Restriction map for
pF1KSDA1259
(Circular) (Six-base) MAPSORT of: pF1KSDA1259.seq Check: 7054 from: 1 to: 7805
pF1KSDA1259 7805 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1857 1857
Size: 7805
AccI GT'mk_AC
Cuts at: 1855 4843 1855
Size: 2988 4817
Acc65I G'GTAC_C
Cuts at: 4821 4821
Size: 7805
AclI AA'CG_TT
Cuts at: 7645 7645
Size: 7805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 502 857 1001 1501 2827 3259 5630 6062
Size: 327 355 144 500 1326 432 2371 432
Cuts at: 6062 6809 7077 175
Size: 747 268 903
Fragments arranged by size:
2371 1326 903 747 500 432 432 355
327 268 144
AflIII A'CryG_T
Cuts at: 4568 5157 6261 7238 4568
Size: 589 1104 977 5135
Fragments arranged by size:
5135 1104 977 589
AgeI A'CCGG_T
Cuts at: 6193 6193
Size: 7805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 962 4237 4279 4615 962
Size: 3275 42 336 4152
Fragments arranged by size:
4152 3275 336 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 4679 4711 2455
Size: 32 2192 32 5549
Fragments arranged by size:
5549 2192 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1479 2442 3053 5110 6677 1479
Size: 963 611 2057 1567 2607
Fragments arranged by size:
2607 2057 1567 963 611
ApaI G_GGCC'C
Cuts at: 210 3288 3756 210
Size: 3078 468 4259
Fragments arranged by size:
4259 3078 468
ApaLI G'TGCA_C
Cuts at: 6575 6575
Size: 7805
ApoI r'AATT_y
Cuts at: 357 459 757 1900 2248 2468 4364 4809 7158
Size: 102 298 1143 348 220 1896 445 2349
Cuts at: 7158 7414 357
Size: 256 748
Fragments arranged by size:
2349 1896 1143 748 445 348 298 256
220 102
AseI AT'TA_AT
Cuts at: 20 1736 2771 20
Size: 1716 1035 5054
Fragments arranged by size:
5054 1716 1035
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7805
AvaI C'yCGr_G
Cuts at: 4307 4825 4307
Size: 518 7287
BamHI G'GATC_C
Cuts at: 324 4830 324
Size: 4506 3299
BanI G'GyrC_C
Cuts at: 142 731 1871 3808 4771 4821 5235 5483 5518
Size: 589 1140 1937 963 50 414 248 35
Cuts at: 5518 142
Size: 2429
Fragments arranged by size:
2429 1937 1140 963 589 414 248 50
35
BanII G_rGCy'C
Cuts at: 210 2205 2506 3288 3756 4819 5849 210
Size: 1995 301 782 468 1063 1030 2166
Fragments arranged by size:
2166 1995 1063 1030 782 468 301
BbeI G_GCGC'C
Cuts at: 5239 5487 5239
Size: 248 7557
BbsI GAAGACnn'nnnn_
Cuts at: 560 1388 1975 2486 2673 3916 560
Size: 828 587 511 187 1243 4449
Fragments arranged by size:
4449 1243 828 587 511 187
BbvCI CC'TCA_GC
Cuts at: 2356 4293 2356
Size: 1937 5868
BciVI GTATCCnnnnn_n'
Cuts at: 1363 5703 6464 7805 1363
Size: 4340 761 1341 1363
Fragments arranged by size:
4340 1363 1341 761
BclI T'GATC_A
Cuts at: 5325 5325
Size: 7805
BglI GCCn_nnn'nGGC
Cuts at: 1096 1570 1795 1804 1096
Size: 474 225 9 7097
Fragments arranged by size:
7097 474 225 9
BglII A'GATC_T
Cuts at: 3581 4202 5320 7357 3581
Size: 621 1118 2037 4029
Fragments arranged by size:
4029 2037 1118 621
BlpI GC'TnA_GC
Cuts at: 970 4917 970
Size: 3947 3858
Bme1580I G_kGCm'C
Cuts at: 210 1134 3288 3756 5430 5523 6579 210
Size: 924 2154 468 1674 93 1056 1436
Fragments arranged by size:
2154 1674 1436 1056 924 468 93
BmgBI CAC'GTC
Cuts at: 4396 4396
Size: 7805
BmrI ACTGGGnnnn_n'
Cuts at: 309 1601 3676 5017 5098 5433 7577 309
Size: 1292 2075 1341 81 335 2144 537
Fragments arranged by size:
2144 2075 1341 1292 537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6172 6172
Size: 7805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1980 2801 3365 3544 3680 4805 401
Size: 1579 821 564 179 136 1125 3401
Fragments arranged by size:
3401 1579 1125 821 564 179 136
Bpu10I CC'TnA_GC
Cuts at: 2087 2356 3762 4293 2087
Size: 269 1406 531 5599
Fragments arranged by size:
5599 1406 531 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 3448 3455 4981 5860 6352 6650 2080
Size: 1368 7 1526 879 492 298 3235
Fragments arranged by size:
3235 1526 1368 879 492 298 7
BsaI GGTCTCn'nnnn_
Cuts at: 2615 2753 7509 2615
Size: 138 4756 2911
Fragments arranged by size:
4756 2911 138
BsaAI yAC'GTr
Cuts at: 4797 5158 4797
Size: 361 7444
BsaBI GATnn'nnATC
Cuts at: 6 2312 4207 5319 7356 6
Size: 2306 1895 1112 2037 455
Fragments arranged by size:
2306 2037 1895 1112 455
BsaHI Gr'CG_yC
Cuts at: 5236 5484 6186 7682 5236
Size: 248 702 1496 5359
Fragments arranged by size:
5359 1496 702 248
BsaWI w'CCGG_w
Cuts at: 212 4754 4994 5515 6193 6467 6614 7025 212
Size: 4542 240 521 678 274 147 411 992
Fragments arranged by size:
4542 992 678 521 411 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1409 1412 1439 1442 2595 2625 4216 4246 4273
Size: 3 27 3 1153 30 1591 30 27
Cuts at: 4273 4303 4305 4335 1409
Size: 30 2 30 4879
Fragments arranged by size:
4879 1591 1153 30 30 30 30 27
27 3 3 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1433 2373 3102 4281 1433
Size: 940 729 1179 4957
Fragments arranged by size:
4957 1179 940 729
BseYI C'CCAG_C
Cuts at: 4284 5230 5988 6565 7061 4284
Size: 946 758 577 496 5028
Fragments arranged by size:
5028 946 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 707 2050 3236 4207 147
Size: 560 1343 1186 971 3745
Fragments arranged by size:
3745 1343 1186 971 560
BsiEI CG_ry'CG
Cuts at: 104 5347 5393 6601 104
Size: 5243 46 1208 1308
Fragments arranged by size:
5243 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1196 4819 5597 5787 6579 1196
Size: 3623 778 190 792 2422
Fragments arranged by size:
3623 2422 792 778 190
BsmI GAATG_Cn'
Cuts at: 509 951 1491 1742 3824 509
Size: 442 540 251 2082 4490
Fragments arranged by size:
4490 2082 540 442 251
BsmBI CGTCTCn'nnnn_
Cuts at: 4350 4350
Size: 7805
Bsp1286I G_dGCh'C
Cuts at: 210 1134 1196 2205 2506 3288 3756 4819 5430
Size: 924 62 1009 301 782 468 1063 611
Cuts at: 5430 5523 5597 5787 5849 6579 210
Size: 93 74 190 62 730 1436
Fragments arranged by size:
1436 1063 1009 924 782 730 611 468
301 190 93 74 62 62
BspEI T'CCGG_A
Cuts at: 4754 7025 4754
Size: 2271 5534
BspHI T'CATG_A
Cuts at: 1319 1344 1932 2540 6981 1319
Size: 25 588 608 4441 2143
Fragments arranged by size:
4441 2143 608 588 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1857 3768 4855 5069 5371 5752 1857
Size: 1911 1087 214 302 381 3910
Fragments arranged by size:
3910 1911 1087 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6097 6151 7635 7800 50
Size: 6047 54 1484 165 55
Fragments arranged by size:
6047 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 361 1784 3210 4508 5717 361
Size: 1423 1426 1298 1209 2449
Fragments arranged by size:
2449 1426 1423 1298 1209
BsrFI r'CCGG_y
Cuts at: 3791 5803 5984 6193 3791
Size: 2012 181 209 5403
Fragments arranged by size:
5403 2012 209 181
BssSI C'ACGA_G
Cuts at: 1127 2693 3170 3653 6076 6434 1127
Size: 1566 477 483 2423 358 2498
Fragments arranged by size:
2498 2423 1566 483 477 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4492 5182 4492
Size: 690 7115
BstBI TT'CG_AA
Cuts at: 110 6166 110
Size: 6056 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 3915 4242 3915
Size: 327 7478
BstYI r'GATC_y
Cuts at: 1 324 2123 3581 4202 4830 5297 5320 5655
Size: 323 1799 1458 621 628 467 23 335
Cuts at: 5655 5901 6913 6999 7011 7357 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1799 1458 1012 628 621 467 449 346
335 323 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1369 2956 1369
Size: 1587 6218
BtgI C'CryG_G
Cuts at: 125 2186 3743 4481 125
Size: 2061 1557 738 3449
Fragments arranged by size:
3449 2061 1557 738
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5755 5936 6099 5755
Size: 181 163 7461
Fragments arranged by size:
7461 181 163
BtsI GCAGTG_nn'
Cuts at: 3068 3188 3274 3680 4221 4263 4497 5048 3068
Size: 120 86 406 541 42 234 551 5825
Fragments arranged by size:
5825 551 541 406 234 120 86 42
ClaI AT'CG_AT
Cuts at: 1406 1406
Size: 7805
DraI TTT'AAA
Cuts at: 1467 4804 1467
Size: 3337 4468
DrdI GACnn_nn'nnGTC
Cuts at: 3085 4267 4750 5511 6369 3085
Size: 1182 483 761 858 4521
Fragments arranged by size:
4521 1182 858 761 483
EaeI y'GGCC_r
Cuts at: 1712 2855 3161 5390 5564 5955 5982 7327 1712
Size: 1143 306 2229 174 391 27 1345 2190
Fragments arranged by size:
2229 2190 1345 1143 391 306 174 27
EagI C'GGCC_G
Cuts at: 5390 5390
Size: 7805
EarI CTCTTCn'nnn_
Cuts at: 810 3841 3935 3968 4662 5828 6038 810
Size: 3031 94 33 694 1166 210 2577
Fragments arranged by size:
3031 2577 1166 694 210 94 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4575 6013 6323 6469 7615 4575
Size: 1438 310 146 1146 4765
Fragments arranged by size:
4765 1438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4817 4817
Size: 7805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 401 502 857 1001 1501 1980 2801 2827
Size: 226 101 355 144 500 479 821 26
Cuts at: 2827 3259 3365 3544 3680 4805 5630 6062 6809
Size: 432 106 179 136 1125 825 432 747
Cuts at: 6809 7077 175
Size: 268 903
Fragments arranged by size:
1125 903 825 821 747 500 479 432
432 355 268 226 179 144 136 106
101 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1144 2895 3523 3558 1144
Size: 1751 628 35 5391
Fragments arranged by size:
5391 1751 628 35
EcoO109I rG'GnC_Cy
Cuts at: 206 207 1476 1828 2168 3284 3285 3483 3752
Size: 1 1269 352 340 1116 1 198 269
Cuts at: 3752 4944 206
Size: 1192 3067
Fragments arranged by size:
3067 1269 1192 1116 352 340 269 198
1 1
EcoRI G'AATT_C
Cuts at: 4809 4809
Size: 7805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1137 2145 4673 5824 1137
Size: 1008 2528 1151 3118
Fragments arranged by size:
3118 2528 1151 1008
FspI TGC'GCA
Cuts at: 5586 5586
Size: 7805
HaeII r_GCGC'y
Cuts at: 2095 2929 4655 5239 5487 6509 7488 2095
Size: 834 1726 584 248 1022 979 2412
Fragments arranged by size:
2412 1726 1022 979 834 584 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3130 3162 3559 3591 3656 3688 4273 4305 5647
Size: 32 397 32 65 32 585 32 1342
Cuts at: 5647 5677 5679 5709 3130
Size: 30 2 30 5226
Fragments arranged by size:
5226 1342 585 397 65 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1431 4844 1431
Size: 3413 4392
HindIII A'AGCT_T
Cuts at: 1013 1051 1217 1013
Size: 38 166 7601
Fragments arranged by size:
7601 166 38
HpaI GTT'AAC
Cuts at: 1431 1431
Size: 7805
KasI G'GCGC_C
Cuts at: 5235 5483 5235
Size: 248 7557
KpnI G_GTAC'C
Cuts at: 4825 4825
Size: 7805
MfeI C'AATT_G
Cuts at: 1866 1866
Size: 7805
MluI A'CGCG_T
Cuts at: 7238 7238
Size: 7805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 1217 2098 2125 2766 3926 4157 6476 6660
Size: 1020 881 27 641 1160 231 2319 184
Cuts at: 6660 197
Size: 1342
Fragments arranged by size:
2319 1342 1160 1020 881 641 231 184
27
MscI TGG'CCA
Cuts at: 1714 2857 3163 5566 1714
Size: 1143 306 2403 3953
Fragments arranged by size:
3953 2403 1143 306
MslI CAynn'nnrTG
Cuts at: 3634 4168 5794 5921 3634
Size: 534 1626 127 5518
Fragments arranged by size:
5518 1626 534 127
MspA1I CmG'CkG
Cuts at: 942 1230 1561 2022 2052 3017 3096 3343 3861
Size: 288 331 461 30 965 79 247 518
Cuts at: 3861 4387 4531 4543 4916 5230 5590 6603 6848
Size: 526 144 12 373 314 360 1013 245
Cuts at: 6848 942
Size: 1899
Fragments arranged by size:
1899 1013 965 526 518 461 373 360
331 314 288 247 245 144 79 30
12
NaeI GCC'GGC
Cuts at: 5986 5986
Size: 7805
NarI GG'CG_CC
Cuts at: 5236 5484 5236
Size: 248 7557
NcoI C'CATG_G
Cuts at: 125 2186 3743 125
Size: 2061 1557 4187
Fragments arranged by size:
4187 2061 1557
NdeI CA'TA_TG
Cuts at: 705 705
Size: 7805
NgoMIV G'CCGG_C
Cuts at: 5984 5984
Size: 7805
NspI r_CATG'y
Cuts at: 606 4266 4858 5358 6265 606
Size: 3660 592 500 907 2146
Fragments arranged by size:
3660 2146 907 592 500
PciI A'CATG_T
Cuts at: 6261 6261
Size: 7805
PfoI T'CCnGG_A
Cuts at: 1355 1355
Size: 7805
PmeI GTTT'AAAC
Cuts at: 4804 4804
Size: 7805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 563 595 6944 6976 563
Size: 32 6349 32 1392
Fragments arranged by size:
6349 1392 32 32
PpuMI rG'GwC_Cy
Cuts at: 1828 2168 3483 1828
Size: 340 1315 6150
Fragments arranged by size:
6150 1315 340
PshAI GACnn'nnGTC
Cuts at: 237 871 237
Size: 634 7171
PspOMI G'GGCC_C
Cuts at: 206 3284 3752 206
Size: 3078 468 4259
Fragments arranged by size:
4259 3078 468
PstI C_TGCA'G
Cuts at: 3303 3690 4392 4530 4542 4604 4852 5537 3303
Size: 387 702 138 12 62 248 685 5571
Fragments arranged by size:
5571 702 685 387 248 138 62 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7805
PvuII CAG'CTG
Cuts at: 942 1561 2022 3017 3343 4387 4543 5230 5590
Size: 619 461 995 326 1044 156 687 360
Cuts at: 5590 942
Size: 3157
Fragments arranged by size:
3157 1044 995 687 619 461 360 326
156
RsrII CG'GwC_CG
Cuts at: 6000 6000
Size: 7805
SacI G_AGCT'C
Cuts at: 4819 4819
Size: 7805
SalI G'TCGA_C
Cuts at: 4842 4842
Size: 7805
SanDI GG'GwC_CC
Cuts at: 2168 2168
Size: 7805
SapI GCTCTTCn'nnn_
Cuts at: 5828 6038 5828
Size: 210 7595
SbfI CC_TGCA'GG
Cuts at: 4852 4852
Size: 7805
ScaI AGT'ACT
Cuts at: 196 679 7322 196
Size: 483 6643 679
Fragments arranged by size:
6643 679 483
SexAI A'CCwGG_T
Cuts at: 3080 4097 3080
Size: 1017 6788
SfcI C'TryA_G
Cuts at: 33 430 681 2895 3299 3686 4388 4526 4538
Size: 397 251 2214 404 387 702 138 12
Cuts at: 4538 4600 4848 5533 6526 6717 33
Size: 62 248 685 993 191 1121
Fragments arranged by size:
2214 1121 993 702 685 404 397 387
251 248 191 138 62 12
SfoI GGC'GCC
Cuts at: 5237 5485 5237
Size: 248 7557
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7805
SmaI CCC'GGG
Cuts at: 4827 4827
Size: 7805
SmlI C'TyrA_G
Cuts at: 2095 3463 3470 4307 4960 5875 6367 6629 2095
Size: 1368 7 837 653 915 492 262 3271
Fragments arranged by size:
3271 1368 915 837 653 492 262 7
SnaBI TAC'GTA
Cuts at: 4797 4797
Size: 7805
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7805
SphI G_CATG'C
Cuts at: 4858 5358 4858
Size: 500 7305
SspI AAT'ATT
Cuts at: 261 261
Size: 7805
StuI AGG'CCT
Cuts at: 692 692
Size: 7805
StyI C'CwwG_G
Cuts at: 125 1145 1764 2186 2267 3743 3839 4939 5293
Size: 1020 619 422 81 1476 96 1100 354
Cuts at: 5293 125
Size: 2637
Fragments arranged by size:
2637 1476 1100 1020 619 422 354 96
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 3224 5388 5521 5980 6189 130
Size: 3094 2164 133 459 209 1746
Fragments arranged by size:
3094 2164 1746 459 209 133
TatI w'GTAC_w
Cuts at: 194 677 1160 1776 7320 194
Size: 483 483 616 5544 679
Fragments arranged by size:
5544 679 616 483 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 4216 4248 7322 7354 3314
Size: 32 870 32 3074 32 3765
Fragments arranged by size:
3765 3074 870 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1524 4397 5602 1524
Size: 2873 1205 3727
Fragments arranged by size:
3727 2873 1205
XbaI T'CTAG_A
Cuts at: 3930 4836 3930
Size: 906 6899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2019 5300 2019
Size: 3281 4524
XhoI C'TCGA_G
Cuts at: 4307 4307
Size: 7805
XmaI C'CCGG_G
Cuts at: 4825 4825
Size: 7805
XmnI GAAnn'nnTTC
Cuts at: 593 3824 593
Size: 3231 4574
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
CspCI DraIII EcoRV FseI FspAI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspXI PsrI
SacII SfiI SgrAI SrfI SwaI ZraI