Restriction map for
pF1KSDA1263
(Circular) (Six-base) MAPSORT of: pF1KSDA1263.seq Check: 2676 from: 1 to: 5675
pF1KSDA1263 5675 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1090 1090
Size: 5675
AccI GT'mk_AC
Cuts at: 2713 2713
Size: 5675
Acc65I G'GTAC_C
Cuts at: 1807 2691 1807
Size: 884 4791
AclI AA'CG_TT
Cuts at: 5515 5515
Size: 5675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2642 3500 3932 4679 4947 2390
Size: 252 858 432 747 268 3118
Fragments arranged by size:
3118 858 747 432 268 252
AflIII A'CryG_T
Cuts at: 1478 3027 4131 5108 1478
Size: 1549 1104 977 2045
Fragments arranged by size:
2045 1549 1104 977
AgeI A'CCGG_T
Cuts at: 4063 4063
Size: 5675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 488
Size: 5675
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1598 1630 1598
Size: 32 5643
AlwNI CAG_nnn'CTG
Cuts at: 904 1747 2980 4547 904
Size: 843 1233 1567 2032
Fragments arranged by size:
2032 1567 1233 843
ApaLI G'TGCA_C
Cuts at: 463 4445 463
Size: 3982 1693
ApoI r'AATT_y
Cuts at: 439 832 1534 2679 5028 5284 439
Size: 393 702 1145 2349 256 830
Fragments arranged by size:
2349 1145 830 702 393 256
AseI AT'TA_AT
Cuts at: 20 713 2249 20
Size: 693 1536 3446
Fragments arranged by size:
3446 1536 693
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
AvaI C'yCGr_G
Cuts at: 2695 2695
Size: 5675
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1344 1377 1344
Size: 33 5642
BamHI G'GATC_C
Cuts at: 2700 2700
Size: 5675
BanI G'GyrC_C
Cuts at: 1205 1807 2691 3105 3353 3388 1205
Size: 602 884 414 248 35 3492
Fragments arranged by size:
3492 884 602 414 248 35
BanII G_rGCy'C
Cuts at: 2435 2689 3719 2435
Size: 254 1030 4391
Fragments arranged by size:
4391 1030 254
BbeI G_GCGC'C
Cuts at: 3109 3357 3109
Size: 248 5427
BbsI GAAGACnn'nnnn_
Cuts at: 348 521 1013 1397 1421 1825 348
Size: 173 492 384 24 404 4198
Fragments arranged by size:
4198 492 404 384 173 24
BbvCI CC'TCA_GC
Cuts at: 1232 1601 1232
Size: 369 5306
BciVI GTATCCnnnnn_n'
Cuts at: 1169 3573 4334 5675 1169
Size: 2404 761 1341 1169
Fragments arranged by size:
2404 1341 1169 761
BclI T'GATC_A
Cuts at: 1782 3195 1782
Size: 1413 4262
BglII A'GATC_T
Cuts at: 3190 5227 3190
Size: 2037 3638
BlpI GC'TnA_GC
Cuts at: 1798 2787 1798
Size: 989 4686
Bme1580I G_kGCm'C
Cuts at: 467 2209 3300 3393 4449 467
Size: 1742 1091 93 1056 1693
Fragments arranged by size:
1742 1693 1091 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 2887 2968 3303 5447 1747
Size: 1140 81 335 2144 1975
Fragments arranged by size:
2144 1975 1140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4042 4042
Size: 5675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1127 1367 2207 193
Size: 934 240 840 3661
Fragments arranged by size:
3661 934 840 240
Bpu10I CC'TnA_GC
Cuts at: 1232 1601 1232
Size: 369 5306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 2464 2851 3730 4222 4520 2452
Size: 12 387 879 492 298 3607
Fragments arranged by size:
3607 879 492 387 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 945 5379 945
Size: 4434 1241
BsaAI yAC'GTr
Cuts at: 2667 3028 2667
Size: 361 5314
BsaBI GATnn'nnATC
Cuts at: 6 3189 5226 6
Size: 3183 2037 455
Fragments arranged by size:
3183 2037 455
BsaHI Gr'CG_yC
Cuts at: 3106 3354 4056 5552 3106
Size: 248 702 1496 3229
Fragments arranged by size:
3229 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2131 2504 2864 3385 4063 4337 4484 4895 2131
Size: 373 360 521 678 274 147 411 2911
Fragments arranged by size:
2911 678 521 411 373 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1598 1628 2193 2223 1598
Size: 30 565 30 5050
Fragments arranged by size:
5050 565 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2309 2309
Size: 5675
BseYI C'CCAG_C
Cuts at: 1802 3100 3858 4435 4931 1802
Size: 1298 758 577 496 2546
Fragments arranged by size:
2546 1298 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 1979 2317 1729
Size: 250 338 5087
Fragments arranged by size:
5087 338 250
BsiEI CG_ry'CG
Cuts at: 104 3217 3263 4471 104
Size: 3113 46 1208 1308
Fragments arranged by size:
3113 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 1741 2423 2435 2689 3467 3657 4449 467
Size: 1274 682 12 254 778 190 792 1693
Fragments arranged by size:
1693 1274 792 778 682 254 190 12
BsmI GAATG_Cn'
Cuts at: 844 844
Size: 5675
Bsp1286I G_dGCh'C
Cuts at: 467 1741 2209 2423 2435 2689 3300 3393 3467
Size: 1274 468 214 12 254 611 93 74
Cuts at: 3467 3657 3719 4449 467
Size: 190 62 730 1693
Fragments arranged by size:
1693 1274 730 611 468 254 214 190
93 74 62 12
BspEI T'CCGG_A
Cuts at: 4895 4895
Size: 5675
BspHI T'CATG_A
Cuts at: 4851 4851
Size: 5675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 2725 2939 3241 3622 1090
Size: 1635 214 302 381 3143
Fragments arranged by size:
3143 1635 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3967 4021 5505 5670 50
Size: 3917 54 1484 165 55
Fragments arranged by size:
3917 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1332 3587 1332
Size: 2255 3420
BsrFI r'CCGG_y
Cuts at: 3673 3854 4063 3673
Size: 181 209 5285
Fragments arranged by size:
5285 209 181
BssSI C'ACGA_G
Cuts at: 3946 4304 3946
Size: 358 5317
BstAPI GCAn_nnn'nTGC
Cuts at: 382 3052 382
Size: 2670 3005
BstBI TT'CG_AA
Cuts at: 110 4036 110
Size: 3926 1749
BstEII G'GTnAC_C
Cuts at: 1053 1053
Size: 5675
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2530
Size: 5675
BstYI r'GATC_y
Cuts at: 1 310 427 756 2700 3167 3190 3525 3771
Size: 309 117 329 1944 467 23 335 246
Cuts at: 3771 4783 4869 4881 5227 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1944 1012 467 449 346 335 329 309
246 117 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3625 3806 3969 3625
Size: 181 163 5331
Fragments arranged by size:
5331 181 163
BtsI GCAGTG_nn'
Cuts at: 893 1314 1739 2918 893
Size: 421 425 1179 3650
Fragments arranged by size:
3650 1179 425 421
DraI TTT'AAA
Cuts at: 528 675 2674 528
Size: 147 1999 3529
Fragments arranged by size:
3529 1999 147
DrdI GACnn_nn'nnGTC
Cuts at: 3381 4239 3381
Size: 858 4817
EaeI y'GGCC_r
Cuts at: 1850 2229 3260 3434 3825 3852 5197 1850
Size: 379 1031 174 391 27 1345 2328
Fragments arranged by size:
2328 1345 1031 391 379 174 27
EagI C'GGCC_G
Cuts at: 3260 3260
Size: 5675
EarI CTCTTCn'nnn_
Cuts at: 1404 2442 2581 3698 3908 1404
Size: 1038 139 1117 210 3171
Fragments arranged by size:
3171 1117 1038 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3883 4193 4339 5485 3883
Size: 310 146 1146 4073
Fragments arranged by size:
4073 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2433 2687 2433
Size: 254 5421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1127 1367 2207 2390 2642 3500 3932 4679
Size: 934 240 840 183 252 858 432 747
Cuts at: 4679 4947 193
Size: 268 921
Fragments arranged by size:
934 921 858 840 747 432 268 252
240 183
EcoO109I rG'GnC_Cy
Cuts at: 1034 2814 1034
Size: 1780 3895
EcoRI G'AATT_C
Cuts at: 439 2679 439
Size: 2240 3435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 777 3694 777
Size: 2917 2758
FspI TGC'GCA
Cuts at: 3456 3456
Size: 5675
HaeII r_GCGC'y
Cuts at: 3109 3357 4379 5358 3109
Size: 248 1022 979 3426
Fragments arranged by size:
3426 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 1018 1050 1696 1728 1807 1839 2193
Size: 32 361 32 646 32 79 32 354
Cuts at: 2193 2225 2448 2480 3517 3547 3549 3579 625
Size: 32 223 32 1037 30 2 30 2721
Fragments arranged by size:
2721 1037 646 361 354 223 79 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2714 2714
Size: 5675
HindIII A'AGCT_T
Cuts at: 1273 1273
Size: 5675
KasI G'GCGC_C
Cuts at: 3105 3353 3105
Size: 248 5427
KpnI G_GTAC'C
Cuts at: 1811 2695 1811
Size: 884 4791
MluI A'CGCG_T
Cuts at: 5108 5108
Size: 5675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 158 2634 4346 4530 158
Size: 2476 1712 184 1303
Fragments arranged by size:
2476 1712 1303 184
MscI TGG'CCA
Cuts at: 1852 2231 3436 1852
Size: 379 1205 4091
Fragments arranged by size:
4091 1205 379
MslI CAynn'nnrTG
Cuts at: 1153 1957 1985 3664 3791 1153
Size: 804 28 1679 127 3037
Fragments arranged by size:
3037 1679 804 127 28
MspA1I CmG'CkG
Cuts at: 1639 2786 3100 3460 4473 4718 1639
Size: 1147 314 360 1013 245 2596
Fragments arranged by size:
2596 1147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3856 3856
Size: 5675
NarI GG'CG_CC
Cuts at: 3106 3354 3106
Size: 248 5427
NdeI CA'TA_TG
Cuts at: 1958 2214 1958
Size: 256 5419
NgoMIV G'CCGG_C
Cuts at: 3854 3854
Size: 5675
NspI r_CATG'y
Cuts at: 1482 2728 3228 4135 1482
Size: 1246 500 907 3022
Fragments arranged by size:
3022 1246 907 500
PciI A'CATG_T
Cuts at: 1478 4131 1478
Size: 2653 3022
PflMI CCAn_nnn'nTGG
Cuts at: 965 1893 965
Size: 928 4747
PfoI T'CCnGG_A
Cuts at: 2522 2522
Size: 5675
PmeI GTTT'AAAC
Cuts at: 2674 2674
Size: 5675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1598 1630 4814 4846 1598
Size: 32 3184 32 2427
Fragments arranged by size:
3184 2427 32 32
PpuMI rG'GwC_Cy
Cuts at: 1034 1034
Size: 5675
PstI C_TGCA'G
Cuts at: 900 2722 3407 900
Size: 1822 685 3168
Fragments arranged by size:
3168 1822 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5675
PvuII CAG'CTG
Cuts at: 1639 3100 3460 1639
Size: 1461 360 3854
Fragments arranged by size:
3854 1461 360
RsrII CG'GwC_CG
Cuts at: 3870 3870
Size: 5675
SacI G_AGCT'C
Cuts at: 2435 2689 2435
Size: 254 5421
SalI G'TCGA_C
Cuts at: 2712 2712
Size: 5675
SapI GCTCTTCn'nnn_
Cuts at: 3698 3908 3698
Size: 210 5465
SbfI CC_TGCA'GG
Cuts at: 2722 2722
Size: 5675
ScaI AGT'ACT
Cuts at: 328 5192 328
Size: 4864 811
SfcI C'TryA_G
Cuts at: 33 896 1834 2718 3403 4396 4587 33
Size: 863 938 884 685 993 191 1121
Fragments arranged by size:
1121 993 938 884 863 685 191
SfoI GGC'GCC
Cuts at: 3107 3355 3107
Size: 248 5427
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
SmaI CCC'GGG
Cuts at: 2697 2697
Size: 5675
SmlI C'TyrA_G
Cuts at: 2467 2479 2830 3745 4237 4499 2467
Size: 12 351 915 492 262 3643
Fragments arranged by size:
3643 915 492 351 262 12
SnaBI TAC'GTA
Cuts at: 2667 2667
Size: 5675
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5675
SphI G_CATG'C
Cuts at: 2728 3228 2728
Size: 500 5175
SspI AAT'ATT
Cuts at: 679 981 679
Size: 302 5373
StyI C'CwwG_G
Cuts at: 1569 1811 2497 2564 2809 3163 1569
Size: 242 686 67 245 354 4081
Fragments arranged by size:
4081 686 354 245 242 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1782 1900 1969 3258 3391 3850 4059 1782
Size: 118 69 1289 133 459 209 3398
Fragments arranged by size:
3398 1289 459 209 133 118 69
TatI w'GTAC_w
Cuts at: 326 769 2279 5190 326
Size: 443 1510 2911 811
Fragments arranged by size:
2911 1510 811 443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1496 1528 2044 2076 5192 5224 1496
Size: 32 516 32 3116 32 1947
Fragments arranged by size:
3116 1947 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 1747 2176 3472 1690
Size: 57 429 1296 3893
Fragments arranged by size:
3893 1296 429 57
XbaI T'CTAG_A
Cuts at: 2706 2706
Size: 5675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1963 3170 1963
Size: 1207 4468
XmaI C'CCGG_G
Cuts at: 2695 2695
Size: 5675
XmnI GAAnn'nnTTC
Cuts at: 1480 1480
Size: 5675
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI