Restriction map for
pF1KSDA1258
(Circular) (Six-base) MAPSORT of: pF1KSDA1258.seq Check: 7454 from: 1 to: 4499
pF1KSDA1258 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1452 1452
Size: 4499
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1215 2324 2756 3503 3771 1215
Size: 1109 432 747 268 1943
Fragments arranged by size:
1943 1109 747 432 268
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AleI CACnn'nnGTG
Cuts at: 913 913
Size: 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 981 430
Size: 551 3948
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 449
Size: 32 4467
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 601 1306 1503 3852 4108 601
Size: 705 197 2349 256 992
Fragments arranged by size:
2349 992 705 256 197
AseI AT'TA_AT
Cuts at: 20 1190 20
Size: 1170 3329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1100 1133 1100
Size: 33 4466
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 1513 2543 1513
Size: 1030 3469
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 1221 1221
Size: 4499
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 1322 2019 1322
Size: 697 3802
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 1048 1611 1048
Size: 563 3936
Bme1580I G_kGCm'C
Cuts at: 2124 2217 3273 2124
Size: 93 1056 3350
Fragments arranged by size:
3350 1056 93
BmgBI CAC'GTC
Cuts at: 1116 1116
Size: 4499
BmrI ACTGGGnnnn_n'
Cuts at: 811 887 1711 1792 2127 4271 811
Size: 76 824 81 335 2144 1039
Fragments arranged by size:
2144 1039 824 335 81 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1675 2554 3046 3344 1675
Size: 879 492 298 2830
Fragments arranged by size:
2830 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 664 4203 664
Size: 3539 960
BsaAI yAC'GTr
Cuts at: 1491 1852 1491
Size: 361 4138
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 1022 214
Size: 808 3691
BseYI C'CCAG_C
Cuts at: 1104 1924 2682 3259 3755 1104
Size: 820 758 577 496 1848
Fragments arranged by size:
1848 820 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3273 1513
Size: 778 190 792 2739
Fragments arranged by size:
2739 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1513 2124 2217 2291 2481 2543 3273 1513
Size: 611 93 74 190 62 730 2739
Fragments arranged by size:
2739 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 422 1452 1549 1763 2065 2446 202
Size: 220 1030 97 214 302 381 2255
Fragments arranged by size:
2255 1030 381 302 220 214 97
BsrBI CCG'CTC
Cuts at: 50 136 2791 2845 4329 4494 50
Size: 86 2655 54 1484 165 55
Fragments arranged by size:
2655 1484 165 86 55 54
BsrDI GCAATG_nn'
Cuts at: 809 2411 809
Size: 1602 2897
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 632 1876 632
Size: 1244 3255
BstBI TT'CG_AA
Cuts at: 110 2860 110
Size: 2750 1749
BstYI r'GATC_y
Cuts at: 1 1524 1991 2014 2349 2595 3607 3693 3705
Size: 1523 467 23 335 246 1012 86 12
Cuts at: 3705 4051 1
Size: 346 449
Fragments arranged by size:
1523 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 200 200
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1002 2449 2630 2793 1002
Size: 1447 181 163 2708
Fragments arranged by size:
2708 1447 181 163
BtsI GCAGTG_nn'
Cuts at: 1059 1169 1742 1059
Size: 110 573 3816
Fragments arranged by size:
3816 573 110
ClaI AT'CG_AT
Cuts at: 685 685
Size: 4499
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 287 2084 2258 2649 2676 4021 287
Size: 1797 174 391 27 1345 765
Fragments arranged by size:
1797 1345 765 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 1152 1433 2522 2732 1152
Size: 281 1089 210 2919
Fragments arranged by size:
2919 1089 281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1469 2707 3017 3163 4309 1469
Size: 1238 310 146 1146 1659
Fragments arranged by size:
1659 1238 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1215 2324 2756 3503 3771 1215
Size: 1109 432 747 268 1943
Fragments arranged by size:
1943 1109 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1001 1033 2341 2371 2373 2403 1001
Size: 32 1308 30 2 30 3097
Fragments arranged by size:
3097 1308 32 30 30 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1364 3170 3354 1364
Size: 1806 184 2509
Fragments arranged by size:
2509 1806 184
MscI TGG'CCA
Cuts at: 289 2260 289
Size: 1971 2528
MslI CAynn'nnrTG
Cuts at: 205 374 848 913 2488 2615 205
Size: 169 474 65 1575 127 2089
Fragments arranged by size:
2089 1575 474 169 127 65
MspA1I CmG'CkG
Cuts at: 242 612 1610 1924 2284 3297 3542 242
Size: 370 998 314 360 1013 245 1199
Fragments arranged by size:
1199 1013 998 370 360 314 245
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 200 200
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 1552 2052 2959 1552
Size: 500 907 3092
Fragments arranged by size:
3092 907 500
PacI TTA_AT'TAA
Cuts at: 1190 1190
Size: 4499
PasI CC'CwG_GG
Cuts at: 1263 1263
Size: 4499
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PflMI CCAn_nnn'nTGG
Cuts at: 296 1263 296
Size: 967 3532
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PshAI GACnn'nnGTC
Cuts at: 175 175
Size: 4499
PsiI TTA'TAA
Cuts at: 899 899
Size: 4499
PstI C_TGCA'G
Cuts at: 456 981 1546 2231 456
Size: 525 565 685 2724
Fragments arranged by size:
2724 685 565 525
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1924 2284 1924
Size: 360 4139
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 1152 2522 2732 1152
Size: 1370 210 2919
Fragments arranged by size:
2919 1370 210
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 452 977 1542 2227 3220 3411 33
Size: 419 525 565 685 993 191 1121
Fragments arranged by size:
1121 993 685 565 525 419 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 1654 2569 3061 3323 1654
Size: 915 492 262 2830
Fragments arranged by size:
2830 915 492 262
SnaBI TAC'GTA
Cuts at: 1491 1491
Size: 4499
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
SspI AAT'ATT
Cuts at: 1434 1434
Size: 4499
StyI C'CwwG_G
Cuts at: 200 1633 1987 200
Size: 1433 354 2712
Fragments arranged by size:
2712 1433 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 2082 2215 2674 2883 784
Size: 1298 133 459 209 2400
Fragments arranged by size:
2400 1298 459 209 133
TatI w'GTAC_w
Cuts at: 440 4014 440
Size: 3574 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1448 1480 4016 4048 1448
Size: 32 2536 32 1899
Fragments arranged by size:
2536 1899 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 736 1530 736
Size: 794 3705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 425 1994 425
Size: 1569 2930
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 994 994
Size: 4499
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BbvCI
BcgI BfrBI BglI BmtI BpmI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI