Restriction map for
pF1KSDA1242
(Circular) (Six-base) MAPSORT of: pF1KSDA1242.seq Check: 7354 from: 1 to: 4618
pF1KSDA1242 4618 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 754 754
Size: 4618
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4618
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4618
AclI AA'CG_TT
Cuts at: 4458 4458
Size: 4618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2873 3622 3890 2441
Size: 432 749 268 3169
Fragments arranged by size:
3169 749 432 268
AfeI AGC'GCT
Cuts at: 532 532
Size: 4618
AflIII A'CryG_T
Cuts at: 192 942 1968 3074 4051 192
Size: 750 1026 1106 977 759
Fragments arranged by size:
1106 1026 977 759 750
AgeI A'CCGG_T
Cuts at: 3006 3006
Size: 4618
AhdI GACnn_n'nnGTC
Cuts at: 711 711
Size: 4618
AleI CACnn'nnGTG
Cuts at: 748 1305 748
Size: 557 4061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 4618
AlwNI CAG_nnn'CTG
Cuts at: 603 1921 3490 603
Size: 1318 1569 1731
Fragments arranged by size:
1731 1569 1318
ApaLI G'TGCA_C
Cuts at: 3388 3388
Size: 4618
ApoI r'AATT_y
Cuts at: 1620 3971 4227 1620
Size: 2351 256 2011
Fragments arranged by size:
2351 2011 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4618
AvaI C'yCGr_G
Cuts at: 670 1113 1152 1242 1636 670
Size: 443 39 90 394 3652
Fragments arranged by size:
3652 443 394 90 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1251 1284 1251
Size: 33 4585
BamHI G'GATC_C
Cuts at: 1598 1641 1598
Size: 43 4575
BanI G'GyrC_C
Cuts at: 146 427 742 1632 2046 2294 2329 146
Size: 281 315 890 414 248 35 2435
Fragments arranged by size:
2435 890 414 315 281 248 35
BanII G_rGCy'C
Cuts at: 1082 1165 1324 1630 2660 1082
Size: 83 159 306 1030 3040
Fragments arranged by size:
3040 1030 306 159 83
BbeI G_GCGC'C
Cuts at: 150 2050 2298 150
Size: 1900 248 2470
Fragments arranged by size:
2470 1900 248
BciVI GTATCCnnnnn_n'
Cuts at: 1279 2514 3277 4618 1279
Size: 1235 763 1341 1279
Fragments arranged by size:
1341 1279 1235 763
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4618
BglI GCCn_nnn'nGGC
Cuts at: 1201 1201
Size: 4618
BglII A'GATC_T
Cuts at: 635 887 2131 4170 635
Size: 252 1244 2039 1083
Fragments arranged by size:
2039 1244 1083 252
BlpI GC'TnA_GC
Cuts at: 727 968 1728 727
Size: 241 760 3617
Fragments arranged by size:
3617 760 241
Bme1580I G_kGCm'C
Cuts at: 868 1451 2241 2334 3392 868
Size: 583 790 93 1058 2094
Fragments arranged by size:
2094 1058 790 583 93
BmrI ACTGGGnnnn_n'
Cuts at: 229 332 1828 1909 2244 4390 229
Size: 103 1496 81 335 2146 457
Fragments arranged by size:
2146 1496 457 335 103 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 315 519 1532 261
Size: 54 204 1013 3347
Fragments arranged by size:
3347 1013 204 54
Bpu10I CC'TnA_GC
Cuts at: 520 1159 520
Size: 639 3979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1066 1792 2671 3165 3463 394
Size: 672 726 879 494 298 1549
Fragments arranged by size:
1549 879 726 672 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 254 1513 4322 254
Size: 1259 2809 550
Fragments arranged by size:
2809 1259 550
BsaAI yAC'GTr
Cuts at: 512 1608 1969 512
Size: 1096 361 3161
Fragments arranged by size:
3161 1096 361
BsaBI GATnn'nnATC
Cuts at: 6 166 2130 4169 6
Size: 160 1964 2039 455
Fragments arranged by size:
2039 1964 455 160
BsaHI Gr'CG_yC
Cuts at: 147 220 362 2047 2295 2999 4495 147
Size: 73 142 1685 248 704 1496 270
Fragments arranged by size:
1685 1496 704 270 248 142 73
BsaWI w'CCGG_w
Cuts at: 347 608 1601 1805 2326 3006 3280 3427 3838
Size: 261 993 204 521 680 274 147 411
Cuts at: 3838 347
Size: 1127
Fragments arranged by size:
1127 993 680 521 411 274 261 204
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 753 783 1306 1336 1558 1588 753
Size: 30 523 30 222 30 3783
Fragments arranged by size:
3783 523 222 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1096 1096
Size: 4618
BseYI C'CCAG_C
Cuts at: 247 423 2041 2799 3378 3874 247
Size: 176 1618 758 579 496 991
Fragments arranged by size:
1618 991 758 579 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 904 1189 466
Size: 438 285 3895
Fragments arranged by size:
3895 438 285
BsiEI CG_ry'CG
Cuts at: 104 996 1196 2158 2204 3414 104
Size: 892 200 962 46 1210 1308
Fragments arranged by size:
1308 1210 962 892 200 46
BsiHKAI G_wGCw'C
Cuts at: 728 1187 1375 1583 1630 2408 2598 3392 728
Size: 459 188 208 47 778 190 794 1954
Fragments arranged by size:
1954 794 778 459 208 190 188 47
BsmI GAATG_Cn'
Cuts at: 969 969
Size: 4618
BsmBI CGTCTCn'nnnn_
Cuts at: 1508 1508
Size: 4618
Bsp1286I G_dGCh'C
Cuts at: 728 868 1082 1165 1187 1324 1375 1451 1583
Size: 140 214 83 22 137 51 76 132
Cuts at: 1583 1630 2241 2334 2408 2598 2660 3392 728
Size: 47 611 93 74 190 62 732 1954
Fragments arranged by size:
1954 732 611 214 190 140 137 132
93 83 76 74 62 51 47 22
BspEI T'CCGG_A
Cuts at: 3838 3838
Size: 4618
BspHI T'CATG_A
Cuts at: 3794 3794
Size: 4618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 1666 1880 2182 2563 754
Size: 912 214 302 381 2809
Fragments arranged by size:
2809 912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 144 843 1044 2908 2962 4448 4613 50
Size: 94 699 201 1864 54 1486 165 55
Fragments arranged by size:
1864 1486 699 201 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 2528 1481
Size: 1047 3571
BsrFI r'CCGG_y
Cuts at: 430 1061 2614 2795 3006 430
Size: 631 1553 181 211 2042
Fragments arranged by size:
2042 1553 631 211 181
BssSI C'ACGA_G
Cuts at: 2887 3247 2887
Size: 360 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 1233 1993 1233
Size: 760 3858
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4618
BstXI CCAn_nnnn'nTGG
Cuts at: 1365 1365
Size: 4618
BstYI r'GATC_y
Cuts at: 1 635 644 887 1397 1598 1641 2108 2131
Size: 634 9 243 510 201 43 467 23
Cuts at: 2131 2466 2712 3726 3812 3824 4170 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 634 510 467 449 346 335 246
243 201 86 43 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 1525 1525
Size: 4618
BtgI C'CryG_G
Cuts at: 125 827 125
Size: 702 3916
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1191 2566 2747 2910 1191
Size: 1375 181 163 2899
Fragments arranged by size:
2899 1375 181 163
BtsI GCAGTG_nn'
Cuts at: 1030 1300 1312 1859 1030
Size: 270 12 547 3789
Fragments arranged by size:
3789 547 270 12
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4618
DrdI GACnn_nn'nnGTC
Cuts at: 1566 2322 3182 1566
Size: 756 860 3002
Fragments arranged by size:
3002 860 756
EaeI y'GGCC_r
Cuts at: 1193 1433 2201 2375 2766 2793 4140 1193
Size: 240 768 174 391 27 1347 1671
Fragments arranged by size:
1671 1347 768 391 240 174 27
EagI C'GGCC_G
Cuts at: 1193 2201 1193
Size: 1008 3610
EarI CTCTTCn'nnn_
Cuts at: 1594 2639 2849 1594
Size: 1045 210 3363
Fragments arranged by size:
3363 1045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 225 2824 3136 3282 4428 225
Size: 2599 312 146 1146 415
Fragments arranged by size:
2599 1146 415 312 146
EcoICRI GAG'CTC
Cuts at: 1628 1628
Size: 4618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 315 519 1532 2441 2873 3622 3890 261
Size: 54 204 1013 909 432 749 268 989
Fragments arranged by size:
1013 989 909 749 432 268 204 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1426 1426
Size: 4618
EcoO109I rG'GnC_Cy
Cuts at: 450 1755 450
Size: 1305 3313
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 666 2635 666
Size: 1969 2649
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4618
HaeII r_GCGC'y
Cuts at: 150 534 955 2050 2298 3322 4301 150
Size: 384 421 1095 248 1024 979 467
Fragments arranged by size:
1095 1024 979 467 421 384 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 697 729 961 993 1306 1338 1558
Size: 32 313 32 232 32 313 32 220
Cuts at: 1558 1590 2458 2488 2490 2520 352
Size: 32 868 30 2 30 2450
Fragments arranged by size:
2450 868 313 313 232 220 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1655 1655
Size: 4618
KasI G'GCGC_C
Cuts at: 146 2046 2294 146
Size: 1900 248 2470
Fragments arranged by size:
2470 1900 248
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4618
MluI A'CGCG_T
Cuts at: 192 942 4051 192
Size: 750 3109 759
Fragments arranged by size:
3109 759 750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 905 1019 3289 3473 905
Size: 114 2270 184 2050
Fragments arranged by size:
2270 2050 184 114
MscI TGG'CCA
Cuts at: 1435 2377 1435
Size: 942 3676
MslI CAynn'nnrTG
Cuts at: 202 748 1305 2605 2732 202
Size: 546 557 1300 127 2088
Fragments arranged by size:
2088 1300 557 546 127
MspA1I CmG'CkG
Cuts at: 975 1258 1265 1727 2041 2401 3416 3661 975
Size: 283 7 462 314 360 1015 245 1932
Fragments arranged by size:
1932 1015 462 360 314 283 245 7
NaeI GCC'GGC
Cuts at: 432 2797 432
Size: 2365 2253
NarI GG'CG_CC
Cuts at: 147 2047 2295 147
Size: 1900 248 2470
Fragments arranged by size:
2470 1900 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4618
NgoMIV G'CCGG_C
Cuts at: 430 2795 430
Size: 2365 2253
NruI TCG'CGA
Cuts at: 2979 2979
Size: 4618
NspI r_CATG'y
Cuts at: 1669 2169 3078 1669
Size: 500 909 3209
Fragments arranged by size:
3209 909 500
PciI A'CATG_T
Cuts at: 3074 3074
Size: 4618
PflMI CCAn_nnn'nTGG
Cuts at: 1211 1211
Size: 4618
PfoI T'CCnGG_A
Cuts at: 982 982
Size: 4618
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3789 3757
Size: 32 4586
PpuMI rG'GwC_Cy
Cuts at: 450 450
Size: 4618
PspXI vC'TCGA_Gb
Cuts at: 1152 1152
Size: 4618
PstI C_TGCA'G
Cuts at: 479 680 1010 1037 1055 1307 1663 2348 479
Size: 201 330 27 18 252 356 685 2749
Fragments arranged by size:
2749 685 356 330 252 201 27 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4618
PvuII CAG'CTG
Cuts at: 975 1258 2041 2401 975
Size: 283 783 360 3192
Fragments arranged by size:
3192 783 360 283
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4618
SacI G_AGCT'C
Cuts at: 1630 1630
Size: 4618
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4618
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4408
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4618
ScaI AGT'ACT
Cuts at: 4135 4135
Size: 4618
SexAI A'CCwGG_T
Cuts at: 851 851
Size: 4618
SfcI C'TryA_G
Cuts at: 33 475 676 1006 1033 1051 1303 1659 2344
Size: 442 201 330 27 18 252 356 685
Cuts at: 2344 3339 3530 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 685 442 356 330 252 201
191 27 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 1201 1201
Size: 4618
SfoI GGC'GCC
Cuts at: 148 2048 2296 148
Size: 1900 248 2470
Fragments arranged by size:
2470 1900 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4618
SmaI CCC'GGG
Cuts at: 1638 1638
Size: 4618
SmlI C'TyrA_G
Cuts at: 409 1081 1152 1771 2686 3180 3442 409
Size: 672 71 619 915 494 262 1585
Fragments arranged by size:
1585 915 672 619 494 262 71
SnaBI TAC'GTA
Cuts at: 1608 1608
Size: 4618
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4618
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4118
StyI C'CwwG_G
Cuts at: 125 356 1750 2104 125
Size: 231 1394 354 2639
Fragments arranged by size:
2639 1394 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 415 2199 2332 2791 3002 415
Size: 1784 133 459 211 2031
Fragments arranged by size:
2031 1784 459 211 133
TatI w'GTAC_w
Cuts at: 1175 4133 1175
Size: 2958 1660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4135 4167 4135
Size: 32 4586
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4618
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 4618
XhoI C'TCGA_G
Cuts at: 1152 1152
Size: 4618
XmaI C'CCGG_G
Cuts at: 1636 1636
Size: 4618
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AloI ApaI AscI AvrII BbsI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PsrI SacII SanDI SgrAI
SrfI SspI StuI SwaI XmnI ZraI