Restriction map for
pF1KSDA1246
(Circular) (Six-base) MAPSORT of: pF1KSDA1246.seq Check: 518 from: 1 to: 5506
pF1KSDA1246 5506 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1207 1207
Size: 5506
AatII G_ACGT'C
Cuts at: 2185 2185
Size: 5506
AccI GT'mk_AC
Cuts at: 179 2492 2542 179
Size: 2313 50 3143
Fragments arranged by size:
3143 2313 50
Acc65I G'GTAC_C
Cuts at: 2520 2520
Size: 5506
AclI AA'CG_TT
Cuts at: 5346 5346
Size: 5506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 3329 3761 4510 4778 1433
Size: 1896 432 749 268 2161
Fragments arranged by size:
2161 1896 749 432 268
AflIII A'CryG_T
Cuts at: 2856 3962 4939 2856
Size: 1106 977 3423
Fragments arranged by size:
3423 1106 977
AgeI A'CCGG_T
Cuts at: 3894 3894
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 640 640
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1050 1050
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 475 2431 475
Size: 1956 3550
AlwNI CAG_nnn'CTG
Cuts at: 213 720 759 1579 1713 2809 4378 213
Size: 507 39 820 134 1096 1569 1341
Fragments arranged by size:
1569 1341 1096 820 507 134 39
ApaI G_GGCC'C
Cuts at: 1908 1921 2040 2228 2448 1908
Size: 13 119 188 220 4966
Fragments arranged by size:
4966 220 188 119 13
ApaLI G'TGCA_C
Cuts at: 4276 4276
Size: 5506
ApoI r'AATT_y
Cuts at: 2508 4859 5115 2508
Size: 2351 256 2899
Fragments arranged by size:
2899 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5506
AvaI C'yCGr_G
Cuts at: 421 892 1466 2189 2237 2447 2524 421
Size: 471 574 723 48 210 77 3403
Fragments arranged by size:
3403 723 574 471 210 77 48
BamHI G'GATC_C
Cuts at: 2048 2529 2048
Size: 481 5025
BanI G'GyrC_C
Cuts at: 697 1150 1189 1720 1867 2520 2934 3182 3217
Size: 453 39 531 147 653 414 248 35
Cuts at: 3217 697
Size: 2986
Fragments arranged by size:
2986 653 531 453 414 248 147 39
35
BanII G_rGCy'C
Cuts at: 1908 1921 1949 2040 2228 2448 2518 3548 1908
Size: 13 28 91 188 220 70 1030 3866
Fragments arranged by size:
3866 1030 220 188 91 70 28 13
BbeI G_GCGC'C
Cuts at: 1871 2938 3186 1871
Size: 1067 248 4191
Fragments arranged by size:
4191 1067 248
BbsI GAAGACnn'nnnn_
Cuts at: 1761 1761
Size: 5506
BbvCI CC'TCA_GC
Cuts at: 1270 1270
Size: 5506
BciVI GTATCCnnnnn_n'
Cuts at: 3402 4165 5506 3402
Size: 763 1341 3402
Fragments arranged by size:
3402 1341 763
BclI T'GATC_A
Cuts at: 3024 3024
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 305 1225 1969 305
Size: 920 744 3842
Fragments arranged by size:
3842 920 744
BglII A'GATC_T
Cuts at: 3019 5058 3019
Size: 2039 3467
BlpI GC'TnA_GC
Cuts at: 658 2616 658
Size: 1958 3548
Bme1580I G_kGCm'C
Cuts at: 241 700 1908 1921 2040 2228 2448 3129 3222
Size: 459 1208 13 119 188 220 681 93
Cuts at: 3222 4280 241
Size: 1058 1467
Fragments arranged by size:
1467 1208 1058 681 459 220 188 119
93 13
BmrI ACTGGGnnnn_n'
Cuts at: 235 1098 1653 2716 2797 3132 5278 235
Size: 863 555 1063 81 335 2146 463
Fragments arranged by size:
2146 1063 863 555 463 335 81
BmtI G_CTAG'C
Cuts at: 154 154
Size: 5506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3871 3871
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1314 1980 2064 2136 2144 1048
Size: 266 666 84 72 8 4410
Fragments arranged by size:
4410 666 266 84 72 8
Bpu10I CC'TnA_GC
Cuts at: 1270 1270
Size: 5506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1033 1110 2104 2680 3559 4053 4351 922
Size: 111 77 994 576 879 494 298 2077
Fragments arranged by size:
2077 994 879 576 494 298 111 77
BsaI GGTCTCn'nnnn_
Cuts at: 124 1251 2373 5210 124
Size: 1127 1122 2837 420
Fragments arranged by size:
2837 1127 1122 420
BsaAI yAC'GTr
Cuts at: 2496 2857 2496
Size: 361 5145
BsaBI GATnn'nnATC
Cuts at: 6 3018 5057 6
Size: 3012 2039 455
Fragments arranged by size:
3012 2039 455
BsaHI Gr'CG_yC
Cuts at: 182 1868 2182 2935 3183 3887 5383 182
Size: 1686 314 753 248 704 1496 305
Fragments arranged by size:
1686 1496 753 704 314 305 248
BsaWI w'CCGG_w
Cuts at: 2693 3214 3894 4168 4315 4726 2693
Size: 521 680 274 147 411 3473
Fragments arranged by size:
3473 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 2321 2351 964
Size: 30 1327 30 4119
Fragments arranged by size:
4119 1327 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 982 985 1113 1977 2152 2348 2440 982
Size: 3 128 864 175 196 92 4048
Fragments arranged by size:
4048 864 196 175 128 92 3
BseYI C'CCAG_C
Cuts at: 531 1001 1072 1533 1819 1871 2028 2077 2176
Size: 470 71 461 286 52 157 49 99
Cuts at: 2176 2220 2929 3687 4266 4762 531
Size: 44 709 758 579 496 1275
Fragments arranged by size:
1275 758 709 579 496 470 461 286
157 99 71 52 49 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1277
Size: 5506
BsiEI CG_ry'CG
Cuts at: 104 1838 3046 3092 4302 104
Size: 1734 1208 46 1210 1308
Fragments arranged by size:
1734 1308 1210 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1949 2488 2518 3296 3486 4280 1949
Size: 539 30 778 190 794 3175
Fragments arranged by size:
3175 794 778 539 190 30
Bsp1286I G_dGCh'C
Cuts at: 241 700 1908 1921 1949 2040 2228 2448 2488
Size: 459 1208 13 28 91 188 220 40
Cuts at: 2488 2518 3129 3222 3296 3486 3548 4280 241
Size: 30 611 93 74 190 62 732 1467
Fragments arranged by size:
1467 1208 732 611 459 220 190 188
93 91 74 62 40 30 28 13
BspEI T'CCGG_A
Cuts at: 4726 4726
Size: 5506
BspHI T'CATG_A
Cuts at: 4682 4682
Size: 5506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 683 719 1207 2554 2768 3070 3451 509
Size: 174 36 488 1347 214 302 381 2564
Fragments arranged by size:
2564 1347 488 381 302 214 174 36
BsrBI CCG'CTC
Cuts at: 50 434 785 897 3796 3850 5336 5501 50
Size: 384 351 112 2899 54 1486 165 55
Fragments arranged by size:
2899 1486 384 351 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 1202 1851 3416 1202
Size: 649 1565 3292
Fragments arranged by size:
3292 1565 649
BsrFI r'CCGG_y
Cuts at: 278 3502 3683 3894 278
Size: 3224 181 211 1890
Fragments arranged by size:
3224 1890 211 181
BssSI C'ACGA_G
Cuts at: 1810 3775 4135 1810
Size: 1965 360 3181
Fragments arranged by size:
3181 1965 360
BstAPI GCAn_nnn'nTGC
Cuts at: 759 2881 759
Size: 2122 3384
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5506
BstEII G'GTnAC_C
Cuts at: 847 847
Size: 5506
BstXI CCAn_nnnn'nTGG
Cuts at: 1236 1647 1731 1764 1827 2004 1236
Size: 411 84 33 63 177 4738
Fragments arranged by size:
4738 411 177 84 63 33
BstYI r'GATC_y
Cuts at: 1 582 732 2048 2529 2996 3019 3354 3600
Size: 581 150 1316 481 467 23 335 246
Cuts at: 3600 4614 4700 4712 5058 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1316 1014 581 481 467 449 346 335
246 150 86 23 12
BtgI C'CryG_G
Cuts at: 125 173 609 619 1229 1235 125
Size: 48 436 10 610 6 4396
Fragments arranged by size:
4396 610 436 48 10 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 525 672 3454 3635 3798 525
Size: 147 2782 181 163 2233
Fragments arranged by size:
2782 2233 181 163 147
BtsI GCAGTG_nn'
Cuts at: 858 1289 1365 1693 2016 2215 2747 858
Size: 431 76 328 323 199 532 3617
Fragments arranged by size:
3617 532 431 328 323 199 76
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 798 833 798
Size: 35 5471
DraI TTT'AAA
Cuts at: 2503 2503
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 3210 4070 3210
Size: 860 4646
EaeI y'GGCC_r
Cuts at: 1040 1232 1601 1634 1754 1835 3089 3263 3654
Size: 192 369 33 120 81 1254 174 391
Cuts at: 3654 3681 5028 1040
Size: 27 1347 1518
Fragments arranged by size:
1518 1347 1254 391 369 192 174 120
81 33 27
EagI C'GGCC_G
Cuts at: 1835 3089 1835
Size: 1254 4252
EarI CTCTTCn'nnn_
Cuts at: 1995 3527 3737 1995
Size: 1532 210 3764
Fragments arranged by size:
3764 1532 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 297 1382 2049 3712 4024 4170 5316 297
Size: 1085 667 1663 312 146 1146 487
Fragments arranged by size:
1663 1146 1085 667 487 312 146
EcoICRI GAG'CTC
Cuts at: 1947 2516 1947
Size: 569 4937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1314 1433 1980 2064 2136 2144 3329 3761
Size: 266 119 547 84 72 8 1185 432
Cuts at: 3761 4510 4778 1048
Size: 749 268 1776
Fragments arranged by size:
1776 1185 749 547 432 268 266 119
84 72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 472 472
Size: 5506
EcoO109I rG'GnC_Cy
Cuts at: 230 489 933 1068 1815 1904 1905 1917 2037
Size: 259 444 135 747 89 1 12 120
Cuts at: 2037 2099 2224 2225 2444 2643 230
Size: 62 125 1 219 199 3093
Fragments arranged by size:
3093 747 444 259 219 199 135 125
120 89 62 12 1 1
EcoRI G'AATT_C
Cuts at: 2508 2508
Size: 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3523 3523
Size: 5506
FspI TGC'GCA
Cuts at: 1939 3285 1939
Size: 1346 4160
HaeII r_GCGC'y
Cuts at: 1871 2938 3186 4210 5189 1871
Size: 1067 248 1024 979 2188
Fragments arranged by size:
2188 1067 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 724 756 3346 3376 3378 3408 626
Size: 32 66 32 2590 30 2 30 2724
Fragments arranged by size:
2724 2590 66 32 32 30 30 2
HincII GTy'rAC
Cuts at: 180 498 645 1560 2403 2543 180
Size: 318 147 915 843 140 3143
Fragments arranged by size:
3143 915 843 318 147 140
KasI G'GCGC_C
Cuts at: 1867 2934 3182 1867
Size: 1067 248 4191
Fragments arranged by size:
4191 1067 248
KpnI G_GTAC'C
Cuts at: 2524 2524
Size: 5506
MfeI C'AATT_G
Cuts at: 864 864
Size: 5506
MluI A'CGCG_T
Cuts at: 4939 4939
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1565 2246 4177 4361 1565
Size: 681 1931 184 2710
Fragments arranged by size:
2710 1931 681 184
MscI TGG'CCA
Cuts at: 1603 1756 3265 1603
Size: 153 1509 3844
Fragments arranged by size:
3844 1509 153
MslI CAynn'nnrTG
Cuts at: 962 1050 1234 1750 1783 3493 3620 962
Size: 88 184 516 33 1710 127 2848
Fragments arranged by size:
2848 1710 516 184 127 88 33
MspA1I CmG'CkG
Cuts at: 531 657 1135 1260 1485 1834 2615 2929 3289
Size: 126 478 125 225 349 781 314 360
Cuts at: 3289 4304 4549 531
Size: 1015 245 1488
Fragments arranged by size:
1488 1015 781 478 360 349 314 245
225 126 125
NaeI GCC'GGC
Cuts at: 3685 3685
Size: 5506
NarI GG'CG_CC
Cuts at: 1868 2935 3183 1868
Size: 1067 248 4191
Fragments arranged by size:
4191 1067 248
NcoI C'CATG_G
Cuts at: 125 609 1235 125
Size: 484 626 4396
Fragments arranged by size:
4396 626 484
NgoMIV G'CCGG_C
Cuts at: 3683 3683
Size: 5506
NheI G'CTAG_C
Cuts at: 150 150
Size: 5506
NruI TCG'CGA
Cuts at: 3867 3867
Size: 5506
NspI r_CATG'y
Cuts at: 961 2412 2557 3057 3966 961
Size: 1451 145 500 909 2501
Fragments arranged by size:
2501 1451 909 500 145
PasI CC'CwG_GG
Cuts at: 928 2004 928
Size: 1076 4430
PciI A'CATG_T
Cuts at: 3962 3962
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 469 1713 469
Size: 1244 4262
PfoI T'CCnGG_A
Cuts at: 376 1949 2152 376
Size: 1573 203 3730
Fragments arranged by size:
3730 1573 203
PmeI GTTT'AAAC
Cuts at: 2503 2503
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4645 4677 4645
Size: 32 5474
PpuMI rG'GwC_Cy
Cuts at: 230 1068 230
Size: 838 4668
PshAI GACnn'nnGTC
Cuts at: 1135 1135
Size: 5506
PspOMI G'GGCC_C
Cuts at: 1904 1917 2036 2224 2444 1904
Size: 13 119 188 220 4966
Fragments arranged by size:
4966 220 188 119 13
PspXI vC'TCGA_Gb
Cuts at: 892 892
Size: 5506
PstI C_TGCA'G
Cuts at: 506 716 755 1490 2551 3236 506
Size: 210 39 735 1061 685 2776
Fragments arranged by size:
2776 1061 735 685 210 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5506
PvuII CAG'CTG
Cuts at: 531 657 1260 1485 2929 3289 531
Size: 126 603 225 1444 360 2748
Fragments arranged by size:
2748 1444 603 360 225 126
RsrII CG'GwC_CG
Cuts at: 2205 3699 2205
Size: 1494 4012
SacI G_AGCT'C
Cuts at: 1949 2518 1949
Size: 569 4937
SalI G'TCGA_C
Cuts at: 178 2541 178
Size: 2363 3143
SanDI GG'GwC_CC
Cuts at: 230 1068 230
Size: 838 4668
SapI GCTCTTCn'nnn_
Cuts at: 3527 3737 3527
Size: 210 5296
SbfI CC_TGCA'GG
Cuts at: 2551 2551
Size: 5506
ScaI AGT'ACT
Cuts at: 196 5023 196
Size: 4827 679
SexAI A'CCwGG_T
Cuts at: 1565 2435 1565
Size: 870 4636
SfcI C'TryA_G
Cuts at: 33 502 712 751 1486 2349 2547 3232 4227
Size: 469 210 39 735 863 198 685 995
Cuts at: 4227 4418 33
Size: 191 1121
Fragments arranged by size:
1121 995 863 735 685 469 210 198
191 39
SfoI GGC'GCC
Cuts at: 1869 2936 3184 1869
Size: 1067 248 4191
Fragments arranged by size:
4191 1067 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5506
SmaI CCC'GGG
Cuts at: 1468 2191 2239 2449 2526 1468
Size: 723 48 210 77 4448
Fragments arranged by size:
4448 723 210 77 48
SmlI C'TyrA_G
Cuts at: 421 892 937 1048 1125 2119 2659 3574 4068
Size: 471 45 111 77 994 540 915 494
Cuts at: 4068 4330 421
Size: 262 1597
Fragments arranged by size:
1597 994 915 540 494 471 262 111
77 45
SnaBI TAC'GTA
Cuts at: 2496 2496
Size: 5506
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5506
SphI G_CATG'C
Cuts at: 2412 2557 3057 2412
Size: 145 500 4861
Fragments arranged by size:
4861 500 145
SrfI GCCC'GGGC
Cuts at: 2239 2239
Size: 5506
StuI AGG'CCT
Cuts at: 1549 1549
Size: 5506
StyI C'CwwG_G
Cuts at: 125 245 468 609 1235 1670 2638 2992 125
Size: 120 223 141 626 435 968 354 2639
Fragments arranged by size:
2639 968 626 435 354 223 141 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 2222 2463 3087 3220 3679 3890 1015
Size: 1207 241 624 133 459 211 2631
Fragments arranged by size:
2631 1207 624 459 241 211 133
TatI w'GTAC_w
Cuts at: 194 1490 1850 5021 194
Size: 1296 360 3171 679
Fragments arranged by size:
3171 1296 679 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 2321 2353 5023 5055 964
Size: 32 1325 32 2670 32 1415
Fragments arranged by size:
2670 1415 1325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 372 3301 372
Size: 2929 2577
XbaI T'CTAG_A
Cuts at: 2535 2535
Size: 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1236 1431 1644 2999 1236
Size: 195 213 1355 3743
Fragments arranged by size:
3743 1355 213 195
XhoI C'TCGA_G
Cuts at: 421 892 421
Size: 471 5035
XmaI C'CCGG_G
Cuts at: 1466 2189 2237 2447 2524 1466
Size: 723 48 210 77 4448
Fragments arranged by size:
4448 723 210 77 48
ZraI GAC'GTC
Cuts at: 2183 2183
Size: 5506
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BcgI BfrBI
BmgBI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NotI NsiI PacI PmlI PsiI PsrI SacII SfiI
SgrAI SspI SwaI XmnI