Restriction map for
pF1KSDA1227
(Circular) (Six-base) MAPSORT of: pF1KSDA1227.seq Check: 2265 from: 1 to: 6335
pF1KSDA1227 6335 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3373 3373
Size: 6335
Acc65I G'GTAC_C
Cuts at: 3351 3351
Size: 6335
AclI AA'CG_TT
Cuts at: 6175 6175
Size: 6335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2431 2660 2804 2999 4160 4592 5339 5607 2431
Size: 229 144 195 1161 432 747 268 3159
Fragments arranged by size:
3159 1161 747 432 268 229 195 144
AflII C'TTAA_G
Cuts at: 1780 1780
Size: 6335
AflIII A'CryG_T
Cuts at: 508 693 1823 2660 2945 3002 3687 4791 5768
Size: 185 1130 837 285 57 685 1104 977
Cuts at: 5768 508
Size: 1075
Fragments arranged by size:
1130 1104 1075 977 837 685 285 185
57
AgeI A'CCGG_T
Cuts at: 4723 4723
Size: 6335
AhdI GACnn_n'nnGTC
Cuts at: 1904 1904
Size: 6335
AleI CACnn'nnGTG
Cuts at: 2576 2576
Size: 6335
AlwNI CAG_nnn'CTG
Cuts at: 1492 1705 2495 3120 3640 5207 1492
Size: 213 790 625 520 1567 2620
Fragments arranged by size:
2620 1567 790 625 520 213
ApaLI G'TGCA_C
Cuts at: 5105 5105
Size: 6335
ApoI r'AATT_y
Cuts at: 1723 1759 1975 3339 5688 5944 1723
Size: 36 216 1364 2349 256 2114
Fragments arranged by size:
2349 2114 1364 256 216 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 6335
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
AvaI C'yCGr_G
Cuts at: 2117 2404 2458 2797 3355 2117
Size: 287 54 339 558 5097
Fragments arranged by size:
5097 558 339 287 54
BamHI G'GATC_C
Cuts at: 3360 3360
Size: 6335
BanI G'GyrC_C
Cuts at: 2501 2535 2793 3351 3765 4013 4048 2501
Size: 34 258 558 414 248 35 4788
Fragments arranged by size:
4788 558 414 258 248 35 34
BanII G_rGCy'C
Cuts at: 255 1424 2621 3349 4379 255
Size: 1169 1197 728 1030 2211
Fragments arranged by size:
2211 1197 1169 1030 728
BbeI G_GCGC'C
Cuts at: 3769 4017 3769
Size: 248 6087
BbsI GAAGACnn'nnnn_
Cuts at: 1798 2707 2729 1798
Size: 909 22 5404
Fragments arranged by size:
5404 909 22
BciVI GTATCCnnnnn_n'
Cuts at: 4233 4994 6335 4233
Size: 761 1341 4233
Fragments arranged by size:
4233 1341 761
BclI T'GATC_A
Cuts at: 2030 3855 2030
Size: 1825 4510
BfrBI ATG'CAT
Cuts at: 2199 2199
Size: 6335
BglI GCCn_nnn'nGGC
Cuts at: 3140 3140
Size: 6335
BglII A'GATC_T
Cuts at: 1784 2113 3850 5887 1784
Size: 329 1737 2037 2232
Fragments arranged by size:
2232 2037 1737 329
BlpI GC'TnA_GC
Cuts at: 2312 3447 2312
Size: 1135 5200
Bme1580I G_kGCm'C
Cuts at: 183 3052 3188 3960 4053 5109 183
Size: 2869 136 772 93 1056 1409
Fragments arranged by size:
2869 1409 1056 772 136 93
BmrI ACTGGGnnnn_n'
Cuts at: 3547 3628 3963 6107 3547
Size: 81 335 2144 3775
Fragments arranged by size:
3775 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1683 4702 1683
Size: 3019 3316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2917 3003 3142 3146 2917
Size: 86 139 4 6106
Fragments arranged by size:
6106 139 86 4
Bpu10I CC'TnA_GC
Cuts at: 2615 2615
Size: 6335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 805 1307 1653 2130 3511 4390 4882 5180
Size: 329 502 346 477 1381 879 492 298
Cuts at: 5180 476
Size: 1631
Fragments arranged by size:
1631 1381 879 502 492 477 346 329
298
BsaI GGTCTCn'nnnn_
Cuts at: 230 939 1511 1640 1740 6039 230
Size: 709 572 129 100 4299 526
Fragments arranged by size:
4299 709 572 526 129 100
BsaAI yAC'GTr
Cuts at: 2946 3327 3688 2946
Size: 381 361 5593
Fragments arranged by size:
5593 381 361
BsaBI GATnn'nnATC
Cuts at: 6 3849 5886 6
Size: 3843 2037 455
Fragments arranged by size:
3843 2037 455
BsaHI Gr'CG_yC
Cuts at: 3766 4014 4716 6212 3766
Size: 248 702 1496 3889
Fragments arranged by size:
3889 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3524 4045 4723 4997 5144 5555 3524
Size: 521 678 274 147 411 4304
Fragments arranged by size:
4304 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2902 2932 3051 3081 2902
Size: 30 119 30 6156
Fragments arranged by size:
6156 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 1132 2246 2779 2791 205
Size: 927 1114 533 12 3749
Fragments arranged by size:
3749 1114 927 533 12
BseYI C'CCAG_C
Cuts at: 1423 1566 2609 3760 4518 5095 5591 1423
Size: 143 1043 1151 758 577 496 2167
Fragments arranged by size:
2167 1151 1043 758 577 496 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 140
Size: 6335
BsiEI CG_ry'CG
Cuts at: 104 2369 3877 3923 5131 104
Size: 2265 1508 46 1208 1308
Fragments arranged by size:
2265 1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 255 1631 2923 3349 4127 4317 5109 255
Size: 1376 1292 426 778 190 792 1481
Fragments arranged by size:
1481 1376 1292 792 778 426 190
BsmI GAATG_Cn'
Cuts at: 789 789
Size: 6335
BsmBI CGTCTCn'nnnn_
Cuts at: 202 202
Size: 6335
Bsp1286I G_dGCh'C
Cuts at: 183 255 1424 1631 2621 2923 3052 3188 3349
Size: 72 1169 207 990 302 129 136 161
Cuts at: 3349 3960 4053 4127 4317 4379 5109 183
Size: 611 93 74 190 62 730 1409
Fragments arranged by size:
1409 1169 990 730 611 302 207 190
161 136 129 93 74 72 62
BspEI T'CCGG_A
Cuts at: 5555 5555
Size: 6335
BspHI T'CATG_A
Cuts at: 5511 5511
Size: 6335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1792 2461 3385 3599 3901 4282 1792
Size: 669 924 214 302 381 3845
Fragments arranged by size:
3845 924 669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2349 4627 4681 6165 6330 50
Size: 2299 2278 54 1484 165 55
Fragments arranged by size:
2299 2278 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 662 1773 4247 662
Size: 1111 2474 2750
Fragments arranged by size:
2750 2474 1111
BsrFI r'CCGG_y
Cuts at: 4333 4514 4723 4333
Size: 181 209 5945
Fragments arranged by size:
5945 209 181
BssSI C'ACGA_G
Cuts at: 2344 2422 4606 4964 2344
Size: 78 2184 358 3715
Fragments arranged by size:
3715 2184 358 78
BstAPI GCAn_nnn'nTGC
Cuts at: 1775 3712 1775
Size: 1937 4398
BstBI TT'CG_AA
Cuts at: 110 4696 110
Size: 4586 1749
BstYI r'GATC_y
Cuts at: 1 796 1087 1784 2113 3110 3360 3827 3850
Size: 795 291 697 329 997 250 467 23
Cuts at: 3850 4185 4431 5443 5529 5541 5887 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 997 795 697 467 449 346 335
329 291 250 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1345 2389 1345
Size: 1044 5291
BtgI C'CryG_G
Cuts at: 125 949 125
Size: 824 5511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1324 4285 4466 4629 1324
Size: 2961 181 163 3030
Fragments arranged by size:
3030 2961 181 163
BtsI GCAGTG_nn'
Cuts at: 848 3100 3578 848
Size: 2252 478 3605
Fragments arranged by size:
3605 2252 478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 944 979 944
Size: 35 6300
DraI TTT'AAA
Cuts at: 377 1283 1764 3243 3334 377
Size: 906 481 1479 91 3378
Fragments arranged by size:
3378 1479 906 481 91
DrdI GACnn_nn'nnGTC
Cuts at: 2984 4041 4899 2984
Size: 1057 858 4420
Fragments arranged by size:
4420 1057 858
EaeI y'GGCC_r
Cuts at: 748 952 2366 3920 4094 4485 4512 5857 748
Size: 204 1414 1554 174 391 27 1345 1226
Fragments arranged by size:
1554 1414 1345 1226 391 204 174 27
EagI C'GGCC_G
Cuts at: 2366 3920 2366
Size: 1554 4781
EarI CTCTTCn'nnn_
Cuts at: 406 937 2325 2783 4358 4568 406
Size: 531 1388 458 1575 210 2173
Fragments arranged by size:
2173 1575 1388 531 458 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4543 4853 4999 6145 4543
Size: 310 146 1146 4733
Fragments arranged by size:
4733 1146 310 146
EcoICRI GAG'CTC
Cuts at: 253 3347 253
Size: 3094 3241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2431 2660 2804 2917 2999 3003 3142 3146 4160
Size: 229 144 113 82 4 139 4 1014
Cuts at: 4160 4592 5339 5607 2431
Size: 432 747 268 3159
Fragments arranged by size:
3159 1014 747 432 268 229 144 139
113 82 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 187 187
Size: 6335
EcoO109I rG'GnC_Cy
Cuts at: 1349 2025 2600 2827 3474 1349
Size: 676 575 227 647 4210
Fragments arranged by size:
4210 676 647 575 227
EcoRI G'AATT_C
Cuts at: 3339 3339
Size: 6335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 659 1083 2124 4354 659
Size: 424 1041 2230 2640
Fragments arranged by size:
2640 2230 1041 424
FspI TGC'GCA
Cuts at: 1408 2470 4116 1408
Size: 1062 1646 3627
Fragments arranged by size:
3627 1646 1062
FspAI rTGC'GCAy
Cuts at: 1408 2470 1408
Size: 1062 5273
HaeII r_GCGC'y
Cuts at: 3769 4017 5039 6018 3769
Size: 248 1022 979 4086
Fragments arranged by size:
4086 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 978 1010 1189 1221 1618 1650 1890 1922 3102
Size: 32 179 32 397 32 240 32 1180
Cuts at: 3102 3134 4177 4207 4209 4239 978
Size: 32 1043 30 2 30 3074
Fragments arranged by size:
3074 1180 1043 397 240 179 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1821 2688 3374 1370
Size: 451 867 686 4331
Fragments arranged by size:
4331 867 686 451
HindIII A'AGCT_T
Cuts at: 2957 2957
Size: 6335
HpaI GTT'AAC
Cuts at: 1821 2688 1821
Size: 867 5468
KasI G'GCGC_C
Cuts at: 3765 4013 3765
Size: 248 6087
KpnI G_GTAC'C
Cuts at: 3355 3355
Size: 6335
MluI A'CGCG_T
Cuts at: 5768 5768
Size: 6335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 529 1418 1715 5006 5190 213
Size: 316 889 297 3291 184 1358
Fragments arranged by size:
3291 1358 889 316 297 184
MscI TGG'CCA
Cuts at: 4096 4096
Size: 6335
MslI CAynn'nnrTG
Cuts at: 1814 2576 4324 4451 1814
Size: 762 1748 127 3698
Fragments arranged by size:
3698 1748 762 127
MspA1I CmG'CkG
Cuts at: 210 1434 1492 2895 3446 3760 4120 5133 5378
Size: 1224 58 1403 551 314 360 1013 245
Cuts at: 5378 210
Size: 1167
Fragments arranged by size:
1403 1224 1167 1013 551 360 314 245
58
NaeI GCC'GGC
Cuts at: 4516 4516
Size: 6335
NarI GG'CG_CC
Cuts at: 3766 4014 3766
Size: 248 6087
NcoI C'CATG_G
Cuts at: 125 125
Size: 6335
NgoMIV G'CCGG_C
Cuts at: 4514 4514
Size: 6335
NsiI A_TGCA'T
Cuts at: 2201 2201
Size: 6335
NspI r_CATG'y
Cuts at: 697 1827 1960 2254 2469 3006 3388 3888 4795
Size: 1130 133 294 215 537 382 500 907
Cuts at: 4795 697
Size: 2237
Fragments arranged by size:
2237 1130 907 537 500 382 294 215
133
PasI CC'CwG_GG
Cuts at: 1212 1212
Size: 6335
PciI A'CATG_T
Cuts at: 693 1823 3002 4791 693
Size: 1130 1179 1789 2237
Fragments arranged by size:
2237 1789 1179 1130
PflMI CCAn_nnn'nTGG
Cuts at: 1084 2849 3120 1084
Size: 1765 271 4299
Fragments arranged by size:
4299 1765 271
PfoI T'CCnGG_A
Cuts at: 2202 2202
Size: 6335
PmeI GTTT'AAAC
Cuts at: 3334 3334
Size: 6335
PmlI CAC'GTG
Cuts at: 2946 2946
Size: 6335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 5474
Size: 32 6303
PpuMI rG'GwC_Cy
Cuts at: 1349 2827 1349
Size: 1478 4857
PshAI GACnn'nnGTC
Cuts at: 1862 2729 1862
Size: 867 5468
PspXI vC'TCGA_Gb
Cuts at: 2458 2458
Size: 6335
PstI C_TGCA'G
Cuts at: 2386 3382 4067 2386
Size: 996 685 4654
Fragments arranged by size:
4654 996 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6335
PvuII CAG'CTG
Cuts at: 210 1492 2895 3760 4120 210
Size: 1282 1403 865 360 2425
Fragments arranged by size:
2425 1403 1282 865 360
RsrII CG'GwC_CG
Cuts at: 1590 4530 1590
Size: 2940 3395
SacI G_AGCT'C
Cuts at: 255 3349 255
Size: 3094 3241
SalI G'TCGA_C
Cuts at: 3372 3372
Size: 6335
SapI GCTCTTCn'nnn_
Cuts at: 406 4358 4568 406
Size: 3952 210 2173
Fragments arranged by size:
3952 2173 210
SbfI CC_TGCA'GG
Cuts at: 3382 3382
Size: 6335
ScaI AGT'ACT
Cuts at: 5852 5852
Size: 6335
SexAI A'CCwGG_T
Cuts at: 891 2504 891
Size: 1613 4722
SfcI C'TryA_G
Cuts at: 33 444 2382 3378 4063 5056 5247 33
Size: 411 1938 996 685 993 191 1121
Fragments arranged by size:
1938 1121 996 993 685 411 191
SfoI GGC'GCC
Cuts at: 3767 4015 3767
Size: 248 6087
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
SmaI CCC'GGG
Cuts at: 3357 3357
Size: 6335
SmlI C'TyrA_G
Cuts at: 491 784 1322 1668 1780 2109 2117 2458 3490
Size: 293 538 346 112 329 8 341 1032
Cuts at: 3490 4405 4897 5159 491
Size: 915 492 262 1667
Fragments arranged by size:
1667 1032 915 538 492 346 341 329
293 262 112 8
SnaBI TAC'GTA
Cuts at: 3327 3327
Size: 6335
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6335
SphI G_CATG'C
Cuts at: 2254 2469 3388 3888 2254
Size: 215 919 500 4701
Fragments arranged by size:
4701 919 500 215
StuI AGG'CCT
Cuts at: 1320 3143 1320
Size: 1823 4512
StyI C'CwwG_G
Cuts at: 125 2217 3469 3823 125
Size: 2092 1252 354 2637
Fragments arranged by size:
2637 2092 1252 354
SwaI ATTT'AAAT
Cuts at: 1764 1764
Size: 6335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 1607 2553 3918 4051 4510 4719 1497
Size: 110 946 1365 133 459 209 3113
Fragments arranged by size:
3113 1365 946 459 209 133 110
TatI w'GTAC_w
Cuts at: 1049 2140 5850 1049
Size: 1091 3710 1534
Fragments arranged by size:
3710 1534 1091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2902 2934 3051 3083 5852 5884 2902
Size: 32 117 32 2769 32 3353
Fragments arranged by size:
3353 2769 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4132 4132
Size: 6335
XbaI T'CTAG_A
Cuts at: 927 3366 927
Size: 2439 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3830 3830
Size: 6335
XhoI C'TCGA_G
Cuts at: 2117 2458 2117
Size: 341 5994
XmaI C'CCGG_G
Cuts at: 3355 3355
Size: 6335
XmnI GAAnn'nnTTC
Cuts at: 287 716 2875 287
Size: 429 2159 3747
Fragments arranged by size:
3747 2159 429
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsiWI BsrGI BssHII
BstEII BstXI BstZ17I ClaI DraIII EcoRV FseI MfeI
NdeI NheI NotI NruI PacI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SrfI SspI ZraI