Restriction map for
pF1KSDA1225
(Circular) (Six-base) MAPSORT of: pF1KSDA1225.seq Check: 99 from: 1 to: 7252
pF1KSDA1225 7252 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 174 4288 174
Size: 4114 3138
Acc65I G'GTAC_C
Cuts at: 159 4266 159
Size: 4107 3145
AclI AA'CG_TT
Cuts at: 373 7092 373
Size: 6719 533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 904 1838 1940 2705 3446 4106 5075 5507
Size: 158 934 102 765 741 660 969 432
Cuts at: 5507 6256 6524 746
Size: 749 268 1474
Fragments arranged by size:
1474 969 934 765 749 741 660 432
268 158 102
AflII C'TTAA_G
Cuts at: 1095 1597 1095
Size: 502 6750
AflIII A'CryG_T
Cuts at: 877 1400 4602 5708 6685 877
Size: 523 3202 1106 977 1444
Fragments arranged by size:
3202 1444 1106 977 523
AgeI A'CCGG_T
Cuts at: 5640 5640
Size: 7252
AleI CACnn'nnGTG
Cuts at: 718 718
Size: 7252
AlwNI CAG_nnn'CTG
Cuts at: 1852 3379 4555 6124 1852
Size: 1527 1176 1569 2980
Fragments arranged by size:
2980 1569 1527 1176
ApaLI G'TGCA_C
Cuts at: 6022 6022
Size: 7252
ApoI r'AATT_y
Cuts at: 591 709 869 1269 1560 2226 3210 4254 6605
Size: 118 160 400 291 666 984 1044 2351
Cuts at: 6605 6861 591
Size: 256 982
Fragments arranged by size:
2351 1044 984 982 666 400 291 256
160 118
AseI AT'TA_AT
Cuts at: 20 401 1244 1972 2198 2519 3572 20
Size: 381 843 728 226 321 1053 3700
Fragments arranged by size:
3700 1053 843 728 381 321 226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7252
AvaI C'yCGr_G
Cuts at: 4270 4270
Size: 7252
BamHI G'GATC_C
Cuts at: 3987 4275 3987
Size: 288 6964
BanI G'GyrC_C
Cuts at: 159 1542 4266 4680 4928 4963 159
Size: 1383 2724 414 248 35 2448
Fragments arranged by size:
2724 2448 1383 414 248 35
BanII G_rGCy'C
Cuts at: 1500 3417 4264 5294 1500
Size: 1917 847 1030 3458
Fragments arranged by size:
3458 1917 1030 847
BbeI G_GCGC'C
Cuts at: 4684 4932 4684
Size: 248 7004
BbsI GAAGACnn'nnnn_
Cuts at: 1529 1667 1732 1529
Size: 138 65 7049
Fragments arranged by size:
7049 138 65
BbvCI CC'TCA_GC
Cuts at: 2801 3146 2801
Size: 345 6907
BciVI GTATCCnnnnn_n'
Cuts at: 2573 5148 5911 7252 2573
Size: 2575 763 1341 2573
Fragments arranged by size:
2575 2573 1341 763
BclI T'GATC_A
Cuts at: 1113 2886 4770 1113
Size: 1773 1884 3595
Fragments arranged by size:
3595 1884 1773
BfrBI ATG'CAT
Cuts at: 3541 3541
Size: 7252
BglI GCCn_nnn'nGGC
Cuts at: 3795 3795
Size: 7252
BglII A'GATC_T
Cuts at: 2688 4765 6804 2688
Size: 2077 2039 3136
Fragments arranged by size:
3136 2077 2039
BlpI GC'TnA_GC
Cuts at: 4362 4362
Size: 7252
Bme1580I G_kGCm'C
Cuts at: 4875 4968 6026 4875
Size: 93 1058 6101
Fragments arranged by size:
6101 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 543 2610 4462 4543 4878 7024 543
Size: 2067 1852 81 335 2146 771
Fragments arranged by size:
2146 2067 1852 771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3474 5617 3474
Size: 2143 5109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 3668 3732 2156
Size: 1512 64 5676
Fragments arranged by size:
5676 1512 64
Bpu10I CC'TnA_GC
Cuts at: 2801 3146 2801
Size: 345 6907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 754 1216 1484 4426 5305 5799 6097 595
Size: 159 462 268 2942 879 494 298 1750
Fragments arranged by size:
2942 1750 879 494 462 298 268 159
BsaI GGTCTCn'nnnn_
Cuts at: 184 1366 1645 3492 6956 184
Size: 1182 279 1847 3464 480
Fragments arranged by size:
3464 1847 1182 480 279
BsaAI yAC'GTr
Cuts at: 4242 4603 4242
Size: 361 6891
BsaBI GATnn'nnATC
Cuts at: 6 290 2969 4764 6803 6
Size: 284 2679 1795 2039 455
Fragments arranged by size:
2679 2039 1795 455 284
BsaHI Gr'CG_yC
Cuts at: 4681 4929 5633 7129 4681
Size: 248 704 1496 4804
Fragments arranged by size:
4804 1496 704 248
BsaWI w'CCGG_w
Cuts at: 4439 4960 5640 5914 6061 6472 4439
Size: 521 680 274 147 411 5219
Fragments arranged by size:
5219 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 3134 3464 202
Size: 2932 330 3990
Fragments arranged by size:
3990 2932 330
BseYI C'CCAG_C
Cuts at: 677 3273 4675 5433 6012 6508 677
Size: 2596 1402 758 579 496 1421
Fragments arranged by size:
2596 1421 1402 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3494 3011
Size: 483 6769
BsiEI CG_ry'CG
Cuts at: 104 3315 4792 4838 6048 104
Size: 3211 1477 46 1210 1308
Fragments arranged by size:
3211 1477 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2543 3172 3561 4264 5042 5232 6026 2543
Size: 629 389 703 778 190 794 3769
Fragments arranged by size:
3769 794 778 703 629 389 190
BsmI GAATG_Cn'
Cuts at: 3666 3666
Size: 7252
Bsp1286I G_dGCh'C
Cuts at: 1500 2543 3172 3417 3561 4264 4875 4968 5042
Size: 1043 629 245 144 703 611 93 74
Cuts at: 5042 5232 5294 6026 1500
Size: 190 62 732 2726
Fragments arranged by size:
2726 1043 732 703 629 611 245 190
144 93 74 62
BspEI T'CCGG_A
Cuts at: 6472 6472
Size: 7252
BspHI T'CATG_A
Cuts at: 2292 6428 2292
Size: 4136 3116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2889 4300 4514 4816 5197 2889
Size: 1411 214 302 381 4944
Fragments arranged by size:
4944 1411 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5542 5596 7082 7247 50
Size: 5492 54 1486 165 55
Fragments arranged by size:
5492 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 216 386 2893 5162 216
Size: 170 2507 2269 2306
Fragments arranged by size:
2507 2306 2269 170
BsrFI r'CCGG_y
Cuts at: 5248 5429 5640 5248
Size: 181 211 6860
Fragments arranged by size:
6860 211 181
BsrGI T'GTAC_A
Cuts at: 1629 1797 1629
Size: 168 7084
BssSI C'ACGA_G
Cuts at: 3704 5521 5881 3704
Size: 1817 360 5075
Fragments arranged by size:
5075 1817 360
BstAPI GCAn_nnn'nTGC
Cuts at: 388 4627 388
Size: 4239 3013
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7252
BstYI r'GATC_y
Cuts at: 1 2688 2719 3678 3987 4275 4742 4765 5100
Size: 2687 31 959 309 288 467 23 335
Cuts at: 5100 5346 6360 6446 6458 6804 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
2687 1014 959 467 449 346 335 309
288 246 86 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 3800 3833 3800
Size: 33 7219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5200 5381 5544 5200
Size: 181 163 6908
Fragments arranged by size:
6908 181 163
BtsI GCAGTG_nn'
Cuts at: 3081 4181 4493 3081
Size: 1100 312 5840
Fragments arranged by size:
5840 1100 312
DraI TTT'AAA
Cuts at: 1565 2261 4249 1565
Size: 696 1988 4568
Fragments arranged by size:
4568 1988 696
DraIII CAC_nnn'GTG
Cuts at: 3098 3098
Size: 7252
DrdI GACnn_nn'nnGTC
Cuts at: 4956 5816 4956
Size: 860 6392
EaeI y'GGCC_r
Cuts at: 4835 5009 5400 5427 6774 4835
Size: 174 391 27 1347 5313
Fragments arranged by size:
5313 1347 391 174 27
EagI C'GGCC_G
Cuts at: 4835 4835
Size: 7252
EarI CTCTTCn'nnn_
Cuts at: 179 296 1355 1643 1665 1934 2333 2978 5273
Size: 117 1059 288 22 269 399 645 2295
Cuts at: 5273 5483 179
Size: 210 1948
Fragments arranged by size:
2295 1948 1059 645 399 288 269 210
117 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 5458 5770 5916 7062 1969
Size: 3489 312 146 1146 2159
Fragments arranged by size:
3489 2159 1146 312 146
EcoICRI GAG'CTC
Cuts at: 4262 4262
Size: 7252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 904 1838 1940 2156 2705 3446 3668 3732
Size: 158 934 102 216 549 741 222 64
Cuts at: 3732 4106 5075 5507 6256 6524 746
Size: 374 969 432 749 268 1474
Fragments arranged by size:
1474 969 934 749 741 549 432 374
268 222 216 158 102 64
EcoNI CCTnn'n_nnAGG
Cuts at: 3938 3938
Size: 7252
EcoO109I rG'GnC_Cy
Cuts at: 3004 4389 3004
Size: 1385 5867
EcoRI G'AATT_C
Cuts at: 709 4254 709
Size: 3545 3707
EcoRV GAT'ATC
Cuts at: 1573 1573
Size: 7252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5269 5269
Size: 7252
FspI TGC'GCA
Cuts at: 5031 5031
Size: 7252
HaeII r_GCGC'y
Cuts at: 4684 4932 5956 6935 4684
Size: 248 1024 979 5001
Fragments arranged by size:
5001 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1315 1347 2038 2070 2920 2952 3385 3417 3424
Size: 32 691 32 850 32 433 32 7
Cuts at: 3424 3456 5092 5122 5124 5154 1315
Size: 32 1636 30 2 30 3413
Fragments arranged by size:
3413 1636 850 691 433 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 1893 2370 2661 4289 1893
Size: 477 291 1628 4856
Fragments arranged by size:
4856 1628 477 291
HindIII A'AGCT_T
Cuts at: 1061 2505 1061
Size: 1444 5808
HpaI GTT'AAC
Cuts at: 1893 1893
Size: 7252
KasI G'GCGC_C
Cuts at: 4680 4928 4680
Size: 248 7004
KpnI G_GTAC'C
Cuts at: 163 4270 163
Size: 4107 3145
MfeI C'AATT_G
Cuts at: 479 1300 2094 479
Size: 821 794 5637
Fragments arranged by size:
5637 821 794
MluI A'CGCG_T
Cuts at: 6685 6685
Size: 7252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1600 2697 5923 6107 1600
Size: 1097 3226 184 2745
Fragments arranged by size:
3226 2745 1097 184
MscI TGG'CCA
Cuts at: 5011 5011
Size: 7252
MslI CAynn'nnrTG
Cuts at: 718 1512 1901 2645 3227 5239 5366 718
Size: 794 389 744 582 2012 127 2604
Fragments arranged by size:
2604 2012 794 744 582 389 127
MspA1I CmG'CkG
Cuts at: 534 681 1494 2386 3463 4361 4675 5035 6050
Size: 147 813 892 1077 898 314 360 1015
Cuts at: 6050 6295 534
Size: 245 1491
Fragments arranged by size:
1491 1077 1015 898 892 813 360 314
245 147
NaeI GCC'GGC
Cuts at: 5431 5431
Size: 7252
NarI GG'CG_CC
Cuts at: 4681 4929 4681
Size: 248 7004
NgoMIV G'CCGG_C
Cuts at: 5429 5429
Size: 7252
NruI TCG'CGA
Cuts at: 5613 5613
Size: 7252
NsiI A_TGCA'T
Cuts at: 3543 3543
Size: 7252
NspI r_CATG'y
Cuts at: 881 1404 4303 4803 5712 881
Size: 523 2899 500 909 2421
Fragments arranged by size:
2899 2421 909 523 500
PasI CC'CwG_GG
Cuts at: 1550 1550
Size: 7252
PciI A'CATG_T
Cuts at: 877 1400 5708 877
Size: 523 4308 2421
Fragments arranged by size:
4308 2421 523
PflMI CCAn_nnn'nTGG
Cuts at: 1147 1383 3951 3997 1147
Size: 236 2568 46 4402
Fragments arranged by size:
4402 2568 236 46
PfoI T'CCnGG_A
Cuts at: 3684 3684
Size: 7252
PmeI GTTT'AAAC
Cuts at: 4249 4249
Size: 7252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2298 6391 6423 2266
Size: 32 4093 32 3095
Fragments arranged by size:
4093 3095 32 32
PsiI TTA'TAA
Cuts at: 2437 4153 2437
Size: 1716 5536
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 7220
PstI C_TGCA'G
Cuts at: 3084 3330 4112 4297 4982 3084
Size: 246 782 185 685 5354
Fragments arranged by size:
5354 782 685 246 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7252
PvuII CAG'CTG
Cuts at: 534 681 2386 4675 5035 534
Size: 147 1705 2289 360 2751
Fragments arranged by size:
2751 2289 1705 360 147
RsrII CG'GwC_CG
Cuts at: 5445 5445
Size: 7252
SacI G_AGCT'C
Cuts at: 4264 4264
Size: 7252
SalI G'TCGA_C
Cuts at: 4287 4287
Size: 7252
SapI GCTCTTCn'nnn_
Cuts at: 296 2333 2978 5273 5483 296
Size: 2037 645 2295 210 2065
Fragments arranged by size:
2295 2065 2037 645 210
SbfI CC_TGCA'GG
Cuts at: 4297 4297
Size: 7252
ScaI AGT'ACT
Cuts at: 731 1740 3725 6769 731
Size: 1009 1985 3044 1214
Fragments arranged by size:
3044 1985 1214 1009
SfcI C'TryA_G
Cuts at: 33 1007 1294 1745 3080 3326 4108 4143 4293
Size: 974 287 451 1335 246 782 35 150
Cuts at: 4293 4978 5973 6164 33
Size: 685 995 191 1121
Fragments arranged by size:
1335 1121 995 974 782 685 451 287
246 191 150 35
SfoI GGC'GCC
Cuts at: 4682 4930 4682
Size: 248 7004
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7252
SmaI CCC'GGG
Cuts at: 4272 4272
Size: 7252
SmlI C'TyrA_G
Cuts at: 574 733 1095 1195 1499 1597 4405 5320 5814
Size: 159 362 100 304 98 2808 915 494
Cuts at: 5814 6076 574
Size: 262 1750
Fragments arranged by size:
2808 1750 915 494 362 304 262 159
100 98
SnaBI TAC'GTA
Cuts at: 4242 4242
Size: 7252
SpeI A'CTAG_T
Cuts at: 67 3625 67
Size: 3558 3694
SphI G_CATG'C
Cuts at: 4303 4803 4303
Size: 500 6752
SspI AAT'ATT
Cuts at: 620 849 4158 620
Size: 229 3309 3714
Fragments arranged by size:
3714 3309 229
StuI AGG'CCT
Cuts at: 3798 3798
Size: 7252
StyI C'CwwG_G
Cuts at: 266 1417 4384 4738 266
Size: 1151 2967 354 2780
Fragments arranged by size:
2967 2780 1151 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1207 1708 3329 4833 4966 5425 5636 1207
Size: 501 1621 1504 133 459 211 2823
Fragments arranged by size:
2823 1621 1504 501 459 211 133
TatI w'GTAC_w
Cuts at: 729 1629 1738 1797 2812 3340 3723 6767 729
Size: 900 109 59 1015 528 383 3044 1214
Fragments arranged by size:
3044 1214 1015 900 528 383 109 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2711 2743 4160 4192 6769 6801 2711
Size: 32 1417 32 2577 32 3162
Fragments arranged by size:
3162 2577 1417 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5047 5047
Size: 7252
XbaI T'CTAG_A
Cuts at: 3859 4281 3859
Size: 422 6830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4745 4745
Size: 7252
XmaI C'CCGG_G
Cuts at: 4270 4270
Size: 7252
XmnI GAAnn'nnTTC
Cuts at: 233 305 887 233
Size: 72 582 6598
Fragments arranged by size:
6598 582 72
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI FseI
FspAI NcoI NdeI NheI NotI PacI PmlI PpuMI
PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI