Restriction map for
pF1KSDA1219
(Circular) (Six-base) MAPSORT of: pF1KSDA1219.seq Check: 3292 from: 1 to: 7610
pF1KSDA1219 7610 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 243 243
Size: 7610
AatII G_ACGT'C
Cuts at: 1366 1366
Size: 7610
AccI GT'mk_AC
Cuts at: 3504 4648 3504
Size: 1144 6466
Acc65I G'GTAC_C
Cuts at: 262 4626 262
Size: 4364 3246
AclI AA'CG_TT
Cuts at: 7450 7450
Size: 7610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 2119 2281 2831 3221 3290 3502 3848 5435
Size: 1865 162 550 390 69 212 346 1587
Cuts at: 5435 5867 6614 6882 254
Size: 432 747 268 982
Fragments arranged by size:
1865 1587 982 747 550 432 390 346
268 212 162 69
AflII C'TTAA_G
Cuts at: 2358 2683 2358
Size: 325 7285
AflIII A'CryG_T
Cuts at: 989 4962 6066 7043 989
Size: 3973 1104 977 1556
Fragments arranged by size:
3973 1556 1104 977
AgeI A'CCGG_T
Cuts at: 5998 5998
Size: 7610
AlwNI CAG_nnn'CTG
Cuts at: 277 558 576 2380 4915 6482 277
Size: 281 18 1804 2535 1567 1405
Fragments arranged by size:
2535 1804 1567 1405 281 18
ApaLI G'TGCA_C
Cuts at: 851 2544 6380 851
Size: 1693 3836 2081
Fragments arranged by size:
3836 2081 1693
ApoI r'AATT_y
Cuts at: 288 1045 1636 1924 3424 3694 3998 4331 4351
Size: 757 591 288 1500 270 304 333 20
Cuts at: 4351 4614 6963 7219 288
Size: 263 2349 256 679
Fragments arranged by size:
2349 1500 757 679 591 333 304 288
270 263 256 20
AseI AT'TA_AT
Cuts at: 20 1970 2122 3776 20
Size: 1950 152 1654 3854
Fragments arranged by size:
3854 1950 1654 152
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7610
AvaI C'yCGr_G
Cuts at: 602 4630 602
Size: 4028 3582
BamHI G'GATC_C
Cuts at: 4635 4635
Size: 7610
BanI G'GyrC_C
Cuts at: 262 1280 2128 2435 4626 5040 5288 5323 262
Size: 1018 848 307 2191 414 248 35 2549
Fragments arranged by size:
2549 2191 1018 848 414 307 248 35
BanII G_rGCy'C
Cuts at: 2321 2387 2474 4404 4624 5654 2321
Size: 66 87 1930 220 1030 4277
Fragments arranged by size:
4277 1930 1030 220 87 66
BbeI G_GCGC'C
Cuts at: 2439 5044 5292 2439
Size: 2605 248 4757
Fragments arranged by size:
4757 2605 248
BbsI GAAGACnn'nnnn_
Cuts at: 1321 1714 3069 3656 1321
Size: 393 1355 587 5275
Fragments arranged by size:
5275 1355 587 393
BbvCI CC'TCA_GC
Cuts at: 1667 1667
Size: 7610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 938 2388 2422 904
Size: 34 1450 34 6092
Fragments arranged by size:
6092 1450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1109 5508 6269 7610 1109
Size: 4399 761 1341 1109
Fragments arranged by size:
4399 1341 1109 761
BclI T'GATC_A
Cuts at: 168 5130 168
Size: 4962 2648
BfrBI ATG'CAT
Cuts at: 1168 2828 1168
Size: 1660 5950
BglII A'GATC_T
Cuts at: 3234 4258 5125 7162 3234
Size: 1024 867 2037 3682
Fragments arranged by size:
3682 2037 1024 867
BlpI GC'TnA_GC
Cuts at: 4722 4722
Size: 7610
Bme1580I G_kGCm'C
Cuts at: 855 2548 3078 5235 5328 6384 855
Size: 1693 530 2157 93 1056 2081
Fragments arranged by size:
2157 2081 1693 1056 530 93
BmrI ACTGGGnnnn_n'
Cuts at: 2424 2719 3139 3382 3758 4073 4822 4903 5238
Size: 295 420 243 376 315 749 81 335
Cuts at: 5238 7382 2424
Size: 2144 2652
Fragments arranged by size:
2652 2144 749 420 376 335 315 295
243 81
BmtI G_CTAG'C
Cuts at: 1562 3845 1562
Size: 2283 5327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2363 2493 5977 2363
Size: 130 3484 3996
Fragments arranged by size:
3996 3484 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 923 1733 2574 2716 2901 4561 825
Size: 98 810 841 142 185 1660 3874
Fragments arranged by size:
3874 1660 841 810 185 142 98
Bpu10I CC'TnA_GC
Cuts at: 1667 1667
Size: 7610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3361 4045 4567 4786 5665 6157 6455 2333
Size: 1028 684 522 219 879 492 298 3488
Fragments arranged by size:
3488 1028 879 684 522 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 1428 2334 2510 2890 7314 1428
Size: 906 176 380 4424 1724
Fragments arranged by size:
4424 1724 906 380 176
BsaAI yAC'GTr
Cuts at: 4602 4963 4602
Size: 361 7249
BsaBI GATnn'nnATC
Cuts at: 6 5124 7161 6
Size: 5118 2037 455
Fragments arranged by size:
5118 2037 455
BsaHI Gr'CG_yC
Cuts at: 1363 2436 5041 5289 5991 7487 1363
Size: 1073 2605 248 702 1496 1486
Fragments arranged by size:
2605 1496 1486 1073 702 248
BsaWI w'CCGG_w
Cuts at: 1274 2519 2948 4322 4526 4799 5320 5998 6272
Size: 1245 429 1374 204 273 521 678 274
Cuts at: 6272 6419 6830 1274
Size: 147 411 2054
Fragments arranged by size:
2054 1374 1245 678 521 429 411 274
273 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 356 386 4534 4564 356
Size: 30 4148 30 3402
Fragments arranged by size:
4148 3402 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1367 1817 2464 3116 3370 3500 1367
Size: 450 647 652 254 130 5477
Fragments arranged by size:
5477 652 647 450 254 130
BseYI C'CCAG_C
Cuts at: 816 1020 2380 3314 5035 5793 6370 6866 816
Size: 204 1360 934 1721 758 577 496 1560
Fragments arranged by size:
1721 1560 1360 934 758 577 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 2584 175
Size: 2409 5201
BsiEI CG_ry'CG
Cuts at: 104 5152 5198 6406 104
Size: 5048 46 1208 1308
Fragments arranged by size:
5048 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 855 1206 2474 2548 2654 4404 4624 5402 5592
Size: 351 1268 74 106 1750 220 778 190
Cuts at: 5592 6384 855
Size: 792 2081
Fragments arranged by size:
2081 1750 1268 792 778 351 220 190
106 74
BsmI GAATG_Cn'
Cuts at: 1082 1168 2399 3242 1082
Size: 86 1231 843 5450
Fragments arranged by size:
5450 1231 843 86
Bsp1286I G_dGCh'C
Cuts at: 855 1206 2321 2387 2474 2548 2654 3078 4404
Size: 351 1115 66 87 74 106 424 1326
Cuts at: 4404 4624 5235 5328 5402 5592 5654 6384 855
Size: 220 611 93 74 190 62 730 2081
Fragments arranged by size:
2081 1326 1115 730 611 424 351 220
190 106 93 87 74 74 66 62
BspEI T'CCGG_A
Cuts at: 4322 6830 4322
Size: 2508 5102
BspHI T'CATG_A
Cuts at: 6786 6786
Size: 7610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 243 2352 3498 4660 4874 5176 5557 243
Size: 2109 1146 1162 214 302 381 2296
Fragments arranged by size:
2296 2109 1162 1146 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1073 5902 5956 7440 7605 50
Size: 1023 4829 54 1484 165 55
Fragments arranged by size:
4829 1484 1023 165 55 54
BsrDI GCAATG_nn'
Cuts at: 439 683 2186 2990 5522 439
Size: 244 1503 804 2532 2527
Fragments arranged by size:
2532 2527 1503 804 244
BsrFI r'CCGG_y
Cuts at: 5608 5789 5998 5608
Size: 181 209 7220
Fragments arranged by size:
7220 209 181
BssSI C'ACGA_G
Cuts at: 5881 6239 5881
Size: 358 7252
BstAPI GCAn_nnn'nTGC
Cuts at: 4987 4987
Size: 7610
BstBI TT'CG_AA
Cuts at: 110 5971 110
Size: 5861 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 792 2613 4576 792
Size: 1821 1963 3826
Fragments arranged by size:
3826 1963 1821
BstYI r'GATC_y
Cuts at: 1 1960 2595 3234 3570 3799 3984 4258 4635
Size: 1959 635 639 336 229 185 274 377
Cuts at: 4635 5102 5125 5460 5706 6718 6804 6816 7162
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7162 1
Size: 449
Fragments arranged by size:
1959 1012 639 635 467 449 377 346
336 335 274 246 229 185 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 4103 4103
Size: 7610
BtgI C'CryG_G
Cuts at: 323 1604 323
Size: 1281 6329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5560 5741 5904 5560
Size: 181 163 7266
Fragments arranged by size:
7266 181 163
BtsI GCAGTG_nn'
Cuts at: 145 827 838 1417 4853 145
Size: 682 11 579 3436 2902
Fragments arranged by size:
3436 2902 682 579 11
DraI TTT'AAA
Cuts at: 906 3171 3650 4307 4609 906
Size: 2265 479 657 302 3907
Fragments arranged by size:
3907 2265 657 479 302
DraIII CAC_nnn'GTG
Cuts at: 2863 2863
Size: 7610
DrdI GACnn_nn'nnGTC
Cuts at: 5316 6174 5316
Size: 858 6752
EaeI y'GGCC_r
Cuts at: 1414 1805 2572 3190 5195 5369 5760 5787 7132
Size: 391 767 618 2005 174 391 27 1345
Cuts at: 7132 1414
Size: 1892
Fragments arranged by size:
2005 1892 1345 767 618 391 391 174
27
EagI C'GGCC_G
Cuts at: 5195 5195
Size: 7610
EarI CTCTTCn'nnn_
Cuts at: 1656 1712 2070 2257 3197 3275 3364 5633 5843
Size: 56 358 187 940 78 89 2269 210
Cuts at: 5843 1656
Size: 3423
Fragments arranged by size:
3423 2269 940 358 210 187 89 78
56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4477 5818 6128 6274 7420 4477
Size: 1341 310 146 1146 4667
Fragments arranged by size:
4667 1341 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2472 4402 4622 2472
Size: 1930 220 5460
Fragments arranged by size:
5460 1930 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 825 923 1733 2119 2281 2574 2716 2831
Size: 571 98 810 386 162 293 142 115
Cuts at: 2831 2901 3221 3290 3502 3848 4561 5435 5867
Size: 70 320 69 212 346 713 874 432
Cuts at: 5867 6614 6882 254
Size: 747 268 982
Fragments arranged by size:
982 874 810 747 713 571 432 386
346 320 293 268 212 162 142 115
98 70 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2488 4065 2488
Size: 1577 6033
EcoO109I rG'GnC_Cy
Cuts at: 555 4749 555
Size: 4194 3416
EcoRI G'AATT_C
Cuts at: 3424 3998 4614 3424
Size: 574 616 6420
Fragments arranged by size:
6420 616 574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3086 5629 3086
Size: 2543 5067
FspI TGC'GCA
Cuts at: 5391 5391
Size: 7610
HaeII r_GCGC'y
Cuts at: 2439 5044 5292 6314 7293 2439
Size: 2605 248 1022 979 2756
Fragments arranged by size:
2756 2605 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 2047 2079 2256 2288 2587 2619 4059
Size: 32 1888 32 177 32 299 32 1440
Cuts at: 4059 4091 5452 5482 5484 5514 127
Size: 32 1361 30 2 30 2223
Fragments arranged by size:
2223 1888 1440 1361 299 177 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1446 2508 4518 4649 1446
Size: 1062 2010 131 4407
Fragments arranged by size:
4407 2010 1062 131
HindIII A'AGCT_T
Cuts at: 3121 3121
Size: 7610
HpaI GTT'AAC
Cuts at: 1446 1446
Size: 7610
KasI G'GCGC_C
Cuts at: 2435 5040 5288 2435
Size: 2605 248 4757
Fragments arranged by size:
4757 2605 248
KpnI G_GTAC'C
Cuts at: 266 4630 266
Size: 4364 3246
MluI A'CGCG_T
Cuts at: 7043 7043
Size: 7610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 192 1969 3144 3724 4557 6281 6465 192
Size: 1777 1175 580 833 1724 184 1337
Fragments arranged by size:
1777 1724 1337 1175 833 580 184
MscI TGG'CCA
Cuts at: 1807 5371 1807
Size: 3564 4046
MslI CAynn'nnrTG
Cuts at: 182 969 2164 2611 5599 5726 182
Size: 787 1195 447 2988 127 2066
Fragments arranged by size:
2988 2066 1195 787 447 127
MspA1I CmG'CkG
Cuts at: 1154 2377 4539 4721 5035 5395 6408 6653 1154
Size: 1223 2162 182 314 360 1013 245 2111
Fragments arranged by size:
2162 2111 1223 1013 360 314 245 182
NaeI GCC'GGC
Cuts at: 5791 5791
Size: 7610
NarI GG'CG_CC
Cuts at: 2436 5041 5289 2436
Size: 2605 248 4757
Fragments arranged by size:
4757 2605 248
NcoI C'CATG_G
Cuts at: 323 1604 323
Size: 1281 6329
NdeI CA'TA_TG
Cuts at: 882 882
Size: 7610
NgoMIV G'CCGG_C
Cuts at: 5789 5789
Size: 7610
NheI G'CTAG_C
Cuts at: 1558 3841 1558
Size: 2283 5327
NsiI A_TGCA'T
Cuts at: 1170 2830 1170
Size: 1660 5950
NspI r_CATG'y
Cuts at: 993 1755 2828 3757 4663 5163 6070 993
Size: 762 1073 929 906 500 907 2533
Fragments arranged by size:
2533 1073 929 907 906 762 500
PciI A'CATG_T
Cuts at: 989 6066 989
Size: 5077 2533
PflMI CCAn_nnn'nTGG
Cuts at: 361 361
Size: 7610
PfoI T'CCnGG_A
Cuts at: 2033 4568 2033
Size: 2535 5075
PmeI GTTT'AAAC
Cuts at: 4609 4609
Size: 7610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6749 6781 6749
Size: 32 7578
PpuMI rG'GwC_Cy
Cuts at: 555 555
Size: 7610
PsiI TTA'TAA
Cuts at: 1030 1030
Size: 7610
PstI C_TGCA'G
Cuts at: 641 4594 4657 5342 641
Size: 3953 63 685 2909
Fragments arranged by size:
3953 2909 685 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7610
PvuII CAG'CTG
Cuts at: 1154 2377 4539 5035 5395 1154
Size: 1223 2162 496 360 3369
Fragments arranged by size:
3369 2162 1223 496 360
RsrII CG'GwC_CG
Cuts at: 5805 5805
Size: 7610
SacI G_AGCT'C
Cuts at: 2474 4404 4624 2474
Size: 1930 220 5460
Fragments arranged by size:
5460 1930 220
SalI G'TCGA_C
Cuts at: 4647 4647
Size: 7610
SapI GCTCTTCn'nnn_
Cuts at: 1656 2070 5633 5843 1656
Size: 414 3563 210 3423
Fragments arranged by size:
3563 3423 414 210
SbfI CC_TGCA'GG
Cuts at: 4657 4657
Size: 7610
ScaI AGT'ACT
Cuts at: 1332 2693 4218 7127 1332
Size: 1361 1525 2909 1815
Fragments arranged by size:
2909 1815 1525 1361
SfcI C'TryA_G
Cuts at: 33 233 493 637 4148 4470 4590 4653 5338
Size: 200 260 144 3511 322 120 63 685
Cuts at: 5338 6331 6522 33
Size: 993 191 1121
Fragments arranged by size:
3511 1121 993 685 322 260 200 191
144 120 63
SfoI GGC'GCC
Cuts at: 2437 5042 5290 2437
Size: 2605 248 4757
Fragments arranged by size:
4757 2605 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7610
SmaI CCC'GGG
Cuts at: 4632 4632
Size: 7610
SmlI C'TyrA_G
Cuts at: 2348 2358 2683 3340 4024 4582 4765 5680 6172
Size: 10 325 657 684 558 183 915 492
Cuts at: 6172 6434 2348
Size: 262 3524
Fragments arranged by size:
3524 915 684 657 558 492 325 262
183 10
SnaBI TAC'GTA
Cuts at: 4602 4602
Size: 7610
SpeI A'CTAG_T
Cuts at: 67 1399 67
Size: 1332 6278
SphI G_CATG'C
Cuts at: 4663 5163 4663
Size: 500 7110
SspI AAT'ATT
Cuts at: 379 1127 2190 3175 379
Size: 748 1063 985 4814
Fragments arranged by size:
4814 1063 985 748
StyI C'CwwG_G
Cuts at: 323 785 1604 3708 4125 4744 5098 323
Size: 462 819 2104 417 619 354 2835
Fragments arranged by size:
2835 2104 819 619 462 417 354
SwaI ATTT'AAAT
Cuts at: 3171 3171
Size: 7610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 830 2176 2230 2428 4146 5193 5326 5785 5994
Size: 1346 54 198 1718 1047 133 459 209
Cuts at: 5994 830
Size: 2446
Fragments arranged by size:
2446 1718 1346 1047 459 209 198 133
54
TatI w'GTAC_w
Cuts at: 128 745 1330 2691 2756 4216 7125 128
Size: 617 585 1361 65 1460 2909 613
Fragments arranged by size:
2909 1460 1361 617 613 585 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 940 972 1348 1380 7127 7159 206
Size: 32 702 32 376 32 5747 32 657
Fragments arranged by size:
5747 702 657 376 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4513 5407 4513
Size: 894 6716
XbaI T'CTAG_A
Cuts at: 1183 2466 4641 1183
Size: 1283 2175 4152
Fragments arranged by size:
4152 2175 1283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 792 1602 5105 573
Size: 219 810 3503 3078
Fragments arranged by size:
3503 3078 810 219
XmaI C'CCGG_G
Cuts at: 4630 4630
Size: 7610
XmnI GAAnn'nnTTC
Cuts at: 1721 4184 1721
Size: 2463 5147
ZraI GAC'GTC
Cuts at: 1364 1364
Size: 7610
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BglI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI MfeI NotI
NruI PacI PasI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI XhoI