Restriction map for
pF1KSDA1253
(Circular) (Six-base) MAPSORT of: pF1KSDA1253.seq Check: 7425 from: 1 to: 4496
pF1KSDA1253 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 589 589
Size: 4496
AccI GT'mk_AC
Cuts at: 779 932 996 1534 779
Size: 153 64 538 3741
Fragments arranged by size:
3741 538 153 64
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2321 2753 3500 3768 902
Size: 1419 432 747 268 1630
Fragments arranged by size:
1630 1419 747 432 268
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 1801 3368 1801
Size: 1567 2929
ApaLI G'TGCA_C
Cuts at: 487 3266 487
Size: 2779 1717
ApoI r'AATT_y
Cuts at: 511 1500 3849 4105 511
Size: 989 2349 256 902
Fragments arranged by size:
2349 989 902 256
AseI AT'TA_AT
Cuts at: 20 1183 20
Size: 1163 3333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 696 1516 696
Size: 820 3676
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 601 1452 1512 1926 2174 2209 601
Size: 851 60 414 248 35 2888
Fragments arranged by size:
2888 851 414 248 60 35
BanII G_rGCy'C
Cuts at: 1510 2540 1510
Size: 1030 3466
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 908 908
Size: 4496
BciVI GTATCCnnnnn_n'
Cuts at: 158 2394 3155 4496 158
Size: 2236 761 1341 158
Fragments arranged by size:
2236 1341 761 158
BclI T'GATC_A
Cuts at: 449 2016 449
Size: 1567 2929
BglII A'GATC_T
Cuts at: 898 2011 4048 898
Size: 1113 2037 1346
Fragments arranged by size:
2037 1346 1113
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 189 491 2121 2214 3270 189
Size: 302 1630 93 1056 1415
Fragments arranged by size:
1630 1415 1056 302 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1240
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1672 2551 3043 3341 1055
Size: 617 879 492 298 2210
Fragments arranged by size:
2210 879 617 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1488 1849 1488
Size: 361 4135
BsaBI GATnn'nnATC
Cuts at: 6 1423 2010 4047 6
Size: 1417 587 2037 455
Fragments arranged by size:
2037 1417 587 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 263 293 263
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 829
Size: 4496
BseYI C'CCAG_C
Cuts at: 1921 2679 3256 3752 1921
Size: 758 577 496 2665
Fragments arranged by size:
2665 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 151 491 1510 2288 2478 3270 151
Size: 340 1019 778 190 792 1377
Fragments arranged by size:
1377 1019 792 778 340 190
BsmI GAATG_Cn'
Cuts at: 544 544
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 151 189 491 1510 2121 2214 2288 2478 2540
Size: 38 302 1019 611 93 74 190 62
Cuts at: 2540 3270 151
Size: 730 1377
Fragments arranged by size:
1377 1019 730 611 302 190 93 74
62 38
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 1325 3672 1325
Size: 2347 2149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 1546 1760 2062 2443 589
Size: 957 214 302 381 2642
Fragments arranged by size:
2642 957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BssSI C'ACGA_G
Cuts at: 1364 1447 2767 3125 1364
Size: 83 1320 358 2735
Fragments arranged by size:
2735 1320 358 83
BstAPI GCAn_nnn'nTGC
Cuts at: 526 1873 526
Size: 1347 3149
BstBI TT'CG_AA
Cuts at: 110 2857 110
Size: 2747 1749
BstYI r'GATC_y
Cuts at: 1 898 1521 1988 2011 2346 2592 3604 3690
Size: 897 623 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1012 897 623 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 152 125
Size: 27 4469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 474 491 1739 474
Size: 17 1248 3231
Fragments arranged by size:
3231 1248 17
DraI TTT'AAA
Cuts at: 510 1495 510
Size: 985 3511
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 454 1413 2519 2729 454
Size: 959 1106 210 2221
Fragments arranged by size:
2221 1106 959 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1240 2321 2753 3500 3768 902
Size: 338 1081 432 747 268 1630
Fragments arranged by size:
1630 1081 747 432 338 268
EcoO109I rG'GnC_Cy
Cuts at: 1635 1635
Size: 4496
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3200 4179 1930
Size: 248 1022 979 2247
Fragments arranged by size:
2247 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1076 1108 1362 1394 2338 2368 2370 2400 1076
Size: 32 254 32 944 30 2 30 3172
Fragments arranged by size:
3172 944 254 32 32 30 30 2
HincII GTy'rAC
Cuts at: 831 1535 831
Size: 704 3792
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MfeI C'AATT_G
Cuts at: 524 524
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 249 1396 3167 3351 249
Size: 1147 1771 184 1394
Fragments arranged by size:
1771 1394 1147 184
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 174 654 2485 2612 174
Size: 480 1831 127 2058
Fragments arranged by size:
2058 1831 480 127
MspA1I CmG'CkG
Cuts at: 1390 1607 1921 2281 3294 3539 1390
Size: 217 314 360 1013 245 2347
Fragments arranged by size:
2347 1013 360 314 245 217
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 125 152 125
Size: 27 4469
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 837 1069 1549 2049 2956 837
Size: 232 480 500 907 2377
Fragments arranged by size:
2377 907 500 480 232
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 901 901
Size: 4496
PfoI T'CCnGG_A
Cuts at: 330 330
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PshAI GACnn'nnGTC
Cuts at: 1390 1390
Size: 4496
PstI C_TGCA'G
Cuts at: 486 522 1543 2228 486
Size: 36 1021 685 2754
Fragments arranged by size:
2754 1021 685 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1390 1921 2281 1390
Size: 531 360 3605
Fragments arranged by size:
3605 531 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 482 518 728 1247 1539 2224 3217 3408
Size: 449 36 210 519 292 685 993 191
Cuts at: 3408 33
Size: 1121
Fragments arranged by size:
1121 993 685 519 449 292 210 191
36
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 696 1070 1651 2566 3058 3320 696
Size: 374 581 915 492 262 1872
Fragments arranged by size:
1872 915 581 492 374 262
SnaBI TAC'GTA
Cuts at: 1488 1488
Size: 4496
SpeI A'CTAG_T
Cuts at: 67 627 67
Size: 560 3936
SphI G_CATG'C
Cuts at: 1069 1549 2049 1069
Size: 480 500 3516
Fragments arranged by size:
3516 500 480
StyI C'CwwG_G
Cuts at: 125 152 1630 1984 125
Size: 27 1478 354 2637
Fragments arranged by size:
2637 1478 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1386 2079 2212 2671 2880 1386
Size: 693 133 459 209 3002
Fragments arranged by size:
3002 693 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 951 2293 951
Size: 1342 3154
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XhoI C'TCGA_G
Cuts at: 696 696
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XmnI ZraI