Restriction map for
pF1KSDA1260
(Circular) (Six-base) MAPSORT of: pF1KSDA1260.seq Check: 9637 from: 1 to: 5585
pF1KSDA1260 5585 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 2398
Size: 5585
AatII G_ACGT'C
Cuts at: 1169 1676 1169
Size: 507 5078
AccI GT'mk_AC
Cuts at: 2573 2623 2573
Size: 50 5535
Acc65I G'GTAC_C
Cuts at: 2601 2601
Size: 5585
AclI AA'CG_TT
Cuts at: 5425 5425
Size: 5585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 1376 1632 2502 3410 3842 4589 4857 1276
Size: 100 256 870 908 432 747 268 2004
Fragments arranged by size:
2004 908 870 747 432 268 256 100
AflIII A'CryG_T
Cuts at: 2937 4041 5018 2937
Size: 1104 977 3504
Fragments arranged by size:
3504 1104 977
AgeI A'CCGG_T
Cuts at: 763 3973 763
Size: 3210 2375
AleI CACnn'nnGTG
Cuts at: 819 2351 819
Size: 1532 4053
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1744 1776 1744
Size: 32 5553
AlwNI CAG_nnn'CTG
Cuts at: 144 465 1191 2098 2890 4457 144
Size: 321 726 907 792 1567 1272
Fragments arranged by size:
1567 1272 907 792 726 321
ApaLI G'TGCA_C
Cuts at: 4355 4355
Size: 5585
ApoI r'AATT_y
Cuts at: 2541 2589 4938 5194 2541
Size: 48 2349 256 2932
Fragments arranged by size:
2932 2349 256 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 5585
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
AvaI C'yCGr_G
Cuts at: 882 2605 882
Size: 1723 3862
BamHI G'GATC_C
Cuts at: 2610 2610
Size: 5585
BanI G'GyrC_C
Cuts at: 664 967 1996 2234 2252 2601 3015 3263 3298
Size: 303 1029 238 18 349 414 248 35
Cuts at: 3298 664
Size: 2951
Fragments arranged by size:
2951 1029 414 349 303 248 238 35
18
BanII G_rGCy'C
Cuts at: 797 1103 1646 2599 3629 797
Size: 306 543 953 1030 2753
Fragments arranged by size:
2753 1030 953 543 306
BbeI G_GCGC'C
Cuts at: 2238 2256 3019 3267 2238
Size: 18 763 248 4556
Fragments arranged by size:
4556 763 248 18
BbsI GAAGACnn'nnnn_
Cuts at: 563 1605 563
Size: 1042 4543
BciVI GTATCCnnnnn_n'
Cuts at: 269 516 1746 3483 4244 5585 269
Size: 247 1230 1737 761 1341 269
Fragments arranged by size:
1737 1341 1230 761 269 247
BclI T'GATC_A
Cuts at: 606 719 3105 606
Size: 113 2386 3086
Fragments arranged by size:
3086 2386 113
BglI GCCn_nnn'nGGC
Cuts at: 2170 2170
Size: 5585
BglII A'GATC_T
Cuts at: 320 3100 5137 320
Size: 2780 2037 768
Fragments arranged by size:
2780 2037 768
BlpI GC'TnA_GC
Cuts at: 1681 2697 1681
Size: 1016 4569
Bme1580I G_kGCm'C
Cuts at: 457 587 667 3210 3303 4359 457
Size: 130 80 2543 93 1056 1683
Fragments arranged by size:
2543 1683 1056 130 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 1413 2252 2797 2878 3213 5357 1413
Size: 839 545 81 335 2144 1641
Fragments arranged by size:
2144 1641 839 545 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3952 3952
Size: 5585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 395
Size: 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2761 3640 4132 4430 2761
Size: 879 492 298 3916
Fragments arranged by size:
3916 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 943 5289 943
Size: 4346 1239
BsaAI yAC'GTr
Cuts at: 582 2577 2938 582
Size: 1995 361 3229
Fragments arranged by size:
3229 1995 361
BsaBI GATnn'nnATC
Cuts at: 6 1733 3099 5136 6
Size: 1727 1366 2037 455
Fragments arranged by size:
2037 1727 1366 455
BsaHI Gr'CG_yC
Cuts at: 1166 1235 1297 1673 2173 2235 2253 2449 3016
Size: 69 62 376 500 62 18 196 567
Cuts at: 3016 3264 3966 5462 1166
Size: 248 702 1496 1289
Fragments arranged by size:
1496 1289 702 567 500 376 248 196
69 62 62 18
BsaWI w'CCGG_w
Cuts at: 763 2774 3295 3973 4247 4394 4805 763
Size: 2011 521 678 274 147 411 1543
Fragments arranged by size:
2011 1543 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 844 2170 844
Size: 1326 4259
BseYI C'CCAG_C
Cuts at: 458 889 983 1230 1570 1574 2040 3010 3768
Size: 431 94 247 340 4 466 970 758
Cuts at: 3768 4345 4841 458
Size: 577 496 1202
Fragments arranged by size:
1202 970 758 577 496 466 431 340
247 94 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 1486
Size: 5585
BsiEI CG_ry'CG
Cuts at: 104 2425 3127 3173 4381 104
Size: 2321 702 46 1208 1308
Fragments arranged by size:
2321 1308 1208 702 46
BsiHKAI G_wGCw'C
Cuts at: 1103 1646 2599 3377 3567 4359 1103
Size: 543 953 778 190 792 2329
Fragments arranged by size:
2329 953 792 778 543 190
BsmI GAATG_Cn'
Cuts at: 1078 1078
Size: 5585
BsmBI CGTCTCn'nnnn_
Cuts at: 1420 1420
Size: 5585
Bsp1286I G_dGCh'C
Cuts at: 457 587 667 797 1103 1646 2599 3210 3303
Size: 130 80 130 306 543 953 611 93
Cuts at: 3303 3377 3567 3629 4359 457
Size: 74 190 62 730 1683
Fragments arranged by size:
1683 953 730 611 543 306 190 130
130 93 80 74 62
BspEI T'CCGG_A
Cuts at: 4805 4805
Size: 5585
BspHI T'CATG_A
Cuts at: 603 1694 4761 603
Size: 1091 3067 1427
Fragments arranged by size:
3067 1427 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1508 2398 2635 2849 3151 3532 1508
Size: 890 237 214 302 381 3561
Fragments arranged by size:
3561 890 381 302 237 214
BsrBI CCG'CTC
Cuts at: 50 965 3877 3931 5415 5580 50
Size: 915 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 915 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 3497 2159
Size: 1338 4247
BsrFI r'CCGG_y
Cuts at: 667 763 1002 1149 2420 3583 3764 3973 667
Size: 96 239 147 1271 1163 181 209 2279
Fragments arranged by size:
2279 1271 1163 239 209 181 147 96
BsrGI T'GTAC_A
Cuts at: 1394 1394
Size: 5585
BssSI C'ACGA_G
Cuts at: 2347 3856 4214 2347
Size: 1509 358 3718
Fragments arranged by size:
3718 1509 358
BstAPI GCAn_nnn'nTGC
Cuts at: 492 2499 2962 492
Size: 2007 463 3115
Fragments arranged by size:
3115 2007 463
BstBI TT'CG_AA
Cuts at: 110 3946 110
Size: 3836 1749
BstEII G'GTnAC_C
Cuts at: 766 766
Size: 5585
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1332 1065
Size: 267 5318
BstYI r'GATC_y
Cuts at: 1 320 475 649 1187 2610 3077 3100 3435
Size: 319 155 174 538 1423 467 23 335
Cuts at: 3435 3681 4693 4779 4791 5137 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1423 1012 538 467 449 346 335 319
246 174 155 86 23 12
BstZ17I GTA'TAC
Cuts at: 2574 2574
Size: 5585
Bsu36I CC'TnA_GG
Cuts at: 1748 2099 1748
Size: 351 5234
BtgI C'CryG_G
Cuts at: 587 642 1058 1484 1590 1688 2550 587
Size: 55 416 426 106 98 862 3622
Fragments arranged by size:
3622 862 426 416 106 98 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3535 3716 3879 3535
Size: 181 163 5241
Fragments arranged by size:
5241 181 163
BtsI GCAGTG_nn'
Cuts at: 814 995 1548 2828 814
Size: 181 553 1280 3571
Fragments arranged by size:
3571 1280 553 181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1865 1900 1865
Size: 35 5550
DraI TTT'AAA
Cuts at: 2584 2584
Size: 5585
DrdI GACnn_nn'nnGTC
Cuts at: 1165 2110 3291 4149 1165
Size: 945 1181 858 2601
Fragments arranged by size:
2601 1181 945 858
EaeI y'GGCC_r
Cuts at: 1004 1124 1487 2023 3170 3344 3735 3762 5107
Size: 120 363 536 1147 174 391 27 1345
Cuts at: 5107 1004
Size: 1482
Fragments arranged by size:
1482 1345 1147 536 391 363 174 120
27
EagI C'GGCC_G
Cuts at: 3170 3170
Size: 5585
EarI CTCTTCn'nnn_
Cuts at: 946 1651 2188 2300 3608 3818 946
Size: 705 537 112 1308 210 2713
Fragments arranged by size:
2713 1308 705 537 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1449 3793 4103 4249 5395 1449
Size: 2344 310 146 1146 1639
Fragments arranged by size:
2344 1639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1101 1644 2597 1101
Size: 543 953 4089
Fragments arranged by size:
4089 953 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1276 1376 1632 2502 3410 3842 4589 4857
Size: 881 100 256 870 908 432 747 268
Cuts at: 4857 395
Size: 1123
Fragments arranged by size:
1123 908 881 870 747 432 268 256
100
EcoO109I rG'GnC_Cy
Cuts at: 294 351 855 893 1602 2079 2095 2724 294
Size: 57 504 38 709 477 16 629 3155
Fragments arranged by size:
3155 709 629 504 477 57 38 16
EcoRI G'AATT_C
Cuts at: 2589 2589
Size: 5585
EcoRV GAT'ATC
Cuts at: 2286 2286
Size: 5585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 580 3604 580
Size: 3024 2561
FspI TGC'GCA
Cuts at: 3366 3366
Size: 5585
HaeII r_GCGC'y
Cuts at: 1688 2212 2238 2256 3019 3267 4289 5268 1688
Size: 524 26 18 763 248 1022 979 2005
Fragments arranged by size:
2005 1022 979 763 524 248 26 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 460 492 857 889 1966 1998 2299
Size: 32 116 32 365 32 1077 32 301
Cuts at: 2299 2301 2331 2333 2341 2373 3427 3457 3459
Size: 2 30 2 8 32 1054 30 2
Cuts at: 3459 3489 312
Size: 30 2408
Fragments arranged by size:
2408 1077 1054 365 301 116 32 32
32 32 32 30 30 30 8 2
2 2
HincII GTy'rAC
Cuts at: 212 270 914 1239 2624 212
Size: 58 644 325 1385 3173
Fragments arranged by size:
3173 1385 644 325 58
HpaI GTT'AAC
Cuts at: 212 212
Size: 5585
KasI G'GCGC_C
Cuts at: 2234 2252 3015 3263 2234
Size: 18 763 248 4556
Fragments arranged by size:
4556 763 248 18
KpnI G_GTAC'C
Cuts at: 2605 2605
Size: 5585
MluI A'CGCG_T
Cuts at: 5018 5018
Size: 5585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1349 1877 4256 4440 1349
Size: 528 2379 184 2494
Fragments arranged by size:
2494 2379 528 184
MscI TGG'CCA
Cuts at: 1489 2025 3346 1489
Size: 536 1321 3728
Fragments arranged by size:
3728 1321 536
MslI CAynn'nnrTG
Cuts at: 467 580 819 1063 1595 1624 2351 2464 3574
Size: 113 239 244 532 29 727 113 1110
Cuts at: 3574 3701 467
Size: 127 2351
Fragments arranged by size:
2351 1110 727 532 244 239 127 113
113 29
MspA1I CmG'CkG
Cuts at: 983 1574 2334 2696 3010 3370 4383 4628 983
Size: 591 760 362 314 360 1013 245 1940
Fragments arranged by size:
1940 1013 760 591 362 360 314 245
NaeI GCC'GGC
Cuts at: 1004 3766 1004
Size: 2762 2823
NarI GG'CG_CC
Cuts at: 2235 2253 3016 3264 2235
Size: 18 763 248 4556
Fragments arranged by size:
4556 763 248 18
NcoI C'CATG_G
Cuts at: 642 1590 642
Size: 948 4637
NgoMIV G'CCGG_C
Cuts at: 1002 3764 1002
Size: 2762 2823
NspI r_CATG'y
Cuts at: 495 1050 2638 3138 4045 495
Size: 555 1588 500 907 2035
Fragments arranged by size:
2035 1588 907 555 500
PasI CC'CwG_GG
Cuts at: 138 138
Size: 5585
PciI A'CATG_T
Cuts at: 4041 4041
Size: 5585
PflMI CCAn_nnn'nTGG
Cuts at: 465 1473 2021 465
Size: 1008 548 4029
Fragments arranged by size:
4029 1008 548
PfoI T'CCnGG_A
Cuts at: 412 797 412
Size: 385 5200
PmeI GTTT'AAAC
Cuts at: 2584 2584
Size: 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4724 4756 4724
Size: 32 5553
PpuMI rG'GwC_Cy
Cuts at: 351 855 1602 2079 351
Size: 504 747 477 3857
Fragments arranged by size:
3857 747 504 477
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1075 1107 1075
Size: 32 5553
PstI C_TGCA'G
Cuts at: 2324 2366 2632 3317 2324
Size: 42 266 685 4592
Fragments arranged by size:
4592 685 266 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5585
PvuII CAG'CTG
Cuts at: 983 1574 2334 3010 3370 983
Size: 591 760 676 360 3198
Fragments arranged by size:
3198 760 676 591 360
RsrII CG'GwC_CG
Cuts at: 3780 3780
Size: 5585
SacI G_AGCT'C
Cuts at: 1103 1646 2599 1103
Size: 543 953 4089
Fragments arranged by size:
4089 953 543
SalI G'TCGA_C
Cuts at: 2622 2622
Size: 5585
SanDI GG'GwC_CC
Cuts at: 351 855 351
Size: 504 5081
SapI GCTCTTCn'nnn_
Cuts at: 1651 3608 3818 1651
Size: 1957 210 3418
Fragments arranged by size:
3418 1957 210
SbfI CC_TGCA'GG
Cuts at: 2632 2632
Size: 5585
ScaI AGT'ACT
Cuts at: 343 5102 343
Size: 4759 826
SfcI C'TryA_G
Cuts at: 33 2320 2362 2628 3313 4306 4497 33
Size: 2287 42 266 685 993 191 1121
Fragments arranged by size:
2287 1121 993 685 266 191 42
SfoI GGC'GCC
Cuts at: 2236 2254 3017 3265 2236
Size: 18 763 248 4556
Fragments arranged by size:
4556 763 248 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
SmaI CCC'GGG
Cuts at: 2607 2607
Size: 5585
SmlI C'TyrA_G
Cuts at: 2740 3655 4147 4409 2740
Size: 915 492 262 3916
Fragments arranged by size:
3916 915 492 262
SnaBI TAC'GTA
Cuts at: 2577 2577
Size: 5585
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5585
SphI G_CATG'C
Cuts at: 2638 3138 2638
Size: 500 5085
StuI AGG'CCT
Cuts at: 1253 1253
Size: 5585
StyI C'CwwG_G
Cuts at: 642 1242 1590 2719 3073 642
Size: 600 348 1129 354 3154
Fragments arranged by size:
3154 1129 600 354 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1621 3168 3301 3760 3969 1461
Size: 160 1547 133 459 209 3077
Fragments arranged by size:
3077 1547 459 209 160 133
TatI w'GTAC_w
Cuts at: 341 1010 1394 2213 2536 5100 341
Size: 669 384 819 323 2564 826
Fragments arranged by size:
2564 826 819 669 384 323
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1947 1979 2454 2486 2542 2574 5102 5134 1947
Size: 32 475 32 56 32 2528 32 2398
Fragments arranged by size:
2528 2398 475 56 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 918 1033 3382 918
Size: 115 2349 3121
Fragments arranged by size:
3121 2349 115
XbaI T'CTAG_A
Cuts at: 2616 2616
Size: 5585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 321 3080 321
Size: 2759 2826
XmaI C'CCGG_G
Cuts at: 2605 2605
Size: 5585
XmnI GAAnn'nnTTC
Cuts at: 1904 1904
Size: 5585
ZraI GAC'GTC
Cuts at: 1167 1674 1167
Size: 507 5078
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BssHII ClaI DraIII EcoNI FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SrfI
SspI SwaI XhoI