Restriction map for
pF1KSDA1244
(Circular) (Six-base) MAPSORT of: pF1KSDA1244.seq Check: 3827 from: 1 to: 8654
pF1KSDA1244 8654 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1413 4101 1413
Size: 2688 5966
AatII G_ACGT'C
Cuts at: 2930 2930
Size: 8654
AccI GT'mk_AC
Cuts at: 782 851 5692 782
Size: 69 4841 3744
Fragments arranged by size:
4841 3744 69
Acc65I G'GTAC_C
Cuts at: 5670 5670
Size: 8654
AclI AA'CG_TT
Cuts at: 8494 8494
Size: 8654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1120 1369 1823 2181 2563 3373 3817 4002
Size: 606 249 454 358 382 810 444 185
Cuts at: 4002 4501 4513 5068 5287 5334 6479 6911 7658
Size: 499 12 555 219 47 1145 432 747
Cuts at: 7658 7926 514
Size: 268 1242
Fragments arranged by size:
1242 1145 810 747 606 555 499 454
444 432 382 358 268 249 219 185
47 12
AfeI AGC'GCT
Cuts at: 2259 2259
Size: 8654
AflIII A'CryG_T
Cuts at: 989 2148 2150 2781 6006 7110 8087 989
Size: 1159 2 631 3225 1104 977 1556
Fragments arranged by size:
3225 1556 1159 1104 977 631 2
AgeI A'CCGG_T
Cuts at: 1061 3454 7042 1061
Size: 2393 3588 2673
Fragments arranged by size:
3588 2673 2393
AhdI GACnn_n'nnGTC
Cuts at: 1002 1002
Size: 8654
AleI CACnn'nnGTG
Cuts at: 2005 2005
Size: 8654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1235 1235
Size: 8654
AlwNI CAG_nnn'CTG
Cuts at: 1149 1293 2031 2355 3302 3502 5959 7526 1149
Size: 144 738 324 947 200 2457 1567 2277
Fragments arranged by size:
2457 2277 1567 947 738 324 200 144
ApaI G_GGCC'C
Cuts at: 3901 3901
Size: 8654
ApaLI G'TGCA_C
Cuts at: 7424 7424
Size: 8654
ApoI r'AATT_y
Cuts at: 5658 8007 8263 5658
Size: 2349 256 6049
Fragments arranged by size:
6049 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8654
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8654
AvaI C'yCGr_G
Cuts at: 658 685 975 1043 2278 2299 4474 4725 5018
Size: 27 290 68 1235 21 2175 251 293
Cuts at: 5018 5362 5674 658
Size: 344 312 3638
Fragments arranged by size:
3638 2175 1235 344 312 293 290 251
68 27 21
AvrII C'CTAG_G
Cuts at: 1053 1053
Size: 8654
BamHI G'GATC_C
Cuts at: 5103 5679 5103
Size: 576 8078
BanI G'GyrC_C
Cuts at: 415 1392 2176 2206 4516 5670 6084 6332 6367
Size: 977 784 30 2310 1154 414 248 35
Cuts at: 6367 415
Size: 2702
Fragments arranged by size:
2702 2310 1154 977 784 414 248 35
30
BanII G_rGCy'C
Cuts at: 229 431 1478 1774 2293 3258 3901 5413 5668
Size: 202 1047 296 519 965 643 1512 255
Cuts at: 5668 6698 229
Size: 1030 2185
Fragments arranged by size:
2185 1512 1047 1030 965 643 519 296
255 202
BbeI G_GCGC'C
Cuts at: 1396 4520 6088 6336 1396
Size: 3124 1568 248 3714
Fragments arranged by size:
3714 3124 1568 248
BbsI GAAGACnn'nnnn_
Cuts at: 1443 3390 4697 1443
Size: 1947 1307 5400
Fragments arranged by size:
5400 1947 1307
BbvCI CC'TCA_GC
Cuts at: 1279 1917 2101 2680 1279
Size: 638 184 579 7253
Fragments arranged by size:
7253 638 579 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1836 1870 4341 4375 1836
Size: 34 2471 34 6115
Fragments arranged by size:
6115 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6552 7313 8654 6552
Size: 761 1341 6552
Fragments arranged by size:
6552 1341 761
BclI T'GATC_A
Cuts at: 945 3782 6174 945
Size: 2837 2392 3425
Fragments arranged by size:
3425 2837 2392
BfrBI ATG'CAT
Cuts at: 356 356
Size: 8654
BglI GCCn_nnn'nGGC
Cuts at: 1933 3829 4375 1933
Size: 1896 546 6212
Fragments arranged by size:
6212 1896 546
BglII A'GATC_T
Cuts at: 6169 8206 6169
Size: 2037 6617
BlpI GC'TnA_GC
Cuts at: 430 754 1882 4771 5766 430
Size: 324 1128 2889 995 3318
Fragments arranged by size:
3318 2889 1128 995 324
Bme1580I G_kGCm'C
Cuts at: 420 2179 3018 3243 3901 3942 4758 6279 6372
Size: 1759 839 225 658 41 816 1521 93
Cuts at: 6372 7428 420
Size: 1056 1646
Fragments arranged by size:
1759 1646 1521 1056 839 816 658 225
93 41
BmgBI CAC'GTC
Cuts at: 990 2079 990
Size: 1089 7565
BmrI ACTGGGnnnn_n'
Cuts at: 1982 3214 3684 4125 5281 5866 5947 6282 8426
Size: 1232 470 441 1156 585 81 335 2144
Cuts at: 8426 1982
Size: 2210
Fragments arranged by size:
2210 2144 1232 1156 585 470 441 335
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 181 1907 7021 181
Size: 1726 5114 1814
Fragments arranged by size:
5114 1814 1726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 2053 2134 2203 2751 3083 3505 3837 4270
Size: 1929 81 69 548 332 422 332 433
Cuts at: 4270 4358 4897 5509 124
Size: 88 539 612 3269
Fragments arranged by size:
3269 1929 612 548 539 433 422 332
332 88 81 69
Bpu10I CC'TnA_GC
Cuts at: 1236 1279 1917 2101 2329 2680 5041 5394 1236
Size: 43 638 184 228 351 2361 353 4496
Fragments arranged by size:
4496 2361 638 353 351 228 184 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 505 703 2615 3952 5830 6709 7201 7499
Size: 37 198 1912 1337 1878 879 492 298
Cuts at: 7499 468
Size: 1623
Fragments arranged by size:
1912 1878 1623 1337 879 492 298 198
37
BsaI GGTCTCn'nnnn_
Cuts at: 2994 8358 2994
Size: 5364 3290
BsaAI yAC'GTr
Cuts at: 2151 2172 2718 5568 5646 6007 2151
Size: 21 546 2850 78 361 4798
Fragments arranged by size:
4798 2850 546 361 78 21
BsaBI GATnn'nnATC
Cuts at: 6 6168 8205 6
Size: 6162 2037 455
Fragments arranged by size:
6162 2037 455
BsaHI Gr'CG_yC
Cuts at: 1393 1838 2927 4517 6085 6333 7035 8531 1393
Size: 445 1089 1590 1568 248 702 1496 1516
Fragments arranged by size:
1590 1568 1516 1496 1089 702 445 248
BsaWI w'CCGG_w
Cuts at: 1061 3454 5843 6364 7042 7316 7463 7874 1061
Size: 2393 2389 521 678 274 147 411 1841
Fragments arranged by size:
2393 2389 1841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1620 1650 1620
Size: 30 8624
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 502 621 1150 1207 1330 1486 3610 3928
Size: 192 119 529 57 123 156 2124 318
Cuts at: 3928 4050 4906 310
Size: 122 856 4058
Fragments arranged by size:
4058 2124 856 529 318 192 156 123
122 119 57
BseYI C'CCAG_C
Cuts at: 179 256 411 3243 3987 4106 4667 5080 5107
Size: 77 155 2832 744 119 561 413 27
Cuts at: 5107 5312 5607 6079 6837 7414 7910 179
Size: 205 295 472 758 577 496 923
Fragments arranged by size:
2832 923 758 744 577 561 496 472
413 295 205 155 119 77 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 820 1307 1447 2050 2071 4807 387
Size: 433 487 140 603 21 2736 4234
Fragments arranged by size:
4234 2736 603 487 433 140 21
BsiEI CG_ry'CG
Cuts at: 104 1384 4613 6196 6242 7450 104
Size: 1280 3229 1583 46 1208 1308
Fragments arranged by size:
3229 1583 1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 140 431 1478 1493 1774 2189 2306 2335 2456
Size: 291 1047 15 281 415 117 29 121
Cuts at: 2456 3740 4138 5027 5413 5668 6446 6636 7428
Size: 1284 398 889 386 255 778 190 792
Cuts at: 7428 140
Size: 1366
Fragments arranged by size:
1366 1284 1047 889 792 778 415 398
386 291 281 255 190 121 117 29
15
BsmI GAATG_Cn'
Cuts at: 2676 5139 5343 2676
Size: 2463 204 5987
Fragments arranged by size:
5987 2463 204
BsmBI CGTCTCn'nnnn_
Cuts at: 241 742 5426 241
Size: 501 4684 3469
Fragments arranged by size:
4684 3469 501
Bsp1286I G_dGCh'C
Cuts at: 140 229 420 431 1478 1493 1774 2179 2189
Size: 89 191 11 1047 15 281 405 10
Cuts at: 2189 2293 2306 2335 2456 3018 3243 3258 3740
Size: 104 13 29 121 562 225 15 482
Cuts at: 3740 3901 3942 4138 4758 5027 5413 5668 6279
Size: 161 41 196 620 269 386 255 611
Cuts at: 6279 6372 6446 6636 6698 7428 140
Size: 93 74 190 62 730 1366
Fragments arranged by size:
1366 1047 730 620 611 562 482 405
386 281 269 255 225 196 191 190
161 121 104 93 89 74 62 41
29 15 15 13 11 10
BspEI T'CCGG_A
Cuts at: 7874 7874
Size: 8654
BspHI T'CATG_A
Cuts at: 1415 2423 7830 1415
Size: 1008 5407 2239
Fragments arranged by size:
5407 2239 1008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 971 1413 3992 4101 4436 5075 5704 5918 6220
Size: 442 2579 109 335 639 629 214 302
Cuts at: 6220 6601 971
Size: 381 3024
Fragments arranged by size:
3024 2579 639 629 442 381 335 302
214 109
BsrBI CCG'CTC
Cuts at: 50 1207 1833 2429 5393 6946 7000 8484 8649
Size: 1157 626 596 2964 1553 54 1484 165
Cuts at: 8649 50
Size: 55
Fragments arranged by size:
2964 1553 1484 1157 626 596 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2612 6566 2612
Size: 3954 4700
BsrFI r'CCGG_y
Cuts at: 1061 3370 3454 5053 6652 6833 7042 1061
Size: 2309 84 1599 1599 181 209 2673
Fragments arranged by size:
2673 2309 1599 1599 209 181 84
BssSI C'ACGA_G
Cuts at: 6925 7283 6925
Size: 358 8296
BstAPI GCAn_nnn'nTGC
Cuts at: 471 6031 471
Size: 5560 3094
BstBI TT'CG_AA
Cuts at: 110 7015 110
Size: 6905 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1782 1782
Size: 8654
BstYI r'GATC_y
Cuts at: 1 315 874 2984 5103 5447 5679 6146 6169
Size: 314 559 2110 2119 344 232 467 23
Cuts at: 6169 6504 6750 7762 7848 7860 8206 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2119 2110 1012 559 467 449 346 344
335 314 246 232 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3295 3295
Size: 8654
BtgI C'CryG_G
Cuts at: 524 586 1359 2409 3080 3517 4100 5156 524
Size: 62 773 1050 671 437 583 1056 4022
Fragments arranged by size:
4022 1056 1050 773 671 583 437 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2216 2537 4558 6604 6785 6948 2216
Size: 321 2021 2046 181 163 3922
Fragments arranged by size:
3922 2046 2021 321 181 163
BtsI GCAGTG_nn'
Cuts at: 1326 1599 2069 3414 3614 4678 5540 5897 1326
Size: 273 470 1345 200 1064 862 357 4083
Fragments arranged by size:
4083 1345 1064 862 470 357 273 200
ClaI AT'CG_AT
Cuts at: 1730 1730
Size: 8654
DraI TTT'AAA
Cuts at: 5653 5653
Size: 8654
DraIII CAC_nnn'GTG
Cuts at: 159 189 587 2754 4133 159
Size: 30 398 2167 1379 4680
Fragments arranged by size:
4680 2167 1379 398 30
DrdI GACnn_nn'nnGTC
Cuts at: 2941 6360 7218 2941
Size: 3419 858 4377
Fragments arranged by size:
4377 3419 858
EaeI y'GGCC_r
Cuts at: 1381 1605 2143 2441 3317 3830 4610 6239 6413
Size: 224 538 298 876 513 780 1629 174
Cuts at: 6413 6804 6831 8176 1381
Size: 391 27 1345 1859
Fragments arranged by size:
1859 1629 1345 876 780 538 513 391
298 224 174 27
EagI C'GGCC_G
Cuts at: 1381 4610 6239 1381
Size: 3229 1629 3796
Fragments arranged by size:
3796 3229 1629
EarI CTCTTCn'nnn_
Cuts at: 1025 1961 2884 4307 4735 6677 6887 1025
Size: 936 923 1423 428 1942 210 2792
Fragments arranged by size:
2792 1942 1423 936 923 428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1129 2650 3637 6862 7172 7318 8464 1129
Size: 1521 987 3225 310 146 1146 1319
Fragments arranged by size:
3225 1521 1319 1146 987 310 146
EcoICRI GAG'CTC
Cuts at: 429 1476 1772 5411 5666 429
Size: 1047 296 3639 255 3417
Fragments arranged by size:
3639 3417 1047 296 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 514 1120 1369 1823 2053 2134 2181 2203
Size: 390 606 249 454 230 81 47 22
Cuts at: 2203 2563 2751 3083 3373 3505 3817 3837 4002
Size: 360 188 332 290 132 312 20 165
Cuts at: 4002 4270 4358 4501 4513 4897 5068 5287 5334
Size: 268 88 143 12 384 171 219 47
Cuts at: 5334 5509 6479 6911 7658 7926 124
Size: 175 970 432 747 268 852
Fragments arranged by size:
970 852 747 606 454 432 390 384
360 332 312 290 268 268 249 230
219 188 175 171 165 143 132 88
81 47 47 22 20 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2755 4636 2755
Size: 1881 6773
EcoO109I rG'GnC_Cy
Cuts at: 253 866 2274 2367 3801 3898 3981 4327 4629
Size: 613 1408 93 1434 97 83 346 302
Cuts at: 4629 5064 5275 5359 5387 5793 253
Size: 435 211 84 28 406 3114
Fragments arranged by size:
3114 1434 1408 613 435 406 346 302
211 97 93 84 83 28
EcoRI G'AATT_C
Cuts at: 5658 5658
Size: 8654
EcoRV GAT'ATC
Cuts at: 3765 3765
Size: 8654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 235 3822 4256 6673 235
Size: 3587 434 2417 2216
Fragments arranged by size:
3587 2417 2216 434
FspI TGC'GCA
Cuts at: 6435 6435
Size: 8654
HaeII r_GCGC'y
Cuts at: 1130 1396 2261 2325 2648 3303 4520 6088 6336
Size: 266 865 64 323 655 1217 1568 248
Cuts at: 6336 7358 8337 1130
Size: 1022 979 1447
Fragments arranged by size:
1568 1447 1217 1022 979 865 655 323
266 248 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 441 473 937 969 988 1020 1236 1268 1723
Size: 32 464 32 19 32 216 32 455
Cuts at: 1723 1755 2194 2226 3670 3702 3940 3972 6496
Size: 32 439 32 1444 32 238 32 2524
Cuts at: 6496 6526 6528 6558 441
Size: 30 2 30 2537
Fragments arranged by size:
2537 2524 1444 464 455 439 238 216
32 32 32 32 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 5693 5693
Size: 8654
HindIII A'AGCT_T
Cuts at: 374 3119 3747 374
Size: 2745 628 5281
Fragments arranged by size:
5281 2745 628
KasI G'GCGC_C
Cuts at: 1392 4516 6084 6332 1392
Size: 3124 1568 248 3714
Fragments arranged by size:
3714 3124 1568 248
KpnI G_GTAC'C
Cuts at: 5674 5674
Size: 8654
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 8654
MluI A'CGCG_T
Cuts at: 8087 8087
Size: 8654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 986 1010 2426 2907 7325 7509 986
Size: 24 1416 481 4418 184 2131
Fragments arranged by size:
4418 2131 1416 481 184 24
MscI TGG'CCA
Cuts at: 1607 2145 3319 3832 6415 1607
Size: 538 1174 513 2583 3846
Fragments arranged by size:
3846 2583 1174 538 513
MslI CAynn'nnrTG
Cuts at: 1561 1846 2005 2867 4399 4861 5638 6643 6770
Size: 285 159 862 1532 462 777 1005 127
Cuts at: 6770 1561
Size: 3445
Fragments arranged by size:
3445 1532 1005 862 777 462 285 159
127
MspA1I CmG'CkG
Cuts at: 551 558 1321 1436 1611 1939 2411 2541 2907
Size: 7 763 115 175 328 472 130 366
Cuts at: 2907 3492 3499 3895 4004 4022 5298 5556 5765
Size: 585 7 396 109 18 1276 258 209
Cuts at: 5765 6079 6439 7452 7697 551
Size: 314 360 1013 245 1508
Fragments arranged by size:
1508 1276 1013 763 585 472 396 366
360 328 314 258 245 209 175 130
115 109 18 7 7
NaeI GCC'GGC
Cuts at: 3372 6835 3372
Size: 3463 5191
NarI GG'CG_CC
Cuts at: 1393 4517 6085 6333 1393
Size: 3124 1568 248 3714
Fragments arranged by size:
3714 3124 1568 248
NcoI C'CATG_G
Cuts at: 524 3080 4100 5156 524
Size: 2556 1020 1056 4022
Fragments arranged by size:
4022 2556 1056 1020
NgoMIV G'CCGG_C
Cuts at: 3370 6833 3370
Size: 3463 5191
NsiI A_TGCA'T
Cuts at: 358 358
Size: 8654
NspI r_CATG'y
Cuts at: 356 1506 2785 5707 6207 7114 356
Size: 1150 1279 2922 500 907 1896
Fragments arranged by size:
2922 1896 1279 1150 907 500
PasI CC'CwG_GG
Cuts at: 5279 5279
Size: 8654
PciI A'CATG_T
Cuts at: 2781 7110 2781
Size: 4329 4325
PflMI CCAn_nnn'nTGG
Cuts at: 1614 1753 3595 4572 1614
Size: 139 1842 977 5696
Fragments arranged by size:
5696 1842 977 139
PfoI T'CCnGG_A
Cuts at: 2363 2375 4335 2363
Size: 12 1960 6682
Fragments arranged by size:
6682 1960 12
PmeI GTTT'AAAC
Cuts at: 5653 5653
Size: 8654
PmlI CAC'GTG
Cuts at: 2151 2718 5568 2151
Size: 567 2850 5237
Fragments arranged by size:
5237 2850 567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 7793 7825 1207
Size: 32 6554 32 2036
Fragments arranged by size:
6554 2036 32 32
PpuMI rG'GwC_Cy
Cuts at: 253 866 2274 2367 3801 3981 4629 5064 5275
Size: 613 1408 93 1434 180 648 435 211
Cuts at: 5275 253
Size: 3632
Fragments arranged by size:
3632 1434 1408 648 613 435 211 180
93
PshAI GACnn'nnGTC
Cuts at: 2784 2827 3455 2784
Size: 43 628 7983
Fragments arranged by size:
7983 628 43
PspOMI G'GGCC_C
Cuts at: 3897 3897
Size: 8654
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5134 5166 5134
Size: 32 8622
PstI C_TGCA'G
Cuts at: 859 1214 5701 6386 859
Size: 355 4487 685 3127
Fragments arranged by size:
4487 3127 685 355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8654
PvuII CAG'CTG
Cuts at: 1321 1611 1939 2541 2907 3492 5556 6079 6439
Size: 290 328 602 366 585 2064 523 360
Cuts at: 6439 1321
Size: 3536
Fragments arranged by size:
3536 2064 602 585 523 366 360 328
290
RsrII CG'GwC_CG
Cuts at: 6849 6849
Size: 8654
SacI G_AGCT'C
Cuts at: 431 1478 1774 5413 5668 431
Size: 1047 296 3639 255 3417
Fragments arranged by size:
3639 3417 1047 296 255
SacII CC_GC'GG
Cuts at: 2412 2412
Size: 8654
SalI G'TCGA_C
Cuts at: 5691 5691
Size: 8654
SanDI GG'GwC_CC
Cuts at: 2274 5275 2274
Size: 3001 5653
SapI GCTCTTCn'nnn_
Cuts at: 1025 6677 6887 1025
Size: 5652 210 2792
Fragments arranged by size:
5652 2792 210
SbfI CC_TGCA'GG
Cuts at: 5701 5701
Size: 8654
ScaI AGT'ACT
Cuts at: 952 4084 8171 952
Size: 3132 4087 1435
Fragments arranged by size:
4087 3132 1435
SexAI A'CCwGG_T
Cuts at: 2750 2936 3062 2750
Size: 186 126 8342
Fragments arranged by size:
8342 186 126
SfcI C'TryA_G
Cuts at: 33 855 1210 1371 1628 5697 6382 7375 7566
Size: 822 355 161 257 4069 685 993 191
Cuts at: 7566 33
Size: 1121
Fragments arranged by size:
4069 1121 993 822 685 355 257 191
161
SfoI GGC'GCC
Cuts at: 1394 4518 6086 6334 1394
Size: 3124 1568 248 3714
Fragments arranged by size:
3714 3124 1568 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8654
SmaI CCC'GGG
Cuts at: 1045 4476 5020 5676 1045
Size: 3431 544 656 4023
Fragments arranged by size:
4023 3431 656 544
SmlI C'TyrA_G
Cuts at: 447 484 658 718 2630 3967 5809 6724 7216
Size: 37 174 60 1912 1337 1842 915 492
Cuts at: 7216 7478 447
Size: 262 1623
Fragments arranged by size:
1912 1842 1623 1337 915 492 262 174
60 37
SnaBI TAC'GTA
Cuts at: 5646 5646
Size: 8654
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8654
SphI G_CATG'C
Cuts at: 356 5707 6207 356
Size: 5351 500 2803
Fragments arranged by size:
5351 2803 500
SspI AAT'ATT
Cuts at: 3350 3350
Size: 8654
StuI AGG'CCT
Cuts at: 1157 1462 2111 1157
Size: 305 649 7700
Fragments arranged by size:
7700 649 305
StyI C'CwwG_G
Cuts at: 524 1053 2268 2444 2519 3080 4100 5156 5328
Size: 529 1215 176 75 561 1020 1056 172
Cuts at: 5328 5788 6142 524
Size: 460 354 3036
Fragments arranged by size:
3036 1215 1056 1020 561 529 460 354
176 172 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 403 1585 2651 4144 4791 5206 5587 6237
Size: 42 1182 1066 1493 647 415 381 650
Cuts at: 6237 6370 6829 7038 361
Size: 133 459 209 1977
Fragments arranged by size:
1977 1493 1182 1066 650 647 459 415
381 209 133 42
TatI w'GTAC_w
Cuts at: 950 4082 8169 950
Size: 3132 4087 1435
Fragments arranged by size:
4087 3132 1435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 4117 4149 4156 4188 5209 5241 8171
Size: 32 1819 32 7 32 1021 32 2930
Cuts at: 8171 8203 2266
Size: 32 2717
Fragments arranged by size:
2930 2717 1819 1021 32 32 32 32
32 7
Tth111I GACn'n_nGTC
Cuts at: 864 2371 6451 864
Size: 1507 4080 3067
Fragments arranged by size:
4080 3067 1507
XbaI T'CTAG_A
Cuts at: 5685 5685
Size: 8654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2248 2958 3855 5088 6149 2200
Size: 48 710 897 1233 1061 4705
Fragments arranged by size:
4705 1233 1061 897 710 48
XhoI C'TCGA_G
Cuts at: 658 658
Size: 8654
XmaI C'CCGG_G
Cuts at: 1043 4474 5018 5674 1043
Size: 3431 544 656 4023
Fragments arranged by size:
4023 3431 656 544
XmnI GAAnn'nnTTC
Cuts at: 1411 3122 1411
Size: 1711 6943
ZraI GAC'GTC
Cuts at: 2928 2928
Size: 8654
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI BaeI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I CspCI FseI FspAI HpaI NdeI NheI
NotI NruI PacI PsiI PspXI SfiI SgrAI SrfI
SwaI