Restriction map for
pF1KSDA1234
(Circular) (Six-base) MAPSORT of: pF1KSDA1234.seq Check: 5148 from: 1 to: 5296
pF1KSDA1234 5296 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2134
Size: 5296
AccI GT'mk_AC
Cuts at: 2332 2332
Size: 5296
Acc65I G'GTAC_C
Cuts at: 2310 2310
Size: 5296
AclI AA'CG_TT
Cuts at: 5136 5136
Size: 5296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3551 4300 4568 3119
Size: 432 749 268 3847
Fragments arranged by size:
3847 749 432 268
AflIII A'CryG_T
Cuts at: 2646 3752 4729 2646
Size: 1106 977 3213
Fragments arranged by size:
3213 1106 977
AgeI A'CCGG_T
Cuts at: 3684 3684
Size: 5296
AleI CACnn'nnGTG
Cuts at: 2181 2181
Size: 5296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1609 2293 1609
Size: 684 4612
AlwNI CAG_nnn'CTG
Cuts at: 457 2599 4168 457
Size: 2142 1569 1585
Fragments arranged by size:
2142 1585 1569
ApaI G_GGCC'C
Cuts at: 744 1245 744
Size: 501 4795
ApaLI G'TGCA_C
Cuts at: 156 1618 2096 2268 4066 156
Size: 1462 478 172 1798 1386
Fragments arranged by size:
1798 1462 1386 478 172
ApoI r'AATT_y
Cuts at: 925 2298 4649 4905 925
Size: 1373 2351 256 1316
Fragments arranged by size:
2351 1373 1316 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5296
AvaI C'yCGr_G
Cuts at: 834 848 886 2212 2314 834
Size: 14 38 1326 102 3816
Fragments arranged by size:
3816 1326 102 38 14
BamHI G'GATC_C
Cuts at: 2319 2319
Size: 5296
BanI G'GyrC_C
Cuts at: 418 951 1002 1423 2208 2310 2724 2972 3007
Size: 533 51 421 785 102 414 248 35
Cuts at: 3007 418
Size: 2707
Fragments arranged by size:
2707 785 533 421 414 248 102 51
35
BanII G_rGCy'C
Cuts at: 400 744 1245 1300 1555 1683 1974 2054 2168
Size: 344 501 55 255 128 291 80 114
Cuts at: 2168 2308 3338 400
Size: 140 1030 2358
Fragments arranged by size:
2358 1030 501 344 291 255 140 128
114 80 55
BbeI G_GCGC'C
Cuts at: 422 955 2728 2976 422
Size: 533 1773 248 2742
Fragments arranged by size:
2742 1773 533 248
BbvCI CC'TCA_GC
Cuts at: 1670 2138 1670
Size: 468 4828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1755 1789 1755
Size: 34 5262
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3955 5296 3192
Size: 763 1341 3192
Fragments arranged by size:
3192 1341 763
BclI T'GATC_A
Cuts at: 2814 2814
Size: 5296
BfrBI ATG'CAT
Cuts at: 541 541
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 214 214
Size: 5296
BglII A'GATC_T
Cuts at: 2809 4848 2809
Size: 2039 3257
BlpI GC'TnA_GC
Cuts at: 1333 1682 1973 2406 1333
Size: 349 291 433 4223
Fragments arranged by size:
4223 433 349 291
Bme1580I G_kGCm'C
Cuts at: 160 744 757 1227 1245 1519 1622 1841 1958
Size: 584 13 470 18 274 103 219 117
Cuts at: 1958 2100 2213 2272 2919 3012 4070 160
Size: 142 113 59 647 93 1058 1386
Fragments arranged by size:
1386 1058 647 584 470 274 219 142
117 113 103 93 59 18 13
BmrI ACTGGGnnnn_n'
Cuts at: 663 1556 2064 2175 2506 2587 2922 5068 663
Size: 893 508 111 331 81 335 2146 891
Fragments arranged by size:
2146 893 891 508 335 331 111 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 414 985 1385 1821 1938 3661 414
Size: 571 400 436 117 1723 2049
Fragments arranged by size:
2049 1723 571 436 400 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1355 1367 1557 1697 2068 487
Size: 868 12 190 140 371 3715
Fragments arranged by size:
3715 868 371 190 140 12
Bpu10I CC'TnA_GC
Cuts at: 736 1195 1299 1670 2138 736
Size: 459 104 371 468 3894
Fragments arranged by size:
3894 468 459 371 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 3349 3843 4141 2470
Size: 879 494 298 3625
Fragments arranged by size:
3625 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5000 5000
Size: 5296
BsaAI yAC'GTr
Cuts at: 161 618 2286 2647 161
Size: 457 1668 361 2810
Fragments arranged by size:
2810 1668 457 361
BsaBI GATnn'nnATC
Cuts at: 6 2808 4847 6
Size: 2802 2039 455
Fragments arranged by size:
2802 2039 455
BsaHI Gr'CG_yC
Cuts at: 419 952 2725 2973 3677 5173 419
Size: 533 1773 248 704 1496 542
Fragments arranged by size:
1773 1496 704 542 533 248
BsaWI w'CCGG_w
Cuts at: 2483 3004 3684 3958 4105 4516 2483
Size: 521 680 274 147 411 3263
Fragments arranged by size:
3263 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 295 325 995 1025 1447 1477 145
Size: 30 120 30 670 30 422 30 3964
Fragments arranged by size:
3964 670 422 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 195 1003 1056 1327 1330 1662 195
Size: 808 53 271 3 332 3829
Fragments arranged by size:
3829 808 332 271 53 3
BseYI C'CCAG_C
Cuts at: 1444 1840 1854 1878 1996 2719 3477 4056 4552
Size: 396 14 24 118 723 758 579 496
Cuts at: 4552 1444
Size: 2188
Fragments arranged by size:
2188 758 723 579 496 396 118 24
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 539 2015 2106 406
Size: 133 1476 91 3596
Fragments arranged by size:
3596 1476 133 91
BsiEI CG_ry'CG
Cuts at: 104 416 554 627 2836 2882 4092 104
Size: 312 138 73 2209 46 1210 1308
Fragments arranged by size:
2209 1308 1210 312 138 73 46
BsiHKAI G_wGCw'C
Cuts at: 160 400 1196 1622 1683 2100 2272 2308 3086
Size: 240 796 426 61 417 172 36 778
Cuts at: 3086 3276 4070 160
Size: 190 794 1386
Fragments arranged by size:
1386 796 794 778 426 417 240 190
172 61 36
BsiWI C'GTAC_G
Cuts at: 161 161
Size: 5296
Bsp1286I G_dGCh'C
Cuts at: 160 400 744 757 1196 1227 1245 1300 1519
Size: 240 344 13 439 31 18 55 219
Cuts at: 1519 1555 1622 1683 1841 1958 1974 2054 2100
Size: 36 67 61 158 117 16 80 46
Cuts at: 2100 2168 2213 2272 2308 2919 3012 3086 3276
Size: 68 45 59 36 611 93 74 190
Cuts at: 3276 3338 4070 160
Size: 62 732 1386
Fragments arranged by size:
1386 732 611 439 344 240 219 190
158 117 93 80 74 68 67 62
61 59 55 46 45 36 36 31
18 16 13
BspEI T'CCGG_A
Cuts at: 4516 4516
Size: 5296
BspHI T'CATG_A
Cuts at: 896 4472 896
Size: 3576 1720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2134 2344 2558 2860 3241 2134
Size: 210 214 302 381 4189
Fragments arranged by size:
4189 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 1181 3586 3640 5126 5291 50
Size: 1131 2405 54 1486 165 55
Fragments arranged by size:
2405 1486 1131 165 55 54
BsrDI GCAATG_nn'
Cuts at: 995 3206 995
Size: 2211 3085
BsrFI r'CCGG_y
Cuts at: 415 641 3292 3473 3684 415
Size: 226 2651 181 211 2027
Fragments arranged by size:
2651 2027 226 211 181
BsrGI T'GTAC_A
Cuts at: 1937 1937
Size: 5296
BssHII G'CGCG_C
Cuts at: 1046 1046
Size: 5296
BssSI C'ACGA_G
Cuts at: 1695 3565 3925 1695
Size: 1870 360 3066
Fragments arranged by size:
3066 1870 360
BstAPI GCAn_nnn'nTGC
Cuts at: 457 1329 1962 2038 2671 457
Size: 872 633 76 633 3082
Fragments arranged by size:
3082 872 633 633 76
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5296
BstXI CCAn_nnnn'nTGG
Cuts at: 1794 1794
Size: 5296
BstYI r'GATC_y
Cuts at: 1 2319 2786 2809 3144 3390 4404 4490 4502
Size: 2318 467 23 335 246 1014 86 12
Cuts at: 4502 4848 1
Size: 346 449
Fragments arranged by size:
2318 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1054 1054
Size: 5296
BtgI C'CryG_G
Cuts at: 209 659 976 1088 1482 1709 1980 209
Size: 450 317 112 394 227 271 3525
Fragments arranged by size:
3525 450 394 317 271 227 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4952
Fragments arranged by size:
4952 181 163
BtsI GCAGTG_nn'
Cuts at: 1991 2537 1991
Size: 546 4750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 536 571 536
Size: 35 5261
DraI TTT'AAA
Cuts at: 2293 2293
Size: 5296
DraIII CAC_nnn'GTG
Cuts at: 1962 2038 1962
Size: 76 5220
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3860 3000
Size: 860 4436
EaeI y'GGCC_r
Cuts at: 278 413 1215 2237 2879 3053 3444 3471 4818
Size: 135 802 1022 642 174 391 27 1347
Cuts at: 4818 278
Size: 756
Fragments arranged by size:
1347 1022 802 756 642 391 174 135
27
EagI C'GGCC_G
Cuts at: 413 2879 413
Size: 2466 2830
EarI CTCTTCn'nnn_
Cuts at: 361 3317 3527 361
Size: 2956 210 2130
Fragments arranged by size:
2956 2130 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1374 3502 3814 3960 5106 1374
Size: 2128 312 146 1146 1564
Fragments arranged by size:
2128 1564 1146 312 146
EcoICRI GAG'CTC
Cuts at: 398 1681 2306 398
Size: 1283 625 3388
Fragments arranged by size:
3388 1283 625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1355 1367 1557 1697 2068 3119 3551 4300
Size: 868 12 190 140 371 1051 432 749
Cuts at: 4300 4568 487
Size: 268 1215
Fragments arranged by size:
1215 1051 868 749 432 371 268 190
140 12
EcoNI CCTnn'n_nnAGG
Cuts at: 449 449
Size: 5296
EcoO109I rG'GnC_Cy
Cuts at: 185 690 740 1241 1242 1340 1375 1752 2433
Size: 505 50 501 1 98 35 377 681
Cuts at: 2433 185
Size: 3048
Fragments arranged by size:
3048 681 505 501 377 98 50 35
1
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 3313 390
Size: 2923 2373
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5296
HaeII r_GCGC'y
Cuts at: 422 955 2728 2976 4000 4979 422
Size: 533 1773 248 1024 979 739
Fragments arranged by size:
1773 1024 979 739 533 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 817 849 1447 1479 1689 1721 3136
Size: 32 490 32 598 32 210 32 1415
Cuts at: 3136 3166 3168 3198 295
Size: 30 2 30 2393
Fragments arranged by size:
2393 1415 598 490 210 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2333 2333
Size: 5296
HindIII A'AGCT_T
Cuts at: 325 2258 325
Size: 1933 3363
KasI G'GCGC_C
Cuts at: 418 951 2724 2972 418
Size: 533 1773 248 2742
Fragments arranged by size:
2742 1773 533 248
KpnI G_GTAC'C
Cuts at: 2314 2314
Size: 5296
MluI A'CGCG_T
Cuts at: 4729 4729
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 251 464 1789 2054 3967 4151 248
Size: 3 213 1325 265 1913 184 1393
Fragments arranged by size:
1913 1393 1325 265 213 184 3
MscI TGG'CCA
Cuts at: 280 2239 3055 280
Size: 1959 816 2521
Fragments arranged by size:
2521 1959 816
MslI CAynn'nnrTG
Cuts at: 616 901 1251 1792 2181 3283 3410 616
Size: 285 350 541 389 1102 127 2502
Fragments arranged by size:
2502 1102 541 389 350 285 127
MspA1I CmG'CkG
Cuts at: 440 794 870 978 1027 1090 1402 1484 2405
Size: 354 76 108 49 63 312 82 921
Cuts at: 2405 2719 3079 4094 4339 440
Size: 314 360 1015 245 1397
Fragments arranged by size:
1397 1015 921 360 354 314 312 245
108 82 76 63 49
NaeI GCC'GGC
Cuts at: 417 643 3475 417
Size: 226 2832 2238
Fragments arranged by size:
2832 2238 226
NarI GG'CG_CC
Cuts at: 419 952 2725 2973 419
Size: 533 1773 248 2742
Fragments arranged by size:
2742 1773 533 248
NcoI C'CATG_G
Cuts at: 659 1709 1980 659
Size: 1050 271 3975
Fragments arranged by size:
3975 1050 271
NgoMIV G'CCGG_C
Cuts at: 415 641 3473 415
Size: 226 2832 2238
Fragments arranged by size:
2832 2238 226
NruI TCG'CGA
Cuts at: 3657 3657
Size: 5296
NsiI A_TGCA'T
Cuts at: 543 543
Size: 5296
NspI r_CATG'y
Cuts at: 2347 2847 3756 2347
Size: 500 909 3887
Fragments arranged by size:
3887 909 500
PciI A'CATG_T
Cuts at: 3752 3752
Size: 5296
PflMI CCAn_nnn'nTGG
Cuts at: 2205 2205
Size: 5296
PfoI T'CCnGG_A
Cuts at: 1371 1371
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5296
PmlI CAC'GTG
Cuts at: 618 618
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4435 4467 4435
Size: 32 5264
PpuMI rG'GwC_Cy
Cuts at: 1375 1752 1375
Size: 377 4919
PshAI GACnn'nnGTC
Cuts at: 440 440
Size: 5296
PspOMI G'GGCC_C
Cuts at: 740 1241 740
Size: 501 4795
PstI C_TGCA'G
Cuts at: 194 1442 1658 1827 2131 2341 3026 194
Size: 1248 216 169 304 210 685 2464
Fragments arranged by size:
2464 1248 685 304 216 210 169
PvuI CG_AT'CG
Cuts at: 104 554 104
Size: 450 4846
PvuII CAG'CTG
Cuts at: 440 870 2719 3079 440
Size: 430 1849 360 2657
Fragments arranged by size:
2657 1849 430 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5296
SacI G_AGCT'C
Cuts at: 400 1683 2308 400
Size: 1283 625 3388
Fragments arranged by size:
3388 1283 625
SacII CC_GC'GG
Cuts at: 979 1091 1485 979
Size: 112 394 4790
Fragments arranged by size:
4790 394 112
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5296
SapI GCTCTTCn'nnn_
Cuts at: 361 3317 3527 361
Size: 2956 210 2130
Fragments arranged by size:
2956 2130 210
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5296
ScaI AGT'ACT
Cuts at: 775 4813 775
Size: 4038 1258
SexAI A'CCwGG_T
Cuts at: 1613 1613
Size: 5296
SfcI C'TryA_G
Cuts at: 33 190 1438 1654 1823 2127 2337 3022 4017
Size: 157 1248 216 169 304 210 685 995
Cuts at: 4017 4208 33
Size: 191 1121
Fragments arranged by size:
1248 1121 995 685 304 216 210 191
169 157
SfoI GGC'GCC
Cuts at: 420 953 2726 2974 420
Size: 533 1773 248 2742
Fragments arranged by size:
2742 1773 533 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5296
SmaI CCC'GGG
Cuts at: 850 2214 2316 850
Size: 1364 102 3830
Fragments arranged by size:
3830 1364 102
SmlI C'TyrA_G
Cuts at: 2449 3364 3858 4120 2449
Size: 915 494 262 3625
Fragments arranged by size:
3625 915 494 262
SnaBI TAC'GTA
Cuts at: 2286 2286
Size: 5296
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5296
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4796
StyI C'CwwG_G
Cuts at: 659 698 1289 1709 1980 2168 2234 2428 2782
Size: 39 591 420 271 188 66 194 354
Cuts at: 2782 659
Size: 3173
Fragments arranged by size:
3173 591 420 354 271 194 188 66
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1799 1892 2877 3010 3469 3680 1799
Size: 93 985 133 459 211 3415
Fragments arranged by size:
3415 985 459 211 133 93
TatI w'GTAC_w
Cuts at: 773 1937 4811 773
Size: 1164 2874 1258
Fragments arranged by size:
2874 1258 1164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 995 1027 1795 1827 4813 4845 995
Size: 32 768 32 2986 32 1446
Fragments arranged by size:
2986 1446 768 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1276 3091 1276
Size: 1815 3481
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 679 2232 2789 679
Size: 1553 557 3186
Fragments arranged by size:
3186 1553 557
XmaI C'CCGG_G
Cuts at: 848 2212 2314 848
Size: 1364 102 3830
Fragments arranged by size:
3830 1364 102
XmnI GAAnn'nnTTC
Cuts at: 370 370
Size: 5296
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BmgBI BmtI BsmI BsmBI BstEII BstZ17I ClaI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
PacI PasI PsiI PspXI PsrI SanDI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI