Restriction map for
pF1KSDA1229
(Circular) (Six-base) MAPSORT of: pF1KSDA1229.seq Check: 9448 from: 1 to: 5047
pF1KSDA1229 5047 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2083 2083
Size: 5047
Acc65I G'GTAC_C
Cuts at: 2061 2061
Size: 5047
AclI AA'CG_TT
Cuts at: 4887 4887
Size: 5047
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1093 1531 1618 1729 1919 2870 3302 4051
Size: 831 438 87 111 190 951 432 749
Cuts at: 4051 4319 262
Size: 268 990
Fragments arranged by size:
990 951 831 749 438 432 268 190
111 87
AflII C'TTAA_G
Cuts at: 286 565 1798 1813 286
Size: 279 1233 15 3520
Fragments arranged by size:
3520 1233 279 15
AflIII A'CryG_T
Cuts at: 2397 3503 4480 2397
Size: 1106 977 2964
Fragments arranged by size:
2964 1106 977
AgeI A'CCGG_T
Cuts at: 3435 3435
Size: 5047
AlwNI CAG_nnn'CTG
Cuts at: 2350 3919 2350
Size: 1569 3478
ApaLI G'TGCA_C
Cuts at: 3817 3817
Size: 5047
ApoI r'AATT_y
Cuts at: 390 701 1098 1112 1428 2049 4400 4656 390
Size: 311 397 14 316 621 2351 256 781
Fragments arranged by size:
2351 781 621 397 316 311 256 14
AseI AT'TA_AT
Cuts at: 20 359 421 1055 20
Size: 339 62 634 4012
Fragments arranged by size:
4012 634 339 62
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5047
AvaI C'yCGr_G
Cuts at: 2065 2065
Size: 5047
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1372 1405 1372
Size: 33 5014
BamHI G'GATC_C
Cuts at: 2070 2070
Size: 5047
BanI G'GyrC_C
Cuts at: 2061 2475 2723 2758 2061
Size: 414 248 35 4350
Fragments arranged by size:
4350 414 248 35
BanII G_rGCy'C
Cuts at: 2059 3089 2059
Size: 1030 4017
BbeI G_GCGC'C
Cuts at: 2479 2727 2479
Size: 248 4799
BbsI GAAGACnn'nnnn_
Cuts at: 1091 1890 1091
Size: 799 4248
BciVI GTATCCnnnnn_n'
Cuts at: 2943 3706 5047 2943
Size: 763 1341 2943
Fragments arranged by size:
2943 1341 763
BclI T'GATC_A
Cuts at: 2565 2565
Size: 5047
BglII A'GATC_T
Cuts at: 1068 1953 2560 4599 1068
Size: 885 607 2039 1516
Fragments arranged by size:
2039 1516 885 607
BlpI GC'TnA_GC
Cuts at: 2157 2157
Size: 5047
Bme1580I G_kGCm'C
Cuts at: 2670 2763 3821 2670
Size: 93 1058 3896
Fragments arranged by size:
3896 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2257 2338 2673 4819 2257
Size: 81 335 2146 2485
Fragments arranged by size:
2485 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 5047
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 1786 2221 3100 3594 3892 1183
Size: 603 435 879 494 298 2338
Fragments arranged by size:
2338 879 603 494 435 298
BsaI GGTCTCn'nnnn_
Cuts at: 4751 4751
Size: 5047
BsaAI yAC'GTr
Cuts at: 2037 2398 2037
Size: 361 4686
BsaBI GATnn'nnATC
Cuts at: 6 1652 2559 4598 6
Size: 1646 907 2039 455
Fragments arranged by size:
2039 1646 907 455
BsaHI Gr'CG_yC
Cuts at: 2476 2724 3428 4924 2476
Size: 248 704 1496 2599
Fragments arranged by size:
2599 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2234 2755 3435 3709 3856 4267 2234
Size: 521 680 274 147 411 3014
Fragments arranged by size:
3014 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2470 3228 3807 4303 2470
Size: 758 579 496 3214
Fragments arranged by size:
3214 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1625 1625
Size: 5047
BsiEI CG_ry'CG
Cuts at: 104 2587 2633 3843 104
Size: 2483 46 1210 1308
Fragments arranged by size:
2483 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2059 2837 3027 3821 2059
Size: 778 190 794 3285
Fragments arranged by size:
3285 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 2059 2670 2763 2837 3027 3089 3821 2059
Size: 611 93 74 190 62 732 3285
Fragments arranged by size:
3285 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4267 4267
Size: 5047
BspHI T'CATG_A
Cuts at: 4223 4223
Size: 5047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 788 2095 2309 2611 2992 788
Size: 1307 214 302 381 2843
Fragments arranged by size:
2843 1307 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3337 3391 4877 5042 50
Size: 3287 54 1486 165 55
Fragments arranged by size:
3287 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2957 2957
Size: 5047
BsrFI r'CCGG_y
Cuts at: 3043 3224 3435 3043
Size: 181 211 4655
Fragments arranged by size:
4655 211 181
BssSI C'ACGA_G
Cuts at: 1564 3316 3676 1564
Size: 1752 360 2935
Fragments arranged by size:
2935 1752 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2422 2422
Size: 5047
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5047
BstXI CCAn_nnnn'nTGG
Cuts at: 1648 1860 1648
Size: 212 4835
BstYI r'GATC_y
Cuts at: 1 695 1068 1953 2070 2537 2560 2895 3141
Size: 694 373 885 117 467 23 335 246
Cuts at: 3141 4155 4241 4253 4599 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 885 694 467 449 373 346 335
246 117 86 23 12
BtgI C'CryG_G
Cuts at: 125 208 1089 125
Size: 83 881 4083
Fragments arranged by size:
4083 881 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2995 3176 3339 2995
Size: 181 163 4703
Fragments arranged by size:
4703 181 163
BtsI GCAGTG_nn'
Cuts at: 1591 2288 1591
Size: 697 4350
DraI TTT'AAA
Cuts at: 851 1821 2044 851
Size: 970 223 3854
Fragments arranged by size:
3854 970 223
DrdI GACnn_nn'nnGTC
Cuts at: 2751 3611 2751
Size: 860 4187
EaeI y'GGCC_r
Cuts at: 1850 2630 2804 3195 3222 4569 1850
Size: 780 174 391 27 1347 2328
Fragments arranged by size:
2328 1347 780 391 174 27
EagI C'GGCC_G
Cuts at: 2630 2630
Size: 5047
EarI CTCTTCn'nnn_
Cuts at: 1679 1979 3068 3278 1679
Size: 300 1089 210 3448
Fragments arranged by size:
3448 1089 300 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1564 3253 3565 3711 4857 1564
Size: 1689 312 146 1146 1754
Fragments arranged by size:
1754 1689 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2057 2057
Size: 5047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1093 1531 1618 1729 1919 2870 3302 4051
Size: 831 438 87 111 190 951 432 749
Cuts at: 4051 4319 262
Size: 268 990
Fragments arranged by size:
990 951 831 749 438 432 268 190
111 87
EcoNI CCTnn'n_nnAGG
Cuts at: 244 244
Size: 5047
EcoO109I rG'GnC_Cy
Cuts at: 2184 2184
Size: 5047
EcoRI G'AATT_C
Cuts at: 2049 2049
Size: 5047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 301 1132 1813 3064 301
Size: 831 681 1251 2284
Fragments arranged by size:
2284 1251 831 681
FspI TGC'GCA
Cuts at: 2826 2826
Size: 5047
HaeII r_GCGC'y
Cuts at: 2479 2727 3751 4730 2479
Size: 248 1024 979 2796
Fragments arranged by size:
2796 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 528 680 712 1488 1520 1900 1932 2887
Size: 32 152 32 776 32 380 32 955
Cuts at: 2887 2917 2919 2949 496
Size: 30 2 30 2594
Fragments arranged by size:
2594 955 776 380 152 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2084 2084
Size: 5047
HindIII A'AGCT_T
Cuts at: 910 1180 1672 910
Size: 270 492 4285
Fragments arranged by size:
4285 492 270
KasI G'GCGC_C
Cuts at: 2475 2723 2475
Size: 248 4799
KpnI G_GTAC'C
Cuts at: 2065 2065
Size: 5047
MfeI C'AATT_G
Cuts at: 1133 1133
Size: 5047
MluI A'CGCG_T
Cuts at: 4480 4480
Size: 5047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3718 3902 3718
Size: 184 4863
MscI TGG'CCA
Cuts at: 1852 2806 1852
Size: 954 4093
MslI CAynn'nnrTG
Cuts at: 1145 3034 3161 1145
Size: 1889 127 3031
Fragments arranged by size:
3031 1889 127
MspA1I CmG'CkG
Cuts at: 236 1402 1707 2156 2470 2830 3845 4090 236
Size: 1166 305 449 314 360 1015 245 1193
Fragments arranged by size:
1193 1166 1015 449 360 314 305 245
NaeI GCC'GGC
Cuts at: 3226 3226
Size: 5047
NarI GG'CG_CC
Cuts at: 2476 2724 2476
Size: 248 4799
NcoI C'CATG_G
Cuts at: 125 1089 125
Size: 964 4083
NgoMIV G'CCGG_C
Cuts at: 3224 3224
Size: 5047
NruI TCG'CGA
Cuts at: 3408 3408
Size: 5047
NspI r_CATG'y
Cuts at: 522 2098 2598 3507 522
Size: 1576 500 909 2062
Fragments arranged by size:
2062 1576 909 500
PciI A'CATG_T
Cuts at: 3503 3503
Size: 5047
PflMI CCAn_nnn'nTGG
Cuts at: 974 974
Size: 5047
PmeI GTTT'AAAC
Cuts at: 2044 2044
Size: 5047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4186 4218 4186
Size: 32 5015
PstI C_TGCA'G
Cuts at: 1586 1836 2092 2777 1586
Size: 250 256 685 3856
Fragments arranged by size:
3856 685 256 250
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5047
PvuII CAG'CTG
Cuts at: 236 1402 1707 2470 2830 236
Size: 1166 305 763 360 2453
Fragments arranged by size:
2453 1166 763 360 305
RsrII CG'GwC_CG
Cuts at: 3240 3240
Size: 5047
SacI G_AGCT'C
Cuts at: 2059 2059
Size: 5047
SalI G'TCGA_C
Cuts at: 2082 2082
Size: 5047
SapI GCTCTTCn'nnn_
Cuts at: 1979 3068 3278 1979
Size: 1089 210 3748
Fragments arranged by size:
3748 1089 210
SbfI CC_TGCA'GG
Cuts at: 2092 2092
Size: 5047
ScaI AGT'ACT
Cuts at: 4564 4564
Size: 5047
SfcI C'TryA_G
Cuts at: 33 314 691 809 842 1582 1832 2088 2773
Size: 281 377 118 33 740 250 256 685
Cuts at: 2773 3768 3959 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 740 685 377 281 256 250
191 118 33
SfoI GGC'GCC
Cuts at: 2477 2725 2477
Size: 248 4799
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5047
SmaI CCC'GGG
Cuts at: 2067 2067
Size: 5047
SmlI C'TyrA_G
Cuts at: 286 565 1162 1765 1798 1813 2200 3115 3609
Size: 279 597 603 33 15 387 915 494
Cuts at: 3609 3871 286
Size: 262 1462
Fragments arranged by size:
1462 915 603 597 494 387 279 262
33 15
SnaBI TAC'GTA
Cuts at: 2037 2037
Size: 5047
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5047
SphI G_CATG'C
Cuts at: 2098 2598 2098
Size: 500 4547
SspI AAT'ATT
Cuts at: 419 1949 419
Size: 1530 3517
StyI C'CwwG_G
Cuts at: 125 1089 2179 2533 125
Size: 964 1090 354 2639
Fragments arranged by size:
2639 1090 964 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2628 2761 3220 3431 2628
Size: 133 459 211 4244
Fragments arranged by size:
4244 459 211 133
TatI w'GTAC_w
Cuts at: 678 1023 4562 678
Size: 345 3539 1163
Fragments arranged by size:
3539 1163 345
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4564 4596 4564
Size: 32 5015
Tth111I GACn'n_nGTC
Cuts at: 2842 2842
Size: 5047
XbaI T'CTAG_A
Cuts at: 1921 2076 1921
Size: 155 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 5047
XmaI C'CCGG_G
Cuts at: 2065 2065
Size: 5047
XmnI GAAnn'nnTTC
Cuts at: 153 470 1098 1112 1721 153
Size: 317 628 14 609 3479
Fragments arranged by size:
3479 628 609 317 14
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI NdeI NheI NotI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI