Restriction map for
pF1KSDA1218
(Circular) (Six-base) MAPSORT of: pF1KSDA1218.seq Check: 5950 from: 1 to: 5638
pF1KSDA1218 5638 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 988 988
Size: 5638
AccI GT'mk_AC
Cuts at: 420 2674 420
Size: 2254 3384
Acc65I G'GTAC_C
Cuts at: 2652 2652
Size: 5638
AclI AA'CG_TT
Cuts at: 5478 5478
Size: 5638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1658 3461 3893 4642 4910 1154
Size: 504 1803 432 749 268 1882
Fragments arranged by size:
1882 1803 749 504 432 268
AflIII A'CryG_T
Cuts at: 2988 4094 5071 2988
Size: 1106 977 3555
Fragments arranged by size:
3555 1106 977
AgeI A'CCGG_T
Cuts at: 1320 2475 4026 1320
Size: 1155 1551 2932
Fragments arranged by size:
2932 1551 1155
AleI CACnn'nnGTG
Cuts at: 1551 1551
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 353 631 718 2941 4510 353
Size: 278 87 2223 1569 1481
Fragments arranged by size:
2223 1569 1481 278 87
ApaLI G'TGCA_C
Cuts at: 2140 4408 2140
Size: 2268 3370
ApoI r'AATT_y
Cuts at: 650 827 910 1542 2640 4991 5247 650
Size: 177 83 632 1098 2351 256 1041
Fragments arranged by size:
2351 1098 1041 632 256 177 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 5638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5638
AvaI C'yCGr_G
Cuts at: 946 1070 1689 1761 2388 2656 946
Size: 124 619 72 627 268 3928
Fragments arranged by size:
3928 627 619 268 124 72
BamHI G'GATC_C
Cuts at: 942 2661 942
Size: 1719 3919
BanI G'GyrC_C
Cuts at: 676 865 2478 2652 3066 3314 3349 676
Size: 189 1613 174 414 248 35 2965
Fragments arranged by size:
2965 1613 414 248 189 174 35
BanII G_rGCy'C
Cuts at: 1180 1310 2167 2189 2650 3680 1180
Size: 130 857 22 461 1030 3138
Fragments arranged by size:
3138 1030 857 461 130 22
BbeI G_GCGC'C
Cuts at: 3070 3318 3070
Size: 248 5390
BbsI GAAGACnn'nnnn_
Cuts at: 408 1160 1979 2330 408
Size: 752 819 351 3716
Fragments arranged by size:
3716 819 752 351
BbvCI CC'TCA_GC
Cuts at: 1813 1813
Size: 5638
BciVI GTATCCnnnnn_n'
Cuts at: 3534 4297 5638 3534
Size: 763 1341 3534
Fragments arranged by size:
3534 1341 763
BclI T'GATC_A
Cuts at: 3156 3156
Size: 5638
BglII A'GATC_T
Cuts at: 809 3151 5190 809
Size: 2342 2039 1257
Fragments arranged by size:
2342 2039 1257
BlpI GC'TnA_GC
Cuts at: 997 2748 997
Size: 1751 3887
Bme1580I G_kGCm'C
Cuts at: 152 2144 2285 2483 3261 3354 4412 152
Size: 1992 141 198 778 93 1058 1378
Fragments arranged by size:
1992 1378 1058 778 198 141 93
BmrI ACTGGGnnnn_n'
Cuts at: 146 1649 2848 2929 3264 5410 146
Size: 1503 1199 81 335 2146 374
Fragments arranged by size:
2146 1503 1199 374 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1351 4003 1351
Size: 2652 2986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 428 549 1286 2203 419
Size: 9 121 737 917 3854
Fragments arranged by size:
3854 917 737 121 9
Bpu10I CC'TnA_GC
Cuts at: 1813 1813
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 3691 4185 4483 2812
Size: 879 494 298 3967
Fragments arranged by size:
3967 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5342 5342
Size: 5638
BsaAI yAC'GTr
Cuts at: 2628 2989 2628
Size: 361 5277
BsaBI GATnn'nnATC
Cuts at: 6 3150 5189 6
Size: 3144 2039 455
Fragments arranged by size:
3144 2039 455
BsaHI Gr'CG_yC
Cuts at: 197 3067 3315 4019 5515 197
Size: 2870 248 704 1496 320
Fragments arranged by size:
2870 1496 704 320 248
BsaWI w'CCGG_w
Cuts at: 156 1320 2475 2825 3346 4026 4300 4447 4858
Size: 1164 1155 350 521 680 274 147 411
Cuts at: 4858 156
Size: 936
Fragments arranged by size:
1164 1155 936 680 521 411 350 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2485 2515 2485
Size: 30 5608
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 714 1033 1989 1992 1995 1998 2001 2004 2007
Size: 319 956 3 3 3 3 3 3
Cuts at: 2007 2493 714
Size: 486 3859
Fragments arranged by size:
3859 956 486 319 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 315 2461 3061 3819 4398 4894 315
Size: 2146 600 758 579 496 1059
Fragments arranged by size:
2146 1059 758 600 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 366
Size: 5638
BsiEI CG_ry'CG
Cuts at: 104 3178 3224 4434 104
Size: 3074 46 1210 1308
Fragments arranged by size:
3074 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1180 2144 2650 3428 3618 4412 1180
Size: 964 506 778 190 794 2406
Fragments arranged by size:
2406 964 794 778 506 190
BsmI GAATG_Cn'
Cuts at: 2088 2088
Size: 5638
BsmBI CGTCTCn'nnnn_
Cuts at: 750 2048 750
Size: 1298 4340
Bsp1286I G_dGCh'C
Cuts at: 152 1180 1310 2144 2167 2189 2285 2483 2650
Size: 1028 130 834 23 22 96 198 167
Cuts at: 2650 3261 3354 3428 3618 3680 4412 152
Size: 611 93 74 190 62 732 1378
Fragments arranged by size:
1378 1028 834 732 611 198 190 167
130 96 93 74 62 23 22
BspEI T'CCGG_A
Cuts at: 156 4858 156
Size: 4702 936
BspHI T'CATG_A
Cuts at: 4814 4814
Size: 5638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 988 2502 2686 2900 3202 3583 988
Size: 1514 184 214 302 381 3043
Fragments arranged by size:
3043 1514 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 3928 3982 5468 5633 50
Size: 3878 54 1486 165 55
Fragments arranged by size:
3878 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3548 3548
Size: 5638
BsrFI r'CCGG_y
Cuts at: 1143 1320 2475 3634 3815 4026 1143
Size: 177 1155 1159 181 211 2755
Fragments arranged by size:
2755 1159 1155 211 181 177
BssSI C'ACGA_G
Cuts at: 1113 3907 4267 1113
Size: 2794 360 2484
Fragments arranged by size:
2794 2484 360
BstAPI GCAn_nnn'nTGC
Cuts at: 676 718 1207 3013 676
Size: 42 489 1806 3301
Fragments arranged by size:
3301 1806 489 42
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1330 1330
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 1431 1431
Size: 5638
BstYI r'GATC_y
Cuts at: 1 809 942 970 1562 2661 3128 3151 3486
Size: 808 133 28 592 1099 467 23 335
Cuts at: 3486 3732 4746 4832 4844 5190 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1099 1014 808 592 467 449 346 335
246 133 86 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 2190 2190
Size: 5638
BtgI C'CryG_G
Cuts at: 125 1523 2219 125
Size: 1398 696 3544
Fragments arranged by size:
3544 1398 696
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1736 3586 3767 3930 1736
Size: 1850 181 163 3444
Fragments arranged by size:
3444 1850 181 163
BtsI GCAGTG_nn'
Cuts at: 210 354 390 2265 2879 210
Size: 144 36 1875 614 2969
Fragments arranged by size:
2969 1875 614 144 36
DraI TTT'AAA
Cuts at: 2635 2635
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2173 2173
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 3342 4202 3342
Size: 860 4778
EaeI y'GGCC_r
Cuts at: 306 501 1293 1802 3221 3395 3786 3813 5160
Size: 195 792 509 1419 174 391 27 1347
Cuts at: 5160 306
Size: 784
Fragments arranged by size:
1419 1347 792 784 509 391 195 174
27
EagI C'GGCC_G
Cuts at: 3221 3221
Size: 5638
EarI CTCTTCn'nnn_
Cuts at: 230 1707 2025 2624 3659 3869 230
Size: 1477 318 599 1035 210 1999
Fragments arranged by size:
1999 1477 1035 599 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1836 3844 4156 4302 5448 1836
Size: 2008 312 146 1146 2026
Fragments arranged by size:
2026 2008 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1178 2648 1178
Size: 1470 4168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 428 549 1154 1286 1658 2203 3461 3893
Size: 9 121 605 132 372 545 1258 432
Cuts at: 3893 4642 4910 419
Size: 749 268 1147
Fragments arranged by size:
1258 1147 749 605 545 432 372 268
132 121 9
EcoNI CCTnn'n_nnAGG
Cuts at: 596 664 596
Size: 68 5570
EcoO109I rG'GnC_Cy
Cuts at: 1839 2056 2307 2775 1839
Size: 217 251 468 4702
Fragments arranged by size:
4702 468 251 217
EcoRI G'AATT_C
Cuts at: 827 1542 2640 827
Size: 715 1098 3825
Fragments arranged by size:
3825 1098 715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5638
FspI TGC'GCA
Cuts at: 3417 3417
Size: 5638
HaeII r_GCGC'y
Cuts at: 1769 3070 3318 4342 5321 1769
Size: 1301 248 1024 979 2086
Fragments arranged by size:
2086 1301 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 2601 2633 3478 3508 3510 3540 123
Size: 32 2446 32 845 30 2 30 2221
Fragments arranged by size:
2446 2221 845 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2493 2675 2493
Size: 182 5456
HpaI GTT'AAC
Cuts at: 2493 2493
Size: 5638
KasI G'GCGC_C
Cuts at: 3066 3314 3066
Size: 248 5390
KpnI G_GTAC'C
Cuts at: 2656 2656
Size: 5638
MluI A'CGCG_T
Cuts at: 5071 5071
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 1305 4309 4493 242
Size: 1063 3004 184 1387
Fragments arranged by size:
3004 1387 1063 184
MscI TGG'CCA
Cuts at: 308 3397 308
Size: 3089 2549
MslI CAynn'nnrTG
Cuts at: 860 1551 2422 3625 3752 860
Size: 691 871 1203 127 2746
Fragments arranged by size:
2746 1203 871 691 127
MspA1I CmG'CkG
Cuts at: 500 2221 2747 3061 3421 4436 4681 500
Size: 1721 526 314 360 1015 245 1457
Fragments arranged by size:
1721 1457 1015 526 360 314 245
NaeI GCC'GGC
Cuts at: 1145 3817 1145
Size: 2672 2966
NarI GG'CG_CC
Cuts at: 3067 3315 3067
Size: 248 5390
NcoI C'CATG_G
Cuts at: 125 1523 125
Size: 1398 4240
NgoMIV G'CCGG_C
Cuts at: 1143 3815 1143
Size: 2672 2966
NruI TCG'CGA
Cuts at: 3999 3999
Size: 5638
NspI r_CATG'y
Cuts at: 2689 3189 4098 2689
Size: 500 909 4229
Fragments arranged by size:
4229 909 500
PacI TTA_AT'TAA
Cuts at: 764 764
Size: 5638
PciI A'CATG_T
Cuts at: 4094 4094
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1324 1324
Size: 5638
PfoI T'CCnGG_A
Cuts at: 187 187
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4777 4809 4777
Size: 32 5606
PpuMI rG'GwC_Cy
Cuts at: 2307 2307
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 2267 2267
Size: 5638
PspXI vC'TCGA_Gb
Cuts at: 1689 2388 1689
Size: 699 4939
PstI C_TGCA'G
Cuts at: 714 2683 3368 714
Size: 1969 685 2984
Fragments arranged by size:
2984 1969 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5638
PvuII CAG'CTG
Cuts at: 3061 3421 3061
Size: 360 5278
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5638
SacI G_AGCT'C
Cuts at: 1180 2650 1180
Size: 1470 4168
SacII CC_GC'GG
Cuts at: 2222 2222
Size: 5638
SalI G'TCGA_C
Cuts at: 2673 2673
Size: 5638
SapI GCTCTTCn'nnn_
Cuts at: 3659 3869 3659
Size: 210 5428
SbfI CC_TGCA'GG
Cuts at: 2683 2683
Size: 5638
ScaI AGT'ACT
Cuts at: 5155 5155
Size: 5638
SexAI A'CCwGG_T
Cuts at: 359 359
Size: 5638
SfcI C'TryA_G
Cuts at: 33 632 710 2679 3364 4359 4550 33
Size: 599 78 1969 685 995 191 1121
Fragments arranged by size:
1969 1121 995 685 599 191 78
SfoI GGC'GCC
Cuts at: 3068 3316 3068
Size: 248 5390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 2475 2475
Size: 5638
SmaI CCC'GGG
Cuts at: 1072 2658 1072
Size: 1586 4052
SmlI C'TyrA_G
Cuts at: 1689 2388 2791 3706 4200 4462 1689
Size: 699 403 915 494 262 2865
Fragments arranged by size:
2865 915 699 494 403 262
SnaBI TAC'GTA
Cuts at: 2628 2628
Size: 5638
SpeI A'CTAG_T
Cuts at: 67 468 67
Size: 401 5237
SphI G_CATG'C
Cuts at: 2689 3189 2689
Size: 500 5138
StyI C'CwwG_G
Cuts at: 125 1523 2770 3124 125
Size: 1398 1247 354 2639
Fragments arranged by size:
2639 1398 1247 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1239 3219 3352 3811 4022 1239
Size: 1980 133 459 211 2855
Fragments arranged by size:
2855 1980 459 211 133
TatI w'GTAC_w
Cuts at: 471 1233 5153 471
Size: 762 3920 956
Fragments arranged by size:
3920 956 762
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 573 605 1605 1637 1879 1911 5155
Size: 32 176 32 1000 32 242 32 3244
Cuts at: 5155 5187 365
Size: 32 816
Fragments arranged by size:
3244 1000 816 242 176 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3433 3433
Size: 5638
XbaI T'CTAG_A
Cuts at: 2667 2667
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 3131 132
Size: 2999 2639
XhoI C'TCGA_G
Cuts at: 1689 2388 1689
Size: 699 4939
XmaI C'CCGG_G
Cuts at: 1070 2656 1070
Size: 1586 4052
XmnI GAAnn'nnTTC
Cuts at: 1124 1124
Size: 5638
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PacI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NsiI PasI PmlI
PsiI PspOMI PsrI SanDI SfiI SrfI SspI StuI
SwaI ZraI