
Cuts at: 355 892 1716 2130 2378 2413 355
Size: 537 824 414 248 35 2642
Fragments arranged by size:
2642 824 537 414 248 35
BanII G_rGCy'C
Cuts at: 281 621 1714 2744 281
Size: 340 1093 1030 2237
Fragments arranged by size:
2237 1093 1030 340
BbeI G_GCGC'C
Cuts at: 896 2134 2382 896
Size: 1238 248 3214
Fragments arranged by size:
3214 1238 248
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglI GCCn_nnn'nGGC
Cuts at: 469 469
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1163 1812 1163
Size: 649 4051
Bme1580I G_kGCm'C
Cuts at: 358 675 815 2325 2418 3474 358
Size: 317 140 1510 93 1056 1584
Fragments arranged by size:
1584 1510 1056 317 140 93
BmrI ACTGGGnnnn_n'
Cuts at: 135 336 616 1912 1993 2328 4472 135
Size: 201 280 1296 81 335 2144 363
Fragments arranged by size:
2144 1296 363 335 280 201 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 835 856 973 559
Size: 276 21 117 4286
Fragments arranged by size:
4286 276 117 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1525 1876 2755 3247 3545 853
Size: 672 351 879 492 298 2008
Fragments arranged by size:
2008 879 672 492 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 711 1672 4404 711
Size: 961 2732 1007
Fragments arranged by size:
2732 1007 961
BsaAI yAC'GTr
Cuts at: 1692 2053 1692
Size: 361 4339
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 797 893 1423 2131 2379 3081 4577 797
Size: 96 530 708 248 702 1496 920
Fragments arranged by size:
1496 920 708 702 530 248 96
BsaWI w'CCGG_w
Cuts at: 1889 2410 3088 3362 3509 3920 1889
Size: 521 678 274 147 411 2669
Fragments arranged by size:
2669 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 358
Size: 30 4670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 985 1171 1203 1231 1267 1534 547
Size: 438 186 32 28 36 267 3713
Fragments arranged by size:
3713 438 267 186 36 32 28
BseYI C'CCAG_C
Cuts at: 614 2125 2883 3460 3956 614
Size: 1511 758 577 496 1358
Fragments arranged by size:
1511 1358 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 793
Size: 4700
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 368 815 1714 2492 2682 3474 368
Size: 447 899 778 190 792 1594
Fragments arranged by size:
1594 899 792 778 447 190
Bsp1286I G_dGCh'C
Cuts at: 281 358 368 621 675 815 1714 2325 2418
Size: 77 10 253 54 140 899 611 93
Cuts at: 2418 2492 2682 2744 3474 281
Size: 74 190 62 730 1507
Fragments arranged by size:
1507 899 730 611 253 190 140 93
77 74 62 54 10
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 1661 3876 1661
Size: 2215 2485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1010 1750 1964 2266 2647 1010
Size: 740 214 302 381 3063
Fragments arranged by size:
3063 740 381 302 214
BsrBI CCG'CTC
Cuts at: 50 218 315 2992 3046 4530 4695 50
Size: 168 97 2677 54 1484 165 55
Fragments arranged by size:
2677 1484 168 165 97 55 54
BsrDI GCAATG_nn'
Cuts at: 2612 2612
Size: 4700
BsrFI r'CCGG_y
Cuts at: 632 1524 2698 2879 3088 632
Size: 892 1174 181 209 2244
Fragments arranged by size:
2244 1174 892 209 181
BssSI C'ACGA_G
Cuts at: 302 700 2971 3329 302
Size: 398 2271 358 1673
Fragments arranged by size:
2271 1673 398 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 110 3061 110
Size: 2951 1749
BstEII G'GTnAC_C
Cuts at: 1527 1527
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 408 524 408
Size: 116 4584
BstYI r'GATC_y
Cuts at: 1 947 1638 1725 2192 2215 2550 2796 3808
Size: 946 691 87 467 23 335 246 1012
Cuts at: 3808 3894 3906 4252 1
Size: 86 12 346 449
Fragments arranged by size:
1012 946 691 467 449 346 335 246
87 86 23 12
BtgI C'CryG_G
Cuts at: 125 824 1316 1373 125
Size: 699 492 57 3452
Fragments arranged by size:
3452 699 492 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 381 2650 2831 2994 381
Size: 2269 181 163 2087
Fragments arranged by size:
2269 2087 181 163
BtsI GCAGTG_nn'
Cuts at: 472 517 947 1943 472
Size: 45 430 996 3229
Fragments arranged by size:
3229 996 430 45
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 976 2406 3264 976
Size: 1430 858 2412
Fragments arranged by size:
2412 1430 858
EaeI y'GGCC_r
Cuts at: 821 915 1019 1086 1493 2285 2459 2850 2877
Size: 94 104 67 407 792 174 391 27
Cuts at: 2877 4222 821
Size: 1345 1299
Fragments arranged by size:
1345 1299 792 407 391 174 104 94
67 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 574 724 1244 2723 2933 574
Size: 150 520 1479 210 2341
Fragments arranged by size:
2341 1479 520 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 619 2908 3218 3364 4510 619
Size: 2289 310 146 1146 809
Fragments arranged by size:
2289 1146 809 310 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 835 856 973 1563 2525 2957 3704 3972
Size: 276 21 117 590 962 432 747 268
Cuts at: 3972 559
Size: 1287
Fragments arranged by size:
1287 962 747 590 432 276 268 117
21
EcoNI CCTnn'n_nnAGG
Cuts at: 1057 1057
Size: 4700
EcoO109I rG'GnC_Cy
Cuts at: 564 1605 1839 564
Size: 1041 234 3425
Fragments arranged by size:
3425 1041 234
EcoRI G'AATT_C
Cuts at: 1399 1704 1399
Size: 305 4395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1452 2719 1452
Size: 1267 3433
FspI TGC'GCA
Cuts at: 733 1183 2481 733
Size: 450 1298 2952
Fragments arranged by size:
2952 1298 450
FspAI rTGC'GCAy
Cuts at: 733 733
Size: 4700
HaeII r_GCGC'y
Cuts at: 896 2134 2382 3404 4383 896
Size: 1238 248 1022 979 1213
Fragments arranged by size:
1238 1213 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 280 312 1451 1483 1630 1662 2542
Size: 32 127 32 1139 32 147 32 880
Cuts at: 2542 2572 2574 2604 121
Size: 30 2 30 2217
Fragments arranged by size:
2217 1139 880 147 127 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
KasI G'GCGC_C
Cuts at: 892 2130 2378 892
Size: 1238 248 3214
Fragments arranged by size:
3214 1238 248
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3371 3555 3371
Size: 184 4516
MscI TGG'CCA
Cuts at: 917 1021 1088 1495 2461 917
Size: 104 67 407 966 3156
Fragments arranged by size:
3156 966 407 104 67
MslI CAynn'nnrTG
Cuts at: 446 522 2689 2816 446
Size: 76 2167 127 2330
Fragments arranged by size:
2330 2167 127 76
MspA1I CmG'CkG
Cuts at: 457 614 1111 1811 2125 2485 3498 3743 457
Size: 157 497 700 314 360 1013 245 1414
Fragments arranged by size:
1414 1013 700 497 360 314 245 157
NaeI GCC'GGC
Cuts at: 634 2881 634
Size: 2247 2453
NarI GG'CG_CC
Cuts at: 893 2131 2379 893
Size: 1238 248 3214
Fragments arranged by size:
3214 1238 248
NcoI C'CATG_G
Cuts at: 125 1316 1373 125
Size: 1191 57 3452
Fragments arranged by size:
3452 1191 57
NgoMIV G'CCGG_C
Cuts at: 632 2879 632
Size: 2247 2453
NspI r_CATG'y
Cuts at: 1753 2253 3160 1753
Size: 500 907 3293
Fragments arranged by size:
3293 907 500
PasI CC'CwG_GG
Cuts at: 1268 1268
Size: 4700
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 768 936 1374 1502 768
Size: 168 438 128 3966
Fragments arranged by size:
3966 438 168 128
PfoI T'CCnGG_A
Cuts at: 737 950 737
Size: 213 4487
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 1605 1605
Size: 4700
PshAI GACnn'nnGTC
Cuts at: 1133 1133
Size: 4700
PstI C_TGCA'G
Cuts at: 1247 1391 1673 1747 2432 1247
Size: 144 282 74 685 3515
Fragments arranged by size:
3515 685 282 144 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 614 2125 2485 614
Size: 1511 360 2829
Fragments arranged by size:
2829 1511 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 724 2723 2933 724
Size: 1999 210 2491
Fragments arranged by size:
2491 1999 210
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 145 1147 4217 145
Size: 1002 3070 628
Fragments arranged by size:
3070 1002 628
SfcI C'TryA_G
Cuts at: 33 1243 1387 1669 1743 2428 3421 3612 33
Size: 1210 144 282 74 685 993 191 1121
Fragments arranged by size:
1210 1121 993 685 282 191 144 74
SfoI GGC'GCC
Cuts at: 894 2132 2380 894
Size: 1238 248 3214
Fragments arranged by size:
3214 1238 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 273 1288 1722 273
Size: 1015 434 3251
Fragments arranged by size:
3251 1015 434
SmlI C'TyrA_G
Cuts at: 868 1540 1855 2770 3262 3524 868
Size: 672 315 915 492 262 2044
Fragments arranged by size:
2044 915 672 492 315 262
SnaBI TAC'GTA
Cuts at: 1692 1692
Size: 4700
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StuI AGG'CCT
Cuts at: 643 1222 1432 643
Size: 579 210 3911
Fragments arranged by size:
3911 579 210
StyI C'CwwG_G
Cuts at: 125 401 638 1316 1373 1427 1834 2188 125
Size: 276 237 678 57 54 407 354 2637
Fragments arranged by size:
2637 678 407 354 276 237 57 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 224 436 2283 2416 2875 3084 224
Size: 212 1847 133 459 209 1840
Fragments arranged by size:
1847 1840 459 212 209 133
TatI w'GTAC_w
Cuts at: 143 1145 4215 143
Size: 1002 3070 628
Fragments arranged by size:
3070 1002 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 4217 4249 358
Size: 32 3827 32 809
Fragments arranged by size:
3827 809 32 32
Tth111I GACn'n_nGTC
Cuts at: 418 2497 418
Size: 2079 2621
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 1203 1275 1434 2195 1014
Size: 189 72 159 761 3519
Fragments arranged by size:
3519 761 189 159 72
XmaI C'CCGG_G
Cuts at: 271 1286 1720 271
Size: 1015 434 3251
Fragments arranged by size:
3251 1015 434
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI ZraI