Restriction map for
pF1KSDA1217
(Circular) (Six-base) MAPSORT of: pF1KSDA1217.seq Check: 8446 from: 1 to: 6931
pF1KSDA1217 6931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3582 3636 3582
Size: 54 6877
AatII G_ACGT'C
Cuts at: 316 2324 316
Size: 2008 4923
AccI GT'mk_AC
Cuts at: 832 1487 3098 3967 832
Size: 655 1611 869 3796
Fragments arranged by size:
3796 1611 869 655
Acc65I G'GTAC_C
Cuts at: 3945 3945
Size: 6931
AclI AA'CG_TT
Cuts at: 6771 6771
Size: 6931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 2284 2317 2452 3185 4754 5186 5935 6203
Size: 2088 33 135 733 1569 432 749 268
Cuts at: 6203 196
Size: 924
Fragments arranged by size:
2088 1569 924 749 733 432 268 135
33
AfeI AGC'GCT
Cuts at: 1464 1464
Size: 6931
AflIII A'CryG_T
Cuts at: 2343 4281 5387 6364 2343
Size: 1938 1106 977 2910
Fragments arranged by size:
2910 1938 1106 977
AgeI A'CCGG_T
Cuts at: 5319 5319
Size: 6931
AhdI GACnn_n'nnGTC
Cuts at: 1996 1996
Size: 6931
AleI CACnn'nnGTG
Cuts at: 1438 1438
Size: 6931
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1538 3547 1538
Size: 2009 4922
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1841 1873 3510 3542 1841
Size: 32 1637 32 5230
Fragments arranged by size:
5230 1637 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4234 5803 4234
Size: 1569 5362
ApaI G_GGCC'C
Cuts at: 777 1640 3315 777
Size: 863 1675 4393
Fragments arranged by size:
4393 1675 863
ApaLI G'TGCA_C
Cuts at: 5701 5701
Size: 6931
ApoI r'AATT_y
Cuts at: 819 872 2197 2979 3933 6284 6540 819
Size: 53 1325 782 954 2351 256 1210
Fragments arranged by size:
2351 1325 1210 954 782 256 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 6931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6931
AvaI C'yCGr_G
Cuts at: 3949 3949
Size: 6931
BamHI G'GATC_C
Cuts at: 678 1977 3402 3954 678
Size: 1299 1425 552 3655
Fragments arranged by size:
3655 1425 1299 552
BanI G'GyrC_C
Cuts at: 886 1432 1770 1798 2485 2514 3814 3823 3945
Size: 546 338 28 687 29 1300 9 122
Cuts at: 3945 4359 4607 4642 886
Size: 414 248 35 3175
Fragments arranged by size:
3175 1300 687 546 414 338 248 122
35 29 28 9
BanII G_rGCy'C
Cuts at: 250 777 1288 1640 2360 2554 3304 3315 3943
Size: 527 511 352 720 194 750 11 628
Cuts at: 3943 4973 250
Size: 1030 2208
Fragments arranged by size:
2208 1030 750 720 628 527 511 352
194 11
BbeI G_GCGC'C
Cuts at: 4363 4611 4363
Size: 248 6683
BbsI GAAGACnn'nnnn_
Cuts at: 1022 2078 2090 2147 1022
Size: 1056 12 57 5806
Fragments arranged by size:
5806 1056 57 12
BbvCI CC'TCA_GC
Cuts at: 1803 1906 1803
Size: 103 6828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3312 3346 3312
Size: 34 6897
BciVI GTATCCnnnnn_n'
Cuts at: 4827 5590 6931 4827
Size: 763 1341 4827
Fragments arranged by size:
4827 1341 763
BclI T'GATC_A
Cuts at: 4449 4449
Size: 6931
BfrBI ATG'CAT
Cuts at: 1218 1218
Size: 6931
BglII A'GATC_T
Cuts at: 430 4444 6483 430
Size: 4014 2039 878
Fragments arranged by size:
4014 2039 878
BlpI GC'TnA_GC
Cuts at: 4041 4041
Size: 6931
Bme1580I G_kGCm'C
Cuts at: 777 845 889 1640 1801 2490 3315 3826 4554
Size: 68 44 751 161 689 825 511 728
Cuts at: 4554 4647 5705 777
Size: 93 1058 2003
Fragments arranged by size:
2003 1058 825 751 728 689 511 161
93 68 44
BmgBI CAC'GTC
Cuts at: 2136 2136
Size: 6931
BmrI ACTGGGnnnn_n'
Cuts at: 272 569 1291 2938 4141 4222 4557 6703 272
Size: 297 722 1647 1203 81 335 2146 500
Fragments arranged by size:
2146 1647 1203 722 500 335 297 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 5296 3707
Size: 1589 5342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 821 924 939 1780 1843 1849 1867 1993
Size: 385 103 15 841 63 6 18 126
Cuts at: 1993 2920 3280 3288 436
Size: 927 360 8 4079
Fragments arranged by size:
4079 927 841 385 360 126 103 63
18 15 8 6
Bpu10I CC'TnA_GC
Cuts at: 478 1803 1906 2867 3128 478
Size: 1325 103 961 261 4281
Fragments arranged by size:
4281 1325 961 261 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 4105 4984 5478 5776 1846
Size: 2259 879 494 298 3001
Fragments arranged by size:
3001 2259 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 227 1552 6635 227
Size: 1325 5083 523
Fragments arranged by size:
5083 1325 523
BsaAI yAC'GTr
Cuts at: 2622 3921 4282 2622
Size: 1299 361 5271
Fragments arranged by size:
5271 1299 361
BsaBI GATnn'nnATC
Cuts at: 6 4443 6482 6
Size: 4437 2039 455
Fragments arranged by size:
4437 2039 455
BsaHI Gr'CG_yC
Cuts at: 313 2321 4360 4608 5312 6808 313
Size: 2008 2039 248 704 1496 436
Fragments arranged by size:
2039 2008 1496 704 436 248
BsaWI w'CCGG_w
Cuts at: 1172 1260 4118 4639 5319 5593 5740 6151 1172
Size: 88 2858 521 680 274 147 411 1952
Fragments arranged by size:
2858 1952 680 521 411 274 147 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 922 952 1758 1788 1841 1871 2162 2192 3625
Size: 30 806 30 53 30 291 30 1433
Cuts at: 3625 3655 922
Size: 30 4198
Fragments arranged by size:
4198 1433 806 291 53 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 649 773 1298 1490 2284 2458 3014 3017 3029
Size: 124 525 192 794 174 556 3 12
Cuts at: 3029 3205 3228 3231 3268 3762 3783 649
Size: 176 23 3 37 494 21 3797
Fragments arranged by size:
3797 794 556 525 494 192 176 174
124 37 23 21 12 3 3
BseYI C'CCAG_C
Cuts at: 1777 2576 2634 3003 3508 3844 4354 5112 5691
Size: 799 58 369 505 336 510 758 579
Cuts at: 5691 6187 1777
Size: 496 2521
Fragments arranged by size:
2521 799 758 579 510 505 496 369
336 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 668 1736 2005 524
Size: 144 1068 269 5450
Fragments arranged by size:
5450 1068 269 144
BsiEI CG_ry'CG
Cuts at: 104 296 4471 4517 5727 104
Size: 192 4175 46 1210 1308
Fragments arranged by size:
4175 1308 1210 192 46
BsiHKAI G_wGCw'C
Cuts at: 522 1758 2554 3750 3943 4721 4911 5705 522
Size: 1236 796 1196 193 778 190 794 1748
Fragments arranged by size:
1748 1236 1196 796 794 778 193 190
BsmBI CGTCTCn'nnnn_
Cuts at: 304 3804 304
Size: 3500 3431
Bsp1286I G_dGCh'C
Cuts at: 250 522 777 845 889 1288 1640 1758 1801
Size: 272 255 68 44 399 352 118 43
Cuts at: 1801 2360 2490 2554 3304 3315 3750 3826 3943
Size: 559 130 64 750 11 435 76 117
Cuts at: 3943 4554 4647 4721 4911 4973 5705 250
Size: 611 93 74 190 62 732 1476
Fragments arranged by size:
1476 750 732 611 559 435 399 352
272 255 190 130 118 117 93 76
74 68 64 62 44 43 11
BspEI T'CCGG_A
Cuts at: 1260 6151 1260
Size: 4891 2040
BspHI T'CATG_A
Cuts at: 2031 2879 6107 2031
Size: 848 3228 2855
Fragments arranged by size:
3228 2855 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3062 3489 3582 3636 3979 4193 4495 4876 3062
Size: 427 93 54 343 214 302 381 5117
Fragments arranged by size:
5117 427 381 343 302 214 93 54
BsrBI CCG'CTC
Cuts at: 50 893 5221 5275 6761 6926 50
Size: 843 4328 54 1486 165 55
Fragments arranged by size:
4328 1486 843 165 55 54
BsrDI GCAATG_nn'
Cuts at: 918 1034 1046 1148 1919 4841 918
Size: 116 12 102 771 2922 3008
Fragments arranged by size:
3008 2922 771 116 102 12
BsrFI r'CCGG_y
Cuts at: 3103 4927 5108 5319 3103
Size: 1824 181 211 4715
Fragments arranged by size:
4715 1824 211 181
BsrGI T'GTAC_A
Cuts at: 668 1043 1235 668
Size: 375 192 6364
Fragments arranged by size:
6364 375 192
BssSI C'ACGA_G
Cuts at: 383 1084 3078 5200 5560 383
Size: 701 1994 2122 360 1754
Fragments arranged by size:
2122 1994 1754 701 360
BstAPI GCAn_nnn'nTGC
Cuts at: 4306 4306
Size: 6931
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6931
BstEII G'GTnAC_C
Cuts at: 214 286 3706 214
Size: 72 3420 3439
Fragments arranged by size:
3439 3420 72
BstXI CCAn_nnnn'nTGG
Cuts at: 1735 2118 1735
Size: 383 6548
BstYI r'GATC_y
Cuts at: 1 430 678 787 1977 3402 3954 4421 4444
Size: 429 248 109 1190 1425 552 467 23
Cuts at: 4444 4779 5025 6039 6125 6137 6483 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1425 1190 1014 552 467 449 429 346
335 248 246 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1327 1327
Size: 6931
BtgI C'CryG_G
Cuts at: 896 1113 1362 1456 1580 1766 2102 2585 896
Size: 217 249 94 124 186 336 483 5242
Fragments arranged by size:
5242 483 336 249 217 186 124 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 476 2510 3104 4879 5060 5223 476
Size: 2034 594 1775 181 163 2184
Fragments arranged by size:
2184 2034 1775 594 181 163
BtsI GCAGTG_nn'
Cuts at: 2777 2923 3144 4172 2777
Size: 146 221 1028 5536
Fragments arranged by size:
5536 1028 221 146
ClaI AT'CG_AT
Cuts at: 3589 3589
Size: 6931
DraI TTT'AAA
Cuts at: 1419 1632 3928 1419
Size: 213 2296 4422
Fragments arranged by size:
4422 2296 213
DraIII CAC_nnn'GTG
Cuts at: 2586 2751 2586
Size: 165 6766
DrdI GACnn_nn'nnGTC
Cuts at: 4635 5495 4635
Size: 860 6071
EaeI y'GGCC_r
Cuts at: 2463 2624 3741 4514 4688 5079 5106 6453 2463
Size: 161 1117 773 174 391 27 1347 2941
Fragments arranged by size:
2941 1347 1117 773 391 174 161 27
EagI C'GGCC_G
Cuts at: 4514 4514
Size: 6931
EarI CTCTTCn'nnn_
Cuts at: 145 529 990 1960 2033 2428 3543 4952 5162
Size: 384 461 970 73 395 1115 1409 210
Cuts at: 5162 145
Size: 1914
Fragments arranged by size:
1914 1409 1115 970 461 395 384 210
73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1851 1861 3020 5137 5449 5595 6741 1851
Size: 10 1159 2117 312 146 1146 2041
Fragments arranged by size:
2117 2041 1159 1146 312 146 10
EcoICRI GAG'CTC
Cuts at: 2552 3941 2552
Size: 1389 5542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 436 821 924 939 1780 1843 1849 1867
Size: 240 385 103 15 841 63 6 18
Cuts at: 1867 1993 2284 2317 2452 2920 3185 3280 3288
Size: 126 291 33 135 468 265 95 8
Cuts at: 3288 4754 5186 5935 6203 196
Size: 1466 432 749 268 924
Fragments arranged by size:
1466 924 841 749 468 432 385 291
268 265 240 135 126 103 95 63
33 18 15 8 6
EcoO109I rG'GnC_Cy
Cuts at: 773 774 1636 3311 3312 4068 773
Size: 1 862 1675 1 756 3636
Fragments arranged by size:
3636 1675 862 756 1 1
EcoRI G'AATT_C
Cuts at: 872 3933 872
Size: 3061 3870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4948 4948
Size: 6931
FspI TGC'GCA
Cuts at: 2236 2308 4710 2236
Size: 72 2402 4457
Fragments arranged by size:
4457 2402 72
HaeII r_GCGC'y
Cuts at: 983 1466 4363 4611 5635 6614 983
Size: 483 2897 248 1024 979 1300
Fragments arranged by size:
2897 1300 1024 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 644 676 922 954 1381 1413 1982
Size: 32 323 32 246 32 427 32 569
Cuts at: 1982 2014 2488 2520 3053 3085 4771 4801 4803
Size: 32 474 32 533 32 1686 30 2
Cuts at: 4803 4833 289
Size: 30 2387
Fragments arranged by size:
2387 1686 569 533 474 427 323 246
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 410 3968 410
Size: 3558 3373
KasI G'GCGC_C
Cuts at: 4359 4607 4359
Size: 248 6683
KpnI G_GTAC'C
Cuts at: 3949 3949
Size: 6931
MluI A'CGCG_T
Cuts at: 6364 6364
Size: 6931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 379 2048 2227 2486 5602 5786 379
Size: 1669 179 259 3116 184 1524
Fragments arranged by size:
3116 1669 1524 259 184 179
MscI TGG'CCA
Cuts at: 2626 4690 2626
Size: 2064 4867
MslI CAynn'nnrTG
Cuts at: 559 730 1039 1355 1438 1813 2242 2401 2831
Size: 171 309 316 83 375 429 159 430
Cuts at: 2831 4918 5045 559
Size: 2087 127 2445
Fragments arranged by size:
2445 2087 430 429 375 316 309 171
159 127 83
MspA1I CmG'CkG
Cuts at: 319 1887 3308 4040 4354 4714 5729 5974 319
Size: 1568 1421 732 314 360 1015 245 1276
Fragments arranged by size:
1568 1421 1276 1015 732 360 314 245
NaeI GCC'GGC
Cuts at: 5110 5110
Size: 6931
NarI GG'CG_CC
Cuts at: 4360 4608 4360
Size: 248 6683
NcoI C'CATG_G
Cuts at: 896 1113 1580 896
Size: 217 467 6247
Fragments arranged by size:
6247 467 217
NdeI CA'TA_TG
Cuts at: 1220 1220
Size: 6931
NgoMIV G'CCGG_C
Cuts at: 5108 5108
Size: 6931
NruI TCG'CGA
Cuts at: 5292 5292
Size: 6931
NsiI A_TGCA'T
Cuts at: 1220 1220
Size: 6931
NspI r_CATG'y
Cuts at: 691 1344 2347 3982 4482 5391 691
Size: 653 1003 1635 500 909 2231
Fragments arranged by size:
2231 1635 1003 909 653 500
PasI CC'CwG_GG
Cuts at: 769 926 769
Size: 157 6774
PciI A'CATG_T
Cuts at: 2343 5387 2343
Size: 3044 3887
PmeI GTTT'AAAC
Cuts at: 3928 3928
Size: 6931
PmlI CAC'GTG
Cuts at: 2622 2622
Size: 6931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1841 1873 6070 6102 1841
Size: 32 4197 32 2670
Fragments arranged by size:
4197 2670 32 32
PshAI GACnn'nnGTC
Cuts at: 2320 2320
Size: 6931
PspOMI G'GGCC_C
Cuts at: 773 1636 3311 773
Size: 863 1675 4393
Fragments arranged by size:
4393 1675 863
PstI C_TGCA'G
Cuts at: 1470 1892 2669 3401 3976 4661 1470
Size: 422 777 732 575 685 3740
Fragments arranged by size:
3740 777 732 685 575 422
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6931
PvuII CAG'CTG
Cuts at: 1887 4354 4714 1887
Size: 2467 360 4104
Fragments arranged by size:
4104 2467 360
RsrII CG'GwC_CG
Cuts at: 5124 5124
Size: 6931
SacI G_AGCT'C
Cuts at: 2554 3943 2554
Size: 1389 5542
SalI G'TCGA_C
Cuts at: 3966 3966
Size: 6931
SapI GCTCTTCn'nnn_
Cuts at: 4952 5162 4952
Size: 210 6721
SbfI CC_TGCA'GG
Cuts at: 3976 3976
Size: 6931
ScaI AGT'ACT
Cuts at: 6448 6448
Size: 6931
SfcI C'TryA_G
Cuts at: 33 1466 1527 1888 2177 2665 3397 3972 4657
Size: 1433 61 361 289 488 732 575 685
Cuts at: 4657 5652 5843 33
Size: 995 191 1121
Fragments arranged by size:
1433 1121 995 732 685 575 488 361
289 191 61
SfoI GGC'GCC
Cuts at: 4361 4609 4361
Size: 248 6683
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6931
SmaI CCC'GGG
Cuts at: 3951 3951
Size: 6931
SmlI C'TyrA_G
Cuts at: 1825 4084 4999 5493 5755 1825
Size: 2259 915 494 262 3001
Fragments arranged by size:
3001 2259 915 494 262
SnaBI TAC'GTA
Cuts at: 3921 3921
Size: 6931
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6931
SphI G_CATG'C
Cuts at: 3982 4482 3982
Size: 500 6431
StuI AGG'CCT
Cuts at: 1414 1414
Size: 6931
StyI C'CwwG_G
Cuts at: 896 1113 1436 1580 2929 3818 4063 4417 896
Size: 217 323 144 1349 889 245 354 3410
Fragments arranged by size:
3410 1349 889 354 323 245 217 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 291 1139 4512 4645 5104 5315 274
Size: 17 848 3373 133 459 211 1890
Fragments arranged by size:
3373 1890 848 459 211 133 17
TatI w'GTAC_w
Cuts at: 668 1043 1235 2393 6446 668
Size: 375 192 1158 4053 1153
Fragments arranged by size:
4053 1158 1153 375 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 1758 1790 3625 3657 6448 6480 1118
Size: 32 608 32 1835 32 2791 32 1569
Fragments arranged by size:
2791 1835 1569 608 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 292 4726 292
Size: 4434 2497
XbaI T'CTAG_A
Cuts at: 3960 3960
Size: 6931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 1735 4424 561
Size: 1174 2689 3068
Fragments arranged by size:
3068 2689 1174
XmaI C'CCGG_G
Cuts at: 3949 3949
Size: 6931
ZraI GAC'GTC
Cuts at: 314 2322 314
Size: 2008 4923
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PasI PciI PmeI
PmlI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BglI BmtI BsiWI BsmI
BssHII BstZ17I CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI PacI PflMI PfoI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI